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Database: PDB
Entry: 2YUM
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Original site: 2YUM 
HEADER    TRANSCRIPTION                           06-APR-07   2YUM              
TITLE     SOLUTION STRUCTURE OF THE MYB-LIKE DNA-BINDING DOMAIN OF              
TITLE    2 HUMAN ZZZ3 PROTEIN                                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ZINC FINGER ZZ-TYPE-CONTAINING PROTEIN 3;                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: MYB-LIKE DNA-BINDING DOMAIN, UNP RESIDUES 652-             
COMPND   5 713;                                                                 
COMPND   6 SYNONYM: ZZZ3 PROTEIN;                                               
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ZZZ3;                                                          
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P050613-19;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    TRANSCRIPTION, STRUCTURAL GENOMICS, NPPSFA, NATIONAL                  
KEYWDS   2 PROJECT ON PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES,               
KEYWDS   3 RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI                
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    H.ABE,N.TOCHIO,K.MIYAMOTO,K.SAITO,S.KOSHIBA,M.INOUE,                  
AUTHOR   2 T.KIGAWA,S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS             
AUTHOR   3 INITIATIVE (RSGI)                                                    
REVDAT   2   24-FEB-09 2YUM    1       VERSN                                    
REVDAT   1   09-OCT-07 2YUM    0                                                
JRNL        AUTH   H.ABE,N.TOCHIO,K.MIYAMOTO,K.SAITO,S.KOSHIBA,                 
JRNL        AUTH 2 M.INOUE,T.KIGAWA,S.YOKOYAMA                                  
JRNL        TITL   SOLUTION STRUCTURE OF THE MYB-LIKE DNA-BINDING               
JRNL        TITL 2 DOMAIN OF HUMAN ZZZ3 PROTEIN                                 
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2YUM COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 03-JUL-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB027154.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.14MM PROTEIN; 20MM D-TRIS-       
REMARK 210                                   HCL; 100MM NACL; 1MM D-DTT;        
REMARK 210                                   0.02% NAN3; 90% H2O, 10% D2O       
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 3.5, NMRPIPE               
REMARK 210                                   20031121, NMNMRVIEW 5.0.4,         
REMARK 210                                   KUJIRA 0.9747, CYANA 2.0.17        
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS, TARGET       
REMARK 210                                   FUNCTION                           
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 TRP A  11      172.44    -49.03                                   
REMARK 500  1 LYS A  61       39.45    -82.38                                   
REMARK 500  1 LEU A  62      -33.06   -132.16                                   
REMARK 500  1 THR A  63       72.22    -66.70                                   
REMARK 500  1 LYS A  64       36.16     34.40                                   
REMARK 500  1 PRO A  72       97.71    -69.83                                   
REMARK 500  2 SER A   5      -60.85    -94.52                                   
REMARK 500  2 LYS A  61      -35.22    -34.37                                   
REMARK 500  3 LYS A  49      -70.04    -58.85                                   
REMARK 500  3 LYS A  64      129.99   -173.33                                   
REMARK 500  3 ILE A  67      137.84    -34.96                                   
REMARK 500  3 PRO A  72       94.78    -69.77                                   
REMARK 500  4 GLN A   9       42.29   -100.62                                   
REMARK 500  4 GLU A  32       39.31    -82.31                                   
REMARK 500  4 SER A  33      -55.11   -128.36                                   
REMARK 500  4 LYS A  49      -70.00    -56.97                                   
REMARK 500  4 GLN A  50      -38.18    -37.04                                   
REMARK 500  4 THR A  63       40.13    -81.78                                   
REMARK 500  4 ALA A  65       43.51     34.52                                   
REMARK 500  4 VAL A  69      -60.53    -92.39                                   
REMARK 500  4 PRO A  72       92.03    -69.73                                   
REMARK 500  5 SER A   2       42.09    -85.15                                   
REMARK 500  5 PHE A  59      -52.48   -132.28                                   
REMARK 500  5 LEU A  62       48.65   -101.21                                   
REMARK 500  5 LYS A  64      -63.52   -129.46                                   
REMARK 500  5 SER A  74      100.51    -54.14                                   
REMARK 500  6 SER A   2      150.99    -41.60                                   
REMARK 500  6 GLN A   9       36.83    -85.78                                   
REMARK 500  6 TRP A  11      116.26    -37.10                                   
REMARK 500  6 GLN A  50      -39.88    -39.22                                   
REMARK 500  6 LYS A  64       49.59     34.64                                   
REMARK 500  6 ALA A  65      150.21    -34.42                                   
REMARK 500  6 PRO A  72       86.47    -69.78                                   
REMARK 500  7 GLN A   9       42.15    -89.44                                   
REMARK 500  7 TRP A  11      155.19    -38.35                                   
REMARK 500  7 LYS A  49      -71.28    -61.08                                   
REMARK 500  7 GLN A  50      -29.42    -38.46                                   
REMARK 500  7 THR A  63      159.27    -45.83                                   
REMARK 500  7 PRO A  72       89.54    -69.71                                   
REMARK 500  8 SER A   3       42.19    -90.86                                   
REMARK 500  8 SER A   5       41.79     39.34                                   
REMARK 500  8 ALA A  48      -26.68    -38.18                                   
REMARK 500  8 PRO A  68     -173.29    -69.76                                   
REMARK 500  8 SER A  70      139.91   -172.20                                   
