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Database: PDB
Entry: 1X1F
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HEADER    SIGNALING PROTEIN                       04-APR-05   1X1F              
TITLE     SOLUTION STRUCTURE OF THE PH DOMAIN OF HUMAN DOCKING                  
TITLE    2 PROTEIN BRDG1                                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SIGNAL-TRANSDUCING ADAPTOR PROTEIN 1;                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: PH DOMAIN;                                                 
COMPND   5 SYNONYM: STAP-1, STEM CELL ADAPTOR PROTEIN 1, BCR                    
COMPND   6 DOWNSTREAM SIGNALING PROTEIN 1, DOCKING PROTEIN BRDG1;               
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: BRDG1;                                                         
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040301-57;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    DOCKING PROTEIN BRDG1, PH DOMAIN, STRUCTURAL GENOMICS,                
KEYWDS   2 NPPSFA, NATIONAL PROJECT ON PROTEIN STRUCTURAL AND                   
KEYWDS   3 FUNCTIONAL ANALYSES, RIKEN STRUCTURAL GENOMICS/PROTEOMICS            
KEYWDS   4 INITIATIVE, RSGI, SIGNALING PROTEIN                                  
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    H.LI,T.TOMIZAWA,S.KOSHIBA,M.INOUE,T.KIGAWA,S.YOKOYAMA,RIKEN           
AUTHOR   2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)                     
REVDAT   2   24-FEB-09 1X1F    1       VERSN                                    
REVDAT   1   04-OCT-05 1X1F    0                                                
JRNL        AUTH   H.LI,T.TOMIZAWA,S.KOSHIBA,M.INOUE,T.KIGAWA,                  
JRNL        AUTH 2 S.YOKOYAMA                                                   
JRNL        TITL   SOLUTION STRUCTURE OF THE PH DOMAIN OF HUMAN                 
JRNL        TITL 2 DOCKING PROTEIN BRDG1                                        
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 1.0.7                                          
REMARK   3   AUTHORS     : GUENTERT, P.                                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1X1F COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 06-APR-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB024257.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 296                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.00MM PH DOMAIN U-13C, 15N;       
REMARK 210                                   20MM D-TRIS-HCL(PH7.0); 100MM      
REMARK 210                                   NACL; 1MM D-DTT; 0.02% NAN3;       
REMARK 210                                   90% H2O, 10% D2O                   
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 2.6, NMRPIPE               
REMARK 210                                   20031121, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.913, CYANA 1.0.7          
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION,STRUCTURES         
REMARK 210                                   WITH THE LEAST RESTRAINT           
REMARK 210                                   VIOLATIONS                         
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 THR A  14      -69.22   -122.43                                   
REMARK 500  1 ALA A  15      -60.85   -146.63                                   
REMARK 500  1 PHE A  23      108.25    -55.85                                   
REMARK 500  1 SER A  29      112.37    -39.58                                   
REMARK 500  1 ARG A  32      -46.18     84.74                                   
REMARK 500  1 TYR A  34      130.10    -36.32                                   
REMARK 500  1 THR A  44       19.16   -146.82                                   
REMARK 500  1 LEU A  61     -161.91   -120.54                                   
REMARK 500  1 CYS A  68     -179.81    167.94                                   
REMARK 500  1 SER A  74       88.21     49.10                                   
REMARK 500  1 GLU A  76      -59.34   -169.74                                   
REMARK 500  1 THR A  95     -161.61   -122.41                                   
REMARK 500  1 THR A 112      -48.84   -143.47                                   
REMARK 500  1 SER A 115     -156.64   -141.90                                   
REMARK 500  1 ASN A 119       57.23   -109.85                                   
REMARK 500  1 LEU A 122     -176.99    -56.19                                   
REMARK 500  1 ILE A 142      -79.62    -43.27                                   
REMARK 500  1 SER A 144      -53.57   -124.53                                   
REMARK 500  1 SER A 148      163.44     64.43                                   
REMARK 500  2 SER A   2      169.17    179.77                                   
REMARK 500  2 SER A   5       89.75     61.64                                   
REMARK 500  2 SER A   6      111.17   -170.99                                   
REMARK 500  2 GLN A   8       76.20     46.74                                   
REMARK 500  2 LEU A  11      105.77     64.81                                   
REMARK 500  2 PHE A  20      144.94   -176.79                                   
REMARK 500  2 PHE A  23       99.60    -44.11                                   
