GenomeNet

Database: PDB
Entry: 2NTD
LinkDB: 2NTD
Original site: 2NTD 
HEADER    HORMONE/GROWTH FACTOR                   07-NOV-06   2NTD              
TITLE     HUMAN FIBROBLAST GROWTH FACTOR-1 (140 AMINO ACID FORM) WITH           
TITLE    2 CYS117VAL/PRO134CYS MUTATIONS                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ACIDIC FIBROBLAST GROWTH FACTOR 1;                         
COMPND   3 CHAIN: A, B, C, D;                                                   
COMPND   4 SYNONYM: HEPARIN-BINDING GROWTH FACTOR 1, HBGF-1, AFGF,              
COMPND   5 BETA-ENDOTHELIAL CELL GROWTH FACTOR, ECGF- BETA;                     
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: FGF1, FGFA;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    BETA-TREFOIL, HORMONE/GROWTH FACTOR COMPLEX                           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    V.K.DUBEY,M.BLABER                                                    
REVDAT   2   24-FEB-09 2NTD    1       VERSN                                    
REVDAT   1   24-JUL-07 2NTD    0                                                
JRNL        AUTH   V.K.DUBEY,J.LEE,T.SOMASUNDARAM,S.BLABER,M.BLABER             
JRNL        TITL   SPACKLING THE CRACK: STABILIZING HUMAN FIBROBLAST            
JRNL        TITL 2 GROWTH FACTOR-1 BY TARGETING THE N AND C TERMINUS            
JRNL        TITL 3 BETA-STRAND INTERACTIONS                                     
JRNL        REF    J.MOL.BIOL.                   V. 371   256 2007              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   17570396                                                     
JRNL        DOI    10.1016/J.JMB.2007.05.065                                    
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.52 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,             
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.52                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 20.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 3.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : 221276.810                     
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : 0.0000                         
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 79.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 20565                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.173                           
REMARK   3   FREE R VALUE                     : 0.229                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.800                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 991                             
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.007                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 6                            
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.52                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.66                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 46.20                        
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 1923                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2100                       
REMARK   3   BIN FREE R VALUE                    : 0.2890                       
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 5.10                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 103                          
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.028                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 4594                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 340                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 27.10                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 25.50                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 2.35000                                              
REMARK   3    B22 (A**2) : -4.23000                                             
REMARK   3    B33 (A**2) : 1.88000                                              
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.98000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.24                            
REMARK   3   ESD FROM SIGMAA              (A) : 0.23                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 5.00                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.34                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : 0.36                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.007                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.30                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 26.00                           
REMARK   3   IMPROPER ANGLES        (DEGREES) : 0.80                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED                                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 1.320 ; 1.500                
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 2.250 ; 2.000                
REMARK   3   SIDE-CHAIN BOND              (A**2) : 1.860 ; 2.000                
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 2.920 ; 2.500                
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : FLAT MODEL                                           
REMARK   3   KSOL        : 0.36                                                 
REMARK   3   BSOL        : 33.37                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : WATER.PARAM                                    
REMARK   3  PARAMETER FILE  3  : ION.PARAM                                      
REMARK   3  PARAMETER FILE  4  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP                                    
REMARK   3  TOPOLOGY FILE  2   : WATER.TOP                                      
REMARK   3  TOPOLOGY FILE  3   : ION.TOP                                        
REMARK   3  TOPOLOGY FILE  4   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2NTD COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-NOV-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB040277.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 22-JUL-05                          
REMARK 200  TEMPERATURE           (KELVIN) : 104                                
REMARK 200  PH                             : 7.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : ROTATING ANODE                     
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU RU200                       
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418                             
REMARK 200  MONOCHROMATOR                  : OSMIC MIRROR                       
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MAR CCD 165 MM                     
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-3000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-3000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25003                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.140                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 3.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.0                               
REMARK 200  DATA REDUNDANCY                : 5.100                              
REMARK 200  R MERGE                    (I) : 0.10100                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 13.7000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.56                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 95.3                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.35100                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.960                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: CNS                                                   
REMARK 200 STARTING MODEL: PDB ENTRY 1JY0                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 57.48                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.89                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 3.6M SODIUM FORMATE, PH 7.5, VAPOR       
REMARK 280  DIFFUSION, HANGING DROP, TEMPERATURE 298K                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y+1/2,-Z                                             
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       54.16500            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3, 4                                              
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 300 REMARK: THE BIOLOGICAL ASSEMBLY IS A MONOMER. THERE ARE FOUR         
REMARK 300 MONOMERS IN THE ASU (CHAIN IDENTIFIERS A, B, C, AND D).              
