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Database: PDB
Entry: 2ENQ
LinkDB: 2ENQ
Original site: 2ENQ 
HEADER    TRANSFERASE                             28-MAR-07   2ENQ              
TITLE     SOLUTION STRUCTURE OF THE C2 DOMAIN FROM HUMAN PI3-KINASE             
TITLE    2 P110 SUBUNIT ALPHA                                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 3-KINASE             
COMPND   3 CATALYTIC SUBUNIT ALPHA ISOFORM;                                     
COMPND   4 CHAIN: A;                                                            
COMPND   5 FRAGMENT: C2 DOMAIN;                                                 
COMPND   6 SYNONYM: PI3-KINASE P110 SUBUNIT ALPHA, PTDINS-3- KINASE             
COMPND   7 P110, PI3K;                                                          
COMPND   8 EC: 2.7.1.153;                                                       
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: PIK3CA;                                                        
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P060417-17;                               
SOURCE   8 OTHER_DETAILS: CELL FREE PROTEIN SYNTHESIS                           
KEYWDS    PI3-KINASE P110 SUBUNIT ALPHA,PHOSPHATIDYLINOSITOL-4,5-               
KEYWDS   2 BISPHOSPHATE 3-KINASE CATALYTIC SUBUNIT ALPHA ISOFORM,PI3K,          
KEYWDS   3 C2 TYPE2,BETA SANDWICHX, STRUCTURAL GENOMICS, NPPSFA,                
KEYWDS   4 NATIONAL PROJECT ON PROTEIN STRUCTURAL AND FUNCTIONAL                
KEYWDS   5 ANALYSES, RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE,           
KEYWDS   6 RSGI, TRANSFERASE                                                    
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    H.ENDO,T.NAGASHIMA,M.YOSHIDA,F.HAYASHI,S.YOKOYAMA,RIKEN               
AUTHOR   2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)                     
REVDAT   2   24-FEB-09 2ENQ    1       VERSN                                    
REVDAT   1   01-APR-08 2ENQ    0                                                
JRNL        AUTH   H.ENDO,T.NAGASHIMA,M.YOSHIDA,F.HAYASHI,S.YOKOYAMA            
JRNL        TITL   SOLUTION STRUCTURE OF THE C2 DOMAIN FROM HUMAN               
JRNL        TITL 2 PI3-KINASE P110 SUBUNIT ALPHA                                
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2ENQ COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 01-JUN-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB026885.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 293                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.77MM 13C, 15N-LABELED            
REMARK 210                                   PROTEIN; 20MM D-TRIS-              
REMARK 210                                   HCL(PH7.0), 100MM NACL; 1MM D-     
REMARK 210                                   DTT; 0.02% NAN3; 10% D2O; 90%      
REMARK 210                                   H2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 900 MHZ, 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1C, NMRPIPE 20060702,       
REMARK 210                                   NMRVIEW 5.0.4, KUJIRA 0.9820,      
REMARK 210                                   CYANA 2.0.17                       
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS,TARGET        
REMARK 210                                   FUNCTION                           
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   6       36.07     34.49                                   
REMARK 500  1 LEU A  16      -69.19   -100.87                                   
REMARK 500  1 TYR A  20       52.16     39.47                                   
REMARK 500  1 ASN A  22       34.31    -83.79                                   
REMARK 500  1 ASN A  24      -74.66    -79.38                                   
REMARK 500  1 ILE A  25      173.22    -46.09                                   
REMARK 500  1 GLN A  51      170.39    -52.75                                   
REMARK 500  1 PRO A  58        3.31    -69.75                                   
REMARK 500  1 TRP A  60      -63.46   -106.22                                   
REMARK 500  1 ASP A  67       38.08    -82.49                                   
REMARK 500  1 PRO A  71        2.94    -69.74                                   
REMARK 500  1 ASN A 105       98.91    -68.87                                   
REMARK 500  1 ASP A 108      170.22    -51.65                                   
REMARK 500  1 LEU A 113      132.95    -34.20                                   
