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Database: PDB
Entry: 2STT
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HEADER    DNA BINDING PROTEIN/DNA                 05-AUG-96   2STT              
TITLE     SOLUTION NMR STRUCTURE OF THE HUMAN ETS1/DNA COMPLEX, 25              
TITLE    2 STRUCTURES                                                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DNA (5'-                                                   
COMPND   3 D(*TP*CP*GP*AP*GP*CP*CP*GP*GP*AP*AP*GP*TP*TP*CP*GP*A)-3');           
COMPND   4 CHAIN: B;                                                            
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: DNA (5'-                                                   
COMPND   8 D(*TP*CP*GP*AP*AP*CP*TP*TP*CP*CP*GP*GP*CP*TP*CP*GP*A)-3');           
COMPND   9 CHAIN: C;                                                            
COMPND  10 ENGINEERED: YES;                                                     
COMPND  11 MOL_ID: 3;                                                           
COMPND  12 MOLECULE: ETS1;                                                      
COMPND  13 CHAIN: A                                                             
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 MOL_ID: 2;                                                           
SOURCE   4 SYNTHETIC: YES;                                                      
SOURCE   5 MOL_ID: 3;                                                           
SOURCE   6 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   7 ORGANISM_COMMON: HUMAN;                                              
SOURCE   8 ORGANISM_TAXID: 9606                                                 
KEYWDS    DEOXYRIBONUCLEIC ACID, COMPLEX (DNA-BINDING PROTEIN/DNA),             
KEYWDS   2 DNA BINDING PROTEIN/DNA COMPLEX                                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    25                                                                    
AUTHOR    G.M.CLORE,M.H.WERNER,A.M.GRONENBORN                                   
REVDAT   2   24-FEB-09 2STT    1       VERSN                                    
REVDAT   1   12-MAR-97 2STT    0                                                
SPRSDE     12-MAR-97 2STT      1STT                                             
JRNL        AUTH   M.H.WERNER,G.M.CLORE,C.L.FISHER,R.J.FISHER,L.TRINH,          
JRNL        AUTH 2 J.SHILOACH,A.M.GRONENBORN                                    
JRNL        TITL   CORRECTION OF THE NMR STRUCTURE OF THE ETS1/DNA              
JRNL        TITL 2 COMPLEX.                                                     
JRNL        REF    J.BIOMOL.NMR                  V.  10   317 1997              
JRNL        REFN                   ISSN 0925-2738                               
JRNL        PMID   9460239                                                      
JRNL        DOI    10.1023/A:1018399711996                                      
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : XPLOR                                                
REMARK   3   AUTHORS     : JONES,ZOU,COWAN,KJELDGAARD                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS:                                           
REMARK   3  THE RESTRAINED REGULARIZED MEAN STRUCTURE IS PRESENTED IN           
REMARK   3  ENTRY 2STW AND 25 STRUCTURES ARE PRESENTED IN ENTRY 2STT.           
REMARK   3  IN 2STW THE LAST COLUMN REPRESENTS THE RMS OF THE 25                
REMARK   3  INDIVIDUAL SIMULATED ANNEALING STRUCTURES ABOUT THE MEAN            
REMARK   3  COORDINATE POSITIONS.  THE LAST COLUMN IN THE INDIVIDUAL SA         
REMARK   3  STRUCTURES HAS NO MEANING.  BEST FITTING TO GENERATE THE            
REMARK   3  AVERAGE STRUCTURE IS WITH RESPECT TO RESIDUES 24 - 105 OF           
REMARK   3  THE PROTEIN AND BASE PAIRS 1 - 17 OF THE DNA (RESIDUES 10 -         
REMARK   3  24 ARE DISORDERED IN SOLUTION).  RESIDUE 10 CORRESPONDS TO          
REMARK   3  RESIDUE 320 OF THE NATURAL SEQUENCE.  NOTE THE OCCUPANCY            
REMARK   3  FIELD HAS NO MEANING.                                               
REMARK   4                                                                      
REMARK   4 2STT COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 305.00                             
REMARK 210  PH                             : 6.80                               
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : AMX500, AMX600                     