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 4                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     HIS A     1A                                                     
REMARK 465     SER A   138                                                      
REMARK 465     SER A   139                                                      
REMARK 465     ASP A   140                                                      
REMARK 465     SER B   138                                                      
REMARK 465     SER B   139                                                      
REMARK 465     ASP B   140                                                      
REMARK 465     HIS C     1A                                                     
REMARK 465     SER C   138                                                      
REMARK 465     SER C   139                                                      
REMARK 465     ASP C   140                                                      
REMARK 465     HIS D     1A                                                     
REMARK 465     SER D   138                                                      
REMARK 465     SER D   139                                                      
REMARK 465     ASP D   140                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A   7       32.70   -153.59                                   
REMARK 500    ASP A  32     -167.13   -164.18                                   
REMARK 500    GLU A  49      -62.78    -93.51                                   
REMARK 500    ASN A  92      -29.56     73.90                                   
REMARK 500    ASP B  32     -167.94   -161.27                                   
REMARK 500    GLU B  49      -87.75    -97.89                                   
REMARK 500    HIS B  93      -65.71   -155.91                                   
REMARK 500    HIS B 124      158.85    174.96                                   
REMARK 500    PRO B 136     -151.79    -61.43                                   
REMARK 500    ASN C   7       40.09   -155.97                                   
REMARK 500    ASN C  18      -71.72    -39.86                                   
REMARK 500    ASP C  32     -165.64   -163.71                                   
REMARK 500    ASN C  92      -21.92     72.86                                   
REMARK 500    HIS D   1F    -179.28    -62.80                                   
REMARK 500    ASP D  32     -165.46   -160.45                                   
REMARK 500    GLU D  49      -84.05    -94.10                                   
REMARK 500    HIS D  93      -54.99   -154.00                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1JY0   RELATED DB: PDB                                   
DBREF  2NTD A    1   140  UNP    P05230   FGF1_HUMAN      16    155             
DBREF  2NTD B    1   140  UNP    P05230   FGF1_HUMAN      16    155             
DBREF  2NTD C    1   140  UNP    P05230   FGF1_HUMAN      16    155             
DBREF  2NTD D    1   140  UNP    P05230   FGF1_HUMAN      16    155             
SEQADV 2NTD HIS A    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS A    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS A    1C UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS A    1D UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS A    1E UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS A    1F UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD VAL A  117  UNP  P05230    CYS   132 ENGINEERED                     
SEQADV 2NTD CYS A  134  UNP  P05230    PRO   149 ENGINEERED                     
