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Database: PDB
Entry: 2COP
LinkDB: 2COP
Original site: 2COP 
HEADER    LIPID BINDING PROTEIN                   18-MAY-05   2COP              
TITLE     SOLUTION STRUCTURE OF RSGI RUH-040, AN ACBP DOMAIN FROM               
TITLE    2 HUMAN CDNA                                                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ACYL-COENZYME A BINDING DOMAIN CONTAINING 6;               
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: ACYL COA BINDING DOMAIN, ACBP;                              
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: GENEID:84320;                                                  
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040830-11;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS (E.COLI)                  
KEYWDS    ACYL COA BINDING PROTEIN, COA BINDING PROTEIN, LIPID                  
KEYWDS   2 BINDING PROTEIN, STRUCTURAL GENOMICS, NPPSFA, NATIONAL               
KEYWDS   3 PROJECT ON PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES,               
KEYWDS   4 RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI                
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    A.Z.M.RUHUL MOMEN,H.HIROTA,F.HAYASHI,S.YOKOYAMA,RIKEN                 
AUTHOR   2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)                     
REVDAT   2   24-FEB-09 2COP    1       VERSN                                    
REVDAT   1   18-NOV-05 2COP    0                                                
JRNL        AUTH   A.Z.M.RUHUL MOMEN,H.HIROTA,F.HAYASHI,S.YOKOYAMA              
JRNL        TITL   SOLUTION STRUCTURE OF RSGI RUH-040, AN ACBP DOMAIN           
JRNL        TITL 2 FROM HUMAN CDNA                                              
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : NULL                                                 
REMARK   3   AUTHORS     : NULL                                                 
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2COP COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 24-MAY-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB024457.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM NACL                         
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.26MM ACBP DOMAIN U-15N,          
REMARK 210                                   13C; 20MM D-TRIS-HCL (PH7.0);      
REMARK 210                                   100MM NACL; 1MM D-DTT; 0.02%       
REMARK 210                                   NAN3; 10% D20                      
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1C, NMRPIPE 20031121,       
REMARK 210                                   NMRVIEW 5.0.4, KUJIRA 0.9295,      
REMARK 210                                   CYANA 2.0.17                       
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION,STRUCTURES         
REMARK 210                                   WITH THE LOWEST ENERGY,            
REMARK 210                                   STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   3      160.72    -44.49                                   
REMARK 500  1 VAL A  43      -65.19    -96.23                                   
REMARK 500  1 ASN A  47       35.26     35.19                                   
REMARK 500  1 ASP A  55       78.70    -68.82                                   
REMARK 500  1 ILE A  81      -65.34    -90.56                                   
REMARK 500  1 GLU A  97      167.54    -47.51                                   
REMARK 500  1 SER A 104      100.84    -51.43                                   
REMARK 500  1 PRO A 106     -175.04    -69.75                                   
REMARK 500  2 ARG A  28      -32.24    -39.53                                   
REMARK 500  2 VAL A  43      -63.90    -91.66                                   
REMARK 500  2 ASN A  45      106.55    -46.18                                   
REMARK 500  2 CYS A  46      129.03    -38.12                                   
REMARK 500  2 SER A  52      152.23    -48.23                                   
REMARK 500  2 GLU A  97      152.82    -46.33                                   
REMARK 500  2 GLU A 102       88.27    -52.17                                   
REMARK 500  3 SER A   2       94.68    -68.72                                   
REMARK 500  3 VAL A  43      -65.53    -95.18                                   
REMARK 500  3 GLU A  63      -71.76    -62.72                                   
REMARK 500  3 ILE A  81      -65.39    -91.63                                   
REMARK 500  3 LYS A  99      170.37    -51.99                                   
REMARK 500  3 LYS A 101       42.16   -109.08                                   
REMARK 500  3 ALA A 103      140.58   -172.76                                   
REMARK 500  3 PRO A 106        2.61    -69.75                                   
REMARK 500  4 VAL A  43      -64.65    -94.76                                   
REMARK 500  4 ASP A  70       51.12   -109.38                                   
REMARK 500  4 LYS A 101      113.15   -172.35                                   
REMARK 500  4 ALA A 103       42.07    -83.83                                   
REMARK 500  4 SER A 107       52.25    -93.63                                   
REMARK 500  4 SER A 108      -60.96   -125.17                                   
REMARK 500  5 GLU A  10      -39.80    -36.31                                   
REMARK 500  5 VAL A  43      -66.52    -99.00                                   
REMARK 500  5 ASN A  45      108.34    -54.03                                   
REMARK 500  5 CYS A  46      106.63    -36.33                                   
