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Database: PDB
Entry: 2MFN
LinkDB: 2MFN
Original site: 2MFN 
HEADER    CELL ADHESION PROTEIN                   11-FEB-98   2MFN              
TITLE     SOLUTION NMR STRUCTURE OF LINKED CELL ATTACHMENT MODULES OF           
TITLE    2 MOUSE FIBRONECTIN CONTAINING THE RGD AND SYNERGY REGIONS,            
TITLE    3 10 STRUCTURES                                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: FIBRONECTIN;                                               
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: 184 AMINO ACID FRAGMENT, 9TH AND 10TH TYPE-III             
COMPND   5 REPEATS;                                                             
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;                                        
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 CELL_LINE: BL21;                                                     
SOURCE   6 GENE: POTENTIAL;                                                     
SOURCE   7 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   8 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   9 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3) PLYSS;                          
SOURCE  10 EXPRESSION_SYSTEM_VECTOR: PRSET;                                     
SOURCE  11 EXPRESSION_SYSTEM_PLASMID: BL21                                      
KEYWDS    CELL ADHESION PROTEIN, RGD, EXTRACELLULAR MATRIX                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    V.COPIE,Y.TOMITA,S.K.AKIYAMA,S.AOTA,K.M.YAMADA,R.M.VENABLE,           
AUTHOR   2 R.W.PASTOR,S.KRUEGER,D.A.TORCHIA                                     
REVDAT   2   24-FEB-09 2MFN    1       VERSN                                    
REVDAT   1   29-APR-98 2MFN    0                                                
JRNL        AUTH   V.COPIE,Y.TOMITA,S.K.AKIYAMA,S.AOTA,K.M.YAMADA,              
JRNL        AUTH 2 R.M.VENABLE,R.W.PASTOR,S.KRUEGER,D.A.TORCHIA                 
JRNL        TITL   SOLUTION STRUCTURE AND DYNAMICS OF LINKED CELL               
JRNL        TITL 2 ATTACHMENT MODULES OF MOUSE FIBRONECTIN CONTAINING           
JRNL        TITL 3 THE RGD AND SYNERGY REGIONS: COMPARISON WITH THE             
JRNL        TITL 4 HUMAN FIBRONECTIN CRYSTAL STRUCTURE.                         
JRNL        REF    J.MOL.BIOL.                   V. 277   663 1998              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   9533887                                                      
JRNL        DOI    10.1006/JMBI.1998.1616                                       
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.8                                           
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: REFINEMENT DETAILS CAN BE FOUND IN        
REMARK   3  THE JRNL CITATION ABOVE.                                            
REMARK   4                                                                      
REMARK   4 2MFN COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 308                                
REMARK 210  PH                             : 4.2                                
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : TRIPLE RESONANCE FOR               
REMARK 210                                   ASSIGNMENT OF PROTEIN:             
REMARK 210                                   CBCA(CO)NH, CBCANH, HBHA(CO)       
REMARK 210                                   NH, C (CO)NH, H(CCO)NH, HCCH-      
REMARK 210                                   TOCSY, HOHAHA, 15N, 13C-HSQC,      
REMARK 210                                   2D, 3D, 4D-NOESY. QUANTITATIVE     
REMARK 210                                   J CORRELATION FOR COUPLING         
REMARK 210                                   CONSTANTS; HNHA, HNHB, HAHB,       
REMARK 210                                   CCO-SED, CN-SED, LRCC, LRCH.       
