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Database: PDB
Entry: 2N9D
LinkDB: 2N9D
Original site: 2N9D 
HEADER    TRANSPORT PROTEIN                       15-NOV-15   2N9D              
TITLE     STRUCTURE ANALYSIS OF THE TOM1 GAT DOMAIN REVEALS DISTINCT LIGAND-    
TITLE    2 SPECIFIC CONFORMATIONAL STATES                                       
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TARGET OF MYB PROTEIN 1;                                   
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: GAT DOMAIN (UNP RESIDUES 215-309);                         
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TOM1;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PGEX-6P-1                                 
KEYWDS    TRANSPORT PROTEIN, TOM1, EARLY ENDOSOMES, ALTERNATIVE ESCRT-0 MEMBER, 
KEYWDS   2 CARGO                                                                
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    S.XIAO,E.F.JEFFREY,A.S.GEOFFREY,D.G.S.CAPELLUTO                       
REVDAT   1   21-SEP-16 2N9D    0                                                
JRNL        AUTH   S.XIAO,J.F.ELLENA,G.S.ARMSTRONG,D.G.CAPELLUTO                
JRNL        TITL   STRUCTURE OF THE GAT DOMAIN OF THE ENDOSOMAL ADAPTER PROTEIN 
JRNL        TITL 2 TOM1.                                                        
JRNL        REF    DATA BRIEF                    V.   7   344 2016              
JRNL        REFN                                                                
JRNL        PMID   26977434                                                     
JRNL        DOI    10.1016/J.DIB.2016.02.042                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CS-ROSETTA                                           
REMARK   3   AUTHORS     : YANG, S. ET AL.                                      
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2N9D COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 16-NOV-15.                  
REMARK 100 THE RCSB ID CODE IS RCSB104586.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 70                                 
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : 1.0 MM [U-99% 13C; U-99% 15N]      
REMARK 210                                   GAT, 50 MM POTASSIUM CHLORIDE, 20  
REMARK 210                                   MM [U-2H] TRIS, 1 MM SODIUM        
REMARK 210                                   AZIDE, 50 UM DSS, 1 MM [U-2H]      
REMARK 210                                   DTT, 90% H2O/10% D2O               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC; 3D HNCO; 3D        
REMARK 210                                   HNCACB; 3D CBCA(CO)NH; 3D HCACO    
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : SPARKY                             
REMARK 210   METHOD USED                   : NULL                               
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 3000                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST ENERGY  
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;                   
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                            
REMARK 465   MODELS 1-10                                                        
REMARK 465     RES C SSSEQI                                                     
REMARK 465     GLY A   210                                                      
REMARK 465     PRO A   211                                                      
REMARK 465     LEU A   212                                                      
REMARK 465     GLY A   213                                                      
REMARK 465     SER A   214                                                      
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 26574   RELATED DB: BMRB                                 
DBREF  2N9D A  215   309  UNP    O60784   TOM1_HUMAN     215    309             
SEQADV 2N9D GLY A  210  UNP  O60784              EXPRESSION TAG                 
SEQADV 2N9D PRO A  211  UNP  O60784              EXPRESSION TAG                 
SEQADV 2N9D LEU A  212  UNP  O60784              EXPRESSION TAG                 
SEQADV 2N9D GLY A  213  UNP  O60784              EXPRESSION TAG                 
SEQADV 2N9D SER A  214  UNP  O60784              EXPRESSION TAG                 
SEQRES   1 A  100  GLY PRO LEU GLY SER GLU GLN ILE GLY LYS LEU ARG SER          
SEQRES   2 A  100  GLU LEU GLU MET VAL SER GLY ASN VAL ARG VAL MET SER          
SEQRES   3 A  100  GLU MET LEU THR GLU LEU VAL PRO THR GLN ALA GLU PRO          
SEQRES   4 A  100  ALA ASP LEU GLU LEU LEU GLN GLU LEU ASN ARG THR CYS          
SEQRES   5 A  100  ARG ALA MET GLN GLN ARG VAL LEU GLU LEU ILE PRO GLN          
SEQRES   6 A  100  ILE ALA ASN GLU GLN LEU THR GLU GLU LEU LEU ILE VAL          
SEQRES   7 A  100  ASN ASP ASN LEU ASN ASN VAL PHE LEU ARG HIS GLU ARG          
SEQRES   8 A  100  PHE GLU ARG PHE ARG THR GLY GLN THR                          
HELIX    1   1 GLU A  215  LEU A  241  1                                  27    
HELIX    2   2 GLU A  247  ILE A  272  1                                  26    
HELIX    3   3 ASN A  277  GLY A  307  1                                  31    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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