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Entry: 2DCP
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HEADER    UNKNOWN FUNCTION                        12-JAN-06   2DCP              
TITLE     FULLY AUTOMATED NMR STRUCTURE DETERMINATION OF THE ENTH-VHS           
TITLE    2 DOMAIN AT3G16270 FROM ARABIDOPSIS THALIANA                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: HYPOTHETICAL PROTEIN (RAFL09-17-B18);                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: ENTH-VHS DOMAIN AT3G16270;                                 
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ARABIDOPSIS THALIANA;                           
SOURCE   3 ORGANISM_COMMON: THALE CRESS;                                        
SOURCE   4 ORGANISM_TAXID: 3702;                                                
SOURCE   5 GENE: RIKEN CDNA RAFL09-17-B18;                                      
SOURCE   6 EXPRESSION_SYSTEM: CELL FREE SYNTHESIS;                              
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: P021216-68;                               
SOURCE   9 OTHER_DETAILS: E.COLI CELL-FREE PROTEIN SYNTHESIS                    
KEYWDS    FULLY AUTOMATED STRUCTURE DETERMINATION; FLYA ALGORITHM,              
KEYWDS   2 UNKNOWN FUNCTION                                                     
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    B.LOPEZ-MENDEZ,P.GUNTERT                                              
REVDAT   2   24-FEB-09 2DCP    1       VERSN                                    
REVDAT   1   17-OCT-06 2DCP    0                                                
JRNL        AUTH   B.LOPEZ-MENDEZ,P.GUNTERT                                     
JRNL        TITL   AUTOMATED PROTEIN STRUCTURE DETERMINATION FROM NMR           
JRNL        TITL 2 SPECTRA                                                      
JRNL        REF    J.AM.CHEM.SOC.                V. 128 13112 2006              
JRNL        REFN                   ISSN 0002-7863                               
JRNL        PMID   17017791                                                     
JRNL        DOI    10.1021/JA061136L                                            
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   B.LOPEZ-MENDEZ,D.PANTOJA-UCEDA,T.TOMIZAWA,                   
REMARK   1  AUTH 2 S.KOSHIBA,T.KIGAWA,M.SHIROUZU,T.TERADA,M.INOUE,              
REMARK   1  AUTH 3 T.YABUKI,M.AOKI,E.SEKI,T.MATSUDA,H.HIROTA,                   
REMARK   1  AUTH 4 M.YOSHIDA,A.TANAKA,T.OSANAI,M.SEKI,K.SHINOZAKI,              
REMARK   1  AUTH 5 S.YOKOYAMA,P.GUNTERT                                         
REMARK   1  TITL   NMR ASSIGNMENT OF THE HYPOTHETICAL ENTH-VHS DOMAIN           
REMARK   1  TITL 2 AT3G16270 FROM ARABIDOPSIS THALIANA                          
REMARK   1  REF    J.BIOMOL.NMR                  V.  29   205 2004              
REMARK   1  REFN                   ISSN 0925-2738                               
REMARK   1  PMID   15014234                                                     
REMARK   1  DOI    10.1023/B:JNMR.0000019239.44783.66                           
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : OPALP 1.2                                            
REMARK   3   AUTHORS     : KORADI, R., BILLETER, M., GUNTERT, P.                
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2DCP COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 13-JAN-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB025258.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.5                                
REMARK 210  IONIC STRENGTH                 : 100MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.20MM 13C, 15N-ARABIDOPSIS        
REMARK 210                                   ENTH-VHS DOMAIN; 20MM TRIS         
REMARK 210                                   BUFFER; 100MM NACL; 1MM            
REMARK 210                                   DITHIOTHREITOL; 0.02% NA3N;        
REMARK 210                                   90% H2O, 10% D2O                   
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : DRX, AVANCE                        
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : FLYA 1.0, CYANA 2.1                
REMARK 210   METHOD USED                   : FULLY AUTOMATED NMR SPECTRUM       
REMARK 210                                   ANALYSIS AND STRUCTURE             
REMARK 210                                   CALCULATION WITHOUT HUMAN          
REMARK 210                                   INTERVENTION. CHEMICAL SHIFT       
REMARK 210                                   ASSIGNMENTS AND CONFORMATIONAL     
REMARK 210                                   RESTRAINTS HAVE NOT BEEN           
REMARK 210                                   VERIFIED MANUALLY.                 