REMARK 500  2 ARG A  28      -80.89    -40.98                                   
REMARK 500  2 SER A  29      -55.74   -161.28                                   
REMARK 500  2 GLU A  33      148.25   -178.13                                   
REMARK 500  2 TYR A  34      123.94    -37.49                                   
REMARK 500  2 TRP A  38       87.89    -65.81                                   
REMARK 500  2 THR A  44       20.21   -150.16                                   
REMARK 500  2 CYS A  68     -179.32    164.44                                   
REMARK 500  2 SER A  74      -59.16    179.93                                   
REMARK 500  2 GLU A  76      173.25    -50.12                                   
REMARK 500  2 ASN A  78      -49.54    178.20                                   
REMARK 500  2 THR A 112      -45.18   -147.92                                   
REMARK 500  2 GLN A 118       30.78    -90.85                                   
REMARK 500  2 LEU A 122     -179.34    -50.95                                   
REMARK 500  2 ILE A 142      -78.97    -43.27                                   
REMARK 500  3 SER A   2       97.05   -175.30                                   
REMARK 500  3 SER A   6      -57.84   -121.98                                   
REMARK 500  3 ILE A  13      142.41     65.21                                   
REMARK 500  3 PHE A  20      144.08   -176.09                                   
REMARK 500  3 PHE A  23       98.91    -37.53                                   
REMARK 500  3 SER A  29       90.90    -65.41                                   
REMARK 500  3 CYS A  68     -178.65   -179.11                                   
REMARK 500  3 SER A  74      -61.21   -140.70                                   
REMARK 500  3 THR A  75      121.57     62.90                                   
REMARK 500  3 GLU A  76      -58.47     72.44                                   
REMARK 500  3 THR A  95     -159.23   -119.24                                   
REMARK 500  3 GLU A  96      -67.31    -96.33                                   
REMARK 500  3 VAL A 111       30.45    -91.59                                   
REMARK 500  3 THR A 112      -45.39   -149.26                                   
REMARK 500  3 LEU A 122     -178.44    -55.36                                   
REMARK 500  3 LEU A 134      -70.85    -55.44                                   
REMARK 500  3 ILE A 142      -80.30    -44.42                                   
REMARK 500  3 SER A 144      -60.93     69.86                                   
REMARK 500  3 SER A 147      171.16     55.68                                   
REMARK 500  3 SER A 148      137.87    176.00                                   
REMARK 500  4 SER A   3      102.61   -178.69                                   
REMARK 500  4 LEU A  11     -179.84    -68.65                                   
REMARK 500  4 TYR A  19      -52.09   -122.63                                   
REMARK 500  4 PHE A  23      100.89    -38.93                                   
REMARK 500  4 ARG A  28     -169.97    -54.06                                   
REMARK 500  4 GLU A  33      165.46    175.23                                   
REMARK 500  4 TRP A  38       90.21    -67.58                                   
REMARK 500  4 THR A  44       45.32   -148.50                                   
REMARK 500  4 ASP A  51      -70.31   -167.54                                   
REMARK 500  4 LYS A  52      -61.02   -172.20                                   
REMARK 500  4 TYR A  57      136.94    -37.39                                   
REMARK 500  4 CYS A  68     -179.35    167.30                                   
REMARK 500  4 LYS A  77     -152.63     39.63                                   
REMARK 500  4 GLU A  96      -53.91     77.85                                   
REMARK 500  4 ARG A 105      -36.48    -39.34                                   
REMARK 500  4 THR A 112      -44.57   -146.51                                   
REMARK 500  4 LEU A 122     -179.38    -51.86                                   
REMARK 500  4 ILE A 142      -79.88    -43.76                                   
REMARK 500  5 SER A   2      148.52   -171.04                                   
REMARK 500  5 SER A   3       93.01   -176.81                                   
REMARK 500  5 ALA A  15      -74.83     72.84                                   
REMARK 500  5 PHE A  20      148.60   -177.08                                   
REMARK 500  5 PHE A  23       99.42    -58.19                                   
REMARK 500  5 ARG A  28     -167.21    -74.72                                   
REMARK 500  5 TYR A  34      128.73    -37.60                                   
REMARK 500  5 THR A  44       13.81   -140.93                                   
REMARK 500  5 ILE A  56     -106.88    -74.18                                   
REMARK 500  5 TYR A  57      147.19    179.22                                   
REMARK 500  5 CYS A  68     -177.51    166.80                                   
REMARK 500  5 THR A  75      -66.37   -137.90                                   
REMARK 500  5 GLU A  76       71.79   -170.78                                   
