GenomeNet

Database: PDB
Entry: 3IOZ
LinkDB: 3IOZ
Original site: 3IOZ 
HEADER    VIRAL PROTEIN/SIGNALING PROTEIN         15-AUG-09   3IOZ              
TITLE     SIVMAC239 NEF IN COMPLEX WITH A TCR ZETA POLYPEPTIDE DP1 (L51-D93)    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN NEF;                                               
COMPND   3 CHAIN: A;                                                            
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: T-CELL SURFACE GLYCOPROTEIN CD3 ZETA CHAIN;                
COMPND   7 CHAIN: B;                                                            
COMPND   8 SYNONYM: T-CELL RECEPTOR T3 ZETA CHAIN;                              
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SIMIAN IMMUNODEFICIENCY VIRUS;                  
SOURCE   3 ORGANISM_TAXID: 388909;                                              
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   6 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3);                                
SOURCE   7 MOL_ID: 2;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   9 ORGANISM_COMMON: HUMAN;                                              
SOURCE  10 ORGANISM_TAXID: 9606;                                                
SOURCE  11 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  12 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  13 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3)                                 
KEYWDS    PROTEIN-PROTEIN COMPLEX, CELL MEMBRANE, LIPOPROTEIN, MEMBRANE,        
KEYWDS   2 MYRISTATE, VIRAL IMMUNOEVASION, VIRULENCE, DISULFIDE BOND, HOST-     
KEYWDS   3 VIRUS INTERACTION, PHOSPHOPROTEIN, RECEPTOR, TRANSMEMBRANE, VIRAL    
KEYWDS   4 PROTEIN-ANTIVIRAL PROTEIN COMPLEX, VIRAL PROTEIN-SIGNALING PROTEIN   
KEYWDS   5 COMPLEX                                                              
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    W.M.KIM,A.B.SIGALOV,L.J.STERN                                         
REVDAT   3   01-NOV-17 3IOZ    1       REMARK                                   
REVDAT   2   28-MAR-12 3IOZ    1       JRNL   VERSN                             
REVDAT   1   02-FEB-10 3IOZ    0                                                
JRNL        AUTH   W.M.KIM,A.B.SIGALOV,L.J.STERN                                
JRNL        TITL   PSEUDO-MEROHEDRAL TWINNING AND NONCRYSTALLOGRAPHIC SYMMETRY  
JRNL        TITL 2 IN ORTHORHOMBIC CRYSTALS OF SIVMAC239 NEF CORE DOMAIN BOUND  
JRNL        TITL 3 TO DIFFERENT-LENGTH TCRZETA FRAGMENTS.                       
JRNL        REF    ACTA CRYSTALLOGR.,SECT.D      V.  66   163 2010              
JRNL        REFN                   ISSN 0907-4449                               
JRNL        PMID   20124696                                                     
JRNL        DOI    10.1107/S090744490904880X                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.70 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX                                               
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.70                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 35.85                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 0.220                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 94.4                           
REMARK   3   NUMBER OF REFLECTIONS             : 2923                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.302                           
REMARK   3   R VALUE            (WORKING SET) : 0.301                           
REMARK   3   FREE R VALUE                     : 0.329                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.380                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 128                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1  0.0000 -  3.6990    0.94     2795   128  0.3010 0.3290        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : 0.31                                          
REMARK   3   B_SOL              : 143.6                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 2.170            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : NULL             
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 206.4                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 28.37900                                             
REMARK   3    B22 (A**2) : 28.37900                                             
REMARK   3    B33 (A**2) : -84.59200                                            
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.003           1088                                  
REMARK   3   ANGLE     :  0.565           1478                                  
REMARK   3   CHIRALITY :  0.040            144                                  
REMARK   3   PLANARITY :  0.003            187                                  
REMARK   3   DIHEDRAL  : 13.315            383                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3IOZ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 19-AUG-09.                  
