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Database: PDB
Entry: 2FIO
LinkDB: 2FIO
Original site: 2FIO 
HEADER    TRANSCRIPTION/DNA                       30-DEC-05   2FIO              
TITLE     PHAGE PHI29 TRANSCRIPTION REGULATOR P4-DNA COMPLEX                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DNA (41-MER);                                              
COMPND   3 CHAIN: C;                                                            
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: DNA (41-MER);                                              
COMPND   7 CHAIN: D;                                                            
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 3;                                                           
COMPND  10 MOLECULE: LATE GENES ACTIVATOR;                                      
COMPND  11 CHAIN: A, B;                                                         
COMPND  12 SYNONYM: EARLY PROTEIN GP4;                                          
COMPND  13 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 MOL_ID: 2;                                                           
SOURCE   4 SYNTHETIC: YES;                                                      
SOURCE   5 MOL_ID: 3;                                                           
SOURCE   6 ORGANISM_SCIENTIFIC: BACILLUS PHAGE PHI29;                           
SOURCE   7 ORGANISM_TAXID: 10756;                                               
SOURCE   8 GENE: 4;                                                             
SOURCE   9 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  10 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  11 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID                               
KEYWDS    PROTEIN-DNA COMPLEX; N-HOOK DNA-BINDING MOTIF; TRANSCRIPTION          
KEYWDS   2 REGULATION, TRANSCRIPTION-DNA COMPLEX                                
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    D.BADIA,A.CAMACHO,L.PEREZ-LAGO,C.ESCANDON,M.SALAS,M.COLL              
REVDAT   4   18-OCT-17 2FIO    1       REMARK                                   
REVDAT   3   24-FEB-09 2FIO    1       VERSN                                    
REVDAT   2   03-OCT-06 2FIO    1       JRNL                                     
REVDAT   1   26-SEP-06 2FIO    0                                                
JRNL        AUTH   D.BADIA,A.CAMACHO,L.PEREZ-LAGO,C.ESCANDON,M.SALAS,M.COLL     
JRNL        TITL   THE STRUCTURE OF PHAGE PHI29 TRANSCRIPTION REGULATOR P4-DNA  
JRNL        TITL 2 COMPLEX REVEALS AN N-HOOK MOTIF FOR DNA                      
JRNL        REF    MOL.CELL                      V.  22    73 2006              
JRNL        REFN                   ISSN 1097-2765                               
JRNL        PMID   16600871                                                     
JRNL        DOI    10.1016/J.MOLCEL.2006.02.019                                 
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.70 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS                                                  
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,              
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.70                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 30.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL                           
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 99.1                           
REMARK   3   NUMBER OF REFLECTIONS             : 15751                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.229                           
REMARK   3   FREE R VALUE                     : 0.279                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 6.900                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1091                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL                         
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : NULL                         
REMARK   3   BIN FREE R VALUE                    : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL                         
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2016                                    
REMARK   3   NUCLEIC ACID ATOMS       : 1675                                    
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 86                                      
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 60.85                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -17.42000                                            
REMARK   3    B22 (A**2) : -2.37100                                             
REMARK   3    B33 (A**2) : 19.79100                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.27100                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL                            
REMARK   3   ESD FROM SIGMAA              (A) : NULL                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.007                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.260                           
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL                            
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : NULL                                      
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : NULL                                                 
REMARK   3   KSOL        : NULL                                                 
REMARK   3   BSOL        : 37.64                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : CNS_TOPPAR:PROTEIN_REP.PARAM                   
REMARK   3  PARAMETER FILE  2  : CNS_TOPPAR:DNA-RNA_REP.PARAM                   
REMARK   3  PARAMETER FILE  3  : CNS_TOPPAR:WATER_REP.PARAM                     
REMARK   3  PARAMETER FILE  4  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : NULL                                           
REMARK   3  TOPOLOGY FILE  2   : NULL                                           
REMARK   3  TOPOLOGY FILE  3   : NULL                                           
REMARK   3  TOPOLOGY FILE  4   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2FIO COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 06-FEB-06.                  
