GenomeNet

Database: PDB
Entry: 2FC9
LinkDB: 2FC9
Original site: 2FC9 
HEADER    RNA BINDING PROTEIN                     12-DEC-05   2FC9              
TITLE     SOLUTION STRUCTURE OF THE RRM_1 DOMAIN OF NCL PROTEIN                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: NCL PROTEIN;                                               
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: RRM_1 DOMAIN, RESIDUES 8-95;                               
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: CELL FREE SYNTHESIS;                              
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P050516-04                                
KEYWDS    NMR, STRUCTURE GENOMICS, RRM_1 DOMAIN, NCL PROTEIN,                   
KEYWDS   2 STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT ON PROTEIN             
KEYWDS   3 STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL                 
KEYWDS   4 GENOMICS/PROTEOMICS INITIATIVE, RSGI, RNA BINDING PROTEIN            
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    W.DANG,Y.MUTO,M.INOUE,T.KIGAWA,M.SHIROUZU,T.TERADA,                   
AUTHOR   2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE           
AUTHOR   3 (RSGI)                                                               
REVDAT   2   24-JUN-08 2FC9    1       SOURCE REMARK DBREF  SEQADV              
REVDAT   2 2                   1       VERSN                                    
REVDAT   1   12-JUN-06 2FC9    0                                                
JRNL        AUTH   W.DANG,Y.MUTO,M.INOUE,T.KIGAWA,M.SHIROUZU,T.TERADA,          
JRNL        AUTH 2 S.YOKOYAMA                                                   
JRNL        TITL   SOLUTION STRUCTURE OF THE RRM_1 DOMAIN OF NCL                
JRNL        TITL 2 PROTEIN                                                      
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT,P.                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2FC9 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 16-DEC-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB035707.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 20MM D-TRIS-HCL(PH7.0); 100MM      
REMARK 210                                   NACL; 1MM D-DTT; 0.02% NAN3;       
REMARK 210                                   90% H2O, 10% D2O                   
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 2.6, NMRPIPE               
REMARK 210                                   20031121, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.9321, CYANA 2.0.17        
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS,              
REMARK 210                                   STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY, TARGET FUNCTION            
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A  12       81.23    -65.49                                   
REMARK 500  1 ASN A  22       31.71     72.28                                   
REMARK 500  1 GLU A  29      -34.01    -38.77                                   
REMARK 500  1 ALA A  39      108.59    -54.26                                   
REMARK 500  1 SER A  72      -65.59    -96.89                                   
REMARK 500  1 CYS A  73      -30.31    -39.66                                   
REMARK 500  1 ASN A  74      126.07    -34.59                                   
REMARK 500  1 PRO A  94     -177.41    -69.77                                   
REMARK 500  1 SER A  96      153.06    -45.02                                   
REMARK 500  2 SER A   9      107.05    -51.08                                   
REMARK 500  2 ALA A  39      108.59    -54.12                                   
REMARK 500  2 LYS A  53      -31.69    -38.78                                   
REMARK 500  2 GLU A  64      -70.72    -73.97                                   
REMARK 500  2 SER A  72      -67.19    -96.42                                   
REMARK 500  2 ASN A  74      121.90    -34.61                                   
REMARK 500  3 ASN A   8      148.32   -170.15                                   
REMARK 500  3 THR A  10       83.35    -64.68                                   
REMARK 500  3 ASN A  22       48.32     74.87                                   
REMARK 500  3 ALA A  39      104.06    -52.73                                   
REMARK 500  3 SER A  72      -66.18    -96.03                                   
REMARK 500  3 CYS A  73      -32.05    -37.49                                   
REMARK 500  3 ASN A  74      125.97    -34.88                                   
REMARK 500  3 ARG A  91       73.11    -68.92                                   