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  2 ARG A  14   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.1 DEGREES          
REMARK 500 11 ARG A  90   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.1 DEGREES          
REMARK 500 12 ARG A  14   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.0 DEGREES          
REMARK 500 20 ARG A  71   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.6 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   2      -81.01    -72.72                                   
REMARK 500  1 SER A   6       96.08    -47.33                                   
REMARK 500  1 LYS A  79      -46.17   -141.28                                   
REMARK 500  1 SER A  80      -78.40    -59.27                                   
REMARK 500  1 LYS A 102      -57.40   -136.63                                   
REMARK 500  2 LYS A  79      -44.89   -142.19                                   
REMARK 500  2 LYS A 102      -43.88   -141.03                                   
REMARK 500  2 PRO A 105     -174.21    -65.33                                   
REMARK 500  2 LEU A 108      -39.18   -140.61                                   
REMARK 500  2 GLU A 131     -175.75    -67.96                                   
REMARK 500  2 SER A 135       85.64    -69.38                                   
REMARK 500  3 SER A   5       78.88   -159.79                                   
REMARK 500  3 GLU A   8       64.26     28.07                                   
REMARK 500  3 ASP A  22       72.64    -60.76                                   
REMARK 500  3 LYS A  79      -50.40   -130.02                                   
REMARK 500  3 SER A  80       24.71    -63.31                                   
REMARK 500  3 ASP A 111       15.73     59.10                                   
REMARK 500  3 SER A 135       -6.16     55.53                                   
REMARK 500  4 SER A   2       82.68     26.32                                   
REMARK 500  4 SER A   6      -70.13    -91.88                                   
REMARK 500  4 GLU A   8      108.93    -47.37                                   
REMARK 500  4 ASP A  22       83.68    -63.10                                   
REMARK 500  4 LYS A  61       69.26   -104.08                                   
REMARK 500  4 LYS A  79      -43.05   -146.08                                   
REMARK 500  4 LYS A 102      -20.37   -151.14                                   
REMARK 500  4 LEU A 108      -23.49   -145.98                                   
REMARK 500  4 ASP A 111        3.15     57.68                                   
REMARK 500  5 SER A   3     -179.29    -67.66                                   
REMARK 500  5 ILE A  73      -74.25    -67.35                                   
REMARK 500  5 LYS A  79      -67.50   -141.11                                   
REMARK 500  5 SER A  80      -72.53    -43.00                                   
REMARK 500  5 ASN A  97        2.20    -66.08                                   
REMARK 500  5 LYS A 102      -51.70   -128.99                                   
REMARK 500  6 ASP A  59        0.20    -63.67                                   
REMARK 500  6 ASN A  60     -153.54    -65.97                                   
REMARK 500  6 ILE A  73      -71.81    -59.87                                   
REMARK 500  6 LYS A  79      -71.63    -92.16                                   
REMARK 500  6 LYS A 102      -50.27   -133.91                                   
REMARK 500  7 SER A   5      -24.67     70.86                                   
REMARK 500  7 SER A  21      107.35    -58.32                                   
REMARK 500  7 SER A  80       34.96    -87.29                                   
REMARK 500  7 LYS A 102      -36.51   -135.74                                   
REMARK 500  7 PRO A 105     -172.96    -69.08                                   
REMARK 500  7 LEU A 108      -47.69   -146.78                                   
REMARK 500  7 LYS A 109       -8.84    -59.76                                   
REMARK 500  7 ASP A 111       17.50     50.56                                   
REMARK 500  8 ASP A  22      100.63    -59.17                                   
REMARK 500  8 ILE A  73      -73.56    -63.42                                   
