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Database: PDB
Entry: 1WWX
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Original site: 1WWX 
HEADER    DNA BINDING PROTEIN                     18-JAN-05   1WWX              
TITLE     SOLUTION STRUCTURE OF THE ETS-DOMAIN OF THE ETS DOMAIN                
TITLE    2 TRANSCRIPTION FACTOR                                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: E74-LIKE FACTOR 5 ESE-2B;                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: ETS DOMAIN;                                                
COMPND   5 SYNONYM: ETS DOMAIN TRANSCRIPTION FACTOR;                            
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ELF5;                                                          
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040614-12;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    DNA BINDING, TRANSCRIPTIONAL ACTIVATION AND REPRESSION, E74-          
KEYWDS   2 LIKE FACTOR 5, STRUCTURAL GENOMICS, RIKEN STRUCTURAL                 
KEYWDS   3 GENOMICS/PROTEOMICS INITIATIVE, RSGI, DNA BINDING PROTEIN            
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    A.GORONCY,T.KIGAWA,S.KOSHIBA,N.KOBAYASHI,N.TOCHIO,M.INOUE,            
AUTHOR   2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE           
AUTHOR   3 (RSGI)                                                               
REVDAT   2   24-FEB-09 1WWX    1       VERSN                                    
REVDAT   1   18-JUL-05 1WWX    0                                                
JRNL        AUTH   A.GORONCY,T.KIGAWA,S.KOSHIBA,N.KOBAYASHI,N.TOCHIO,           
JRNL        AUTH 2 M.INOUE,S.YOKOYAMA                                           
JRNL        TITL   SOLUTION STRUCTURE OF THE ETS-DOMAIN OF THE ETS              
JRNL        TITL 2 DOMAIN TRANSCRIPTION FACTOR                                  
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.26                                         
REMARK   3   AUTHORS     : PETER GUENTERT                                       
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1WWX COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 20-JAN-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB024097.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 220MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.75MM ETS DOMAIN, 20MM TRIS-      
REMARK 210                                   HCL, 200MM NACL, 1MM D-DTT,        
REMARK 210                                   0.02% NAN3                         
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 2.6, NMRPIPE               
REMARK 210                                   20020425, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.896                       
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION                    
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   3      -54.63   -142.44                                   
REMARK 500  1 VAL A  41       79.55   -101.44                                   
REMARK 500  1 ASP A  82       33.88    -96.31                                   
REMARK 500  1 ASP A  99      170.26     53.72                                   
REMARK 500  2 SER A   6       92.77     53.11                                   
REMARK 500  2 SER A   9       93.69     48.92                                   
REMARK 500  2 ASP A  33       96.29   -167.97                                   
REMARK 500  2 ASP A  82       41.42    -96.34                                   
REMARK 500  2 ASP A  99      126.15   -177.70                                   
REMARK 500  2 SER A 105      119.89    -34.95                                   
REMARK 500  3 SER A   3      -52.84   -176.48                                   
REMARK 500  3 SER A   6      -54.03   -161.66                                   
REMARK 500  3 ASP A  33      110.79   -160.91                                   
REMARK 500  3 ASP A  58      -22.42     83.46                                   
REMARK 500  3 ASP A  82       37.80    -99.54                                   
REMARK 500  3 GLN A  97      163.85    -47.84                                   
REMARK 500  3 GLU A  98      137.48    -35.50                                   
REMARK 500  3 LEU A 101      115.69   -164.71                                   
REMARK 500  3 SER A 106      -46.47   -171.06                                   
REMARK 500  4 SER A   2      110.53   -170.58                                   
REMARK 500  4 SER A   3      173.76    -57.55                                   
REMARK 500  4 SER A   6       43.86   -168.70                                   
REMARK 500  4 SER A   9       85.40   -163.80                                   
REMARK 500  4 ASP A  33       96.64   -162.27                                   
REMARK 500  4 ASP A  82       34.24    -92.72                                   
REMARK 500  4 LEU A  85       27.63     48.70                                   
REMARK 500  4 PRO A 104     -178.08    -69.73                                   
REMARK 500  4 SER A 105      -56.69   -178.47                                   
REMARK 500  5 SER A   2      143.09   -173.61                                   
REMARK 500  5 ASP A  33      105.06   -169.71                                   
REMARK 500  5 ASP A  58      -34.06     83.59                                   
REMARK 500  5 SER A 102       80.42     51.44                                   
REMARK 500  5 SER A 105      -50.95   -144.93                                   
REMARK 500  6 SER A   6      -49.98   -166.47                                   
