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Database: PDB
Entry: 2EDI
LinkDB: 2EDI
Original site: 2EDI 
HEADER    LIGASE                                  14-FEB-07   2EDI              
TITLE     SOLUTION STRUCTURE OF THE UQ_CON DOMAIN FROM HUMAN NEDD8-             
TITLE    2 CONJUGATING ENZYME NCE2                                              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: NEDD8-CONJUGATING ENZYME UBE2F;                            
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UQ_CON;                                                    
COMPND   5 SYNONYM: UBIQUITIN-CONJUGATING ENZYME E2 F, NEDD8 PROTEIN            
COMPND   6 LIGASE UBE2F, NEDD8 CARRIER PROTEIN UBE2F, NEDD8-                    
COMPND   7 CONJUGATING ENZYME 2, NEDD8-CONJUGATING ENZYME NCE2;                 
COMPND   8 EC: 6.3.2.-;                                                         
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: NCE2;                                                          
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P050314-14;                               
SOURCE   8 OTHER_DETAILS: CELL FREE PROTEIN SYNTHESIS                           
KEYWDS    NON-PROLINE CIS PEPTIDE BOND, E2 CONJUGATING ENZYME,                  
KEYWDS   2 STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT ON PROTEIN             
KEYWDS   3 STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL                 
KEYWDS   4 GENOMICS/PROTEOMICS INITIATIVE, RSGI, LIGASE                         
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    H.ENDO,K.IZUMI,M.YOSHIDA,F.HAYASHI,S.YOKOYAMA,RIKEN                   
AUTHOR   2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)                     
REVDAT   2   24-FEB-09 2EDI    1       VERSN                                    
REVDAT   1   14-AUG-07 2EDI    0                                                
JRNL        AUTH   H.ENDO,K.IZUMI,M.YOSHIDA,F.HAYASHI,S.YOKOYAMA                
JRNL        TITL   SOLUTION STRUCTURE OF THE UQ_CON DOMAIN FROM HUMAN           
JRNL        TITL 2 NEDD8-CONJUGATING ENZYME NCE2                                
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2EDI COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 20-FEB-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB026529.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 293                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.62MM 13C,15N-LABELED             
REMARK 210                                   PROTEIN; 20MM D-TRIS-              
REMARK 210                                   HCL(PH7.0); 100MM NACL; 1MM D-     
REMARK 210                                   DTT ; 0.02% NAN3; 10% D2O, 90%     
REMARK 210                                   H2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 900 MHZ, 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1C, NMRPIPE 20060702,       
REMARK 210                                   NMRVIEW 5.0.4, KUJIRA 0.9820,      
REMARK 210                                   CYANA 2.0.17                       
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   5       47.54     71.81                                   
REMARK 500  1 SER A   6      148.68   -172.56                                   
REMARK 500  1 ARG A  11      140.84    -34.82                                   
REMARK 500  1 SER A  13      -74.35   -133.48                                   
REMARK 500  1 VAL A  23      -39.35    -35.78                                   
REMARK 500  1 TYR A  58       37.12    -87.73                                   
REMARK 500  1 PRO A  70     -178.04    -69.75                                   
REMARK 500  1 TYR A  73      138.67    -31.10                                   
REMARK 500  1 THR A  92      171.58    -47.16                                   
REMARK 500  1 HIS A 105       40.62     34.85                                   
REMARK 500  1 SER A 106      102.03    -34.69                                   
REMARK 500  1 PRO A 114        1.27    -69.72                                   
REMARK 500  1 THR A 130      -64.31   -127.85                                   
REMARK 500  1 LEU A 132      -34.43    -34.02                                   
REMARK 500  1 LEU A 133       93.41    -35.05                                   
REMARK 500  1 ASP A 137       52.38   -119.87                                   
REMARK 500  1 TYR A 161      -19.27    -48.75                                   