REMARK 100 THE DEPOSITION ID IS D_1000035923.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-NOV-04                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID13                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.978                              
REMARK 200  MONOCHROMATOR                  : SI 111                             
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH                        
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS                                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 13386                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.700                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 30.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 0.8                                
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.70                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.00                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 0.6                                
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: AMORE                                                 
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 50.93                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.51                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 20% PEG 6000, 80MM MAGNESIUM CHLORIDE,   
REMARK 280  50MM CHES, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE       
REMARK 280  277K                                                                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D, A, B                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     PRO B   115                                                      
REMARK 465     ARG B   116                                                      
REMARK 465     LEU B   117                                                      
REMARK 465     ARG B   118                                                      
REMARK 465     GLU B   119                                                      
REMARK 465     ARG B   120                                                      
REMARK 465     ARG B   121                                                      
REMARK 465     LYS B   122                                                      
REMARK 465     SER B   123                                                      
REMARK 465     MET B   124                                                      
REMARK 480                                                                      
REMARK 480 ZERO OCCUPANCY ATOM                                                  
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO                  
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS                
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;              
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):         
REMARK 480   M RES C SSEQI ATOMS                                                
REMARK 480     LYS A   36   CD   CE   NZ                                        
REMARK 480     LYS A   46   CD   CE   NZ                                        
REMARK 480     LYS A   50   CE   NZ                                             
REMARK 480     ARG A  118   CG   CD   NE   CZ   NH1  NH2                        
REMARK 480     GLU A  119   CG   CD   OE1  OE2                                  
REMARK 480     ARG A  121   CG   CD   NE   CZ   NH1  NH2                        
REMARK 480     LYS B    3   CE   NZ                                             
REMARK 480     LYS B   13   NZ                                                  
REMARK 480     LYS B   47   CD   CE   NZ                                        
REMARK 480     LYS B   50   CD   CE   NZ                                        
REMARK 480     GLU B   73   CG   CD   OE1  OE2                                  
REMARK 480     LYS B  114   CD   CE   NZ                                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   OP1   DC C    12     NH1  ARG A   118     1655     1.93            
REMARK 500   C2    DA C    41     C2    DA C    41     2656     2.00            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A   3      104.12     66.20                                   
REMARK 500    THR A  22      -22.16     88.19                                   
REMARK 500    PRO A  59       33.57    -83.14                                   
REMARK 500    PRO A 115      -78.68    -36.62                                   
REMARK 500    ARG A 116      125.12    125.33                                   
REMARK 500    LEU A 117      -42.64    -23.05                                   
REMARK 500    THR B   4     -179.32    -66.43                                   
REMARK 500    ASN B  11       77.41   -109.25                                   