REMARK 500  5 PRO A  51     -177.49    -69.71                                   
REMARK 500  5 ASP A  55       79.73    -69.11                                   
REMARK 500  5 GLU A  63      -71.61    -61.45                                   
REMARK 500  5 LEU A  68       50.94    -90.34                                   
REMARK 500  5 GLU A  97      171.77    -49.46                                   
REMARK 500  6 GLU A  10      -36.48    -38.98                                   
REMARK 500  6 ARG A  28      -30.88    -37.50                                   
REMARK 500  6 VAL A  43      -69.86    -91.24                                   
REMARK 500  6 GLU A  63      -70.27    -61.18                                   
REMARK 500  6 ASP A  70       51.79   -103.82                                   
REMARK 500  6 GLU A  97      167.88    -45.14                                   
REMARK 500  6 GLU A 102      153.21    -44.06                                   
REMARK 500  6 ALA A 103       42.92    -82.87                                   
REMARK 500  6 SER A 104       83.60    -59.68                                   
REMARK 500  7 LEU A   8      -61.49    -90.19                                   
REMARK 500  7 VAL A  43      -64.97    -97.35                                   
REMARK 500  7 CYS A  46      105.30    -35.94                                   
REMARK 500  7 LEU A  68       51.44    -92.94                                   
REMARK 500  7 GLU A  97      166.58    -43.94                                   
REMARK 500  7 SER A 104      -52.98   -124.90                                   
REMARK 500  8 VAL A  43      -68.36    -92.25                                   
REMARK 500  8 CYS A  46      125.37    -38.49                                   
REMARK 500  8 ASP A  55       78.10    -69.42                                   
REMARK 500  8 LYS A 101       52.71    -99.09                                   
REMARK 500  8 ALA A 103       99.20    -55.15                                   
REMARK 500  9 GLU A  10      -38.92    -35.38                                   
REMARK 500  9 VAL A  43      -66.91   -102.10                                   
REMARK 500  9 ASP A  55       86.15    -59.79                                   
REMARK 500  9 GLN A  94      117.95   -170.18                                   
REMARK 500  9 GLU A  97      155.24    -46.02                                   
REMARK 500  9 LYS A 101       88.53    -64.51                                   
REMARK 500  9 ALA A 103      120.85    -38.09                                   
REMARK 500  9 SER A 107      103.06    -52.90                                   
REMARK 500 10 ARG A  28      -37.14    -39.08                                   
REMARK 500 10 VAL A  43      -65.56    -98.97                                   
REMARK 500 10 CYS A  46       95.43    -37.34                                   
REMARK 500 10 ASP A  55       80.53    -69.84                                   
REMARK 500 10 GLU A  63      -71.51    -64.09                                   
REMARK 500 10 LEU A  68       49.73    -88.72                                   
REMARK 500 10 ASP A  70       45.24   -102.48                                   
REMARK 500 10 GLU A  97      171.21    -47.96                                   
REMARK 500 10 LYS A  98       43.34    -92.02                                   
REMARK 500 10 ALA A 103      114.30    -37.60                                   
REMARK 500 10 SER A 108      107.04   -165.32                                   
REMARK 500 11 SER A   6      103.22    -35.45                                   
REMARK 500 11 VAL A  43      -66.34    -90.01                                   
REMARK 500 11 CYS A  46      126.16    -39.35                                   
REMARK 500 11 GLU A  97      154.08    -44.04                                   
REMARK 500 11 PRO A 106     -173.39    -69.74                                   
REMARK 500 12 SER A   3      121.35    -36.51                                   
REMARK 500 12 GLU A  97      150.19    -41.42                                   
REMARK 500 12 LYS A  99       48.48     36.72                                   
REMARK 500 13 SER A   3      -48.42   -130.90                                   
REMARK 500 13 PHE A  12      -36.56    -36.42                                   
REMARK 500 13 ASP A  55       83.49    -67.70                                   
REMARK 500 13 ASP A  70       51.17   -112.23                                   
REMARK 500 13 PRO A  89        2.82    -69.67                                   
REMARK 500 13 GLU A  97      156.96    -46.90                                   
REMARK 500 14 SER A   3       78.96   -117.49                                   
REMARK 500 14 PHE A  12      -36.09    -34.28                                   
REMARK 500 14 VAL A  43      -65.32    -94.95                                   
REMARK 500 14 CYS A  46       97.12    -56.94                                   
REMARK 500 14 ASP A  55       80.92    -67.53                                   
REMARK 500 14 LEU A  68       51.20    -92.82                                   
REMARK 500 14 ASP A  70       40.35   -109.09                                   
REMARK 500 14 GLU A 102       28.61     47.43                                   
REMARK 500 14 SER A 107      137.24    -35.52                                   
REMARK 500 15 SER A   5       48.21     36.90                                   
REMARK 500 15 GLU A  10      -39.39    -35.63                                   
REMARK 500 15 CYS A  46       85.80    -53.89                                   