REMARK 500  1 ILE A 162      -72.67    -82.26                                   
REMARK 500  2 PRO A  70     -164.93    -69.75                                   
REMARK 500  2 TYR A  73      138.13    -29.72                                   
REMARK 500  2 THR A 130      -61.17   -132.56                                   
REMARK 500  2 LEU A 132       46.68    -75.58                                   
REMARK 500  2 LEU A 133       74.98   -112.82                                   
REMARK 500  2 ASP A 136      -55.89   -124.31                                   
REMARK 500  2 PRO A 138     -173.08    -69.77                                   
REMARK 500  2 TYR A 161      -33.41    -36.71                                   
REMARK 500  3 LEU A  19      -63.81    -91.18                                   
REMARK 500  3 TYR A  58       33.66    -89.43                                   
REMARK 500  3 PRO A  70     -175.31    -69.75                                   
REMARK 500  3 TYR A  73      139.82    -32.08                                   
REMARK 500  3 TRP A  87       31.93    -87.08                                   
REMARK 500  3 THR A  92      172.05    -51.45                                   
REMARK 500  3 ARG A 103      138.24    -34.51                                   
REMARK 500  3 SER A 106      128.65   -170.28                                   
REMARK 500  3 PRO A 114        3.87    -69.73                                   
REMARK 500  3 THR A 130      -72.56   -129.90                                   
REMARK 500  3 LEU A 132      -33.39    -38.32                                   
REMARK 500  3 LEU A 133       94.32    -42.90                                   
REMARK 500  4 SER A   3       42.58     39.13                                   
REMARK 500  4 VAL A  23      -35.74    -36.14                                   
REMARK 500  4 LEU A  30      124.00    -36.92                                   
REMARK 500  4 PRO A  31     -175.15    -69.77                                   
REMARK 500  4 TYR A  58       37.42    -84.66                                   
REMARK 500  4 PRO A  70     -179.01    -69.75                                   
REMARK 500  4 TYR A  73      139.99    -32.29                                   
REMARK 500  4 TRP A  87       45.80    -83.27                                   
REMARK 500  4 SER A 106      136.16   -172.25                                   
REMARK 500  4 PRO A 114        2.45    -69.75                                   
REMARK 500  4 THR A 130      -70.15   -134.37                                   
REMARK 500  4 LEU A 132       41.92    -79.47                                   
REMARK 500  4 LEU A 133       66.77   -109.92                                   
REMARK 500  4 ASP A 137       55.30   -119.83                                   
REMARK 500  4 ALA A 143      -33.81    -34.78                                   
REMARK 500  4 ILE A 162      -71.06    -67.53                                   
REMARK 500  5 THR A   9      173.66    -48.51                                   
REMARK 500  5 VAL A  20      -37.92    -37.93                                   
REMARK 500  5 GLN A  59      -32.19    -39.21                                   
REMARK 500  5 PRO A  70     -175.60    -69.81                                   
REMARK 500  5 TYR A  73      138.09    -30.01                                   
REMARK 500  5 LEU A  83      -70.16    -56.31                                   
REMARK 500  5 THR A  84      161.37    -48.68                                   
REMARK 500  5 TRP A  87       31.86    -85.39                                   
REMARK 500  5 HIS A 105       69.06   -100.49                                   
REMARK 500  5 THR A 110      104.18    -55.67                                   
REMARK 500  5 THR A 130      -76.32   -108.04                                   
REMARK 500  5 LEU A 132       41.51    -80.73                                   
REMARK 500  5 LEU A 133       75.08   -116.16                                   
REMARK 500  5 ASP A 136      -65.40   -103.40                                   
REMARK 500  5 ASP A 137       53.11   -119.53                                   
REMARK 500  5 ILE A 162      -70.32    -62.28                                   
REMARK 500  6 SER A   6      131.25   -171.05                                   
REMARK 500  6 ARG A  11      164.19    -42.25                                   
REMARK 500  6 LEU A  19      -64.51    -90.71                                   
REMARK 500  6 LYS A  35      119.66   -162.71                                   
