HEADER PLANT PROTEIN 15-AUG-07 2QYS
TITLE STRUCTURE OF EUGENOL SYNTHASE FROM OCIMUM BASILICUM
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: EUGENOL SYNTHASE 1;
COMPND 3 CHAIN: A, B;
COMPND 4 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: OCIMUM BASILICUM;
SOURCE 3 ORGANISM_COMMON: SWEET BASIL;
SOURCE 4 ORGANISM_TAXID: 39350;
SOURCE 5 GENE: EGS1;
SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);
SOURCE 7 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);
SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PHIS8
KEYWDS EUGENOL, PHENYLPROPENE, PIP REDUCTASE, SHORT-CHAIN
KEYWDS 2 DEHYDROGENASE/REDUCTASE, PLANT PROTEIN
EXPDTA X-RAY DIFFRACTION
AUTHOR G.V.LOUIE,J.P.NOEL,M.E.BOWMAN
REVDAT 3 25-OCT-17 2QYS 1 REMARK
REVDAT 2 24-FEB-09 2QYS 1 VERSN
REVDAT 1 15-JAN-08 2QYS 0
JRNL AUTH G.V.LOUIE,T.J.BAIGA,M.E.BOWMAN,T.KOEDUKA,J.H.TAYLOR,
JRNL AUTH 2 S.M.SPASSOVA,E.PICHERSKY,J.P.NOEL
JRNL TITL STRUCTURE AND REACTION MECHANISM OF BASIL EUGENOL SYNTHASE
JRNL REF PLOS ONE V. 2 E993 2007
JRNL REFN ESSN 1932-6203
JRNL PMID 17912370
JRNL DOI 10.1371/JOURNAL.PONE.0000993
REMARK 2
REMARK 2 RESOLUTION. 1.80 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : CNS
REMARK 3 AUTHORS : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-
REMARK 3 : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,
REMARK 3 : READ,RICE,SIMONSON,WARREN
REMARK 3
REMARK 3 REFINEMENT TARGET : ENGH & HUBER
REMARK 3
REMARK 3 DATA USED IN REFINEMENT.
REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 1.80
REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 41.76
REMARK 3 DATA CUTOFF (SIGMA(F)) : 0.000
REMARK 3 DATA CUTOFF HIGH (ABS(F)) : NULL
REMARK 3 DATA CUTOFF LOW (ABS(F)) : NULL
REMARK 3 COMPLETENESS (WORKING+TEST) (%) : 95.4
REMARK 3 NUMBER OF REFLECTIONS : 60262
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT.
REMARK 3 CROSS-VALIDATION METHOD : THROUGHOUT
REMARK 3 FREE R VALUE TEST SET SELECTION : RANDOM
REMARK 3 R VALUE (WORKING SET) : 0.224
REMARK 3 FREE R VALUE : 0.242
REMARK 3 FREE R VALUE TEST SET SIZE (%) : 4.800
REMARK 3 FREE R VALUE TEST SET COUNT : 3039
REMARK 3 ESTIMATED ERROR OF FREE R VALUE : NULL
REMARK 3
REMARK 3 FIT IN THE HIGHEST RESOLUTION BIN.
REMARK 3 TOTAL NUMBER OF BINS USED : NULL
REMARK 3 BIN RESOLUTION RANGE HIGH (A) : NULL
REMARK 3 BIN RESOLUTION RANGE LOW (A) : NULL
REMARK 3 BIN COMPLETENESS (WORKING+TEST) (%) : NULL
REMARK 3 REFLECTIONS IN BIN (WORKING SET) : NULL
REMARK 3 BIN R VALUE (WORKING SET) : NULL
REMARK 3 BIN FREE R VALUE : NULL
REMARK 3 BIN FREE R VALUE TEST SET SIZE (%) : NULL
REMARK 3 BIN FREE R VALUE TEST SET COUNT : NULL
REMARK 3 ESTIMATED ERROR OF BIN FREE R VALUE : NULL
REMARK 3
REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK 3 PROTEIN ATOMS : 4954
REMARK 3 NUCLEIC ACID ATOMS : 0
REMARK 3 HETEROGEN ATOMS : 0
REMARK 3 SOLVENT ATOMS : 388
REMARK 3
REMARK 3 B VALUES.