REMARK 500 15 ASN A  47       30.87    -99.48                                   
REMARK 500 15 GLU A  63      -71.40    -60.91                                   
REMARK 500 15 ASP A  70       52.07   -104.33                                   
REMARK 500 15 GLU A  97      167.69    -49.91                                   
REMARK 500 15 PRO A 106        1.22    -69.72                                   
REMARK 500 15 SER A 107      151.89    -37.28                                   
REMARK 500 16 SER A   6      124.04   -174.82                                   
REMARK 500 16 VAL A  43      -60.33    -91.03                                   
REMARK 500 16 CYS A  46      119.88    -35.92                                   
REMARK 500 16 ASP A  55       86.54    -58.46                                   
REMARK 500 16 ASP A  70       51.35   -106.63                                   
REMARK 500 16 LYS A  99       35.65    -85.95                                   
REMARK 500 16 PRO A 106       91.95    -69.80                                   
REMARK 500 17 SER A   2      170.61    -49.72                                   
REMARK 500 17 ARG A  28      -35.85    -38.03                                   
REMARK 500 17 ALA A  36      -71.53    -40.62                                   
REMARK 500 17 VAL A  43      -70.33    -91.18                                   
REMARK 500 17 CYS A  46       95.90    -47.11                                   
REMARK 500 17 ASP A  55       75.79    -69.14                                   
REMARK 500 17 PRO A  89        1.75    -69.78                                   
REMARK 500 17 LYS A  99      134.60   -174.98                                   
REMARK 500 17 PRO A 106        2.73    -69.71                                   
REMARK 500 17 SER A 107      136.00    -35.52                                   
REMARK 500 18 SER A   2      -52.94   -120.25                                   
REMARK 500 18 SER A   6       41.43     38.26                                   
REMARK 500 18 CYS A  46      112.28    -38.18                                   
REMARK 500 18 GLU A  63      -70.84    -58.83                                   
REMARK 500 18 LEU A  68       50.44    -91.69                                   
REMARK 500 18 ALA A 103       94.55    -57.39                                   
REMARK 500 18 SER A 104       35.22     73.38                                   
REMARK 500 19 PHE A  12      -37.97    -39.51                                   
REMARK 500 19 THR A  48      154.55    -40.83                                   
REMARK 500 19 GLU A  63      -71.54    -54.08                                   
REMARK 500 19 ASP A  70       54.27   -109.65                                   
REMARK 500 19 SER A 108      114.09   -168.52                                   
REMARK 500 20 GLU A  10      -39.73    -36.59                                   
REMARK 500 20 ARG A  28      -30.37    -36.16                                   
REMARK 500 20 VAL A  43      -66.58    -96.46                                   
REMARK 500 20 CYS A  46      124.79    -35.93                                   
REMARK 500 20 ASP A  55       82.15    -64.29                                   
REMARK 500 20 LEU A  68       52.70    -93.24                                   
REMARK 500 20 ILE A  81      -66.63    -90.73                                   
REMARK 500 20 PRO A  89        0.86    -69.77                                   
REMARK 500 20 GLU A  97      162.62    -47.67                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSI002006003.1   RELATED DB: TARGETDB                    
DBREF  2COP A    8   103  UNP    Q9BR61   Q9BR61_HUMAN    42    137             
SEQADV 2COP GLY A    1  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A    2  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A    3  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP GLY A    4  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A    5  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A    6  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP GLY A    7  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A  104  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP GLY A  105  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP PRO A  106  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A  107  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP SER A  108  UNP  Q9BR61              CLONING ARTIFACT               
SEQADV 2COP GLY A  109  UNP  Q9BR61              CLONING ARTIFACT               
SEQRES   1 A  109  GLY SER SER GLY SER SER GLY LEU ALA GLU LEU PHE GLU          
SEQRES   2 A  109  LYS ALA ALA ALA HIS LEU GLN GLY LEU ILE GLN VAL ALA          
SEQRES   3 A  109  SER ARG GLU GLN LEU LEU TYR LEU TYR ALA ARG TYR LYS          
SEQRES   4 A  109  GLN VAL LYS VAL GLY ASN CYS ASN THR PRO LYS PRO SER          
SEQRES   5 A  109  PHE PHE ASP PHE GLU GLY LYS GLN LYS TRP GLU ALA TRP          
SEQRES   6 A  109  LYS ALA LEU GLY ASP SER SER PRO SER GLN ALA MET GLN          
SEQRES   7 A  109  GLU TYR ILE ALA VAL VAL LYS LYS LEU ASP PRO GLY TRP          
SEQRES   8 A  109  ASN PRO GLN ILE PRO GLU LYS LYS GLY LYS GLU ALA SER          
SEQRES   9 A  109  GLY PRO SER SER GLY                                          
HELIX    1   1 LEU A    8  GLN A   20  1                                  13    
HELIX    2   2 SER A   27  VAL A   43  1                                  17    
HELIX    3   3 ASP A   55  ALA A   67  1                                  13    
HELIX    4   4 SER A   72  ASP A   88  1                                  17    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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