HEADER TRANSPORT PROTEIN 20-MAY-05 2CR0
TITLE SOLUTION STRUCTURE OF NUCLEAR MOVE DOMAIN OF NUCLEAR DISTRIBUTION GENE
TITLE 2 C
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: NUCLEAR MIGRATION PROTEIN NUDC;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: NUCLEAR MOVE;
COMPND 5 SYNONYM: NUCLEAR DISTRIBUTION PROTEIN C HOMOLOG, SILICA-INDUCED GENE
COMPND 6 92 PROTEIN, SIG-92;
COMPND 7 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;
SOURCE 3 ORGANISM_COMMON: HOUSE MOUSE;
SOURCE 4 ORGANISM_TAXID: 10090;
SOURCE 5 GENE: RIKEN CDNA 2700033I24;
SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: P030908-70;
SOURCE 8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS
KEYWDS CS DOMAIN, BETA SANDWICH, STRUCTURAL GENOMICS, NPPSFA, NATIONAL
KEYWDS 2 PROJECT ON PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN
KEYWDS 3 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI, TRANSPORT PROTEIN
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR T.NAGASHIMA,F.HAYASHI,S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS
AUTHOR 2 INITIATIVE (RSGI)
REVDAT 3 09-MAR-22 2CR0 1 REMARK SEQADV
REVDAT 2 24-FEB-09 2CR0 1 VERSN
REVDAT 1 20-NOV-05 2CR0 0
JRNL AUTH T.NAGASHIMA,F.HAYASHI,S.YOKOYAMA
JRNL TITL SOLUTION STRUCTURE OF NUCLEAR MOVE DOMAIN OF NUCLEAR
JRNL TITL 2 DISTRIBUTION GENE C
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : VNMR 6.1C, CYANA 2.0.17
REMARK 3 AUTHORS : VARIAN (VNMR), GUNTERT, P. (CYANA)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2CR0 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 27-MAY-05.
REMARK 100 THE DEPOSITION ID IS D_1000024527.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 7.0
REMARK 210 IONIC STRENGTH : 120MM
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1.51MM PROTEIN U-13C,15N; 20MM
REMARK 210 TRISHCL, 100MM NACL, 1MM DTT,
REMARK 210 0.02% NAN3
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C
REMARK 210 -SEPARATED_NOESY
REMARK 210 SPECTROMETER FIELD STRENGTH : 800 MHZ
REMARK 210 SPECTROMETER MODEL : INOVA
REMARK 210 SPECTROMETER MANUFACTURER : VARIAN
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NMRPIPE 20020425, NMRVIEW 5.0.4,
REMARK 210 KUJIRA 0.901, CYANA 2.0.17
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : TARGET FUNCTION,STRUCTURES WITH
REMARK 210 THE LOWEST ENERGY
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 ASN A 13 31.26 36.90
REMARK 500 1 ALA A 27 -69.16 -94.16
REMARK 500 1 LYS A 75 91.61 -43.91
REMARK 500 1 SER A 79 154.23 -45.39
REMARK 500 1 ASP A 85 27.05 48.14
REMARK 500 1 ILE A 96 -30.96 -37.11
REMARK 500 1 ASN A 113 77.91 -59.65
REMARK 500 2 ASN A 13 38.02 36.38
REMARK 500 2 ARG A 36 70.63 -69.07
REMARK 500 2 LYS A 60 136.38 -39.90
REMARK 500 2 LYS A 75 92.26 -43.74
REMARK 500 2 SER A 79 156.85 -44.88
REMARK 500 2 LYS A 87 -43.25 -131.43
REMARK 500 3 ASN A 10 -75.09 -112.40
REMARK 500 3 ASN A 13 32.77 34.46
REMARK 500 3 LEU A 26 -28.44 -37.19
REMARK 500 3 ARG A 36 80.30 -63.46
REMARK 500 3 LYS A 75 93.10 -46.04
REMARK 500 3 LYS A 87 -31.94 -134.36
REMARK 500 3 MET A 99 32.29 -93.02