REMARK 500  8 LYS A  79      -45.15   -141.35                                   
REMARK 500  8 LYS A 102      -48.04   -146.68                                   
REMARK 500  8 ASN A 133      -16.46     55.69                                   
REMARK 500  8 SER A 135      -14.48     65.96                                   
REMARK 500  9 SER A  62      144.24   -178.48                                   
REMARK 500  9 ILE A  73      -72.35    -61.59                                   
REMARK 500  9 SER A  80       20.90    -65.91                                   
REMARK 500  9 LYS A 102      -45.25   -134.41                                   
REMARK 500  9 PRO A 105     -174.90    -66.60                                   
REMARK 500  9 SER A 130       14.40     56.11                                   
REMARK 500  9 GLU A 132       41.10    -73.64                                   
REMARK 500 10 SER A  41        1.39    -69.74                                   
REMARK 500 10 SER A  62      134.44   -171.15                                   
REMARK 500 10 ILE A  73      -74.00    -79.10                                   
REMARK 500 10 LYS A 102      -42.90   -134.13                                   
REMARK 500 10 GLU A 131     -168.69    -73.58                                   
REMARK 500 11 SER A   2     -179.52    -67.62                                   
REMARK 500 11 SER A  45       -8.95    -56.40                                   
REMARK 500 11 SER A  62      157.12    155.28                                   
REMARK 500 11 ILE A  73      -73.59    -65.62                                   
REMARK 500 11 LYS A  79      -57.10   -122.19                                   
REMARK 500 11 SER A  80      -70.61    -47.71                                   
REMARK 500 11 HIS A 104      152.35     71.28                                   
REMARK 500 11 LEU A 108      -35.78   -132.70                                   
REMARK 500 11 GLU A 132       51.46   -145.61                                   
REMARK 500 11 SER A 139      -17.16     67.18                                   
REMARK 500 12 ASP A  22       89.24    -63.65                                   
REMARK 500 12 SER A  62      142.24    173.14                                   
REMARK 500 12 ILE A  73      -71.51    -61.62                                   
REMARK 500 12 LYS A 102      -46.52   -150.30                                   
REMARK 500 12 GLU A 132      -65.62    -25.64                                   
REMARK 500 12 SER A 135        8.52   -152.72                                   
REMARK 500 13 ASP A  22       60.11    -69.66                                   
REMARK 500 13 SER A  41        7.58    -69.82                                   
REMARK 500 13 SER A  62      131.73    141.48                                   
REMARK 500 13 ILE A  73      -71.45    -63.46                                   
REMARK 500 13 LYS A 102      -34.96   -141.99                                   
REMARK 500 13 LEU A 108      -47.25   -141.59                                   
REMARK 500 13 SER A 130       73.17     26.66                                   
REMARK 500 13 SER A 135       17.24     46.29                                   
REMARK 500 14 SER A   5       17.36     53.70                                   
REMARK 500 14 ILE A  73      -72.32    -60.36                                   
REMARK 500 14 SER A  80        7.97    -60.62                                   
REMARK 500 14 SER A  82      -25.54    118.45                                   
REMARK 500 14 LEU A 108      -42.07   -136.53                                   
REMARK 500 14 GLU A 131     -139.40    -98.28                                   
REMARK 500 14 SER A 135       -7.90     58.87                                   
REMARK 500 15 SER A   2      -70.78    -65.62                                   
REMARK 500 15 SER A   5       76.15   -163.47                                   
REMARK 500 15 GLU A   8       96.73    -49.97                                   
REMARK 500 15 ASP A  22       92.11    -63.17                                   
REMARK 500 15 SER A  41       24.66    -79.44                                   
REMARK 500 15 ILE A  73      -74.39    -60.24                                   
REMARK 500 15 LYS A  79      -31.68   -139.17                                   
REMARK 500 15 SER A  80       36.85    -74.12                                   