REMARK 100 THE DEPOSITION ID IS D_1000054683.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 18-OCT-08                          
REMARK 200  TEMPERATURE           (KELVIN) : NULL                               
REMARK 200  PH                             : 8.2                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : NSLS                               
REMARK 200  BEAMLINE                       : X29A                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.290                              
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO, HKL-2000                    
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK, HKL-2000                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 3122                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.700                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.4                               
REMARK 200  DATA REDUNDANCY                : 12.60                              
REMARK 200  R MERGE                    (I) : 0.05100                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 12.8000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.70                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.83                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 12.70                              
REMARK 200  R MERGE FOR SHELL          (I) : 0.39300                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER 2.1.4                                          
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 57.19                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.87                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 15% PEG 3350, 0.15M KF, 0.1M HEPES, PH   
REMARK 280  8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+3/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+3/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       94.72450            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       25.81900            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       25.81900            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000      142.08675            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       25.81900            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       25.81900            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       47.36225            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       25.81900            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       25.81900            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000      142.08675            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       25.81900            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       25.81900            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       47.36225            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       94.72450            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1040 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 7380 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -6.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    93                                                      
REMARK 465     SER A    94                                                      
REMARK 465     ASP A    95                                                      
REMARK 465     ASP A    96                                                      
REMARK 465     LEU A    97                                                      
REMARK 465     VAL A    98                                                      
REMARK 465     GLY A    99                                                      
REMARK 465     VAL A   100                                                      
REMARK 465     SER A   101                                                      
REMARK 465     VAL A   102                                                      
REMARK 465     ARG A   103                                                      
REMARK 465     PRO A   104                                                      
REMARK 465     LYS A   105                                                      
REMARK 465     VAL A   106                                                      
REMARK 465     ASN A   181                                                      
REMARK 465     VAL A   182                                                      
REMARK 465     SER A   183                                                      
REMARK 465     ASP A   184                                                      
REMARK 465     GLU A   185                                                      
REMARK 465     ALA A   186                                                      
REMARK 465     GLN A   187                                                      
REMARK 465     GLU A   188                                                      
REMARK 465     ASP A   189                                                      
REMARK 465     GLU A   190                                                      
REMARK 465     GLU A   191                                                      
REMARK 465     HIS A   192                                                      
REMARK 465     TYR A   193                                                      
REMARK 465     LEU A   194                                                      
REMARK 465     MET A   195                                                      
REMARK 465     HIS A   196                                                      
REMARK 465     PRO A   197                                                      
REMARK 465     ALA A   198                                                      
REMARK 465     GLN A   199                                                      
REMARK 465     SER A   235                                                      
REMARK 465     GLY B    49                                                      
REMARK 465     SER B    50                                                      
