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Database: PDB
Entry: 2CL0
LinkDB: 2CL0
Original site: 2CL0 
HEADER    NUCLEOTIDE BINDING PROTEIN              25-APR-06   2CL0              
TITLE     CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS             
TITLE    2 P21 IN COMPLEX WITH GPPNHP                                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GTPASE HRAS;                                               
COMPND   3 CHAIN: X;                                                            
COMPND   4 FRAGMENT: RESIDUES 1-166;                                            
COMPND   5 SYNONYM: TRANSFORMING PROTEIN P21, P21RAS, H-RAS-1, C-H-RAS;         
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES;                                                       
COMPND   8 OTHER_DETAILS: IANBD FLUOROPHORE WAS ATTACHED TO CYS-32              
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: CK600K;                                    
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PTAC RAS                                  
KEYWDS    NUCLEOTIDE BINDING PROTEIN, METHYLATION, LIPOPROTEIN, GTP-BINDING,    
KEYWDS   2 FLUORESCENCE, GPPNHP, MEMBRANE, PALMITATE, PRENYLATION,              
KEYWDS   3 PROTO-ONCOGENE, GOLGI APPARATUS, DISEASE MUTATION,                   
KEYWDS   4 NUCLEOTIDE-BINDING, GUANINE NUCLEOTIDE BINDING PROTEIN               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    B.U.KLINK,R.S.GOODY,A.J.SCHEIDIG                                      
REVDAT   5   13-JUL-11 2CL0    1       VERSN                                    
REVDAT   4   24-FEB-09 2CL0    1       VERSN                                    
REVDAT   3   23-AUG-06 2CL0    1       JRNL                                     
REVDAT   2   07-JUN-06 2CL0    1       JRNL                                     
REVDAT   1   31-MAY-06 2CL0    0                                                
JRNL        AUTH   B.U.KLINK,R.S.GOODY,A.J.SCHEIDIG                             
JRNL        TITL   A NEWLY DESIGNED MICROSPECTROFLUOROMETER FOR KINETIC         
JRNL        TITL 2 STUDIES ON PROTEIN CRYSTALS IN COMBINATION WITH X-RAY        
JRNL        TITL 3 DIFFRACTION                                                  
JRNL        REF    BIOPHYS.J.                    V.  91   981 2006              
JRNL        REFN                   ISSN 0006-3495                               
JRNL        PMID   16698776                                                     
JRNL        DOI    10.1529/BIOPHYSJ.105.078931                                  
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.8  ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.2.0005                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.80                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 67.42                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9                           
REMARK   3   NUMBER OF REFLECTIONS             : 19368                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.148                           
REMARK   3   R VALUE            (WORKING SET) : 0.146                           
REMARK   3   FREE R VALUE                     : 0.185                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.0                             
REMARK   3   FREE R VALUE TEST SET COUNT      : 1030                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.80                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.85                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 1408                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.1890                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 92                           
REMARK   3   BIN FREE R VALUE                    : 0.2370                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1534                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 66                                      
REMARK   3   SOLVENT ATOMS            : 168                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   B VALUE TYPE : LIKELY RESIDUAL                                     
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 28.74                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -0.88000                                             
REMARK   3    B22 (A**2) : -0.88000                                             
REMARK   3    B33 (A**2) : 1.32000                                              
REMARK   3    B12 (A**2) : -0.44000                                             
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.101         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.102         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.067         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 4.737         
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.971                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.961                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  1631 ; 0.016 ; 0.021       
REMARK   3   BOND LENGTHS OTHERS               (A):  1423 ; 0.003 ; 0.020       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  2230 ; 1.815 ; 2.001       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  3323 ; 0.890 ; 3.000       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   204 ; 5.911 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    86 ;34.670 ;23.953       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   292 ;16.242 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    17 ;20.662 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   230 ; 0.108 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  1910 ; 0.008 ; 0.020       