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XPLOR                              
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 25                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500  1  DT C  25   C5     DT C  25   C7      0.038                       
REMARK 500  1  DT C  31   C5     DT C  31   C7      0.038                       
REMARK 500  3  DT B  13   C5     DT B  13   C7      0.036                       
REMARK 500  3  DT C  25   C5     DT C  25   C7      0.042                       
REMARK 500  3  DT C  31   C5     DT C  31   C7      0.040                       
REMARK 500  4  DT C  31   C5     DT C  31   C7      0.037                       
REMARK 500  5  DT C  24   C5     DT C  24   C7      0.037                       
REMARK 500  5  DT C  31   C5     DT C  31   C7      0.042                       
REMARK 500  6  DT B  14   C5     DT B  14   C7      0.037                       
REMARK 500  7  DT B  14   C5     DT B  14   C7      0.039                       
REMARK 500  7  DT C  25   C5     DT C  25   C7      0.038                       
REMARK 500  7  DT C  31   C5     DT C  31   C7      0.037                       
REMARK 500  8  DT C  24   C5     DT C  24   C7      0.036                       
REMARK 500  8  DT C  31   C5     DT C  31   C7      0.040                       
REMARK 500  9  DT B  14   C5     DT B  14   C7      0.039                       
REMARK 500 10  DT C  25   C5     DT C  25   C7      0.039                       
REMARK 500 10  DT C  31   C5     DT C  31   C7      0.038                       
REMARK 500 11  DT B  14   C5     DT B  14   C7      0.036                       
REMARK 500 11  DT C  31   C5     DT C  31   C7      0.038                       
REMARK 500 12  DT B  14   C5     DT B  14   C7      0.038                       
REMARK 500 12  DT C  31   C5     DT C  31   C7      0.043                       
REMARK 500 13  DT B  14   C5     DT B  14   C7      0.039                       
REMARK 500 14  DT B  13   C5     DT B  13   C7      0.037                       
REMARK 500 14  DT C  25   C5     DT C  25   C7      0.038                       
REMARK 500 14  DT C  31   C5     DT C  31   C7      0.038                       
REMARK 500 16  DT B  13   C5     DT B  13   C7      0.042                       
REMARK 500 16  DT C  24   C5     DT C  24   C7      0.036                       
REMARK 500 16  DT C  25   C5     DT C  25   C7      0.037                       
REMARK 500 16  DT C  31   C5     DT C  31   C7      0.039                       
REMARK 500 17  DT B  14   C5     DT B  14   C7      0.037                       
REMARK 500 17  DT C  25   C5     DT C  25   C7      0.038                       
REMARK 500 18  DT B  13   C5     DT B  13   C7      0.040                       
REMARK 500 18  DT B  14   C5     DT B  14   C7      0.037                       
REMARK 500 18  DT C  18   C5     DT C  18   C7      0.040                       
REMARK 500 18  DT C  24   C5     DT C  24   C7      0.037                       
REMARK 500 18  DT C  31   C5     DT C  31   C7      0.041                       
REMARK 500 20  DT C  31   C5     DT C  31   C7      0.038                       
REMARK 500 21  DT C  24   C5     DT C  24   C7      0.039                       
REMARK 500 21  DT C  25   C5     DT C  25   C7      0.036                       
REMARK 500 21  DT C  31   C5     DT C  31   C7      0.039                       
REMARK 500 22  DT C  31   C5     DT C  31   C7      0.041                       
REMARK 500 23  DT B  13   C5     DT B  13   C7      0.036                       
REMARK 500 23  DT B  14   C5     DT B  14   C7      0.037                       
REMARK 500 23  DT C  25   C5     DT C  25   C7      0.038                       
REMARK 500 23  DT C  31   C5     DT C  31   C7      0.036                       
REMARK 500 25  DT B  13   C5     DT B  13   C7      0.036                       
REMARK 500 25  DT C  25   C5     DT C  25   C7      0.042                       
REMARK 500 25  DT C  31   C5     DT C  31   C7      0.040                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1  DT B   1   O4' -  C1' -  N1  ANGL. DEV. =   3.4 DEGREES          
REMARK 500  1  DT B   1   C6  -  C5  -  C7  ANGL. DEV. =  -3.7 DEGREES          
REMARK 500  1  DC B   2   O4' -  C1' -  N1  ANGL. DEV. =   2.8 DEGREES          
REMARK 500  1  DG B   3   O4' -  C1' -  N9  ANGL. DEV. =   3.0 DEGREES          
REMARK 500  1  DA B   4   O4' -  C1' -  N9  ANGL. DEV. =   2.6 DEGREES          
