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Database: PDB
Entry: 2W99
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Original site: 2W99 
HEADER    CELL CYCLE                              22-JAN-09   2W99              
TITLE     CRYSTAL STRUCTURE OF CDK4 IN COMPLEX WITH A D-TYPE CYCLIN             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: G1/S-SPECIFIC CYCLIN-D1;                                   
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: D-TYPE CYCLIN, PRAD1 ONCOGENE;                              
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: CELL DIVISION PROTEIN KINASE 4;                            
COMPND   8 CHAIN: B;                                                            
COMPND   9 FRAGMENT: KINASE DOMAIN, RESIDUES 1-44,48-303;                       
COMPND  10 SYNONYM: CDK4, CYCLIN-DEPENDENT KINASE 4, PSK-J3;                    
COMPND  11 EC: 2.7.11.22;                                                       
COMPND  12 ENGINEERED: YES;                                                     
COMPND  13 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;                            
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 7108;                                       
SOURCE   7 EXPRESSION_SYSTEM_CELL_LINE: SF21;                                   
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: BACULOVIRUS;                          
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;                            
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 7108;                                       
SOURCE  15 EXPRESSION_SYSTEM_CELL_LINE: SF21;                                   
SOURCE  16 EXPRESSION_SYSTEM_VECTOR_TYPE: BACULOVIRUS                           
KEYWDS    SERINE/THREONINE-PROTEIN KINASE, CHROMOSOMAL REARRANGEMENT, ATP-      
KEYWDS   2 BINDING, TRANSFERASE, POLYMORPHISM, CELL DIVISION, PROTO-ONCOGENE,   
KEYWDS   3 PHOSPHOPROTEIN, DISEASE MUTATION, NUCLEOTIDE-BINDING, CYCLIN         
KEYWDS   4 DEPENDENT KINASE, KINASE, CYCLIN, ONCOLOGY, CELL CYCLE, DRUG DESGN   
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    P.J.DAY,A.CLEASBY,I.J.TICKLE,M.O.REILLY,J.E.COYLE,F.P.HOLDING,        
AUTHOR   2 R.L.MCMENAMIN,J.YON,R.CHOPRA,C.LENGAUER,H.JHOTI                      
REVDAT   3   24-APR-19 2W99    1       SOURCE                                   
REVDAT   2   31-MAR-09 2W99    1       JRNL                                     
REVDAT   1   10-MAR-09 2W99    0                                                
JRNL        AUTH   P.J.DAY,A.CLEASBY,I.J.TICKLE,M.O'REILLY,J.E.COYLE,           
JRNL        AUTH 2 F.P.HOLDING,R.L.MCMENAMIN,J.YON,R.CHOPRA,C.LENGAUER,H.JHOTI  
JRNL        TITL   CRYSTAL STRUCTURE OF HUMAN CDK4 IN COMPLEX WITH A D-TYPE     
JRNL        TITL 2 CYCLIN.                                                      
JRNL        REF    PROC.NATL.ACAD.SCI.USA        V. 106  4166 2009              
JRNL        REFN                   ISSN 0027-8424                               
JRNL        PMID   19237565                                                     
JRNL        DOI    10.1073/PNAS.0809645106                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.80 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : BUSTER-TNT 2.1.1                                     
REMARK   3   AUTHORS     : BRICOGNE,BLANC,BRANDL,FLENSBURG,KELLER,              
REMARK   3               : PACIOREK,ROVERSI,SMART,VONRHEIN,WOMACK,              
REMARK   3               : MATTHEWS,TEN EYCK,TRONRUD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.80                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 94.49                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 17640                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD           : THROUGHOUT                     
REMARK   3   FREE R VALUE TEST SET SELECTION   : RANDOM                         
REMARK   3   R VALUE     (WORKING + TEST SET)  : 0.216                          
REMARK   3   R VALUE            (WORKING SET)  : 0.213                          
REMARK   3   FREE R VALUE                      : 0.270                          
REMARK   3   FREE R VALUE TEST SET SIZE   (%)  : 4.790                          
REMARK   3   FREE R VALUE TEST SET COUNT       : 845                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE   : NULL                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED               : 9                        
REMARK   3   BIN RESOLUTION RANGE HIGH   (ANGSTROMS) : 2.80                     