REMARK 500  1 LYS A 117      150.44    -43.01                                   
REMARK 500  1 HIS A 127      110.38    -35.25                                   
REMARK 500  1 LEU A 129      105.80    -55.61                                   
REMARK 500  2 SER A   9      -35.86   -130.01                                   
REMARK 500  2 LEU A  16      -73.66   -100.23                                   
REMARK 500  2 TYR A  20       45.04     35.59                                   
REMARK 500  2 VAL A  23       99.83    -53.23                                   
REMARK 500  2 ASN A  24      -62.14    -94.91                                   
REMARK 500  2 ILE A  25     -174.95    -64.61                                   
REMARK 500  2 PRO A  58        3.73    -69.75                                   
REMARK 500  2 TRP A  60      -68.45   -120.88                                   
REMARK 500  2 ASP A  67       30.29    -84.33                                   
REMARK 500  2 PRO A  71        2.43    -69.73                                   
REMARK 500  2 ASP A 108     -176.20    -56.75                                   
REMARK 500  2 LEU A 129       94.69    -41.06                                   
REMARK 500  2 VAL A 138      176.87    -56.85                                   
REMARK 500  3 SER A   6      165.08    -42.57                                   
REMARK 500  3 SER A   9      -36.81   -132.02                                   
REMARK 500  3 LEU A  16      -73.22    -97.35                                   
REMARK 500  3 ASN A  24      -73.49    -98.47                                   
REMARK 500  3 ARG A  26       57.93   -118.49                                   
REMARK 500  3 ILE A  28      123.45    -39.42                                   
REMARK 500  3 GLN A  51      179.88    -49.95                                   
REMARK 500  3 PRO A  58        3.63    -69.75                                   
REMARK 500  3 TRP A  60      -56.35   -128.93                                   
REMARK 500  3 ASN A  61       37.93     38.37                                   
REMARK 500  3 ASP A  67       47.66    -81.34                                   
REMARK 500  3 PRO A  71        3.11    -69.82                                   
REMARK 500  3 LYS A  90      -72.83    -44.28                                   
REMARK 500  3 LEU A 106      -34.85    -35.08                                   
REMARK 500  3 ASP A 108     -178.10    -52.92                                   
REMARK 500  3 THR A 110       33.86    -93.99                                   
REMARK 500  3 LEU A 113      140.06    -34.66                                   
REMARK 500  3 HIS A 127      108.84    -42.46                                   
REMARK 500  3 GLU A 146       34.56    -84.30                                   
REMARK 500  4 LEU A  16      -73.00   -102.06                                   
REMARK 500  4 ASN A  22       38.69    -84.56                                   
REMARK 500  4 ASN A  24      -73.38    -72.44                                   
REMARK 500  4 ASP A  46      151.49    -47.84                                   
REMARK 500  4 GLN A  51      154.49    -46.85                                   
REMARK 500  4 PRO A  58        3.27    -69.79                                   
REMARK 500  4 ASN A  61       49.72     36.61                                   
REMARK 500  4 PRO A  71        2.84    -69.80                                   
REMARK 500  4 LEU A  99      -53.36   -127.82                                   
REMARK 500  4 ASN A 105       96.12    -66.21                                   
REMARK 500  4 ASP A 108      174.35    -48.06                                   
REMARK 500  4 LEU A 113      146.25    -34.69                                   
REMARK 500  4 LEU A 129      110.11    -34.63                                   
REMARK 500  4 GLU A 130       56.32   -101.37                                   
REMARK 500  4 ASN A 142      133.33    -37.09                                   
REMARK 500  4 ASN A 144      126.38    -37.12                                   
REMARK 500  5 SER A   6     -178.48    -69.06                                   
REMARK 500  5 SER A   9      -43.84   -130.17                                   
REMARK 500  5 LEU A  16      -71.19    -98.04                                   
REMARK 500  5 CYS A  17     -179.54   -171.06                                   
REMARK 500  5 ASN A  22       36.89    -89.11                                   
REMARK 500  5 ASN A  24      -70.70    -96.92                                   
