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Database: PDB
Entry: 2CQ0
LinkDB: 2CQ0
Original site: 2CQ0 
HEADER    TRANSCRIPTION                           19-MAY-05   2CQ0              
TITLE     SOLUTION STRUCTURE OF RNA BINDING DOMAIN IN EUKARYOTIC                
TITLE    2 TRANSLATION INITIATION FACTOR 3 SUBUNIT 4                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: EUKARYOTIC TRANSLATION INITIATION FACTOR 3                 
COMPND   3 SUBUNIT 4;                                                           
COMPND   4 CHAIN: A;                                                            
COMPND   5 FRAGMENT: RNA RECOGNITION MOTIF;                                     
COMPND   6 SYNONYM: EIF-3 DELTA, EIF3 P44, EIF-3 RNA-BINDING SUBUNIT,           
COMPND   7 EIF3 P42, EIF3G;                                                     
COMPND   8 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: EIF3S4;                                                        
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040510-15;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHEIS                            
KEYWDS    RRM DOMAIN, STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT             
KEYWDS   2 ON PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN                 
KEYWDS   3 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI,                     
KEYWDS   4 TRANSCRIPTION                                                        
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.TSUDA,Y.MUTO,M.INOUE,T.KIGAWA,T.TERADA,M.SHIROUZU,                  
AUTHOR   2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE           
AUTHOR   3 (RSGI)                                                               
REVDAT   2   24-FEB-09 2CQ0    1       VERSN                                    
REVDAT   1   19-NOV-05 2CQ0    0                                                
JRNL        AUTH   K.TSUDA,Y.MUTO,M.INOUE,T.KIGAWA,T.TERADA,                    
JRNL        AUTH 2 M.SHIROUZU,S.YOKOYAMA                                        
JRNL        TITL   SOLUTION STRUCTURE OF RNA BINDING DOMAIN IN                  
JRNL        TITL 2 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT           
JRNL        TITL 3 4                                                            
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT, P                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2CQ0 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 24-MAY-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB024491.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.15MM 13C/15N-PROTEIN, 20MM       
REMARK 210                                   D-TRIS-HCL (PH 7.0), 100MM         
REMARK 210                                   NACL, 1MM D-DTT, 0.02% NAN3,       
REMARK 210                                   10% D2O                            
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 3.5, NMRPIPE               
REMARK 210                                   20030801, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.9297, CYANA 2.0.17        
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS,            
REMARK 210                                   RESTRAINTED MOLECULAR DYNAMICS     
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION, STRUCTURES        
REMARK 210                                   WITH THE LOWEST ENERGY,            
REMARK 210                                   STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 PRO A 231      101.44    -45.76                                   
REMARK 500  1 ASN A 232       97.67    -57.59                                   
REMARK 500  1 ASP A 237      -45.24   -134.03                                   
REMARK 500  1 ASN A 238      152.26    -48.11                                   
REMARK 500  1 ASN A 245       52.07     70.45                                   
REMARK 500  1 PRO A 261      -17.23    -48.02                                   
REMARK 500  1 ILE A 297      -33.72    -37.97                                   
REMARK 500  1 SER A 318       45.23    -93.21                                   
REMARK 500  2 PRO A 261      -17.14    -48.00                                   
REMARK 500  2 LYS A 280      -73.77    -46.87                                   
REMARK 500  2 ILE A 297      -35.16    -39.94                                   
REMARK 500  2 SER A 301      104.47    -59.81                                   
REMARK 500  3 SER A 229      112.76    -35.75                                   
REMARK 500  3 ASN A 232       42.93   -100.52                                   
REMARK 500  3 ALA A 235      106.82   -161.97                                   
REMARK 500  3 ASP A 237      -71.33   -121.31                                   
REMARK 500  3 ASN A 245       38.41     70.79                                   
REMARK 500  3 PRO A 261      -17.21    -48.01                                   
REMARK 500  3 LYS A 280      -75.00    -68.77                                   
REMARK 500  3 ILE A 297      -33.20    -35.04                                   
REMARK 500  3 LYS A 316      150.35    -41.61                                   
REMARK 500  3 PRO A 317       44.59    -77.07                                   
REMARK 500  3 SER A 318      108.18    -44.49                                   
REMARK 500  4 PRO A 261      -17.24    -47.96                                   
REMARK 500  4 ILE A 297      -34.65    -39.86                                   
