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Database: PDB
Entry: 3OQ4
LinkDB: 3OQ4
Original site: 3OQ4 
HEADER    CELL CYCLE                              02-SEP-10   3OQ4              
TITLE     CRYSTAL STRUCTURE OF MOTIF N OF SACCHAROMYCES CEREVISIAE DBF4         
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DBF4;                                                      
COMPND   3 CHAIN: A, B, C, D, E;                                                
COMPND   4 FRAGMENT: RESIDUES 120-250;                                          
COMPND   5 SYNONYM: PROTEIN DNA52;                                              
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE   3 ORGANISM_COMMON: BREWER'S YEAST,LAGER BEER YEAST,YEAST;              
SOURCE   4 ORGANISM_TAXID: 4932;                                                
SOURCE   5 GENE: D4205, DBF4, DNA52, YD9609.07C, YDR052C;                       
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)STARPRAREPLYSS;                   
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET15B                                    
KEYWDS    DDK, BRCT, RAD53, REPLICATION CHECKPOINT, FHA DOMAIN, REGULATORY      
KEYWDS   2 SUBUNIT OF DDK, CDC7, PHOSPHORYLATION, NUCLEAR, CELL CYCLE           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    L.A.MATTHEWS,D.R.JONES,A.A.PRASAD,B.P.DUNCKER,A.GUARNE                
REVDAT   1   07-SEP-11 3OQ4    0                                                
JRNL        AUTH   L.A.MATTHEWS,D.R.JONES,A.A.PRASAD,B.P.DUNCKER,A.GUARNE       
JRNL        TITL   CRYSTAL STRUCTURE OF SACCHAROMYCES CEREVISIAE DBF4-MOTIF N   
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   L.A.MATTHEWS,A.DUONG,A.A.PRASAD,B.P.DUNCKER,A.GUARNE         
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY X-RAY DIFFRACTION ANALYSIS   
REMARK   1  TITL 2 OF MOTIF N FROM SACCHAROMYCES CEREVISIAE DBF4                
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.F      V. F65   890 2009              
REMARK   1  REFN                   ESSN 1744-3091                               
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.40 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.6.4_486)                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.40                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 46.41                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 42245                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.205                           
REMARK   3   R VALUE            (WORKING SET) : 0.204                           
REMARK   3   FREE R VALUE                     : 0.229                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.080                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2146                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 46.4170 -  5.1665    0.99     4192   263  0.2032 0.2157        
REMARK   3     2  5.1665 -  4.1016    1.00     4091   205  0.1687 0.1931        
REMARK   3     3  4.1016 -  3.5834    1.00     4031   203  0.1839 0.1914        
REMARK   3     4  3.5834 -  3.2558    1.00     3997   213  0.2023 0.2298        
REMARK   3     5  3.2558 -  3.0225    1.00     4006   205  0.2087 0.2525        
REMARK   3     6  3.0225 -  2.8443    1.00     3958   216  0.2247 0.2320        
REMARK   3     7  2.8443 -  2.7019    1.00     3947   232  0.2312 0.2740        
REMARK   3     8  2.7019 -  2.5843    1.00     4015   191  0.2525 0.3165        
REMARK   3     9  2.5843 -  2.4848    1.00     3908   220  0.2478 0.2778        
REMARK   3    10  2.4848 -  2.3991    0.99     3954   198  0.2462 0.3272        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : 0.34                                          
REMARK   3   B_SOL              : 40.38                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.270            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 24.850           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 17.89630                                             
REMARK   3    B22 (A**2) : -1.54530                                             
REMARK   3    B33 (A**2) : -16.35100                                            
REMARK   3    B12 (A**2) : -0.00000                                             
REMARK   3    B13 (A**2) : -0.00000                                             
REMARK   3    B23 (A**2) : -0.00000                                             
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007           4429                                  
REMARK   3   ANGLE     :  0.946           5953                                  
REMARK   3   CHIRALITY :  0.065            683                                  
REMARK   3   PLANARITY :  0.003            739                                  
REMARK   3   DIHEDRAL  : 13.973           1714                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3OQ4 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 26-OCT-10.                  