REMARK 500  5 ASP A  46      155.13    -47.84                                   
REMARK 500  5 PRO A  58        3.57    -69.77                                   
REMARK 500  5 TRP A  60      -60.58    -97.91                                   
REMARK 500  5 ASP A  67       32.50    -83.80                                   
REMARK 500  5 PRO A  71        2.14    -69.79                                   
REMARK 500  5 LYS A  90      -70.76    -41.87                                   
REMARK 500  5 LEU A  99      -53.09   -128.51                                   
REMARK 500  5 LEU A 106      -39.99    -36.49                                   
REMARK 500  5 ASP A 108     -174.85    -57.02                                   
REMARK 500  5 LEU A 113      135.14    -34.70                                   
REMARK 500  5 PRO A 126     -165.27    -69.72                                   
REMARK 500  5 ASN A 142      120.48    -34.40                                   
REMARK 500  5 THR A 147      150.22    -48.28                                   
REMARK 500  6 SER A   9      -43.37   -132.64                                   
REMARK 500  6 LEU A  16      -71.99    -98.44                                   
REMARK 500  6 ASN A  24      -74.82   -109.71                                   
REMARK 500  6 ARG A  26       74.63   -112.77                                   
REMARK 500  6 ASP A  27      -46.02   -134.98                                   
REMARK 500  6 ILE A  28      123.21    -38.50                                   
REMARK 500  6 GLN A  51      161.97    -41.72                                   
REMARK 500  6 PRO A  58        3.09    -69.74                                   
REMARK 500  6 ASP A  67       44.25    -80.08                                   
REMARK 500  6 PRO A  71        2.98    -69.82                                   
REMARK 500  6 LEU A  99      -50.21   -123.87                                   
REMARK 500  6 ASN A 105       89.42    -64.66                                   
REMARK 500  6 ASP A 108      173.09    -52.13                                   
REMARK 500  6 LEU A 113      133.21    -34.94                                   
REMARK 500  6 LEU A 129      126.67    -33.56                                   
REMARK 500  6 ASP A 131     -175.83    -53.54                                   
REMARK 500  6 ASN A 144      135.95    -39.01                                   
REMARK 500  6 GLU A 146       41.39    -85.19                                   
REMARK 500  7 LEU A  16      -72.23   -101.89                                   
REMARK 500  7 ASN A  24      -51.33   -122.41                                   
REMARK 500  7 ARG A  26       50.37   -115.43                                   
REMARK 500  7 ILE A  28      132.34    -36.52                                   
REMARK 500  7 CYS A  45      -67.07   -133.80                                   
REMARK 500  7 GLN A  51      172.74    -49.92                                   
REMARK 500  7 PRO A  58        3.17    -69.74                                   
REMARK 500  7 ASN A  61       41.21     39.30                                   
REMARK 500  7 ASP A  67       45.52    -78.05                                   
REMARK 500  7 PRO A  71        2.42    -69.71                                   
REMARK 500  7 LYS A  90      -74.18    -49.25                                   
REMARK 500  7 ASN A 105       95.79    -67.28                                   
REMARK 500  7 ASP A 108     -177.65    -52.19                                   
REMARK 500  7 THR A 110       34.70    -88.73                                   
REMARK 500  7 ASP A 111       54.75     35.62                                   
REMARK 500  7 LEU A 113      138.79    -34.36                                   
REMARK 500  7 GLU A 146       40.39    -82.16                                   
REMARK 500  8 ASN A   8      135.17    -35.61                                   
REMARK 500  8 LEU A  16      -72.29    -99.26                                   
REMARK 500  8 TYR A  20       44.60     39.54                                   
REMARK 500  8 ASN A  22       47.19    -82.94                                   
REMARK 500  8 ASP A  46      151.42    -41.36                                   
REMARK 500  8 GLN A  51      174.76    -49.67                                   
REMARK 500  8 PRO A  58        2.97    -69.80                                   
REMARK 500  8 ARG A  59      109.55    -50.80                                   