REMARK 500  4 ASN A   8      103.73    -48.07                                   
REMARK 500  4 THR A  10      139.97    -34.73                                   
REMARK 500  4 ASN A  22       40.58     73.26                                   
REMARK 500  4 GLU A  29      -31.53    -38.38                                   
REMARK 500  4 ALA A  39      109.85    -55.81                                   
REMARK 500  4 GLU A  64      -70.73    -71.03                                   
REMARK 500  4 SER A  72      -67.05    -92.83                                   
REMARK 500  4 ASN A  74      125.57    -34.39                                   
REMARK 500  4 PRO A  90        2.57    -69.67                                   
REMARK 500  4 ASN A  95      105.86    -57.10                                   
REMARK 500  5 TRP A  11      115.41   -164.86                                   
REMARK 500  5 ASN A  22       47.54     74.26                                   
REMARK 500  5 ALA A  39      109.08    -50.18                                   
REMARK 500  5 SER A  62     -177.35   -175.48                                   
REMARK 500  5 SER A  72      -67.11    -96.68                                   
REMARK 500  5 CYS A  73      -30.67    -36.67                                   
REMARK 500  5 ASN A  74      124.10    -35.56                                   
REMARK 500  6 SER A   3       42.09    -93.53                                   
REMARK 500  6 ASN A  22       44.80     74.60                                   
REMARK 500  6 ALA A  39      105.10    -54.97                                   
REMARK 500  6 GLU A  64      -71.25    -72.00                                   
REMARK 500  6 SER A  72      -65.23    -92.81                                   
REMARK 500  6 ASN A  74      128.57    -34.40                                   
REMARK 500  6 ARG A  91      174.63    -55.02                                   
REMARK 500  6 ASN A  95      120.33    -34.54                                   
REMARK 500  7 SER A   2       99.89    -44.65                                   
REMARK 500  7 SER A   3       97.45    -45.07                                   
REMARK 500  7 ASN A   8      160.32    -42.01                                   
REMARK 500  7 TRP A  11      138.97   -171.94                                   
REMARK 500  7 ASN A  22       44.89     74.85                                   
REMARK 500  7 PHE A  41      148.14   -170.03                                   
REMARK 500  7 SER A  72      -63.79    -97.23                                   
REMARK 500  7 CYS A  73      -31.86    -38.44                                   
REMARK 500  7 ASN A  74      122.97    -35.34                                   
REMARK 500  8 SER A   5      -58.07   -129.80                                   
REMARK 500  8 THR A  10       39.16    -94.60                                   
REMARK 500  8 GLU A  14      115.27    -34.23                                   
REMARK 500  8 ASN A  22       42.39     73.68                                   
REMARK 500  8 SER A  62     -179.13   -175.23                                   
REMARK 500  8 SER A  72      -67.27    -94.03                                   
REMARK 500  8 ASN A  74      125.37    -34.84                                   
REMARK 500  9 SER A   6       41.74    -88.29                                   
REMARK 500  9 ASN A   8       38.17    -85.75                                   
REMARK 500  9 GLU A  14      114.75    -34.37                                   
REMARK 500  9 ASN A  22       41.50     74.23                                   
REMARK 500  9 ALA A  39      107.96    -55.16                                   
REMARK 500  9 LYS A  53      -33.31    -39.56                                   
REMARK 500  9 GLU A  64      -72.21    -64.20                                   
REMARK 500  9 SER A  72      -67.25    -92.77                                   
REMARK 500  9 ASN A  74      124.37    -34.32                                   
REMARK 500  9 PRO A  98       93.99    -69.77                                   
REMARK 500 10 TRP A  11      -74.42    -59.79                                   
REMARK 500 10 SER A  12       39.52     38.71                                   
REMARK 500 10 ASN A  22       46.13     74.88                                   
REMARK 500 10 GLU A  29      -29.32    -37.10                                   
REMARK 500 10 ALA A  39      107.90    -51.71                                   
REMARK 500 10 LYS A  53      -30.20    -38.95                                   