REMARK 500  1  DG B   5   O4' -  C1' -  N9  ANGL. DEV. =   3.4 DEGREES          
REMARK 500  1  DC B   6   O4' -  C1' -  N1  ANGL. DEV. =   3.1 DEGREES          
REMARK 500  1  DC B   7   O4' -  C1' -  N1  ANGL. DEV. =   3.7 DEGREES          
REMARK 500  1  DG B   8   O4' -  C1' -  N9  ANGL. DEV. =   2.6 DEGREES          
REMARK 500  1  DG B   9   O4' -  C1' -  N9  ANGL. DEV. =   3.6 DEGREES          
REMARK 500  1  DA B  10   O4' -  C1' -  N9  ANGL. DEV. =   3.3 DEGREES          
REMARK 500  1  DA B  11   O4' -  C1' -  N9  ANGL. DEV. =   3.1 DEGREES          
REMARK 500  1  DG B  12   O4' -  C1' -  N9  ANGL. DEV. =   3.0 DEGREES          
REMARK 500  1  DT B  13   O4' -  C1' -  N1  ANGL. DEV. =   3.0 DEGREES          
REMARK 500  1  DT B  14   O4' -  C1' -  N1  ANGL. DEV. =   3.4 DEGREES          
REMARK 500  1  DC B  15   O4' -  C1' -  N1  ANGL. DEV. =   1.8 DEGREES          
REMARK 500  1  DG B  16   O4' -  C1' -  N9  ANGL. DEV. =   3.0 DEGREES          
REMARK 500  1  DA B  17   O4' -  C1' -  N9  ANGL. DEV. =   3.5 DEGREES          
REMARK 500  1  DT C  18   O4' -  C1' -  N1  ANGL. DEV. =   2.6 DEGREES          
REMARK 500  1  DC C  19   O4' -  C1' -  N1  ANGL. DEV. =   2.3 DEGREES          
REMARK 500  1  DG C  20   O4' -  C1' -  N9  ANGL. DEV. =   2.0 DEGREES          
REMARK 500  1  DA C  21   O4' -  C1' -  N9  ANGL. DEV. =   3.3 DEGREES          
REMARK 500  1  DA C  22   O4' -  C1' -  N9  ANGL. DEV. =   3.0 DEGREES          
REMARK 500  1  DC C  23   O4' -  C1' -  N1  ANGL. DEV. =   3.6 DEGREES          
REMARK 500  1  DT C  24   O4' -  C1' -  N1  ANGL. DEV. =   2.3 DEGREES          
REMARK 500  1  DT C  25   O4' -  C1' -  N1  ANGL. DEV. =   4.1 DEGREES          
REMARK 500  1  DC C  26   O4' -  C1' -  N1  ANGL. DEV. =   3.3 DEGREES          
REMARK 500  1  DC C  27   O4' -  C1' -  N1  ANGL. DEV. =   2.5 DEGREES          
REMARK 500  1  DG C  28   O4' -  C1' -  N9  ANGL. DEV. =   2.7 DEGREES          
REMARK 500  1  DG C  29   O4' -  C1' -  N9  ANGL. DEV. =   3.7 DEGREES          
REMARK 500  1  DC C  30   O4' -  C1' -  N1  ANGL. DEV. =   2.7 DEGREES          
REMARK 500  1  DT C  31   O4' -  C1' -  N1  ANGL. DEV. =   2.9 DEGREES          
REMARK 500  1  DC C  32   O4' -  C1' -  N1  ANGL. DEV. =   2.1 DEGREES          
REMARK 500  1  DG C  33   O4' -  C1' -  N9  ANGL. DEV. =   2.2 DEGREES          
REMARK 500  1  DA C  34   O4' -  C1' -  N9  ANGL. DEV. =   2.5 DEGREES          
REMARK 500  2  DT B   1   O4' -  C1' -  N1  ANGL. DEV. =   2.8 DEGREES          
REMARK 500  2  DC B   2   O4' -  C1' -  N1  ANGL. DEV. =   2.9 DEGREES          
REMARK 500  2  DG B   3   O4' -  C1' -  N9  ANGL. DEV. =   2.9 DEGREES          
REMARK 500  2  DA B   4   O4' -  C1' -  N9  ANGL. DEV. =   4.1 DEGREES          
REMARK 500  2  DG B   5   O4' -  C1' -  N9  ANGL. DEV. =   2.4 DEGREES          
REMARK 500  2  DC B   6   O4' -  C1' -  N1  ANGL. DEV. =   3.2 DEGREES          
REMARK 500  2  DC B   7   O4' -  C1' -  N1  ANGL. DEV. =   3.3 DEGREES          
REMARK 500  2  DG B   8   O4' -  C1' -  N9  ANGL. DEV. =   3.1 DEGREES          
REMARK 500  2  DG B   9   O4' -  C1' -  N9  ANGL. DEV. =   3.7 DEGREES          
REMARK 500  2  DA B  10   O4' -  C1' -  N9  ANGL. DEV. =   3.6 DEGREES          
REMARK 500  2  DA B  11   O4' -  C1' -  N9  ANGL. DEV. =   2.8 DEGREES          
REMARK 500  2  DG B  12   O4' -  C1' -  N9  ANGL. DEV. =   3.0 DEGREES          
REMARK 500  2  DT B  13   O4' -  C1' -  N1  ANGL. DEV. =   3.6 DEGREES          
REMARK 500  2  DT B  14   O4' -  C1' -  N1  ANGL. DEV. =   2.8 DEGREES          
REMARK 500  2  DC B  15   O4' -  C1' -  N1  ANGL. DEV. =   1.8 DEGREES          
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     849 ANGLE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 PRO A  12        4.56    -68.08                                   
REMARK 500  1 ALA A  13      -91.79    -71.33                                   
REMARK 500  1 ASP A  49     -112.16   -115.92                                   
REMARK 500  1 LYS A  89      -93.43   -126.05                                   
REMARK 500  1 ASN A  90       92.98   -160.51                                   
REMARK 500  1 ILE A  91      -91.62   -144.92                                   
REMARK 500  2 THR A  20     -112.11   -126.31                                   
REMARK 500  2 SER A  22       40.58   -106.74                                   
REMARK 500  2 ASP A  37      -84.64   -106.53                                   
REMARK 500  2 ASP A  49     -108.49   -107.60                                   