REMARK   3   GENERAL PLANES OTHERS             (A):   345 ; 0.002 ; 0.020       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):   384 ; 0.210 ; 0.200       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  1558 ; 0.204 ; 0.200       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):   807 ; 0.177 ; 0.200       
REMARK   3   NON-BONDED TORSION OTHERS         (A):   919 ; 0.086 ; 0.200       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   142 ; 0.174 ; 0.200       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    33 ; 0.370 ; 0.200       
REMARK   3   SYMMETRY VDW OTHERS               (A):    81 ; 0.421 ; 0.200       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):    28 ; 0.289 ; 0.200       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  1271 ; 1.371 ; 1.500       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  1569 ; 1.582 ; 2.000       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):   790 ; 2.538 ; 3.000       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):   661 ; 3.754 ; 4.500       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   X     1        X   166                          
REMARK   3    ORIGIN FOR THE GROUP (A):  41.6070  55.6450  37.2000              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0043 T22:  -0.0310                                     
REMARK   3      T33:  -0.0826 T12:   0.0034                                     
REMARK   3      T13:  -0.0065 T23:   0.0213                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7981 L22:   2.9406                                     
REMARK   3      L33:   1.0249 L12:   0.1375                                     
REMARK   3      L13:  -0.1324 L23:   0.4051                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0008 S12:  -0.1198 S13:  -0.0106                       
REMARK   3      S21:   0.3846 S22:   0.0333 S23:   0.0019                       
REMARK   3      S31:   0.1401 S32:   0.0007 S33:  -0.0325                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   W  2001        W  2168                          
REMARK   3    ORIGIN FOR THE GROUP (A):  39.2360  56.9560  35.0260              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  -0.0760 T22:  -0.0068                                     
REMARK   3      T33:  -0.0972 T12:   0.0024                                     
REMARK   3      T13:   0.0108 T23:   0.0138                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.8635 L22:   2.7434                                     
REMARK   3      L33:   1.0451 L12:  -0.0882                                     
REMARK   3      L13:  -0.0421 L23:  -0.0306                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0192 S12:  -0.0850 S13:  -0.0179                       
REMARK   3      S21:   0.1791 S22:   0.0194 S23:   0.1549                       
REMARK   3      S31:   0.0039 S32:  -0.0457 S33:  -0.0002                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   X   200        X   200                          
REMARK   3    ORIGIN FOR THE GROUP (A):  33.2010  51.6320  44.7260              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1681 T22:   0.1040                                     
REMARK   3      T33:   0.0013 T12:  -0.0730                                     
REMARK   3      T13:   0.1352 T23:   0.0498                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7546 L22:   2.8542                                     
REMARK   3      L33:   2.0102 L12:  -0.2849                                     
REMARK   3      L13:  -0.1710 L23:  -0.3859                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1265 S12:  -0.1478 S13:  -0.1289                       
REMARK   3      S21:   0.6917 S22:   0.1541 S23:   0.5623                       
REMARK   3      S31:   0.3066 S32:  -0.3826 S33:  -0.0276                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   X   332        X   332                          
REMARK   3    ORIGIN FOR THE GROUP (A):  32.9900  63.0000  41.4260              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0342 T22:   0.0055                                     
REMARK   3      T33:  -0.0169 T12:   0.0038                                     
REMARK   3      T13:   0.0657 T23:   0.0095                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.3044 L22:   2.7537                                     
REMARK   3      L33:   7.7298 L12:  -2.2710                                     
REMARK   3      L13:   3.8504 L23:  -2.3697                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0393 S12:  -0.2397 S13:   0.1208                       
REMARK   3      S21:   0.4954 S22:   0.0367 S23:   0.2817                       
REMARK   3      S31:  -0.1842 S32:  -0.2546 S33:   0.0026                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : BABINET MODEL WITH MASK                              
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.20                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE          
REMARK   3  RIDING POSITIONS.                                                   
REMARK   4                                                                      
REMARK   4 2CL0 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 25-APR-06.                  