REMARK 500  6 TYR A  58       37.76    -91.98                                   
REMARK 500  6 GLN A  59      -33.27    -37.63                                   
REMARK 500  6 PRO A  70     -177.47    -69.76                                   
REMARK 500  6 TYR A  73      138.92    -31.68                                   
REMARK 500  6 TRP A  87       40.68    -86.15                                   
REMARK 500  6 THR A  92     -175.40    -53.22                                   
REMARK 500  6 PRO A 114        3.64    -69.80                                   
REMARK 500  6 THR A 130      -67.40   -131.78                                   
REMARK 500  6 LEU A 132      -31.79    -38.02                                   
REMARK 500  6 LEU A 133       91.71    -41.79                                   
REMARK 500  6 ASP A 136      -63.39   -107.53                                   
REMARK 500  6 ILE A 162      -71.84    -79.85                                   
REMARK 500  6 SER A 168      -54.34   -130.58                                   
REMARK 500  6 PRO A 170     -176.33    -69.78                                   
REMARK 500  7 SER A   8      147.90    -34.74                                   
REMARK 500  7 ASP A  16      -72.82    -51.13                                   
REMARK 500  7 PRO A  31     -178.65    -69.79                                   
REMARK 500  7 TYR A  58       39.30    -99.34                                   
REMARK 500  7 TYR A  73      139.05    -31.37                                   
REMARK 500  7 TRP A  87       35.69    -83.83                                   
REMARK 500  7 HIS A 105       44.53     34.74                                   
REMARK 500  7 THR A 130      -71.38   -135.03                                   
REMARK 500  7 LEU A 132       41.17    -83.93                                   
REMARK 500  7 PHE A 135       41.49    -98.28                                   
REMARK 500  7 ASP A 137       51.35   -118.00                                   
REMARK 500  7 PRO A 138     -173.05    -69.78                                   
REMARK 500  7 ASP A 150      110.41   -160.17                                   
REMARK 500  7 ILE A 162      -71.01    -67.13                                   
REMARK 500  8 SER A   3      118.60   -163.22                                   
REMARK 500  8 VAL A  12      -25.56    -39.20                                   
REMARK 500  8 LEU A  19      -60.31    -92.32                                   
REMARK 500  8 LYS A  35      114.79   -162.39                                   
REMARK 500  8 TYR A  58       37.06    -86.14                                   
REMARK 500  8 TYR A  73      140.24    -34.98                                   
REMARK 500  8 TRP A  87       42.42    -81.19                                   
REMARK 500  8 THR A  92      157.68    -46.29                                   
REMARK 500  8 CYS A  98       79.91   -103.61                                   
REMARK 500  8 THR A 110      -39.65    -38.24                                   
REMARK 500  8 PRO A 114        2.13    -69.79                                   
REMARK 500  8 LEU A 125      -33.13    -39.73                                   
REMARK 500  8 THR A 130      -76.79   -102.19                                   
REMARK 500  8 LEU A 132       41.85    -80.76                                   
REMARK 500  8 ASN A 134      111.70    -38.49                                   
REMARK 500  8 SER A 172      -50.42   -133.78                                   
REMARK 500  9 THR A   9      -33.02   -130.06                                   
REMARK 500  9 TYR A  58       34.68    -91.05                                   
REMARK 500  9 GLN A  59      -38.44    -39.63                                   
REMARK 500  9 TYR A  73      138.91    -31.64                                   
REMARK 500  9 TRP A  87       33.24    -83.46                                   
REMARK 500  9 HIS A 105       49.01     33.81                                   
REMARK 500  9 SER A 106       97.20    -55.36                                   
REMARK 500  9 THR A 130      -70.21   -113.81                                   
REMARK 500  9 LEU A 132      -32.27    -34.88                                   
REMARK 500  9 LEU A 133      102.88    -34.27                                   
REMARK 500  9 ASP A 136      -61.96   -104.35                                   
REMARK 500  9 TYR A 161      -31.37    -34.92                                   