REMARK 500  2 PRO A  72      -86.61    -57.09                                   
REMARK 500  2 TYR A  86        7.03    -69.25                                   
REMARK 500  2 TYR A  87       98.06    -62.11                                   
REMARK 500  2 ASP A  88      -77.62    -87.24                                   
REMARK 500  2 LYS A  89      -66.52   -126.91                                   
REMARK 500  2 ASN A  90       84.92   -154.42                                   
REMARK 500  2 ILE A  91     -100.77   -139.39                                   
REMARK 500  3 PRO A  12       29.47    -60.91                                   
REMARK 500  3 ALA A  13     -169.64   -103.77                                   
REMARK 500  3 THR A  20     -158.12    -94.64                                   
REMARK 500  3 THR A  36       52.45   -108.30                                   
REMARK 500  3 ASP A  37      -80.16   -128.50                                   
REMARK 500  3 LYS A  38     -169.13    -76.93                                   
REMARK 500  3 SER A  39     -131.60    -74.12                                   
REMARK 500  3 CYS A  40      -44.79   -174.62                                   
REMARK 500  3 ASP A  49      -45.19   -138.10                                   
REMARK 500  3 TRP A  51       50.25   -159.47                                   
REMARK 500  3 PRO A  58       57.37    -67.53                                   
REMARK 500  3 ILE A  91      -49.19   -136.03                                   
REMARK 500  3 ALA A  96        6.63    -68.61                                   
REMARK 500  4 THR A  36       70.44   -113.44                                   
REMARK 500  4 ASP A  37     -135.46   -115.31                                   
REMARK 500  4 LYS A  38     -143.12    -59.55                                   
REMARK 500  4 CYS A  40      -19.05     70.43                                   
REMARK 500  4 ASP A  49     -153.48   -122.43                                   
REMARK 500  4 TRP A  51       69.99   -150.66                                   
REMARK 500  4 PRO A  72     -159.05    -52.84                                   
REMARK 500  4 ILE A  91      -46.78   -136.19                                   
REMARK 500  4 ALA A  96       26.83    -75.96                                   
REMARK 500  4 LYS A  98     -133.79   -113.32                                   
REMARK 500  5 ALA A  17      -89.08    -79.83                                   
REMARK 500  5 THR A  47     -158.84    -58.11                                   
REMARK 500  5 TRP A  51       35.44   -155.34                                   
REMARK 500  5 PRO A  58       38.54    -72.17                                   
REMARK 500  5 PRO A  72      -83.66    -60.40                                   
REMARK 500  5 TYR A  87        3.80    -67.22                                   
REMARK 500  5 ILE A  91      -48.19   -136.63                                   
REMARK 500  5 ALA A  96       33.13    -84.11                                   
REMARK 500  5 LYS A  98     -119.81   -111.62                                   
REMARK 500  5 ARG A  99      -39.52   -131.95                                   
REMARK 500  6 ALA A  17     -111.74    -78.71                                   
REMARK 500  6 ASP A  37      -73.09   -107.61                                   
REMARK 500  6 SER A  39     -132.15    -67.25                                   
REMARK 500  6 CYS A  40      -36.07   -171.42                                   
REMARK 500  6 SER A  42      -49.80   -163.16                                   
REMARK 500  6 ASP A  49     -108.38   -108.01                                   
REMARK 500  6 PRO A  72     -157.04    -53.28                                   
REMARK 500  6 TYR A  87        8.91    -69.75                                   
REMARK 500  6 ILE A  91      -44.90   -138.27                                   
REMARK 500  6 ALA A  96       32.61    -83.37                                   
REMARK 500  6 LYS A  98     -117.87   -110.27                                   
REMARK 500  6 ARG A  99      -31.41   -134.26                                   
REMARK 500  7 ILE A  11       45.45   -142.36                                   
REMARK 500  7 ASP A  37     -135.62   -131.94                                   
REMARK 500  7 LYS A  38     -165.36    -62.47                                   
REMARK 500  7 CYS A  40       -1.34     58.34                                   