REMARK 100 THE PDBE ID CODE IS EBI-28555.                                       
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 18-DEC-02                          
REMARK 200  TEMPERATURE           (KELVIN) : 100.0                              
REMARK 200  PH                             : 7.60                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID14-4                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.934                              
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC                               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XSCALE                             
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 23933                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.700                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 67.420                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 11.600                             
REMARK 200  R MERGE                    (I) : 0.08000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 16.9000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.70                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.75                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 88.8                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.17                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.88000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.050                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: MOLREP                                                
REMARK 200 STARTING MODEL: PDB ENTRY 5P21                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 56.5                                      
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.9                      
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: RESERVOIR SOLUTION: 64 MM TRIS           
REMARK 280  PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0.1 MM SODIUM          
REMARK 280  AZIDE, 26% PEG 400;  PROTEIN SOLUTION: 64 MM TRIS PH 7.6,           
REMARK 280  20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0.1 MM SODIUM AZIDE            
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: H 3 2                            
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z                                                
REMARK 290       3555   -X+Y,-X,Z                                               
REMARK 290       4555   Y,X,-Z                                                  
REMARK 290       5555   X-Y,-Y,-Z                                               
REMARK 290       6555   -X,-X+Y,-Z                                              
REMARK 290       7555   X+2/3,Y+1/3,Z+1/3                                       
REMARK 290       8555   -Y+2/3,X-Y+1/3,Z+1/3                                    
REMARK 290       9555   -X+Y+2/3,-X+1/3,Z+1/3                                   
REMARK 290      10555   Y+2/3,X+1/3,-Z+1/3                                      
REMARK 290      11555   X-Y+2/3,-Y+1/3,-Z+1/3                                   
REMARK 290      12555   -X+2/3,-X+Y+1/3,-Z+1/3                                  
REMARK 290      13555   X+1/3,Y+2/3,Z+2/3                                       
REMARK 290      14555   -Y+1/3,X-Y+2/3,Z+2/3                                    
REMARK 290      15555   -X+Y+1/3,-X+2/3,Z+2/3                                   
REMARK 290      16555   Y+1/3,X+2/3,-Z+2/3                                      
REMARK 290      17555   X-Y+1/3,-Y+2/3,-Z+2/3                                   
REMARK 290      18555   -X+1/3,-X+Y+2/3,-Z+2/3                                  
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   5  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   6 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   7  1.000000  0.000000  0.000000       44.27000            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       25.55930            
REMARK 290   SMTRY3   7  0.000000  0.000000  1.000000       48.03333            
REMARK 290   SMTRY1   8 -0.500000 -0.866025  0.000000       44.27000            
REMARK 290   SMTRY2   8  0.866025 -0.500000  0.000000       25.55930            
REMARK 290   SMTRY3   8  0.000000  0.000000  1.000000       48.03333            
REMARK 290   SMTRY1   9 -0.500000  0.866025  0.000000       44.27000            
REMARK 290   SMTRY2   9 -0.866025 -0.500000  0.000000       25.55930            
REMARK 290   SMTRY3   9  0.000000  0.000000  1.000000       48.03333            
REMARK 290   SMTRY1  10 -0.500000  0.866025  0.000000       44.27000            
REMARK 290   SMTRY2  10  0.866025  0.500000  0.000000       25.55930            
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000       48.03333            
REMARK 290   SMTRY1  11  1.000000  0.000000  0.000000       44.27000            
REMARK 290   SMTRY2  11  0.000000 -1.000000  0.000000       25.55930            
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000       48.03333            
REMARK 290   SMTRY1  12 -0.500000 -0.866025  0.000000       44.27000            
REMARK 290   SMTRY2  12 -0.866025  0.500000  0.000000       25.55930            
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000       48.03333            
REMARK 290   SMTRY1  13  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  13  0.000000  1.000000  0.000000       51.11859            
REMARK 290   SMTRY3  13  0.000000  0.000000  1.000000       96.06667            
REMARK 290   SMTRY1  14 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  14  0.866025 -0.500000  0.000000       51.11859            
REMARK 290   SMTRY3  14  0.000000  0.000000  1.000000       96.06667            
REMARK 290   SMTRY1  15 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  15 -0.866025 -0.500000  0.000000       51.11859            
REMARK 290   SMTRY3  15  0.000000  0.000000  1.000000       96.06667            
REMARK 290   SMTRY1  16 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  16  0.866025  0.500000  0.000000       51.11859            
REMARK 290   SMTRY3  16  0.000000  0.000000 -1.000000       96.06667            
REMARK 290   SMTRY1  17  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  17  0.000000 -1.000000  0.000000       51.11859            
REMARK 290   SMTRY3  17  0.000000  0.000000 -1.000000       96.06667            
REMARK 290   SMTRY1  18 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  18 -0.866025  0.500000  0.000000       51.11859            
REMARK 290   SMTRY3  18  0.000000  0.000000 -1.000000       96.06667            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7980 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 21350 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -151.2 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: X                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -0.500000  0.866025  0.000000        0.00000            
REMARK 350   BIOMT2   2 -0.866025 -0.500000  0.000000      153.35578            
REMARK 350   BIOMT3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   3 -0.500000 -0.866025  0.000000      132.81000            
REMARK 350   BIOMT2   3  0.866025 -0.500000  0.000000       76.67789            
REMARK 350   BIOMT3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375      HOH X2026  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH X2153  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH X2155  LIES ON A SPECIAL POSITION.                          
