GenomeNet

Database: PDB
Entry: 1Z23
LinkDB: 1Z23
Original site: 1Z23 
HEADER    CELL ADHESION                           07-MAR-05   1Z23              
TITLE     THE SERINE-RICH DOMAIN FROM CRK-ASSOCIATED SUBSTRATE                  
TITLE    2 (P130CAS)                                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CRK-ASSOCIATED SUBSTRATE;                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: SERINE-RICH DOMAIN, SRR-B, RESIDUES 546-708;               
COMPND   5 SYNONYM: P130CAS, BREAST CANCER ANTI-ESTROGEN RESISTANCE 1           
COMPND   6 PROTEIN;                                                             
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE   3 ORGANISM_COMMON: NORWAY RAT;                                         
SOURCE   4 ORGANISM_TAXID: 10116;                                               
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)                                  
KEYWDS    FOUR-HELIX BUNDLE, CELL ADHESION                                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.BRIKNAROVA,F.NASERTORABI,M.L.HAVERT,E.EGGLESTON,D.W.HOYT,           
AUTHOR   2 C.LI,A.J.OLSON,K.VUORI,K.R.ELY                                       
REVDAT   3   24-FEB-09 1Z23    1       VERSN                                    
REVDAT   2   02-AUG-05 1Z23    1       JRNL                                     
REVDAT   1   05-APR-05 1Z23    0                                                
JRNL        AUTH   K.BRIKNAROVA,F.NASERTORABI,M.L.HAVERT,E.EGGLESTON,           
JRNL        AUTH 2 D.W.HOYT,C.LI,A.J.OLSON,K.VUORI,K.R.ELY                      
JRNL        TITL   THE SERINE-RICH DOMAIN FROM CRK-ASSOCIATED                   
JRNL        TITL 2 SUBSTRATE (P130CAS) IS A FOUR-HELIX BUNDLE.                  
JRNL        REF    J.BIOL.CHEM.                  V. 280 21908 2005              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   15795225                                                     
JRNL        DOI    10.1074/JBC.M501258200                                       
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   M.GARCIA-GUZMAN,F.DOLFI,M.RUSSELLO,K.VUORI                   
REMARK   1  TITL   CELL ADHESION REGULATES THE INTERACTIONS BETWEEN             
REMARK   1  TITL 2 THE DOCKING PROTEIN P130(CAS) AND THE 14-3-3                 
REMARK   1  TITL 3 PROTEINS                                                     
REMARK   1  REF    J.BIOL.CHEM.                  V. 274  5762 1999              
REMARK   1  REFN                   ISSN 0021-9258                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : XPLOR-NIH 2.9.4A                                     
REMARK   3   AUTHORS     : C.D.SCHWIETERS,J.J.KUSZEWSKI,N.TJANDRA,G.M.CLORE     
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE CALCULATION EMPLOYED A TOTAL OF       
REMARK   3  1562 UNAMBIGUOUS DISTANCE RESTRAINTS (166 LONG RANGE, 245           
REMARK   3  MEDIUM RANGE, 346 SEQUENTIAL AND 805 INTRARESIDUAL), 6              
REMARK   3  AMBIGUOUS LONG-RANGE DISTANCE RESTRAINTS, 176 HYDROGEN BOND         
REMARK   3  RESTRAINTS, 107 PHI AND 107 PSI DIHEDRAL ANGLE RESTRAINTS, AND      
REMARK   3  79 RESIDUAL DIPOLAR COUPLING RESTRAINTS.                            
