HEADER TRANSCRIPTION 28-MAR-07 2EN3
TITLE SOLUTION STRUCTURE OF THE C2H2 TYPE ZINC FINGER (REGION 796-828) OF
TITLE 2 HUMAN ZINC FINGER PROTEIN 95 HOMOLOG
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: ZINC FINGER PROTEIN 95 HOMOLOG;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: ZF-C2H2;
COMPND 5 SYNONYM: ZFP-95;
COMPND 6 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: ZFP95;
SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: P061225-16;
SOURCE 8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS
KEYWDS ZF-C2H2, STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT ON PROTEIN
KEYWDS 2 STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL
KEYWDS 3 GENOMICS/PROTEOMICS INITIATIVE, RSGI, TRANSCRIPTION
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR N.TOCHIO,T.TOMIZAWA,H.ABE,K.SAITO,H.LI,M.SATO,S.KOSHIBA,N.KOBAYASHI,
AUTHOR 2 T.KIGAWA,S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE
AUTHOR 3 (RSGI)
REVDAT 3 09-MAR-22 2EN3 1 REMARK SEQADV
REVDAT 2 24-FEB-09 2EN3 1 VERSN
REVDAT 1 02-OCT-07 2EN3 0
JRNL AUTH N.TOCHIO,T.TOMIZAWA,H.ABE,K.SAITO,H.LI,M.SATO,S.KOSHIBA,
JRNL AUTH 2 N.KOBAYASHI,T.KIGAWA,S.YOKOYAMA
JRNL TITL SOLUTION STRUCTURE OF THE C2H2 TYPE ZINC FINGER (REGION
JRNL TITL 2 796-828) OF HUMAN ZINC FINGER PROTEIN 95 HOMOLOG
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : XWINNMR 3.5, CYANA 2.0.17
REMARK 3 AUTHORS : BRUKER (XWINNMR), GUNTERT, P. (CYANA)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2EN3 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 30-MAY-07.
REMARK 100 THE DEPOSITION ID IS D_1000026866.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 296
REMARK 210 PH : 7.0
REMARK 210 IONIC STRENGTH : 120MM
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1.0MM SAMPLE U-15N,13C; 20MM D
REMARK 210 -TRIS-HCL; 100MM NACL; 0.05MM
REMARK 210 ZNCL2; 1MM IDA; 1MM D-DTT; 0.02%
REMARK 210 NAN3; 90% H2O, 10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_13C-SEPARATED_NOESY; 3D_15N
REMARK 210 -SEPARATED_NOESY
REMARK 210 SPECTROMETER FIELD STRENGTH : 800 MHZ
REMARK 210 SPECTROMETER MODEL : AVANCE
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NMRPIPE 20030801, NMRVIEW 5.0.4,
REMARK 210 KUJIRA 0.9820, CYANA 2.0.17
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LEAST
REMARK 210 RESTRAINT VIOLATIONS, TARGET
REMARK 210 FUNCTION
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 SER A 2 89.20 -67.64
REMARK 500 1 ARG A 37 -61.94 -90.03
REMARK 500 1 SER A 41 38.09 72.90
REMARK 500 1 PRO A 43 92.33 -69.73
REMARK 500 2 PRO A 40 87.63 -69.76
REMARK 500 3 SER A 6 42.60 36.70
REMARK 500 3 ARG A 37 -61.15 -93.84
REMARK 500 4 THR A 8 141.28 -37.28
REMARK 500 4 SER A 23 33.06 -89.54
REMARK 500 4 PRO A 40 2.83 -69.78
REMARK 500 4 PRO A 43 86.72 -69.70
REMARK 500 4 SER A 44 103.35 -59.04
REMARK 500 4 SER A 45 -62.71 -108.09
REMARK 500 5 PRO A 40 0.88 -69.69
REMARK 500 6 SER A 34 -19.43 -48.32
REMARK 500 6 PRO A 40 87.21 -69.78
REMARK 500 6 PRO A 43 85.35 -69.80
REMARK 500 6 SER A 44 102.28 -42.71
