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Database: PDB
Entry: 2KJY
LinkDB: 2KJY
Original site: 2KJY 
HEADER    SIGNALING PROTEIN                       11-JUN-09   2KJY              
TITLE     MYPT1(658-714)                                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12A;              
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 658- 714;                                     
COMPND   5 SYNONYM: MYOSIN PHOSPHATASE-TARGETING SUBUNIT 1, MYOSIN              
COMPND   6 PHOSPHATASE TARGET SUBUNIT 1, PROTEIN PHOSPHATASE MYOSIN-            
COMPND   7 BINDING SUBUNIT;                                                     
COMPND   8 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: PPP1R12A, MBS, MYPT1;                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PET                                       
KEYWDS    MYPT1, PHOSPHORYLATION, PROTEIN PHOSPHATASE 1, MYOSIN                 
KEYWDS   2 PHOSPHATASE, ALTERNATIVE SPLICING, ANK REPEAT, CYTOPLASM,            
KEYWDS   3 PHOSPHOPROTEIN, POLYMORPHISM, SIGNALING PROTEIN                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    S.MORI,R.IWAOKA,M.ETO,S.OHKI                                          
REVDAT   2   29-SEP-09 2KJY    1       JRNL                                     
REVDAT   1   08-SEP-09 2KJY    0                                                
JRNL        AUTH   S.MORI,R.IWAOKA,M.ETO,S.Y.OHKI                               
JRNL        TITL   SOLUTION STRUCTURE OF THE INHIBITORY                         
JRNL        TITL 2 PHOSPHORYLATION DOMAIN OF MYOSIN PHOSPHATASE                 
JRNL        TITL 3 TARGETING SUBUNIT 1                                          
JRNL        REF    PROTEINS                      V.  77   732 2009              
JRNL        REFN                   ISSN 0887-3585                               
JRNL        PMID   19701943                                                     
JRNL        DOI    10.1002/PROT.22529                                           
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR_NIH 2.1                                       
REMARK   3   AUTHORS     : SCHWIETERS, KUSZEWSKI, TJANDRA, CLORE                
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2KJY COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 15-JUN-09.                  
REMARK 100 THE RCSB ID CODE IS RCSB101213.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 285                                
REMARK 210  PH                             : 6.0                                
REMARK 210  IONIC STRENGTH                 : KCL @100                           
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.6-1.0MM MYPT1(658-714)-1,        
REMARK 210                                   0.6-1.0MM [U-99% 15N]              
REMARK 210                                   MYPT1(658-714)-2, 0.6-1.0MM [U     
REMARK 210                                   -99% 13C; U-99% 15N]               
REMARK 210                                   MYPT1(658-714)-3, 90% H2O/10%      
REMARK 210                                   D2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC; 3D CBCA(CO)        
REMARK 210                                   NH; 3D HNCACB; 3D C(CO)NH; 3D      
REMARK 210                                   H(CCO)NH; 3D HCCH-TOCSY; 3D 1H     
REMARK 210                                   -15N NOESY; 2D 1H-1H NOESY; 3D     
REMARK 210                                   1H-15N TOCSY                       
REMARK 210  SPECTROMETER FIELD STRENGTH    : 750 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : X-PLOR_NIH 2.1                     
REMARK 210   METHOD USED                   : DGSA-DISTANCE GEOMETRY             
REMARK 210                                   SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 LEU A  16       38.00   -143.57                                   
REMARK 500  1 GLN A  43     -178.47    178.13                                   
REMARK 500  1 VAL A  45      -12.30     70.26                                   
REMARK 500  1 LEU A  47      -53.17   -140.18                                   
REMARK 500  1 ILE A  57      160.63     57.95                                   
REMARK 500  1 ARG A  59     -160.29   -116.79                                   
REMARK 500  2 SER A   4       45.22   -161.97                                   
REMARK 500  2 GLN A  43      -77.37     62.94                                   
REMARK 500  2 VAL A  45      -44.12   -130.31                                   
REMARK 500  2 ILE A  57       79.73     52.36                                   
REMARK 500  3 SER A   4       42.52   -153.80                                   