REMARK 500  5 LYS A  77      141.79     63.92                                   
REMARK 500  5 ASN A  78      -54.28   -179.59                                   
REMARK 500  5 ASN A  97      124.99   -176.75                                   
REMARK 500  5 THR A 112      -45.10   -146.60                                   
REMARK 500  5 LEU A 122      179.29    -52.68                                   
REMARK 500  5 LYS A 138      -33.84    -38.86                                   
REMARK 500  5 ILE A 142      -82.29    -51.22                                   
REMARK 500  5 GLU A 143      -70.22    -39.43                                   
REMARK 500  5 SER A 147      116.28     63.52                                   
REMARK 500  6 SER A   3      150.71     70.84                                   
REMARK 500  6 SER A   5     -157.66     50.65                                   
REMARK 500  6 GLN A   8      -84.88     62.94                                   
REMARK 500  6 ARG A  10      -54.07   -132.02                                   
REMARK 500  6 LEU A  11       94.51     52.47                                   
REMARK 500  6 THR A  14       95.62     50.69                                   
REMARK 500  6 TYR A  19      -50.32   -127.75                                   
REMARK 500  6 PHE A  23      105.75    -51.93                                   
REMARK 500  6 ARG A  28     -168.87    -56.17                                   
REMARK 500  6 SER A  29      -74.89    -45.71                                   
REMARK 500  6 TYR A  31      -82.54     57.71                                   
REMARK 500  6 ARG A  32        0.33     86.64                                   
REMARK 500  6 TRP A  38       86.04    -63.69                                   
REMARK 500  6 THR A  44       22.23   -147.36                                   
REMARK 500  6 CYS A  68     -172.50    172.11                                   
REMARK 500  6 SER A  74      -60.99   -173.84                                   
REMARK 500  6 ASN A  78      -55.00   -120.77                                   
REMARK 500  6 VAL A 111       30.32    -90.64                                   
REMARK 500  6 THR A 112      -45.36   -146.09                                   
REMARK 500  6 LEU A 122      159.98    -40.66                                   
REMARK 500  6 LEU A 134      -72.04    -60.29                                   
REMARK 500  6 ILE A 142      -83.98    -48.58                                   
REMARK 500  6 SER A 144      149.21     76.77                                   
REMARK 500  6 SER A 148      -64.83     72.07                                   
REMARK 500  7 SER A   3      149.13   -171.99                                   
REMARK 500  7 SER A   5      117.18   -172.44                                   
REMARK 500  7 SER A   6      133.00   -178.52                                   
REMARK 500  7 GLU A   9      -57.83   -139.04                                   
REMARK 500  7 ARG A  10      103.08     54.26                                   
REMARK 500  7 LYS A  12       69.87     64.86                                   
REMARK 500  7 ALA A  15       54.91    -93.54                                   
REMARK 500  7 PHE A  23       98.71    -54.22                                   
REMARK 500  7 ARG A  28      175.16    -55.91                                   
REMARK 500  7 GLU A  33      160.47    179.24                                   
REMARK 500  7 TRP A  38       85.02    -62.61                                   
REMARK 500  7 CYS A  68     -179.67    174.51                                   
REMARK 500  7 SER A  74      -52.28   -174.26                                   
REMARK 500  7 GLU A  76       90.95    -54.12                                   
REMARK 500  7 LYS A  77       85.98     65.84                                   
REMARK 500  7 CYS A  79      162.13     68.73                                   
REMARK 500  7 THR A  95     -166.14   -112.73                                   
REMARK 500  7 SER A 100      -35.89    -39.09                                   
REMARK 500  7 THR A 112      -46.41   -136.88                                   
REMARK 500  7 LEU A 122      160.94    -44.82                                   
REMARK 500  7 ILE A 142      -87.46    -52.85                                   
REMARK 500  7 SER A 144       90.71     49.63                                   
REMARK 500  7 SER A 147       85.35     51.17                                   
REMARK 500  7 SER A 148       95.99     52.77                                   
REMARK 500  8 GLU A   9      125.52   -170.87                                   
REMARK 500  8 LYS A  12       58.62   -161.47                                   
REMARK 500  8 THR A  14      118.34   -160.59                                   
REMARK 500  8 ALA A  15      -74.28     71.41                                   
REMARK 500  8 ARG A  28     -172.57     43.67                                   
REMARK 500  8 ARG A  32      -40.07     86.83                                   
REMARK 500  8 TRP A  38       89.28    -62.51                                   
REMARK 500  8 THR A  44       16.96   -140.18                                   
REMARK 500  8 ASP A  51      -67.75   -141.13                                   
