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Database: PDB
Entry: 1X5T
LinkDB: 1X5T
Original site: 1X5T 
HEADER    RNA BINDING PROTEIN                     16-MAY-05   1X5T              
TITLE     SOLUTION STRUCTURE OF THE SECOND RRM DOMAIN IN SPLICING               
TITLE    2 FACTOR = 3B                                                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SPLICING FACTOR 3B SUBUNIT 4;                              
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: RRM DOMAIN;                                                
COMPND   5 SYNONYM: SPLICEOSOME ASSOCIATED PROTEIN 49, SAP 49, SF3B50,          
COMPND   6 PRE-MRNA SPLICING FACTOR SF3B 49 KDA SUBUNIT;                        
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: SF3B4, SAP49;                                                  
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040517-06;                               
SOURCE   8 OTHER_DETAILS: CELL FREE PROTEIN SYNTHESIS                           
KEYWDS    NMR, STRUCTURE GENOMICS, RRM DOMAIN, STRUCTURAL GENOMICS,             
KEYWDS   2 NPPSFA, NATIONAL PROJECT ON PROTEIN STRUCTURAL AND                   
KEYWDS   3 FUNCTIONAL ANALYSES, RIKEN STRUCTURAL GENOMICS/PROTEOMICS            
KEYWDS   4 INITIATIVE, RSGI, RNA BINDING PROTEIN                                
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    A.SATO,K.KUWASAKO,Y.MUTO,M.INOUE,T.KIGAWA,M.SHIROUZU,                 
AUTHOR   2 T.TERADA,S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS             
AUTHOR   3 INITIATIVE (RSGI)                                                    
REVDAT   2   24-FEB-09 1X5T    1       VERSN                                    
REVDAT   1   16-NOV-05 1X5T    0                                                
JRNL        AUTH   A.SATO,K.KUWASAKO,Y.MUTO,M.INOUE,T.KIGAWA,                   
JRNL        AUTH 2 M.SHIROUZU,T.TERADA,S.YOKOYAMA                               
JRNL        TITL   SOLUTION STRUCTURE OF THE SECOND RRM DOMAIN IN               
JRNL        TITL 2 SPLICING FACTOR = 3B                                         
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT,P.                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1X5T COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 23-MAY-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB024415.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.8MM U-15, 13C; 20MM              
REMARK 210                                   PHOSPHATE BUFFER NA; 100MM         
REMARK 210                                   NACL; 1MM D-DTT; 0.02% NAN3;       
REMARK 210                                   90% H2O, 10% D2O                   
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 700 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 2.6, NMRPIPE               
REMARK 210                                   20031121, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.925, CYANA 2.0.17         
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION, STRUCTURES        
REMARK 210                                   WITH THE LOWEST ENERGY             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   2      137.82   -172.80                                   
REMARK 500  1 THR A  25      -36.81    -39.97                                   
REMARK 500  1 THR A  35      132.34    -38.39                                   
REMARK 500  1 ASP A  43      -71.99    -96.78                                   
REMARK 500  1 SER A  47      121.14   -174.82                                   
REMARK 500  1 ASN A  69       84.04    -64.04                                   
REMARK 500  1 GLN A  71      119.77    -35.46                                   
REMARK 500  1 CYS A  74       91.09    -32.19                                   
REMARK 500  1 ASN A  75       47.57     39.39                                   
REMARK 500  1 ARG A  76      109.83   -170.43                                   
REMARK 500  1 TYR A  82      122.98    -38.16                                   
REMARK 500  2 ASN A  12       49.69     74.10                                   
REMARK 500  2 THR A  25      -38.48    -37.54                                   
REMARK 500  2 PHE A  29      -70.60    -57.51                                   
REMARK 500  2 THR A  35      138.97    -35.42                                   
REMARK 500  2 PRO A  42        1.67    -69.76                                   
REMARK 500  2 ASN A  46      104.26    -43.30                                   
REMARK 500  2 SER A  47      122.87    -35.64                                   
REMARK 500  2 ALA A  67      -60.12    -93.10                                   
REMARK 500  2 ASN A  69       86.93    -69.18                                   
REMARK 500  2 CYS A  74       90.90    -33.69                                   