REMARK 500  6 SER A   9       35.19   -154.88                                   
REMARK 500  6 ASP A  82       43.32    -86.18                                   
REMARK 500  6 GLU A  98       86.75   -163.99                                   
REMARK 500  6 ASP A  99      166.67     69.27                                   
REMARK 500  6 LYS A 100       72.63     39.23                                   
REMARK 500  6 SER A 105     -173.52     58.46                                   
REMARK 500  7 SER A   5       86.94     35.40                                   
REMARK 500  7 ASP A  33      105.91   -172.72                                   
REMARK 500  7 LYS A  56       29.48     43.69                                   
REMARK 500  7 ASP A  58      -33.42     83.73                                   
REMARK 500  7 SER A 106      105.20   -169.74                                   
REMARK 500  8 SER A   5      124.30   -170.42                                   
REMARK 500  8 SER A   6       98.53   -161.22                                   
REMARK 500  8 SER A   8       85.45     58.80                                   
REMARK 500  8 SER A   9      -48.05   -172.99                                   
REMARK 500  8 ASP A  33      105.53   -162.40                                   
REMARK 500  8 ARG A  59       40.04   -105.11                                   
REMARK 500  8 ASP A  99      133.34     62.64                                   
REMARK 500  9 SER A   2      102.95    -54.33                                   
REMARK 500  9 SER A   3      104.84   -176.47                                   
REMARK 500  9 HIS A  10      131.93     65.34                                   
REMARK 500  9 ASP A  33      104.86   -166.31                                   
REMARK 500  9 SER A 105      -59.05   -133.74                                   
REMARK 500 10 SER A   6      -43.83   -171.99                                   
REMARK 500 10 SER A   8       97.89     47.60                                   
REMARK 500 10 HIS A  10      123.04     62.70                                   
REMARK 500 10 ASP A  33      102.50   -167.84                                   
REMARK 500 10 ASP A  58      -33.06     83.71                                   
REMARK 500 10 ASP A  82       44.25    -94.49                                   
REMARK 500 10 ASP A  99      161.34     56.62                                   
REMARK 500 10 LEU A 101      173.42    178.72                                   
REMARK 500 10 SER A 102       90.49   -161.35                                   
REMARK 500 11 SER A   2      142.13   -173.52                                   
REMARK 500 11 SER A   3      156.02     69.15                                   
REMARK 500 11 SER A   5      110.41   -167.39                                   
REMARK 500 11 SER A   9      -58.54     78.65                                   
REMARK 500 11 ASP A  33      106.28   -166.25                                   
REMARK 500 11 ASP A  99      137.46     61.21                                   
REMARK 500 11 SER A 106       92.92     45.48                                   
REMARK 500 12 SER A   5       41.42     71.97                                   
REMARK 500 12 SER A   6      -42.40    179.85                                   
REMARK 500 12 ASP A  33       90.69   -166.82                                   
REMARK 500 12 LYS A  56       33.29     36.44                                   
REMARK 500 12 LEU A  85       30.46     37.02                                   
REMARK 500 12 SER A 102      -59.65   -170.13                                   
REMARK 500 13 ASP A  33      104.22   -162.06                                   
REMARK 500 13 TYR A  62      -35.88    -38.86                                   
REMARK 500 13 GLN A  97      157.79    -46.81                                   
REMARK 500 13 LYS A 100      172.02    -50.79                                   
REMARK 500 13 SER A 102      151.72     58.89                                   
REMARK 500 14 SER A   2       42.50   -143.63                                   
REMARK 500 14 ASP A  33      105.47   -161.87                                   
REMARK 500 14 ASN A  57      -88.63    -58.67                                   
REMARK 500 14 ASP A  58      -55.92   -179.44                                   
REMARK 500 14 ARG A  59       37.77   -149.61                                   
REMARK 500 14 MET A  60      140.49    -34.51                                   
REMARK 500 14 TYR A  62      -32.37    -37.40                                   
REMARK 500 14 ASP A  82       32.28    -94.31                                   
REMARK 500 14 ASP A  99      178.63     58.35                                   
REMARK 500 14 LEU A 101      126.08    177.93                                   
REMARK 500 14 SER A 102       95.06     62.95                                   
REMARK 500 14 SER A 105      154.27     62.95                                   
REMARK 500 14 SER A 106      101.10   -162.56                                   
REMARK 500 15 SER A   2      113.03   -168.47                                   
REMARK 500 15 SER A   3       92.65    -58.34                                   
REMARK 500 15 SER A   6       88.34   -156.75                                   
REMARK 500 15 SER A   9      -53.68    178.62                                   
REMARK 500 15 HIS A  10      124.51     60.76                                   
REMARK 500 15 GLU A  98      100.15    -48.59                                   
REMARK 500 15 PRO A 104       91.76    -69.77                                   
REMARK 500 15 SER A 105      -42.82    172.92                                   