SEQADV 2NTD HIS B    1A UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS B    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS B    1C UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS B    1D UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS B    1E UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS B    1F UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD VAL B  117  UNP  P05230    CYS   132 ENGINEERED                     
SEQADV 2NTD CYS B  134  UNP  P05230    PRO   149 ENGINEERED                     
SEQADV 2NTD HIS C    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS C    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS C    1C UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS C    1D UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS C    1E UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS C    1F UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD VAL C  117  UNP  P05230    CYS   132 ENGINEERED                     
SEQADV 2NTD CYS C  134  UNP  P05230    PRO   149 ENGINEERED                     
SEQADV 2NTD HIS D    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS D    1B UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS D    1C UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS D    1D UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS D    1E UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD HIS D    1F UNP  P05230              EXPRESSION TAG                 
SEQADV 2NTD VAL D  117  UNP  P05230    CYS   132 ENGINEERED                     
SEQADV 2NTD CYS D  134  UNP  P05230    PRO   149 ENGINEERED                     
SEQRES   1 A  146  HIS HIS HIS HIS HIS HIS PHE ASN LEU PRO PRO GLY ASN          
SEQRES   2 A  146  TYR LYS LYS PRO LYS LEU LEU TYR CYS SER ASN GLY GLY          
SEQRES   3 A  146  HIS PHE LEU ARG ILE LEU PRO ASP GLY THR VAL ASP GLY          
SEQRES   4 A  146  THR ARG ASP ARG SER ASP GLN HIS ILE GLN LEU GLN LEU          
SEQRES   5 A  146  SER ALA GLU SER VAL GLY GLU VAL TYR ILE LYS SER THR          
SEQRES   6 A  146  GLU THR GLY GLN TYR LEU ALA MET ASP THR ASP GLY LEU          
SEQRES   7 A  146  LEU TYR GLY SER GLN THR PRO ASN GLU GLU CYS LEU PHE          
SEQRES   8 A  146  LEU GLU ARG LEU GLU GLU ASN HIS TYR ASN THR TYR ILE          
SEQRES   9 A  146  SER LYS LYS HIS ALA GLU LYS ASN TRP PHE VAL GLY LEU          
SEQRES  10 A  146  LYS LYS ASN GLY SER VAL LYS ARG GLY PRO ARG THR HIS          
SEQRES  11 A  146  TYR GLY GLN LYS ALA ILE LEU PHE LEU CYS LEU PRO VAL          
SEQRES  12 A  146  SER SER ASP                                                  
SEQRES   1 B  146  HIS HIS HIS HIS HIS HIS PHE ASN LEU PRO PRO GLY ASN          
SEQRES   2 B  146  TYR LYS LYS PRO LYS LEU LEU TYR CYS SER ASN GLY GLY          
SEQRES   3 B  146  HIS PHE LEU ARG ILE LEU PRO ASP GLY THR VAL ASP GLY          
SEQRES   4 B  146  THR ARG ASP ARG SER ASP GLN HIS ILE GLN LEU GLN LEU          
SEQRES   5 B  146  SER ALA GLU SER VAL GLY GLU VAL TYR ILE LYS SER THR          
SEQRES   6 B  146  GLU THR GLY GLN TYR LEU ALA MET ASP THR ASP GLY LEU          
SEQRES   7 B  146  LEU TYR GLY SER GLN THR PRO ASN GLU GLU CYS LEU PHE          
SEQRES   8 B  146  LEU GLU ARG LEU GLU GLU ASN HIS TYR ASN THR TYR ILE          
SEQRES   9 B  146  SER LYS LYS HIS ALA GLU LYS ASN TRP PHE VAL GLY LEU          
SEQRES  10 B  146  LYS LYS ASN GLY SER VAL LYS ARG GLY PRO ARG THR HIS          
SEQRES  11 B  146  TYR GLY GLN LYS ALA ILE LEU PHE LEU CYS LEU PRO VAL          
SEQRES  12 B  146  SER SER ASP                                                  
SEQRES   1 C  146  HIS HIS HIS HIS HIS HIS PHE ASN LEU PRO PRO GLY ASN          