REMARK 500  2 ARG A  76      106.03   -170.32                                   
REMARK 500  2 TYR A  82      108.14    -45.71                                   
REMARK 500  2 LYS A  85      123.21   -171.68                                   
REMARK 500  2 SER A  94       49.54     34.45                                   
REMARK 500  3 PRO A  15        0.50    -69.75                                   
REMARK 500  3 LEU A  21      -70.17    -48.28                                   
REMARK 500  3 THR A  35      133.41    -37.05                                   
REMARK 500  3 SER A  47      105.48    -48.03                                   
REMARK 500  3 ASN A  69       81.47    -65.99                                   
REMARK 500  3 CYS A  74       90.87    -33.17                                   
REMARK 500  3 ARG A  76      105.58   -170.42                                   
REMARK 500  3 ASP A  87      134.29   -172.41                                   
REMARK 500  4 THR A  35      135.19    -39.72                                   
REMARK 500  4 ASP A  43      -64.36   -100.37                                   
REMARK 500  4 ASN A  69       73.91    -63.98                                   
REMARK 500  4 GLN A  71      127.90    -34.06                                   
REMARK 500  4 CYS A  74       90.96    -32.16                                   
REMARK 500  4 ASN A  75       47.15     39.35                                   
REMARK 500  4 ARG A  76      112.81   -170.60                                   
REMARK 500  4 TYR A  82      127.42    -35.79                                   
REMARK 500  4 LYS A  86       56.34   -112.82                                   
REMARK 500  5 THR A  35      136.67    -36.52                                   
REMARK 500  5 THR A  44      -74.49    -77.08                                   
REMARK 500  5 SER A  47      107.32    -47.60                                   
REMARK 500  5 ALA A  67      -60.66    -96.59                                   
REMARK 500  5 ASN A  69       96.54    -56.69                                   
REMARK 500  5 CYS A  74       90.39    -31.71                                   
REMARK 500  5 ASN A  75       48.08     39.81                                   
REMARK 500  5 ARG A  76      106.66   -170.93                                   
REMARK 500  5 PRO A  77       99.56    -69.79                                   
REMARK 500  5 TYR A  82      100.70    -51.27                                   
REMARK 500  5 ALA A  83      131.62    -38.15                                   
REMARK 500  6 THR A  35      132.56    -39.95                                   
REMARK 500  6 ASN A  69       83.37    -60.48                                   
REMARK 500  6 GLN A  71      122.03    -39.33                                   
REMARK 500  6 CYS A  74       91.34    -32.65                                   
REMARK 500  6 ARG A  76      109.33   -170.11                                   
REMARK 500  6 LYS A  85      119.20    -39.27                                   
REMARK 500  6 SER A  88      -56.20   -126.70                                   
REMARK 500  7 SER A   2     -178.01    -55.74                                   
REMARK 500  7 SER A   5       57.40   -108.02                                   
REMARK 500  7 ASN A  12       54.97     74.12                                   
REMARK 500  7 THR A  25      -36.55    -37.94                                   
REMARK 500  7 THR A  35      131.26    -36.48                                   
REMARK 500  7 MET A  39       96.46    -67.54                                   
REMARK 500  7 ARG A  40      -75.69    -67.95                                   
REMARK 500  7 ASP A  43      -75.28   -127.29                                   
REMARK 500  7 ASN A  46      -72.15    -97.96                                   
REMARK 500  7 SER A  47      121.47   -173.97                                   
REMARK 500  7 ILE A  65      -19.73    -49.06                                   
REMARK 500  7 ALA A  67      -61.05   -103.19                                   
REMARK 500  7 ASN A  69       87.18    -59.79                                   
REMARK 500  7 GLN A  71      122.07    -38.97                                   
REMARK 500  7 CYS A  74       91.08    -30.28                                   
REMARK 500  7 ASN A  75       49.30     37.94                                   
REMARK 500  7 ARG A  76      110.65   -170.64                                   
REMARK 500  8 SER A   2      -73.57    -51.86                                   
REMARK 500  8 THR A  35      133.27    -34.26                                   
REMARK 500  8 PRO A  42        2.72    -69.76                                   
REMARK 500  8 ASN A  69       79.69    -57.65                                   
REMARK 500  8 CYS A  74       90.57    -30.94                                   