REMARK   4                                                                      
REMARK   4 1Z23 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 14-MAR-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB032200.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.9                                
REMARK 210  IONIC STRENGTH                 : 0.1                                
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.8MM PROTEIN U-15N, 20MM          
REMARK 210                                   TRIS U-2H, PH 7.9, 100MM NACL,     
REMARK 210                                   5MM BETA-MERCAPTOETHANOL, 1MM      
REMARK 210                                   4-(2-AMINOETHYL)BENZENE            
REMARK 210                                   FLUORIDE (AEBSF), 90% H2O, 10%     
REMARK 210                                   D2O;; 1.8MM PROTEIN U-13C, U-      
REMARK 210                                   15N, 20MM TRIS U-2H, PH 7.9,       
REMARK 210                                   100MM NACL, 5MM BETA-              
REMARK 210                                   MERCAPTOETHANOL, 1MM 4-(2-         
REMARK 210                                   AMINOETHYL)BENZENE FLUORIDE        
REMARK 210                                   (AEBSF), 90% H2O, 10% D2O;         
REMARK 210                                   1.8MM PROTEIN U-13C, U-15N, ~      
REMARK 210                                   60% 2H, 20MM TRIS U-2H, PH         
REMARK 210                                   7.9, 100MM NACL, 5MM BETA-         
REMARK 210                                   MERCAPTOETHANOL, 1MM 4-(2-         
REMARK 210                                   AMINOETHYL)BENZENE FLUORIDE        
REMARK 210                                   (AEBSF), 90% H2O, 10% D2O;         
REMARK 210                                   1.8MM PROTEIN U-15N, ~60% 2H,      
REMARK 210                                   20MM TRIS U-2H, PH 7.9, 100MM      
REMARK 210                                   NACL, 5MM BETA-                    
REMARK 210                                   MERCAPTOETHANOL, 1MM 4-(2-         
REMARK 210                                   AMINOETHYL)BENZENE FLUORIDE        
REMARK 210                                   (AEBSF), 90% H2O, 10% D2O          
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY, 4D_13C-       
REMARK 210                                   SEPARATED_NOESY                    
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ, 750 MHZ          
REMARK 210  SPECTROMETER MODEL             : UNITYPLUS, INOVA                   
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : FELIX 2000, XPLOR-NIH 2.9.4A       
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 99                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HIS A   610     OH   TYR A   650              2.10            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A 547       94.92     48.96                                   
REMARK 500  1 TRP A 588      -62.98    -94.48                                   
REMARK 500  1 SER A 590       58.62   -149.42                                   
REMARK 500  1 THR A 591     -169.19     46.27                                   
REMARK 500  1 GLU A 596      -46.85    176.43                                   
REMARK 500  1 HIS A 629       99.12    176.10                                   
REMARK 500  1 ARG A 633      -28.76   -164.49                                   
REMARK 500  1 PHE A 703      -80.66   -121.07                                   
REMARK 500  1 ARG A 704      -81.78   -173.11                                   
REMARK 500  1 ARG A 705      104.32     57.47                                   
REMARK 500  2 THR A 591       -2.62     61.22                                   
REMARK 500  2 PRO A 594      -77.01    -80.34                                   
REMARK 500  2 THR A 628      178.89     50.98                                   
REMARK 500  2 SER A 631     -163.11     53.46                                   
REMARK 500  2 SER A 662       21.94     45.78                                   
REMARK 500  2 ARG A 664      138.36     60.65                                   
REMARK 500  2 PHE A 703      -74.57   -118.56                                   
REMARK 500  2 ARG A 704       66.69    -63.43                                   
REMARK 500  3 GLU A 593       69.96   -171.21                                   
REMARK 500  3 HIS A 629      -12.80    170.74                                   
REMARK 500  3 THR A 630      -92.94     46.88                                   
REMARK 500  3 SER A 631     -125.73     47.13                                   
REMARK 500  3 ARG A 633       -7.71     65.77                                   
REMARK 500  3 ASP A 661     -104.72   -169.56                                   
REMARK 500  3 THR A 670      149.30     66.70                                   
REMARK 500  3 PHE A 703      -99.07   -122.11                                   
REMARK 500  3 ARG A 704       62.48   -171.87                                   
