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Database: PDB
Entry: 2PON
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HEADER    APOPTOSIS INHIBITOR                     27-APR-07   2PON              
TITLE     SOLUTION STRUCTURE OF THE BCL-XL/BECLIN-1 COMPLEX                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: BECLIN-1;                                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: COILED-COIL MYOSIN-LIKE BCL2-INTERACTING PROTEIN;           
COMPND   5 PROTEIN GT197;                                                       
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: APOPTOSIS REGULATOR BCL-X;                                 
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: DELETION OF 45-84;                                         
COMPND  11 SYNONYM: BCL-2-LIKE 1 PROTEIN;                                       
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: BECN1, GT197;                                                  
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PET32A;                                   
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 GENE: BCL2L1, BCL2L, BCLX;                                           
SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  16 EXPRESSION_SYSTEM_PLASMID: PET32A                                    
KEYWDS    APOPTOSIS; AUTOPHAGY; BCL-2 FAMILY PROTEINS; BECLIN-1,                
KEYWDS   2 APOPTOSIS INHIBITOR                                                  
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
MDLTYP    MINIMIZED AVERAGE                                                     
AUTHOR    W.FENG,S.HUANG,H.WU,M.ZHANG                                           
REVDAT   2   24-FEB-09 2PON    1       VERSN                                    
REVDAT   1   04-SEP-07 2PON    0                                                
JRNL        AUTH   W.FENG,S.HUANG,H.WU,M.ZHANG                                  
JRNL        TITL   MOLECULAR BASIS OF BCL-XL'S TARGET RECOGNITION               
JRNL        TITL 2 VERSATILITY REVEALED BY THE STRUCTURE OF BCL-XL IN           
JRNL        TITL 3 COMPLEX WITH THE BH3 DOMAIN OF BECLIN-1.                     
JRNL        REF    J.MOL.BIOL.                   V. 372   223 2007              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   17659302                                                     
JRNL        DOI    10.1016/J.JMB.2007.06.069                                    
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER, A.T.                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2PON COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 16-MAY-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB042611.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 303                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 50MM POTASSIUM PHOSPHATE           
REMARK 210  PRESSURE                       : 1ATM                               
REMARK 210  SAMPLE CONTENTS                : 1.5MM UNLABELLED BCL-XL/           
REMARK 210                                   BECLIN-1 COMPLEX IN 99.9% D2O;     
REMARK 210                                   50MM POTASSIUM PHOSPHATE;          
REMARK 210                                   1.5MM UNIFORMLY 15N LABELLED       
REMARK 210                                   BCL-XL/BECLIN-1 COMPLEX IN 90%     
REMARK 210                                   H2O/10% D2O; 50MM POTASSIUM        
REMARK 210                                   PHOSPHATE; 1.5MM UNIFORMLY         
REMARK 210                                   15N/13C LABELLED BCL-XL/BECLIN     
REMARK 210                                   -1 COMPLEX IN 99.9% D2O; 50MM      
REMARK 210                                   POTASSIUM PHOSPHATE; 1.5MM 80%     
REMARK 210                                   DEUTERATED, UNIFORMLY 15N/13C      
REMARK 210                                   LABELLED BCL-XL/BECLIN-1           
REMARK 210                                   COMPLEX IN 90% H2O/10% D2O;        
REMARK 210                                   50MM POTASSIUM PHOSPHATE;          
REMARK 210                                   1.5MM 100% DEUTERATED,             
REMARK 210                                   UNIFORMLY 15N LABELLED BCL-XL/     
REMARK 210                                   BECLIN-1 COMPLEX IN 90% H2O/       
REMARK 210                                   10% D2O; 50MM POTASSIUM            
REMARK 210                                   PHOSPHATE                          
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D NOESY, 3D_15N-SEPARATED_        
REMARK 210                                   NOESY, 3D_13C-SEPARATED_NOESY,     
REMARK 210                                   HNCO, HNCA, HN(CO)CA, HNCACB       
REMARK 210                                   AND CBCA(CO)NH                     
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 750 MHZ                   
