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Database: PDB
Entry: 2E8P
LinkDB: 2E8P
Original site: 2E8P 
HEADER    SIGNALING PROTEIN                       22-JAN-07   2E8P              
TITLE     SOLUTION STRUCTURE OF THE N-TERMINAL SAM-DOMAIN OF E74-LIKE           
TITLE    2 FACTOR 3                                                             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ELF3 PROTEIN;                                              
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: SAM DOMAIN;                                                
COMPND   5 SYNONYM: E74-LIKE FACTOR 3;                                          
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ELF3;                                                          
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P060313-01;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    CELL-FREE PROTEIN SYNTHESIS, PROTEIN REGULATION, STRUCTURAL           
KEYWDS   2 GENOMICS, NPPSFA, NATIONAL PROJECT ON PROTEIN STRUCTURAL             
KEYWDS   3 AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL                            
KEYWDS   4 GENOMICS/PROTEOMICS INITIATIVE, RSGI, SIGNALING PROTEIN              
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    A.K.GORONCY,M.SATO,S.KOSHIBA,S.WATANABE,T.HARADA,T.KIGAWA,            
AUTHOR   2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE           
AUTHOR   3 (RSGI)                                                               
REVDAT   2   24-FEB-09 2E8P    1       VERSN                                    
REVDAT   1   24-JUL-07 2E8P    0                                                
JRNL        AUTH   A.K.GORONCY,M.SATO,S.KOSHIBA,S.WATANABE,T.HARADA,            
JRNL        AUTH 2 T.KIGAWA,S.YOKOYAMA                                          
JRNL        TITL   SOLUTION STRUCTURE OF THE N-TERMINAL SAM-DOMAIN OF           
JRNL        TITL 2 E74-LIKE FACTOR 3                                            
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.2.2                                          
REMARK   3   AUTHORS     : P.GUNTERT ET AL.                                     
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2E8P COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 25-JAN-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB026365.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 296                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.18MM SAM DOMAIN, 20MM D-         
REMARK 210                                   TRIS-HCL, 100MM NACL, 1MM D-       
REMARK 210                                   DTT, 0.02% NAN3; 90% H2O, 10%      
REMARK 210                                   D2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : CYANA 2.2.2, XWINNMR 3.5,          
REMARK 210                                   NMRPIPE 20060524, NMRVIEW          
REMARK 210                                   5.0.4, KUJIRA 0.899A               
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   3       70.97     52.64                                   
REMARK 500  1 SER A   6      142.31   -170.43                                   
REMARK 500  1 GLN A   8       55.64   -117.93                                   
REMARK 500  1 MET A   9      112.24   -178.66                                   
REMARK 500  1 SER A  10       91.89     57.27                                   
REMARK 500  1 GLU A  15       35.15   -142.21                                   
REMARK 500  1 LYS A  16     -178.08     61.24                                   
REMARK 500  1 ALA A  49      -42.29   -137.02                                   
REMARK 500  1 SER A  91     -172.38     60.36                                   
REMARK 500  2 SER A   6      -46.10   -167.51                                   
REMARK 500  2 SER A  10       59.67   -148.96                                   
REMARK 500  2 ALA A  49      -40.41   -133.81                                   
REMARK 500  2 SER A  91       80.59     54.16                                   
REMARK 500  3 SER A   6      174.81     61.81                                   
REMARK 500  3 LEU A  11       50.65   -147.34                                   
REMARK 500  3 GLU A  12      -69.68    -96.65                                   
REMARK 500  3 LYS A  16     -172.90     62.09                                   
REMARK 500  3 ALA A  49      -40.89   -135.79                                   
REMARK 500  3 ASP A  56       68.24   -108.93                                   
REMARK 500  3 CYS A  65      179.74    -58.08                                   
REMARK 500  3 SER A  90      103.45    -53.08                                   
REMARK 500  3 SER A  91      178.04     59.46                                   
REMARK 500  4 GLN A   8      141.58   -171.09                                   
REMARK 500  4 MET A   9       67.11   -173.07                                   
REMARK 500  4 LYS A  16      178.90     61.18                                   
REMARK 500  4 ALA A  49      -40.60   -134.06                                   