REMARK 500  8 ASN A  61       41.05     35.25                                   
REMARK 500  8 ASP A  67       40.50    -82.21                                   
REMARK 500  8 PRO A  71        3.49    -69.74                                   
REMARK 500  8 ASN A 105       96.37    -62.41                                   
REMARK 500  8 ASP A 108     -176.21    -55.64                                   
REMARK 500  8 LYS A 117      148.84    -39.63                                   
REMARK 500  8 LEU A 129       99.91    -34.73                                   
REMARK 500  8 VAL A 138      174.98    -59.32                                   
REMARK 500  9 SER A   3      178.05    -59.74                                   
REMARK 500  9 SER A   9      -47.13   -133.69                                   
REMARK 500  9 LEU A  16      -70.63   -102.47                                   
REMARK 500  9 CYS A  17     -176.82   -172.45                                   
REMARK 500  9 ASN A  24      -61.99   -104.68                                   
REMARK 500  9 PRO A  58        3.66    -69.78                                   
REMARK 500  9 ARG A  59      108.35    -55.64                                   
REMARK 500  9 ASP A  67       47.61    -82.71                                   
REMARK 500  9 LEU A 106      -36.01    -34.49                                   
REMARK 500  9 ASP A 108     -175.82    -53.10                                   
REMARK 500  9 LEU A 113      141.83    -36.07                                   
REMARK 500  9 PRO A 143        1.98    -69.74                                   
REMARK 500 10 LEU A  16      -67.74   -103.71                                   
REMARK 500 10 ASN A  22       35.51    -84.03                                   
REMARK 500 10 GLN A  51      176.77    -54.15                                   
REMARK 500 10 PRO A  58        2.94    -69.77                                   
REMARK 500 10 ARG A  59      103.84    -58.47                                   
REMARK 500 10 ASN A  61       46.99     39.23                                   
REMARK 500 10 ASP A  67       41.74    -80.20                                   
REMARK 500 10 PRO A  71        3.49    -69.70                                   
REMARK 500 10 LEU A  99      -51.13   -126.37                                   
REMARK 500 10 LEU A 106      -35.78    -35.14                                   
REMARK 500 10 ASP A 108     -177.06    -54.93                                   
REMARK 500 10 THR A 110       32.14    -86.90                                   
REMARK 500 10 ASP A 111       54.01     37.78                                   
REMARK 500 10 VAL A 114      176.56    -57.51                                   
REMARK 500 10 LYS A 117      158.04    -39.55                                   
REMARK 500 10 PRO A 126     -173.86    -69.78                                   
REMARK 500 10 GLU A 146       39.30    -83.26                                   
REMARK 500 11 SER A   5      -53.89   -124.96                                   
REMARK 500 11 LEU A  16      -72.27    -94.23                                   
REMARK 500 11 VAL A  21      161.78    -40.42                                   
REMARK 500 11 ASN A  22       37.24    -84.53                                   
REMARK 500 11 PRO A  58        4.27    -69.77                                   
REMARK 500 11 TRP A  60      -65.36    -95.46                                   
REMARK 500 11 PRO A  71        3.56    -69.81                                   
REMARK 500 11 LEU A  99      -56.91   -122.13                                   
REMARK 500 11 ASN A 105       92.49    -59.21                                   
REMARK 500 11 ASP A 108     -176.22    -53.13                                   
REMARK 500 11 THR A 110       33.31    -86.22                                   
REMARK 500 11 ASP A 111       49.90     37.18                                   
REMARK 500 11 LEU A 113      138.21    -34.78                                   
REMARK 500 11 LYS A 117      151.15    -43.12                                   
REMARK 500 11 VAL A 138     -178.38    -50.43                                   
REMARK 500 11 PRO A 143        2.85    -69.73                                   
REMARK 500 12 SER A   3      170.36    -47.07                                   
REMARK 500 12 LEU A  16      -74.07    -99.61                                   
REMARK 500 12 VAL A  23      110.65    -35.02                                   
REMARK 500 12 PRO A  58        3.23    -69.81                                   