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : DMX500, AMX500, AMX600             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : X-PLOR                             
REMARK 210   METHOD USED                   : TORSION-ANGLE MOLECULAR            
REMARK 210                                   DYNAMICS                           
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 40                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NO NOE VIOLATIONS GREATER          
REMARK 210                                   THAN 0.5 A,NO DIHEDRAL ANGLE       
REMARK 210                                   RESTRAINT VIOLATIONS > 5, RMSD     
REMARK 210                                   FOR BOND DEVIATIONS FROM           
REMARK 210                                   IDEALITY < 0.05 A, RMSD FOR        
REMARK 210                                   ANGLE DEVIATIONS FROM IDEALITY     
REMARK 210                                   < 5 AND RMSD FOR IMPROPER          
REMARK 210                                   ANGLES DEVIATIONS FROM             
REMARK 210                                   IDEALITY < 5                       
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OG1  THR A   104     O    SER A   107              2.16            
REMARK 500   OG   SER A    10     OE1  GLN A    88              2.18            
REMARK 500   OG1  THR A    14     O    SER A    17              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 THR A   6     -153.29   -150.57                                   
REMARK 500  1 PRO A  25      176.15    -59.17                                   
REMARK 500  1 VAL A  91      171.68    -43.47                                   
REMARK 500  1 PRO A  95     -165.97    -58.45                                   
REMARK 500  1 PRO A 115     -162.18    -60.87                                   
REMARK 500  1 THR A 129      -89.10    -21.37                                   
REMARK 500  1 PRO A 134       44.33    -65.91                                   
REMARK 500  1 PRO A 141       44.23    -59.80                                   
REMARK 500  1 ASN A 151       44.98     77.15                                   
REMARK 500  1 THR A 166      -65.30   -108.52                                   
REMARK 500  1 PRO A 172       99.03    -66.34                                   
REMARK 500  2 PRO A   5       72.65    -58.81                                   
REMARK 500  2 THR A   6     -153.73    -79.55                                   
REMARK 500  2 ASN A  76       38.45   -140.49                                   
REMARK 500  2 PRO A  95     -170.37    -58.78                                   
REMARK 500  2 ASP A  97       36.22     72.60                                   
REMARK 500  2 PRO A 115     -160.65    -60.63                                   
REMARK 500  2 THR A 129      -35.39    -28.91                                   
REMARK 500  2 THR A 166      -65.38   -123.13                                   
REMARK 500  3 PRO A   5       79.91    -59.04                                   
REMARK 500  3 THR A   6     -159.56    -85.90                                   
REMARK 500  3 VAL A  91      171.26    -37.21                                   
REMARK 500  3 PRO A  95     -158.61    -61.70                                   
REMARK 500  3 ASP A  97       35.08     74.96                                   
REMARK 500  3 PRO A 115     -163.42    -63.31                                   
REMARK 500  3 PRO A 141       50.56    -58.87                                   
REMARK 500  3 ARG A 168     -109.98   -114.87                                   
REMARK 500  3 PRO A 172       82.61    -65.74                                   
REMARK 500  3 LYS A 176      100.10    -39.07                                   
REMARK 500  4 THR A   6     -144.36   -156.20                                   
REMARK 500  4 PRO A  25     -179.50    -59.79                                   
REMARK 500  4 GLU A  39      -97.27    -72.66                                   
REMARK 500  4 PRO A  45       95.47    -61.75                                   
REMARK 500  4 VAL A  91      166.62    -45.72                                   
REMARK 500  4 PRO A 115     -178.22    -60.22                                   
REMARK 500  4 PRO A 134       87.15    -57.21                                   
REMARK 500  4 PRO A 172       98.57    -62.06                                   
REMARK 500  5 THR A   6     -150.65   -143.54                                   
REMARK 500  5 PRO A  25     -164.57    -61.58                                   
REMARK 500  5 VAL A  91      155.97    -41.71                                   
REMARK 500  5 ASP A  93       14.61     58.62                                   
REMARK 500  5 PRO A 115     -166.33    -60.03                                   
REMARK 500  5 THR A 129      157.95    -33.08                                   
REMARK 500  5 ASN A 151       49.74     71.69                                   
REMARK 500  5 PRO A 172       87.66    -64.02                                   
REMARK 500  6 THR A   6     -155.75   -120.00                                   
REMARK 500  6 SER A  11     -162.32   -172.75                                   
REMARK 500  6 PRO A  25      179.28    -58.69                                   
REMARK 500  6 VAL A  91      170.21    -55.33                                   
REMARK 500  6 PRO A  95       81.31    -63.68                                   
