HEADER SIGNALING PROTEIN 22-JAN-07 2E8P
TITLE SOLUTION STRUCTURE OF THE N-TERMINAL SAM-DOMAIN OF E74-LIKE FACTOR 3
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: ELF3 PROTEIN;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: SAM DOMAIN;
COMPND 5 SYNONYM: E74-LIKE FACTOR 3;
COMPND 6 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: ELF3;
SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: P060313-01;
SOURCE 8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS
KEYWDS CELL-FREE PROTEIN SYNTHESIS, PROTEIN REGULATION, STRUCTURAL GENOMICS,
KEYWDS 2 NPPSFA, NATIONAL PROJECT ON PROTEIN STRUCTURAL AND FUNCTIONAL
KEYWDS 3 ANALYSES, RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI,
KEYWDS 4 SIGNALING PROTEIN
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR A.K.GORONCY,M.SATO,S.KOSHIBA,S.WATANABE,T.HARADA,T.KIGAWA,S.YOKOYAMA,
AUTHOR 2 RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)
REVDAT 3 09-MAR-22 2E8P 1 REMARK SEQADV
REVDAT 2 24-FEB-09 2E8P 1 VERSN
REVDAT 1 24-JUL-07 2E8P 0
JRNL AUTH A.K.GORONCY,M.SATO,S.KOSHIBA,S.WATANABE,T.HARADA,T.KIGAWA,
JRNL AUTH 2 S.YOKOYAMA
JRNL TITL SOLUTION STRUCTURE OF THE N-TERMINAL SAM-DOMAIN OF E74-LIKE
JRNL TITL 2 FACTOR 3
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : CYANA 2.2.2
REMARK 3 AUTHORS : P.GUNTERT ET AL.
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2E8P COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 25-JAN-07.
REMARK 100 THE DEPOSITION ID IS D_1000026365.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 296
REMARK 210 PH : 7.0
REMARK 210 IONIC STRENGTH : 120MM
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1.18MM SAM DOMAIN, 20MM D-TRIS
REMARK 210 -HCL, 100MM NACL, 1MM D-DTT,
REMARK 210 0.02% NAN3; 90% H2O, 10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C
REMARK 210 -SEPARATED_NOESY
REMARK 210 SPECTROMETER FIELD STRENGTH : 800 MHZ
REMARK 210 SPECTROMETER MODEL : AVANCE
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : CYANA 2.2.2, XWINNMR 3.5,
REMARK 210 NMRPIPE 20060524, NMRVIEW 5.0.4,
REMARK 210 KUJIRA 0.899A
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LEAST
REMARK 210 RESTRAINT VIOLATIONS
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 SER A 3 70.97 52.64
REMARK 500 1 SER A 6 142.31 -170.43
REMARK 500 1 GLN A 8 55.64 -117.93
REMARK 500 1 MET A 9 112.24 -178.66
REMARK 500 1 SER A 10 91.89 57.27
REMARK 500 1 GLU A 15 35.15 -142.21
REMARK 500 1 LYS A 16 -178.08 61.24
REMARK 500 1 ALA A 49 -42.29 -137.02
REMARK 500 1 SER A 91 -172.38 60.36
REMARK 500 2 SER A 6 -46.10 -167.51
REMARK 500 2 SER A 10 59.67 -148.96