REMARK 500  8 LYS A  52      -62.28   -172.32                                   
REMARK 500  8 TYR A  57      135.52    -37.49                                   
REMARK 500  8 CYS A  68     -178.72    175.85                                   
REMARK 500  8 THR A  75      -63.64   -141.74                                   
REMARK 500  8 GLU A  76       81.86   -171.97                                   
REMARK 500  8 LYS A  77       73.54     43.73                                   
REMARK 500  8 ASN A  78      -60.51   -126.65                                   
REMARK 500  8 ASN A  97      117.21    174.23                                   
REMARK 500  8 GLU A  99      -73.06    -48.70                                   
REMARK 500  8 THR A 112      -46.68   -144.80                                   
REMARK 500  8 ILE A 142      -77.72    -63.88                                   
REMARK 500  8 GLU A 143      -75.87    -52.51                                   
REMARK 500  8 SER A 147      -58.73   -133.75                                   
REMARK 500  8 SER A 148       97.91     52.25                                   
REMARK 500  9 SER A   3      -61.29   -125.96                                   
REMARK 500  9 SER A   5      117.98     63.29                                   
REMARK 500  9 GLU A   9       78.78     41.02                                   
REMARK 500  9 LEU A  16       99.45    -38.90                                   
REMARK 500  9 PHE A  23      103.53    -41.65                                   
REMARK 500  9 TYR A  31      -44.55   -135.21                                   
REMARK 500  9 ARG A  32      -51.06     80.32                                   
REMARK 500  9 TRP A  38       95.81    -65.68                                   
REMARK 500  9 THR A  44       32.81   -151.48                                   
REMARK 500  9 CYS A  68     -178.10    175.46                                   
REMARK 500  9 SER A  74      177.67     68.63                                   
REMARK 500  9 THR A  75       95.35    -61.91                                   
REMARK 500  9 GLU A  76       69.13     39.11                                   
REMARK 500  9 LYS A  77       85.48     41.90                                   
REMARK 500  9 CYS A  79      160.16     63.06                                   
REMARK 500  9 THR A 112      -51.41   -122.39                                   
REMARK 500  9 SER A 115     -154.17   -138.79                                   
REMARK 500  9 ILE A 142      -82.07    -45.89                                   
REMARK 500  9 SER A 144      -61.02   -158.37                                   
REMARK 500 10 SER A   2      135.39     65.51                                   
REMARK 500 10 LYS A  12     -164.51     46.79                                   
REMARK 500 10 THR A  14      -54.10   -156.11                                   
REMARK 500 10 LEU A  16      103.55    -40.30                                   
REMARK 500 10 PHE A  23       96.47    -38.00                                   
REMARK 500 10 ARG A  28      173.18    -46.92                                   
REMARK 500 10 ARG A  32      -31.07    -38.18                                   
REMARK 500 10 TRP A  38       92.88    -60.40                                   
REMARK 500 10 THR A  44       20.17   -146.47                                   
REMARK 500 10 CYS A  68     -179.16   -179.04                                   
REMARK 500 10 ASN A  73      105.67     65.08                                   
REMARK 500 10 THR A  75       92.42     50.56                                   
REMARK 500 10 GLU A  76      126.73    178.18                                   
REMARK 500 10 LYS A  77      138.70     66.96                                   
REMARK 500 10 LEU A 114       33.09     39.32                                   
REMARK 500 10 LEU A 122      161.06    -43.44                                   
REMARK 500 10 ILE A 142      -78.58    -41.60                                   
REMARK 500 10 SER A 147      101.06   -170.70                                   
REMARK 500 11 SER A   2       85.46     67.08                                   
REMARK 500 11 GLN A   8       74.44   -178.21                                   
REMARK 500 11 THR A  14      -52.84   -157.61                                   
REMARK 500 11 TYR A  19      -70.06   -115.18                                   
REMARK 500 11 GLU A  21      112.83   -173.20                                   
REMARK 500 11 SER A  29       78.95    -69.37                                   
REMARK 500 11 TYR A  31      -46.72   -133.61                                   
REMARK 500 11 ARG A  32      -48.58     84.17                                   
REMARK 500 11 TRP A  38       90.35    -65.87                                   
REMARK 500 11 THR A  44       21.68   -141.72                                   
REMARK 500 11 CYS A  68     -177.68    171.74                                   
REMARK 500 11 ASN A  73      169.74    179.60                                   
REMARK 500 11 THR A  75      -80.68     62.75                                   
REMARK 500 11 CYS A  79      156.64     60.86                                   