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : CNS 1.1                            
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 200                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 18                  
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 THR A  23      -53.47   -125.17                                   
REMARK 500  1 ASP A  39      -80.18   -126.52                                   
REMARK 500  1 ILE A  40      161.55     58.82                                   
REMARK 500  1 MET A  41      -71.70    -95.13                                   
REMARK 500  1 SER B  58      107.20     61.76                                   
REMARK 500  1 GLN B  59      -46.53   -130.76                                   
REMARK 500  1 SER B  60       70.22   -155.23                                   
REMARK 500  1 PHE B  83       42.46   -155.92                                   
REMARK 500  1 ASP B  85      -21.94     74.46                                   
REMARK 500  1 VAL B  86       20.03     47.43                                   
REMARK 500  1 GLU B  88      -74.76   -177.94                                   
REMARK 500  1 ASN B  89      123.33    173.00                                   
REMARK 500  1 THR B  91      -64.49   -138.44                                   
REMARK 500  1 GLU B  92      128.25     60.80                                   
REMARK 500  1 PRO B  94      -74.97    -64.49                                   
REMARK 500  1 ALA B 120      -64.50    -99.05                                   
REMARK 500  1 SER B 122      175.67     52.85                                   
REMARK 500  1 ASP B 123     -177.29    -53.92                                   
REMARK 500  1 SER B 126       54.38    -97.47                                   
REMARK 500  1 GLN B 127      -53.32   -165.01                                   
REMARK 500  1 LEU B 128     -172.41    -59.85                                   
REMARK 500  1 HIS B 129       92.68   -165.74                                   
REMARK 500  1 THR B 134       13.71   -142.73                                   
REMARK 500  1 ASN B 152      158.75    169.18                                   
REMARK 500  1 LYS B 173      -70.27    -78.80                                   
REMARK 500  1 GLU B 174       43.35    168.16                                   
REMARK 500  2 ILE A  40      159.13     60.11                                   
REMARK 500  2 MET A  41      -45.78   -142.15                                   
REMARK 500  2 SER B  58       87.48     60.82                                   
REMARK 500  2 GLN B  59      -68.17   -109.04                                   
REMARK 500  2 SER B  60       63.77   -171.54                                   
REMARK 500  2 PHE B  83       37.77   -143.49                                   
REMARK 500  2 GLU B  88      -50.91    178.43                                   
REMARK 500  2 ASN B  89      153.82     68.25                                   
REMARK 500  2 PRO B  94      -70.68    -65.03                                   
REMARK 500  2 GLU B  95      146.38   -177.04                                   
REMARK 500  2 PHE B 121      -75.39   -123.89                                   
REMARK 500  2 SER B 122      171.74    175.31                                   
REMARK 500  2 SER B 126       36.30    -97.16                                   
REMARK 500  2 GLN B 127       22.03   -148.23                                   
REMARK 500  2 HIS B 129      109.82   -169.15                                   
REMARK 500  2 PRO B 132       80.82    -56.64                                   
REMARK 500  2 ASN B 152      160.92    161.85                                   
REMARK 500  2 LYS B 173      -71.21    -79.39                                   
REMARK 500  2 GLU B 174       42.67    168.82                                   
REMARK 500  3 THR A  23      -67.99     68.72                                   
REMARK 500  3 ASP A  39      -71.06    -96.30                                   
REMARK 500  3 ILE A  40      157.21     60.16                                   
REMARK 500  3 MET A  41      -31.35   -137.79                                   
REMARK 500  3 SER B  58       87.72     60.32                                   
REMARK 500  3 GLN B  59      -66.71   -100.64                                   
REMARK 500  3 SER B  84      -62.88   -138.41                                   
REMARK 500  3 ASN B  89     -176.35     83.76                                   
REMARK 500  3 ARG B  90       46.16    -94.75                                   
REMARK 500  3 THR B  91      -70.73   -138.47                                   
REMARK 500  3 GLU B  92      128.88     62.63                                   
REMARK 500  3 PHE B 121      -75.91   -133.86                                   
REMARK 500  3 SER B 122     -166.47   -178.25                                   