REMARK 500 10 VAL A  12      -33.71    -36.73                                   
REMARK 500 10 LEU A  30      129.82    -39.79                                   
REMARK 500 10 TYR A  58       43.03    -84.82                                   
REMARK 500 10 PRO A  70     -173.33    -69.80                                   
REMARK 500 10 TYR A  73      139.85    -33.31                                   
REMARK 500 10 TRP A  87       42.64    -80.72                                   
REMARK 500 10 HIS A 105       44.84    -81.17                                   
REMARK 500 10 THR A 130      -78.58    -76.72                                   
REMARK 500 10 LEU A 132       38.87    -80.89                                   
REMARK 500 10 LEU A 133       75.78   -114.56                                   
REMARK 500 10 ASP A 136      -70.94   -106.03                                   
REMARK 500 10 ASP A 137       57.38   -116.39                                   
REMARK 500 10 PRO A 138     -175.94    -69.76                                   
REMARK 500 10 ILE A 162      -73.18    -69.43                                   
REMARK 500 10 PRO A 170        2.22    -69.77                                   
REMARK 500 10 SER A 171      169.41    -46.69                                   
REMARK 500 11 SER A  13      -72.56   -134.88                                   
REMARK 500 11 LYS A  35      115.86   -164.40                                   
REMARK 500 11 TYR A  58       33.81    -84.06                                   
REMARK 500 11 TYR A  73      140.19    -33.57                                   
REMARK 500 11 TRP A  87       45.98    -78.19                                   
REMARK 500 11 CYS A  98       69.76   -114.77                                   
REMARK 500 11 ILE A 107       40.59     28.66                                   
REMARK 500 11 ASP A 108      -45.21   -134.79                                   
REMARK 500 11 PRO A 114        2.54    -69.78                                   
REMARK 500 11 LEU A 128      -19.91    -47.12                                   
REMARK 500 11 THR A 130      -70.72   -132.66                                   
REMARK 500 11 PHE A 135       41.73    -96.38                                   
REMARK 500 11 ASP A 136      -62.53   -103.38                                   
REMARK 500 11 TYR A 161      -33.44    -35.03                                   
REMARK 500 11 ILE A 162      -73.69    -65.72                                   
REMARK 500 11 LYS A 163      -36.03    -37.24                                   
REMARK 500 12 SER A   6      109.09    -51.71                                   
REMARK 500 12 THR A   9      144.65    -34.56                                   
REMARK 500 12 ASP A  16      -68.69    -92.65                                   
REMARK 500 12 TYR A  58       34.95    -93.29                                   
REMARK 500 12 TYR A  73      138.36    -30.91                                   
REMARK 500 12 TRP A  87       43.48    -86.51                                   
REMARK 500 12 PRO A  89        2.43    -69.77                                   
REMARK 500 12 LEU A 102       34.19    -93.02                                   
REMARK 500 12 HIS A 105       40.02     34.88                                   
REMARK 500 12 SER A 106      156.47    -46.31                                   
REMARK 500 12 THR A 110      -75.24    -56.30                                   
REMARK 500 12 PRO A 114        0.90    -69.82                                   
REMARK 500 12 ASP A 131      -36.80    -30.95                                   
REMARK 500 12 LEU A 133      129.24    -33.27                                   
REMARK 500 12 ASP A 136      -46.23   -133.63                                   
REMARK 500 12 ASP A 153      -18.61    -49.47                                   
REMARK 500 12 TYR A 161      -29.69    -39.82                                   
REMARK 500 13 LEU A  30      134.14    -36.37                                   
REMARK 500 13 TYR A  73      139.79    -33.39                                   
REMARK 500 13 SER A 106      174.50    -51.48                                   
REMARK 500 13 THR A 110       99.39    -55.17                                   
REMARK 500 13 PRO A 114        3.44    -69.73                                   
REMARK 500 13 VAL A 121      -70.72    -70.40                                   
REMARK 500 13 THR A 130      -74.84    -86.85                                   