REMARK 3 FROM WILSON PLOT (A**2) : NULL
REMARK 3 MEAN B VALUE (OVERALL, A**2) : 25.77
REMARK 3 OVERALL ANISOTROPIC B VALUE.
REMARK 3 B11 (A**2) : 7.73800
REMARK 3 B22 (A**2) : 2.47100
REMARK 3 B33 (A**2) : -10.20900
REMARK 3 B12 (A**2) : 0.00000
REMARK 3 B13 (A**2) : 0.00000
REMARK 3 B23 (A**2) : 0.00000
REMARK 3
REMARK 3 ESTIMATED COORDINATE ERROR.
REMARK 3 ESD FROM LUZZATI PLOT (A) : NULL
REMARK 3 ESD FROM SIGMAA (A) : NULL
REMARK 3 LOW RESOLUTION CUTOFF (A) : NULL
REMARK 3
REMARK 3 CROSS-VALIDATED ESTIMATED COORDINATE ERROR.
REMARK 3 ESD FROM C-V LUZZATI PLOT (A) : NULL
REMARK 3 ESD FROM C-V SIGMAA (A) : NULL
REMARK 3
REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES.
REMARK 3 BOND LENGTHS (A) : 0.005
REMARK 3 BOND ANGLES (DEGREES) : 1.260
REMARK 3 DIHEDRAL ANGLES (DEGREES) : NULL
REMARK 3 IMPROPER ANGLES (DEGREES) : NULL
REMARK 3
REMARK 3 ISOTROPIC THERMAL MODEL : NULL
REMARK 3
REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. RMS SIGMA
REMARK 3 MAIN-CHAIN BOND (A**2) : 1.716 ; 2.000
REMARK 3 MAIN-CHAIN ANGLE (A**2) : 2.487 ; 3.000
REMARK 3 SIDE-CHAIN BOND (A**2) : 2.849 ; 2.500
REMARK 3 SIDE-CHAIN ANGLE (A**2) : 4.302 ; 4.000
REMARK 3
REMARK 3 BULK SOLVENT MODELING.
REMARK 3 METHOD USED : NULL
REMARK 3 KSOL : NULL
REMARK 3 BSOL : 48.84
REMARK 3
REMARK 3 NCS MODEL : NULL
REMARK 3
REMARK 3 NCS RESTRAINTS. RMS SIGMA/WEIGHT
REMARK 3 GROUP 1 POSITIONAL (A) : NULL ; NULL
REMARK 3 GROUP 1 B-FACTOR (A**2) : NULL ; NULL
REMARK 3
REMARK 3 PARAMETER FILE 1 : PROTEIN_REP.PARAM
REMARK 3 PARAMETER FILE 2 : NDP.PAR
REMARK 3 PARAMETER FILE 3 : WATER_REP.PARAM
REMARK 3 PARAMETER FILE 4 : NULL
REMARK 3 TOPOLOGY FILE 1 : NULL
REMARK 3 TOPOLOGY FILE 2 : NULL
REMARK 3 TOPOLOGY FILE 3 : NULL
REMARK 3 TOPOLOGY FILE 4 : NULL
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2QYS COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 31-AUG-07.