REMARK 500  6 THR A 129      -64.68    -17.26                                   
REMARK 500  6 PRO A 134       86.47    -59.25                                   
REMARK 500  6 PRO A 172     -174.94    -62.24                                   
REMARK 500  7 THR A   6     -158.16   -152.68                                   
REMARK 500  7 GLU A  39      -87.13    -50.98                                   
REMARK 500  7 HIS A  40       27.60   -141.17                                   
REMARK 500  7 VAL A  91      171.43    -43.80                                   
REMARK 500  7 PRO A  95     -166.34    -66.17                                   
REMARK 500  7 PRO A 115     -171.93    -59.64                                   
REMARK 500  7 THR A 129      -86.43    -25.31                                   
REMARK 500  7 ASN A 132       19.79     39.95                                   
REMARK 500  7 PRO A 172     -160.15    -63.93                                   
REMARK 500  8 PRO A   5       90.34    -57.22                                   
REMARK 500  8 THR A   6     -158.54    -92.26                                   
REMARK 500  8 PRO A  95     -159.03    -64.42                                   
REMARK 500  8 PRO A 115     -159.29    -59.94                                   
REMARK 500  8 THR A 129      -20.01    -39.56                                   
REMARK 500  8 PRO A 141       35.65    -59.86                                   
REMARK 500  8 THR A 166      -69.30   -121.29                                   
REMARK 500  9 THR A   6     -155.29   -115.10                                   
REMARK 500  9 SER A  11     -166.03   -167.20                                   
REMARK 500  9 PRO A  25      176.01    -57.95                                   
REMARK 500  9 VAL A  91      173.07    -51.13                                   
REMARK 500  9 PRO A  95     -171.39    -57.42                                   
REMARK 500  9 ASP A  97       34.88     72.55                                   
REMARK 500  9 PRO A 115     -163.00    -60.38                                   
REMARK 500  9 GLU A 128       64.86   -109.21                                   
REMARK 500  9 PRO A 141       35.91    -62.35                                   
REMARK 500  9 THR A 166      -61.73   -129.82                                   
REMARK 500 10 PRO A   5       78.86    -57.76                                   
REMARK 500 10 THR A   6     -152.02    -81.55                                   
REMARK 500 10 PRO A  25     -178.46    -58.55                                   
REMARK 500 10 PRO A  95       62.13    -65.89                                   
REMARK 500 10 ARG A  96      163.93    -40.58                                   
REMARK 500 10 THR A 129      -62.27    -22.80                                   
REMARK 500 10 SER A 133       35.04     75.51                                   
REMARK 500 10 PRO A 134       50.94    -60.17                                   
REMARK 500 10 PRO A 141       47.53    -60.05                                   
REMARK 500 10 THR A 166      -70.75   -116.73                                   
REMARK 500 10 PRO A 172     -177.68    -64.40                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A  26         0.10    SIDE_CHAIN                              
REMARK 500  1 ARG A  35         0.32    SIDE_CHAIN                              
REMARK 500  1 ARG A  44         0.20    SIDE_CHAIN                              
REMARK 500  1 ARG A  46         0.31    SIDE_CHAIN                              
REMARK 500  1 ARG A  54         0.18    SIDE_CHAIN                              
REMARK 500  1 ARG A  78         0.17    SIDE_CHAIN                              
REMARK 500  1 ARG A  96         0.31    SIDE_CHAIN                              
REMARK 500  1 ARG A 123         0.24    SIDE_CHAIN                              
REMARK 500  1 ARG A 168         0.30    SIDE_CHAIN                              
REMARK 500  2 ARG A  26         0.18    SIDE_CHAIN                              
REMARK 500  2 ARG A  35         0.14    SIDE_CHAIN                              
REMARK 500  2 ARG A  44         0.24    SIDE_CHAIN                              
REMARK 500  2 ARG A  46         0.09    SIDE_CHAIN                              
REMARK 500  2 ARG A  49         0.20    SIDE_CHAIN                              
REMARK 500  2 ARG A  54         0.16    SIDE_CHAIN                              
REMARK 500  2 ARG A  78         0.13    SIDE_CHAIN                              
REMARK 500  2 ARG A 120         0.26    SIDE_CHAIN                              
REMARK 500  2 ARG A 123         0.21    SIDE_CHAIN                              
REMARK 500  2 ARG A 168         0.18    SIDE_CHAIN                              
REMARK 500  3 ARG A  26         0.18    SIDE_CHAIN                              
REMARK 500  3 ARG A  35         0.22    SIDE_CHAIN                              
REMARK 500  3 ARG A  44         0.18    SIDE_CHAIN                              
REMARK 500  3 ARG A  46         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  54         0.24    SIDE_CHAIN                              
REMARK 500  3 ARG A  78         0.26    SIDE_CHAIN                              
REMARK 500  3 ARG A  96         0.18    SIDE_CHAIN                              
REMARK 500  3 ARG A 120         0.30    SIDE_CHAIN                              
REMARK 500  3 ARG A 123         0.27    SIDE_CHAIN                              
REMARK 500  4 ARG A  46         0.18    SIDE_CHAIN                              