REMARK 100 THE RCSB ID CODE IS RCSB061432.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 10-AUG-09                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.1                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : NSLS                               
REMARK 200  BEAMLINE                       : X29A                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0809                             
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : MIRRORS                            
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 42318                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.400                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY                : 6.200                              
REMARK 200  R MERGE                    (I) : 0.19200                            
REMARK 200  R SYM                      (I) : 0.20600                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 8.9200                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.40                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.44                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.73700                            
REMARK 200  R SYM FOR SHELL            (I) : 0.50200                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.600                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 3OQ0                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 63.76                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.39                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 22% MPD (V/V) AND 100 MM NA/K            
REMARK 280  PHOSPHATE BUFFER PH 7.1, VAPOR DIFFUSION, HANGING DROP,             
REMARK 280  TEMPERATURE 277K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       41.85700            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       49.85350            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       41.85700            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       49.85350            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3, 4, 5                                           
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 4                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 5                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A   117                                                      
REMARK 465     SER A   118                                                      
REMARK 465     LEU A   243                                                      
REMARK 465     TYR A   244                                                      
REMARK 465     GLY A   245                                                      
REMARK 465     PRO A   246                                                      
REMARK 465     THR A   247                                                      
REMARK 465     ASP A   248                                                      
REMARK 465     ARG A   249                                                      
REMARK 465     ASP A   250                                                      
REMARK 465     GLY B   117                                                      
REMARK 465     SER B   118                                                      
REMARK 465     HIS B   119                                                      
REMARK 465     MET B   120                                                      
REMARK 465     LYS B   121                                                      
REMARK 465     THR B   224                                                      
REMARK 465     LYS B   225                                                      
REMARK 465     SER B   226                                                      
REMARK 465     ALA B   227                                                      
REMARK 465     SER B   228                                                      
REMARK 465     LEU B   229                                                      
REMARK 465     ALA B   230                                                      
REMARK 465     ALA B   231                                                      
REMARK 465     PRO B   232                                                      
REMARK 465     THR B   233                                                      
REMARK 465     LEU B   234                                                      
REMARK 465     SER B   235                                                      
REMARK 465     ASN B   236                                                      
REMARK 465     LEU B   237                                                      
REMARK 465     LEU B   238                                                      
REMARK 465     HIS B   239                                                      
REMARK 465     ASN B   240                                                      
REMARK 465     GLU B   241                                                      