REMARK 500 11 THR A 112      -45.93   -142.92                                   
REMARK 500 11 SER A 115     -158.48   -145.45                                   
REMARK 500 11 LEU A 122      177.50    -51.10                                   
REMARK 500 11 ILE A 142      -82.03    -45.90                                   
REMARK 500 12 GLN A   8      103.54     54.28                                   
REMARK 500 12 LEU A  11      157.71     59.02                                   
REMARK 500 12 ALA A  15      -56.15   -153.68                                   
REMARK 500 12 LEU A  16      129.89     63.52                                   
REMARK 500 12 PHE A  20      140.76   -170.86                                   
REMARK 500 12 ARG A  28     -155.23     36.08                                   
REMARK 500 12 TYR A  31      171.97     52.45                                   
REMARK 500 12 GLU A  33      146.77   -178.38                                   
REMARK 500 12 TRP A  38       84.85    -63.78                                   
REMARK 500 12 THR A  44       16.76   -142.24                                   
REMARK 500 12 CYS A  68     -178.43    170.61                                   
REMARK 500 12 SER A  74       70.69    176.11                                   
REMARK 500 12 GLU A 103      -70.08    -55.56                                   
REMARK 500 12 THR A 112      -45.42   -144.16                                   
REMARK 500 12 LEU A 122     -176.82    -52.08                                   
REMARK 500 12 ILE A 142      -80.16    -63.19                                   
REMARK 500 12 SER A 147      -59.05   -129.81                                   
REMARK 500 13 SER A   3      141.64   -178.89                                   
REMARK 500 13 SER A   5      138.40     61.79                                   
REMARK 500 13 PHE A  23      106.14    -46.71                                   
REMARK 500 13 LYS A  27       56.12   -149.51                                   
REMARK 500 13 SER A  29      -52.36   -169.87                                   
REMARK 500 13 TYR A  31      -73.12   -150.59                                   
REMARK 500 13 ARG A  32      -47.03     82.50                                   
REMARK 500 13 TYR A  34      149.25     58.79                                   
REMARK 500 13 TRP A  38       95.74    -67.43                                   
REMARK 500 13 LEU A  66      132.50    -39.36                                   
REMARK 500 13 CYS A  68     -179.90    171.56                                   
REMARK 500 13 ASN A  73      140.29    176.03                                   
REMARK 500 13 THR A  75       70.28     50.93                                   
REMARK 500 13 GLU A  76       52.29   -175.31                                   
REMARK 500 13 LYS A  77      145.98    -38.78                                   
REMARK 500 13 ASN A  78      -50.65   -169.08                                   
REMARK 500 13 THR A 112      -46.38   -144.35                                   
REMARK 500 13 SER A 115     -156.73   -144.03                                   
REMARK 500 13 LEU A 122      159.79    -42.93                                   
REMARK 500 13 LEU A 134      -72.36    -60.38                                   
REMARK 500 13 ILE A 142      -79.46    -43.62                                   
REMARK 500 13 GLU A 143      -75.42    -41.60                                   
REMARK 500 13 SER A 148       84.18     64.02                                   
REMARK 500 14 SER A   2       89.24     46.02                                   
REMARK 500 14 SER A   5      114.69   -177.16                                   
REMARK 500 14 SER A   6      123.47   -174.96                                   
REMARK 500 14 GLU A   9      151.70     64.16                                   
REMARK 500 14 LEU A  11      124.65     64.93                                   
REMARK 500 14 ALA A  15      151.78     67.26                                   
REMARK 500 14 TYR A  19      -55.22   -130.55                                   
REMARK 500 14 PHE A  23       98.89    -44.64                                   
REMARK 500 14 ARG A  28     -175.01    -64.21                                   
REMARK 500 14 TYR A  31      -43.65   -159.93                                   
REMARK 500 14 ARG A  32      -51.88     75.82                                   
REMARK 500 14 TYR A  34      143.37    -37.87                                   
REMARK 500 14 TRP A  38       86.62    -65.62                                   
REMARK 500 14 THR A  44       17.47   -145.20                                   
REMARK 500 14 CYS A  68     -179.01    172.17                                   
REMARK 500 14 GLU A  76      169.00     58.75                                   
REMARK 500 14 LYS A  77      104.19     56.82                                   
REMARK 500 14 GLU A  99      -74.63    -42.91                                   
REMARK 500 14 VAL A 111       30.34    -92.40                                   
REMARK 500 14 THR A 112      -47.53   -146.20                                   
REMARK 500 14 LEU A 122      176.72    -51.70                                   
REMARK 500 14 ARG A 141      -36.01    -38.89                                   