REMARK 500 7 SER A 3 113.02 -161.63
REMARK 500 7 SER A 5 46.88 -87.30
REMARK 500 7 SER A 6 48.12 -83.45
REMARK 500 7 SER A 23 34.58 -85.95
REMARK 500 8 PRO A 40 99.35 -69.79
REMARK 500 8 SER A 45 110.31 -165.00
REMARK 500 9 SER A 23 33.63 -85.32
REMARK 500 9 PRO A 40 1.42 -69.73
REMARK 500 9 PRO A 43 -165.41 -69.84
REMARK 500 10 PRO A 43 87.31 -69.80
REMARK 500 10 SER A 45 118.78 -172.54
REMARK 500 11 SER A 45 43.64 37.77
REMARK 500 13 PRO A 40 -174.04 -69.74
REMARK 500 13 SER A 41 121.68 -174.31
REMARK 500 13 SER A 44 42.67 35.82
REMARK 500 13 SER A 45 118.01 -37.19
REMARK 500 14 SER A 44 155.73 -41.50
REMARK 500 16 THR A 38 150.41 -46.12
REMARK 500 16 PRO A 40 93.82 -69.80
REMARK 500 16 PRO A 43 97.17 -69.76
REMARK 500 16 SER A 45 42.06 35.76
REMARK 500 18 SER A 2 43.21 -94.18
REMARK 500 18 SER A 23 34.23 -86.40
REMARK 500 18 SER A 34 -19.96 -47.38
REMARK 500 18 PRO A 40 -169.32 -69.81
REMARK 500 18 SER A 45 146.85 -171.81
REMARK 500 19 SER A 2 42.21 39.81
REMARK 500 19 THR A 38 127.86 -33.33
REMARK 500 19 SER A 41 43.78 -101.36
REMARK 500 20 SER A 3 -45.59 -131.72
REMARK 500 20 SER A 5 -52.42 -125.15
REMARK 500 20 SER A 34 -19.11 -49.40
REMARK 500
REMARK 500 REMARK: NULL
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR: M RES CSSEQI METAL
REMARK 620 ZN A 201 ZN
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 CYS A 15 SG
REMARK 620 2 CYS A 18 SG 116.6
REMARK 620 3 HIS A 31 NE2 108.0 94.2
REMARK 620 4 HIS A 35 NE2 116.2 117.8 98.7
REMARK 620 N 1 2 3
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: HSO003005608.8 RELATED DB: TARGETDB
DBREF 2EN3 A 8 40 UNP Q9Y2L8 ZFP95_HUMAN 796 828
SEQADV 2EN3 GLY A 1 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 2 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 3 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 GLY A 4 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 5 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 6 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 GLY A 7 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 41 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 GLY A 42 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 PRO A 43 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 44 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 SER A 45 UNP Q9Y2L8 EXPRESSION TAG
SEQADV 2EN3 GLY A 46 UNP Q9Y2L8 EXPRESSION TAG
SEQRES 1 A 46 GLY SER SER GLY SER SER GLY THR GLY GLU LYS PRO PHE
SEQRES 2 A 46 GLN CYS LYS GLU CYS GLY MET ASN PHE SER TRP SER CYS
SEQRES 3 A 46 SER LEU PHE LYS HIS LEU ARG SER HIS GLU ARG THR ASP
SEQRES 4 A 46 PRO SER GLY PRO SER SER GLY
HET ZN A 201 1
HETNAM ZN ZINC ION
FORMUL 2 ZN ZN 2+
HELIX 1 1 SER A 25 THR A 38 1 14
SHEET 1 A 2 PHE A 13 GLN A 14 0
SHEET 2 A 2 ASN A 21 PHE A 22 -1 O PHE A 22 N PHE A 13
LINK SG CYS A 15 ZN ZN A 201 1555 1555 2.37
LINK SG CYS A 18 ZN ZN A 201 1555 1555 2.33
LINK NE2 HIS A 31 ZN ZN A 201 1555 1555 2.36
LINK NE2 HIS A 35 ZN ZN A 201 1555 1555 2.34
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END