REMARK 500 15 SER A 106      -57.89   -168.68                                   
REMARK 500 16 SER A   2       97.57   -162.66                                   
REMARK 500 16 HIS A  10      131.36     72.13                                   
REMARK 500 16 ASP A  33       83.19   -165.68                                   
REMARK 500 16 ASP A  82       44.75    -93.99                                   
REMARK 500 16 GLN A  97      165.75    -45.82                                   
REMARK 500 16 GLU A  98      178.98     53.93                                   
REMARK 500 16 ASP A  99      149.27     62.68                                   
REMARK 500 16 LYS A 100       67.76     38.21                                   
REMARK 500 16 SER A 102      101.84   -179.52                                   
REMARK 500 17 SER A   2      -46.41   -146.58                                   
REMARK 500 17 SER A   9     -167.20   -161.94                                   
REMARK 500 17 HIS A  10      157.91     59.06                                   
REMARK 500 17 ASP A  58      -37.04     82.93                                   
REMARK 500 17 LEU A  85       43.00     37.92                                   
REMARK 500 17 GLN A  97      170.58    -45.69                                   
REMARK 500 17 LYS A 100      148.75    -34.38                                   
REMARK 500 17 SER A 105       76.87     49.12                                   
REMARK 500 18 SER A   5      108.20   -172.57                                   
REMARK 500 18 SER A   6      169.08    178.58                                   
REMARK 500 18 ARG A  59       40.55   -104.72                                   
REMARK 500 18 ASP A  82       39.78    -83.62                                   
REMARK 500 18 LEU A 101      122.39     62.99                                   
REMARK 500 18 SER A 102      -59.74   -172.38                                   
REMARK 500 18 PRO A 104     -179.17    -69.77                                   
REMARK 500 19 SER A   3      -57.18   -166.68                                   
REMARK 500 19 SER A   6      132.13   -178.15                                   
REMARK 500 19 SER A   9      -40.85   -148.76                                   
REMARK 500 19 ASP A  58      -34.13     83.64                                   
REMARK 500 19 ASP A  82       32.13    -95.92                                   
REMARK 500 19 ASP A  99      154.38     58.31                                   
REMARK 500 19 SER A 105      -46.62   -169.36                                   
REMARK 500 19 SER A 106      176.17    172.71                                   
REMARK 500 20 SER A   9       45.73   -159.65                                   
REMARK 500 20 ASP A  33      102.90   -163.42                                   
REMARK 500 20 ASP A  58      -32.92     83.72                                   
REMARK 500 20 ASP A  82       45.50    -98.33                                   
REMARK 500 20 GLN A  97      169.21    -48.42                                   
REMARK 500 20 LYS A 100      152.21     61.31                                   
REMARK 500 20 SER A 102      157.91     61.83                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSS001002867.1   RELATED DB: TARGETDB                    
DBREF  1WWX A    8   101  GB     4557551  NP_001413      162    255             
SEQADV 1WWX GLY A    1  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A    2  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A    3  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX GLY A    4  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A    5  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A    6  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX GLY A    7  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A  102  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX GLY A  103  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX PRO A  104  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A  105  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX SER A  106  GB   4557551             CLONING ARTIFACT               
SEQADV 1WWX GLY A  107  GB   4557551             CLONING ARTIFACT               
SEQRES   1 A  107  GLY SER SER GLY SER SER GLY SER SER HIS LEU TRP GLU          
SEQRES   2 A  107  PHE VAL ARG ASP LEU LEU LEU SER PRO GLU GLU ASN CYS          
SEQRES   3 A  107  GLY ILE LEU GLU TRP GLU ASP ARG GLU GLN GLY ILE PHE          
SEQRES   4 A  107  ARG VAL VAL LYS SER GLU ALA LEU ALA LYS MET TRP GLY          
SEQRES   5 A  107  GLN ARG LYS LYS ASN ASP ARG MET THR TYR GLU LYS LEU          
SEQRES   6 A  107  SER ARG ALA LEU ARG TYR TYR TYR LYS THR GLY ILE LEU          
SEQRES   7 A  107  GLU ARG VAL ASP ARG ARG LEU VAL TYR LYS PHE GLY LYS          
SEQRES   8 A  107  ASN ALA HIS GLY TRP GLN GLU ASP LYS LEU SER GLY PRO          
SEQRES   9 A  107  SER SER GLY                                                  
HELIX    1   1 HIS A   10  SER A   21  1                                  12    
HELIX    2   2 LYS A   43  LYS A   55  1                                  13    
HELIX    3   3 THR A   61  GLY A   76  1                                  16    
SHEET    1   A 4 GLU A  30  ASP A  33  0                                        
SHEET    2   A 4 ILE A  38  ARG A  40 -1  O  ILE A  38   N  ASP A  33           
SHEET    3   A 4 VAL A  86  PHE A  89 -1  O  TYR A  87   N  PHE A  39           
SHEET    4   A 4 LEU A  78  GLU A  79 -1  N  GLU A  79   O  LYS A  88           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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