REMARK 500 15 LYS A 102      -30.89   -155.77                                   
REMARK 500 15 LEU A 108      -23.15   -140.44                                   
REMARK 500 15 SER A 135     -163.73    -77.72                                   
REMARK 500 15 PRO A 137      -70.69    -69.66                                   
REMARK 500 15 SER A 139      -66.77   -142.36                                   
REMARK 500 16 SER A   3      -19.90   -143.99                                   
REMARK 500 16 ASP A  22       93.51    -67.38                                   
REMARK 500 16 SER A  62      140.95     98.73                                   
REMARK 500 16 LYS A  79      -48.35   -143.02                                   
REMARK 500 16 LYS A 102      -29.45   -147.33                                   
REMARK 500 16 ASN A 133       66.53   -110.12                                   
REMARK 500 16 SER A 138       12.07     54.96                                   
REMARK 500 17 SER A  62      148.40   -171.83                                   
REMARK 500 17 LYS A 102      -52.56   -137.10                                   
REMARK 500 17 LEU A 108      -27.79   -141.87                                   
REMARK 500 18 ASP A  22       84.83     12.13                                   
REMARK 500 18 LYS A  61       72.67   -115.33                                   
REMARK 500 18 ILE A  73      -75.76    -66.79                                   
REMARK 500 18 LYS A  79      -36.75   -134.51                                   
REMARK 500 18 LYS A 102      -45.23   -151.55                                   
REMARK 500 18 LEU A 108      -49.12   -141.30                                   
REMARK 500 18 SER A 130       99.91    -58.74                                   
REMARK 500 18 GLU A 131       82.93     39.55                                   
REMARK 500 18 GLU A 132      174.55    -55.64                                   
REMARK 500 18 SER A 135       15.19   -148.70                                   
REMARK 500 19 SER A   5      175.95    175.85                                   
REMARK 500 19 ASP A  24      -30.50     53.66                                   
REMARK 500 19 LYS A 102      -42.49   -141.29                                   
REMARK 500 19 GLU A 132       36.75    -71.68                                   
REMARK 500 19 SER A 138       18.67     55.65                                   
REMARK 500 20 ARG A  71       11.43    -69.10                                   
REMARK 500 20 ILE A  73      -75.18    -77.52                                   
REMARK 500 20 LYS A  79      -60.69   -139.63                                   
REMARK 500 20 GLU A  83      -76.85    -87.42                                   
REMARK 500 20 LYS A 102      -52.25   -144.23                                   
REMARK 500 20 GLU A 119      -60.63   -123.38                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 GLY A    4     SER A    5          6       149.80                    
REMARK 500 SER A  139     GLY A  140         11       133.80                    
REMARK 500 GLY A    1     SER A    2         13       147.58                    
REMARK 500 SER A  139     GLY A  140         13       142.26                    
REMARK 500 SER A  139     GLY A  140         16       149.94                    
REMARK 500 ASN A  133     GLY A  134         20       146.69                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  2 TYR A 101         0.08    SIDE_CHAIN                              
REMARK 500  3 ARG A  71         0.09    SIDE_CHAIN                              
REMARK 500  3 ARG A 118         0.13    SIDE_CHAIN                              
REMARK 500  4 ARG A  71         0.08    SIDE_CHAIN                              
REMARK 500  5 ARG A 118         0.10    SIDE_CHAIN                              
REMARK 500  6 ARG A  57         0.10    SIDE_CHAIN                              
REMARK 500  6 ARG A 118         0.08    SIDE_CHAIN                              
REMARK 500  7 ARG A  14         0.09    SIDE_CHAIN                              
REMARK 500  7 ARG A 118         0.09    SIDE_CHAIN                              
REMARK 500  8 ARG A  12         0.10    SIDE_CHAIN                              
REMARK 500  8 PHE A  50         0.08    SIDE_CHAIN                              