REMARK 500  3 TYR A  15      -30.75    -38.73                                   
REMARK 500  3 LEU A  16       42.69   -141.33                                   
REMARK 500  3 VAL A  45      -10.97     69.59                                   
REMARK 500  3 LEU A  47      -58.97   -140.94                                   
REMARK 500  3 ARG A  59      -57.25   -141.34                                   
REMARK 500  4 MET A   3     -158.96    -70.94                                   
REMARK 500  4 LEU A  16       30.09   -150.40                                   
REMARK 500  4 GLN A  43     -157.46     61.57                                   
REMARK 500  4 VAL A  45      -12.08     71.27                                   
REMARK 500  4 LEU A  47      -52.21   -141.50                                   
REMARK 500  4 ARG A  59      -88.11     54.44                                   
REMARK 500  5 GLN A  43     -178.42    178.14                                   
REMARK 500  5 VAL A  45      -11.42     69.49                                   
REMARK 500  5 THR A  46       38.95     36.49                                   
REMARK 500  5 LEU A  47      -43.95   -139.99                                   
REMARK 500  6 MET A   3     -177.92     53.07                                   
REMARK 500  6 LEU A  16       40.24   -143.25                                   
REMARK 500  6 GLN A  43     -160.61    170.65                                   
REMARK 500  6 VAL A  45      -50.54   -150.47                                   
REMARK 500  6 ILE A  57       94.70     52.92                                   
REMARK 500  6 ARG A  59      -73.09    -62.32                                   
REMARK 500  7 SER A   4       35.73   -155.25                                   
REMARK 500  7 GLN A  43     -139.39     61.70                                   
REMARK 500  7 ARG A  59     -162.64     54.31                                   
REMARK 500  8 MET A   3     -176.53     52.29                                   
REMARK 500  8 SER A   4       39.26   -149.33                                   
REMARK 500  8 TYR A  15      -31.55    -39.51                                   
REMARK 500  8 GLN A  43     -162.14     59.30                                   
REMARK 500  8 VAL A  45      -12.22     71.18                                   
REMARK 500  8 THR A  46       29.39     45.47                                   
REMARK 500  8 LEU A  47      -60.92   -143.15                                   
REMARK 500  8 ARG A  59       87.08     52.53                                   
REMARK 500  9 LEU A  16       43.18   -148.08                                   
REMARK 500  9 GLN A  43     -163.33     55.14                                   
REMARK 500  9 VAL A  45      -12.61     70.67                                   
REMARK 500  9 LEU A  47      -45.58   -132.37                                   
REMARK 500  9 ILE A  57       48.04   -109.84                                   
REMARK 500  9 ARG A  59       52.03   -167.82                                   
REMARK 500 10 MET A   3       75.14     52.18                                   
REMARK 500 10 LEU A  16       37.01   -160.35                                   
REMARK 500 10 GLN A  43     -159.60    178.40                                   
REMARK 500 10 VAL A  45      -13.38     71.09                                   
REMARK 500 11 LEU A  16       39.05   -159.80                                   
REMARK 500 11 GLN A  43     -160.13     59.58                                   
REMARK 500 11 VAL A  45      -10.10     68.43                                   
REMARK 500 11 LEU A  47      -63.68   -133.68                                   
REMARK 500 11 ILE A  57       94.28     52.66                                   
REMARK 500 12 SER A   4       72.60     47.52                                   
REMARK 500 12 TYR A  15      -30.19    -38.83                                   
REMARK 500 12 GLN A  43       19.41     54.41                                   
REMARK 500 12 LEU A  47      -43.59   -145.82                                   
REMARK 500 13 MET A   3     -163.04   -119.27                                   
REMARK 500 13 LEU A  16       39.04   -144.12                                   
REMARK 500 13 VAL A  45      -10.32     69.39                                   
REMARK 500 14 SER A   4      -63.70     66.93                                   
REMARK 500 14 LEU A  16       39.02   -142.47                                   
REMARK 500 14 GLN A  43      -30.26    176.63                                   