REMARK 100 THE DEPOSITION ID IS D_1000044214.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION
REMARK 200 DATE OF DATA COLLECTION : 18-OCT-06
REMARK 200 TEMPERATURE (KELVIN) : 100
REMARK 200 PH : 5.5
REMARK 200 NUMBER OF CRYSTALS USED : 1
REMARK 200
REMARK 200 SYNCHROTRON (Y/N) : Y
REMARK 200 RADIATION SOURCE : ALS
REMARK 200 BEAMLINE : 8.2.2
REMARK 200 X-RAY GENERATOR MODEL : NULL
REMARK 200 MONOCHROMATIC OR LAUE (M/L) : NULL
REMARK 200 WAVELENGTH OR RANGE (A) : 1.0
REMARK 200 MONOCHROMATOR : DOUBLE CRYSTAL SI(111)
REMARK 200 OPTICS : NULL
REMARK 200
REMARK 200 DETECTOR TYPE : CCD
REMARK 200 DETECTOR MANUFACTURER : ADSC QUANTUM 315
REMARK 200 INTENSITY-INTEGRATION SOFTWARE : DENZO
REMARK 200 DATA SCALING SOFTWARE : SCALEPACK
REMARK 200
REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 59871
REMARK 200 RESOLUTION RANGE HIGH (A) : 1.800
REMARK 200 RESOLUTION RANGE LOW (A) : 50.000
REMARK 200 REJECTION CRITERIA (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200 COMPLETENESS FOR RANGE (%) : 95.6
REMARK 200 DATA REDUNDANCY : 7.400
REMARK 200 R MERGE (I) : 0.08600
REMARK 200 R SYM (I) : NULL
REMARK 200 <I/SIGMA(I)> FOR THE DATA SET : 9.6000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.80
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 1.86
REMARK 200 COMPLETENESS FOR SHELL (%) : 73.2
REMARK 200 DATA REDUNDANCY IN SHELL : 5.10
REMARK 200 R MERGE FOR SHELL (I) : 0.47700
REMARK 200 R SYM FOR SHELL (I) : NULL
REMARK 200 <I/SIGMA(I)> FOR SHELL : 2.500
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: CNS
REMARK 200 STARTING MODEL: PDB ENTRY 2QX7
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS (%): 47.89
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.36
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M SODIUM SUCCINATE, 21% PEG 3350,
REMARK 280 0.3 M KCL, 2 MM DITHIOTHREITOL, PH 5.5, VAPOR DIFFUSION, HANGING
REMARK 280 DROP, TEMPERATURE 277K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21
REMARK 290
REMARK 290 SYMOP SYMMETRY
REMARK 290 NNNMMM OPERATOR
REMARK 290 1555 X,Y,Z
REMARK 290 2555 -X+1/2,-Y,Z+1/2
REMARK 290 3555 -X,Y+1/2,-Z+1/2
REMARK 290 4555 X+1/2,-Y+1/2,-Z
REMARK 290
REMARK 290 WHERE NNN -> OPERATOR NUMBER
REMARK 290 MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000
REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000
REMARK 290 SMTRY1 2 -1.000000 0.000000 0.000000 39.70600
REMARK 290 SMTRY2 2 0.000000 -1.000000 0.000000 0.00000
REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 49.09950
REMARK 290 SMTRY1 3 -1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 3 0.000000 1.000000 0.000000 43.16000
REMARK 290 SMTRY3 3 0.000000 0.000000 -1.000000 49.09950