REMARK 500 14 ILE A 142      -80.78    -45.26                                   
REMARK 500 14 SER A 144      -59.70   -139.75                                   
REMARK 500 15 GLN A   8       92.62   -169.29                                   
REMARK 500 15 GLU A   9      -46.42   -173.73                                   
REMARK 500 15 ARG A  10      100.36     54.30                                   
REMARK 500 15 SER A  29       99.70    -62.48                                   
REMARK 500 15 ARG A  32      -51.31     80.90                                   
REMARK 500 15 TYR A  34      130.58    -37.84                                   
REMARK 500 15 TRP A  38       92.47    -64.07                                   
REMARK 500 15 ASP A  51      -51.20   -157.58                                   
REMARK 500 15 LYS A  52      -36.37    176.34                                   
REMARK 500 15 ILE A  55      -65.53     67.81                                   
REMARK 500 15 ILE A  56     -113.36    -92.32                                   
REMARK 500 15 CYS A  68     -178.77   -175.80                                   
REMARK 500 15 ASN A  73      152.09    -42.54                                   
REMARK 500 15 GLU A  76       77.68   -176.28                                   
REMARK 500 15 LYS A  77       79.11     44.70                                   
REMARK 500 15 ASN A  78      -55.67   -121.61                                   
REMARK 500 15 VAL A 111       30.56    -91.64                                   
REMARK 500 15 THR A 112      -44.88   -147.99                                   
REMARK 500 15 LEU A 114       29.54     40.04                                   
REMARK 500 15 ASN A 119       48.94    -85.90                                   
REMARK 500 15 LEU A 122      171.00    -45.94                                   
REMARK 500 15 LEU A 134      -73.27    -54.98                                   
REMARK 500 15 ILE A 142      -77.75    -41.53                                   
REMARK 500 15 SER A 148      -58.09   -124.50                                   
REMARK 500 16 GLN A   8       80.90     56.56                                   
REMARK 500 16 THR A  14      141.48   -176.51                                   
REMARK 500 16 ALA A  15      -75.64   -165.23                                   
REMARK 500 16 PHE A  23      104.22    -54.20                                   
REMARK 500 16 ARG A  28      170.50    -57.09                                   
REMARK 500 16 SER A  29       77.94    -65.26                                   
REMARK 500 16 TYR A  31      -58.12   -146.60                                   
REMARK 500 16 ARG A  32      -40.35     87.27                                   
REMARK 500 16 TRP A  38       85.35    -68.46                                   
REMARK 500 16 THR A  44       21.14   -147.33                                   
REMARK 500 16 ASP A  51     -164.14    178.44                                   
REMARK 500 16 SER A  54      118.34    -38.85                                   
REMARK 500 16 ILE A  56      -81.91   -107.66                                   
REMARK 500 16 TYR A  57      148.68    179.36                                   
REMARK 500 16 ASP A  59      169.26    174.62                                   
REMARK 500 16 CYS A  68     -177.32    169.66                                   
REMARK 500 16 THR A  70     -169.35   -117.08                                   
REMARK 500 16 SER A  74      -71.19   -179.95                                   
REMARK 500 16 LYS A  77      132.44     68.09                                   
REMARK 500 16 GLU A  96       46.00   -100.23                                   
REMARK 500 16 ASN A  97      155.93    168.14                                   
REMARK 500 16 THR A 112      -45.59   -142.87                                   
REMARK 500 16 SER A 115     -157.45   -141.67                                   
REMARK 500 16 LEU A 122      158.90    -40.02                                   
REMARK 500 16 LEU A 134      -70.18    -59.23                                   
REMARK 500 16 ILE A 142      -82.35    -51.19                                   
REMARK 500 17 SER A   5      102.26   -173.27                                   
REMARK 500 17 SER A   6       94.03   -179.59                                   
REMARK 500 17 GLN A   8      -58.40   -141.09                                   
REMARK 500 17 LEU A  11      -75.13     66.00                                   
REMARK 500 17 ILE A  13      -77.76     65.52                                   
REMARK 500 17 THR A  14       90.40     60.93                                   
REMARK 500 17 PHE A  23      102.74    -41.04                                   
REMARK 500 17 ARG A  28      173.57    -48.08                                   
REMARK 500 17 ARG A  32      -30.65    -38.84                                   
REMARK 500 17 GLU A  33      170.33    174.07                                   
REMARK 500 17 TRP A  38       89.22    -61.42                                   
REMARK 500 17 THR A  44       23.78   -144.65                                   
REMARK 500 17 ASP A  51      -70.93   -143.54                                   