REMARK 500 12 ASP A  67       43.69    -80.34                                   
REMARK 500 12 PRO A  71        3.04    -69.75                                   
REMARK 500 12 LEU A 106      -35.25    -34.90                                   
REMARK 500 12 ASP A 108     -176.37    -51.07                                   
REMARK 500 12 ASP A 111       34.38     70.61                                   
REMARK 500 12 LEU A 113      149.88    -38.58                                   
REMARK 500 12 VAL A 138      179.69    -56.02                                   
REMARK 500 12 GLU A 146       39.69    -84.35                                   
REMARK 500 13 SER A   9      -67.14   -134.59                                   
REMARK 500 13 LEU A  16      -74.68    -98.18                                   
REMARK 500 13 ASP A  27      -53.69   -132.43                                   
REMARK 500 13 ILE A  28      122.71    -39.09                                   
REMARK 500 13 TYR A  32      149.23   -173.02                                   
REMARK 500 13 GLN A  51      173.25    -55.91                                   
REMARK 500 13 PRO A  58        4.35    -69.80                                   
REMARK 500 13 ARG A  59      107.29    -58.06                                   
REMARK 500 13 ASN A  61       43.38     39.48                                   
REMARK 500 13 ASP A  67       52.32    -90.34                                   
REMARK 500 13 LYS A  90      104.49    -47.49                                   
REMARK 500 13 ASN A 105       95.87    -67.11                                   
REMARK 500 13 LEU A 106      -39.84    -34.89                                   
REMARK 500 13 ASP A 108     -176.87    -52.22                                   
REMARK 500 13 PRO A 126     -169.45    -69.72                                   
REMARK 500 14 SER A   9      -39.22   -132.59                                   
REMARK 500 14 LEU A  16      -72.96   -100.49                                   
REMARK 500 14 VAL A  23      100.86    -34.79                                   
REMARK 500 14 ASN A  24      -69.64   -103.81                                   
REMARK 500 14 ILE A  25     -179.12    -49.56                                   
REMARK 500 14 ASP A  27      -43.87   -133.44                                   
REMARK 500 14 ILE A  28      133.23    -37.88                                   
REMARK 500 14 TYR A  32      138.57   -173.67                                   
REMARK 500 14 PRO A  58        3.85    -69.72                                   
REMARK 500 14 ARG A  59      109.16    -52.63                                   
REMARK 500 14 ASN A  61       44.59     35.30                                   
REMARK 500 14 ASP A  67       44.26    -88.21                                   
REMARK 500 14 PRO A  71        2.21    -69.77                                   
REMARK 500 14 LEU A 106      -33.61    -36.26                                   
REMARK 500 14 ASP A 108     -178.05    -50.86                                   
REMARK 500 14 THR A 110       30.23    -91.25                                   
REMARK 500 14 LEU A 113      148.09    -36.73                                   
REMARK 500 14 PRO A 126     -175.40    -69.75                                   
REMARK 500 14 PRO A 143        2.03    -69.72                                   
REMARK 500 15 LEU A  16      -72.48    -96.37                                   
REMARK 500 15 VAL A  23      109.55    -36.29                                   
REMARK 500 15 ASN A  24      -67.27   -128.50                                   
REMARK 500 15 ARG A  26       52.69   -111.57                                   
REMARK 500 15 CYS A  45      -69.57   -132.61                                   
REMARK 500 15 GLN A  51      178.70    -48.93                                   
REMARK 500 15 PRO A  58        1.39    -69.78                                   
REMARK 500 15 ASN A  61       42.50     34.00                                   
REMARK 500 15 ASP A  67       38.31    -84.25                                   
REMARK 500 15 PRO A  71        2.16    -69.75                                   
REMARK 500 15 LEU A  99      -52.72   -128.48                                   
REMARK 500 15 ASN A 105       99.55    -66.50                                   
REMARK 500 15 ASP A 108      174.92    -54.64                                   
REMARK 500 15 PRO A 126     -178.18    -69.72                                   