REMARK   3   BIN RESOLUTION RANGE LOW    (ANGSTROMS) : 2.97                     
REMARK   3   BIN COMPLETENESS (WORKING+TEST)     (%) : 98.27                    
REMARK   3   REFLECTIONS IN BIN (WORKING + TEST SET) : 2656                     
REMARK   3   BIN R VALUE        (WORKING + TEST SET) : 0.2166                   
REMARK   3   REFLECTIONS IN BIN        (WORKING SET) : 2524                     
REMARK   3   BIN R VALUE               (WORKING SET) : 0.2142                   
REMARK   3   BIN FREE R VALUE                        : 0.2633                   
REMARK   3   BIN FREE R VALUE TEST SET SIZE      (%) : 4.97                     
REMARK   3   BIN FREE R VALUE TEST SET COUNT         : 132                      
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE     : NULL                     
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 4259                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 126                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 78.76                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 86.21                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -4.53183                                             
REMARK   3    B22 (A**2) : 10.15250                                             
REMARK   3    B33 (A**2) : -5.62067                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT                    (A) : NULL                
REMARK   3   DPI (BLOW EQ-10) BASED ON R VALUE        (A) : NULL                
REMARK   3   DPI (BLOW EQ-9) BASED ON FREE R VALUE    (A) : NULL                
REMARK   3   DPI (CRUICKSHANK) BASED ON R VALUE       (A) : NULL                
REMARK   3   DPI (CRUICKSHANK) BASED ON FREE R VALUE  (A) : NULL                
REMARK   3                                                                      
REMARK   3   REFERENCES: BLOW, D. (2002) ACTA CRYST D58, 792-797                
REMARK   3               CRUICKSHANK, D.W.J. (1999) ACTA CRYST D55, 583-601     
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : NULL                          
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : NULL                          
REMARK   3                                                                      
REMARK   3   NUMBER OF GEOMETRIC FUNCTION TERMS DEFINED : 15                    
REMARK   3   TERM                          COUNT    WEIGHT   FUNCTION.          
REMARK   3    BOND LENGTHS              : NULL   ; NULL   ; NULL                
REMARK   3    BOND ANGLES               : NULL   ; NULL   ; NULL                
REMARK   3    TORSION ANGLES            : NULL   ; NULL   ; NULL                
REMARK   3    TRIGONAL CARBON PLANES    : NULL   ; NULL   ; NULL                
REMARK   3    GENERAL PLANES            : NULL   ; NULL   ; NULL                
REMARK   3    ISOTROPIC THERMAL FACTORS : NULL   ; NULL   ; NULL                
REMARK   3    BAD NON-BONDED CONTACTS   : NULL   ; NULL   ; NULL                
REMARK   3    IMPROPER TORSIONS         : NULL   ; NULL   ; NULL                
REMARK   3    PSEUDOROTATION ANGLES     : NULL   ; NULL   ; NULL                
REMARK   3    CHIRAL IMPROPER TORSION   : NULL   ; NULL   ; NULL                
REMARK   3    SUM OF OCCUPANCIES        : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY DISTANCES         : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY ANGLES            : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY TORSION           : NULL   ; NULL   ; NULL                
REMARK   3    IDEAL-DIST CONTACT TERM   : NULL   ; NULL   ; NULL                
REMARK   3                                                                      
REMARK   3   RMS DEVIATIONS FROM IDEAL VALUES.                                  
REMARK   3    BOND LENGTHS                       (A) : NULL                     
REMARK   3    BOND ANGLES                  (DEGREES) : NULL                     
REMARK   3    PEPTIDE OMEGA TORSION ANGLES (DEGREES) : NULL                     
REMARK   3    OTHER TORSION ANGLES         (DEGREES) : NULL                     
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING   
REMARK   3  POSITIONS. DISORDERED REGIONS WERE MODELLED STEREOCHEMICALLY        
REMARK   3  WHERE THE DENSITY WAS NOT CLEAR. IN REGIONS WHERE THE DENSITY       
REMARK   3  WAS UNINTERPRETABLE OR ABSENT, RESIDUES WERE OMITTED.               
REMARK   4                                                                      
REMARK   4 2W99 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 22-JAN-09.                  