REMARK 500 14 THR A  46       71.38    -61.94                                   
REMARK 500 14 LEU A  47      -44.92   -154.73                                   
REMARK 500 14 ARG A  59       33.56   -156.84                                   
REMARK 500 15 MET A   3       52.00   -146.31                                   
REMARK 500 15 GLN A  43      -80.09     61.74                                   
REMARK 500 15 VAL A  45      -11.55     68.70                                   
REMARK 500 15 THR A  46       27.73     47.99                                   
REMARK 500 15 LEU A  47      -52.66   -140.42                                   
REMARK 500 15 ILE A  57      114.32   -166.06                                   
REMARK 500 15 ARG A  59       40.71   -143.82                                   
REMARK 500 16 LEU A  16       38.85   -144.44                                   
REMARK 500 16 GLN A  43      -39.12    177.59                                   
REMARK 500 16 VAL A  45      -10.10     69.57                                   
REMARK 500 16 THR A  46       47.83     36.29                                   
REMARK 500 16 LEU A  47      -40.67   -154.81                                   
REMARK 500 16 ILE A  57       92.26     52.56                                   
REMARK 500 16 ARG A  59       43.28   -162.15                                   
REMARK 500 17 LEU A  16       40.11   -141.33                                   
REMARK 500 17 VAL A  45      -11.87     71.57                                   
REMARK 500 17 LEU A  47      -58.50   -136.38                                   
REMARK 500 17 ARG A  59      174.90     55.53                                   
REMARK 500 18 MET A   3      -87.70     54.83                                   
REMARK 500 18 LEU A  16       38.43   -143.63                                   
REMARK 500 18 GLN A  43      176.92    179.84                                   
REMARK 500 18 VAL A  45      -47.72   -138.58                                   
REMARK 500 18 ILE A  57       37.60    -99.69                                   
REMARK 500 19 MET A   3       73.65     52.92                                   
REMARK 500 19 SER A   4       35.53    -97.06                                   
REMARK 500 19 LEU A  16       40.20   -141.46                                   
REMARK 500 19 GLN A  43     -157.30   -175.41                                   
REMARK 500 19 VAL A  45      -11.37     69.32                                   
REMARK 500 19 THR A  46       27.25     48.76                                   
REMARK 500 19 ILE A  57       88.34     53.16                                   
REMARK 500 20 TYR A  15      -30.77    -38.42                                   
REMARK 500 20 LEU A  16       46.55   -149.29                                   
REMARK 500 20 GLN A  43      178.98     56.43                                   
REMARK 500 20 VAL A  45     -123.96     57.65                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A   9         0.22    SIDE_CHAIN                              
REMARK 500  1 ARG A  11         0.32    SIDE_CHAIN                              
REMARK 500  1 ARG A  12         0.28    SIDE_CHAIN                              
REMARK 500  1 ARG A  13         0.21    SIDE_CHAIN                              
REMARK 500  1 ARG A  20         0.23    SIDE_CHAIN                              
REMARK 500  1 ARG A  28         0.23    SIDE_CHAIN                              
REMARK 500  1 ARG A  31         0.32    SIDE_CHAIN                              
REMARK 500  1 ARG A  33         0.32    SIDE_CHAIN                              
REMARK 500  1 ARG A  36         0.23    SIDE_CHAIN                              
REMARK 500  1 ARG A  39         0.32    SIDE_CHAIN                              
REMARK 500  1 ARG A  40         0.25    SIDE_CHAIN                              
REMARK 500  1 ARG A  59         0.32    SIDE_CHAIN                              
REMARK 500  2 ARG A   9         0.29    SIDE_CHAIN                              
REMARK 500  2 ARG A  11         0.23    SIDE_CHAIN                              
REMARK 500  2 ARG A  12         0.27    SIDE_CHAIN                              
REMARK 500  2 ARG A  13         0.27    SIDE_CHAIN                              
REMARK 500  2 ARG A  20         0.30    SIDE_CHAIN                              
REMARK 500  2 ARG A  28         0.24    SIDE_CHAIN                              
REMARK 500  2 ARG A  31         0.22    SIDE_CHAIN                              
REMARK 500  2 ARG A  33         0.25    SIDE_CHAIN                              
REMARK 500  2 ARG A  36         0.26    SIDE_CHAIN                              
