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Database: PDB
Entry: 2GPQ
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HEADER    TRANSLATION                             18-APR-06   2GPQ              
TITLE     CAP-FREE STRUCTURE OF EIF4E SUGGESTS BASIS FOR ITS                    
TITLE    2 ALLOSTERIC REGULATION                                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E;               
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: EIF4E, EIF-4E, MRNA CAP-BINDING PROTEIN, EIF-4F             
COMPND   5 25 KDA SUBUNIT                                                       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606                                                 
KEYWDS    TRANSLATION REGULATION, EIF4E, APO FORM                               
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    L.VOLPON,M.J.OSBORNE,K.L.B.BORDEN                                     
REVDAT   2   24-FEB-09 2GPQ    1       VERSN                                    
REVDAT   1   14-NOV-06 2GPQ    0                                                
JRNL        AUTH   L.VOLPON,M.J.OSBORNE,I.TOPISIROVIC,N.SIDDIQUI,               
JRNL        AUTH 2 K.L.BORDEN                                                   
JRNL        TITL   CAP-FREE STRUCTURE OF EIF4E SUGGESTS A BASIS FOR             
JRNL        TITL 2 CONFORMATIONAL REGULATION BY ITS LIGANDS.                    
JRNL        REF    EMBO J.                       V.  25  5138 2006              
JRNL        REFN                   ISSN 0261-4189                               
JRNL        PMID   17036047                                                     
JRNL        DOI    10.1038/SJ.EMBOJ.7601380                                     
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON A TOTAL       
REMARK   3  OF 1973 RESTRAINTS, 1697 ARE NOE-DERIVED DISTANCE CONSTRAINTS,      
REMARK   3  276 DIHEDRAL ANGLE RESTRAINTS, 160 DISTANCE RESTRAINTS FROM         
REMARK   3  HYDROGEN BONDS                                                      
REMARK   4                                                                      
REMARK   4 2GPQ COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 19-APR-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB037403.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 293                                
REMARK 210  PH                             : 7.4                                
REMARK 210  IONIC STRENGTH                 : 100 MM NACL                        
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.3MM EIF4E U-98% 15N, U-13C;      
REMARK 210                                   50MM PHOSPHATE BUFFER; 100MM       
REMARK 210                                   NACL; 1MM DTT; 0.02% NAN3; 90%     
REMARK 210                                   H2O, 10% D2O; 0.3MM EIF4E U-       
REMARK 210                                   98% 15N, U-95% D; 50MM             
REMARK 210                                   PHOSPHATE BUFFER; 100MM NACL;      
REMARK 210                                   1MM DTT; 0.02% NAN3; 90% H2O,      
REMARK 210                                   10% D2O                            
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : HNCA, HNCO, HCCH-TOCSY, 3D         
REMARK 210                                   15N-EDITED NOESY, 3D-13C-          
REMARK 210                                   EDITED NOESY                       
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1C, NMRPIPE 2.3,            
REMARK 210                                   SPARKY 3.112                       
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 50                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS,              
REMARK 210                                   STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR      
REMARK 210  SPECTROSCOPY                                                        
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ALA A   2      131.32   -175.11                                   
REMARK 500  1 GLU A   7      129.80   -175.28                                   
REMARK 500  1 THR A  16      -43.21   -164.14                                   
REMARK 500  1 GLU A  20       79.80     61.91                                   
REMARK 500  1 GLU A  23      161.11     60.62                                   
REMARK 500  1 SER A  24      -74.37   -153.60                                   
REMARK 500  1 ASN A  25      109.15   -176.75                                   
REMARK 500  1 GLN A  26       30.92   -149.88                                   