REMARK 500 17 LYS A  52      -59.03   -173.12                                   
REMARK 500 17 TYR A  57      133.95    -37.42                                   
REMARK 500 17 CYS A  68     -173.82    175.44                                   
REMARK 500 17 SER A  74      -55.19    169.45                                   
REMARK 500 17 GLU A  76       98.70    -43.80                                   
REMARK 500 17 CYS A  79      157.77     61.97                                   
REMARK 500 17 THR A  95     -165.50   -114.01                                   
REMARK 500 17 GLU A  96      -68.65    -95.86                                   
REMARK 500 17 VAL A 111       30.26    -88.68                                   
REMARK 500 17 THR A 112      -45.43   -145.64                                   
REMARK 500 17 LEU A 122      159.61    -40.12                                   
REMARK 500 17 LEU A 134      -74.93    -61.59                                   
REMARK 500 17 ILE A 142      -79.02    -42.21                                   
REMARK 500 17 SER A 148      -58.20   -142.44                                   
REMARK 500 18 SER A   2      -50.65   -130.61                                   
REMARK 500 18 SER A   3      -87.79     64.41                                   
REMARK 500 18 SER A   5      112.05   -164.51                                   
REMARK 500 18 GLN A   8       94.56   -162.37                                   
REMARK 500 18 ARG A  10       98.74    -62.43                                   
REMARK 500 18 LEU A  11      163.04    -48.12                                   
REMARK 500 18 ALA A  15      -68.51     82.78                                   
REMARK 500 18 ARG A  28     -156.35    -67.45                                   
REMARK 500 18 ARG A  32      -54.35     69.55                                   
REMARK 500 18 TYR A  34      133.63    -39.39                                   
REMARK 500 18 TRP A  38       88.79    -66.42                                   
REMARK 500 18 ASP A  51      -71.92   -154.89                                   
REMARK 500 18 LYS A  52      -61.73   -171.71                                   
REMARK 500 18 TYR A  57      136.69    -37.91                                   
REMARK 500 18 ILE A  63       53.30   -142.97                                   
REMARK 500 18 CYS A  68     -171.21    174.13                                   
REMARK 500 18 SER A  74      -57.48   -178.81                                   
REMARK 500 18 THR A  75      150.31     61.77                                   
REMARK 500 18 GLU A  76      159.07     64.12                                   
REMARK 500 18 ASN A  78      168.98    178.59                                   
REMARK 500 18 CYS A  79      159.25    -46.53                                   
REMARK 500 18 GLU A  96       44.84   -102.66                                   
REMARK 500 18 ASN A  97      165.02    176.31                                   
REMARK 500 18 ARG A 105      -33.79    -38.11                                   
REMARK 500 18 THR A 112      -44.18   -144.76                                   
REMARK 500 18 LEU A 114       34.23     37.32                                   
REMARK 500 18 LEU A 122     -177.88    -50.20                                   
REMARK 500 18 LEU A 134      -72.24    -52.12                                   
REMARK 500 18 ILE A 142      -76.36    -39.68                                   
REMARK 500 18 SER A 144      138.30   -179.53                                   
REMARK 500 18 SER A 148      170.47    -59.61                                   
REMARK 500 19 SER A   2      146.37   -178.63                                   
REMARK 500 19 SER A   3       96.08   -178.52                                   
REMARK 500 19 THR A  14       83.03     43.22                                   
REMARK 500 19 PHE A  23       98.50    -41.87                                   
REMARK 500 19 ARG A  28      -91.03     40.35                                   
REMARK 500 19 SER A  29       78.60   -175.89                                   
REMARK 500 19 ARG A  32      -52.65     76.48                                   
REMARK 500 19 TYR A  34      150.97     58.37                                   
REMARK 500 19 TRP A  38       86.78    -61.60                                   
REMARK 500 19 THR A  44       60.57     32.92                                   
REMARK 500 19 THR A  45      106.18   -167.61                                   
REMARK 500 19 ASP A  51      -81.00   -176.81                                   
REMARK 500 19 LYS A  52      -59.43   -161.84                                   
REMARK 500 19 CYS A  68     -178.75    168.98                                   
REMARK 500 19 THR A  75      -59.51   -122.54                                   
REMARK 500 19 LYS A  77      109.53     60.15                                   
REMARK 500 19 GLU A  96      -62.06   -102.05                                   
REMARK 500 19 SER A 115     -158.12   -138.33                                   
REMARK 500 19 LEU A 122      160.62    -47.35                                   
REMARK 500 19 ILE A 142      -82.23    -45.94                                   
REMARK 500 19 SER A 144      155.81     69.18                                   