REMARK 500  7 ASP A  49     -114.32   -109.62                                   
REMARK 500  7 ILE A  91      -85.46   -132.86                                   
REMARK 500  7 ALA A  96       28.30    -77.43                                   
REMARK 500  7 LYS A  98     -115.44   -106.13                                   
REMARK 500  7 ARG A  99      -39.65   -131.32                                   
REMARK 500  8 ILE A  11       55.62   -140.14                                   
REMARK 500  8 ASP A  37      -63.56   -124.79                                   
REMARK 500  8 LYS A  38     -160.54   -103.51                                   
REMARK 500  8 ASP A  49     -102.78   -117.43                                   
REMARK 500  8 PRO A  58       42.14    -74.52                                   
REMARK 500  8 PRO A  72     -153.15    -61.98                                   
REMARK 500  8 ILE A  91      -89.86   -138.02                                   
REMARK 500  8 LYS A  98     -134.32   -127.34                                   
REMARK 500  8 ARG A  99      -30.41   -136.59                                   
REMARK 500  9 ALA A  17      -96.11    -83.81                                   
REMARK 500  9 PRO A  24     -177.84    -57.86                                   
REMARK 500  9 ASP A  37      -96.33   -101.69                                   
REMARK 500  9 CYS A  40       -4.66     71.24                                   
REMARK 500  9 ASP A  49     -121.64   -110.46                                   
REMARK 500  9 PRO A  58       49.40    -74.38                                   
REMARK 500  9 PRO A  72      -87.32    -59.28                                   
REMARK 500  9 TYR A  86        2.20    -69.03                                   
REMARK 500  9 LYS A  89      -98.58   -113.31                                   
REMARK 500  9 ASN A  90       84.39   -162.00                                   
REMARK 500  9 ILE A  91      -51.35   -128.61                                   
REMARK 500 10 PRO A  12        0.73    -68.81                                   
REMARK 500 10 ALA A  13      -77.84    -60.19                                   
REMARK 500 10 THR A  36       62.12   -104.80                                   
REMARK 500 10 ASP A  37     -120.61   -136.15                                   
REMARK 500 10 CYS A  40       16.60     58.69                                   
REMARK 500 10 ASP A  49      -94.37   -126.44                                   
REMARK 500 10 TRP A  51       57.35   -140.17                                   
REMARK 500 10 PRO A  72     -175.96    -49.87                                   
REMARK 500 10 ILE A  91      -84.79   -138.30                                   
REMARK 500 10 LYS A  98      -41.70   -142.36                                   
REMARK 500 10 TYR A 100       48.35    -73.94                                   
REMARK 500 11 PRO A  12       -4.06    -53.57                                   
REMARK 500 11 ALA A  13      -82.96    -49.28                                   
REMARK 500 11 ASP A  37     -139.66   -119.08                                   
REMARK 500 11 LYS A  38     -177.97    -56.82                                   
REMARK 500 11 CYS A  40       17.36     56.11                                   
REMARK 500 11 ILE A  44     -167.28   -163.79                                   
REMARK 500 11 ASP A  49     -149.69   -113.66                                   
REMARK 500 11 TRP A  51       79.25   -165.62                                   
REMARK 500 11 PRO A  58       64.44    -63.93                                   
REMARK 500 11 PRO A  72     -166.55    -55.23                                   
REMARK 500 11 ALA A  96       47.13    -88.68                                   
REMARK 500 11 LYS A  98      -71.11   -111.83                                   
REMARK 500 11 ARG A  99      -57.39   -170.97                                   
REMARK 500 12 ILE A  11       59.34   -141.51                                   
REMARK 500 12 THR A  20     -139.55   -101.40                                   
REMARK 500 12 ASP A  37     -104.44   -125.73                                   
REMARK 500 12 ASP A  49      -97.52   -112.59                                   
REMARK 500 12 TRP A  51       82.21   -156.62                                   
REMARK 500 12 PRO A  58       33.05    -72.18                                   
REMARK 500 12 PRO A  72      -86.45    -50.57                                   