REMARK 500  2 ARG A  39         0.24    SIDE_CHAIN                              
REMARK 500  2 ARG A  40         0.29    SIDE_CHAIN                              
REMARK 500  2 ARG A  59         0.22    SIDE_CHAIN                              
REMARK 500  3 ARG A   9         0.23    SIDE_CHAIN                              
REMARK 500  3 ARG A  11         0.26    SIDE_CHAIN                              
REMARK 500  3 ARG A  12         0.31    SIDE_CHAIN                              
REMARK 500  3 ARG A  13         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  20         0.30    SIDE_CHAIN                              
REMARK 500  3 ARG A  28         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  31         0.29    SIDE_CHAIN                              
REMARK 500  3 ARG A  33         0.30    SIDE_CHAIN                              
REMARK 500  3 ARG A  36         0.31    SIDE_CHAIN                              
REMARK 500  3 ARG A  39         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  40         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  59         0.32    SIDE_CHAIN                              
REMARK 500  4 ARG A   9         0.32    SIDE_CHAIN                              
REMARK 500  4 ARG A  11         0.32    SIDE_CHAIN                              
REMARK 500  4 ARG A  12         0.23    SIDE_CHAIN                              
REMARK 500  4 ARG A  13         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG A  20         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG A  28         0.29    SIDE_CHAIN                              
REMARK 500  4 ARG A  31         0.21    SIDE_CHAIN                              
REMARK 500  4 ARG A  33         0.24    SIDE_CHAIN                              
REMARK 500  4 ARG A  36         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG A  39         0.31    SIDE_CHAIN                              
REMARK 500  4 ARG A  40         0.26    SIDE_CHAIN                              
REMARK 500  4 ARG A  59         0.28    SIDE_CHAIN                              
REMARK 500  5 ARG A   9         0.23    SIDE_CHAIN                              
REMARK 500  5 ARG A  11         0.25    SIDE_CHAIN                              
REMARK 500  5 ARG A  12         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG A  13         0.29    SIDE_CHAIN                              
REMARK 500  5 ARG A  20         0.30    SIDE_CHAIN                              
REMARK 500  5 ARG A  28         0.22    SIDE_CHAIN                              
REMARK 500  5 ARG A  31         0.23    SIDE_CHAIN                              
REMARK 500  5 ARG A  33         0.29    SIDE_CHAIN                              
REMARK 500  5 ARG A  36         0.27    SIDE_CHAIN                              
REMARK 500  5 ARG A  39         0.21    SIDE_CHAIN                              
REMARK 500  5 ARG A  40         0.30    SIDE_CHAIN                              
REMARK 500  5 ARG A  59         0.23    SIDE_CHAIN                              
REMARK 500  6 ARG A   9         0.23    SIDE_CHAIN                              
REMARK 500  6 ARG A  11         0.27    SIDE_CHAIN                              
REMARK 500  6 ARG A  12         0.26    SIDE_CHAIN                              
REMARK 500  6 ARG A  13         0.22    SIDE_CHAIN                              
REMARK 500  6 ARG A  20         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG A  28         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG A  31         0.21    SIDE_CHAIN                              
REMARK 500  6 ARG A  33         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG A  36         0.23    SIDE_CHAIN                              
REMARK 500  6 ARG A  39         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG A  40         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG A  59         0.23    SIDE_CHAIN                              
REMARK 500  7 ARG A   9         0.27    SIDE_CHAIN                              
REMARK 500  7 ARG A  11         0.28    SIDE_CHAIN                              
REMARK 500  7 ARG A  12         0.25    SIDE_CHAIN                              
REMARK 500  7 ARG A  13         0.30    SIDE_CHAIN                              
REMARK 500  7 ARG A  20         0.28    SIDE_CHAIN                              
REMARK 500  7 ARG A  28         0.32    SIDE_CHAIN                              
REMARK 500  7 ARG A  31         0.25    SIDE_CHAIN                              
REMARK 500  7 ARG A  33         0.30    SIDE_CHAIN                              
REMARK 500  7 ARG A  36         0.29    SIDE_CHAIN                              