REMARK 500  4 HIS A 307       27.25     45.91                                   
REMARK 500  4 PRO A 317      156.74    -47.01                                   
REMARK 500  5 ASN A 232      -61.85    -92.56                                   
REMARK 500  5 ASN A 245       38.43     73.58                                   
REMARK 500  5 PRO A 261      -18.29    -47.98                                   
REMARK 500  5 LYS A 280      -72.90    -63.58                                   
REMARK 500  5 ALA A 296      -70.51    -64.50                                   
REMARK 500  5 ILE A 297      -34.72    -35.08                                   
REMARK 500  5 HIS A 307       29.98     47.62                                   
REMARK 500  5 SER A 318      -53.38   -129.17                                   
REMARK 500  5 SER A 321      127.35    -39.90                                   
REMARK 500  6 ASN A 238      159.84    -39.85                                   
REMARK 500  6 ASN A 245       39.06     73.32                                   
REMARK 500  6 PRO A 261      -17.23    -47.93                                   
REMARK 500  6 ASP A 273     -178.61    -68.49                                   
REMARK 500  6 ILE A 297      -35.95    -35.88                                   
REMARK 500  7 PRO A 231      166.99    -45.51                                   
REMARK 500  7 ASP A 237       38.79     35.19                                   
REMARK 500  7 ASN A 245       43.77     71.59                                   
REMARK 500  7 PRO A 261      -17.93    -47.97                                   
REMARK 500  7 THR A 275      -72.38    -55.14                                   
REMARK 500  7 ILE A 297      -32.97    -34.48                                   
REMARK 500  8 SER A 228      171.26    -46.97                                   
REMARK 500  8 ARG A 234      -62.21   -127.69                                   
REMARK 500  8 ALA A 235      -38.84    -35.40                                   
REMARK 500  8 PRO A 261      -17.18    -47.94                                   
REMARK 500  8 ILE A 297      -34.12    -36.91                                   
REMARK 500  8 ALA A 315      -71.31    -69.89                                   
REMARK 500  8 SER A 321      -48.65   -131.20                                   
REMARK 500  8 SER A 324      152.38    -45.74                                   
REMARK 500  9 SER A 226       89.81    -56.12                                   
REMARK 500  9 SER A 229      174.66    -59.85                                   
REMARK 500  9 ASP A 237       37.86     34.51                                   
REMARK 500  9 PRO A 261      -17.37    -47.94                                   
REMARK 500  9 ILE A 297      -33.52    -35.97                                   
REMARK 500  9 HIS A 307       26.60     47.40                                   
REMARK 500  9 THR A 319       40.01     36.10                                   
REMARK 500 10 SER A 228      106.87    -40.19                                   
REMARK 500 10 PRO A 261      -17.16    -47.96                                   
REMARK 500 10 SER A 279      128.01    -36.53                                   
REMARK 500 10 LYS A 280      -74.33    -57.95                                   
REMARK 500 10 ILE A 297      -32.38    -35.71                                   
REMARK 500 11 ASP A 237       90.46    -65.87                                   
REMARK 500 11 ASN A 245       38.28     74.05                                   
REMARK 500 11 PRO A 261      -17.34    -47.88                                   
REMARK 500 11 ASP A 273     -177.87    -67.29                                   
REMARK 500 11 LYS A 280      -74.78    -62.13                                   
REMARK 500 11 ILE A 297      -32.22    -38.31                                   
REMARK 500 11 SER A 324       99.04    -59.20                                   
REMARK 500 12 SER A 226       46.91     36.30                                   
REMARK 500 12 ASP A 236      134.16    -38.90                                   
REMARK 500 12 ASP A 237      -61.58   -123.04                                   
REMARK 500 12 ASN A 245       48.65     74.53                                   
REMARK 500 12 PRO A 261      -17.59    -47.93                                   
REMARK 500 12 LYS A 316      105.01   -174.84                                   
REMARK 500 13 ARG A 234       51.07     35.44                                   
REMARK 500 13 PRO A 261      -18.57    -47.93                                   
REMARK 500 13 THR A 275      -72.49    -52.49                                   
REMARK 500 13 LYS A 280      -74.94    -63.38                                   
REMARK 500 13 ILE A 297      -34.52    -38.20                                   
REMARK 500 13 HIS A 307       26.98     48.74                                   
REMARK 500 14 SER A 226      -50.69   -122.99                                   
REMARK 500 14 ASP A 237      -54.88   -120.06                                   
REMARK 500 14 ASN A 245       41.96     73.13                                   
REMARK 500 14 PRO A 261      -17.42    -47.87                                   
REMARK 500 14 LYS A 280      -74.90    -82.05                                   
REMARK 500 14 ILE A 297      -37.01    -39.92                                   
REMARK 500 15 ARG A 234       47.36    -89.23                                   
REMARK 500 15 ASN A 245       43.94     73.59                                   