REMARK 100 THE DEPOSITION ID IS D_1290038619.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 13-DEC-06                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID29                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.94                               
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC CCD                           
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM                             
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 17640                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.800                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 94.500                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.6                               
REMARK 200  DATA REDUNDANCY                : 4.500                              
REMARK 200  R MERGE                    (I) : 0.09000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 6.0000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.80                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.91                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 94.8                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.50                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.54000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.500                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1BLX                                       
REMARK 200                                                                      
REMARK 200 REMARK: NONE                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 56.28                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.84                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: NULL                                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       28.51150            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       94.37350            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       32.34200            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       94.37350            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       28.51150            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       32.34200            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PQS                                                   
REMARK 350 TOTAL BURIED SURFACE AREA: 2420 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 30860 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -11.9 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 400                                                                      
REMARK 400 COMPOUND                                                             
REMARK 400 ENGINEERED RESIDUE IN CHAIN B, GLY 43 TO GLU                         
REMARK 400 ENGINEERED RESIDUE IN CHAIN B, GLY 44 TO GLU                         
REMARK 400 ENGINEERED RESIDUE IN CHAIN B, THR 172 TO ALA                        
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     GLU A     2                                                      
REMARK 465     HIS A     3                                                      
REMARK 465     GLN A     4                                                      
REMARK 465     LEU A     5                                                      
REMARK 465     LEU A     6                                                      
REMARK 465     CYS A     7                                                      
REMARK 465     CYS A     8                                                      
REMARK 465     GLU A     9                                                      
REMARK 465     VAL A    10                                                      
REMARK 465     GLU A    11                                                      
REMARK 465     THR A    12                                                      
REMARK 465     ILE A    13                                                      
REMARK 465     ARG A    14                                                      
REMARK 465     ARG A    15                                                      
REMARK 465     ALA A    16                                                      
REMARK 465     TYR A    17                                                      
REMARK 465     PRO A    18                                                      
REMARK 465     ASP A    19                                                      
REMARK 465     ALA A    20                                                      
REMARK 465     ASN A    21                                                      
REMARK 465     PRO A   268                                                      