REMARK 500  4 PRO A 551      165.15    -40.31                                   
REMARK 500  4 SER A 581       17.45     45.55                                   
REMARK 500  4 ALA A 582      102.49     54.37                                   
REMARK 500  4 GLU A 593       71.15     52.14                                   
REMARK 500  4 GLU A 596      -60.58   -159.08                                   
REMARK 500  4 SER A 625     -146.80     51.55                                   
REMARK 500  4 ALA A 627      -13.75     70.07                                   
REMARK 500  4 PHE A 703      -97.54   -104.89                                   
REMARK 500  4 ARG A 704     -101.77   -139.95                                   
REMARK 500  5 ALA A 582      143.44     61.70                                   
REMARK 500  5 ARG A 589      -22.29   -172.11                                   
REMARK 500  5 PHE A 703      -63.54   -128.88                                   
REMARK 500  5 THR A 706      100.31   -166.66                                   
REMARK 500  6 ARG A 549       74.30     46.72                                   
REMARK 500  6 ARG A 589      104.47     55.33                                   
REMARK 500  6 PRO A 594     -178.27    -69.56                                   
REMARK 500  6 GLN A 595     -113.52    -60.00                                   
REMARK 500  6 PRO A 598       95.00    -41.28                                   
REMARK 500  6 ALA A 627     -139.06    -77.43                                   
REMARK 500  6 THR A 628      100.68     53.90                                   
REMARK 500  6 HIS A 629       -8.92   -167.92                                   
REMARK 500  6 SER A 631       22.24    -72.23                                   
REMARK 500  6 ASP A 632       95.91     51.89                                   
REMARK 500  6 SER A 662      126.64     74.76                                   
REMARK 500  6 PHE A 703      -72.80   -123.57                                   
REMARK 500  6 ARG A 704      106.82     75.53                                   
REMARK 500  6 LYS A 707      -86.17     53.52                                   
REMARK 500  7 SER A 547       29.21     47.13                                   
REMARK 500  7 PRO A 551     -164.99    -76.45                                   
REMARK 500  7 LEU A 552     -178.57    -69.98                                   
REMARK 500  7 SER A 583      104.48     56.08                                   
REMARK 500  7 ARG A 589      109.01   -163.15                                   
REMARK 500  7 GLN A 595     -147.98   -123.66                                   
REMARK 500  7 SER A 626       16.37     56.92                                   
REMARK 500  7 ALA A 627      -81.91    -70.72                                   
REMARK 500  7 HIS A 629      -85.55    -90.64                                   
REMARK 500  7 PHE A 669      109.67   -170.59                                   
REMARK 500  7 PHE A 703      -70.80   -118.32                                   
REMARK 500  7 ARG A 704      101.93     72.95                                   
REMARK 500  7 LYS A 707     -156.24     46.22                                   
REMARK 500  8 SER A 592       94.96     48.63                                   
REMARK 500  8 GLN A 595     -115.11     45.84                                   
REMARK 500  8 THR A 630      -80.43   -140.15                                   
REMARK 500  8 SER A 631       45.95   -144.60                                   
REMARK 500  8 ARG A 633      152.89    -47.61                                   
REMARK 500  8 ASP A 661     -129.66   -170.40                                   
REMARK 500  8 PHE A 703      -79.91   -120.84                                   
REMARK 500  8 ARG A 704     -100.32   -172.33                                   
REMARK 500  8 ARG A 705      -62.75   -161.42                                   
REMARK 500  9 ARG A 549      -72.90     61.10                                   
REMARK 500  9 GLU A 550       91.01     52.17                                   
REMARK 500  9 ALA A 582      -91.44     51.83                                   
REMARK 500  9 SER A 592     -148.93     59.76                                   
REMARK 500  9 GLU A 596      155.74     56.17                                   
REMARK 500  9 ASP A 632       41.07     73.58                                   
REMARK 500  9 SER A 662     -112.95   -167.47                                   
REMARK 500  9 PHE A 703      -84.13   -118.06                                   
REMARK 500  9 ARG A 704     -106.94   -148.64                                   
REMARK 500  9 ARG A 705       66.85   -167.34                                   
REMARK 500  9 THR A 706     -170.70     46.35                                   
REMARK 500 10 ARG A 549      -62.70   -157.77                                   
REMARK 500 10 GLU A 553      112.71     87.83                                   
REMARK 500 10 SER A 583      167.03     55.94                                   