REMARK 500  1 TYR A  34       64.43    172.58                                   
REMARK 500  1 LYS A  52       67.39    -67.03                                   
REMARK 500  1 SER A  53      -35.26    172.94                                   
REMARK 500  1 SER A  64      171.37    179.49                                   
REMARK 500  1 LEU A  81       37.62    163.96                                   
REMARK 500  1 ASN A  84       33.10   -145.24                                   
REMARK 500  1 LEU A  85       39.60    -97.77                                   
REMARK 500  1 PRO A 100       65.54    -67.82                                   
REMARK 500  1 TRP A 102      -68.05   -127.35                                   
REMARK 500  1 GLU A 140      141.00    169.33                                   
REMARK 500  1 SER A 141      -65.07     65.56                                   
REMARK 500  1 ASP A 143     -162.24     49.35                                   
REMARK 500  1 ALA A 158      -85.34     58.24                                   
REMARK 500  1 LYS A 192      -68.03   -178.39                                   
REMARK 500  1 ALA A 204       30.02    -97.95                                   
REMARK 500  1 THR A 205      -68.91   -162.32                                   
REMARK 500  1 LYS A 206     -169.20     45.84                                   
REMARK 500  1 SER A 207      105.92     60.28                                   
REMARK 500  1 THR A 210      -74.54   -121.18                                   
REMARK 500  1 THR A 211     -168.47   -111.48                                   
REMARK 500  1 LYS A 212       77.59    -66.26                                   
REMARK 500  1 ASN A 213       89.59   -177.32                                   
REMARK 500  1 ARG A 214       50.65    127.79                                   
REMARK 500  2 GLU A  20       88.46    -69.49                                   
REMARK 500  2 THR A  22      168.14     57.30                                   
REMARK 500  2 ASN A  25       81.16   -177.31                                   
REMARK 500  2 VAL A  28      144.28   -179.81                                   
REMARK 500  2 ALA A  29      -90.27    -85.90                                   
REMARK 500  2 ASN A  30      -57.96    163.83                                   
REMARK 500  2 GLU A  32      115.71   -167.84                                   
REMARK 500  2 HIS A  33      -46.93   -178.59                                   
REMARK 500  2 TYR A  34      134.60   -180.00                                   
REMARK 500  2 SER A  53      -33.24    179.37                                   
REMARK 500  2 SER A  64      166.71    179.62                                   
REMARK 500  2 ILE A  79       82.12   -157.73                                   
REMARK 500  2 LEU A  81     -165.35     44.88                                   
REMARK 500  2 SER A  82      -65.40     70.37                                   
REMARK 500  2 GLU A 140       37.02   -177.09                                   
REMARK 500  2 SER A 141      -58.53   -178.12                                   
REMARK 500  2 ASP A 143     -163.34     68.08                                   
REMARK 500  2 TYR A 145       46.64    -87.39                                   
REMARK 500  2 ALA A 158      -87.14     55.64                                   
REMARK 500  2 LYS A 192      -43.06   -168.89                                   
REMARK 500  2 LYS A 206      -78.22    -91.06                                   
REMARK 500  2 SER A 207      -81.49     62.23                                   
REMARK 500  2 THR A 210      138.53     63.39                                   
REMARK 500  2 THR A 211       66.13   -177.97                                   
REMARK 500  2 ASN A 213       22.99   -154.50                                   
REMARK 500  2 ARG A 214       41.06    177.99                                   
REMARK 500  3 GLU A   7     -176.55     55.24                                   
REMARK 500  3 PRO A  12      109.88    -53.31                                   
REMARK 500  3 ASN A  13      158.23    178.88                                   
REMARK 500  3 GLU A  20     -172.67    -60.12                                   
REMARK 500  3 GLU A  27       91.58     60.02                                   
REMARK 500  3 ALA A  29       80.77     59.60                                   
REMARK 500  3 ASN A  30      -61.74   -177.66                                   
REMARK 500  3 HIS A  33       32.09   -164.71                                   