REMARK 290 SMTRY1 4 1.000000 0.000000 0.000000 39.70600
REMARK 290 SMTRY2 4 0.000000 -1.000000 0.000000 43.16000
REMARK 290 SMTRY3 4 0.000000 0.000000 -1.000000 0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 300 REMARK: BIOLOGICAL UNIT IS A MONOMER (HALF OF THE ASYMMETRIC UNIT)
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 SER A -3
REMARK 465 GLY A -2
REMARK 465 HIS A -1
REMARK 465 GLY A 0
REMARK 465 MET A 1
REMARK 465 GLU A 2
REMARK 465 GLU A 3
REMARK 465 ASN A 4
REMARK 465 SER B -3
REMARK 465 GLY B -2
REMARK 465 HIS B -1
REMARK 465 GLY B 0
REMARK 465 MET B 1
REMARK 465 GLU B 2
REMARK 465 GLU B 3
REMARK 465 ASN B 4
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 LYS A 44 54.61 -109.91
REMARK 500 ASP A 111 -88.58 -103.16
REMARK 500 ASN A 152 -147.91 63.13
REMARK 500 ASP A 305 70.58 -152.73
REMARK 500 LYS B 44 58.47 -92.75
REMARK 500 ASP B 111 -89.18 -104.10
REMARK 500 ASN B 152 -147.71 62.93
REMARK 500 ASP B 305 69.52 -156.47
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 2QW8 RELATED DB: PDB
REMARK 900 EUGENOL SYNTHASE, NADP+ MONOCLINIC FORM
REMARK 900 RELATED ID: 2QX7 RELATED DB: PDB
REMARK 900 EUGENOL SYNTHASE, NADP+ ORTHORHOMBIC FORM
REMARK 900 RELATED ID: 2QZ2 RELATED DB: PDB
REMARK 900 EUGENOL SYNTHASE, NADP+, EMDF TERNARY COMPLEX
REMARK 900 RELATED ID: 2R2G RELATED DB: PDB
REMARK 900 EUGENOL SYNTHASE, EMDF
DBREF 2QYS A 1 314 UNP Q15GI4 Q15GI4_OCIBA 1 314
DBREF 2QYS B 1 314 UNP Q15GI4 Q15GI4_OCIBA 1 314
SEQADV 2QYS SER A -3 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS GLY A -2 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS HIS A -1 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS GLY A 0 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS SER B -3 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS GLY B -2 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS HIS B -1 UNP Q15GI4 EXPRESSION TAG
SEQADV 2QYS GLY B 0 UNP Q15GI4 EXPRESSION TAG
SEQRES 1 A 318 SER GLY HIS GLY MET GLU GLU ASN GLY MET LYS SER LYS
SEQRES 2 A 318 ILE LEU ILE PHE GLY GLY THR GLY TYR ILE GLY ASN HIS
SEQRES 3 A 318 MET VAL LYS GLY SER LEU LYS LEU GLY HIS PRO THR TYR
SEQRES 4 A 318 VAL PHE THR ARG PRO ASN SER SER LYS THR THR LEU LEU
SEQRES 5 A 318 ASP GLU PHE GLN SER LEU GLY ALA ILE ILE VAL LYS GLY
SEQRES 6 A 318 GLU LEU ASP GLU HIS GLU LYS LEU VAL GLU LEU MET LYS
SEQRES 7 A 318 LYS VAL ASP VAL VAL ILE SER ALA LEU ALA PHE PRO GLN
SEQRES 8 A 318 ILE LEU ASP GLN PHE LYS ILE LEU GLU ALA ILE LYS VAL
SEQRES 9 A 318 ALA GLY ASN ILE LYS ARG PHE LEU PRO SER ASP PHE GLY
SEQRES 10 A 318 VAL GLU GLU ASP ARG ILE ASN ALA LEU PRO PRO PHE GLU
SEQRES 11 A 318 ALA LEU ILE GLU ARG LYS ARG MET ILE ARG ARG ALA ILE