REMARK 500  4 ASP A  51       76.78   -107.99                                   
REMARK 500  4 CYS A  55       58.95    -99.14                                   
REMARK 500  5 SER A   6      -63.65    -99.36                                   
REMARK 500  5 GLN A   8       91.60     60.10                                   
REMARK 500  5 SER A  10     -179.99     59.20                                   
REMARK 500  5 ALA A  49      -41.42   -137.74                                   
REMARK 500  5 ASP A  56       68.41   -107.24                                   
REMARK 500  5 THR A  89      -72.32    -72.88                                   
REMARK 500  5 SER A  91     -177.25     59.42                                   
REMARK 500  6 SER A   6      145.38   -172.39                                   
REMARK 500  6 MET A   9     -170.95     53.53                                   
REMARK 500  6 SER A  10      178.04     58.30                                   
REMARK 500  6 LEU A  11       59.73   -165.98                                   
REMARK 500  6 GLU A  12       89.31   -160.90                                   
REMARK 500  6 GLU A  15       28.57   -147.06                                   
REMARK 500  6 ALA A  49      -41.45   -137.18                                   
REMARK 500  6 SER A  91       44.11   -102.80                                   
REMARK 500  7 SER A   5       74.54   -161.45                                   
REMARK 500  7 SER A   6     -177.37     57.55                                   
REMARK 500  7 SER A  10       30.06   -155.47                                   
REMARK 500  7 ALA A  49      -42.07   -145.29                                   
REMARK 500  8 SER A   6       39.37   -152.43                                   
REMARK 500  8 MET A   9       38.11   -171.81                                   
REMARK 500  8 GLU A  12      106.48    -57.02                                   
REMARK 500  8 CYS A  55       48.11    -99.16                                   
REMARK 500  8 SER A  91       49.26   -168.28                                   
REMARK 500  9 SER A   2      -63.82   -103.86                                   
REMARK 500  9 SER A   6      180.00     57.76                                   
REMARK 500  9 LYS A  16     -170.99     62.69                                   
REMARK 500  9 ALA A  49      -41.97   -138.91                                   
REMARK 500  9 SER A  91      136.83   -170.83                                   
REMARK 500 10 MET A   9       97.99     61.87                                   
REMARK 500 10 THR A  14      143.24   -176.49                                   
REMARK 500 10 ALA A  17      137.58   -171.94                                   
REMARK 500 10 ALA A  49      -39.91   -132.25                                   
REMARK 500 10 ASP A  56       68.30   -100.83                                   
REMARK 500 10 SER A  91     -176.18     55.97                                   
REMARK 500 11 SER A   5       88.23     55.83                                   
REMARK 500 11 SER A   6       42.16   -108.79                                   
REMARK 500 11 SER A  10      -52.99   -144.82                                   
REMARK 500 11 LEU A  11       88.77     62.32                                   
REMARK 500 11 GLU A  15      112.65     64.88                                   
REMARK 500 11 LYS A  16     -170.93    -66.33                                   
REMARK 500 11 ALA A  17      137.38   -171.85                                   
REMARK 500 11 ALA A  49      -40.57   -134.31                                   
REMARK 500 11 ILE A  50      106.83    -51.58                                   
REMARK 500 11 ASP A  51       86.46    -68.80                                   
REMARK 500 11 ASP A  56       68.40   -107.57                                   
REMARK 500 11 MET A  57     -169.93   -129.84                                   
REMARK 500 11 ASP A  58      -57.55   -127.80                                   
REMARK 500 11 SER A  90       71.33     60.17                                   
REMARK 500 12 SER A   5      -53.11   -130.96                                   
REMARK 500 12 SER A  10       96.99     61.98                                   
REMARK 500 12 GLU A  12      178.80     62.91                                   
REMARK 500 12 LYS A  16      178.83     62.12                                   
REMARK 500 12 ALA A  49      -40.66   -134.31                                   
REMARK 500 12 CYS A  55       86.56    -68.67                                   
REMARK 500 12 ASP A  58      -48.54   -137.01                                   
REMARK 500 12 SER A  90       75.74     54.13                                   
REMARK 500 13 SER A   3      142.14   -172.37                                   
REMARK 500 13 SER A   5       93.23     62.74                                   
REMARK 500 13 SER A   6       45.67   -169.02                                   
REMARK 500 13 GLN A   8      101.00     61.30                                   