REMARK 500 3 THR A 107 -39.57 -33.35
REMARK 500 3 PRO A 118 98.69 -69.81
REMARK 500 4 SER A 3 42.06 -85.61
REMARK 500 4 ASN A 13 33.33 35.28
REMARK 500 4 ARG A 36 49.48 -79.85
REMARK 500 4 LYS A 75 95.68 -43.11
REMARK 500 4 SER A 79 152.90 -47.04
REMARK 500 4 SER A 80 147.30 -170.40
REMARK 500 5 LEU A 26 -28.44 -37.30
REMARK 500 5 ARG A 36 78.58 -60.84
REMARK 500 5 LYS A 60 -72.37 -31.61
REMARK 500 5 LYS A 75 90.74 -49.23
REMARK 500 5 ARG A 104 138.98 -171.73
REMARK 500 5 ASN A 113 96.10 -49.53
REMARK 500 6 SER A 5 -50.01 -125.73
REMARK 500 6 LEU A 11 48.47 -76.54
REMARK 500 6 LEU A 26 -29.86 -36.16
REMARK 500 6 ARG A 36 78.90 -62.84
REMARK 500 6 LYS A 75 95.86 -43.51
REMARK 500 6 SER A 79 153.33 -49.71
REMARK 500 6 MET A 99 32.58 -95.98
REMARK 500 6 PRO A 118 3.14 -69.80
REMARK 500 6 SER A 119 134.61 -33.50
REMARK 500 6 SER A 120 124.61 -36.66
REMARK 500 7 PRO A 9 97.99 -69.69
REMARK 500 7 ASN A 10 -174.88 -65.57
REMARK 500 7 LEU A 11 43.61 -79.55
REMARK 500 7 ALA A 27 -47.56 -133.11
REMARK 500 7 ARG A 36 46.95 -92.61
REMARK 500 7 LYS A 75 95.54 -45.20
REMARK 500
REMARK 500 THIS ENTRY HAS 152 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: MMT007103799.2 RELATED DB: TARGETDB
DBREF 2CR0 A 8 115 UNP O35685 NUDC_MOUSE 161 268
SEQADV 2CR0 GLY A 1 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 2 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 3 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 GLY A 4 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 5 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 6 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 GLY A 7 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 116 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 GLY A 117 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 PRO A 118 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 119 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 SER A 120 UNP O35685 CLONING ARTIFACT
SEQADV 2CR0 GLY A 121 UNP O35685 CLONING ARTIFACT
SEQRES 1 A 121 GLY SER SER GLY SER SER GLY LYS PRO ASN LEU GLY ASN
SEQRES 2 A 121 GLY ALA ASP LEU PRO ASN TYR ARG TRP THR GLN THR LEU
SEQRES 3 A 121 ALA GLU LEU ASP LEU ALA VAL PRO PHE ARG VAL SER PHE
SEQRES 4 A 121 ARG LEU LYS GLY LYS ASP VAL VAL VAL ASP ILE GLN ARG
SEQRES 5 A 121 ARG HIS LEU ARG VAL GLY LEU LYS GLY GLN PRO PRO VAL
SEQRES 6 A 121 VAL ASP GLY GLU LEU TYR ASN GLU VAL LYS VAL GLU GLU
SEQRES 7 A 121 SER SER TRP LEU ILE GLU ASP GLY LYS VAL VAL THR VAL
SEQRES 8 A 121 HIS LEU GLU LYS ILE ASN LYS MET GLU TRP TRP ASN ARG
SEQRES 9 A 121 LEU VAL THR SER ASP PRO GLU ILE ASN THR LYS SER GLY
SEQRES 10 A 121 PRO SER SER GLY
SHEET 1 A 5 ALA A 15 ASP A 16 0
SHEET 2 A 5 ARG A 21 GLN A 24 -1 O TRP A 22 N ALA A 15
SHEET 3 A 5 GLU A 28 PRO A 34 -1 O ASP A 30 N THR A 23
SHEET 4 A 5 VAL A 88 GLU A 94 -1 O LEU A 93 N LEU A 29
SHEET 5 A 5 SER A 80 GLU A 84 -1 N SER A 80 O HIS A 92
SHEET 1 B 3 ASP A 49 ILE A 50 0
SHEET 2 B 3 HIS A 54 VAL A 57 -1 O ARG A 56 N ASP A 49
SHEET 3 B 3 VAL A 66 GLU A 69 -1 O VAL A 66 N VAL A 57
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