REMARK 500  3 TYR A  34       84.46   -158.04                                   
REMARK 500  3 SER A  64      162.50    179.36                                   
REMARK 500  3 GLN A  80       99.09    -57.04                                   
REMARK 500  3 LEU A  81       -9.58     94.44                                   
REMARK 500  3 ASN A  84       79.53   -152.72                                   
REMARK 500  3 TRP A 102      -68.26   -127.00                                   
REMARK 500  3 GLU A 140     -102.96   -141.35                                   
REMARK 500  3 SER A 141      -76.66     58.20                                   
REMARK 500  3 ASP A 143     -162.48     64.26                                   
REMARK 500  3 LYS A 192      -73.80   -159.36                                   
REMARK 500  3 LYS A 206      162.37     59.20                                   
REMARK 500  3 THR A 210       94.56    -58.83                                   
REMARK 500  3 LYS A 212       72.05   -177.65                                   
REMARK 500  3 ARG A 214       55.25    126.52                                   
REMARK 500  4 ALA A   2      111.99     60.80                                   
REMARK 500  4 GLU A   7      -67.92    -97.86                                   
REMARK 500  4 ASN A  13       48.80   -171.13                                   
REMARK 500  4 THR A  16      -52.16   -163.60                                   
REMARK 500  4 THR A  17     -166.80     44.57                                   
REMARK 500  4 GLU A  18     -165.03   -119.95                                   
REMARK 500  4 GLU A  19     -162.76     66.73                                   
REMARK 500  4 GLU A  20      -60.89     76.02                                   
REMARK 500  4 GLU A  23       77.52   -170.70                                   
REMARK 500  4 SER A  24     -169.25   -120.93                                   
REMARK 500  4 GLN A  26      -72.45     66.98                                   
REMARK 500  4 ALA A  29      -76.63   -137.45                                   
REMARK 500  4 ASN A  30      -62.24   -177.19                                   
REMARK 500  4 GLU A  32      -76.50   -129.06                                   
REMARK 500  4 HIS A  33      -54.36   -164.65                                   
REMARK 500  4 TYR A  34       98.16     77.03                                   
REMARK 500  4 SER A  53      -34.43    178.79                                   
REMARK 500  4 ILE A  79      -43.98   -140.75                                   
REMARK 500  4 LEU A  81       46.72    168.68                                   
REMARK 500  4 ASN A  84       69.21   -150.04                                   
REMARK 500  4 PRO A 100       73.02    -66.09                                   
REMARK 500  4 MET A 101       75.10     63.05                                   
REMARK 500  4 TRP A 102      -68.49   -141.40                                   
REMARK 500  4 GLU A 140       28.21   -161.11                                   
REMARK 500  4 SER A 141      -77.86   -129.27                                   
REMARK 500  4 ASP A 143      -82.38     43.18                                   
REMARK 500  4 ASP A 144      -45.13    -27.22                                   
REMARK 500  4 ALA A 158      -85.10     59.24                                   
REMARK 500  4 LEU A 189       87.69    -24.69                                   
REMARK 500  4 PRO A 191      -75.59    -73.06                                   
REMARK 500  4 LYS A 192       46.48    178.06                                   
REMARK 500  4 LYS A 206       86.17    -60.88                                   
REMARK 500  4 SER A 207      137.96   -175.83                                   
REMARK 500  4 THR A 210     -168.89    -69.34                                   
REMARK 500  4 THR A 211      167.09     57.54                                   
REMARK 500  4 ARG A 214       45.66    165.12                                   
REMARK 500  5 ALA A   2      -73.96    -94.50                                   
REMARK 500  5 GLU A   7       93.06   -166.45                                   
REMARK 500  5 THR A  16      -79.03   -137.84                                   
REMARK 500  5 THR A  22     -169.94     56.84                                   
REMARK 500  5 GLU A  23      -68.11   -123.75                                   
REMARK 500  5 SER A  24     -170.82   -176.42                                   
REMARK 500  5 PRO A  31       63.83    -65.91                                   