REMARK 500 10 TRP A 588     -164.84     49.00                                   
REMARK 500 10 THR A 591     -117.27    -99.08                                   
REMARK 500 10 SER A 592     -177.45     54.35                                   
REMARK 500 10 HIS A 629     -157.56    -92.82                                   
REMARK 500 10 THR A 630      -39.38     71.25                                   
REMARK 500 10 PHE A 703      -68.72   -121.66                                   
REMARK 500 10 ARG A 704       99.18     72.40                                   
REMARK 500 11 GLU A 550      -62.19   -152.83                                   
REMARK 500 11 PRO A 551      152.31    -30.78                                   
REMARK 500 11 GLU A 553       90.68     91.97                                   
REMARK 500 11 TRP A 588      -72.79    -89.23                                   
REMARK 500 11 ARG A 589     -160.90     42.54                                   
REMARK 500 11 PRO A 598      105.30    -45.07                                   
REMARK 500 11 ARG A 633      -12.47   -154.47                                   
REMARK 500 11 ASP A 661       24.73    -73.91                                   
REMARK 500 11 PHE A 703     -112.12   -121.51                                   
REMARK 500 11 ARG A 704      -96.04   -173.12                                   
REMARK 500 12 ARG A 549     -158.77     46.03                                   
REMARK 500 12 ALA A 582     -157.11    -80.82                                   
REMARK 500 12 SER A 583      167.35     61.98                                   
REMARK 500 12 SER A 590       71.16    -63.25                                   
REMARK 500 12 GLU A 593       72.38   -161.31                                   
REMARK 500 12 GLN A 595     -145.41    -98.66                                   
REMARK 500 12 THR A 630       -1.95     62.68                                   
REMARK 500 12 SER A 631       56.01   -117.87                                   
REMARK 500 12 PRO A 667      151.05    -49.34                                   
REMARK 500 12 ARG A 705     -176.70     49.60                                   
REMARK 500 13 PRO A 551      -94.88    -85.46                                   
REMARK 500 13 SER A 583       90.50     44.22                                   
REMARK 500 13 SER A 592      151.35    164.47                                   
REMARK 500 13 GLU A 593       75.08     48.09                                   
REMARK 500 13 ALA A 627      -96.68    -59.88                                   
REMARK 500 13 THR A 630     -166.81     47.32                                   
REMARK 500 13 SER A 631       87.48     46.38                                   
REMARK 500 13 PHE A 703      -74.27   -122.56                                   
REMARK 500 13 ARG A 704     -118.26    -89.87                                   
REMARK 500 13 ARG A 705      -25.76   -171.30                                   
REMARK 500 14 SER A 583       96.83     50.47                                   
REMARK 500 14 SER A 590      -74.53    -73.18                                   
REMARK 500 14 PRO A 598        8.05    -53.00                                   
REMARK 500 14 THR A 628       -4.01   -177.07                                   
REMARK 500 14 THR A 630      165.27     57.83                                   
REMARK 500 14 ASP A 661     -117.49   -173.50                                   
REMARK 500 14 SER A 662     -120.48   -153.88                                   
REMARK 500 14 PHE A 703      -88.11   -118.56                                   
REMARK 500 14 ARG A 704      105.98   -173.14                                   
REMARK 500 15 ALA A 582     -175.00     48.98                                   
REMARK 500 15 ARG A 589      -86.52   -146.23                                   
REMARK 500 15 SER A 631       20.24     48.87                                   
REMARK 500 15 ASP A 632      173.21     52.62                                   
REMARK 500 15 PHE A 703      -72.82   -117.12                                   
REMARK 500 16 GLU A 550       80.16     49.38                                   
REMARK 500 16 ALA A 582     -124.59     47.32                                   
REMARK 500 16 SER A 583       93.64     47.10                                   
REMARK 500 16 TRP A 588      176.93     53.11                                   
REMARK 500 16 SER A 592      101.92     53.92                                   
REMARK 500 16 THR A 628       33.62   -168.15                                   
REMARK 500 16 SER A 631     -129.76   -172.29                                   
REMARK 500 16 THR A 706      -27.47   -174.90                                   
REMARK 500 17 SER A 547      170.90     54.27                                   
REMARK 500 17 ARG A 549     -138.97    -94.89                                   
REMARK 500 17 PRO A 551      -92.30    -71.65                                   
REMARK 500 17 SER A 581      -22.32     72.60                                   
