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Database: PDB
Entry: 2LBF
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HEADER    RIBOSOMAL PROTEIN                       30-MAR-11   2LBF              
TITLE     SOLUTION STRUCTURE OF THE DIMERIZATION DOMAIN OF HUMAN RIBOSOMAL      
TITLE    2 PROTEIN P1/P2 HETERODIMER                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: 60S ACIDIC RIBOSOMAL PROTEIN P1;                           
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 1-69;                                         
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: 60S ACIDIC RIBOSOMAL PROTEIN P2;                           
COMPND   8 CHAIN: B;                                                            
COMPND   9 FRAGMENT: UNP RESIDUES 1-69;                                         
COMPND  10 SYNONYM: RENAL CARCINOMA ANTIGEN NY-REN-44;                          
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: RPLP1, RRP1;                                                   
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: VECTOR;                               
SOURCE   9 EXPRESSION_SYSTEM_VECTOR: PET3D;                                     
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_COMMON: HUMAN;                                              
SOURCE  13 ORGANISM_TAXID: 9606;                                                
SOURCE  14 GENE: RPLP2, D11S2243E, RPP2;                                        
SOURCE  15 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  16 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  17 EXPRESSION_SYSTEM_VECTOR_TYPE: VECTOR;                               
SOURCE  18 EXPRESSION_SYSTEM_VECTOR: PRSETA                                     
KEYWDS    RIBOSOME, STALK, P1/P2, RIBOSOMAL PROTEIN                             
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    K.-M.LEE,C.W.-H.YU,T.Y.-H.CHIU,K.-H.SZE,P.-C.SHAW,K.-B.WONG           
REVDAT   1   14-DEC-11 2LBF    0                                                
JRNL        AUTH   K.-M.LEE,C.W.-H.YU,T.Y.-H.CHIU,K.-H.SZE,P.-C.SHAW,K.-B.WONG  
JRNL        TITL   SOLUTION STRUCTURE OF THE DIMERIZATION DOMAIN OF THE         
JRNL        TITL 2 EUKARYOTIC STALK P1/P2 COMPLEX REVEALS THE STRUCTURAL        
JRNL        TITL 3 ORGANIZATION OF EUKARYOTIC STALK COMPLEX                     
JRNL        REF    NUCLEIC ACIDS RES.                         2011              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   22135285                                                     
JRNL        DOI    10.1093/NAR/GKR1143                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS                                                  
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-KUNSTLEVE,    
REMARK   3  JIANG,KUSZEWSKI,NILGES,PANNU,READ,RICE,SIMONSON,WARREN              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2LBF COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 12-APR-11.                  
REMARK 100 THE RCSB ID CODE IS RCSB102190.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 6.5                                
REMARK 210  IONIC STRENGTH                 : 0.15                               
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1 MM [U-100% 13C; U-100% 15N]      
REMARK 210                                   PROTEIN_1-1, 1 MM [U-100% 13C; U-  
REMARK 210                                   100% 15N] PROTEIN_2-2, 90% H2O/    
REMARK 210                                   10% D2O                            
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC                     
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : ARIA                               
REMARK 210   METHOD USED                   : DGSA-DISTANCE GEOMETRY SIMULATED   
REMARK 210                                   ANNEALING                          
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   HB   VAL A    37     HA3  GLY B   113              1.25            
REMARK 500   HB2  TYR B   103    HG12  VAL B   142              1.27            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ALA A   2     -167.59   -115.83                                   
REMARK 500  1 ASN A  53      -41.71   -143.01                                   
REMARK 500  1 ASN A  62       59.81     35.25                                   
REMARK 500  1 VAL A  63       43.70     34.19                                   
REMARK 500  1 ARG B 102     -162.84     79.36                                   
REMARK 500  1 SER B 117       68.88   -168.01                                   
REMARK 500  1 LEU B 162      116.82    -38.08                                   
REMARK 500  1 ALA B 163      -78.29     46.39                                   
REMARK 500  1 PRO B 166      115.74    -16.99                                   
REMARK 500  2 LEU A  51       45.47    -74.21                                   
REMARK 500  2 VAL A  63       32.67    -80.23                                   
REMARK 500  2 ARG B 102      -92.15   -152.99                                   