REMARK 400                                                                      
REMARK 400 COMPOUND                                                             
REMARK 400 ENGINEERED RESIDUE IN CHAIN X, TYR 32 TO CYS                         
REMARK 400 ENGINEERED RESIDUE IN CHAIN X, CYS 118 TO SER                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OE1  GLN X    61     O    HOH X  2071              1.90            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    CYS X  51   CB    CYS X  51   SG     -0.174                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS X 117       35.28     72.03                                   
REMARK 500    ARG X 149       -4.67     73.68                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620  (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;              
REMARK 620   SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                           
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG X 167  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH X2119   O                                                      
REMARK 620 2 HOH X2119   O    91.0                                              
REMARK 620 3 HOH X2110   O    92.3  91.8                                        
REMARK 620 4 HOH X2110   O   170.1  79.4  90.4                                  
REMARK 620 5 HOH X2119   O    91.3  85.0 175.3  85.6                            
REMARK 620 6 HOH X2110   O    99.2 165.7  97.8  89.9  84.8                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG X 168  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 SER X  17   OG                                                     
REMARK 620 2 GNP X 200   O2B  92.7                                              
REMARK 620 3 HOH X2067   O    83.3  92.7                                        
REMARK 620 4 GNP X 200   O1G 172.9  90.1  90.1                                  
REMARK 620 5 HOH X2034   O    85.7 172.9  94.0  92.3                            
REMARK 620 6 HOH X2161   O    92.1  86.0 175.1  94.6  87.2                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG X 169  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH X2123   O                                                      
REMARK 620 2 HOH X2122   O    92.6                                              
REMARK 620 3 ASP X 132   OD1  99.5  88.7                                        
REMARK 620 4 HOH X2112   O    91.3  90.8 169.2                                  
REMARK 620 5 HOH X2113   O    89.2 178.2  91.5  88.7                            
REMARK 620 6 HOH X2121   O   175.7  91.2  82.6  86.7  87.0                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG X 170  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH X2035   O                                                      
REMARK 620 2 GNP X 200   O3G  88.4                                              
REMARK 620 3 HOH X2030   O    98.0  87.7                                        
REMARK 620 4 HOH X2072   O   179.3  91.6  82.7                                  
REMARK 620 5 HOH X2074   O    86.4 174.0  90.0  93.7                            
REMARK 620 6 GLU X  63   OE1  92.3  90.1 169.4  87.0  93.1                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG X 171  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH X2155   O                                                      
REMARK 620 2 HOH X2041   O   160.7                                              
REMARK 620 3 HOH X2132   O    85.5  97.2                                        
REMARK 620 4 HOH X2132   O    78.8  89.6 148.9                                  
REMARK 620 5 HOH X2156   O    81.7  80.0  79.7  71.7                            
REMARK 620 6 HOH X2156   O   117.2  79.8 111.5  99.6 157.9                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  MG X 167                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  MG X 168                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  MG X 169                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  MG X 170                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE  MG X 171                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE TRS X 199                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GNP X 200                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE XY2 X 332                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 121P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1AA9   RELATED DB: PDB                                   