SEQRES 12 A 318 GLU GLU ALA ASN ILE PRO TYR THR TYR VAL SER ALA ASN
SEQRES 13 A 318 CYS PHE ALA SER TYR PHE ILE ASN TYR LEU LEU ARG PRO
SEQRES 14 A 318 TYR ASP PRO LYS ASP GLU ILE THR VAL TYR GLY THR GLY
SEQRES 15 A 318 GLU ALA LYS PHE ALA MET ASN TYR GLU GLN ASP ILE GLY
SEQRES 16 A 318 LEU TYR THR ILE LYS VAL ALA THR ASP PRO ARG ALA LEU
SEQRES 17 A 318 ASN ARG VAL VAL ILE TYR ARG PRO SER THR ASN ILE ILE
SEQRES 18 A 318 THR GLN LEU GLU LEU ILE SER ARG TRP GLU LYS LYS ILE
SEQRES 19 A 318 GLY LYS LYS PHE LYS LYS ILE HIS VAL PRO GLU GLU GLU
SEQRES 20 A 318 ILE VAL ALA LEU THR LYS GLU LEU PRO GLU PRO GLU ASN
SEQRES 21 A 318 ILE PRO ILE ALA ILE LEU HIS CYS LEU PHE ILE ASP GLY
SEQRES 22 A 318 ALA THR MET SER TYR ASP PHE LYS GLU ASN ASP VAL GLU
SEQRES 23 A 318 ALA SER THR LEU TYR PRO GLU LEU LYS PHE THR THR ILE
SEQRES 24 A 318 ASP GLU LEU LEU ASP ILE PHE VAL HIS ASP PRO PRO PRO
SEQRES 25 A 318 PRO ALA SER ALA ALA PHE
SEQRES 1 B 318 SER GLY HIS GLY MET GLU GLU ASN GLY MET LYS SER LYS
SEQRES 2 B 318 ILE LEU ILE PHE GLY GLY THR GLY TYR ILE GLY ASN HIS
SEQRES 3 B 318 MET VAL LYS GLY SER LEU LYS LEU GLY HIS PRO THR TYR
SEQRES 4 B 318 VAL PHE THR ARG PRO ASN SER SER LYS THR THR LEU LEU
SEQRES 5 B 318 ASP GLU PHE GLN SER LEU GLY ALA ILE ILE VAL LYS GLY
SEQRES 6 B 318 GLU LEU ASP GLU HIS GLU LYS LEU VAL GLU LEU MET LYS
SEQRES 7 B 318 LYS VAL ASP VAL VAL ILE SER ALA LEU ALA PHE PRO GLN
SEQRES 8 B 318 ILE LEU ASP GLN PHE LYS ILE LEU GLU ALA ILE LYS VAL
SEQRES 9 B 318 ALA GLY ASN ILE LYS ARG PHE LEU PRO SER ASP PHE GLY
SEQRES 10 B 318 VAL GLU GLU ASP ARG ILE ASN ALA LEU PRO PRO PHE GLU
SEQRES 11 B 318 ALA LEU ILE GLU ARG LYS ARG MET ILE ARG ARG ALA ILE
SEQRES 12 B 318 GLU GLU ALA ASN ILE PRO TYR THR TYR VAL SER ALA ASN
SEQRES 13 B 318 CYS PHE ALA SER TYR PHE ILE ASN TYR LEU LEU ARG PRO
SEQRES 14 B 318 TYR ASP PRO LYS ASP GLU ILE THR VAL TYR GLY THR GLY
SEQRES 15 B 318 GLU ALA LYS PHE ALA MET ASN TYR GLU GLN ASP ILE GLY
SEQRES 16 B 318 LEU TYR THR ILE LYS VAL ALA THR ASP PRO ARG ALA LEU
SEQRES 17 B 318 ASN ARG VAL VAL ILE TYR ARG PRO SER THR ASN ILE ILE
SEQRES 18 B 318 THR GLN LEU GLU LEU ILE SER ARG TRP GLU LYS LYS ILE
SEQRES 19 B 318 GLY LYS LYS PHE LYS LYS ILE HIS VAL PRO GLU GLU GLU
SEQRES 20 B 318 ILE VAL ALA LEU THR LYS GLU LEU PRO GLU PRO GLU ASN
SEQRES 21 B 318 ILE PRO ILE ALA ILE LEU HIS CYS LEU PHE ILE ASP GLY
SEQRES 22 B 318 ALA THR MET SER TYR ASP PHE LYS GLU ASN ASP VAL GLU
SEQRES 23 B 318 ALA SER THR LEU TYR PRO GLU LEU LYS PHE THR THR ILE
SEQRES 24 B 318 ASP GLU LEU LEU ASP ILE PHE VAL HIS ASP PRO PRO PRO
SEQRES 25 B 318 PRO ALA SER ALA ALA PHE
FORMUL 3 HOH *388(H2 O)
HELIX 1 1 ILE A 19 LEU A 30 1 12