REMARK 500  2 SER B 116       26.80    -77.04                                   
REMARK 500  2 SER B 117       70.39   -168.80                                   
REMARK 500  2 LEU B 162      123.39    -29.12                                   
REMARK 500  2 ALA B 163      -68.58     61.05                                   
REMARK 500  2 PRO B 166      116.44    -12.51                                   
REMARK 500  3 ALA A   2      149.38    176.35                                   
REMARK 500  3 ASN A  53      -48.20   -143.62                                   
REMARK 500  3 ASN A  62      -13.83     54.53                                   
REMARK 500  3 VAL A  63       34.62    -82.60                                   
REMARK 500  3 ARG B 102      125.90   -170.02                                   
REMARK 500  3 TYR B 103      -25.66     71.66                                   
REMARK 500  3 SER B 116       26.01    -79.97                                   
REMARK 500  3 SER B 117       69.82   -172.31                                   
REMARK 500  3 ILE B 155       44.10   -109.15                                   
REMARK 500  3 LEU B 162     -162.70     45.03                                   
REMARK 500  3 ALA B 163      -90.85     50.56                                   
REMARK 500  3 PRO B 166      110.65    -17.10                                   
REMARK 500  4 ASN A  53      -52.80   -139.52                                   
REMARK 500  4 ARG B 102      -91.12   -172.52                                   
REMARK 500  4 SER B 116       31.09    -80.23                                   
REMARK 500  4 SER B 117       67.64   -171.00                                   
REMARK 500  4 GLN B 157      -34.83   -137.36                                   
REMARK 500  4 SER B 164      159.13     62.43                                   
REMARK 500  4 PRO B 166      121.13    -13.70                                   
REMARK 500  5 THR A  22      127.97    -34.96                                   
REMARK 500  5 LEU A  51        0.77    -66.90                                   
REMARK 500  5 ASN A  53      -38.26   -130.69                                   
REMARK 500  5 PRO A  68       38.63    -86.55                                   
REMARK 500  5 ARG B 102     -147.26   -170.24                                   
REMARK 500  5 SER B 116       25.61    -76.96                                   
REMARK 500  5 SER B 117       68.12   -169.83                                   
REMARK 500  5 GLN B 157       16.08   -142.05                                   
REMARK 500  5 LEU B 162      175.88     48.27                                   
REMARK 500  5 ALA B 163      -88.57     55.13                                   
REMARK 500  5 PRO B 166      114.85    -19.63                                   
REMARK 500  6 ARG B 102     -122.74   -162.74                                   
REMARK 500  6 SER B 117       67.96   -175.78                                   
REMARK 500  6 SER B 164      169.49     56.90                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      85 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 17557   RELATED DB: BMRB                                 
DBREF  2LBF A    1    69  UNP    P05386   RLA1_HUMAN       1     69             
DBREF  2LBF B  101   169  UNP    P05387   RLA2_HUMAN       1     69             
SEQADV 2LBF ALA B  100  UNP  P05387              EXPRESSION TAG                 
SEQRES   1 A   69  MET ALA SER VAL SER GLU LEU ALA CYS ILE TYR SER ALA          
SEQRES   2 A   69  LEU ILE LEU HIS ASP ASP GLU VAL THR VAL THR GLU ASP          
SEQRES   3 A   69  LYS ILE ASN ALA LEU ILE LYS ALA ALA GLY VAL ASN VAL          
SEQRES   4 A   69  GLU PRO PHE TRP PRO GLY LEU PHE ALA LYS ALA LEU ALA          
SEQRES   5 A   69  ASN VAL ASN ILE GLY SER LEU ILE CYS ASN VAL GLY ALA          
SEQRES   6 A   69  GLY GLY PRO ALA                                              
SEQRES   1 B   70  ALA MET ARG TYR VAL ALA SER TYR LEU LEU ALA ALA LEU          
SEQRES   2 B   70  GLY GLY ASN SER SER PRO SER ALA LYS ASP ILE LYS LYS          
SEQRES   3 B   70  ILE LEU ASP SER VAL GLY ILE GLU ALA ASP ASP ASP ARG          
SEQRES   4 B   70  LEU ASN LYS VAL ILE SER GLU LEU ASN GLY LYS ASN ILE          
SEQRES   5 B   70  GLU ASP VAL ILE ALA GLN GLY ILE GLY LYS LEU ALA SER          
SEQRES   6 B   70  VAL PRO ALA GLY GLY                                          
HELIX    1   1 SER A    3  GLU A   20  1                                  18    
HELIX    2   2 THR A   24  GLY A   36  1                                  13    
HELIX    3   3 PRO A   41  LEU A   51  1                                  11    
HELIX    4   4 ASN A   55  CYS A   61  5                                   7    
HELIX    5   5 TYR B  103  GLY B  114  1                                  12    
HELIX    6   6 SER B  119  SER B  129  1                                  11    
HELIX    7   7 ASP B  137  ASN B  147  1                                  11    
HELIX    8   8 ASN B  150  ALA B  156  1                                   7    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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