SEQRES   2 C  146  TYR LYS LYS PRO LYS LEU LEU TYR CYS SER ASN GLY GLY          
SEQRES   3 C  146  HIS PHE LEU ARG ILE LEU PRO ASP GLY THR VAL ASP GLY          
SEQRES   4 C  146  THR ARG ASP ARG SER ASP GLN HIS ILE GLN LEU GLN LEU          
SEQRES   5 C  146  SER ALA GLU SER VAL GLY GLU VAL TYR ILE LYS SER THR          
SEQRES   6 C  146  GLU THR GLY GLN TYR LEU ALA MET ASP THR ASP GLY LEU          
SEQRES   7 C  146  LEU TYR GLY SER GLN THR PRO ASN GLU GLU CYS LEU PHE          
SEQRES   8 C  146  LEU GLU ARG LEU GLU GLU ASN HIS TYR ASN THR TYR ILE          
SEQRES   9 C  146  SER LYS LYS HIS ALA GLU LYS ASN TRP PHE VAL GLY LEU          
SEQRES  10 C  146  LYS LYS ASN GLY SER VAL LYS ARG GLY PRO ARG THR HIS          
SEQRES  11 C  146  TYR GLY GLN LYS ALA ILE LEU PHE LEU CYS LEU PRO VAL          
SEQRES  12 C  146  SER SER ASP                                                  
SEQRES   1 D  146  HIS HIS HIS HIS HIS HIS PHE ASN LEU PRO PRO GLY ASN          
SEQRES   2 D  146  TYR LYS LYS PRO LYS LEU LEU TYR CYS SER ASN GLY GLY          
SEQRES   3 D  146  HIS PHE LEU ARG ILE LEU PRO ASP GLY THR VAL ASP GLY          
SEQRES   4 D  146  THR ARG ASP ARG SER ASP GLN HIS ILE GLN LEU GLN LEU          
SEQRES   5 D  146  SER ALA GLU SER VAL GLY GLU VAL TYR ILE LYS SER THR          
SEQRES   6 D  146  GLU THR GLY GLN TYR LEU ALA MET ASP THR ASP GLY LEU          
SEQRES   7 D  146  LEU TYR GLY SER GLN THR PRO ASN GLU GLU CYS LEU PHE          
SEQRES   8 D  146  LEU GLU ARG LEU GLU GLU ASN HIS TYR ASN THR TYR ILE          
SEQRES   9 D  146  SER LYS LYS HIS ALA GLU LYS ASN TRP PHE VAL GLY LEU          
SEQRES  10 D  146  LYS LYS ASN GLY SER VAL LYS ARG GLY PRO ARG THR HIS          
SEQRES  11 D  146  TYR GLY GLN LYS ALA ILE LEU PHE LEU CYS LEU PRO VAL          
SEQRES  12 D  146  SER SER ASP                                                  
HET    FMT  A 141       3                                                       
HET    FMT  A 142       3                                                       
HET    FMT  A 143       3                                                       
HET    FMT  B 141       3                                                       
HET    FMT  B 142       3                                                       
HET    FMT  B 143       3                                                       
HET    FMT  D 141       3                                                       
HET    FMT  C 141       3                                                       
HET    FMT  D 142       3                                                       
HET    FMT  C 142       3                                                       
HET    FMT  C 143       3                                                       
HETNAM     FMT FORMIC ACID                                                      
FORMUL   5  FMT    11(C H2 O2)                                                  
FORMUL  16  HOH   *307(H2 O)                                                    
HELIX    1   1 HIS A  102  ASN A  106  5                                   5    
HELIX    2   2 ARG A  119  THR A  123  5                                   5    
HELIX    3   3 HIS B  102  ASN B  106  5                                   5    
HELIX    4   4 ARG B  119  THR B  123  5                                   5    
HELIX    5   5 GLN B  127  ILE B  130  5                                   4    
HELIX    6   6 HIS C  102  ASN C  106  5                                   5    
HELIX    7   7 ARG C  119  THR C  123  5                                   5    
HELIX    8   8 HIS D  102  ASN D  106  5                                   5    
HELIX    9   9 ARG D  119  THR D  123  5                                   5    