HELIX 2 2 LYS A 44 LEU A 54 1 11
HELIX 3 3 GLU A 65 LYS A 74 1 10
HELIX 4 4 ALA A 84 LEU A 89 5 6
HELIX 5 5 ASP A 90 GLY A 102 1 13
HELIX 6 6 LEU A 122 ALA A 142 1 21
HELIX 7 7 ALA A 155 ARG A 164 1 10
HELIX 8 8 TYR A 186 THR A 199 1 14
HELIX 9 9 ASP A 200 LEU A 204 5 5
HELIX 10 10 PRO A 212 THR A 214 5 3
HELIX 11 11 GLN A 219 GLY A 231 1 13
HELIX 12 12 PRO A 240 LEU A 251 1 12
HELIX 13 13 GLU A 255 ILE A 267 1 13
HELIX 14 14 SER A 284 LEU A 286 5 3
HELIX 15 15 THR A 294 ASP A 305 1 12
HELIX 16 16 ILE B 19 LEU B 30 1 12
HELIX 17 17 LYS B 44 LEU B 54 1 11
HELIX 18 18 GLU B 65 LYS B 74 1 10
HELIX 19 19 ALA B 84 LEU B 89 5 6
HELIX 20 20 ASP B 90 GLY B 102 1 13
HELIX 21 21 LEU B 122 ALA B 142 1 21
HELIX 22 22 ALA B 155 ARG B 164 1 10
HELIX 23 23 TYR B 186 THR B 199 1 14
HELIX 24 24 ASP B 200 LEU B 204 5 5
HELIX 25 25 PRO B 212 THR B 214 5 3
HELIX 26 26 GLN B 219 GLY B 231 1 13
HELIX 27 27 PRO B 240 LEU B 251 1 12
HELIX 28 28 GLU B 255 ILE B 267 1 13
HELIX 29 29 SER B 284 LEU B 286 5 3
HELIX 30 30 THR B 294 ASP B 305 1 12
SHEET 1 A 5 ILE A 57 LYS A 60 0
SHEET 2 A 5 THR A 34 THR A 38 1 N THR A 34 O ILE A 57
SHEET 3 A 5 ILE A 10 PHE A 13 1 N ILE A 10 O TYR A 35
SHEET 4 A 5 VAL A 78 SER A 81 1 O VAL A 78 N LEU A 11
SHEET 5 A 5 ARG A 106 LEU A 108 1 O LEU A 108 N SER A 81
SHEET 1 B 3 THR A 147 SER A 150 0
SHEET 2 B 3 ARG A 206 ILE A 209 1 O VAL A 208 N TYR A 148
SHEET 3 B 3 VAL A 281 GLU A 282 1 O VAL A 281 N ILE A 209
SHEET 1 C 3 CYS A 153 PHE A 154 0
SHEET 2 C 3 LYS A 181 ASN A 185 1 O ASN A 185 N CYS A 153
SHEET 3 C 3 ILE A 216 THR A 218 -1 O ILE A 217 N PHE A 182
SHEET 1 D 2 GLU A 171 TYR A 175 0
SHEET 2 D 2 LYS A 235 VAL A 239 1 O ILE A 237 N ILE A 172
SHEET 1 E 5 ILE B 57 LYS B 60 0
SHEET 2 E 5 THR B 34 THR B 38 1 N THR B 34 O ILE B 57
SHEET 3 E 5 ILE B 10 PHE B 13 1 N ILE B 10 O TYR B 35
SHEET 4 E 5 VAL B 78 SER B 81 1 O VAL B 78 N LEU B 11
SHEET 5 E 5 ARG B 106 LEU B 108 1 O LEU B 108 N SER B 81
SHEET 1 F 3 THR B 147 SER B 150 0
SHEET 2 F 3 ARG B 206 ILE B 209 1 O VAL B 208 N TYR B 148
SHEET 3 F 3 VAL B 281 GLU B 282 1 O VAL B 281 N ILE B 209
SHEET 1 G 3 CYS B 153 PHE B 154 0
SHEET 2 G 3 LYS B 181 ASN B 185 1 O ASN B 185 N CYS B 153
SHEET 3 G 3 ILE B 216 THR B 218 -1 O ILE B 217 N PHE B 182
SHEET 1 H 2 GLU B 171 TYR B 175 0
SHEET 2 H 2 LYS B 235 VAL B 239 1 O ILE B 237 N ILE B 172
CISPEP 1 GLU A 253 PRO A 254 0 0.06
CISPEP 2 GLU B 253 PRO B 254 0 2.06
CRYST1 79.412 86.320 98.199 90.00 90.00 90.00 P 21 21 21 8
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 0.012593 0.000000 0.000000 0.00000
SCALE2 0.000000 0.011585 0.000000 0.00000
SCALE3 0.000000 0.000000 0.010183 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