REMARK 500 12 TYR A  87        7.83    -68.51                                   
REMARK 500 12 ILE A  91      -84.14   -140.12                                   
REMARK 500 13 ALA A  13      -83.41    -52.36                                   
REMARK 500 13 ALA A  15      -74.56    -87.62                                   
REMARK 500 13 ILE A  25     -149.42    -80.78                                   
REMARK 500 13 THR A  36      -79.99    -68.86                                   
REMARK 500 13 ILE A  44     -167.56   -161.60                                   
REMARK 500 13 ASP A  49     -103.75   -115.30                                   
REMARK 500 13 PRO A  58       69.88    -67.22                                   
REMARK 500 13 PRO A  72        2.50    -69.50                                   
REMARK 500 13 ILE A  91      -43.98   -137.32                                   
REMARK 500 14 LEU A  16      -79.72    -89.25                                   
REMARK 500 14 LYS A  38     -160.77   -104.91                                   
REMARK 500 14 ASP A  49     -124.64   -122.92                                   
REMARK 500 14 TRP A  51       76.46   -159.89                                   
REMARK 500 14 PRO A  58       50.67    -65.15                                   
REMARK 500 14 TYR A  87        6.08    -65.71                                   
REMARK 500 14 ILE A  91      -49.07   -135.39                                   
REMARK 500 14 LYS A  98      -64.36   -120.75                                   
REMARK 500 14 ARG A  99      -38.84   -171.90                                   
REMARK 500 15 ALA A  17      -89.70    -79.88                                   
REMARK 500 15 THR A  36       50.29   -108.07                                   
REMARK 500 15 ASP A  37     -115.30   -107.46                                   
REMARK 500 15 LYS A  38     -171.63    -64.60                                   
REMARK 500 15 CYS A  40        1.30     59.24                                   
REMARK 500 15 ASP A  49     -106.52   -109.15                                   
REMARK 500 15 TYR A  87       20.75    -78.93                                   
REMARK 500 15 ILE A  91      -87.29   -142.19                                   
REMARK 500 16 ILE A  11       36.87   -141.44                                   
REMARK 500 16 ALA A  17      -81.32    -81.71                                   
REMARK 500 16 TYR A  19       80.04    -68.44                                   
REMARK 500 16 THR A  36       61.16   -109.42                                   
REMARK 500 16 ASP A  37     -104.31   -136.11                                   
REMARK 500 16 LYS A  38      174.38    -55.82                                   
REMARK 500 16 SER A  42      -55.90   -159.86                                   
REMARK 500 16 ASP A  49      -94.36   -120.87                                   
REMARK 500 16 TRP A  51       49.37   -151.09                                   
REMARK 500 16 PRO A  58       46.24    -68.38                                   
REMARK 500 16 PRO A  72      -81.74    -60.20                                   
REMARK 500 16 TYR A  87        8.40    -68.89                                   
REMARK 500 16 ILE A  91      -42.94   -137.31                                   
REMARK 500 16 ALA A  96       37.27    -76.21                                   
REMARK 500 16 LYS A  98     -111.77   -108.10                                   
REMARK 500 16 ARG A  99      -30.53   -134.93                                   
REMARK 500 17 PRO A  12       67.66    -65.64                                   
REMARK 500 17 ALA A  13     -102.16   -107.95                                   
REMARK 500 17 ASP A  37      -60.74   -135.46                                   
REMARK 500 17 LYS A  38     -158.92    -59.94                                   
REMARK 500 17 SER A  39      -90.04    -69.54                                   
REMARK 500 17 CYS A  40      -65.98    160.52                                   
REMARK 500 17 ILE A  44     -168.99   -165.03                                   
REMARK 500 17 ASP A  49     -108.63   -126.19                                   
REMARK 500 17 PRO A  58       62.23    -62.60                                   
REMARK 500 17 TYR A  87       20.95    -78.11                                   
REMARK 500 17 ILE A  91      -62.54   -137.42                                   
REMARK 500 17 ALA A  96       30.55    -80.52                                   
REMARK 500 17 LYS A  98     -123.62   -114.26                                   