REMARK 465     LYS A   269                                                      
REMARK 465     ALA A   270                                                      
REMARK 465     ALA A   271                                                      
REMARK 465     MET B     1                                                      
REMARK 465     HIS B   296                                                      
REMARK 465     LYS B   297                                                      
REMARK 465     ASP B   298                                                      
REMARK 465     GLU B   299                                                      
REMARK 465     GLY B   300                                                      
REMARK 465     ASN B   301                                                      
REMARK 465     PRO B   302                                                      
REMARK 465     GLU B   303                                                      
REMARK 465     HIS B   304                                                      
REMARK 465     HIS B   305                                                      
REMARK 465     HIS B   306                                                      
REMARK 465     HIS B   307                                                      
REMARK 465     HIS B   308                                                      
REMARK 465     HIS B   309                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A  72       49.16     38.28                                   
REMARK 500    ASN A 222      -58.10   -137.51                                   
REMARK 500    PHE A 223       46.02    -94.21                                   
REMARK 500    LEU A 224       23.60    -70.87                                   
REMARK 500    ARG A 228       53.13     36.63                                   
REMARK 500    LYS A 238       71.40     53.29                                   
REMARK 500    SER A 257       -5.12    -56.94                                   
REMARK 500    GLN A 264        7.18    -66.54                                   
REMARK 500    MET A 266      -72.48    -68.59                                   
REMARK 500    ARG B   5       50.99    -63.41                                   
REMARK 500    VAL B   9     -122.21   -140.01                                   
REMARK 500    ALA B  10      179.71    -41.81                                   
REMARK 500    ILE B  12       -4.76    -56.69                                   
REMARK 500    ALA B  16      -52.45    -11.94                                   
REMARK 500    VAL B  20       73.22   -110.28                                   
REMARK 500    LEU B  34       99.27    -66.48                                   
REMARK 500    LEU B  63      -43.13   -162.88                                   
REMARK 500    ASP B  84      101.40      0.76                                   
REMARK 500    ARG B  85        2.91     82.82                                   
REMARK 500    THR B 102      -13.99    -47.84                                   
REMARK 500    LYS B 106       53.44    -67.95                                   
REMARK 500    LEU B 112     -165.72   -104.81                                   
REMARK 500    CYS B 135       12.17     80.08                                   
REMARK 500    ARG B 139      -72.61    -89.35                                   
REMARK 500    ASN B 145       38.24    -92.75                                   
REMARK 500    SER B 150     -175.48    178.48                                   
REMARK 500    THR B 153      -60.07   -152.03                                   
REMARK 500    VAL B 154       51.78     74.49                                   
REMARK 500    ASP B 158       20.39     48.57                                   
REMARK 500    PHE B 159      153.47    -49.30                                   
REMARK 500    LEU B 161      -34.58    -10.45                                   
REMARK 500    MET B 169       14.48    -66.29                                   
REMARK 500    PRO B 173     -164.15   -106.11                                   
REMARK 500    VAL B 174      -70.20   -138.18                                   
REMARK 500    VAL B 176       34.59     70.09                                   
REMARK 500    THR B 177       59.54    -58.25                                   
REMARK 500    LEU B 178       50.76    -67.38                                   
REMARK 500    PRO B 183       -9.91    -56.65                                   
REMARK 500    LEU B 186       17.57   -143.54                                   
REMARK 500    GLN B 188     -123.66   -133.78                                   
REMARK 500    SER B 189       56.57   -169.69                                   