REMARK 500  3 LEU B 124      -63.79    -96.20                                   
REMARK 500  3 HIS B 129       72.09   -161.28                                   
REMARK 500  3 PRO B 132       81.23    -53.37                                   
REMARK 500  3 ASN B 152      163.09    166.68                                   
REMARK 500  3 LYS B 173      -70.79    -73.64                                   
REMARK 500  3 GLU B 174       43.20    161.87                                   
REMARK 500  4 THR A  23      -64.70   -122.90                                   
REMARK 500  4 MET A  41      -66.33     64.84                                   
REMARK 500  4 SER B  58       49.99   -101.16                                   
REMARK 500  4 SER B  60       95.07   -178.28                                   
REMARK 500  4 PHE B  83       48.34   -144.78                                   
REMARK 500  4 GLU B  87       76.93     70.94                                   
REMARK 500  4 ASN B  89     -178.00     81.52                                   
REMARK 500  4 THR B  91      -65.03   -122.57                                   
REMARK 500  4 GLU B  92      137.61     68.16                                   
REMARK 500  4 ALA B 120      -65.08   -101.81                                   
REMARK 500  4 SER B 122     -168.69     50.79                                   
REMARK 500  4 ASP B 123      174.94    -55.14                                   
REMARK 500  4 LEU B 128      178.85    -53.73                                   
REMARK 500  4 HIS B 129       34.21   -170.55                                   
REMARK 500  4 PRO B 132       64.11    -69.36                                   
REMARK 500  4 ASN B 152      163.09    163.23                                   
REMARK 500  4 GLU B 174       44.06    163.11                                   
REMARK 500  4 MET B 175       62.64   -150.89                                   
REMARK 500  5 THR A  23      -67.93   -125.74                                   
REMARK 500  5 PHE A  38       50.98   -107.31                                   
REMARK 500  5 ASP A  39      -71.31   -107.45                                   
REMARK 500  5 ILE A  40      -72.72     66.38                                   
REMARK 500  5 SER B  58       76.28     65.22                                   
REMARK 500  5 SER B  60       85.22   -172.30                                   
REMARK 500  5 PHE B  83       29.35   -149.16                                   
REMARK 500  5 ASP B  85      -59.39     72.61                                   
REMARK 500  5 VAL B  86      -26.27     86.77                                   
REMARK 500  5 GLU B  88      -80.03   -166.30                                   
REMARK 500  5 ALA B  93      135.62     61.83                                   
REMARK 500  5 GLU B  95      124.54     71.24                                   
REMARK 500  5 SER B 122     -172.80     52.42                                   
REMARK 500  5 LEU B 128      166.50    -45.53                                   
REMARK 500  5 HIS B 129       65.73   -162.37                                   
REMARK 500  5 PRO B 132       62.70    -69.59                                   
REMARK 500  5 ASN B 152      166.17    162.08                                   
REMARK 500  5 LYS B 173      -71.66    -71.43                                   
REMARK 500  5 GLU B 174       42.16    165.47                                   
REMARK 500  5 MET B 175       65.09   -157.07                                   
REMARK 500  6 THR A  23      -51.95   -128.68                                   
REMARK 500  6 ILE A  40      162.80     59.33                                   
REMARK 500  6 SER B  58      152.50     61.86                                   
REMARK 500  6 GLN B  59      -34.12   -175.82                                   
REMARK 500  6 SER B  60       41.42   -178.66                                   
REMARK 500  6 PHE B  83       58.55   -144.97                                   
REMARK 500  6 ASN B  89       56.06    -98.46                                   
REMARK 500  6 GLU B  92      130.32     63.82                                   
REMARK 500  6 ALA B  93      151.17    -46.84                                   
REMARK 500  6 PRO B  94      -72.01    -74.94                                   
REMARK 500  6 GLU B  95      148.40   -175.69                                   
REMARK 500  6 ALA B 120      -61.40    -97.10                                   
REMARK 500  6 SER B 122      110.84     73.15                                   
REMARK 500  6 ASP B 123      141.23     62.56                                   
REMARK 500  6 GLN B 127      -55.32   -152.09                                   
REMARK 500  6 HIS B 129       60.69   -152.37                                   
REMARK 500  6 PRO B 132       69.95    -60.00                                   
REMARK 500  6 ASN B 152      160.65    165.81                                   