REMARK 465     LYS B   242                                                      
REMARK 465     LEU B   243                                                      
REMARK 465     TYR B   244                                                      
REMARK 465     GLY B   245                                                      
REMARK 465     PRO B   246                                                      
REMARK 465     THR B   247                                                      
REMARK 465     ASP B   248                                                      
REMARK 465     ARG B   249                                                      
REMARK 465     ASP B   250                                                      
REMARK 465     GLY C   117                                                      
REMARK 465     SER C   118                                                      
REMARK 465     HIS C   119                                                      
REMARK 465     MET C   120                                                      
REMARK 465     LYS C   121                                                      
REMARK 465     ARG C   122                                                      
REMARK 465     SER C   222                                                      
REMARK 465     LYS C   223                                                      
REMARK 465     THR C   224                                                      
REMARK 465     LYS C   225                                                      
REMARK 465     SER C   226                                                      
REMARK 465     ALA C   227                                                      
REMARK 465     SER C   228                                                      
REMARK 465     LEU C   229                                                      
REMARK 465     ALA C   230                                                      
REMARK 465     ALA C   231                                                      
REMARK 465     PRO C   232                                                      
REMARK 465     THR C   233                                                      
REMARK 465     LEU C   234                                                      
REMARK 465     SER C   235                                                      
REMARK 465     ASN C   236                                                      
REMARK 465     LEU C   237                                                      
REMARK 465     LEU C   238                                                      
REMARK 465     HIS C   239                                                      
REMARK 465     ASN C   240                                                      
REMARK 465     GLU C   241                                                      
REMARK 465     LYS C   242                                                      
REMARK 465     LEU C   243                                                      
REMARK 465     TYR C   244                                                      
REMARK 465     GLY C   245                                                      
REMARK 465     PRO C   246                                                      
REMARK 465     THR C   247                                                      
REMARK 465     ASP C   248                                                      
REMARK 465     ARG C   249                                                      
REMARK 465     ASP C   250                                                      
REMARK 465     GLY D   117                                                      
REMARK 465     SER D   118                                                      
REMARK 465     LEU D   218                                                      
REMARK 465     ASP D   219                                                      
REMARK 465     HIS D   220                                                      
REMARK 465     LEU D   221                                                      
REMARK 465     SER D   222                                                      
REMARK 465     LYS D   223                                                      
REMARK 465     THR D   224                                                      
REMARK 465     LYS D   225                                                      
REMARK 465     SER D   226                                                      
REMARK 465     ALA D   227                                                      
REMARK 465     SER D   228                                                      
REMARK 465     LEU D   229                                                      
REMARK 465     ALA D   230                                                      
REMARK 465     ALA D   231                                                      
REMARK 465     PRO D   232                                                      
REMARK 465     THR D   233                                                      