REMARK 500  8 ASN A  75       49.34     39.07                                   
REMARK 500  8 ARG A  76      106.78   -171.26                                   
REMARK 500  8 ALA A  83       96.24    -36.45                                   
REMARK 500  9 SER A   3      128.13    -39.07                                   
REMARK 500  9 SER A   6       99.97    -48.19                                   
REMARK 500  9 ASN A  12       49.24     72.04                                   
REMARK 500  9 THR A  35      136.34    -35.39                                   
REMARK 500  9 PRO A  42        3.36    -69.77                                   
REMARK 500  9 ASP A  43      -73.36   -115.63                                   
REMARK 500  9 SER A  47      147.97   -173.34                                   
REMARK 500  9 ASN A  69       78.57    -58.41                                   
REMARK 500  9 GLN A  71      134.99    -35.46                                   
REMARK 500  9 CYS A  74       91.23    -28.21                                   
REMARK 500  9 ASN A  75       50.05     35.18                                   
REMARK 500  9 ARG A  76      112.19   -171.08                                   
REMARK 500 10 ASN A  12       47.09     71.25                                   
REMARK 500 10 PRO A  15        1.93    -69.80                                   
REMARK 500 10 THR A  25      -35.57    -36.99                                   
REMARK 500 10 THR A  35      131.90    -36.01                                   
REMARK 500 10 ASP A  43      -63.19    -90.84                                   
REMARK 500 10 SER A  47      103.67    -58.99                                   
REMARK 500 10 ALA A  64      -72.19    -58.64                                   
REMARK 500 10 ILE A  65      -26.50    -39.84                                   
REMARK 500 10 ASN A  69       88.13    -58.27                                   
REMARK 500 10 GLN A  71      121.50    -34.19                                   
REMARK 500 10 CYS A  74       90.74    -33.09                                   
REMARK 500 10 ARG A  76      111.07   -169.79                                   
REMARK 500 11 ASN A  12       50.67     71.38                                   
REMARK 500 11 THR A  25      -32.41    -39.61                                   
REMARK 500 11 SER A  27      -29.91    -39.71                                   
REMARK 500 11 THR A  35      137.66    -39.44                                   
REMARK 500 11 ASP A  41      109.07    -50.31                                   
REMARK 500 11 ASP A  43      -70.93    -95.94                                   
REMARK 500 11 SER A  47      106.62    -38.62                                   
REMARK 500 11 ILE A  65      -19.28    -48.00                                   
REMARK 500 11 ASN A  69       96.98    -51.50                                   
REMARK 500 11 GLN A  71      140.68    -34.96                                   
REMARK 500 11 CYS A  74       91.76    -29.52                                   
REMARK 500 11 ASN A  75       48.19     35.92                                   
REMARK 500 11 ARG A  76      105.88   -171.79                                   
REMARK 500 11 LYS A  85      -32.47    -36.36                                   
REMARK 500 11 SER A  91      -34.74   -131.44                                   
REMARK 500 12 ASN A  12       47.59     73.91                                   
REMARK 500 12 THR A  25      -36.74    -39.19                                   
REMARK 500 12 THR A  35      137.76    -35.08                                   
REMARK 500 12 ILE A  65      -19.52    -49.11                                   
REMARK 500 12 ALA A  67      -61.00   -105.15                                   
REMARK 500 12 ASN A  69       75.28    -61.63                                   
REMARK 500 12 GLN A  71      130.42    -38.21                                   
REMARK 500 12 CYS A  74       91.24    -29.87                                   
REMARK 500 12 ASN A  75       49.16     36.93                                   
REMARK 500 12 ARG A  76      109.94   -170.94                                   
REMARK 500 12 SER A  91      135.21   -171.72                                   
REMARK 500 13 SER A   5       44.56   -104.20                                   
REMARK 500 13 SER A   6       43.06    -91.91                                   
REMARK 500 13 ASN A  12       54.05     74.46                                   
REMARK 500 13 THR A  25      -38.80    -38.51                                   
REMARK 500 13 THR A  35      133.11    -36.78                                   
REMARK 500 13 ASP A  41      109.42    -47.11                                   
REMARK 500 13 ALA A  67      -60.81    -96.58                                   
REMARK 500 13 ASN A  69       82.03    -56.88                                   