REMARK 500    SER B 199      -17.11    -48.67                                   
REMARK 500    ARG B 209       30.40   -147.60                                   
REMARK 500    ARG B 210     -118.47   -119.88                                   
REMARK 500    LYS B 211     -167.28   -123.49                                   
REMARK 500    PHE B 214       28.66   -146.36                                   
REMARK 500    ASN B 217      -79.63   -176.63                                   
REMARK 500    ASP B 241      -23.80   -151.74                                   
REMARK 500    SER B 243       69.63     75.28                                   
REMARK 500    PHE B 249      -63.77   -103.01                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      56 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH A2005        DISTANCE =  6.32 ANGSTROMS                       
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1LD2   RELATED DB: PDB                                   
REMARK 900 THEORETICAL STRUCTURE OF HUMAN CYCLIN- DEPENDENT KINASE 4(CDK4)      
REMARK 900 RELATED ID: 2W96   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF CDK4 IN COMPLEX WITH A D-TYPE CYCLIN            
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999 RESIDUES 1-271 WERE EXPRESSED, BUT THE N AND C-TERMINAL              
REMARK 999 WERE DISORDERED AND THEREFORE NOT COMPLETE IN THE MODEL.             
REMARK 999 HIS TAG WAS ADDED AT THE C-TERMINAL. RESIDUES 45, 46, 47             
REMARK 999 IN CHAIN B WERE DELETED.                                             
DBREF  2W99 A    1   271  UNP    P24385   CCND1_HUMAN      1    271             
DBREF  2W99 B    1    44  UNP    P11802   CDK4_HUMAN       1     44             
DBREF  2W99 B   48   303  UNP    P11802   CDK4_HUMAN      48    303             
DBREF  2W99 B  304   309  PDB    2W99     2W99           304    309             
SEQADV 2W99 GLU B   43  UNP  P11802    GLY    43 ENGINEERED MUTATION            
SEQADV 2W99 GLU B   44  UNP  P11802    GLY    44 ENGINEERED MUTATION            
SEQADV 2W99 ALA B  172  UNP  P11802    THR   172 ENGINEERED MUTATION            
SEQRES   1 A  271  MET GLU HIS GLN LEU LEU CYS CYS GLU VAL GLU THR ILE          
SEQRES   2 A  271  ARG ARG ALA TYR PRO ASP ALA ASN LEU LEU ASN ASP ARG          
SEQRES   3 A  271  VAL LEU ARG ALA MET LEU LYS ALA GLU GLU THR CYS ALA          
SEQRES   4 A  271  PRO SER VAL SER TYR PHE LYS CYS VAL GLN LYS GLU VAL          
SEQRES   5 A  271  LEU PRO SER MET ARG LYS ILE VAL ALA THR TRP MET LEU          
SEQRES   6 A  271  GLU VAL CYS GLU GLU GLN LYS CYS GLU GLU GLU VAL PHE          
SEQRES   7 A  271  PRO LEU ALA MET ASN TYR LEU ASP ARG PHE LEU SER LEU          
SEQRES   8 A  271  GLU PRO VAL LYS LYS SER ARG LEU GLN LEU LEU GLY ALA          
SEQRES   9 A  271  THR CYS MET PHE VAL ALA SER LYS MET LYS GLU THR ILE          
SEQRES  10 A  271  PRO LEU THR ALA GLU LYS LEU CYS ILE TYR THR ASP ASN          
SEQRES  11 A  271  SER ILE ARG PRO GLU GLU LEU LEU GLN MET GLU LEU LEU          
SEQRES  12 A  271  LEU VAL ASN LYS LEU LYS TRP ASN LEU ALA ALA MET THR          
SEQRES  13 A  271  PRO HIS ASP PHE ILE GLU HIS PHE LEU SER LYS MET PRO          
SEQRES  14 A  271  GLU ALA GLU GLU ASN LYS GLN ILE ILE ARG LYS HIS ALA          
SEQRES  15 A  271  GLN THR PHE VAL ALA LEU CYS ALA THR ASP VAL LYS PHE          
SEQRES  16 A  271  ILE SER ASN PRO PRO SER MET VAL ALA ALA GLY SER VAL          
SEQRES  17 A  271  VAL ALA ALA VAL GLN GLY LEU ASN LEU ARG SER PRO ASN          
SEQRES  18 A  271  ASN PHE LEU SER TYR TYR ARG LEU THR ARG PHE LEU SER          
SEQRES  19 A  271  ARG VAL ILE LYS CYS ASP PRO ASP CYS LEU ARG ALA CYS          
SEQRES  20 A  271  GLN GLU GLN ILE GLU ALA LEU LEU GLU SER SER LEU ARG          
SEQRES  21 A  271  GLN ALA GLN GLN ASN MET ASP PRO LYS ALA ALA                  
SEQRES   1 B  306  MET ALA THR SER ARG TYR GLU PRO VAL ALA GLU ILE GLY          
SEQRES   2 B  306  VAL GLY ALA TYR GLY THR VAL TYR LYS ALA ARG ASP PRO          
SEQRES   3 B  306  HIS SER GLY HIS PHE VAL ALA LEU LYS SER VAL ARG VAL          
SEQRES   4 B  306  PRO ASN GLY GLU GLU GLY LEU PRO ILE SER THR VAL ARG          
SEQRES   5 B  306  GLU VAL ALA LEU LEU ARG ARG LEU GLU ALA PHE GLU HIS          
SEQRES   6 B  306  PRO ASN VAL VAL ARG LEU MET ASP VAL CYS ALA THR SER          
SEQRES   7 B  306  ARG THR ASP ARG GLU ILE LYS VAL THR LEU VAL PHE GLU          
SEQRES   8 B  306  HIS VAL ASP GLN ASP LEU ARG THR TYR LEU ASP LYS ALA          
SEQRES   9 B  306  PRO PRO PRO GLY LEU PRO ALA GLU THR ILE LYS ASP LEU          
SEQRES  10 B  306  MET ARG GLN PHE LEU ARG GLY LEU ASP PHE LEU HIS ALA          
SEQRES  11 B  306  ASN CYS ILE VAL HIS ARG ASP LEU LYS PRO GLU ASN ILE          