REMARK 465     LEU D   234                                                      
REMARK 465     SER D   235                                                      
REMARK 465     ASN D   236                                                      
REMARK 465     LEU D   237                                                      
REMARK 465     LEU D   238                                                      
REMARK 465     HIS D   239                                                      
REMARK 465     ASN D   240                                                      
REMARK 465     GLU D   241                                                      
REMARK 465     LYS D   242                                                      
REMARK 465     LEU D   243                                                      
REMARK 465     TYR D   244                                                      
REMARK 465     GLY D   245                                                      
REMARK 465     PRO D   246                                                      
REMARK 465     THR D   247                                                      
REMARK 465     ASP D   248                                                      
REMARK 465     ARG D   249                                                      
REMARK 465     ASP D   250                                                      
REMARK 465     GLY E   117                                                      
REMARK 465     SER E   118                                                      
REMARK 465     HIS E   119                                                      
REMARK 465     MET E   120                                                      
REMARK 465     ASP E   217                                                      
REMARK 465     LEU E   218                                                      
REMARK 465     ASP E   219                                                      
REMARK 465     HIS E   220                                                      
REMARK 465     LEU E   221                                                      
REMARK 465     SER E   222                                                      
REMARK 465     LYS E   223                                                      
REMARK 465     THR E   224                                                      
REMARK 465     LYS E   225                                                      
REMARK 465     SER E   226                                                      
REMARK 465     ALA E   227                                                      
REMARK 465     SER E   228                                                      
REMARK 465     LEU E   229                                                      
REMARK 465     ALA E   230                                                      
REMARK 465     ALA E   231                                                      
REMARK 465     PRO E   232                                                      
REMARK 465     THR E   233                                                      
REMARK 465     LEU E   234                                                      
REMARK 465     SER E   235                                                      
REMARK 465     ASN E   236                                                      
REMARK 465     LEU E   237                                                      
REMARK 465     LEU E   238                                                      
REMARK 465     HIS E   239                                                      
REMARK 465     ASN E   240                                                      
REMARK 465     GLU E   241                                                      
REMARK 465     LYS E   242                                                      
REMARK 465     LEU E   243                                                      
REMARK 465     TYR E   244                                                      
REMARK 465     GLY E   245                                                      
REMARK 465     PRO E   246                                                      
REMARK 465     THR E   247                                                      
REMARK 465     ASP E   248                                                      
REMARK 465     ARG E   249                                                      
REMARK 465     ASP E   250                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     HIS D 119    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ARG D 122    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG D 209    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ALA E 207    O                                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN D 197        9.88    -69.58                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 3OQ0   RELATED DB: PDB                                   
REMARK 900 (6XHIS)DBF4-N                                                        
DBREF  3OQ4 A  120   250  UNP    P32325   DBF4_YEAST     120    250             