REMARK 500  9 PHE A  50         0.09    SIDE_CHAIN                              
REMARK 500 10 ARG A  40         0.10    SIDE_CHAIN                              
REMARK 500 11 ARG A  90         0.08    SIDE_CHAIN                              
REMARK 500 12 ARG A  40         0.08    SIDE_CHAIN                              
REMARK 500 12 ARG A  96         0.09    SIDE_CHAIN                              
REMARK 500 13 ARG A  57         0.10    SIDE_CHAIN                              
REMARK 500 13 ARG A  96         0.09    SIDE_CHAIN                              
REMARK 500 14 PHE A  50         0.10    SIDE_CHAIN                              
REMARK 500 14 ARG A  85         0.14    SIDE_CHAIN                              
REMARK 500 15 TYR A  10         0.07    SIDE_CHAIN                              
REMARK 500 15 ARG A  14         0.09    SIDE_CHAIN                              
REMARK 500 15 ARG A  71         0.08    SIDE_CHAIN                              
REMARK 500 15 ARG A  96         0.08    SIDE_CHAIN                              
REMARK 500 15 ARG A 118         0.12    SIDE_CHAIN                              
REMARK 500 16 ARG A  12         0.10    SIDE_CHAIN                              
REMARK 500 16 PHE A  50         0.08    SIDE_CHAIN                              
REMARK 500 17 PHE A  50         0.08    SIDE_CHAIN                              
REMARK 500 18 ARG A  40         0.18    SIDE_CHAIN                              
REMARK 500 18 PHE A  50         0.09    SIDE_CHAIN                              
REMARK 500 18 TYR A 101         0.10    SIDE_CHAIN                              
REMARK 500 19 PHE A  50         0.08    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1VDY   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF THE SAME PROTEIN DETERMINED BY CONVENTIONAL             
REMARK 900 NMR METHODS                                                          
DBREF  2DCP A    8   134  UNP    Q9C5H4   Q9C5H4_ARATH     9    135             
SEQADV 2DCP GLY A    1  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A    2  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A    3  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP GLY A    4  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A    5  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A    6  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP GLY A    7  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A  135  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP GLY A  136  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP PRO A  137  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A  138  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP SER A  139  UNP  Q9C5H4              CLONING ARTIFACT               
SEQADV 2DCP GLY A  140  UNP  Q9C5H4              CLONING ARTIFACT               
SEQRES   1 A  140  GLY SER SER GLY SER SER GLY GLU SER TYR TRP ARG SER          
SEQRES   2 A  140  ARG MET ILE ASP ALA VAL THR SER ASP GLU ASP LYS VAL          
SEQRES   3 A  140  ALA PRO VAL TYR LYS LEU GLU GLU ILE CYS ASP LEU LEU          
SEQRES   4 A  140  ARG SER SER HIS VAL SER ILE VAL LYS GLU PHE SER GLU          
SEQRES   5 A  140  PHE ILE LEU LYS ARG LEU ASP ASN LYS SER PRO ILE VAL          
SEQRES   6 A  140  LYS GLN LYS ALA LEU ARG LEU ILE LYS TYR ALA VAL GLY          
SEQRES   7 A  140  LYS SER GLY SER GLU PHE ARG ARG GLU MET GLN ARG ASN          
SEQRES   8 A  140  SER VAL ALA VAL ARG ASN LEU PHE HIS TYR LYS GLY HIS          
SEQRES   9 A  140  PRO ASP PRO LEU LYS GLY ASP ALA LEU ASN LYS ALA VAL          
SEQRES  10 A  140  ARG GLU THR ALA HIS GLU THR ILE SER ALA ILE PHE SER          
SEQRES  11 A  140  GLU GLU ASN GLY SER GLY PRO SER SER GLY                      
HELIX    1   1 TYR A   10  VAL A   19  1                                  10    
HELIX    2   2 PRO A   28  SER A   42  1                                  15    
HELIX    3   3 HIS A   43  LYS A   56  1                                  14    
HELIX    4   4 SER A   62  GLY A   78  1                                  17    
HELIX    5   5 GLY A   81  ARG A   90  1                                  10    
HELIX    6   6 SER A   92  ASN A   97  1                                   6    
HELIX    7   7 ASP A  111  PHE A  129  1                                  19    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system