REMARK 465     LEU B    51                                                      
REMARK 465     ARG B    52                                                      
REMARK 465     VAL B    53                                                      
REMARK 465     LYS B    54                                                      
REMARK 465     PHE B    55                                                      
REMARK 465     SER B    56                                                      
REMARK 465     ARG B    57                                                      
REMARK 465     SER B    58                                                      
REMARK 465     ALA B    59                                                      
REMARK 465     ASP B    60                                                      
REMARK 465     ALA B    61                                                      
REMARK 465     PRO B    62                                                      
REMARK 465     ARG B    79                                                      
REMARK 465     ARG B    80                                                      
REMARK 465     GLU B    81                                                      
REMARK 465     GLU B    82                                                      
REMARK 465     TYR B    83                                                      
REMARK 465     ASP B    84                                                      
REMARK 465     VAL B    85                                                      
REMARK 465     LEU B    86                                                      
REMARK 465     ASP B    87                                                      
REMARK 465     LYS B    88                                                      
REMARK 465     ARG B    89                                                      
REMARK 465     ARG B    90                                                      
REMARK 465     GLY B    91                                                      
REMARK 465     ARG B    92                                                      
REMARK 465     ASP B    93                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG A 109      136.69   -179.31                                   
REMARK 500    LEU A 115      -72.63    -63.52                                   
REMARK 500    ILE A 117      -72.75    -55.40                                   
REMARK 500    LEU A 129      -44.48     77.06                                   
REMARK 500    GLU A 130      107.13    -46.88                                   
REMARK 500    ASP A 143      -36.58    -39.74                                   
REMARK 500    PRO A 154       29.54    -72.56                                   
REMARK 500    PRO A 163     -143.59    -75.94                                   
REMARK 500    ASP A 204     -169.67    -64.55                                   
REMARK 500    ASP A 216      107.64   -160.53                                   
REMARK 500    THR A 222      102.13   -160.25                                   
REMARK 500    ALA A 225      -74.72    -54.43                                   
REMARK 500    GLU A 232       44.19    -98.42                                   
REMARK 500    ASN B  76       39.76     72.72                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 3IK5   RELATED DB: PDB                                   
DBREF  3IOZ A   95   235  UNP    Q5QGG3   Q5QGG3_SIVCZ    95    235             
DBREF  3IOZ B   51    93  UNP    P20963   CD3Z_HUMAN      51     93             
SEQADV 3IOZ GLY A   93  UNP  Q5QGG3              EXPRESSION TAG                 
SEQADV 3IOZ SER A   94  UNP  Q5QGG3              EXPRESSION TAG                 
SEQADV 3IOZ GLY B   49  UNP  P20963              EXPRESSION TAG                 
SEQADV 3IOZ SER B   50  UNP  P20963              EXPRESSION TAG                 
SEQRES   1 A  143  GLY SER ASP ASP LEU VAL GLY VAL SER VAL ARG PRO LYS          
SEQRES   2 A  143  VAL PRO LEU ARG THR MET SER TYR LYS LEU ALA ILE ASP          
SEQRES   3 A  143  MET SER HIS PHE ILE LYS GLU LYS GLY GLY LEU GLU GLY          
SEQRES   4 A  143  ILE TYR TYR SER ALA ARG ARG HIS ARG ILE LEU ASP ILE          
SEQRES   5 A  143  TYR LEU GLU LYS GLU GLU GLY ILE ILE PRO ASP TRP GLN          
SEQRES   6 A  143  ASP TYR THR SER GLY PRO GLY ILE ARG TYR PRO LYS THR          
SEQRES   7 A  143  PHE GLY TRP LEU TRP LYS LEU VAL PRO VAL ASN VAL SER          
SEQRES   8 A  143  ASP GLU ALA GLN GLU ASP GLU GLU HIS TYR LEU MET HIS          
SEQRES   9 A  143  PRO ALA GLN THR SER GLN TRP ASP ASP PRO TRP GLY GLU          
SEQRES  10 A  143  VAL LEU ALA TRP LYS PHE ASP PRO THR LEU ALA TYR THR          
SEQRES  11 A  143  TYR GLU ALA TYR VAL ARG TYR PRO GLU GLU PHE GLY SER          
SEQRES   1 B   45  GLY SER LEU ARG VAL LYS PHE SER ARG SER ALA ASP ALA          
SEQRES   2 B   45  PRO ALA TYR GLN GLN GLY GLN ASN GLN LEU TYR ASN GLU          
SEQRES   3 B   45  LEU ASN LEU GLY ARG ARG GLU GLU TYR ASP VAL LEU ASP          
SEQRES   4 B   45  LYS ARG ARG GLY ARG ASP                                      
HELIX    1   1 SER A  112  GLU A  125  1                                  14    
HELIX    2   2 SER A  135  GLY A  151  1                                  17    
HELIX    3   3 PRO A  217  TYR A  221  5                                   5    
HELIX    4   4 TYR A  223  TYR A  229  1                                   7    
HELIX    5   5 GLN B   65  GLU B   74  1                                  10    
SHEET    1   A 2 TRP A 175  VAL A 178  0                                        
SHEET    2   A 2 ALA A 212  PHE A 215 -1  O  LYS A 214   N  LYS A 176           
CISPEP   1 GLY A  162    PRO A  163          0        -0.02                     
CRYST1   51.638   51.638  189.449  90.00  90.00  90.00 P 43 21 2     8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.019366  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.019366  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.005278        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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