DBREF  3OQ4 B  120   250  UNP    P32325   DBF4_YEAST     120    250             
DBREF  3OQ4 C  120   250  UNP    P32325   DBF4_YEAST     120    250             
DBREF  3OQ4 D  120   250  UNP    P32325   DBF4_YEAST     120    250             
DBREF  3OQ4 E  120   250  UNP    P32325   DBF4_YEAST     120    250             
SEQADV 3OQ4 GLY A  117  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 SER A  118  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 HIS A  119  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 GLY B  117  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 SER B  118  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 HIS B  119  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 GLY C  117  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 SER C  118  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 HIS C  119  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 GLY D  117  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 SER D  118  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 HIS D  119  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 GLY E  117  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 SER E  118  UNP  P32325              EXPRESSION TAG                 
SEQADV 3OQ4 HIS E  119  UNP  P32325              EXPRESSION TAG                 
SEQRES   1 A  134  GLY SER HIS MET LYS ARG ASP SER ARG ILE TYR PHE ASP          
SEQRES   2 A  134  ILE THR ASP ASP VAL GLU MET ASN THR TYR ASN LYS SER          
SEQRES   3 A  134  LYS MET ASP LYS ARG ARG ASP LEU LEU LYS ARG GLY PHE          
SEQRES   4 A  134  LEU THR LEU GLY ALA GLN ILE THR GLN PHE PHE ASP THR          
SEQRES   5 A  134  THR VAL THR ILE VAL ILE THR ARG ARG SER VAL GLU ASN          
SEQRES   6 A  134  ILE TYR LEU LEU LYS ASP THR ASP ILE LEU SER ARG ALA          
SEQRES   7 A  134  LYS LYS ASN TYR MET LYS VAL TRP SER TYR GLU LYS ALA          
SEQRES   8 A  134  ALA ARG PHE LEU LYS ASN LEU ASP VAL ASP LEU ASP HIS          
SEQRES   9 A  134  LEU SER LYS THR LYS SER ALA SER LEU ALA ALA PRO THR          
SEQRES  10 A  134  LEU SER ASN LEU LEU HIS ASN GLU LYS LEU TYR GLY PRO          
SEQRES  11 A  134  THR ASP ARG ASP                                              
SEQRES   1 B  134  GLY SER HIS MET LYS ARG ASP SER ARG ILE TYR PHE ASP          
SEQRES   2 B  134  ILE THR ASP ASP VAL GLU MET ASN THR TYR ASN LYS SER          
SEQRES   3 B  134  LYS MET ASP LYS ARG ARG ASP LEU LEU LYS ARG GLY PHE          
SEQRES   4 B  134  LEU THR LEU GLY ALA GLN ILE THR GLN PHE PHE ASP THR          
SEQRES   5 B  134  THR VAL THR ILE VAL ILE THR ARG ARG SER VAL GLU ASN          
SEQRES   6 B  134  ILE TYR LEU LEU LYS ASP THR ASP ILE LEU SER ARG ALA          
SEQRES   7 B  134  LYS LYS ASN TYR MET LYS VAL TRP SER TYR GLU LYS ALA          
SEQRES   8 B  134  ALA ARG PHE LEU LYS ASN LEU ASP VAL ASP LEU ASP HIS          
SEQRES   9 B  134  LEU SER LYS THR LYS SER ALA SER LEU ALA ALA PRO THR          
SEQRES  10 B  134  LEU SER ASN LEU LEU HIS ASN GLU LYS LEU TYR GLY PRO          
SEQRES  11 B  134  THR ASP ARG ASP                                              
SEQRES   1 C  134  GLY SER HIS MET LYS ARG ASP SER ARG ILE TYR PHE ASP          
SEQRES   2 C  134  ILE THR ASP ASP VAL GLU MET ASN THR TYR ASN LYS SER          
SEQRES   3 C  134  LYS MET ASP LYS ARG ARG ASP LEU LEU LYS ARG GLY PHE          
SEQRES   4 C  134  LEU THR LEU GLY ALA GLN ILE THR GLN PHE PHE ASP THR          
SEQRES   5 C  134  THR VAL THR ILE VAL ILE THR ARG ARG SER VAL GLU ASN          
SEQRES   6 C  134  ILE TYR LEU LEU LYS ASP THR ASP ILE LEU SER ARG ALA          
SEQRES   7 C  134  LYS LYS ASN TYR MET LYS VAL TRP SER TYR GLU LYS ALA          
SEQRES   8 C  134  ALA ARG PHE LEU LYS ASN LEU ASP VAL ASP LEU ASP HIS          
SEQRES   9 C  134  LEU SER LYS THR LYS SER ALA SER LEU ALA ALA PRO THR          
SEQRES  10 C  134  LEU SER ASN LEU LEU HIS ASN GLU LYS LEU TYR GLY PRO          
SEQRES  11 C  134  THR ASP ARG ASP                                              
SEQRES   1 D  134  GLY SER HIS MET LYS ARG ASP SER ARG ILE TYR PHE ASP          
SEQRES   2 D  134  ILE THR ASP ASP VAL GLU MET ASN THR TYR ASN LYS SER          
SEQRES   3 D  134  LYS MET ASP LYS ARG ARG ASP LEU LEU LYS ARG GLY PHE          
SEQRES   4 D  134  LEU THR LEU GLY ALA GLN ILE THR GLN PHE PHE ASP THR          
SEQRES   5 D  134  THR VAL THR ILE VAL ILE THR ARG ARG SER VAL GLU ASN          
SEQRES   6 D  134  ILE TYR LEU LEU LYS ASP THR ASP ILE LEU SER ARG ALA          
SEQRES   7 D  134  LYS LYS ASN TYR MET LYS VAL TRP SER TYR GLU LYS ALA          
SEQRES   8 D  134  ALA ARG PHE LEU LYS ASN LEU ASP VAL ASP LEU ASP HIS          
SEQRES   9 D  134  LEU SER LYS THR LYS SER ALA SER LEU ALA ALA PRO THR          
SEQRES  10 D  134  LEU SER ASN LEU LEU HIS ASN GLU LYS LEU TYR GLY PRO          
SEQRES  11 D  134  THR ASP ARG ASP                                              
SEQRES   1 E  134  GLY SER HIS MET LYS ARG ASP SER ARG ILE TYR PHE ASP          