REMARK 500  9 SER A   3      -62.29   -104.08                                   
REMARK 500  9 ASN A   8      -47.78   -133.16                                   
REMARK 500  9 GLN A  16      -70.20    -45.47                                   
REMARK 500  9 THR A  63      144.27    -39.60                                   
REMARK 500  9 LYS A  64      134.55    -39.33                                   
REMARK 500  9 SER A  70      -46.68   -134.77                                   
REMARK 500 10 ASN A   8       79.78   -104.45                                   
REMARK 500 10 TRP A  11      171.87    -51.70                                   
REMARK 500 10 GLN A  50      -39.29    -39.01                                   
REMARK 500 10 THR A  63      172.63    -51.32                                   
REMARK 500 10 ILE A  67      132.84    -33.72                                   
REMARK 500 11 ASN A   8       45.43    -91.59                                   
REMARK 500 11 PHE A  59      -56.14   -131.67                                   
REMARK 500 11 LEU A  62       45.99   -107.84                                   
REMARK 500 11 THR A  63      -62.38    -91.19                                   
REMARK 500 12 GLN A   9       66.90   -108.69                                   
REMARK 500 12 ALA A  48      -28.34    -37.15                                   
REMARK 500 12 LYS A  49      -71.00    -74.76                                   
REMARK 500 12 VAL A  69      140.06    -35.17                                   
REMARK 500 13 SER A   5       42.63     38.63                                   
REMARK 500 13 LYS A  18      -36.90    -39.40                                   
REMARK 500 13 GLU A  32      -29.93    -36.39                                   
REMARK 500 13 SER A  33      -70.65    -50.47                                   
REMARK 500 13 LYS A  49      -70.10    -54.66                                   
REMARK 500 13 GLN A  50      -36.63    -38.16                                   
REMARK 500 14 SER A   3      -53.70   -122.56                                   
REMARK 500 14 SER A   6      -53.51   -131.99                                   
REMARK 500 14 LYS A  49      -70.23    -58.28                                   
REMARK 500 14 ILE A  67      132.82    -33.61                                   
REMARK 500 15 SER A   2       42.09   -100.61                                   
REMARK 500 15 VAL A  13      -72.57    -33.73                                   
REMARK 500 15 LEU A  62      -33.39    -36.43                                   
REMARK 500 15 ALA A  65       41.97    -80.56                                   
REMARK 500 15 SER A  74      147.58   -172.22                                   
REMARK 500 16 ASN A   8       44.71    -81.99                                   
REMARK 500 16 VAL A  69       39.93     29.57                                   
REMARK 500 16 SER A  70      150.79    -34.45                                   
REMARK 500 16 PRO A  72     -177.32    -69.81                                   
REMARK 500 17 LEU A  62       33.35    -88.19                                   
REMARK 500 17 LYS A  64      161.56    -41.88                                   
REMARK 500 17 ALA A  65      155.39    -43.70                                   
REMARK 500 17 SER A  70       47.16     39.61                                   
REMARK 500 17 SER A  74      -59.43   -129.95                                   
REMARK 500 18 VAL A  13      -37.10    -36.65                                   
REMARK 500 18 VAL A  69      143.22    -37.37                                   
REMARK 500 19 TRP A  11      134.49    -34.05                                   
REMARK 500 19 GLU A  20      -39.17    -34.67                                   
REMARK 500 19 LYS A  49      -71.28    -60.91                                   
REMARK 500 19 THR A  63      149.81    -34.85                                   
REMARK 500 19 SER A  73      -71.97    -73.92                                   
REMARK 500 20 GLN A   9       41.75    -87.90                                   
REMARK 500 20 TRP A  11      137.64    -39.28                                   
REMARK 500 20 LYS A  49      -70.37    -69.14                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSI002027679.2   RELATED DB: TARGETDB                    
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999 THE SEQUENCE IS BASED ON REFERENCE 2 IN THE                          
REMARK 999 DATABASE, ZZZ3_HUMAN.                                                
DBREF  2YUM A    8    69  UNP    Q8IYH5   ZZZ3_HUMAN     652    713             
SEQADV 2YUM GLY A    1  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A    2  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A    3  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM GLY A    4  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A    5  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A    6  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM GLY A    7  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A   70  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM GLY A   71  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM PRO A   72  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A   73  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM SER A   74  UNP  Q8IYH5              EXPRESSION TAG                 
SEQADV 2YUM GLY A   75  UNP  Q8IYH5              EXPRESSION TAG                 
SEQRES   1 A   75  GLY SER SER GLY SER SER GLY ASN GLN LEU TRP THR VAL          
SEQRES   2 A   75  GLU GLU GLN LYS LYS LEU GLU GLN LEU LEU ILE LYS TYR          
SEQRES   3 A   75  PRO PRO GLU GLU VAL GLU SER ARG ARG TRP GLN LYS ILE          
SEQRES   4 A   75  ALA ASP GLU LEU GLY ASN ARG THR ALA LYS GLN VAL ALA          
SEQRES   5 A   75  SER GLN VAL GLN LYS TYR PHE ILE LYS LEU THR LYS ALA          
SEQRES   6 A   75  GLY ILE PRO VAL SER GLY PRO SER SER GLY                      
HELIX    1   1 THR A   12  TYR A   26  1                                  15    
HELIX    2   2 VAL A   31  GLY A   44  1                                  14    
HELIX    3   3 THR A   47  ILE A   60  1                                  14    
HELIX    4   4 LYS A   61  THR A   63  5                                   3    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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