SEQRES  12 B  306  LEU VAL THR SER GLY GLY THR VAL LYS LEU ALA ASP PHE          
SEQRES  13 B  306  GLY LEU ALA ARG ILE TYR SER TYR GLN MET ALA LEU ALA          
SEQRES  14 B  306  PRO VAL VAL VAL THR LEU TRP TYR ARG ALA PRO GLU VAL          
SEQRES  15 B  306  LEU LEU GLN SER THR TYR ALA THR PRO VAL ASP MET TRP          
SEQRES  16 B  306  SER VAL GLY CYS ILE PHE ALA GLU MET PHE ARG ARG LYS          
SEQRES  17 B  306  PRO LEU PHE CYS GLY ASN SER GLU ALA ASP GLN LEU GLY          
SEQRES  18 B  306  LYS ILE PHE ASP LEU ILE GLY LEU PRO PRO GLU ASP ASP          
SEQRES  19 B  306  TRP PRO ARG ASP VAL SER LEU PRO ARG GLY ALA PHE PRO          
SEQRES  20 B  306  PRO ARG GLY PRO ARG PRO VAL GLN SER VAL VAL PRO GLU          
SEQRES  21 B  306  MET GLU GLU SER GLY ALA GLN LEU LEU LEU GLU MET LEU          
SEQRES  22 B  306  THR PHE ASN PRO HIS LYS ARG ILE SER ALA PHE ARG ALA          
SEQRES  23 B  306  LEU GLN HIS SER TYR LEU HIS LYS ASP GLU GLY ASN PRO          
SEQRES  24 B  306  GLU HIS HIS HIS HIS HIS HIS                                  
FORMUL   3  HOH   *126(H2 O)                                                    
HELIX    1   1 LEU A   22  CYS A   38  1                                  17    
HELIX    2   2 SER A   43  VAL A   48  1                                   6    
HELIX    3   3 LEU A   53  GLN A   71  1                                  19    
HELIX    4   4 GLU A   76  SER A   90  1                                  15    
HELIX    5   5 LYS A   95  SER A   97  5                                   3    
HELIX    6   6 ARG A   98  GLU A  115  1                                  18    
HELIX    7   7 THR A  120  TYR A  127  1                                   8    
HELIX    8   8 ARG A  133  LEU A  148  1                                  16    
HELIX    9   9 THR A  156  MET A  168  1                                  13    
HELIX   10  10 ALA A  171  THR A  191  1                                  21    
HELIX   11  11 VAL A  193  ASN A  198  1                                   6    
HELIX   12  12 PRO A  199  SER A  219  1                                  21    
HELIX   13  13 ARG A  228  LYS A  238  1                                  11    
HELIX   14  14 ASP A  240  ALA A  262  1                                  23    
HELIX   15  15 PRO B   50  ARG B   62  1                                  13    
HELIX   16  16 LEU B  100  LYS B  106  1                                   7    
HELIX   17  17 ILE B  117  ASN B  134  1                                  18    
HELIX   18  18 TYR B  165  LEU B  171  5                                   7    
HELIX   19  19 THR B  193  MET B  207  1                                  15    
HELIX   20  20 SER B  218  GLY B  231  1                                  14    
HELIX   21  21 PRO B  256  VAL B  261  1                                   6    
HELIX   22  22 GLU B  265  LEU B  276  1                                  12    
HELIX   23  23 ARG B  288  HIS B  292  5                                   5    
SHEET    1  BA 3 GLU B   7  PRO B   8  0                                        
SHEET    2  BA 3 ALA B  23  ARG B  24 -1  O  ARG B  24   N  GLU B   7           
SHEET    3  BA 3 PHE B  31  VAL B  32 -1  O  VAL B  32   N  ALA B  23           
SHEET    1  BB 2 VAL B  14  GLY B  15  0                                        
SHEET    2  BB 2 GLY B  18  THR B  19 -1  O  GLY B  18   N  GLY B  15           
SHEET    1  BC 3 ARG B  38  VAL B  39  0                                        
SHEET    2  BC 3 GLU B  86  PHE B  93 -1  O  ILE B  87   N  VAL B  39           
SHEET    3  BC 3 LEU B  74  THR B  83 -1  O  ASP B  76   N  VAL B  92           
SHEET    1  BD 2 GLN B  98  ASP B  99  0                                        
SHEET    2  BD 2 LEU B 147  VAL B 148 -1  O  VAL B 148   N  GLN B  98           
CISPEP   1 ALA B    2    THR B    3          0        -2.61                     
CISPEP   2 LEU B  112    PRO B  113          0        -5.37                     
CISPEP   3 PRO B  173    VAL B  174          0        -8.85                     
CISPEP   4 PHE B  208    ARG B  209          0        -7.60                     
CISPEP   5 GLU B  235    ASP B  236          0        11.58                     
CISPEP   6 ARG B  240    ASP B  241          0         7.71                     
CISPEP   7 PRO B  262    GLU B  263          0        -5.94                     
CRYST1   57.023   64.684  188.747  90.00  90.00  90.00 P 21 21 21    4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.017537  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.015460  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.005298        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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