SEQRES   2 E  134  ILE THR ASP ASP VAL GLU MET ASN THR TYR ASN LYS SER          
SEQRES   3 E  134  LYS MET ASP LYS ARG ARG ASP LEU LEU LYS ARG GLY PHE          
SEQRES   4 E  134  LEU THR LEU GLY ALA GLN ILE THR GLN PHE PHE ASP THR          
SEQRES   5 E  134  THR VAL THR ILE VAL ILE THR ARG ARG SER VAL GLU ASN          
SEQRES   6 E  134  ILE TYR LEU LEU LYS ASP THR ASP ILE LEU SER ARG ALA          
SEQRES   7 E  134  LYS LYS ASN TYR MET LYS VAL TRP SER TYR GLU LYS ALA          
SEQRES   8 E  134  ALA ARG PHE LEU LYS ASN LEU ASP VAL ASP LEU ASP HIS          
SEQRES   9 E  134  LEU SER LYS THR LYS SER ALA SER LEU ALA ALA PRO THR          
SEQRES  10 E  134  LEU SER ASN LEU LEU HIS ASN GLU LYS LEU TYR GLY PRO          
SEQRES  11 E  134  THR ASP ARG ASP                                              
FORMUL   6  HOH   *230(H2 O)                                                    
HELIX    1   1 ASN A  137  LEU A  158  1                                  22    
HELIX    2   2 SER A  178  LEU A  185  5                                   8    
HELIX    3   3 ASP A  189  ASN A  197  1                                   9    
HELIX    4   4 TYR A  204  LEU A  214  1                                  11    
HELIX    5   5 ASP A  217  SER A  222  1                                   6    
HELIX    6   6 PRO A  232  LYS A  242  1                                  11    
HELIX    7   7 ASN B  137  THR B  157  1                                  21    
HELIX    8   8 SER B  178  LEU B  185  5                                   8    
HELIX    9   9 ASP B  189  ASN B  197  1                                   9    
HELIX   10  10 TYR B  204  LEU B  214  1                                  11    
HELIX   11  11 ASP B  217  LYS B  223  1                                   7    
HELIX   12  12 ASN C  137  GLY C  159  1                                  23    
HELIX   13  13 SER C  178  LEU C  185  5                                   8    
HELIX   14  14 ASP C  189  ASN C  197  1                                   9    
HELIX   15  15 TYR C  204  LEU C  214  1                                  11    
HELIX   16  16 ASN D  137  LEU D  158  1                                  22    
HELIX   17  17 SER D  178  LEU D  185  5                                   8    
HELIX   18  18 ASP D  189  ASN D  197  1                                   9    
HELIX   19  19 TYR D  204  LEU D  214  1                                  11    
HELIX   20  20 ASN E  137  LEU E  158  1                                  22    
HELIX   21  21 ASN E  181  LEU E  185  5                                   5    
HELIX   22  22 ASP E  189  ASN E  197  1                                   9    
HELIX   23  23 GLU E  205  ASP E  215  1                                  11    
SHEET    1   A 5 LYS A 200  SER A 203  0                                        
SHEET    2   A 5 ILE A 172  THR A 175  1  N  THR A 175   O  TRP A 202           
SHEET    3   A 5 ARG A 125  PHE A 128  1  N  TYR A 127   O  ILE A 172           
SHEET    4   A 5 GLN A 161  THR A 163  1  O  GLN A 161   N  ILE A 126           
SHEET    5   A 5 VAL B 134  GLU B 135 -1  O  GLU B 135   N  ILE A 162           
SHEET    1   B 5 LYS B 200  SER B 203  0                                        
SHEET    2   B 5 ILE B 172  THR B 175  1  N  VAL B 173   O  LYS B 200           
SHEET    3   B 5 ARG B 125  PHE B 128  1  N  TYR B 127   O  ILE B 174           
SHEET    4   B 5 GLN B 161  THR B 163  1  O  GLN B 161   N  ILE B 126           
SHEET    5   B 5 VAL C 134  GLU C 135 -1  O  GLU C 135   N  ILE B 162           
SHEET    1   C 5 LYS C 200  SER C 203  0                                        
SHEET    2   C 5 ILE C 172  THR C 175  1  N  VAL C 173   O  LYS C 200           
SHEET    3   C 5 ARG C 125  PHE C 128  1  N  TYR C 127   O  ILE C 172           
SHEET    4   C 5 GLN C 161  THR C 163  1  O  GLN C 161   N  ILE C 126           
SHEET    5   C 5 VAL D 134  GLU D 135 -1  O  GLU D 135   N  ILE C 162           
SHEET    1   D 5 LYS D 200  SER D 203  0                                        
SHEET    2   D 5 ILE D 172  THR D 175  1  N  THR D 175   O  TRP D 202           
SHEET    3   D 5 ARG D 125  PHE D 128  1  N  TYR D 127   O  ILE D 174           
SHEET    4   D 5 GLN D 161  THR D 163  1  O  GLN D 161   N  ILE D 126           
SHEET    5   D 5 VAL E 134  GLU E 135 -1  O  GLU E 135   N  ILE D 162           
SHEET    1   E 4 GLN E 161  ILE E 162  0                                        
SHEET    2   E 4 ARG E 125  PHE E 128  1  N  ILE E 126   O  GLN E 161           
SHEET    3   E 4 ILE E 172  THR E 175  1  O  ILE E 174   N  TYR E 127           
SHEET    4   E 4 LYS E 200  SER E 203  1  O  LYS E 200   N  VAL E 173           
CRYST1   83.714   99.707  127.045  90.00  90.00  90.00 P 21 21 2    20          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.011945  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.010029  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.007871        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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