REMARK 500  4 ARG A  49         0.20    SIDE_CHAIN                              
REMARK 500  4 ARG A  54         0.12    SIDE_CHAIN                              
REMARK 500  4 ARG A  78         0.31    SIDE_CHAIN                              
REMARK 500  4 ARG A  96         0.32    SIDE_CHAIN                              
REMARK 500  4 ARG A 120         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG A 123         0.15    SIDE_CHAIN                              
REMARK 500  4 ARG A 168         0.14    SIDE_CHAIN                              
REMARK 500  5 ARG A  26         0.24    SIDE_CHAIN                              
REMARK 500  5 ARG A  35         0.30    SIDE_CHAIN                              
REMARK 500  5 ARG A  44         0.17    SIDE_CHAIN                              
REMARK 500  5 ARG A  46         0.12    SIDE_CHAIN                              
REMARK 500  5 ARG A  49         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG A  54         0.27    SIDE_CHAIN                              
REMARK 500  5 ARG A  78         0.26    SIDE_CHAIN                              
REMARK 500  5 ARG A  96         0.09    SIDE_CHAIN                              
REMARK 500  5 ARG A 120         0.26    SIDE_CHAIN                              
REMARK 500  5 ARG A 123         0.20    SIDE_CHAIN                              
REMARK 500  5 ARG A 168         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG A  26         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG A  35         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG A  44         0.26    SIDE_CHAIN                              
REMARK 500  6 ARG A  46         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG A  49         0.19    SIDE_CHAIN                              
REMARK 500  6 ARG A  54         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG A  78         0.24    SIDE_CHAIN                              
REMARK 500  6 ARG A  96         0.17    SIDE_CHAIN                              
REMARK 500  6 ARG A 120         0.25    SIDE_CHAIN                              
REMARK 500  6 ARG A 123         0.25    SIDE_CHAIN                              
REMARK 500  6 ARG A 168         0.12    SIDE_CHAIN                              
REMARK 500  7 ARG A  26         0.15    SIDE_CHAIN                              
REMARK 500  7 ARG A  35         0.11    SIDE_CHAIN                              
REMARK 500  7 ARG A  46         0.15    SIDE_CHAIN                              
REMARK 500  7 ARG A  49         0.26    SIDE_CHAIN                              
REMARK 500  7 ARG A  54         0.09    SIDE_CHAIN                              
REMARK 500  7 ARG A  78         0.32    SIDE_CHAIN                              
REMARK 500  7 ARG A  96         0.24    SIDE_CHAIN                              
REMARK 500  7 ARG A 168         0.30    SIDE_CHAIN                              
REMARK 500  8 ARG A  26         0.32    SIDE_CHAIN                              
REMARK 500  8 ARG A  35         0.31    SIDE_CHAIN                              
REMARK 500  8 ARG A  44         0.12    SIDE_CHAIN                              
REMARK 500  8 ARG A  46         0.31    SIDE_CHAIN                              
REMARK 500  8 ARG A  49         0.24    SIDE_CHAIN                              
REMARK 500  8 ARG A  54         0.31    SIDE_CHAIN                              
REMARK 500  8 ARG A  78         0.12    SIDE_CHAIN                              
REMARK 500  8 ARG A 120         0.12    SIDE_CHAIN                              
REMARK 500  8 ARG A 123         0.31    SIDE_CHAIN                              
REMARK 500  8 ARG A 168         0.22    SIDE_CHAIN                              
REMARK 500  9 ARG A  26         0.29    SIDE_CHAIN                              
REMARK 500  9 ARG A  35         0.15    SIDE_CHAIN                              
REMARK 500  9 ARG A  44         0.24    SIDE_CHAIN                              
REMARK 500  9 ARG A  46         0.10    SIDE_CHAIN                              
REMARK 500  9 ARG A  49         0.32    SIDE_CHAIN                              
REMARK 500  9 ARG A  54         0.10    SIDE_CHAIN                              
REMARK 500  9 ARG A  96         0.32    SIDE_CHAIN                              
REMARK 500  9 ARG A 120         0.29    SIDE_CHAIN                              
REMARK 500  9 ARG A 123         0.27    SIDE_CHAIN                              
REMARK 500  9 ARG A 168         0.23    SIDE_CHAIN                              
REMARK 500 10 ARG A  26         0.24    SIDE_CHAIN                              
REMARK 500 10 ARG A  44         0.30    SIDE_CHAIN                              
REMARK 500 10 ARG A  46         0.30    SIDE_CHAIN                              
REMARK 500 10 ARG A  49         0.29    SIDE_CHAIN                              
REMARK 500 10 ARG A  54         0.31    SIDE_CHAIN                              
REMARK 500 10 ARG A  78         0.32    SIDE_CHAIN                              
REMARK 500 10 ARG A  96         0.20    SIDE_CHAIN                              
REMARK 500 10 ARG A 120         0.26    SIDE_CHAIN                              
REMARK 500 10 ARG A 168         0.08    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: RGD                                                 
REMARK 800 EVIDENCE_CODE: UNKNOWN                                               
REMARK 800 SITE_DESCRIPTION: CELL ADHESION SITE.                                