REMARK 500 10 GLU A  64      -72.77    -74.02                                   
REMARK 500 10 SER A  72      -65.98    -97.75                                   
REMARK 500 10 ASN A  74      121.79    -34.90                                   
REMARK 500 10 SER A  93      148.97   -174.51                                   
REMARK 500 11 SER A   5      117.51    -36.21                                   
REMARK 500 11 ASN A  22       47.04     70.53                                   
REMARK 500 11 ALA A  39      108.06    -49.93                                   
REMARK 500 11 SER A  62     -176.78   -175.63                                   
REMARK 500 11 SER A  72      -67.26    -93.27                                   
REMARK 500 11 ASN A  74      122.94    -34.01                                   
REMARK 500 11 PRO A  90     -172.51    -69.72                                   
REMARK 500 11 SER A  93      137.95    -35.69                                   
REMARK 500 12 SER A   2      141.12   -173.17                                   
REMARK 500 12 SER A   9      144.47    -39.65                                   
REMARK 500 12 ASN A  22       38.25     73.53                                   
REMARK 500 12 ALA A  39      108.60    -55.40                                   
REMARK 500 12 SER A  62     -178.24   -175.75                                   
REMARK 500 12 SER A  72      -67.22    -95.35                                   
REMARK 500 12 ASN A  74      114.60    -33.64                                   
REMARK 500 13 SER A  12       47.43    -90.32                                   
REMARK 500 13 ALA A  39      108.04    -53.97                                   
REMARK 500 13 GLU A  64      -73.24    -75.73                                   
REMARK 500 13 SER A  72      -66.20    -92.10                                   
REMARK 500 13 ASN A  74      122.23    -35.03                                   
REMARK 500 13 SER A  93       52.56     73.12                                   
REMARK 500 14 SER A   3      130.81   -171.92                                   
REMARK 500 14 SER A   5       42.17     34.80                                   
REMARK 500 14 ASN A  22       37.76     73.84                                   
REMARK 500 14 GLU A  29      -32.15    -38.92                                   
REMARK 500 14 GLU A  64      -73.76    -69.92                                   
REMARK 500 14 SER A  72      -65.75    -92.32                                   
REMARK 500 14 ASN A  74      122.08    -35.59                                   
REMARK 500 14 ASN A  95       92.30    -67.28                                   
REMARK 500 15 GLU A  14      107.60    -34.24                                   
REMARK 500 15 ASN A  22       35.89     74.99                                   
REMARK 500 15 ALA A  39      108.63    -50.96                                   
REMARK 500 15 PHE A  41      140.45   -170.02                                   
REMARK 500 15 SER A  72      -66.46    -96.75                                   
REMARK 500 15 CYS A  73      -30.67    -38.47                                   
REMARK 500 15 ASN A  74      121.56    -34.33                                   
REMARK 500 16 TRP A  11       33.61    -92.65                                   
REMARK 500 16 SER A  12       40.34     37.19                                   
REMARK 500 16 ASN A  22       38.74     74.22                                   
REMARK 500 16 ALA A  39      108.97    -54.39                                   
REMARK 500 16 GLU A  64      -72.28    -74.58                                   
REMARK 500 16 SER A  72      -65.66    -97.15                                   
REMARK 500 16 PRO A  90       89.22    -69.81                                   
REMARK 500 17 SER A   2      -55.09   -124.43                                   
REMARK 500 17 THR A  10     -177.46    -52.62                                   
REMARK 500 17 SER A  12       49.02     37.92                                   
REMARK 500 17 ASN A  22       42.19     75.01                                   
REMARK 500 17 LYS A  53      -32.01    -36.21                                   
REMARK 500 17 SER A  62     -176.16   -174.92                                   
REMARK 500 17 SER A  72      -65.62    -92.03                                   
REMARK 500 17 ASN A  74      120.48    -34.22                                   
REMARK 500 17 SER A  99      -47.20   -132.01                                   
REMARK 500 17 SER A 100      163.29    -41.81                                   
REMARK 500 18 TRP A  11      -68.79   -109.24                                   