REMARK 900  HUMAN C-HA-RAS(1-171)(DOT)GDP, NMR,                                 
REMARK 900  MINIMIZED AVERAGE STRUCTURE                                         
REMARK 900 RELATED ID: 1AGP   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1BKD   RELATED DB: PDB                                   
REMARK 900  COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1                             
REMARK 900 RELATED ID: 1CLU   RELATED DB: PDB                                   
REMARK 900  H-RAS COMPLEXED WITH DIAMINOBENZOPHENONE-BETA                       
REMARK 900  ,GAMMA-IMIDO- GTP                                                   
REMARK 900 RELATED ID: 1CRP   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1CRQ   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1CRR   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1CTQ   RELATED DB: PDB                                   
REMARK 900  STRUCTURE OF P21RAS IN COMPLEX WITH GPPNHP                          
REMARK 900  AT 100 K                                                            
REMARK 900 RELATED ID: 1GNP   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1GNQ   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1GNR   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1HE8   RELATED DB: PDB                                   
REMARK 900  RAS G12V - PI 3-KINASE GAMMA COMPLEX                                
REMARK 900 RELATED ID: 1IAQ   RELATED DB: PDB                                   
REMARK 900  C-H-RAS P21 PROTEIN MUTANT WITH THR 35                              
REMARK 900  REPLACED BY SER(T35S) COMPLEXED WITH                                
REMARK 900  GUANOSINE-5'-[B,G-IMIDO] TRIPHOSPHATE                               
REMARK 900 RELATED ID: 1IOZ   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF THE C-HA-RAS PROTEIN                           
REMARK 900   PREPARED BY THECELL-FREE SYNTHESIS                                 
REMARK 900 RELATED ID: 1JAH   RELATED DB: PDB                                   
REMARK 900  H-RAS P21 PROTEIN MUTANT G12P, COMPLEXED                            
REMARK 900  WITHGUANOSINE-5'-[BETA,GAMMA-METHYLENE]                             
REMARK 900  TRIPHOSPHATE ANDMAGNESIUM                                           
REMARK 900 RELATED ID: 1JAI   RELATED DB: PDB                                   
REMARK 900  H-RAS P21 PROTEIN MUTANT G12P, COMPLEXED                            
REMARK 900  WITHGUANOSINE-5'-[BETA,GAMMA-METHYLENE]                             
REMARK 900  TRIPHOSPHATEAND MANGANESE                                           
REMARK 900 RELATED ID: 1K8R   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF RAS-BRY2RBD COMPLEX                            
REMARK 900 RELATED ID: 1LF0   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF RASA59G IN THE GTP-                            
REMARK 900  BOUND FORM                                                          
REMARK 900 RELATED ID: 1LF5   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF RASA59G IN THE GDP-                            
REMARK 900  BOUND FORM                                                          
REMARK 900 RELATED ID: 1LFD   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF THE ACTIVE RAS PROTEIN                         
REMARK 900   COMPLEXED WITHTHE RAS-INTERACTING DOMAIN OF                        
REMARK 900   RALGDS                                                             
REMARK 900 RELATED ID: 1NVU   RELATED DB: PDB                                   
REMARK 900  STRUCTURAL EVIDENCE FOR FEEDBACK ACTIVATION BY                      
REMARK 900   RASGTP OFTHE RAS-SPECIFIC NUCLEOTIDE                               
REMARK 900  EXCHANGE FACTOR SOS                                                 
REMARK 900 RELATED ID: 1NVV   RELATED DB: PDB                                   
REMARK 900  STRUCTURAL EVIDENCE FOR FEEDBACK ACTIVATION BY                      
REMARK 900   RASGTP OFTHE RAS-SPECIFIC NUCLEOTIDE                               
REMARK 900  EXCHANGE FACTOR SOS                                                 
REMARK 900 RELATED ID: 1NVW   RELATED DB: PDB                                   
REMARK 900  STRUCTURAL EVIDENCE FOR FEEDBACK ACTIVATION BY                      
REMARK 900   RASGTP OFTHE RAS-SPECIFIC NUCLEOTIDE                               
REMARK 900  EXCHANGE FACTOR SOS                                                 