REMARK 500  5 GLU A  32       36.91   -178.16                                   
REMARK 500  5 HIS A  33       41.84    -94.54                                   
REMARK 500  5 TYR A  34       52.92   -171.67                                   
REMARK 500  5 SER A  53       67.32   -151.77                                   
REMARK 500  5 SER A  64      179.01    179.63                                   
REMARK 500  5 LEU A  85       70.13   -103.14                                   
REMARK 500  5 TRP A 102      -68.11   -120.53                                   
REMARK 500  5 GLU A 140      -92.92   -156.93                                   
REMARK 500  5 SER A 141      -68.70     58.01                                   
REMARK 500  5 PHE A 142       34.77    -89.25                                   
REMARK 500  5 ASP A 143     -163.93     39.23                                   
REMARK 500  5 SER A 146       64.98    -67.94                                   
REMARK 500  5 VAL A 149       85.93    -58.71                                   
REMARK 500  5 ALA A 158      -88.12     54.18                                   
REMARK 500  5 PRO A 191       41.33    -92.90                                   
REMARK 500  5 LYS A 192      -51.31    179.95                                   
REMARK 500  5 ALA A 204     -173.87     50.36                                   
REMARK 500  5 SER A 207       78.59   -116.87                                   
REMARK 500  5 SER A 209      179.46     59.64                                   
REMARK 500  5 THR A 210      110.62   -163.71                                   
REMARK 500  5 THR A 211      -43.93   -162.08                                   
REMARK 500  5 ASN A 213       67.09   -118.71                                   
REMARK 500  5 ARG A 214       39.49    166.49                                   
REMARK 500  6 PRO A   6      -73.64    -59.79                                   
REMARK 500  6 GLU A   7      -63.77   -177.49                                   
REMARK 500  6 ASN A  13       98.45   -173.97                                   
REMARK 500  6 GLU A  18      -70.10   -176.63                                   
REMARK 500  6 SER A  24      112.03     60.91                                   
REMARK 500  6 ASN A  25      -68.05   -143.52                                   
REMARK 500  6 GLN A  26      -75.87   -101.88                                   
REMARK 500  6 GLU A  27      115.61   -176.39                                   
REMARK 500  6 ASN A  30      167.17     58.43                                   
REMARK 500  6 HIS A  33      117.17     62.52                                   
REMARK 500  6 LYS A  52      105.31    -57.98                                   
REMARK 500  6 SER A  53       67.20     62.67                                   
REMARK 500  6 SER A  64      164.99    179.77                                   
REMARK 500  6 ILE A  98      147.77    -38.91                                   
REMARK 500  6 PRO A 100       43.36    -82.25                                   
REMARK 500  6 MET A 101       72.79     57.16                                   
REMARK 500  6 TRP A 102      -44.89   -141.50                                   
REMARK 500  6 GLU A 103      -41.08   -136.01                                   
REMARK 500  6 GLU A 140       78.88   -172.23                                   
REMARK 500  6 SER A 141      -63.06   -101.71                                   
REMARK 500  6 PHE A 142       38.42    -79.51                                   
REMARK 500  6 ASP A 143     -162.64     45.79                                   
REMARK 500  6 TYR A 145       38.99    -90.38                                   
REMARK 500  6 VAL A 149      108.17    -53.79                                   
REMARK 500  6 LEU A 189       89.79    -28.76                                   
REMARK 500  6 PRO A 191      -76.10    -82.58                                   
REMARK 500  6 LYS A 192       55.26   -176.30                                   
REMARK 500  6 SER A 207     -169.32   -161.47                                   
REMARK 500  6 THR A 210      -53.68   -140.68                                   
REMARK 500  6 THR A 211       47.56    179.38                                   
REMARK 500  6 ARG A 214       55.30    125.99                                   
REMARK 500  7 ALA A   2      -75.36   -170.27                                   
REMARK 500  7 GLU A   7       30.99    -98.62                                   
REMARK 500  7 ASN A  13      -61.57   -178.41                                   