REMARK 500  6 LYS B 173      -72.38    -83.00                                   
REMARK 500  6 GLU B 174       46.68    167.17                                   
REMARK 500  6 MET B 175       49.78   -145.20                                   
REMARK 500  7 THR A  23      -66.22   -104.96                                   
REMARK 500  7 ILE A  40      160.54     58.37                                   
REMARK 500  7 MET A  41      -37.25   -160.51                                   
REMARK 500  7 SER A  42     -163.22    -62.14                                   
REMARK 500  7 SER B  58       71.92     67.63                                   
REMARK 500  7 GLN B  59      -76.77    -68.00                                   
REMARK 500  7 PHE B  83       40.35   -142.06                                   
REMARK 500  7 GLU B  87       65.49     64.97                                   
REMARK 500  7 GLU B  88       28.35     44.80                                   
REMARK 500  7 ASN B  89     -162.69   -119.56                                   
REMARK 500  7 ARG B  90      -23.64     91.32                                   
REMARK 500  7 THR B  91      -67.75    176.83                                   
REMARK 500  7 GLU B  92      128.71     59.84                                   
REMARK 500  7 PRO B  94      -73.22    -60.56                                   
REMARK 500  7 GLU B  95      119.00   -163.41                                   
REMARK 500  7 THR B  97     -169.85    -67.27                                   
REMARK 500  7 PHE B 121      -75.88   -130.93                                   
REMARK 500  7 SER B 122      174.46    169.87                                   
REMARK 500  7 SER B 126       41.44    -92.89                                   
REMARK 500  7 GLN B 127      -63.51   -150.40                                   
REMARK 500  7 LEU B 128     -176.82    -63.41                                   
REMARK 500  7 HIS B 129       91.25   -161.81                                   
REMARK 500  7 PRO B 132       69.86    -65.02                                   
REMARK 500  7 ASN B 152      165.39    164.96                                   
REMARK 500  8 THR A  23      -72.19     65.49                                   
REMARK 500  8 ILE A  40      -67.64     67.61                                   
REMARK 500  8 MET A  41      -76.16     68.95                                   
REMARK 500  8 GLN B  59       47.98    -93.37                                   
REMARK 500  8 PHE B  83       33.08   -140.68                                   
REMARK 500  8 VAL B  86      -28.13     89.52                                   
REMARK 500  8 GLU B  88      -39.30   -150.46                                   
REMARK 500  8 ARG B  90       71.88     54.85                                   
REMARK 500  8 THR B  91       33.53   -176.70                                   
REMARK 500  8 ALA B  93      142.56     59.24                                   
REMARK 500  8 PRO B  94      -71.56    -66.24                                   
REMARK 500  8 GLU B  95      134.41   -175.08                                   
REMARK 500  8 PHE B 121      -79.58   -136.05                                   
REMARK 500  8 SER B 122     -166.68    171.76                                   
REMARK 500  8 ASP B 123      179.58    -57.13                                   
REMARK 500  8 SER B 126       48.79    -88.98                                   
REMARK 500  8 GLN B 127      -46.58   -147.46                                   
REMARK 500  8 HIS B 129       74.64   -157.06                                   
REMARK 500  8 PRO B 132       75.65    -58.26                                   
REMARK 500  8 ASN B 152      160.46    164.28                                   
REMARK 500  8 LYS B 173      -71.09    -78.20                                   
REMARK 500  8 GLU B 174       43.81    169.02                                   
REMARK 500  8 MET B 175       54.09   -144.50                                   
REMARK 500  9 THR A  23      -64.89   -101.19                                   
REMARK 500  9 ILE A  40      -78.33     64.62                                   
REMARK 500  9 SER B  58       32.82   -168.34                                   
REMARK 500  9 GLN B  59      -37.84    -38.94                                   
REMARK 500  9 SER B  60      101.69    172.20                                   
REMARK 500  9 VAL B  86      -33.10     89.67                                   
REMARK 500  9 GLU B  88      -73.33    178.19                                   
REMARK 500  9 ASN B  89     -177.51   -174.05                                   
REMARK 500  9 GLU B  92      124.36     61.14                                   
REMARK 500  9 ALA B  93      143.80     61.35                                   
REMARK 500  9 GLU B  95      130.64     71.47                                   