REMARK 500 15 LEU A 129      103.99    -48.74                                   
REMARK 500 15 VAL A 138      175.30    -59.47                                   
REMARK 500 16 SER A   9      -50.95   -133.51                                   
REMARK 500 16 LEU A  16      -74.19   -103.35                                   
REMARK 500 16 ASN A  22       34.63     72.58                                   
REMARK 500 16 ILE A  25     -177.93    -61.31                                   
REMARK 500 16 GLN A  51      174.69    -51.39                                   
REMARK 500 16 PRO A  58        2.99    -69.72                                   
REMARK 500 16 TRP A  60      -63.81   -105.74                                   
REMARK 500 16 ASP A  67       41.29    -81.06                                   
REMARK 500 16 PRO A  71        3.02    -69.81                                   
REMARK 500 16 ASN A 105       96.63    -64.27                                   
REMARK 500 16 LEU A 106      -35.65    -38.75                                   
REMARK 500 16 ASP A 108      175.09    -51.09                                   
REMARK 500 16 LEU A 113      143.52    -38.03                                   
REMARK 500 16 PRO A 126     -164.80    -69.80                                   
REMARK 500 16 VAL A 138      173.77    -54.78                                   
REMARK 500 17 LEU A  16      -72.82   -100.85                                   
REMARK 500 17 TYR A  20       38.75     72.69                                   
REMARK 500 17 ASP A  27      -64.85    -92.00                                   
REMARK 500 17 ILE A  28      123.66    -35.16                                   
REMARK 500 17 GLN A  51      177.37    -51.92                                   
REMARK 500 17 ASN A  61       51.58     33.99                                   
REMARK 500 17 ASP A  67       42.34    -79.90                                   
REMARK 500 17 LEU A 106      -38.68    -34.43                                   
REMARK 500 17 LEU A 113      125.13    -34.05                                   
REMARK 500 17 VAL A 114      176.26    -58.20                                   
REMARK 500 17 PRO A 126     -174.03    -69.80                                   
REMARK 500 17 GLU A 146       39.62    -83.41                                   
REMARK 500 18 LEU A  16      -72.40   -100.45                                   
REMARK 500 18 VAL A  23      106.23    -35.89                                   
REMARK 500 18 ASN A  24      -60.10    -95.63                                   
REMARK 500 18 GLN A  51      172.72    -49.71                                   
REMARK 500 18 PRO A  58        2.23    -69.73                                   
REMARK 500 18 ARG A  59      105.25    -54.20                                   
REMARK 500 18 PRO A  71        2.86    -69.75                                   
REMARK 500 18 LEU A  99      -55.04   -122.72                                   
REMARK 500 18 ASN A 105       87.51    -64.87                                   
REMARK 500 18 LEU A 106      -38.66    -33.46                                   
REMARK 500 18 ASP A 108      177.38    -56.75                                   
REMARK 500 18 THR A 110       34.12    -86.57                                   
REMARK 500 18 ASP A 111       49.49     38.22                                   
REMARK 500 18 LEU A 113      134.12    -34.19                                   
REMARK 500 19 SER A   9      -33.12   -130.17                                   
REMARK 500 19 LEU A  16      -75.15    -94.83                                   
REMARK 500 19 ASN A  24      -73.62    -85.72                                   
REMARK 500 19 ILE A  25      179.65    -51.96                                   
REMARK 500 19 ASP A  27      -33.81   -133.93                                   
REMARK 500 19 ASP A  46      153.80    -41.91                                   
REMARK 500 19 GLN A  51      178.42    -53.39                                   
REMARK 500 19 PRO A  58        3.29    -69.77                                   
REMARK 500 19 TRP A  60      -68.53    -95.07                                   
REMARK 500 19 PRO A  71        2.60    -69.79                                   
REMARK 500 19 ASN A 105       94.30    -65.09                                   
REMARK 500 19 ASP A 108     -175.96    -56.48                                   
REMARK 500 19 THR A 110       32.94    -88.80                                   
REMARK 500 19 LEU A 113      135.16    -34.11                                   