REMARK 500 18 PRO A  12       62.66    -58.35                                   
REMARK 500 18 LEU A  16      -73.75    -74.86                                   
REMARK 500 18 ALA A  17     -126.64    -95.45                                   
REMARK 500 18 THR A  36       48.68    -99.93                                   
REMARK 500 18 ASP A  37     -114.69   -132.21                                   
REMARK 500 18 CYS A  40       16.21     51.49                                   
REMARK 500 18 ASP A  49     -105.46   -116.39                                   
REMARK 500 18 PRO A  72     -148.84    -59.17                                   
REMARK 500 18 ILE A  91      -87.60   -137.89                                   
REMARK 500 18 ARG A  99      -64.86   -171.79                                   
REMARK 500 18 TYR A 100       40.16   -104.38                                   
REMARK 500 19 ALA A  17     -141.58    -82.66                                   
REMARK 500 19 ASP A  37      -80.61   -126.34                                   
REMARK 500 19 LYS A  38     -167.31    -73.96                                   
REMARK 500 19 CYS A  40      -50.48     79.99                                   
REMARK 500 19 ILE A  44     -168.32   -162.78                                   
REMARK 500 19 THR A  47     -170.52    -53.53                                   
REMARK 500 19 TRP A  51       33.71   -163.77                                   
REMARK 500 19 PRO A  58       28.32    -74.85                                   
REMARK 500 19 PRO A  72       -8.97    -58.16                                   
REMARK 500 19 ILE A  91      -89.04   -138.69                                   
REMARK 500 19 ALA A  96       31.53    -84.16                                   
REMARK 500 19 LYS A  98      -81.70   -108.11                                   
REMARK 500 19 ARG A  99      -40.10   -170.51                                   
REMARK 500 20 ALA A  13      -78.45    -49.49                                   
REMARK 500 20 ALA A  17     -149.52    -89.40                                   
REMARK 500 20 ASP A  37      -74.34   -132.57                                   
REMARK 500 20 LYS A  38     -163.52   -118.00                                   
REMARK 500 20 CYS A  40        0.36     57.60                                   
REMARK 500 20 THR A  47     -173.77    -49.53                                   
REMARK 500 20 TRP A  51       37.28   -170.31                                   
REMARK 500 20 PRO A  58       55.75    -66.55                                   
REMARK 500 20 PRO A  72      -79.46    -48.72                                   
REMARK 500 20 LYS A  89      -90.39   -117.14                                   
REMARK 500 20 ILE A  91      -89.38   -136.56                                   
REMARK 500 20 LYS A  98     -161.83   -105.96                                   
REMARK 500 21 THR A  36       49.02    -93.14                                   
REMARK 500 21 CYS A  40      -72.22    -82.00                                   
REMARK 500 21 SER A  42      -26.13     84.65                                   
REMARK 500 21 ILE A  44     -169.18   -165.76                                   
REMARK 500 21 ASP A  49     -123.60   -108.07                                   
REMARK 500 21 SER A  56      -72.27    -79.17                                   
REMARK 500 21 PRO A  58       56.09    -62.62                                   
REMARK 500 21 PRO A  72     -150.36    -59.26                                   
REMARK 500 21 ILE A  91      -40.63   -136.01                                   
REMARK 500 22 LEU A  27      -78.06    -53.60                                   
REMARK 500 22 THR A  36       65.40   -110.16                                   
REMARK 500 22 ASP A  37     -125.50   -135.81                                   
REMARK 500 22 THR A  47     -177.46    -52.91                                   
REMARK 500 22 TRP A  51       61.77   -168.06                                   
REMARK 500 22 PRO A  72       -9.30    -56.37                                   
REMARK 500 22 TYR A  87       22.34   -140.55                                   
REMARK 500 22 ILE A  91      -51.98   -139.77                                   
REMARK 500 23 ILE A  11       54.45   -143.73                                   
REMARK 500 23 ALA A  17      -70.91    -75.36                                   
REMARK 500 23 ASP A  37      -92.84   -118.43                                   