REMARK 500  9 ALA B 120      -66.97    -92.98                                   
REMARK 500  9 SER B 122      178.22     51.49                                   
REMARK 500  9 ASP B 123      174.86    -57.01                                   
REMARK 500  9 SER B 126       49.61    -91.36                                   
REMARK 500  9 GLN B 127      -46.16   -160.79                                   
REMARK 500  9 LEU B 128     -167.80    -73.90                                   
REMARK 500  9 HIS B 129      111.32   -174.96                                   
REMARK 500  9 THR B 134       15.66   -141.72                                   
REMARK 500  9 ASN B 152      163.47    176.26                                   
REMARK 500  9 LYS B 173      -72.43    -82.59                                   
REMARK 500  9 GLU B 174       46.86    168.80                                   
REMARK 500  9 MET B 175       51.87   -147.82                                   
REMARK 500 10 THR A  23      -64.01   -106.79                                   
REMARK 500 10 ASP A  39      -73.81    -84.84                                   
REMARK 500 10 ILE A  40      156.85     60.10                                   
REMARK 500 10 MET A  41       29.89   -155.60                                   
REMARK 500 10 GLN B  59      -71.92   -133.41                                   
REMARK 500 10 SER B  60       42.88   -166.38                                   
REMARK 500 10 ASP B  85      -61.98     72.58                                   
REMARK 500 10 VAL B  86      -30.49     88.63                                   
REMARK 500 10 GLU B  88      -69.15   -147.06                                   
REMARK 500 10 GLU B  92      122.31     62.53                                   
REMARK 500 10 ALA B  93      149.63     60.83                                   
REMARK 500 10 PRO B  94      -73.36    -78.07                                   
REMARK 500 10 ALA B 120      -71.99    -99.41                                   
REMARK 500 10 SER B 122     -169.28     47.34                                   
REMARK 500 10 ASP B 123     -176.41    -56.89                                   
REMARK 500 10 HIS B 129       76.54   -170.38                                   
REMARK 500 10 PRO B 132       65.04    -66.97                                   
REMARK 500 10 ASN B 152      161.31    165.83                                   
REMARK 500 10 GLU B 174       41.60    162.72                                   
REMARK 500 11 ILE A  40      162.19     64.64                                   
REMARK 500 11 MET A  41       85.30    -61.55                                   
REMARK 500 11 SER B  58       89.21    -59.96                                   
REMARK 500 11 SER B  60       73.68   -176.92                                   
REMARK 500 11 PHE B  83       45.38   -143.78                                   
REMARK 500 11 VAL B  86      -48.65   -144.90                                   
REMARK 500 11 GLU B  87       25.42     42.10                                   
REMARK 500 11 GLU B  88      -50.25    152.64                                   
REMARK 500 11 ASN B  89      176.61    -46.41                                   
REMARK 500 11 ARG B  90      -42.86     84.57                                   
REMARK 500 11 THR B  91      -70.77   -161.82                                   
REMARK 500 11 GLU B  92      128.87     62.89                                   
REMARK 500 11 THR B  97     -160.32   -121.51                                   
REMARK 500 11 ALA B 120      -68.02    -97.58                                   
REMARK 500 11 SER B 122     -169.63     47.53                                   
REMARK 500 11 GLN B 127       54.19    177.58                                   
REMARK 500 11 LEU B 128      -61.86   -128.24                                   
REMARK 500 11 HIS B 129       34.69     75.58                                   
REMARK 500 11 ASN B 152      164.08    164.85                                   
REMARK 500 11 LYS B 173      -71.80    -69.81                                   
REMARK 500 11 GLU B 174       36.21    168.18                                   
REMARK 500 11 MET B 175       49.69   -156.33                                   
REMARK 500 12 THR A  23      -67.97   -125.33                                   
REMARK 500 12 ILE A  40      -79.00     64.23                                   
REMARK 500 12 MET A  41       71.10     45.38                                   
REMARK 500 12 SER A  42      -45.13   -134.49                                   
REMARK 500 12 SER B  58       72.85     62.95                                   
REMARK 500 12 SER B  60       77.08   -167.74                                   
REMARK 500 12 GLU B  87       76.07     66.32                                   
REMARK 500 12 ASN B  89      152.92     68.00                                   
