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Database: PDB
Entry: 2L34
LinkDB: 2L34
Original site: 2L34 
HEADER    PROTEIN BINDING                         06-SEP-10   2L34              
TITLE     STRUCTURE OF THE DAP12 TRANSMEMBRANE HOMODIMER                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TYRO PROTEIN TYROSINE KINASE-BINDING PROTEIN;              
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 35-67;                                        
COMPND   5 SYNONYM: DNAX-ACTIVATION PROTEIN 12, KILLER-ACTIVATING RECEPTOR-     
COMPND   6 ASSOCIATED PROTEIN, KAR-ASSOCIATED PROTEIN;                          
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TYROBP, DAP12, KARAP;                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: VECTOR;                               
SOURCE   9 EXPRESSION_SYSTEM_VECTOR: TRPLE                                      
KEYWDS    IMMUNORECEPTOR, TRANSMEMBRANE ASSEMBLY, DAP12, PROTEIN BINDING        
EXPDTA    SOLUTION NMR                                                          
NUMMDL    15                                                                    
AUTHOR    M.E.CALL,K.W.WUCHERPFENNIG,J.J.CHOU                                   
REVDAT   1   03-NOV-10 2L34    0                                                
JRNL        AUTH   M.E.CALL,K.W.WUCHERPFENNIG,J.J.CHOU                          
JRNL        TITL   THE STRUCTURAL BASIS FOR INTRAMEMBRANE ASSEMBLY OF AN        
JRNL        TITL 2 ACTIVATING IMMUNORECEPTOR COMPLEX.                           
JRNL        REF    NAT.IMMUNOL.                  V.  11  1023 2010              
JRNL        REFN                   ISSN 1529-2908                               
JRNL        PMID   20890284                                                     
JRNL        DOI    10.1038/NI.1943                                              
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR_NIH                                           
REMARK   3   AUTHORS     : SCHWIETERS, KUSZEWSKI, TJANDRA AND CLORE             
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2L34 COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 17-SEP-10.                  
REMARK 100 THE RCSB ID CODE IS RCSB101896.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 303                                
REMARK 210  PH                             : 6.8                                
REMARK 210  IONIC STRENGTH                 : 20                                 
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.5 (DIMER) MM [U-100% 13C; U-     
REMARK 210                                   100% 15N; U-80% 2H] DAP12          
REMARK 210                                   TRANSMEMBRANE PEPTIDE-1, 20 MM     
REMARK 210                                   SODIUM PHOSPHATE-2, 25 MM SDS-3,   
REMARK 210                                   250 MM FOSCHOLINE-14-4, 95% H2O/   
REMARK 210                                   5% D2O; 0.5 (DIMER) MM [U-100%     
REMARK 210                                   13C; U-100% 15N] DAP12             
REMARK 210                                   TRANSMEMBRANE PEPTIDE-5, 20 MM     
REMARK 210                                   SODIUM PHOSPHATE-6, 25 MM [U-100%  
REMARK 210                                   2H] SDS-7, 250 MM [U-100% 2H]      
REMARK 210                                   FOSCHOLINE-14-8, 95% H2O/5% D2O;   
REMARK 210                                   0.5 (MONOMER) MM [U-100% 15N; U-   
REMARK 210                                   100% 2H] DAP12 TRANSMEMBRANE       
REMARK 210                                   PEPTIDE-9, 0.5 (MONOMER) MM [U-    
REMARK 210                                   100% 13C] DAP12 TRANSMEMBRANE      
REMARK 210                                   PEPTIDE-10, 25 MM [U-100% 2H] SDS  
REMARK 210                                   -11, 250 MM [U-100% 2H]            
REMARK 210                                   FOSCHOLINE-14-12, 20 MM SODIUM     
REMARK 210                                   PHOSPHATE-13, 95% H2O/5% D2O; 0.5  
REMARK 210                                   (DIMER) MM [U-10% 13C] DAP12       
REMARK 210                                   TRANSMEMBRANE PEPTIDE-14, 25 MM    
REMARK 210                                   [U-100% 2H] SDS-15, 250 MM [U-     
REMARK 210                                   100% 2H] FOSCHOLINE-14-16, 20 MM   
REMARK 210                                   SODIUM PHOSPHATE-17, 95% H2O/5%    
REMARK 210                                   D2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC; 2D 1H-13C HSQC;    
REMARK 210                                   3D HNCA; 3D HN(CO)CA; 3D HNCACB;   
REMARK 210                                   3D HN(COCA)CB; 3D 1H-15N NOESY;    
REMARK 210                                   3D 1H-13C NOESY                    
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NMRPIPE, XEASY, X-PLOR_NIH         
REMARK 210   METHOD USED                   : SIMULATED ANNEALING, MOLECULAR     
REMARK 210                                   DYNAMICS                           
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 75                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 15                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST ENERGY  
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (RES=RESIDUE NAME;         
REMARK 470 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):         
REMARK 470   MODELS 1-15                                                        
REMARK 470     RES CSSEQI  ATOMS                                                
REMARK 470     LEU A  33    O                                                   