REMARK 500 13 LEU A 133       93.63    -33.59                                   
REMARK 500 13 PRO A 138     -179.79    -69.76                                   
REMARK 500 13 ASP A 153      -19.40    -48.50                                   
REMARK 500 13 ILE A 162      -73.35    -83.22                                   
REMARK 500 14 THR A   9       99.03    -43.10                                   
REMARK 500 14 VAL A  12      142.31    -39.28                                   
REMARK 500 14 SER A  13      -72.09   -132.77                                   
REMARK 500 14 LEU A  19      -68.67    -92.05                                   
REMARK 500 14 TYR A  58       39.19    -82.29                                   
REMARK 500 14 TYR A  73      140.35    -34.01                                   
REMARK 500 14 LEU A 101      -19.62    -47.73                                   
REMARK 500 14 GLU A 104      134.33    -35.13                                   
REMARK 500 14 HIS A 105      109.38    -35.38                                   
REMARK 500 14 SER A 106       46.97    -91.84                                   
REMARK 500 14 ILE A 107       37.33     31.24                                   
REMARK 500 14 ASP A 108      -65.89   -133.89                                   
REMARK 500 14 ARG A 116      179.01    -59.00                                   
REMARK 500 14 THR A 130      -75.41    -88.94                                   
REMARK 500 14 LEU A 132       44.03    -78.17                                   
REMARK 500 14 LEU A 133       72.27   -114.68                                   
REMARK 500 14 ASP A 136      -65.98    -99.34                                   
REMARK 500 14 ASP A 137       56.73   -118.03                                   
REMARK 500 14 PRO A 138     -178.75    -69.75                                   
REMARK 500 14 PHE A 154      -30.99    -35.80                                   
REMARK 500 14 TYR A 161      -31.85    -35.15                                   
REMARK 500 14 ILE A 162      -74.24    -65.18                                   
REMARK 500 15 LEU A  19      -61.77    -92.32                                   
REMARK 500 15 GLN A  59      -35.02    -39.30                                   
REMARK 500 15 TYR A  73      139.47    -32.70                                   
REMARK 500 15 TRP A  87       31.37    -84.57                                   
REMARK 500 15 GLU A 104      -70.45    -63.47                                   
REMARK 500 15 HIS A 105       76.33    -65.17                                   
REMARK 500 15 ASP A 108       50.98     73.24                                   
REMARK 500 15 THR A 130      -60.44   -135.01                                   
REMARK 500 15 LEU A 132       44.05    -78.07                                   
REMARK 500 15 ASP A 137       51.93   -117.38                                   
REMARK 500 15 SER A 168       95.91    -36.40                                   
REMARK 500 16 ARG A  10       91.38    -64.09                                   
REMARK 500 16 ARG A  11      144.45    -34.39                                   
REMARK 500 16 VAL A  12      145.63    -33.88                                   
REMARK 500 16 SER A  13      -74.60   -134.90                                   
REMARK 500 16 GLN A  59      -32.08    -36.05                                   
REMARK 500 16 TYR A  73      140.06    -33.03                                   
REMARK 500 16 TRP A  87       39.48    -81.20                                   
REMARK 500 16 ARG A 103      128.03    -39.17                                   
REMARK 500 16 THR A 110      -38.37    -38.45                                   
REMARK 500 16 PRO A 114        0.99    -69.74                                   
REMARK 500 16 ARG A 116     -179.55    -60.68                                   
REMARK 500 16 THR A 130      -77.89    -74.60                                   
REMARK 500 16 LEU A 132       41.83    -81.37                                   
REMARK 500 16 PHE A 135       43.00    -81.04                                   
REMARK 500 16 ILE A 162      -73.48    -77.48                                   
REMARK 500 17 SER A   2       42.21    -93.74                                   
REMARK 500 17 THR A   9      146.43    -37.84                                   
REMARK 500 17 SER A  13      -76.17   -135.02                                   
REMARK 500 17 LYS A  35      117.04   -167.10                                   