REMARK 500 12 THR B  91      -59.34     68.67                                   
REMARK 500 12 GLU B  92      161.42     58.89                                   
REMARK 500 12 PRO B  94      -70.84    -62.89                                   
REMARK 500 12 GLU B  95      146.39   -175.96                                   
REMARK 500 12 ALA B 120      -69.26    -98.40                                   
REMARK 500 12 SER B 122      174.47     53.90                                   
REMARK 500 12 ASP B 123      162.82    -49.31                                   
REMARK 500 12 GLN B 127      -44.19   -174.62                                   
REMARK 500 12 HIS B 129      116.42   -160.28                                   
REMARK 500 12 ASN B 152      163.68    166.18                                   
REMARK 500 12 LYS B 173      -71.59    -71.63                                   
REMARK 500 12 GLU B 174       41.46    165.86                                   
REMARK 500 12 MET B 175       66.26   -154.27                                   
REMARK 500 13 THR A  23      -51.41   -137.37                                   
REMARK 500 13 ILE A  40      -82.62     60.77                                   
REMARK 500 13 MET A  41       71.25     46.81                                   
REMARK 500 13 GLN B  59      -73.93   -178.62                                   
REMARK 500 13 SER B  60       41.44   -177.80                                   
REMARK 500 13 PHE B  83       34.24   -143.92                                   
REMARK 500 13 GLU B  87       32.20     39.64                                   
REMARK 500 13 GLU B  92      118.64     59.11                                   
REMARK 500 13 ALA B  93      121.58     56.19                                   
REMARK 500 13 GLU B  95      129.79     75.09                                   
REMARK 500 13 SER B 122       80.25     75.11                                   
REMARK 500 13 ASP B 123      155.36     59.93                                   
REMARK 500 13 HIS B 129       47.78   -164.47                                   
REMARK 500 13 THR B 134       11.87   -142.05                                   
REMARK 500 13 ASN B 152      166.35    164.38                                   
REMARK 500 13 LYS B 173      -73.23    -70.12                                   
REMARK 500 13 GLU B 174       36.05    170.21                                   
REMARK 500 13 MET B 175       54.85   -161.42                                   
REMARK 500 14 ASP A  39      -66.91    -96.19                                   
REMARK 500 14 ILE A  40      -73.07     67.09                                   
REMARK 500 14 SER B  58       76.62     54.59                                   
REMARK 500 14 SER B  60       61.53     66.56                                   
REMARK 500 14 ASN B  89     -171.49     60.81                                   
REMARK 500 14 THR B  91      -54.72   -128.71                                   
REMARK 500 14 GLU B  92      156.13     59.19                                   
REMARK 500 14 PRO B  94      -70.51    -56.61                                   
REMARK 500 14 GLU B  95      149.47   -177.27                                   
REMARK 500 14 ALA B 120      -70.11   -102.93                                   
REMARK 500 14 SER B 122      172.40     55.31                                   
REMARK 500 14 ASP B 123      174.03    -53.17                                   
REMARK 500 14 LEU B 128      178.39    -52.24                                   
REMARK 500 14 HIS B 129       34.97   -172.70                                   
REMARK 500 14 PRO B 132       81.12    -59.98                                   
REMARK 500 14 ASN B 152      167.17    176.37                                   
REMARK 500 14 GLU B 174       62.84     63.08                                   
REMARK 500 15 THR A  23      -68.21     67.54                                   
REMARK 500 15 MET A  41      -46.82   -136.97                                   
REMARK 500 15 SER A  42      -74.54     65.51                                   
REMARK 500 15 SER B  58      112.60     60.46                                   
REMARK 500 15 GLU B  88      -50.65   -179.24                                   
REMARK 500 15 ASN B  89      168.78     67.72                                   
REMARK 500 15 ARG B  90      -79.73    -42.47                                   
REMARK 500 15 ALA B 120      -60.06   -106.09                                   
REMARK 500 15 SER B 122     -167.42     47.49                                   
REMARK 500 15 HIS B 129       61.24   -168.29                                   
REMARK 500 15 PRO B 132       78.89    -55.68                                   
REMARK 500 15 ASN B 152      162.67    166.07                                   
REMARK 500 15 LYS B 173      -71.72    -83.39                                   
REMARK 500 15 GLU B 174       42.44    170.39                                   