REMARK 500 19 SER A 147      -56.02    179.37                                   
REMARK 500 20 ARG A  10       91.06     50.62                                   
REMARK 500 20 THR A  14      -54.55   -145.92                                   
REMARK 500 20 LEU A  18      159.41     55.70                                   
REMARK 500 20 TYR A  19      -41.46   -144.58                                   
REMARK 500 20 GLU A  21      109.90   -161.02                                   
REMARK 500 20 SER A  29       83.62    -69.38                                   
REMARK 500 20 TYR A  31      -44.91   -134.57                                   
REMARK 500 20 ARG A  32      -49.87     82.18                                   
REMARK 500 20 TRP A  38       94.81    -64.06                                   
REMARK 500 20 ASP A  51      -65.53   -144.99                                   
REMARK 500 20 LYS A  52      -54.74   -176.53                                   
REMARK 500 20 TYR A  57      133.64    -37.57                                   
REMARK 500 20 CYS A  68     -179.55    172.66                                   
REMARK 500 20 SER A  74      115.94   -164.26                                   
REMARK 500 20 GLU A  76      162.11     59.15                                   
REMARK 500 20 LYS A  77      104.46    -49.84                                   
REMARK 500 20 GLU A  96      -75.16    -83.56                                   
REMARK 500 20 THR A 112      -45.71   -143.73                                   
REMARK 500 20 ASN A 119       40.89    -90.74                                   
REMARK 500 20 LEU A 122      161.72    -42.92                                   
REMARK 500 20 ILE A 142      -78.74    -42.38                                   
REMARK 500 20 SER A 144      170.37     67.46                                   
REMARK 500 20 SER A 147      -57.19   -141.73                                   
REMARK 500 20 SER A 148      154.42     63.44                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650 HELIX                                                                
REMARK 650 DETERMINATION METHOD: AUTHOR DETERMINED                              
REMARK 700                                                                      
REMARK 700 SHEET                                                                
REMARK 700 DETERMINATION METHOD: AUTHOR DETERMINED                              
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSS001002916.1   RELATED DB: TARGETDB                    
DBREF  1X1F A    8   143  UNP    Q9ULZ2   STAP1_HUMAN     16    151             
SEQADV 1X1F GLY A    1  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A    2  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A    3  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F GLY A    4  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A    5  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A    6  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F GLY A    7  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A  144  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F GLY A  145  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F PRO A  146  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A  147  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F SER A  148  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQADV 1X1F GLY A  149  UNP  Q9ULZ2              CLONING ARTIFACT               
SEQRES   1 A  149  GLY SER SER GLY SER SER GLY GLN GLU ARG LEU LYS ILE          
SEQRES   2 A  149  THR ALA LEU PRO LEU TYR PHE GLU GLY PHE LEU LEU ILE          
SEQRES   3 A  149  LYS ARG SER GLY TYR ARG GLU TYR GLU HIS TYR TRP THR          
SEQRES   4 A  149  GLU LEU ARG GLY THR THR LEU PHE PHE TYR THR ASP LYS          
SEQRES   5 A  149  LYS SER ILE ILE TYR VAL ASP LYS LEU ASP ILE VAL ASP          
SEQRES   6 A  149  LEU THR CYS LEU THR GLU GLN ASN SER THR GLU LYS ASN          
SEQRES   7 A  149  CYS ALA LYS PHE THR LEU VAL LEU PRO LYS GLU GLU VAL          
SEQRES   8 A  149  GLN LEU LYS THR GLU ASN THR GLU SER GLY GLU GLU TRP          
SEQRES   9 A  149  ARG GLY PHE ILE LEU THR VAL THR GLU LEU SER VAL PRO          
SEQRES  10 A  149  GLN ASN VAL SER LEU LEU PRO GLY GLN VAL ILE LYS LEU          
SEQRES  11 A  149  HIS GLU VAL LEU GLU ARG GLU LYS LYS ARG ARG ILE GLU          
SEQRES  12 A  149  SER GLY PRO SER SER GLY                                      
HELIX    1   1 THR A   98  GLU A  113  1                                  16    
HELIX    2   2 PRO A  124  ILE A  142  1                                  19    
SHEET    1   A 7 ASP A  59  ASP A  62  0                                        
SHEET    2   A 7 THR A  45  TYR A  49 -1  N  PHE A  48   O  ASP A  59           
SHEET    3   A 7 GLU A  35  ARG A  42 -1  N  ARG A  42   O  THR A  45           
SHEET    4   A 7 LEU A  18  LYS A  27 -1  N  ILE A  26   O  GLU A  35           
SHEET    5   A 7 VAL A  91  LYS A  94 -1  O  GLN A  92   N  LYS A  27           
SHEET    6   A 7 LYS A  81  VAL A  85 -1  N  LEU A  84   O  VAL A  91           
SHEET    7   A 7 CYS A  68  GLU A  71 -1  N  CYS A  68   O  VAL A  85           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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