REMARK 500 17 ASP A  54       37.34    -99.37                                   
REMARK 500 17 TYR A  73      140.04    -33.78                                   
REMARK 500 17 TRP A  87       35.96    -83.39                                   
REMARK 500 17 PRO A 114        4.43    -69.76                                   
REMARK 500 17 ARG A 116      177.88    -53.85                                   
REMARK 500 17 LEU A 125      -39.71    -38.82                                   
REMARK 500 17 LEU A 133      100.54    -32.72                                   
REMARK 500 17 ILE A 162      -73.44    -73.11                                   
REMARK 500 18 LEU A  19      -68.43    -91.65                                   
REMARK 500 18 ASP A  54       30.16    -92.07                                   
REMARK 500 18 TYR A  58       52.03    -90.08                                   
REMARK 500 18 PRO A  70     -175.67    -69.78                                   
REMARK 500 18 TYR A  73      139.67    -32.87                                   
REMARK 500 18 LEU A 102       32.81    -95.81                                   
REMARK 500 18 ILE A 107       40.46     28.90                                   
REMARK 500 18 THR A 110       30.16     39.04                                   
REMARK 500 18 PRO A 114        4.71    -69.74                                   
REMARK 500 18 THR A 130      -73.11    -94.75                                   
REMARK 500 18 LEU A 132       42.71    -79.75                                   
REMARK 500 18 LEU A 133       62.76   -111.18                                   
REMARK 500 18 PRO A 138     -169.93    -69.75                                   
REMARK 500 18 VAL A 158      -38.33    -37.67                                   
REMARK 500 19 ARG A  10       40.75   -106.12                                   
REMARK 500 19 ARG A  11       40.61   -103.11                                   
REMARK 500 19 SER A  13      -75.31   -114.42                                   
REMARK 500 19 VAL A  23      -39.90    -35.74                                   
REMARK 500 19 PRO A  70     -175.70    -69.76                                   
REMARK 500 19 TYR A  73      139.34    -33.64                                   
REMARK 500 19 GLU A 104     -174.88    -51.63                                   
REMARK 500 19 HIS A 105      136.98    -33.75                                   
REMARK 500 19 THR A 110      -36.49    -37.32                                   
REMARK 500 19 PRO A 114        3.33    -69.80                                   
REMARK 500 19 THR A 130      -74.86   -133.57                                   
REMARK 500 19 LEU A 132       45.76    -76.76                                   
REMARK 500 19 LEU A 133       72.49   -116.11                                   
REMARK 500 19 PRO A 138     -173.67    -69.77                                   
REMARK 500 19 SER A 172       42.58     39.02                                   
REMARK 500 20 SER A   8       45.81    -95.81                                   
REMARK 500 20 VAL A  12      -49.00    -29.13                                   
REMARK 500 20 VAL A  23      -39.61    -35.25                                   
REMARK 500 20 CYS A  32       33.16    -83.88                                   
REMARK 500 20 TYR A  58       47.48    -83.31                                   
REMARK 500 20 PRO A  70     -173.46    -69.74                                   
REMARK 500 20 TYR A  73      140.76    -34.44                                   
REMARK 500 20 ASN A  74       35.65    -86.09                                   
REMARK 500 20 TRP A  87       38.81    -82.34                                   
REMARK 500 20 LEU A 102       34.90    -95.24                                   
REMARK 500 20 PRO A 114        3.82    -69.71                                   
REMARK 500 20 THR A 130      -76.92   -131.47                                   
REMARK 500 20 LEU A 133      106.49    -29.90                                   
REMARK 500 20 PHE A 135       40.88    -80.07                                   
REMARK 500 20 ASP A 137       48.59     73.24                                   
REMARK 500 20 PRO A 138     -171.44    -69.78                                   
REMARK 500 20 TYR A 161      -33.02    -34.64                                   
REMARK 500 20 ILE A 162      -72.48    -72.55                                   