REMARK 500  7 GLU A  20      -69.74   -157.78                                   
REMARK 500  7 GLU A  23      112.21     61.21                                   
REMARK 500  7 ASN A  25      103.64     61.44                                   
REMARK 500  7 GLU A  27     -174.08     60.42                                   
REMARK 500  7 VAL A  28      164.81     66.17                                   
REMARK 500  7 ALA A  29       59.47     76.07                                   
REMARK 500  7 ASN A  30      -59.27   -178.66                                   
REMARK 500  7 PRO A  31       47.20    -84.73                                   
REMARK 500  7 GLU A  32      114.41     61.42                                   
REMARK 500  7 TYR A  34      104.18   -178.63                                   
REMARK 500  7 ASN A  59     -170.41     62.25                                   
REMARK 500  7 ILE A  79      107.25     59.01                                   
REMARK 500  7 GLN A  80       89.28    -62.05                                   
REMARK 500  7 LEU A  81     -154.69     39.87                                   
REMARK 500  7 MET A 101       65.74     60.69                                   
REMARK 500  7 TRP A 102      -68.11   -141.05                                   
REMARK 500  7 GLU A 140      -64.62   -155.19                                   
REMARK 500  7 SER A 141      -69.02     66.89                                   
REMARK 500  7 ASP A 143      -77.60     49.93                                   
REMARK 500  7 ASP A 144      -45.67    -25.17                                   
REMARK 500  7 TYR A 145       65.37   -166.41                                   
REMARK 500  7 SER A 146       38.90    -96.16                                   
REMARK 500  7 LEU A 189       49.39   -153.77                                   
REMARK 500  7 THR A 205      -82.46    -59.15                                   
REMARK 500  7 LYS A 206       77.30    177.00                                   
REMARK 500  7 SER A 207     -175.33     58.68                                   
REMARK 500  7 SER A 209       31.03    -98.70                                   
REMARK 500  7 THR A 211       76.24   -110.74                                   
REMARK 500  7 ARG A 214       41.43    167.65                                   
REMARK 500  8 ASN A  13       71.43   -116.03                                   
REMARK 500  8 THR A  17      171.91     51.00                                   
REMARK 500  8 GLU A  19       58.88   -100.58                                   
REMARK 500  8 GLU A  20      -72.01    -57.16                                   
REMARK 500  8 LYS A  21       75.78     58.43                                   
REMARK 500  8 GLU A  23       83.14     59.14                                   
REMARK 500  8 SER A  24      -77.49   -176.89                                   
REMARK 500  8 ASN A  25      149.99     62.05                                   
REMARK 500  8 GLN A  26      -68.51     68.54                                   
REMARK 500  8 ASN A  30      -58.51    177.74                                   
REMARK 500  8 GLU A  32       95.75     80.76                                   
REMARK 500  8 HIS A  33      119.52     64.67                                   
REMARK 500  8 LYS A  52      -68.11     67.56                                   
REMARK 500  8 SER A  53       87.28   -171.53                                   
REMARK 500  8 SER A  64      177.74    179.97                                   
REMARK 500  8 GLN A  80      106.30    -57.81                                   
REMARK 500  8 LEU A  81       94.01    -41.04                                   
REMARK 500  8 SER A  82      -48.77    173.15                                   
REMARK 500  8 PRO A 100       80.43    -58.36                                   
REMARK 500  8 MET A 101       71.91     46.65                                   
REMARK 500  8 TRP A 102      -68.10   -141.10                                   
REMARK 500  8 SER A 141      -59.65   -177.50                                   
REMARK 500  8 ASP A 143     -162.81     63.27                                   
REMARK 500  8 TYR A 145       35.33    -84.52                                   
REMARK 500  8 ASN A 172       69.69    -69.51                                   
REMARK 500  8 LEU A 189     -173.69    -42.00                                   
REMARK 500  8 PRO A 190      -51.48    -29.02                                   
REMARK 500  8 PRO A 191       -7.01    -51.34                                   