REMARK 500 2 ALA A 49 -40.41 -133.81
REMARK 500 2 SER A 91 80.59 54.16
REMARK 500 3 SER A 6 174.81 61.81
REMARK 500 3 LEU A 11 50.65 -147.34
REMARK 500 3 GLU A 12 -69.68 -96.65
REMARK 500 3 LYS A 16 -172.90 62.09
REMARK 500 3 ALA A 49 -40.89 -135.79
REMARK 500 3 ASP A 56 68.24 -108.93
REMARK 500 3 CYS A 65 179.74 -58.08
REMARK 500 3 SER A 90 103.45 -53.08
REMARK 500 3 SER A 91 178.04 59.46
REMARK 500 4 GLN A 8 141.58 -171.09
REMARK 500 4 MET A 9 67.11 -173.07
REMARK 500 4 LYS A 16 178.90 61.18
REMARK 500 4 ALA A 49 -40.60 -134.06
REMARK 500 4 ASP A 51 76.78 -107.99
REMARK 500 4 CYS A 55 58.95 -99.14
REMARK 500 5 SER A 6 -63.65 -99.36
REMARK 500 5 GLN A 8 91.60 60.10
REMARK 500 5 SER A 10 -179.99 59.20
REMARK 500 5 ALA A 49 -41.42 -137.74
REMARK 500 5 ASP A 56 68.41 -107.24
REMARK 500 5 THR A 89 -72.32 -72.88
REMARK 500 5 SER A 91 -177.25 59.42
REMARK 500 6 SER A 6 145.38 -172.39
REMARK 500 6 MET A 9 -170.95 53.53
REMARK 500 6 SER A 10 178.04 58.30
REMARK 500 6 LEU A 11 59.73 -165.98
REMARK 500 6 GLU A 12 89.31 -160.90
REMARK 500 6 GLU A 15 28.57 -147.06
REMARK 500 6 ALA A 49 -41.45 -137.18
REMARK 500 6 SER A 91 44.11 -102.80
REMARK 500 7 SER A 5 74.54 -161.45
REMARK 500 7 SER A 6 -177.37 57.55
REMARK 500 7 SER A 10 30.06 -155.47
REMARK 500 7 ALA A 49 -42.07 -145.29
REMARK 500 8 SER A 6 39.37 -152.43
REMARK 500 8 MET A 9 38.11 -171.81
REMARK 500 8 GLU A 12 106.48 -57.02
REMARK 500
REMARK 500 THIS ENTRY HAS 129 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: HSS001002151.1 RELATED DB: TARGETDB
DBREF 2E8P A 8 92 UNP Q6IAP8 Q6IAP8_HUMAN 48 132
SEQADV 2E8P GLY A 1 UNP Q6IAP8 CLONING ARTIFACT
SEQADV 2E8P SER A 2 UNP Q6IAP8 CLONING ARTIFACT
SEQADV 2E8P SER A 3 UNP Q6IAP8 CLONING ARTIFACT
SEQADV 2E8P GLY A 4 UNP Q6IAP8 CLONING ARTIFACT
SEQADV 2E8P SER A 5 UNP Q6IAP8 CLONING ARTIFACT
SEQADV 2E8P SER A 6 UNP Q6IAP8 CLONING ARTIFACT
SEQADV 2E8P GLY A 7 UNP Q6IAP8 CLONING ARTIFACT
SEQRES 1 A 92 GLY SER SER GLY SER SER GLY GLN MET SER LEU GLU GLY
SEQRES 2 A 92 THR GLU LYS ALA SER TRP LEU GLY GLU GLN PRO GLN PHE
SEQRES 3 A 92 TRP SER LYS THR GLN VAL LEU ASP TRP ILE SER TYR GLN
SEQRES 4 A 92 VAL GLU LYS ASN LYS TYR ASP ALA SER ALA ILE ASP PHE
SEQRES 5 A 92 SER ARG CYS ASP MET ASP GLY ALA THR LEU CYS ASN CYS
SEQRES 6 A 92 ALA LEU GLU GLU LEU ARG LEU VAL PHE GLY PRO LEU GLY
SEQRES 7 A 92 ASP GLN LEU HIS ALA GLN LEU ARG ASP LEU THR SER SER
SEQRES 8 A 92 SER
HELIX 1 1 GLN A 23 TRP A 27 5 5
HELIX 2 2 SER A 28 ASN A 43 1 16
HELIX 3 3 GLY A 59 CYS A 65 1 7
HELIX 4 4 ALA A 66 PHE A 74 1 9
HELIX 5 5 LEU A 77 SER A 91 1 15
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END