REMARK 500    THR B  22      -23.23     77.82                                   
REMARK 500    ILE B 100       -9.99    -51.45                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  2FIO A    2   124  UNP    P03682   VG4_BPPH2        2    124             
DBREF  2FIO B    2   124  UNP    P03682   VG4_BPPH2        2    124             
DBREF  2FIO C    1    41  PDB    2FIO     2FIO             1     41             
DBREF  2FIO D    1    41  PDB    2FIO     2FIO             1     41             
SEQRES   1 C   41   DA  DA  DA  DA  DA  DC  DG  DT  DC  DA  DA  DC  DA          
SEQRES   2 C   41   DT  DT  DT  DT  DA  DT  DA  DA  DA  DA  DA  DA  DG          
SEQRES   3 C   41   DT  DC  DT  DT  DG  DC  DA  DA  DA  DA  DA  DG  DT          
SEQRES   4 C   41   DT  DA                                                      
SEQRES   1 D   41   DT  DA  DA  DC  DT  DT  DT  DT  DT  DG  DC  DA  DA          
SEQRES   2 D   41   DG  DA  DC  DT  DT  DT  DT  DT  DT  DA  DT  DA  DA          
SEQRES   3 D   41   DA  DA  DT  DG  DT  DT  DG  DA  DC  DG  DT  DT  DT          
SEQRES   4 D   41   DT  DT                                                      
SEQRES   1 A  123  PRO LYS THR GLN ARG GLY ILE TYR HIS ASN LEU LYS GLU          
SEQRES   2 A  123  SER GLU TYR VAL ALA SER ASN THR ASP VAL THR PHE PHE          
SEQRES   3 A  123  PHE SER SER GLU LEU TYR LEU ASN LYS PHE LEU ASP GLY          
SEQRES   4 A  123  TYR GLN GLU TYR ARG LYS LYS PHE ASN LYS LYS ILE GLU          
SEQRES   5 A  123  ARG VAL ALA VAL THR PRO TRP ASN MET ASP MET LEU ALA          
SEQRES   6 A  123  ASP ILE THR PHE TYR SER GLU VAL GLU LYS ARG GLY PHE          
SEQRES   7 A  123  HIS ALA TRP LEU LYS GLY ASP ASN ALA THR TRP ARG GLU          
SEQRES   8 A  123  VAL HIS VAL TYR ALA LEU ARG ILE MET THR LYS PRO ASN          
SEQRES   9 A  123  THR LEU ASP TRP SER ARG ILE GLN LYS PRO ARG LEU ARG          
SEQRES  10 A  123  GLU ARG ARG LYS SER MET                                      
SEQRES   1 B  123  PRO LYS THR GLN ARG GLY ILE TYR HIS ASN LEU LYS GLU          
SEQRES   2 B  123  SER GLU TYR VAL ALA SER ASN THR ASP VAL THR PHE PHE          
SEQRES   3 B  123  PHE SER SER GLU LEU TYR LEU ASN LYS PHE LEU ASP GLY          
SEQRES   4 B  123  TYR GLN GLU TYR ARG LYS LYS PHE ASN LYS LYS ILE GLU          
SEQRES   5 B  123  ARG VAL ALA VAL THR PRO TRP ASN MET ASP MET LEU ALA          
SEQRES   6 B  123  ASP ILE THR PHE TYR SER GLU VAL GLU LYS ARG GLY PHE          
SEQRES   7 B  123  HIS ALA TRP LEU LYS GLY ASP ASN ALA THR TRP ARG GLU          
SEQRES   8 B  123  VAL HIS VAL TYR ALA LEU ARG ILE MET THR LYS PRO ASN          
SEQRES   9 B  123  THR LEU ASP TRP SER ARG ILE GLN LYS PRO ARG LEU ARG          
SEQRES  10 B  123  GLU ARG ARG LYS SER MET                                      
FORMUL   5  HOH   *86(H2 O)                                                     
HELIX    1   1 ASN A   11  SER A   15  5                                   5    
HELIX    2   2 SER A   30  ARG A   54  1                                  25    
HELIX    3   3 MET A   62  GLU A   75  1                                  14    
HELIX    4   4 THR A   89  MET A  101  1                                  13    
HELIX    5   5 ARG A  116  MET A  124  1                                   9    
HELIX    6   6 ASN B   11  SER B   15  5                                   5    
HELIX    7   7 SER B   30  ALA B   56  1                                  27    
HELIX    8   8 MET B   62  GLU B   75  1                                  14    
HELIX    9   9 THR B   89  ILE B  100  1                                  12    
HELIX   10  10 MET B  101  LYS B  103  5                                   3    
SHEET    1   A 5 ASP A  86  ALA A  88  0                                        
SHEET    2   A 5 ALA A  81  LEU A  83 -1  N  ALA A  81   O  ALA A  88           
SHEET    3   A 5 VAL A  24  PHE A  28 -1  N  THR A  25   O  TRP A  82           
SHEET    4   A 5 TYR A  17  SER A  20 -1  N  ALA A  19   O  PHE A  26           
SHEET    5   A 5 SER A 110  ARG A 111 -1  O  SER A 110   N  SER A  20           
SHEET    1   B 5 ASP B  86  ASN B  87  0                                        
SHEET    2   B 5 ALA B  81  LEU B  83 -1  N  LEU B  83   O  ASP B  86           
SHEET    3   B 5 VAL B  24  PHE B  27 -1  N  THR B  25   O  TRP B  82           
SHEET    4   B 5 VAL B  18  SER B  20 -1  N  ALA B  19   O  PHE B  26           
SHEET    5   B 5 SER B 110  ILE B 112 -1  O  ILE B 112   N  VAL B  18           
CRYST1   52.400   27.800  192.800  90.00  97.40  90.00 P 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.019084  0.000000  0.002479        0.00000                         
SCALE2      0.000000  0.035971  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.005230        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system