REMARK 500 23 SER A  39     -130.29    -66.43                                   
REMARK 500 23 CYS A  40      -42.90    179.00                                   
REMARK 500 23 ASP A  49     -118.63   -117.43                                   
REMARK 500 23 TRP A  51       57.88   -146.16                                   
REMARK 500 23 ASN A  75     -179.89    -67.30                                   
REMARK 500 23 ILE A  91      -57.89   -130.23                                   
REMARK 500 24 THR A  20     -112.11   -126.31                                   
REMARK 500 24 SER A  22       40.58   -106.74                                   
REMARK 500 24 ASP A  37      -84.64   -106.53                                   
REMARK 500 24 ASP A  49     -108.49   -107.60                                   
REMARK 500 24 PRO A  72      -86.61    -57.09                                   
REMARK 500 24 TYR A  86        7.03    -69.25                                   
REMARK 500 24 TYR A  87       98.06    -62.11                                   
REMARK 500 24 ASP A  88      -77.62    -87.24                                   
REMARK 500 24 LYS A  89      -66.52   -126.91                                   
REMARK 500 24 ASN A  90       84.92   -154.42                                   
REMARK 500 24 ILE A  91     -100.77   -139.39                                   
REMARK 500 25 PRO A  12       29.47    -60.91                                   
REMARK 500 25 ALA A  13     -169.64   -103.77                                   
REMARK 500 25 THR A  20     -158.12    -94.64                                   
REMARK 500 25 THR A  36       52.45   -108.30                                   
REMARK 500 25 ASP A  37      -80.16   -128.50                                   
REMARK 500 25 LYS A  38     -169.13    -76.93                                   
REMARK 500 25 SER A  39     -131.60    -74.12                                   
REMARK 500 25 CYS A  40      -44.79   -174.62                                   
REMARK 500 25 ASP A  49      -45.19   -138.10                                   
REMARK 500 25 TRP A  51       50.25   -159.47                                   
REMARK 500 25 PRO A  58       57.37    -67.53                                   
REMARK 500 25 ILE A  91      -49.19   -136.03                                   
REMARK 500 25 ALA A  96        6.63    -68.61                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 2STW   RELATED DB: PDB                                   
REMARK 900 RESTRAINED REGULARIZED MEAN STRUCTURE                                
DBREF  2STT A   10   105  UNP    P14921   ETS1_HUMAN     320    415             
DBREF  2STT B    1    17  PDB    2STT     2STT             1     17             
DBREF  2STT C   18    34  PDB    2STT     2STT            18     34             
SEQRES   1 B   17   DT  DC  DG  DA  DG  DC  DC  DG  DG  DA  DA  DG  DT          
SEQRES   2 B   17   DT  DC  DG  DA                                              
SEQRES   1 C   17   DT  DC  DG  DA  DA  DC  DT  DT  DC  DC  DG  DG  DC          
SEQRES   2 C   17   DT  DC  DG  DA                                              
SEQRES   1 A   96  VAL ILE PRO ALA ALA ALA LEU ALA GLY TYR THR GLY SER          
SEQRES   2 A   96  GLY PRO ILE GLN LEU TRP GLN PHE LEU LEU GLU LEU LEU          
SEQRES   3 A   96  THR ASP LYS SER CYS GLN SER PHE ILE SER TRP THR GLY          
SEQRES   4 A   96  ASP GLY TRP GLU PHE LYS LEU SER ASP PRO ASP GLU VAL          
SEQRES   5 A   96  ALA ARG ARG TRP GLY LYS ARG LYS ASN LYS PRO LYS MET          
SEQRES   6 A   96  ASN TYR GLU LYS LEU SER ARG GLY LEU ARG TYR TYR TYR          
SEQRES   7 A   96  ASP LYS ASN ILE ILE HIS LYS THR ALA GLY LYS ARG TYR          
SEQRES   8 A   96  VAL TYR ARG PHE VAL                                          
HELIX    1  H1 LEU A   27  THR A   36  1                                  10    
HELIX    2  H2 SER A   39  GLN A   41  5                                   3    
HELIX    3  H3 ASP A   59  LYS A   67  5                                   9    
HELIX    4  H4 TYR A   76  TYR A   85  5                                  10    
SHEET    1   A 2 SER A  45  THR A  47  0                                        
SHEET    2   A 2 GLU A  52  LYS A  54 -1  N  LYS A  54   O  SER A  45           
SHEET    1   B 2 ILE A  92  LYS A  94  0                                        
SHEET    2   B 2 TYR A 102  PHE A 104 -1  N  ARG A 103   O  HIS A  93           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system