REMARK 500 15 LYS A 280      -74.86    -55.10                                   
REMARK 500 15 ILE A 297      -32.42    -36.03                                   
REMARK 500 15 PRO A 317      103.40    -44.41                                   
REMARK 500 16 ASN A 232       50.91     37.35                                   
REMARK 500 16 PRO A 261      -17.98    -47.91                                   
REMARK 500 16 LYS A 280      -74.66    -66.68                                   
REMARK 500 16 ILE A 297      -37.73    -35.42                                   
REMARK 500 16 HIS A 307       26.61     48.80                                   
REMARK 500 16 SER A 318      141.86    -35.63                                   
REMARK 500 17 ASN A 245       43.36     72.28                                   
REMARK 500 17 PRO A 261      -17.60    -47.93                                   
REMARK 500 17 ALA A 296      -71.06    -60.75                                   
REMARK 500 17 SER A 301      104.47    -48.90                                   
REMARK 500 17 HIS A 307       31.31     37.91                                   
REMARK 500 17 SER A 318       48.80    -86.52                                   
REMARK 500 17 THR A 319       41.54    -81.95                                   
REMARK 500 17 PRO A 323      101.75    -45.56                                   
REMARK 500 18 ASP A 236      114.36   -168.61                                   
REMARK 500 18 ASP A 237      -60.61   -100.74                                   
REMARK 500 18 ASN A 245       41.90     72.32                                   
REMARK 500 18 PRO A 261      -19.12    -47.94                                   
REMARK 500 18 ASP A 273     -176.30    -55.42                                   
REMARK 500 18 THR A 275      -73.59    -67.08                                   
REMARK 500 18 ALA A 296      -70.91    -64.22                                   
REMARK 500 18 SER A 301      108.47    -47.92                                   
REMARK 500 18 HIS A 307       30.94     38.23                                   
REMARK 500 18 PRO A 317      161.66    -44.81                                   
REMARK 500 19 ASN A 245       49.78     73.77                                   
REMARK 500 19 PRO A 261      -17.55    -48.03                                   
REMARK 500 19 THR A 275      -73.94    -74.67                                   
REMARK 500 19 LYS A 280      -74.38    -61.47                                   
REMARK 500 19 ILE A 297      -34.16    -34.30                                   
REMARK 500 20 ARG A 234      109.63   -162.64                                   
REMARK 500 20 PRO A 261      -18.01    -48.00                                   
REMARK 500 20 ASP A 273     -179.92    -61.64                                   
REMARK 500 20 ALA A 296      -71.01    -64.43                                   
REMARK 500 20 HIS A 307       31.32     37.73                                   
REMARK 500 20 PRO A 317      153.89    -45.54                                   
REMARK 500 20 ASN A 320       47.18    -80.33                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSS001003366   RELATED DB: TARGETDB                      
DBREF  2CQ0 A  231   320  UNP    O75821   IF34_HUMAN     231    320             
SEQADV 2CQ0 GLY A  224  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  225  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  226  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 GLY A  227  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  228  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  229  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 GLY A  230  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  321  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 GLY A  322  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 PRO A  323  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  324  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 SER A  325  UNP  O75821              CLONING ARTIFACT               
SEQADV 2CQ0 GLY A  326  UNP  O75821              CLONING ARTIFACT               
SEQRES   1 A  103  GLY SER SER GLY SER SER GLY PRO ASN ARG ARG ALA ASP          
SEQRES   2 A  103  ASP ASN ALA THR ILE ARG VAL THR ASN LEU SER GLU ASP          
SEQRES   3 A  103  THR ARG GLU THR ASP LEU GLN GLU LEU PHE ARG PRO PHE          
SEQRES   4 A  103  GLY SER ILE SER ARG ILE TYR LEU ALA LYS ASP LYS THR          
SEQRES   5 A  103  THR GLY GLN SER LYS GLY PHE ALA PHE ILE SER PHE HIS          
SEQRES   6 A  103  ARG ARG GLU ASP ALA ALA ARG ALA ILE ALA GLY VAL SER          
SEQRES   7 A  103  GLY PHE GLY TYR ASP HIS LEU ILE LEU ASN VAL GLU TRP          
SEQRES   8 A  103  ALA LYS PRO SER THR ASN SER GLY PRO SER SER GLY              
HELIX    1   1 ARG A  251  GLU A  257  1                                   7    
HELIX    2   2 ARG A  289  VAL A  300  1                                  12    
SHEET    1   A 4 ILE A 265  LYS A 272  0                                        
SHEET    2   A 4 SER A 279  PHE A 287 -1  O  SER A 286   N  ARG A 267           
SHEET    3   A 4 ALA A 239  THR A 244 -1  N  VAL A 243   O  ALA A 283           
SHEET    4   A 4 ASN A 311  TRP A 314 -1  O  GLU A 313   N  ARG A 242           
SHEET    1   B 2 GLY A 304  TYR A 305  0                                        
SHEET    2   B 2 LEU A 308  ILE A 309 -1  O  LEU A 308   N  TYR A 305           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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