REMARK 500 20 SER A 171      -61.54    -94.63                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSI002010298.1   RELATED DB: TARGETDB                    
DBREF  2EDI A    8   167  UNP    Q969M7   UBE2F_HUMAN     26    185             
SEQADV 2EDI GLY A    1  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A    2  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A    3  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI GLY A    4  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A    5  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A    6  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI GLY A    7  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A  168  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI GLY A  169  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI PRO A  170  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A  171  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI SER A  172  UNP  Q969M7              EXPRESSION TAG                 
SEQADV 2EDI GLY A  173  UNP  Q969M7              EXPRESSION TAG                 
SEQRES   1 A  173  GLY SER SER GLY SER SER GLY SER THR ARG ARG VAL SER          
SEQRES   2 A  173  VAL ARG ASP LYS LEU LEU VAL LYS GLU VAL ALA GLU LEU          
SEQRES   3 A  173  GLU ALA ASN LEU PRO CYS THR CYS LYS VAL HIS PHE PRO          
SEQRES   4 A  173  ASP PRO ASN LYS LEU HIS CYS PHE GLN LEU THR VAL THR          
SEQRES   5 A  173  PRO ASP GLU GLY TYR TYR GLN GLY GLY LYS PHE GLN PHE          
SEQRES   6 A  173  GLU THR GLU VAL PRO ASP ALA TYR ASN MET VAL PRO PRO          
SEQRES   7 A  173  LYS VAL LYS CYS LEU THR LYS ILE TRP HIS PRO ASN ILE          
SEQRES   8 A  173  THR GLU THR GLY GLU ILE CYS LEU SER LEU LEU ARG GLU          
SEQRES   9 A  173  HIS SER ILE ASP GLY THR GLY TRP ALA PRO THR ARG THR          
SEQRES  10 A  173  LEU LYS ASP VAL VAL TRP GLY LEU ASN SER LEU PHE THR          
SEQRES  11 A  173  ASP LEU LEU ASN PHE ASP ASP PRO LEU ASN ILE GLU ALA          
SEQRES  12 A  173  ALA GLU HIS HIS LEU ARG ASP LYS GLU ASP PHE ARG ASN          
SEQRES  13 A  173  LYS VAL ASP ASP TYR ILE LYS ARG TYR ALA ARG SER GLY          
SEQRES  14 A  173  PRO SER SER GLY                                              
HELIX    1   1 SER A   13  LEU A   30  1                                  18    
HELIX    2   2 THR A  117  LEU A  128  1                                  12    
HELIX    3   3 ASN A  140  ASP A  150  1                                  11    
HELIX    4   4 ASP A  150  ALA A  166  1                                  17    
SHEET    1   A 4 CYS A  34  HIS A  37  0                                        
SHEET    2   A 4 PHE A  47  VAL A  51 -1  O  GLN A  48   N  HIS A  37           
SHEET    3   A 4 PHE A  63  GLU A  68 -1  O  PHE A  65   N  LEU A  49           
SHEET    4   A 4 LYS A  79  CYS A  82 -1  O  LYS A  81   N  GLU A  66           
CISPEP   1 TYR A   73    ASN A   74          1         0.01                     
CISPEP   2 TYR A   73    ASN A   74          2         0.02                     
CISPEP   3 TYR A   73    ASN A   74          3         0.00                     
CISPEP   4 TYR A   73    ASN A   74          4        -0.06                     
CISPEP   5 TYR A   73    ASN A   74          5         0.03                     
CISPEP   6 TYR A   73    ASN A   74          6         0.03                     
CISPEP   7 TYR A   73    ASN A   74          7        -0.05                     
CISPEP   8 TYR A   73    ASN A   74          8        -0.02                     
CISPEP   9 TYR A   73    ASN A   74          9        -0.05                     
CISPEP  10 TYR A   73    ASN A   74         10        -0.08                     
CISPEP  11 TYR A   73    ASN A   74         11        -0.10                     
CISPEP  12 TYR A   73    ASN A   74         12         0.00                     
CISPEP  13 TYR A   73    ASN A   74         13         0.02                     
CISPEP  14 TYR A   73    ASN A   74         14         0.01                     
CISPEP  15 TYR A   73    ASN A   74         15        -0.02                     
CISPEP  16 TYR A   73    ASN A   74         16        -0.04                     
CISPEP  17 TYR A   73    ASN A   74         17         0.03                     
CISPEP  18 TYR A   73    ASN A   74         18        -0.01                     
CISPEP  19 TYR A   73    ASN A   74         19         0.07                     
CISPEP  20 TYR A   73    ASN A   74         20         0.01                     
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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