REMARK 500  7 ARG A  39         0.23    SIDE_CHAIN                              
REMARK 500  7 ARG A  40         0.30    SIDE_CHAIN                              
REMARK 500  7 ARG A  59         0.23    SIDE_CHAIN                              
REMARK 500  8 ARG A   9         0.20    SIDE_CHAIN                              
REMARK 500  8 ARG A  11         0.23    SIDE_CHAIN                              
REMARK 500  8 ARG A  12         0.28    SIDE_CHAIN                              
REMARK 500  8 ARG A  13         0.25    SIDE_CHAIN                              
REMARK 500  8 ARG A  20         0.23    SIDE_CHAIN                              
REMARK 500  8 ARG A  28         0.21    SIDE_CHAIN                              
REMARK 500  8 ARG A  31         0.23    SIDE_CHAIN                              
REMARK 500  8 ARG A  33         0.23    SIDE_CHAIN                              
REMARK 500  8 ARG A  36         0.23    SIDE_CHAIN                              
REMARK 500  8 ARG A  39         0.32    SIDE_CHAIN                              
REMARK 500  8 ARG A  40         0.30    SIDE_CHAIN                              
REMARK 500  8 ARG A  59         0.31    SIDE_CHAIN                              
REMARK 500  9 ARG A   9         0.25    SIDE_CHAIN                              
REMARK 500  9 ARG A  11         0.21    SIDE_CHAIN                              
REMARK 500  9 ARG A  12         0.21    SIDE_CHAIN                              
REMARK 500  9 ARG A  13         0.25    SIDE_CHAIN                              
REMARK 500  9 ARG A  20         0.21    SIDE_CHAIN                              
REMARK 500  9 ARG A  28         0.31    SIDE_CHAIN                              
REMARK 500  9 ARG A  31         0.25    SIDE_CHAIN                              
REMARK 500  9 ARG A  33         0.26    SIDE_CHAIN                              
REMARK 500  9 ARG A  36         0.21    SIDE_CHAIN                              
REMARK 500  9 ARG A  39         0.26    SIDE_CHAIN                              
REMARK 500  9 ARG A  40         0.24    SIDE_CHAIN                              
REMARK 500  9 ARG A  59         0.24    SIDE_CHAIN                              
REMARK 500 10 ARG A   9         0.31    SIDE_CHAIN                              
REMARK 500 10 ARG A  11         0.23    SIDE_CHAIN                              
REMARK 500 10 ARG A  12         0.24    SIDE_CHAIN                              
REMARK 500 10 ARG A  13         0.22    SIDE_CHAIN                              
REMARK 500 10 ARG A  20         0.21    SIDE_CHAIN                              
REMARK 500 10 ARG A  28         0.25    SIDE_CHAIN                              
REMARK 500 10 ARG A  31         0.24    SIDE_CHAIN                              
REMARK 500 10 ARG A  33         0.32    SIDE_CHAIN                              
REMARK 500 10 ARG A  36         0.23    SIDE_CHAIN                              
REMARK 500 10 ARG A  39         0.26    SIDE_CHAIN                              
REMARK 500 10 ARG A  40         0.29    SIDE_CHAIN                              
REMARK 500 10 ARG A  59         0.28    SIDE_CHAIN                              
REMARK 500 11 ARG A   9         0.22    SIDE_CHAIN                              
REMARK 500 11 ARG A  11         0.31    SIDE_CHAIN                              
REMARK 500 11 ARG A  12         0.28    SIDE_CHAIN                              
REMARK 500 11 ARG A  13         0.30    SIDE_CHAIN                              
REMARK 500 11 ARG A  20         0.22    SIDE_CHAIN                              
REMARK 500 11 ARG A  28         0.32    SIDE_CHAIN                              
REMARK 500 11 ARG A  31         0.26    SIDE_CHAIN                              
REMARK 500 11 ARG A  33         0.22    SIDE_CHAIN                              
REMARK 500 11 ARG A  36         0.32    SIDE_CHAIN                              
REMARK 500 11 ARG A  39         0.31    SIDE_CHAIN                              
REMARK 500 11 ARG A  40         0.32    SIDE_CHAIN                              
REMARK 500 11 ARG A  59         0.32    SIDE_CHAIN                              
REMARK 500 12 ARG A   9         0.27    SIDE_CHAIN                              
REMARK 500 12 ARG A  11         0.21    SIDE_CHAIN                              
REMARK 500 12 ARG A  12         0.21    SIDE_CHAIN                              
REMARK 500 12 ARG A  13         0.25    SIDE_CHAIN                              
REMARK 500 12 ARG A  20         0.25    SIDE_CHAIN                              
REMARK 500 12 ARG A  28         0.25    SIDE_CHAIN                              
