HEADER TRANSPORT PROTEIN 15-NOV-15 2N9D
TITLE STRUCTURE ANALYSIS OF THE TOM1 GAT DOMAIN REVEALS DISTINCT LIGAND-
TITLE 2 SPECIFIC CONFORMATIONAL STATES
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: TARGET OF MYB PROTEIN 1;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: GAT DOMAIN (UNP RESIDUES 215-309);
COMPND 5 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: TOM1;
SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PGEX-6P-1
KEYWDS TRANSPORT PROTEIN, TOM1, EARLY ENDOSOMES, ALTERNATIVE ESCRT-0 MEMBER,
KEYWDS 2 CARGO
EXPDTA SOLUTION NMR
NUMMDL 10
AUTHOR S.XIAO,E.F.JEFFREY,A.S.GEOFFREY,D.G.S.CAPELLUTO
REVDAT 1 21-SEP-16 2N9D 0
JRNL AUTH S.XIAO,J.F.ELLENA,G.S.ARMSTRONG,D.G.CAPELLUTO
JRNL TITL STRUCTURE OF THE GAT DOMAIN OF THE ENDOSOMAL ADAPTER PROTEIN
JRNL TITL 2 TOM1.
JRNL REF DATA BRIEF V. 7 344 2016
JRNL REFN
JRNL PMID 26977434
JRNL DOI 10.1016/J.DIB.2016.02.042
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : CS-ROSETTA
REMARK 3 AUTHORS : YANG, S. ET AL.
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2N9D COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 16-NOV-15.
REMARK 100 THE RCSB ID CODE IS RCSB104586.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 7.0
REMARK 210 IONIC STRENGTH : 70
REMARK 210 PRESSURE : 1 ATM
REMARK 210 SAMPLE CONTENTS : 1.0 MM [U-99% 13C; U-99% 15N]
REMARK 210 GAT, 50 MM POTASSIUM CHLORIDE, 20
REMARK 210 MM [U-2H] TRIS, 1 MM SODIUM
REMARK 210 AZIDE, 50 UM DSS, 1 MM [U-2H]
REMARK 210 DTT, 90% H2O/10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 2D 1H-15N HSQC; 3D HNCO; 3D
REMARK 210 HNCACB; 3D CBCA(CO)NH; 3D HCACO
REMARK 210 SPECTROMETER FIELD STRENGTH : 800 MHZ
REMARK 210 SPECTROMETER MODEL : AVANCE
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : SPARKY
REMARK 210 METHOD USED : NULL
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 3000
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 10
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LOWEST ENERGY
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465 MODELS 1-10
REMARK 465 RES C SSSEQI
REMARK 465 GLY A 210
REMARK 465 PRO A 211
REMARK 465 LEU A 212
REMARK 465 GLY A 213
REMARK 465 SER A 214
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 26574 RELATED DB: BMRB
DBREF 2N9D A 215 309 UNP O60784 TOM1_HUMAN 215 309
SEQADV 2N9D GLY A 210 UNP O60784 EXPRESSION TAG
SEQADV 2N9D PRO A 211 UNP O60784 EXPRESSION TAG
SEQADV 2N9D LEU A 212 UNP O60784 EXPRESSION TAG
SEQADV 2N9D GLY A 213 UNP O60784 EXPRESSION TAG
SEQADV 2N9D SER A 214 UNP O60784 EXPRESSION TAG
SEQRES 1 A 100 GLY PRO LEU GLY SER GLU GLN ILE GLY LYS LEU ARG SER
SEQRES 2 A 100 GLU LEU GLU MET VAL SER GLY ASN VAL ARG VAL MET SER
SEQRES 3 A 100 GLU MET LEU THR GLU LEU VAL PRO THR GLN ALA GLU PRO
SEQRES 4 A 100 ALA ASP LEU GLU LEU LEU GLN GLU LEU ASN ARG THR CYS
SEQRES 5 A 100 ARG ALA MET GLN GLN ARG VAL LEU GLU LEU ILE PRO GLN
SEQRES 6 A 100 ILE ALA ASN GLU GLN LEU THR GLU GLU LEU LEU ILE VAL
SEQRES 7 A 100 ASN ASP ASN LEU ASN ASN VAL PHE LEU ARG HIS GLU ARG
SEQRES 8 A 100 PHE GLU ARG PHE ARG THR GLY GLN THR
HELIX 1 1 GLU A 215 LEU A 241 1 27
HELIX 2 2 GLU A 247 ILE A 272 1 26
HELIX 3 3 ASN A 277 GLY A 307 1 31
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END