REMARK 500 13 CYS A  74       90.28    -30.61                                   
REMARK 500 13 ASN A  75       49.35     38.87                                   
REMARK 500 13 ARG A  76      107.29   -171.14                                   
REMARK 500 13 PRO A  77       99.21    -69.80                                   
REMARK 500 13 PHE A  84      114.98    -39.68                                   
REMARK 500 14 SER A   2       89.98    -60.51                                   
REMARK 500 14 THR A  35      133.32    -36.04                                   
REMARK 500 14 SER A  47      106.32    -44.57                                   
REMARK 500 14 ALA A  67      -60.37   -101.95                                   
REMARK 500 14 ASN A  69       74.06    -63.82                                   
REMARK 500 14 CYS A  74       91.41    -28.67                                   
REMARK 500 14 ASN A  75       49.70     35.58                                   
REMARK 500 14 ARG A  76      112.75   -171.40                                   
REMARK 500 15 ASN A  12       35.03     72.55                                   
REMARK 500 15 THR A  35      135.48    -38.06                                   
REMARK 500 15 ASP A  41      100.71    -35.29                                   
REMARK 500 15 ILE A  65      -19.80    -47.60                                   
REMARK 500 15 ALA A  67      -60.34   -101.88                                   
REMARK 500 15 ASN A  69      102.70    -59.13                                   
REMARK 500 15 CYS A  74       91.85    -31.36                                   
REMARK 500 15 ASN A  75       46.51     38.29                                   
REMARK 500 15 ARG A  76      109.68   -171.35                                   
REMARK 500 15 ALA A  83       89.35    -51.40                                   
REMARK 500 15 LYS A  85      -74.27   -128.29                                   
REMARK 500 15 SER A  94       99.07    -48.94                                   
REMARK 500 16 SER A   3      -62.95   -128.10                                   
REMARK 500 16 ASN A  12       54.52     74.96                                   
REMARK 500 16 SER A  27      -39.61    -34.35                                   
REMARK 500 16 THR A  35      137.17    -33.52                                   
REMARK 500 16 PRO A  42        0.96    -69.76                                   
REMARK 500 16 ASN A  46      -63.02   -134.80                                   
REMARK 500 16 SER A  47      105.43   -172.61                                   
REMARK 500 16 ILE A  65      -19.98    -49.10                                   
REMARK 500 16 ALA A  67      -61.15    -98.96                                   
REMARK 500 16 ASN A  69       91.39    -55.12                                   
REMARK 500 16 GLN A  71      132.30    -33.59                                   
REMARK 500 16 CYS A  74       92.07    -27.65                                   
REMARK 500 16 ASN A  75       50.55     35.83                                   
REMARK 500 16 ARG A  76      112.10   -172.46                                   
REMARK 500 16 SER A  88       93.86    -61.88                                   
REMARK 500 17 SER A   2       40.90    -88.98                                   
REMARK 500 17 SER A   5       48.68    -85.56                                   
REMARK 500 17 ASN A  12       45.44     72.63                                   
REMARK 500 17 THR A  35      134.61    -39.64                                   
REMARK 500 17 ALA A  67      -61.20    -98.57                                   
REMARK 500 17 ASN A  69       86.37    -63.80                                   
REMARK 500 17 CYS A  74       89.94    -26.52                                   
REMARK 500 17 ASN A  75       52.21     35.52                                   
REMARK 500 17 ARG A  76      109.46   -171.09                                   
REMARK 500 17 PRO A  77       95.63    -69.77                                   
REMARK 500 17 TYR A  82      107.93    -58.66                                   
REMARK 500 17 LYS A  86       84.15    -62.17                                   
REMARK 500 17 ASP A  87       42.15   -106.86                                   
REMARK 500 17 SER A  91      -56.07   -129.97                                   
REMARK 500 17 PRO A  93       95.06    -69.75                                   
REMARK 500 17 SER A  94      109.52    -38.55                                   
REMARK 500 17 SER A  95      165.64    -42.64                                   
REMARK 500 18 ASN A  12       45.27     71.40                                   
REMARK 500 18 LEU A  21      -70.31    -51.08                                   
REMARK 500 18 THR A  35      138.30    -37.29                                   