REMARK 800 SITE_IDENTIFIER: SGY                                                 
REMARK 800 EVIDENCE_CODE: UNKNOWN                                               
REMARK 800 SITE_DESCRIPTION: CELL ADHESION SYNERGETIC SITE.                     
DBREF  2MFN A    1   184  UNP    P11276   FINC_MOUSE    1447   1630             
SEQRES   1 A  184  GLY LEU ASP SER PRO THR GLY PHE ASP SER SER ASP ILE          
SEQRES   2 A  184  THR ALA ASN SER PHE THR VAL HIS TRP VAL ALA PRO ARG          
SEQRES   3 A  184  ALA PRO ILE THR GLY TYR ILE ILE ARG HIS HIS ALA GLU          
SEQRES   4 A  184  HIS SER VAL GLY ARG PRO ARG GLN ASP ARG VAL PRO PRO          
SEQRES   5 A  184  SER ARG ASN SER ILE THR LEU THR ASN LEU ASN PRO GLY          
SEQRES   6 A  184  THR GLU TYR VAL VAL SER ILE ILE ALA VAL ASN GLY ARG          
SEQRES   7 A  184  GLU GLU SER PRO PRO LEU ILE GLY GLN GLN ALA THR VAL          
SEQRES   8 A  184  SER ASP ILE PRO ARG ASP LEU GLU VAL ILE ALA SER THR          
SEQRES   9 A  184  PRO THR SER LEU LEU ILE SER TRP GLU PRO PRO ALA VAL          
SEQRES  10 A  184  SER VAL ARG TYR TYR ARG ILE THR TYR GLY GLU THR GLY          
SEQRES  11 A  184  GLY ASN SER PRO VAL GLN GLU PHE THR VAL PRO GLY SER          
SEQRES  12 A  184  LYS SER THR ALA THR ILE ASN ASN ILE LYS PRO GLY ALA          
SEQRES  13 A  184  ASP TYR THR ILE THR LEU TYR ALA VAL THR GLY ARG GLY          
SEQRES  14 A  184  ASP SER PRO ALA SER SER LYS PRO VAL SER ILE ASN TYR          
SEQRES  15 A  184  LYS THR                                                      
SHEET    1   A 3 SER A  56  LEU A  59  0                                        
SHEET    2   A 3 PHE A  18  HIS A  21 -1  N  VAL A  20   O  ILE A  57           
SHEET    3   A 3 ASP A   9  ILE A  13 -1  N  ASP A  12   O  THR A  19           
SHEET    1   B 4 ARG A  46  VAL A  50  0                                        
SHEET    2   B 4 GLY A  31  ALA A  38 -1  N  HIS A  36   O  ARG A  46           
SHEET    3   B 4 THR A  66  VAL A  75 -1  N  VAL A  75   O  GLY A  31           
SHEET    4   B 4 LEU A  84  THR A  90 -1  N  THR A  90   O  THR A  66           
SHEET    1   C 3 THR A 146  ILE A 149  0                                        
SHEET    2   C 3 SER A 107  SER A 111 -1  N  ILE A 110   O  ALA A 147           
SHEET    3   C 3 GLU A  99  THR A 104 -1  N  THR A 104   O  SER A 107           
SHEET    1   D 4 GLN A 136  VAL A 140  0                                        
SHEET    2   D 4 TYR A 121  TYR A 126 -1  N  TYR A 126   O  GLN A 136           
SHEET    3   D 4 ASP A 157  VAL A 165 -1  N  VAL A 165   O  TYR A 121           
SHEET    4   D 4 VAL A 178  LYS A 183 -1  N  TYR A 182   O  TYR A 158           
SITE     1 RGD  3 ARG A 168  GLY A 169  ASP A 170                               
SITE     1 SGY  5 PRO A  51  PRO A  52  SER A  53  ARG A  54                    
SITE     2 SGY  5 ASN A  55                                                     
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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