REMARK 500 19 THR A 147      151.56    -47.13                                   
REMARK 500 19 ASP A 155      142.90   -172.79                                   
REMARK 500 20 SER A   3      -61.87   -101.47                                   
REMARK 500 20 LEU A  16      -73.51    -95.06                                   
REMARK 500 20 VAL A  23      101.72    -34.90                                   
REMARK 500 20 ILE A  25      177.28    -48.07                                   
REMARK 500 20 ASP A  27      -50.10   -134.74                                   
REMARK 500 20 ILE A  28      136.23    -33.91                                   
REMARK 500 20 PRO A  43       82.99    -69.77                                   
REMARK 500 20 PRO A  58        3.51    -69.73                                   
REMARK 500 20 ASN A  61       44.57     33.76                                   
REMARK 500 20 ASP A  67       44.08    -87.63                                   
REMARK 500 20 PRO A  71        1.64    -69.75                                   
REMARK 500 20 ASN A 105       96.14    -66.76                                   
REMARK 500 20 LEU A 106      -39.42    -35.00                                   
REMARK 500 20 ASP A 108      173.82    -57.48                                   
REMARK 500 20 LEU A 113      139.84    -34.23                                   
REMARK 500 20 LEU A 129      110.87    -37.53                                   
REMARK 500 20 VAL A 138      172.77    -52.81                                   
REMARK 500 20 PRO A 143        1.49    -69.78                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSO002000026.1   RELATED DB: TARGETDB                    
DBREF  2ENQ A    8   158  UNP    P42336   PK3CA_HUMAN    331    481             
SEQADV 2ENQ GLY A    1  UNP  P42336              EXPRESSION TAG                 
SEQADV 2ENQ SER A    2  UNP  P42336              EXPRESSION TAG                 
SEQADV 2ENQ SER A    3  UNP  P42336              EXPRESSION TAG                 
SEQADV 2ENQ GLY A    4  UNP  P42336              EXPRESSION TAG                 
SEQADV 2ENQ SER A    5  UNP  P42336              EXPRESSION TAG                 
SEQADV 2ENQ SER A    6  UNP  P42336              EXPRESSION TAG                 
SEQADV 2ENQ GLY A    7  UNP  P42336              EXPRESSION TAG                 
SEQRES   1 A  158  GLY SER SER GLY SER SER GLY ASN SER ALA LEU ARG ILE          
SEQRES   2 A  158  LYS ILE LEU CYS ALA THR TYR VAL ASN VAL ASN ILE ARG          
SEQRES   3 A  158  ASP ILE ASP LYS ILE TYR VAL ARG THR GLY ILE TYR HIS          
SEQRES   4 A  158  GLY GLY GLU PRO LEU CYS ASP ASN VAL ASN THR GLN ARG          
SEQRES   5 A  158  VAL PRO CYS SER ASN PRO ARG TRP ASN GLU TRP LEU ASN          
SEQRES   6 A  158  TYR ASP ILE TYR ILE PRO ASP LEU PRO ARG ALA ALA ARG          
SEQRES   7 A  158  LEU CYS LEU SER ILE CYS SER VAL LYS GLY ARG LYS GLY          
SEQRES   8 A  158  ALA LYS GLU GLU HIS CYS PRO LEU ALA TRP GLY ASN ILE          
SEQRES   9 A  158  ASN LEU PHE ASP TYR THR ASP THR LEU VAL SER GLY LYS          
SEQRES  10 A  158  MET ALA LEU ASN LEU TRP PRO VAL PRO HIS GLY LEU GLU          
SEQRES  11 A  158  ASP LEU LEU ASN PRO ILE GLY VAL THR GLY SER ASN PRO          
SEQRES  12 A  158  ASN LYS GLU THR PRO CYS LEU GLU LEU GLU PHE ASP TRP          
SEQRES  13 A  158  PHE SER                                                      
HELIX    1   1 PRO A   71  LEU A   73  5                                   3    
SHEET    1   A 4 GLU A  62  TYR A  69  0                                        
SHEET    2   A 4 ALA A  10  THR A  19 -1  N  ILE A  13   O  LEU A  64           
SHEET    3   A 4 CYS A 149  ASP A 155 -1  O  GLU A 151   N  LEU A  16           
SHEET    4   A 4 MET A 118  ASN A 121 -1  N  MET A 118   O  LEU A 152           
SHEET    1   B 3 GLU A  42  PRO A  43  0                                        
SHEET    2   B 3 LYS A  30  HIS A  39 -1  N  HIS A  39   O  GLU A  42           
SHEET    3   B 3 VAL A  48  ASN A  49 -1  O  VAL A  48   N  THR A  35           
SHEET    1   C 5 GLU A  42  PRO A  43  0                                        
SHEET    2   C 5 LYS A  30  HIS A  39 -1  N  HIS A  39   O  GLU A  42           
SHEET    3   C 5 ALA A  77  GLY A  88 -1  O  CYS A  80   N  GLY A  36           
SHEET    4   C 5 GLU A  94  ILE A 104 -1  O  GLY A 102   N  LEU A  81           
SHEET    5   C 5 TRP A 123  PRO A 124 -1  O  TRP A 123   N  TRP A 101           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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