REMARK 900 RELATED ID: 1NVX   RELATED DB: PDB                                   
REMARK 900  STRUCTURAL EVIDENCE FOR FEEDBACK ACTIVATION BY                      
REMARK 900   RASGTP OFTHE RAS-SPECIFIC NUCLEOTIDE                               
REMARK 900  EXCHANGE FACTOR SOS                                                 
REMARK 900 RELATED ID: 1P2S   RELATED DB: PDB                                   
REMARK 900  H-RAS 166 IN 50% 2,2,2 TRIFLOUROETHANOL                             
REMARK 900 RELATED ID: 1P2T   RELATED DB: PDB                                   
REMARK 900  H-RAS 166 IN AQUEOUS MOTHER LIQOUR, RT                              
REMARK 900 RELATED ID: 1P2U   RELATED DB: PDB                                   
REMARK 900  H-RAS IN 50% ISOPROPANOL                                            
REMARK 900 RELATED ID: 1P2V   RELATED DB: PDB                                   
REMARK 900  H-RAS 166 IN 60 % 1,6 HEXANEDIOL                                    
REMARK 900 RELATED ID: 1PLJ   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1PLK   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1PLL   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1Q21   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1QRA   RELATED DB: PDB                                   
REMARK 900  STRUCTURE OF P21RAS IN COMPLEX WITH GTP AT                          
REMARK 900   100 K                                                              
REMARK 900 RELATED ID: 1RVD   RELATED DB: PDB                                   
REMARK 900  H-RAS COMPLEXED WITH DIAMINOBENZOPHENONE-BETA                       
REMARK 900  ,GAMMA-IMIDO- GTP                                                   
REMARK 900 RELATED ID: 1WQ1   RELATED DB: PDB                                   
REMARK 900  RAS-RASGAP COMPLEX                                                  
REMARK 900 RELATED ID: 1XCM   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF THE GPPNHP-BOUND H-                            
REMARK 900  RAS G60A MUTANT                                                     
REMARK 900 RELATED ID: 1XD2   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF A TERNARY RAS:SOS:RAS                          
REMARK 900  *GDP COMPLEX                                                        
REMARK 900 RELATED ID: 1XJ0   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF THE GDP-BOUND FORM OF                          
REMARK 900   THE RASG60AMUTANT                                                  
REMARK 900 RELATED ID: 1ZVQ   RELATED DB: PDB                                   
REMARK 900  STRUCTURE OF THE Q61G MUTANT OF RAS IN                              
REMARK 900  THE GDP-BOUND FORM                                                  
REMARK 900 RELATED ID: 1ZW6   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF THE GTP-BOUND FORM OF                          
REMARK 900   RASQ61G                                                            
REMARK 900 RELATED ID: 221P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 2C5L   RELATED DB: PDB                                   
REMARK 900  STRUCTURE OF PLC EPSILON RAS ASSOCIATION                            
REMARK 900  DOMAIN WITH HRAS                                                    
REMARK 900 RELATED ID: 2CE2   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT                         
REMARK 900  FORM OF H-RAS P21 IN COMPLEX WITH GDP                               
REMARK 900 RELATED ID: 2CL6   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT                         
REMARK 900  FORM OF H-RAS P21 IN COMPLEX WITH S-                                
REMARK 900  CAGED GTP                                                           
REMARK 900 RELATED ID: 2CL7   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT                         
REMARK 900  FORM OF H-RAS P21 IN COMPLEX WITH GTP                               
REMARK 900 RELATED ID: 2CLC   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT                         
REMARK 900  FORM OF H-RAS P21 IN COMPLEX WITH GTP (                             
REMARK 900  2)                                                                  
REMARK 900 RELATED ID: 2CLD   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT                         
REMARK 900  FORM OF H-RAS P21 IN COMPLEX WITH GDP (                             
REMARK 900  2)                                                                  