REMARK 500 12 ARG A  31         0.32    SIDE_CHAIN                              
REMARK 500 12 ARG A  33         0.28    SIDE_CHAIN                              
REMARK 500 12 ARG A  36         0.32    SIDE_CHAIN                              
REMARK 500 12 ARG A  39         0.21    SIDE_CHAIN                              
REMARK 500 12 ARG A  40         0.26    SIDE_CHAIN                              
REMARK 500 12 ARG A  59         0.31    SIDE_CHAIN                              
REMARK 500 13 ARG A   9         0.32    SIDE_CHAIN                              
REMARK 500 13 ARG A  11         0.25    SIDE_CHAIN                              
REMARK 500 13 ARG A  12         0.31    SIDE_CHAIN                              
REMARK 500 13 ARG A  13         0.22    SIDE_CHAIN                              
REMARK 500 13 ARG A  20         0.31    SIDE_CHAIN                              
REMARK 500 13 ARG A  28         0.26    SIDE_CHAIN                              
REMARK 500 13 ARG A  31         0.28    SIDE_CHAIN                              
REMARK 500 13 ARG A  33         0.31    SIDE_CHAIN                              
REMARK 500 13 ARG A  36         0.31    SIDE_CHAIN                              
REMARK 500 13 ARG A  39         0.30    SIDE_CHAIN                              
REMARK 500 13 ARG A  40         0.23    SIDE_CHAIN                              
REMARK 500 13 ARG A  59         0.26    SIDE_CHAIN                              
REMARK 500 14 ARG A   9         0.30    SIDE_CHAIN                              
REMARK 500 14 ARG A  11         0.30    SIDE_CHAIN                              
REMARK 500 14 ARG A  12         0.22    SIDE_CHAIN                              
REMARK 500 14 ARG A  13         0.28    SIDE_CHAIN                              
REMARK 500 14 ARG A  20         0.26    SIDE_CHAIN                              
REMARK 500 14 ARG A  28         0.31    SIDE_CHAIN                              
REMARK 500 14 ARG A  31         0.29    SIDE_CHAIN                              
REMARK 500 14 ARG A  33         0.24    SIDE_CHAIN                              
REMARK 500 14 ARG A  36         0.26    SIDE_CHAIN                              
REMARK 500 14 ARG A  39         0.24    SIDE_CHAIN                              
REMARK 500 14 ARG A  40         0.21    SIDE_CHAIN                              
REMARK 500 14 ARG A  59         0.27    SIDE_CHAIN                              
REMARK 500 15 ARG A   9         0.25    SIDE_CHAIN                              
REMARK 500 15 ARG A  11         0.29    SIDE_CHAIN                              
REMARK 500 15 ARG A  12         0.24    SIDE_CHAIN                              
REMARK 500 15 ARG A  13         0.27    SIDE_CHAIN                              
REMARK 500 15 ARG A  20         0.22    SIDE_CHAIN                              
REMARK 500 15 ARG A  28         0.30    SIDE_CHAIN                              
REMARK 500 15 ARG A  31         0.30    SIDE_CHAIN                              
REMARK 500 15 ARG A  33         0.24    SIDE_CHAIN                              
REMARK 500 15 ARG A  36         0.31    SIDE_CHAIN                              
REMARK 500 15 ARG A  39         0.29    SIDE_CHAIN                              
REMARK 500 15 ARG A  40         0.32    SIDE_CHAIN                              
REMARK 500 15 ARG A  59         0.25    SIDE_CHAIN                              
REMARK 500 16 ARG A   9         0.29    SIDE_CHAIN                              
REMARK 500 16 ARG A  11         0.29    SIDE_CHAIN                              
REMARK 500 16 ARG A  12         0.27    SIDE_CHAIN                              
REMARK 500 16 ARG A  13         0.30    SIDE_CHAIN                              
REMARK 500 16 ARG A  20         0.22    SIDE_CHAIN                              
REMARK 500 16 ARG A  28         0.23    SIDE_CHAIN                              
REMARK 500 16 ARG A  31         0.21    SIDE_CHAIN                              
REMARK 500 16 ARG A  33         0.24    SIDE_CHAIN                              
REMARK 500 16 ARG A  36         0.28    SIDE_CHAIN                              
REMARK 500 16 ARG A  39         0.30    SIDE_CHAIN                              
REMARK 500 16 ARG A  40         0.22    SIDE_CHAIN                              
REMARK 500 16 ARG A  59         0.25    SIDE_CHAIN                              
REMARK 500 17 ARG A   9         0.32    SIDE_CHAIN                              
REMARK 500 17 ARG A  11         0.22    SIDE_CHAIN                              
REMARK 500 17 ARG A  12         0.30    SIDE_CHAIN                              
REMARK 500 17 ARG A  13         0.23    SIDE_CHAIN                              