REMARK 500 15 MET B 175       55.31   -144.36                                   
REMARK 500 16 ILE A  40      -78.89     65.06                                   
REMARK 500 16 MET A  41      -73.32     65.17                                   
REMARK 500 16 SER A  42     -168.06     52.56                                   
REMARK 500 16 GLN B  59       40.16    -96.58                                   
REMARK 500 16 SER B  60       90.12     63.06                                   
REMARK 500 16 PHE B  83       57.96   -143.07                                   
REMARK 500 16 GLU B  92      129.05     63.10                                   
REMARK 500 16 ALA B  93      155.15    -44.52                                   
REMARK 500 16 PRO B  94      -70.97    -74.71                                   
REMARK 500 16 PHE B 121      -82.34   -102.15                                   
REMARK 500 16 SER B 122     -168.93   -178.88                                   
REMARK 500 16 ASP B 123     -175.74    -59.04                                   
REMARK 500 16 HIS B 129      105.70   -161.31                                   
REMARK 500 16 PRO B 132       64.22    -65.22                                   
REMARK 500 16 ASN B 152      162.70    165.71                                   
REMARK 500 16 LYS B 173      -73.46    -84.76                                   
REMARK 500 16 GLU B 174       46.82    169.34                                   
REMARK 500 16 MET B 175       49.58   -146.47                                   
REMARK 500 17 THR A  23      -66.19   -122.96                                   
REMARK 500 17 ILE A  40      159.28     58.75                                   
REMARK 500 17 MET A  41      -35.72   -130.29                                   
REMARK 500 17 SER B  58       65.22     60.17                                   
REMARK 500 17 SER B  60       46.86   -178.68                                   
REMARK 500 17 ASN B  89      166.57     86.19                                   
REMARK 500 17 THR B  91      -53.87   -124.49                                   
REMARK 500 17 GLU B  92      133.48     63.25                                   
REMARK 500 17 PHE B 121      -75.73   -125.19                                   
REMARK 500 17 SER B 122     -178.70    176.85                                   
REMARK 500 17 HIS B 129       83.29   -153.26                                   
REMARK 500 17 THR B 134       13.09   -140.95                                   
REMARK 500 17 ASN B 152      163.26    165.18                                   
REMARK 500 18 THR A  23      -64.69   -128.26                                   
REMARK 500 18 ILE A  40      158.61     59.54                                   
REMARK 500 18 SER B  58       47.17     72.34                                   
REMARK 500 18 GLN B  59      -75.12    -62.58                                   
REMARK 500 18 PHE B  83       43.49   -141.00                                   
REMARK 500 18 SER B  84      -46.66   -132.66                                   
REMARK 500 18 VAL B  86      -50.14   -137.86                                   
REMARK 500 18 GLU B  88      -49.05   -170.72                                   
REMARK 500 18 ASN B  89       83.59     53.90                                   
REMARK 500 18 ARG B  90      -75.70     60.34                                   
REMARK 500 18 ALA B  93       97.02     42.23                                   
REMARK 500 18 GLU B  95      125.84     70.83                                   
REMARK 500 18 PHE B 121      -79.06   -133.98                                   
REMARK 500 18 SER B 122      178.78    172.60                                   
REMARK 500 18 GLN B 127      -44.84   -136.22                                   
REMARK 500 18 HIS B 129       82.94   -164.47                                   
REMARK 500 18 PRO B 132       75.33    -59.42                                   
REMARK 500 18 ASN B 152      163.06    167.45                                   
REMARK 500 18 LYS B 173      -71.43    -81.74                                   
REMARK 500 18 GLU B 174       42.02    167.07                                   
REMARK 500 19 THR A  23      -72.85   -106.31                                   
REMARK 500 19 ILE A  40      160.98     59.67                                   
REMARK 500 19 SER A  42     -160.06     60.09                                   
REMARK 500 19 SER B  58       64.93    179.53                                   
REMARK 500 19 SER B  60       74.27     47.05                                   
REMARK 500 19 GLU B  88       20.28     46.63                                   
REMARK 500 19 ASN B  89      108.42    162.81                                   
REMARK 500 19 THR B  91      -48.36   -147.58                                   
REMARK 500 19 GLU B  92     -166.90     44.71                                   