REMARK 900 RELATED ID: 2GDP   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 2Q21   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 421P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4Q21   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 521P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5P21   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 621P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 6Q21   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 721P   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 821P   RELATED DB: PDB                                   
DBREF  2CL0 X    1   166  UNP    P01112   RASH_HUMAN       1    166             
SEQADV 2CL0 CYS X   32  UNP  P01112    TYR    32 ENGINEERED MUTATION            
SEQADV 2CL0 SER X  118  UNP  P01112    CYS   118 ENGINEERED MUTATION            
SEQRES   1 X  166  MET THR GLU TYR LYS LEU VAL VAL VAL GLY ALA GLY GLY          
SEQRES   2 X  166  VAL GLY LYS SER ALA LEU THR ILE GLN LEU ILE GLN ASN          
SEQRES   3 X  166  HIS PHE VAL ASP GLU CYS ASP PRO THR ILE GLU ASP SER          
SEQRES   4 X  166  TYR ARG LYS GLN VAL VAL ILE ASP GLY GLU THR CYS LEU          
SEQRES   5 X  166  LEU ASP ILE LEU ASP THR ALA GLY GLN GLU GLU TYR SER          
SEQRES   6 X  166  ALA MET ARG ASP GLN TYR MET ARG THR GLY GLU GLY PHE          
SEQRES   7 X  166  LEU CYS VAL PHE ALA ILE ASN ASN THR LYS SER PHE GLU          
SEQRES   8 X  166  ASP ILE HIS GLN TYR ARG GLU GLN ILE LYS ARG VAL LYS          
SEQRES   9 X  166  ASP SER ASP ASP VAL PRO MET VAL LEU VAL GLY ASN LYS          
SEQRES  10 X  166  SER ASP LEU ALA ALA ARG THR VAL GLU SER ARG GLN ALA          
SEQRES  11 X  166  GLN ASP LEU ALA ARG SER TYR GLY ILE PRO TYR ILE GLU          
SEQRES  12 X  166  THR SER ALA LYS THR ARG GLN GLY VAL GLU ASP ALA PHE          
SEQRES  13 X  166  TYR THR LEU VAL ARG GLU ILE ARG GLN HIS                      
HET     MG  X 167       1                                                       
HET     MG  X 168       1                                                       
HET     MG  X 169       1                                                       
HET     MG  X 170       1                                                       
HET     MG  X 171       1                                                       
HET    TRS  X 199       8                                                       
HET    GNP  X 200      32                                                       
HET    XY2  X 332      21                                                       
HETNAM      MG MAGNESIUM ION                                                    
HETNAM     TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL                         
HETNAM     GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER                      
HETNAM     XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-                      
HETNAM   2 XY2  OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE                             
HETSYN     TRS TRIS BUFFER                                                      
FORMUL   2   MG    5(MG 2+)                                                     
FORMUL   3  TRS    C4 H12 N O3 1+                                               
FORMUL   4  GNP    C10 H17 N6 O13 P3                                            
FORMUL   5  XY2    C12 H15 N5 O4                                                
FORMUL   6  HOH   *168(H2 O)                                                    
HELIX    1   1 GLY X   15  ASN X   26  1                                  12    
HELIX    2   2 MET X   67  THR X   74  1                                   8    
HELIX    3   3 ASN X   86  ASP X   92  1                                   7    
HELIX    4   4 ASP X   92  LYS X  104  1                                  13    
HELIX    5   5 GLU X  126  GLY X  138  1                                  13    
HELIX    6   6 GLY X  151  ARG X  164  1                                  14    
SHEET    1  XA 6 GLU X  37  ILE X  46  0                                        
SHEET    2  XA 6 GLU X  49  THR X  58 -1  O  GLU X  49   N  ILE X  46           
SHEET    3  XA 6 THR X   2  GLY X  10  1  O  THR X   2   N  LEU X  52           
SHEET    4  XA 6 GLY X  77  ALA X  83  1  O  GLY X  77   N  VAL X   7           
SHEET    5  XA 6 MET X 111  ASN X 116  1  O  VAL X 112   N  CYS X  80           
SHEET    6  XA 6 TYR X 141  GLU X 143  1  O  ILE X 142   N  GLY X 115           
LINK         SG  CYS X  32                 C19 XY2 X 332     1555   1555  1.76  
LINK        MG    MG X 167                 O   HOH X2119     1555   3675  2.18  
LINK        MG    MG X 167                 O   HOH X2110     1555   1555  1.91  