REMARK 500 18 SER A  12       25.01     40.03                                   
REMARK 500 18 GLU A  29      -33.93    -39.54                                   
REMARK 500 18 ALA A  39      109.25    -56.69                                   
REMARK 500 18 SER A  62     -175.58   -175.11                                   
REMARK 500 18 SER A  72      -67.00    -90.59                                   
REMARK 500 18 ASN A  74      123.77    -34.27                                   
REMARK 500 18 ARG A  91       96.60    -60.17                                   
REMARK 500 18 PRO A  98       84.57    -69.72                                   
REMARK 500 19 SER A   2      102.58    -52.73                                   
REMARK 500 19 SER A   3      142.78   -173.26                                   
REMARK 500 19 SER A  12       80.13    -62.60                                   
REMARK 500 19 ASN A  22       48.49     74.94                                   
REMARK 500 19 SER A  72      -66.33    -97.76                                   
REMARK 500 19 ASN A  74      123.34    -37.89                                   
REMARK 500 19 PRO A  94       87.70    -69.80                                   
REMARK 500 19 PRO A  98       93.21    -69.76                                   
REMARK 500 20 SER A   5       42.33     36.38                                   
REMARK 500 20 ASN A  22       34.03     75.03                                   
REMARK 500 20 ALA A  39      104.48    -53.00                                   
REMARK 500 20 PHE A  41      135.95   -171.23                                   
REMARK 500 20 LYS A  53      -25.94    -39.58                                   
REMARK 500 20 SER A  62     -177.14   -174.91                                   
REMARK 500 20 SER A  72      -66.56    -93.24                                   
REMARK 500 20 ASN A  74      119.34    -35.66                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650 HELIX                                                                
REMARK 650 DETERMINATION METHOD: AUTHOR DETERMINED                              
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 2FC8   RELATED DB: PDB                                   
REMARK 900 THE DIFFERENT FRAGMENT OF THE SAME PROTEIN                           
REMARK 900 RELATED ID: HSI002005757.1   RELATED DB: TARGETDB                    
DBREF  2FC9 A    8    95  UNP    Q9BQ02   Q9BQ02_HUMAN   250    337             
SEQADV 2FC9 GLY A    1  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A    2  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A    3  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 GLY A    4  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A    5  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A    6  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 GLY A    7  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A   96  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 GLY A   97  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 PRO A   98  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A   99  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 SER A  100  UNP  Q9BQ02              EXPRESSION TAG                 
SEQADV 2FC9 GLY A  101  UNP  Q9BQ02              EXPRESSION TAG                 
SEQRES   1 A  101  GLY SER SER GLY SER SER GLY ASN SER THR TRP SER GLY          
SEQRES   2 A  101  GLU SER LYS THR LEU VAL LEU SER ASN LEU SER TYR SER          
SEQRES   3 A  101  ALA THR GLU GLU THR LEU GLN GLU VAL PHE GLU LYS ALA          
SEQRES   4 A  101  THR PHE ILE LYS VAL PRO GLN ASN GLN ASN GLY LYS SER          
SEQRES   5 A  101  LYS GLY TYR ALA PHE ILE GLU PHE ALA SER PHE GLU ASP          
SEQRES   6 A  101  ALA LYS GLU ALA LEU ASN SER CYS ASN LYS ARG GLU ILE          
SEQRES   7 A  101  GLU GLY ARG ALA ILE ARG LEU GLU LEU GLN GLY PRO ARG          
SEQRES   8 A  101  GLY SER PRO ASN SER GLY PRO SER SER GLY                      
HELIX    1   1 GLU A   29  GLN A   33  1                                   5    
HELIX    2   2 PHE A   63  CYS A   73  1                                  11    
SHEET    1   A 5 PHE A  41  LYS A  43  0                                        
SHEET    2   A 5 TYR A  55  GLU A  59 -1  O  GLU A  59   N  PHE A  41           
SHEET    3   A 5 THR A  17  SER A  21 -1  N  LEU A  20   O  ALA A  56           
SHEET    4   A 5 ARG A  81  LEU A  87 -1  O  GLU A  86   N  VAL A  19           
SHEET    5   A 5 ARG A  76  ILE A  78 -1  N  ILE A  78   O  ARG A  81           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system