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Database: PDB
Entry: 2CQH
LinkDB: 2CQH
Original site: 2CQH 
HEADER    RNA BINDING PROTEIN                     20-MAY-05   2CQH              
TITLE     SOLUTION STRUCTURE OF THE RNA BINDING DOMAIN OF IGF-II MRNA-          
TITLE    2 BINDING PROTEIN 2                                                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: IGF-II MRNA-BINDING PROTEIN 2 ISOFORM A;                   
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: RNA RECOGNITION MOTIF;                                     
COMPND   5 SYNONYM: INSULIN-LIKE GROWTH FACTOR 2 MRNA-BINDING PROTEIN           
COMPND   6 2;                                                                   
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: IMP2;                                                          
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P041108-02;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    RNA RECOGNITION MOTIF, RRM, RNA BINDING DOMAIN, RBD, RNP,             
KEYWDS   2 STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT ON PROTEIN             
KEYWDS   3 STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL                 
KEYWDS   4 GENOMICS/PROTEOMICS INITIATIVE, RSGI, RNA BINDING PROTEIN            
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    S.SUZUKI,Y.MUTO,M.INOUE,T.KIGAWA,T.TERADA,M.SHIROUZU,                 
AUTHOR   2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE           
AUTHOR   3 (RSGI)                                                               
REVDAT   2   24-FEB-09 2CQH    1       VERSN                                    
REVDAT   1   20-NOV-05 2CQH    0                                                
JRNL        AUTH   S.SUZUKI,Y.MUTO,M.INOUE,T.KIGAWA,T.TERADA,                   
JRNL        AUTH 2 M.SHIROUZU,S.YOKOYAMA                                        
JRNL        TITL   SOLUTION STRUCTURE OF THE RNA BINDING DOMAIN OF              
JRNL        TITL 2 IGF-II MRNA-BINDING PROTEIN 2                                
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2CQH COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 25-MAY-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB024508.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 293                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.2MM 13C/15N-PROTEIN; 20MM D      
REMARK 210                                   -TRIS-HCL(PH7.0); 100MM NACL;      
REMARK 210                                   1MM D-DTT; 0.02% NAN3              
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 700 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 3.5, NMRPIPE               
REMARK 210                                   20031121, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.9295, OLIVIA 1.10.5,      
REMARK 210                                   CYANA 2.0.17                       
REMARK 210   METHOD USED                   : TORSION ANGLE DYANAMICS,           
REMARK 210                                   SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION                    
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR      
REMARK 210  SPECTROSCOPY.                                                       
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   0      150.45    -38.59                                   
REMARK 500  1 ASN A   9       41.16     70.83                                   
REMARK 500  1 PRO A  12        0.00    -69.78                                   
REMARK 500  1 ALA A  31       30.70    -89.68                                   
REMARK 500  1 SER A  38       92.02    -67.60                                   
REMARK 500  1 LEU A  58      -71.71   -100.35                                   
REMARK 500  1 SER A  74       86.22    -68.62                                   
REMARK 500  1 SER A  76      -60.05   -122.08                                   
REMARK 500  1 LYS A  77       43.40     39.76                                   
REMARK 500  1 PRO A  84     -172.08    -69.77                                   
REMARK 500  2 SER A  11      143.61    -39.91                                   
REMARK 500  2 PRO A  12        2.89    -69.70                                   
REMARK 500  2 SER A  38       92.27    -67.91                                   
REMARK 500  2 LEU A  58      -73.29    -97.18                                   
REMARK 500  3 MET A   2      150.71    -43.64                                   
REMARK 500  3 ASN A   9       41.22     71.49                                   
REMARK 500  3 ALA A  31       34.44    -85.41                                   
REMARK 500  3 SER A  38       92.66    -67.95                                   
REMARK 500  3 LEU A  58      -67.66    -99.69                                   
REMARK 500  3 TYR A  73      153.75    -38.69                                   
REMARK 500  3 LYS A  77      107.55   -168.79                                   
REMARK 500  4 ASN A   9       42.96     70.38                                   
REMARK 500  4 SER A  11      151.19    -41.85                                   
REMARK 500  4 PRO A  12        0.14    -69.71                                   
REMARK 500  4 ALA A  31       37.31    -84.49                                   
REMARK 500  4 SER A  38       92.38    -67.78                                   
REMARK 500  4 LEU A  58      -74.23   -102.27                                   
REMARK 500  4 TYR A  73      152.42    -40.71                                   
REMARK 500  5 SER A  -4      -61.90   -125.71                                   
REMARK 500  5 SER A  -3       96.49    -57.25                                   
REMARK 500  5 PRO A  12        0.29    -69.83                                   
REMARK 500  5 LEU A  30      100.40    -58.23                                   
REMARK 500  5 ALA A  31       36.08    -83.46                                   
REMARK 500  5 SER A  38       91.36    -68.36                                   