LINK        MG    MG X 167                 O   HOH X2110     1555   3675  2.16  
LINK        MG    MG X 167                 O   HOH X2119     1555   2765  2.17  
LINK        MG    MG X 167                 O   HOH X2110     1555   2765  1.93  
LINK        MG    MG X 167                 O   HOH X2119     1555   1555  1.94  
LINK        MG    MG X 168                 O2B GNP X 200     1555   1555  2.03  
LINK        MG    MG X 168                 O   HOH X2067     1555   1555  2.03  
LINK        MG    MG X 168                 O1G GNP X 200     1555   1555  1.95  
LINK        MG    MG X 168                 O   HOH X2034     1555   1555  2.07  
LINK        MG    MG X 168                 O   HOH X2161     1555   1555  2.07  
LINK        MG    MG X 168                 OG  SER X  17     1555   1555  2.05  
LINK        MG    MG X 169                 O   HOH X2112     1555   1555  2.15  
LINK        MG    MG X 169                 O   HOH X2113     1555   1555  2.10  
LINK        MG    MG X 169                 O   HOH X2121     1555   3675  2.08  
LINK        MG    MG X 169                 O   HOH X2123     1555   3675  2.26  
LINK        MG    MG X 169                 O   HOH X2122     1555   3675  2.14  
LINK        MG    MG X 169                 OD1 ASP X 132     1555   3675  1.95  
LINK        MG    MG X 170                 O   HOH X2074     1555   1555  2.14  
LINK        MG    MG X 170                 OE1 GLU X  63     1555   1555  2.14  
LINK        MG    MG X 170                 O   HOH X2072     1555   1555  2.11  
LINK        MG    MG X 170                 O   HOH X2030     1555   1555  2.07  
LINK        MG    MG X 170                 O3G GNP X 200     1555   1555  2.03  
LINK        MG    MG X 170                 O   HOH X2035     1555   1555  2.08  
LINK        MG    MG X 171                 O   HOH X2132     1555  11565  2.30  
LINK        MG    MG X 171                 O   HOH X2156     1555   1555  2.58  
LINK        MG    MG X 171                 O   HOH X2156     1555  11565  1.49  
LINK        MG    MG X 171                 O   HOH X2132     1555   1555  1.96  
LINK        MG    MG X 171                 O   HOH X2041     1555  11565  2.27  
LINK        MG    MG X 171                 O   HOH X2041     1555   1555  2.02  
LINK        MG    MG X 171                 O   HOH X2155     1555  11565  2.14  
LINK        MG    MG X 171                 O   HOH X2155     1555   1555  2.14  
SITE     1 AC1  2 HOH X2110  HOH X2119                                          
SITE     1 AC2  5 SER X  17  GNP X 200  HOH X2034  HOH X2067                    
SITE     2 AC2  5 HOH X2161                                                     
SITE     1 AC3  6 ASP X 132  HOH X2112  HOH X2113  HOH X2121                    
SITE     2 AC3  6 HOH X2122  HOH X2123                                          
SITE     1 AC4  6 GLU X  63  GNP X 200  HOH X2030  HOH X2035                    
SITE     2 AC4  6 HOH X2072  HOH X2074                                          
SITE     1 AC5  5 GLN X 165  HOH X2041  HOH X2132  HOH X2155                    
SITE     2 AC5  5 HOH X2156                                                     
SITE     1 AC6  6 GLN X  25  ASP X  38  SER X  39  TYR X  40                    
SITE     2 AC6  6 HOH X2159  HOH X2160                                          
SITE     1 AC7 34 GLY X  12  GLY X  13  VAL X  14  GLY X  15                    
SITE     2 AC7 34 LYS X  16  SER X  17  ALA X  18  PHE X  28                    
SITE     3 AC7 34 VAL X  29  ASP X  30  GLU X  31  ASP X  33                    
SITE     4 AC7 34 GLY X  60  GLU X  63  ASN X 116  LYS X 117                    
SITE     5 AC7 34 ASP X 119  LEU X 120  SER X 145  ALA X 146                    
SITE     6 AC7 34 LYS X 147   MG X 168   MG X 170  XY2 X 332                    
SITE     7 AC7 34 HOH X2027  HOH X2030  HOH X2034  HOH X2035                    
SITE     8 AC7 34 HOH X2067  HOH X2072  HOH X2073  HOH X2161                    
SITE     9 AC7 34 HOH X2165  HOH X2166                                          
SITE     1 AC8  4 GLU X  31  CYS X  32  GNP X 200  HOH X2017                    
CRYST1   88.540   88.540  144.100  90.00  90.00 120.00 H 3 2        18          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.011294  0.006521  0.000000        0.00000                         
SCALE2      0.000000  0.013042  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.006940        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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