SHEET    1   A 9 VAL A  31  THR A  34  0                                        
SHEET    2   A 9 HIS A  21  ILE A  25 -1  N  PHE A  22   O  THR A  34           
SHEET    3   A 9 LYS A  12  CYS A  16 -1  N  CYS A  16   O  HIS A  21           
SHEET    4   A 9 PHE A 132  PRO A 136 -1  O  LEU A 135   N  LEU A  13           
SHEET    5   A 9 TYR A  94  SER A  99 -1  N  ASN A  95   O  PHE A 132           
SHEET    6   A 9 LEU A  84  GLU A  90 -1  N  LEU A  86   O  ILE A  98           
SHEET    7   A 9 GLU A  53  SER A  58 -1  N  VAL A  54   O  PHE A  85           
SHEET    8   A 9 GLN A  43  ALA A  48 -1  N  GLN A  45   O  LYS A  57           
SHEET    9   A 9 LYS A  12  CYS A  16 -1  N  LYS A  12   O  LEU A  44           
SHEET    1   B 2 TYR A  64  MET A  67  0                                        
SHEET    2   B 2 LEU A  73  SER A  76 -1  O  SER A  76   N  TYR A  64           
SHEET    1   C 4 VAL B  31  THR B  34  0                                        
SHEET    2   C 4 HIS B  21  ILE B  25 -1  N  ARG B  24   O  ASP B  32           
SHEET    3   C 4 LEU B  13  CYS B  16 -1  N  CYS B  16   O  HIS B  21           
SHEET    4   C 4 PHE B 132  LEU B 135 -1  O  LEU B 135   N  LEU B  13           
SHEET    1   D 4 LEU B  44  ALA B  48  0                                        
SHEET    2   D 4 GLU B  53  SER B  58 -1  O  LYS B  57   N  GLN B  45           
SHEET    3   D 4 PHE B  85  GLU B  90 -1  O  PHE B  85   N  VAL B  54           
SHEET    4   D 4 TYR B  94  SER B  99 -1  O  ILE B  98   N  LEU B  86           
SHEET    1   E 2 TYR B  64  MET B  67  0                                        
SHEET    2   E 2 LEU B  73  SER B  76 -1  O  SER B  76   N  TYR B  64           
SHEET    1   F 4 VAL C  31  THR C  34  0                                        
SHEET    2   F 4 HIS C  21  ILE C  25 -1  N  ARG C  24   O  ASP C  32           
SHEET    3   F 4 LYS C  12  CYS C  16 -1  N  CYS C  16   O  HIS C  21           
SHEET    4   F 4 PHE C 132  PRO C 136 -1  O  LEU C 135   N  LEU C  13           
SHEET    1   G 4 LEU C  44  ALA C  48  0                                        
SHEET    2   G 4 GLU C  53  SER C  58 -1  O  LYS C  57   N  GLN C  45           
SHEET    3   G 4 PHE C  85  GLU C  90 -1  O  PHE C  85   N  VAL C  54           
SHEET    4   G 4 TYR C  94  SER C  99 -1  O  THR C  96   N  ARG C  88           
SHEET    1   H 2 TYR C  64  MET C  67  0                                        
SHEET    2   H 2 LEU C  73  SER C  76 -1  O  SER C  76   N  TYR C  64           
SHEET    1   I 4 VAL D  31  THR D  34  0                                        
SHEET    2   I 4 HIS D  21  ILE D  25 -1  N  ARG D  24   O  ASP D  32           
SHEET    3   I 4 LYS D  12  CYS D  16 -1  N  CYS D  16   O  HIS D  21           
SHEET    4   I 4 PHE D 132  PRO D 136 -1  O  LEU D 135   N  LEU D  13           
SHEET    1   J 4 LEU D  44  ALA D  48  0                                        
SHEET    2   J 4 GLU D  53  SER D  58 -1  O  LYS D  57   N  GLN D  45           
SHEET    3   J 4 PHE D  85  GLU D  90 -1  O  PHE D  85   N  VAL D  54           
SHEET    4   J 4 TYR D  94  SER D  99 -1  O  THR D  96   N  ARG D  88           
SHEET    1   K 2 TYR D  64  MET D  67  0                                        
SHEET    2   K 2 LEU D  73  SER D  76 -1  O  SER D  76   N  TYR D  64           
CRYST1   60.600  108.330   60.920  90.00 104.97  90.00 P 1 21 1      8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016502  0.000000  0.004412        0.00000                         
SCALE2      0.000000  0.009231  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.016992        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system