REMARK 500 18 ASN A  69       84.82    -61.08                                   
REMARK 500 18 GLN A  71      125.86    -38.17                                   
REMARK 500 18 CYS A  74       90.61    -26.00                                   
REMARK 500 18 ASN A  75       52.83     39.24                                   
REMARK 500 18 ARG A  76      109.88   -175.02                                   
REMARK 500 19 SER A   2      -70.74    -44.63                                   
REMARK 500 19 ASN A  12       54.98     73.66                                   
REMARK 500 19 THR A  35      131.80    -37.71                                   
REMARK 500 19 ASP A  41      117.12    -38.17                                   
REMARK 500 19 ALA A  67      -60.76   -104.83                                   
REMARK 500 19 ASN A  69       76.47    -66.65                                   
REMARK 500 19 CYS A  74       90.69    -27.00                                   
REMARK 500 19 ASN A  75       50.59     34.63                                   
REMARK 500 19 ARG A  76      109.54   -172.46                                   
REMARK 500 19 TYR A  82      108.44    -50.01                                   
REMARK 500 19 LYS A  86       94.55    -63.40                                   
REMARK 500 19 LYS A  89       99.51    -66.10                                   
REMARK 500 19 SER A  94       35.97     39.75                                   
REMARK 500 20 SER A   3       40.01     37.39                                   
REMARK 500 20 ASN A  12       53.37     71.62                                   
REMARK 500 20 PRO A  15        0.64    -69.73                                   
REMARK 500 20 THR A  35      136.06    -39.31                                   
REMARK 500 20 ALA A  67      -60.12    -99.66                                   
REMARK 500 20 ASN A  69       77.32    -66.40                                   
REMARK 500 20 CYS A  74       90.83    -31.00                                   
REMARK 500 20 ASN A  75       48.98     38.98                                   
REMARK 500 20 ARG A  76      108.62   -170.38                                   
REMARK 500 20 PRO A  77       97.89    -69.71                                   
REMARK 500 20 ASP A  87      161.06    -44.23                                   
REMARK 500 20 PRO A  93     -179.72    -69.78                                   
REMARK 500 20 SER A  95       42.23     38.49                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSS001003928.2   RELATED DB: TARGETDB                    
DBREF  1X5T A    8    90  UNP    Q15427   SF3B4_HUMAN    102    184             
SEQADV 1X5T GLY A    1  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A    2  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A    3  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T GLY A    4  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A    5  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A    6  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T GLY A    7  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A   91  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T GLY A   92  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T PRO A   93  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A   94  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T SER A   95  UNP  Q15427              CLONING ARTIFACT               
SEQADV 1X5T GLY A   96  UNP  Q15427              CLONING ARTIFACT               
SEQRES   1 A   96  GLY SER SER GLY SER SER GLY ILE PHE ILE GLY ASN LEU          
SEQRES   2 A   96  ASP PRO GLU ILE ASP GLU LYS LEU LEU TYR ASP THR PHE          
SEQRES   3 A   96  SER ALA PHE GLY VAL ILE LEU GLN THR PRO LYS ILE MET          
SEQRES   4 A   96  ARG ASP PRO ASP THR GLY ASN SER LYS GLY TYR ALA PHE          
SEQRES   5 A   96  ILE ASN PHE ALA SER PHE ASP ALA SER ASP ALA ALA ILE          
SEQRES   6 A   96  GLU ALA MET ASN GLY GLN TYR LEU CYS ASN ARG PRO ILE          
SEQRES   7 A   96  THR VAL SER TYR ALA PHE LYS LYS ASP SER LYS GLY SER          
SEQRES   8 A   96  GLY PRO SER SER GLY                                          
HELIX    1   1 GLU A   19  SER A   27  1                                   9    
HELIX    2   2 SER A   57  ALA A   67  1                                  11    
SHEET    1   A 4 LYS A  37  ILE A  38  0                                        
SHEET    2   A 4 TYR A  50  ASN A  54 -1  O  PHE A  52   N  LYS A  37           
SHEET    3   A 4 GLY A   7  GLY A  11 -1  N  ILE A   8   O  ILE A  53           
SHEET    4   A 4 THR A  79  TYR A  82 -1  O  SER A  81   N  PHE A   9           
SHEET    1   B 2 TYR A  72  LEU A  73  0                                        
SHEET    2   B 2 ARG A  76  PRO A  77 -1  O  ARG A  76   N  LEU A  73           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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