REMARK 500  8 LYS A 192      -45.86   -143.82                                   
REMARK 500  8 THR A 205       35.75    -96.60                                   
REMARK 500  8 LYS A 206      -73.91     64.62                                   
REMARK 500  8 SER A 207       79.09   -175.77                                   
REMARK 500  8 SER A 209       92.38    -59.45                                   
REMARK 500  8 THR A 210      -44.17   -163.88                                   
REMARK 500  8 LYS A 212       95.90     37.71                                   
REMARK 500  8 ASN A 213       28.58   -140.15                                   
REMARK 500  8 ARG A 214       37.95    179.07                                   
REMARK 500  9 GLU A   7     -178.18     60.87                                   
REMARK 500  9 ASN A  13      -63.62   -154.84                                   
REMARK 500  9 THR A  16       86.66    -59.59                                   
REMARK 500  9 THR A  17      -73.50     66.19                                   
REMARK 500  9 GLU A  20       79.84    -68.04                                   
REMARK 500  9 LYS A  21       31.43    -98.47                                   
REMARK 500  9 ASN A  25      -48.81   -137.82                                   
REMARK 500  9 GLN A  26       53.57   -103.86                                   
REMARK 500  9 VAL A  28       85.82     73.19                                   
REMARK 500  9 ALA A  29       29.85   -162.04                                   
REMARK 500  9 ASN A  30      -68.23   -143.53                                   
REMARK 500  9 TYR A  34       57.92     82.71                                   
REMARK 500  9 ILE A  79       28.56   -147.18                                   
REMARK 500  9 LEU A  81     -152.18    156.25                                   
REMARK 500  9 ASN A  84       83.12    179.72                                   
REMARK 500  9 LEU A  85       39.51    178.25                                   
REMARK 500  9 TRP A 102      -68.59   -141.03                                   
REMARK 500  9 GLU A 140       52.04    167.18                                   
REMARK 500  9 SER A 141      -65.90   -168.11                                   
REMARK 500  9 PHE A 142       35.50    -94.46                                   
REMARK 500  9 ASP A 143     -163.98     70.95                                   
REMARK 500  9 TYR A 145      -35.00    -35.39                                   
REMARK 500  9 SER A 146       84.43    -52.84                                   
REMARK 500  9 ASP A 147      -40.81   -156.73                                   
REMARK 500  9 VAL A 149       92.98    -65.38                                   
REMARK 500  9 ALA A 158      -85.72     55.68                                   
REMARK 500  9 ASN A 172       67.76    -69.31                                   
REMARK 500  9 PRO A 191       38.52    -86.54                                   
REMARK 500  9 LYS A 192      -75.04   -177.66                                   
REMARK 500  9 ALA A 204       44.60    -94.79                                   
REMARK 500  9 THR A 205      -50.86   -176.03                                   
REMARK 500  9 LYS A 212      -68.89   -173.72                                   
REMARK 500  9 ARG A 214       41.34    171.55                                   
REMARK 500 10 ALA A   2      -79.18     64.19                                   
REMARK 500 10 PRO A   6      -75.86    -60.05                                   
REMARK 500 10 GLU A   7     -168.96    177.40                                   
REMARK 500 10 PRO A  12      109.88    -56.20                                   
REMARK 500 10 ASN A  13      -64.84   -176.80                                   
REMARK 500 10 THR A  16     -166.07     65.40                                   
REMARK 500 10 THR A  17      -74.87     65.70                                   
REMARK 500 10 GLU A  18      -73.42   -177.73                                   
REMARK 500 10 GLU A  19      -76.23     64.13                                   
REMARK 500 10 GLU A  20       63.86   -169.47                                   
REMARK 500 10 SER A  24      105.42   -176.84                                   
REMARK 500 10 ALA A  29       86.77     74.59                                   
REMARK 500 10 ASN A  30      -60.19   -178.61                                   
REMARK 500 10 GLU A  32      -85.04     63.98                                   
REMARK 500 10 HIS A  33      -21.45    156.38                                   