REMARK 500 17 ARG A  20         0.31    SIDE_CHAIN                              
REMARK 500 17 ARG A  28         0.32    SIDE_CHAIN                              
REMARK 500 17 ARG A  31         0.25    SIDE_CHAIN                              
REMARK 500 17 ARG A  33         0.30    SIDE_CHAIN                              
REMARK 500 17 ARG A  36         0.27    SIDE_CHAIN                              
REMARK 500 17 ARG A  39         0.27    SIDE_CHAIN                              
REMARK 500 17 ARG A  40         0.23    SIDE_CHAIN                              
REMARK 500 17 ARG A  59         0.24    SIDE_CHAIN                              
REMARK 500 18 ARG A   9         0.29    SIDE_CHAIN                              
REMARK 500 18 ARG A  11         0.30    SIDE_CHAIN                              
REMARK 500 18 ARG A  12         0.23    SIDE_CHAIN                              
REMARK 500 18 ARG A  13         0.20    SIDE_CHAIN                              
REMARK 500 18 ARG A  20         0.28    SIDE_CHAIN                              
REMARK 500 18 ARG A  28         0.26    SIDE_CHAIN                              
REMARK 500 18 ARG A  31         0.22    SIDE_CHAIN                              
REMARK 500 18 ARG A  33         0.27    SIDE_CHAIN                              
REMARK 500 18 ARG A  36         0.26    SIDE_CHAIN                              
REMARK 500 18 ARG A  39         0.29    SIDE_CHAIN                              
REMARK 500 18 ARG A  40         0.27    SIDE_CHAIN                              
REMARK 500 18 ARG A  59         0.26    SIDE_CHAIN                              
REMARK 500 19 ARG A   9         0.28    SIDE_CHAIN                              
REMARK 500 19 ARG A  11         0.26    SIDE_CHAIN                              
REMARK 500 19 ARG A  12         0.28    SIDE_CHAIN                              
REMARK 500 19 ARG A  13         0.31    SIDE_CHAIN                              
REMARK 500 19 ARG A  20         0.28    SIDE_CHAIN                              
REMARK 500 19 ARG A  28         0.27    SIDE_CHAIN                              
REMARK 500 19 ARG A  31         0.27    SIDE_CHAIN                              
REMARK 500 19 ARG A  33         0.23    SIDE_CHAIN                              
REMARK 500 19 ARG A  36         0.28    SIDE_CHAIN                              
REMARK 500 19 ARG A  39         0.28    SIDE_CHAIN                              
REMARK 500 19 ARG A  40         0.29    SIDE_CHAIN                              
REMARK 500 19 ARG A  59         0.29    SIDE_CHAIN                              
REMARK 500 20 ARG A   9         0.24    SIDE_CHAIN                              
REMARK 500 20 ARG A  11         0.26    SIDE_CHAIN                              
REMARK 500 20 ARG A  12         0.29    SIDE_CHAIN                              
REMARK 500 20 ARG A  13         0.24    SIDE_CHAIN                              
REMARK 500 20 ARG A  20         0.25    SIDE_CHAIN                              
REMARK 500 20 ARG A  28         0.32    SIDE_CHAIN                              
REMARK 500 20 ARG A  31         0.31    SIDE_CHAIN                              
REMARK 500 20 ARG A  33         0.32    SIDE_CHAIN                              
REMARK 500 20 ARG A  36         0.23    SIDE_CHAIN                              
REMARK 500 20 ARG A  39         0.24    SIDE_CHAIN                              
REMARK 500 20 ARG A  40         0.23    SIDE_CHAIN                              
REMARK 500 20 ARG A  59         0.22    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  2KJY A    4    60  UNP    O14974   MYPT1_HUMAN    658    714             
SEQADV 2KJY GLY A    1  UNP  O14974              EXPRESSION TAG                 
SEQADV 2KJY PRO A    2  UNP  O14974              EXPRESSION TAG                 
SEQADV 2KJY MET A    3  UNP  O14974              EXPRESSION TAG                 
SEQRES   1 A   60  GLY PRO MET SER THR THR GLU VAL ARG GLU ARG ARG ARG          
SEQRES   2 A   60  SER TYR LEU THR PRO VAL ARG ASP GLU GLU SER GLU SER          
SEQRES   3 A   60  GLN ARG LYS ALA ARG SER ARG GLN ALA ARG GLN SER ARG          
SEQRES   4 A   60  ARG SER THR GLN GLY VAL THR LEU THR ASP LEU GLN GLU          
SEQRES   5 A   60  ALA GLU LYS THR ILE GLY ARG SER                              
HELIX    1   1 SER A    4  TYR A   15  1                                  12    
HELIX    2   2 LEU A   16  PRO A   18  5                                   3    
HELIX    3   3 VAL A   19  GLN A   43  1                                  25    
HELIX    4   4 LEU A   47  ILE A   57  1                                  11    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system