REMARK 470     LEU B  33    O                                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 VAL A   4      -76.01     56.64                                   
REMARK 500  1 SER A   5       79.95     69.26                                   
REMARK 500  1 LEU A  30       78.06   -174.11                                   
REMARK 500  1 ARG A  32       93.42    -49.53                                   
REMARK 500  1 SER B   2       -2.89     64.51                                   
REMARK 500  1 THR B   3       -7.18     66.89                                   
REMARK 500  1 VAL B   4       49.05     31.24                                   
REMARK 500  1 SER B   5       55.95   -156.65                                   
REMARK 500  1 ARG B  32       80.29     49.20                                   
REMARK 500  2 THR A   3      -98.98    -62.76                                   
REMARK 500  2 LEU A  30       78.10   -174.38                                   
REMARK 500  2 ARG A  32      101.35    -54.82                                   
REMARK 500  2 SER B   2       15.40     46.18                                   
REMARK 500  2 THR B   3      160.71     56.06                                   
REMARK 500  2 SER B   5       81.10   -156.54                                   
REMARK 500  2 ARG B  32       86.33     51.00                                   
REMARK 500  3 VAL A   4       -7.23    -58.81                                   
REMARK 500  3 SER A   5       60.35     63.94                                   
REMARK 500  3 LEU A  30       78.71   -175.15                                   
REMARK 500  3 SER B   2      134.39     63.81                                   
REMARK 500  3 THR B   3      113.80   -160.09                                   
REMARK 500  3 SER B   5       48.52   -173.31                                   
REMARK 500  3 PRO B   6       88.11    -66.95                                   
REMARK 500  3 ARG B  32       87.14    -60.16                                   
REMARK 500  4 THR A   3      -82.07     52.56                                   
REMARK 500  4 SER A   5       69.51     39.62                                   
REMARK 500  4 LEU A  30       78.02   -174.48                                   
REMARK 500  4 SER B   2       26.48     47.40                                   
REMARK 500  4 THR B   3      120.78     59.34                                   
REMARK 500  4 SER B   5       71.99   -172.56                                   
REMARK 500  4 PRO B   6       86.45    -46.31                                   
REMARK 500  5 SER A   2      -74.21    -76.59                                   
REMARK 500  5 THR A   3      -87.63   -167.27                                   
REMARK 500  5 VAL A   4       96.80     41.23                                   
REMARK 500  5 PRO A   6       69.59    -64.80                                   
REMARK 500  5 LEU A  30       78.73   -175.39                                   
REMARK 500  5 SER B   2      -17.34     71.77                                   
REMARK 500  5 VAL B   4       29.53     34.20                                   
REMARK 500  5 SER B   5       72.46   -166.59                                   
REMARK 500  6 LEU A  30       77.57   -173.71                                   
REMARK 500  6 SER B   2      -30.66     73.65                                   
REMARK 500  6 THR B   3       99.61     54.71                                   
REMARK 500  6 ARG B  32       80.13     50.98                                   
REMARK 500  7 SER A   2      -88.18   -151.14                                   
REMARK 500  7 THR A   3     -144.97    -62.84                                   
REMARK 500  7 LEU A  30       76.47   -172.40                                   
REMARK 500  7 SER B   2       24.90     44.51                                   
REMARK 500  7 VAL B   4       72.34     39.06                                   
REMARK 500  7 PRO B   6      101.29    -46.13                                   
REMARK 500  7 ARG B  32       87.11    -64.36                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      99 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 2HAC   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF ZETA-ZETA TRANSMEMBRANE DIMER                           
REMARK 900 RELATED ID: 2K4F   RELATED DB: PDB                                   
REMARK 900 MOUSE CD3EPSILON CYTOPLASMIC TAIL                                    
REMARK 900 RELATED ID: 2L35   RELATED DB: PDB                                   
DBREF  2L34 A    1    33  UNP    O43914   TYOBP_HUMAN     35     67             
DBREF  2L34 B    1    33  UNP    O43914   TYOBP_HUMAN     35     67             
SEQADV 2L34 VAL A   14  UNP  O43914    MET    48 CONFLICT                       
SEQADV 2L34 VAL B   14  UNP  O43914    MET    48 CONFLICT                       
SEQRES   1 A   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 A   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 A   33  VAL TYR PHE LEU GLY ARG LEU                                  
SEQRES   1 B   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 B   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 B   33  VAL TYR PHE LEU GLY ARG LEU                                  
HELIX    1   1 PRO A    6  GLY A   31  1                                  26    
HELIX    2   2 CYS B    1  SER B    5  5                                   5    
HELIX    3   3 PRO B    6  ARG B   32  1                                  27    
SSBOND   1 CYS A    1    CYS B    1                          1555   1555  2.02  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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