REMARK 500  5 LEU A  58      -67.86   -101.61                                   
REMARK 500  5 TYR A  73      148.28    -37.68                                   
REMARK 500  6 ASN A   3      107.92    -52.19                                   
REMARK 500  6 ASN A   9       35.67     73.90                                   
REMARK 500  6 ALA A  31       34.49    -94.74                                   
REMARK 500  6 SER A  38       91.08    -68.28                                   
REMARK 500  6 LEU A  58      -67.08    -95.19                                   
REMARK 500  6 SER A  74       44.03   -101.95                                   
REMARK 500  7 SER A  -4      109.66   -170.04                                   
REMARK 500  7 SER A  11      150.95    -46.27                                   
REMARK 500  7 LEU A  28       77.82   -113.21                                   
REMARK 500  7 ALA A  31       31.36    -84.42                                   
REMARK 500  7 SER A  38       96.14    -67.24                                   
REMARK 500  7 LEU A  58      -72.70   -104.27                                   
REMARK 500  7 TYR A  73      144.63    -34.49                                   
REMARK 500  7 LYS A  77      176.52    -51.29                                   
REMARK 500  7 ARG A  80       89.61    -66.05                                   
REMARK 500  8 ASN A   9       46.47     73.08                                   
REMARK 500  8 LEU A  28       78.71   -115.94                                   
REMARK 500  8 SER A  38       86.90    -69.16                                   
REMARK 500  8 LEU A  58      -72.07   -101.16                                   
REMARK 500  9 SER A  -3      108.08    -54.51                                   
REMARK 500  9 ASN A   3      107.99    -54.54                                   
REMARK 500  9 ASN A   9       41.35     70.83                                   
REMARK 500  9 ALA A  31       35.11    -86.75                                   
REMARK 500  9 SER A  38       94.99    -67.03                                   
REMARK 500  9 LEU A  58      -71.70    -90.78                                   
REMARK 500  9 SER A  74       56.22    -96.18                                   
REMARK 500  9 LEU A  79      108.03    -57.42                                   
REMARK 500 10 ASN A   9       40.82     72.30                                   
REMARK 500 10 PRO A  12        0.56    -69.71                                   
REMARK 500 10 ALA A  31       38.52    -83.00                                   
REMARK 500 10 SER A  38       94.74    -67.31                                   
REMARK 500 10 LEU A  58      -69.07    -96.71                                   
REMARK 500 10 SER A  74       81.75    -65.26                                   
REMARK 500 10 PRO A  84     -177.89    -69.85                                   
REMARK 500 11 SER A  -4       41.66     38.36                                   
REMARK 500 11 SER A  -1      125.87    -37.80                                   
REMARK 500 11 SER A   0      142.44    -36.16                                   
REMARK 500 11 ALA A  31       36.28    -87.58                                   
REMARK 500 11 SER A  38       94.62    -67.42                                   
REMARK 500 11 LEU A  58      -75.05   -103.84                                   
REMARK 500 11 TYR A  73      132.67    -36.20                                   
REMARK 500 11 SER A  74       48.11    -85.04                                   
REMARK 500 12 SER A  -1       42.48   -106.03                                   
REMARK 500 12 SER A  11      154.92    -47.77                                   
REMARK 500 12 ALA A  31       37.29    -82.63                                   
REMARK 500 12 SER A  38       90.73    -68.00                                   
REMARK 500 12 LEU A  58      -70.26   -102.43                                   
REMARK 500 12 TYR A  73      148.31    -35.42                                   
REMARK 500 12 SER A  74       42.37   -100.31                                   
REMARK 500 12 SER A  81      137.17   -173.54                                   
REMARK 500 13 PRO A  12        2.80    -69.74                                   
REMARK 500 13 LEU A  30      100.24    -57.40                                   
REMARK 500 13 ALA A  31       41.11    -86.21                                   
REMARK 500 13 SER A  38       90.29    -68.31                                   
REMARK 500 13 LEU A  58      -70.90    -99.86                                   
REMARK 500 13 TYR A  73      136.59    -34.54                                   
REMARK 500 13 SER A  74       41.34    -94.96                                   
REMARK 500 13 VAL A  75       39.68    -84.04                                   
REMARK 500 14 ASN A   9       45.04     71.98                                   
REMARK 500 14 PRO A  12        0.29    -69.70                                   
REMARK 500 14 SER A  38       89.76    -69.21                                   
REMARK 500 14 LEU A  58      -68.74    -97.49                                   
REMARK 500 14 SER A  59      108.69    -53.65                                   
REMARK 500 14 TYR A  73      123.69    -39.15                                   
REMARK 500 14 LYS A  77      132.78   -171.83                                   
REMARK 500 14 ARG A  80      113.76   -163.19                                   
REMARK 500 15 SER A  38       93.17    -67.00                                   
REMARK 500 15 LEU A  58      -73.45   -100.18                                   
REMARK 500 15 TYR A  73      153.79    -44.15                                   
REMARK 500 15 SER A  74       53.48    -94.42                                   
REMARK 500 15 LYS A  77      119.07   -171.50                                   
REMARK 500 15 ARG A  80      152.24    -40.14                                   
REMARK 500 16 ASN A   9       50.11     74.82                                   
REMARK 500 16 SER A  11      150.46    -44.14                                   