REMARK 500 10 SER A  64      163.24    179.96                                   
REMARK 500 10 GLN A  80       96.05    -62.90                                   
REMARK 500 10 LEU A  81      -47.27    173.77                                   
REMARK 500 10 LEU A  85       74.59     73.64                                   
REMARK 500 10 PRO A 100       62.19    -66.93                                   
REMARK 500 10 MET A 101       68.08     69.68                                   
REMARK 500 10 TRP A 102      -68.02   -142.14                                   
REMARK 500 10 GLU A 140      -41.20   -177.92                                   
REMARK 500 10 SER A 141      121.81     56.45                                   
REMARK 500 10 ASP A 143      -73.12     75.54                                   
REMARK 500 10 ASP A 144      -18.28    -39.79                                   
REMARK 500 10 SER A 146       91.35    -68.32                                   
REMARK 500 10 ASP A 147       41.33    -93.38                                   
REMARK 500 10 VAL A 149       90.64    -54.27                                   
REMARK 500 10 ALA A 158      -82.85     28.86                                   
REMARK 500 10 LEU A 189       93.65    -34.02                                   
REMARK 500 10 PRO A 191      -72.61    -85.94                                   
REMARK 500 10 LYS A 192       41.61    179.78                                   
REMARK 500 10 ALA A 204     -167.05    -64.42                                   
REMARK 500 10 SER A 207     -169.34   -108.82                                   
REMARK 500 10 SER A 209     -174.07   -176.73                                   
REMARK 500 10 THR A 211       81.40     59.14                                   
REMARK 500 10 ARG A 214       53.36    126.96                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  2GPQ A    1   217  UNP    P06730   IF4E_HUMAN       1    217             
SEQRES   1 A  217  MET ALA THR VAL GLU PRO GLU THR THR PRO THR PRO ASN          
SEQRES   2 A  217  PRO PRO THR THR GLU GLU GLU LYS THR GLU SER ASN GLN          
SEQRES   3 A  217  GLU VAL ALA ASN PRO GLU HIS TYR ILE LYS HIS PRO LEU          
SEQRES   4 A  217  GLN ASN ARG TRP ALA LEU TRP PHE PHE LYS ASN ASP LYS          
SEQRES   5 A  217  SER LYS THR TRP GLN ALA ASN LEU ARG LEU ILE SER LYS          
SEQRES   6 A  217  PHE ASP THR VAL GLU ASP PHE TRP ALA LEU TYR ASN HIS          
SEQRES   7 A  217  ILE GLN LEU SER SER ASN LEU MET PRO GLY CYS ASP TYR          
SEQRES   8 A  217  SER LEU PHE LYS ASP GLY ILE GLU PRO MET TRP GLU ASP          
SEQRES   9 A  217  GLU LYS ASN LYS ARG GLY GLY ARG TRP LEU ILE THR LEU          
SEQRES  10 A  217  ASN LYS GLN GLN ARG ARG SER ASP LEU ASP ARG PHE TRP          
SEQRES  11 A  217  LEU GLU THR LEU LEU CYS LEU ILE GLY GLU SER PHE ASP          
SEQRES  12 A  217  ASP TYR SER ASP ASP VAL CYS GLY ALA VAL VAL ASN VAL          
SEQRES  13 A  217  ARG ALA LYS GLY ASP LYS ILE ALA ILE TRP THR THR GLU          
SEQRES  14 A  217  CYS GLU ASN ARG GLU ALA VAL THR HIS ILE GLY ARG VAL          
SEQRES  15 A  217  TYR LYS GLU ARG LEU GLY LEU PRO PRO LYS ILE VAL ILE          
SEQRES  16 A  217  GLY TYR GLN SER HIS ALA ASP THR ALA THR LYS SER GLY          
SEQRES  17 A  217  SER THR THR LYS ASN ARG PHE VAL VAL                          
HELIX    1   1 VAL A   69  GLN A   80  1                                  12    
HELIX    2   2 GLU A  105  GLY A  110  1                                   6    
HELIX    3   3 GLN A  120  ARG A  122  5                                   3    
HELIX    4   4 SER A  124  GLU A  140  1                                  17    
HELIX    5   5 PHE A  142  ASP A  147  5                                   6    
HELIX    6   6 ASN A  172  GLY A  188  1                                  17    
HELIX    7   7 ALA A  201  THR A  205  5                                   5    
SHEET    1   A 7 LEU A  60  THR A  68  0                                        
SHEET    2   A 7 PRO A  38  LYS A  49 -1  N  LEU A  45   O  SER A  64           
SHEET    3   A 7 CYS A  89  LYS A  95 -1  O  SER A  92   N  TRP A  46           
SHEET    4   A 7 VAL A 149  ARG A 157 -1  O  VAL A 154   N  TYR A  91           
SHEET    5   A 7 GLY A 160  THR A 167 -1  O  TRP A 166   N  GLY A 151           
SHEET    6   A 7 GLY A 111  ASN A 118 -1  N  ILE A 115   O  ILE A 163           
SHEET    7   A 7 GLY A 196  SER A 199 -1  O  GLY A 196   N  LEU A 114           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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