REMARK 500 19 THR B  97      177.49    -53.45                                   
REMARK 500 19 PHE B 121      -86.60   -130.55                                   
REMARK 500 19 SER B 122       86.42    168.64                                   
REMARK 500 19 ASP B 123      175.92     50.96                                   
REMARK 500 19 GLN B 127      -57.25   -152.97                                   
REMARK 500 19 LEU B 128     -173.10    -63.24                                   
REMARK 500 19 HIS B 129       79.57   -165.11                                   
REMARK 500 19 PRO B 132       66.51    -65.57                                   
REMARK 500 19 ASN B 152      166.53    167.79                                   
REMARK 500 19 GLU B 174       42.84    163.51                                   
REMARK 500 20 THR A  23      -68.79   -104.45                                   
REMARK 500 20 ILE A  40      -77.36     65.37                                   
REMARK 500 20 MET A  41      -80.92     62.23                                   
REMARK 500 20 SER B  58       87.75     60.69                                   
REMARK 500 20 GLN B  59      -64.16   -120.17                                   
REMARK 500 20 SER B  60       39.78   -177.83                                   
REMARK 500 20 PHE B  83       45.37   -141.70                                   
REMARK 500 20 GLU B  87       69.64     65.06                                   
REMARK 500 20 GLU B  88       28.07     43.21                                   
REMARK 500 20 ASN B  89     -172.34     73.69                                   
REMARK 500 20 THR B  91       25.22   -157.82                                   
REMARK 500 20 ALA B  93      128.12     57.68                                   
REMARK 500 20 PRO B  94      -72.86    -57.81                                   
REMARK 500 20 THR B  97     -169.55    -65.30                                   
REMARK 500 20 ALA B 120      -61.24    -96.38                                   
REMARK 500 20 PHE B 121      -78.19    -95.45                                   
REMARK 500 20 SER B 122      178.52    177.60                                   
REMARK 500 20 SER B 126       49.00    -90.01                                   
REMARK 500 20 GLN B 127       28.71   -166.90                                   
REMARK 500 20 PRO B 132       64.38    -68.02                                   
REMARK 500 20 ASN B 152      164.84    166.36                                   
REMARK 500 20 LYS B 173      -71.54    -85.45                                   
REMARK 500 20 GLU B 174       44.15    168.32                                   
REMARK 500 20 MET B 175       50.20   -144.94                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  2PON A   21    43  UNP    Q14457   BCN1_HUMAN       1     23             
DBREF  2PON B   57   100  UNP    Q07817   BCLX_HUMAN       1     44             
DBREF  2PON B  101   212  UNP    Q07817   BCLX_HUMAN      85    196             
SEQRES   1 A   23  GLY GLY THR MET GLU ASN LEU SER ARG ARG LEU LYS VAL          
SEQRES   2 A   23  THR GLY ASP LEU PHE ASP ILE MET SER GLY                      
SEQRES   1 B  156  MET SER GLN SER ASN ARG GLU LEU VAL VAL ASP PHE LEU          
SEQRES   2 B  156  SER TYR LYS LEU SER GLN LYS GLY TYR SER TRP SER GLN          
SEQRES   3 B  156  PHE SER ASP VAL GLU GLU ASN ARG THR GLU ALA PRO GLU          
SEQRES   4 B  156  GLY THR GLU SER GLU ALA VAL LYS GLN ALA LEU ARG GLU          
SEQRES   5 B  156  ALA GLY ASP GLU PHE GLU LEU ARG TYR ARG ARG ALA PHE          
SEQRES   6 B  156  SER ASP LEU THR SER GLN LEU HIS ILE THR PRO GLY THR          
SEQRES   7 B  156  ALA TYR GLN SER PHE GLU GLN VAL VAL ASN GLU LEU PHE          
SEQRES   8 B  156  ARG ASP GLY VAL ASN TRP GLY ARG ILE VAL ALA PHE PHE          
SEQRES   9 B  156  SER PHE GLY GLY ALA LEU CYS VAL GLU SER VAL ASP LYS          
SEQRES  10 B  156  GLU MET GLN VAL LEU VAL SER ARG ILE ALA ALA TRP MET          
SEQRES  11 B  156  ALA THR TYR LEU ASN ASP HIS LEU GLU PRO TRP ILE GLN          
SEQRES  12 B  156  GLU ASN GLY GLY TRP ASP THR PHE VAL GLU LEU TYR GLY          
HELIX    1   1 THR A   23  PHE A   38  1                                  16    
HELIX    2   2 SER B   60  GLY B   77  1                                  18    
HELIX    3   3 SER B   84  GLU B   88  5                                   5    
HELIX    4   4 THR B   97  PHE B  121  1                                  25    
HELIX    5   5 THR B  134  ASP B  149  1                                  16    
HELIX    6   6 ASN B  152  GLU B  174  1                                  23    
HELIX    7   7 VAL B  177  HIS B  193  1                                  17    
HELIX    8   8 LEU B  194  ASN B  201  1                                   8    
HELIX    9   9 GLY B  203  GLY B  212  1                                  10    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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