REMARK 500 13 MET A   9      -55.09   -153.42                                   
REMARK 500 13 SER A  10      172.62    -55.24                                   
REMARK 500 13 ALA A  49      -41.48   -137.81                                   
REMARK 500 13 PHE A  52       46.50    -91.29                                   
REMARK 500 13 SER A  53      -42.11   -138.66                                   
REMARK 500 14 SER A   6       57.73   -113.41                                   
REMARK 500 14 GLN A   8     -173.27     60.08                                   
REMARK 500 14 LEU A  11       60.78   -111.70                                   
REMARK 500 14 LYS A  16     -176.95     57.16                                   
REMARK 500 14 SER A  90       95.42     58.87                                   
REMARK 500 15 SER A   2      177.76     62.32                                   
REMARK 500 15 SER A   5       27.34   -154.39                                   
REMARK 500 15 GLN A   8      118.62   -160.09                                   
REMARK 500 15 MET A   9       31.51   -150.17                                   
REMARK 500 15 LYS A  16     -176.09     63.19                                   
REMARK 500 15 ALA A  49      -40.98   -134.93                                   
REMARK 500 15 MET A  57     -174.14    -69.76                                   
REMARK 500 15 ASP A  58      -52.25   -126.58                                   
REMARK 500 15 SER A  91     -177.70     56.57                                   
REMARK 500 16 SER A   2      -71.31   -135.92                                   
REMARK 500 16 GLN A   8       82.48     54.98                                   
REMARK 500 16 LEU A  11      173.77     59.95                                   
REMARK 500 16 LYS A  16     -175.17     57.38                                   
REMARK 500 16 ALA A  49      -41.43   -137.50                                   
REMARK 500 16 PHE A  52       45.69    -91.78                                   
REMARK 500 16 SER A  53      -42.04   -138.56                                   
REMARK 500 16 SER A  91      100.54     61.15                                   
REMARK 500 17 SER A   3      -72.75   -173.61                                   
REMARK 500 17 SER A   5       65.30     61.36                                   
REMARK 500 17 MET A   9       43.92   -147.65                                   
REMARK 500 17 LYS A  16     -179.90     60.50                                   
REMARK 500 17 ALA A  49      -40.86   -134.64                                   
REMARK 500 17 CYS A  55       84.83    -69.80                                   
REMARK 500 18 LYS A  16      177.92     59.03                                   
REMARK 500 19 SER A   2      -42.51   -154.55                                   
REMARK 500 19 LYS A  16     -177.85     57.44                                   
REMARK 500 20 SER A   2     -172.89     54.74                                   
REMARK 500 20 GLU A  12      -65.84   -129.88                                   
REMARK 500 20 ALA A  17      136.15   -175.62                                   
REMARK 500 20 SER A  91      166.44     62.75                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSS001002151.1   RELATED DB: TARGETDB                    
DBREF  2E8P A    8    92  UNP    Q6IAP8   Q6IAP8_HUMAN    48    132             
SEQADV 2E8P GLY A    1  UNP  Q6IAP8              CLONING ARTIFACT               
SEQADV 2E8P SER A    2  UNP  Q6IAP8              CLONING ARTIFACT               
SEQADV 2E8P SER A    3  UNP  Q6IAP8              CLONING ARTIFACT               
SEQADV 2E8P GLY A    4  UNP  Q6IAP8              CLONING ARTIFACT               
SEQADV 2E8P SER A    5  UNP  Q6IAP8              CLONING ARTIFACT               
SEQADV 2E8P SER A    6  UNP  Q6IAP8              CLONING ARTIFACT               
SEQADV 2E8P GLY A    7  UNP  Q6IAP8              CLONING ARTIFACT               
SEQRES   1 A   92  GLY SER SER GLY SER SER GLY GLN MET SER LEU GLU GLY          
SEQRES   2 A   92  THR GLU LYS ALA SER TRP LEU GLY GLU GLN PRO GLN PHE          
SEQRES   3 A   92  TRP SER LYS THR GLN VAL LEU ASP TRP ILE SER TYR GLN          
SEQRES   4 A   92  VAL GLU LYS ASN LYS TYR ASP ALA SER ALA ILE ASP PHE          
SEQRES   5 A   92  SER ARG CYS ASP MET ASP GLY ALA THR LEU CYS ASN CYS          
SEQRES   6 A   92  ALA LEU GLU GLU LEU ARG LEU VAL PHE GLY PRO LEU GLY          
SEQRES   7 A   92  ASP GLN LEU HIS ALA GLN LEU ARG ASP LEU THR SER SER          
SEQRES   8 A   92  SER                                                          
HELIX    1   1 GLN A   23  TRP A   27  5                                   5    
HELIX    2   2 SER A   28  ASN A   43  1                                  16    
HELIX    3   3 GLY A   59  CYS A   65  1                                   7    
HELIX    4   4 ALA A   66  PHE A   74  1                                   9    
HELIX    5   5 LEU A   77  SER A   91  1                                  15    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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