REMARK 500 16 ALA A  31       39.85    -87.06                                   
REMARK 500 16 SER A  38       89.36    -68.78                                   
REMARK 500 16 LEU A  58      -71.96   -100.37                                   
REMARK 500 16 SER A  81      100.96    -36.54                                   
REMARK 500 17 SER A  11      151.58    -45.67                                   
REMARK 500 17 ALA A  31       42.08    -86.46                                   
REMARK 500 17 SER A  38       90.14    -68.34                                   
REMARK 500 17 LEU A  58      -71.57    -97.63                                   
REMARK 500 17 VAL A  75       46.08     33.82                                   
REMARK 500 17 SER A  76       47.17    -84.40                                   
REMARK 500 18 SER A  -1       97.25    -60.79                                   
REMARK 500 18 ASN A   9       41.14     71.08                                   
REMARK 500 18 PRO A  12        2.02    -69.70                                   
REMARK 500 18 ALA A  31       31.10    -97.53                                   
REMARK 500 18 SER A  38       88.61    -69.17                                   
REMARK 500 18 LEU A  58      -74.48    -94.26                                   
REMARK 500 18 TYR A  73      139.99    -35.67                                   
REMARK 500 18 SER A  85      108.87    -50.44                                   
REMARK 500 18 SER A  86      106.65    -55.30                                   
REMARK 500 19 SER A   0       93.60    -57.63                                   
REMARK 500 19 ASN A   9       44.87     72.86                                   
REMARK 500 19 SER A  11      152.76    -48.89                                   
REMARK 500 19 PRO A  12        0.74    -69.82                                   
REMARK 500 19 ALA A  31       34.16    -93.09                                   
REMARK 500 19 SER A  38       92.17    -67.87                                   
REMARK 500 19 LEU A  58      -71.15    -97.42                                   
REMARK 500 19 TYR A  73      126.73    -35.69                                   
REMARK 500 19 SER A  76      -70.13   -125.42                                   
REMARK 500 19 LYS A  77      106.22   -173.96                                   
REMARK 500 19 LYS A  78      137.05    -36.34                                   
REMARK 500 19 SER A  82      -71.73   -101.52                                   
REMARK 500 20 MET A   2      128.28    -37.43                                   
REMARK 500 20 SER A  11      151.54    -49.89                                   
REMARK 500 20 ALA A  31       32.36    -95.52                                   
REMARK 500 20 SER A  38       84.81    -69.51                                   
REMARK 500 20 LEU A  58      -74.07    -96.59                                   
REMARK 500 20 TYR A  73      139.71    -36.59                                   
REMARK 500 20 SER A  74       58.86    -93.27                                   
REMARK 500 20 LYS A  77      138.44   -172.24                                   
REMARK 500 20 SER A  86      137.55   -170.59                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650 HELIX                                                                
REMARK 650 DETERMINATION METHOD: AUTHOR DETERMINED                              
REMARK 700                                                                      
REMARK 700 SHEET                                                                
REMARK 700 DETERMINATION METHOD: AUTHOR DETERMINED                              
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSI002013968.1   RELATED DB: TARGETDB                    
DBREF  2CQH A    2    81  UNP    Q9Y6M1   Q9Y6M1_HUMAN     2     81             
SEQADV 2CQH GLY A   -5  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A   -4  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A   -3  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH GLY A   -2  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A   -1  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A    0  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH GLY A    1  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A   82  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH GLY A   83  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH PRO A   84  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A   85  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH SER A   86  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQADV 2CQH GLY A   87  UNP  Q9Y6M1              CLONING ARTIFACT               
SEQRES   1 A   93  GLY SER SER GLY SER SER GLY MET ASN LYS LEU TYR ILE          
SEQRES   2 A   93  GLY ASN LEU SER PRO ALA VAL THR ALA ASP ASP LEU ARG          
SEQRES   3 A   93  GLN LEU PHE GLY ASP ARG LYS LEU PRO LEU ALA GLY GLN          
SEQRES   4 A   93  VAL LEU LEU LYS SER GLY TYR ALA PHE VAL ASP TYR PRO          
SEQRES   5 A   93  ASP GLN ASN TRP ALA ILE ARG ALA ILE GLU THR LEU SER          
SEQRES   6 A   93  GLY LYS VAL GLU LEU HIS GLY LYS ILE MET GLU VAL ASP          
SEQRES   7 A   93  TYR SER VAL SER LYS LYS LEU ARG SER SER GLY PRO SER          
SEQRES   8 A   93  SER GLY                                                      
HELIX    1   1 ALA A   16  ARG A   26  1                                  11    
HELIX    2   2 GLN A   48  THR A   57  1                                  10    
SHEET    1   A 4 LYS A   4  GLY A   8  0                                        
SHEET    2   A 4 GLN A  33  LYS A  37 -1                                        
SHEET    3   A 4 TYR A  40  ASP A  44 -1                                        
SHEET    4   A 4 GLU A  63  LEU A  64 -1                                        
SHEET    1   B 2 LYS A  67  ILE A  68  0                                        
SHEET    2   B 2 GLU A  70  TYR A  73 -1                                        
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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