REMARK 500 17 ALA A 582       13.21     48.72                                   
REMARK 500 17 GLN A 595      101.38   -170.04                                   
REMARK 500 17 GLU A 596       79.81     48.16                                   
REMARK 500 17 THR A 628      -88.68     49.30                                   
REMARK 500 17 ASP A 661     -122.81    -84.88                                   
REMARK 500 17 SER A 662      158.48     58.03                                   
REMARK 500 17 PHE A 703      -71.83   -125.15                                   
REMARK 500 17 ARG A 704      108.70     77.49                                   
REMARK 500 18 SER A 547      -57.47   -144.69                                   
REMARK 500 18 GLU A 550       79.00     55.19                                   
REMARK 500 18 PRO A 551     -152.82    -61.19                                   
REMARK 500 18 PRO A 594      155.15    -42.48                                   
REMARK 500 18 SER A 625       69.38     31.46                                   
REMARK 500 18 SER A 626      -66.14     72.69                                   
REMARK 500 18 ALA A 627       -8.97     67.14                                   
REMARK 500 18 HIS A 629     -127.82   -171.83                                   
REMARK 500 18 SER A 662       84.49     46.33                                   
REMARK 500 18 PHE A 669      119.63     59.15                                   
REMARK 500 18 THR A 670     -158.88   -141.04                                   
REMARK 500 18 PHE A 703     -101.71   -126.38                                   
REMARK 500 18 ARG A 704      106.27   -171.43                                   
REMARK 500 19 GLU A 553      103.91     87.19                                   
REMARK 500 19 SER A 592     -177.84     50.26                                   
REMARK 500 19 GLU A 596      -64.90   -148.19                                   
REMARK 500 19 SER A 662       92.56     47.63                                   
REMARK 500 19 ARG A 664       76.89     45.35                                   
REMARK 500 19 PHE A 669       85.01     50.12                                   
REMARK 500 19 THR A 670     -128.11   -153.00                                   
REMARK 500 19 PHE A 703      -66.68   -125.67                                   
REMARK 500 19 ARG A 704      110.36     79.34                                   
REMARK 500 20 PRO A 551      166.59    -44.16                                   
REMARK 500 20 ALA A 582      178.89     72.62                                   
REMARK 500 20 ARG A 589       93.37     46.79                                   
REMARK 500 20 SER A 592      113.91     69.90                                   
REMARK 500 20 PRO A 598      152.49    -42.37                                   
REMARK 500 20 SER A 625       43.41     32.78                                   
REMARK 500 20 THR A 630     -158.28   -136.44                                   
REMARK 500 20 ARG A 633      -19.08   -141.19                                   
REMARK 500 20 THR A 670     -114.90   -167.53                                   
REMARK 500 20 PHE A 703      -75.19   -116.53                                   
REMARK 500 20 ARG A 704       64.51    -67.51                                   
REMARK 500 20 LYS A 707       92.42   -162.82                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1Z23 A  546   708  UNP    Q63767   BCA1_RAT       546    708             
SEQADV 1Z23 GLY A  546  UNP  Q63767    VAL   546 ENGINEERED                     
SEQADV 1Z23 SER A  547  UNP  Q63767    PRO   547 ENGINEERED                     
SEQRES   1 A  163  GLY SER GLY ARG GLU PRO LEU GLU LEU GLU VAL ALA VAL          
SEQRES   2 A  163  GLU THR LEU ALA ARG LEU GLN GLN GLY VAL SER THR THR          
SEQRES   3 A  163  VAL ALA HIS LEU LEU ASP LEU VAL GLY SER ALA SER GLY          
SEQRES   4 A  163  PRO GLY GLY TRP ARG SER THR SER GLU PRO GLN GLU PRO          
SEQRES   5 A  163  PRO VAL GLN ASP LEU LYS ALA ALA VAL ALA ALA VAL HIS          
SEQRES   6 A  163  GLY ALA VAL HIS GLU LEU LEU GLU PHE ALA ARG SER ALA          
SEQRES   7 A  163  VAL SER SER ALA THR HIS THR SER ASP ARG THR LEU HIS          
SEQRES   8 A  163  ALA LYS LEU SER ARG GLN LEU GLN LYS MET GLU ASP VAL          
SEQRES   9 A  163  TYR GLN THR LEU VAL VAL HIS GLY GLN VAL LEU ASP SER          
SEQRES  10 A  163  GLY ARG GLY GLY PRO GLY PHE THR LEU ASP ASP LEU ASP          
SEQRES  11 A  163  ARG LEU VAL ALA CYS SER ARG ALA VAL PRO GLU ASP ALA          
SEQRES  12 A  163  LYS GLN LEU ALA SER PHE LEU HIS GLY ASN ALA SER LEU          
SEQRES  13 A  163  LEU PHE ARG ARG THR LYS ALA                                  
HELIX    1   1 GLU A  553  ALA A  582  1                                  30    
HELIX    2   2 PRO A  598  SER A  625  1                                  28    
HELIX    3   3 ARG A  633  SER A  662  1                                  30    
HELIX    4   4 THR A  670  PHE A  703  1                                  34    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system