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Database: PDB
Entry: 3VPJ
LinkDB: 3VPJ
Original site: 3VPJ 
HEADER    HYDROLASE/HYDROLASE INHIBITOR           04-MAR-12   3VPJ              
TITLE     CRYSTAL STRUCTURE OF TYPE VI EFFECTOR TSE1 FROM PSEUDOMONAS AERUGINOSA
TITLE    2 IN COMPLEX WITH IMMUNE PROTEIN TSI1                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TYPE VI SECRETION EXPORTED 1;                              
COMPND   3 CHAIN: A, B, C, D;                                                   
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: TSE1-SPECIFIC IMMUNITY PROTEIN;                            
COMPND   7 CHAIN: E, F, G, H;                                                   
COMPND   8 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA;                         
SOURCE   3 ORGANISM_TAXID: 208964;                                              
SOURCE   4 STRAIN: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;                
SOURCE   5 GENE: PA1844;                                                        
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28A;                                   
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA;                         
SOURCE  13 ORGANISM_TAXID: 208964;                                              
SOURCE  14 GENE: PA1845;                                                        
SOURCE  15 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  16 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  17 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  18 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  19 EXPRESSION_SYSTEM_PLASMID: PET28A                                    
KEYWDS    HYDROLASE-HYDROLASE INHIBITOR COMPLEX                                 
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.DING,W.WANG,D.C.WANG                                                
REVDAT   2   07-AUG-13 3VPJ    1       JRNL                                     
REVDAT   1   27-JUN-12 3VPJ    0                                                
JRNL        AUTH   J.DING,W.WANG,H.FENG,Y.ZHANG,D.C.WANG                        
JRNL        TITL   STRUCTURAL INSIGHTS INTO THE PSEUDOMONAS AERUGINOSA TYPE VI  
JRNL        TITL 2 VIRULENCE EFFECTOR TSE1 BACTERIOLYSIS AND SELF-PROTECTION    
JRNL        TITL 3 MECHANISMS                                                   
JRNL        REF    J.BIOL.CHEM.                  V. 287 26911 2012              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   22700987                                                     
JRNL        DOI    10.1074/JBC.M112.368043                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.7_650)                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 47.97                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 55504                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.209                           
REMARK   3   R VALUE            (WORKING SET) : 0.207                           
REMARK   3   FREE R VALUE                     : 0.257                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.080                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2818                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 47.9787 -  6.7800    0.99     2885   146  0.1764 0.1855        
REMARK   3     2  6.7800 -  5.3838    1.00     2746   140  0.1978 0.2440        
REMARK   3     3  5.3838 -  4.7039    1.00     2742   143  0.1520 0.1970        
REMARK   3     4  4.7039 -  4.2742    1.00     2685   142  0.1470 0.1811        
REMARK   3     5  4.2742 -  3.9680    1.00     2691   146  0.1712 0.2259        
REMARK   3     6  3.9680 -  3.7341    1.00     2634   152  0.1932 0.2472        
REMARK   3     7  3.7341 -  3.5472    1.00     2693   144  0.2110 0.2747        
REMARK   3     8  3.5472 -  3.3928    1.00     2657   138  0.2208 0.2928        
REMARK   3     9  3.3928 -  3.2622    0.99     2632   148  0.2434 0.2845        
REMARK   3    10  3.2622 -  3.1497    1.00     2611   143  0.2400 0.2989        
REMARK   3    11  3.1497 -  3.0512    0.99     2628   141  0.2507 0.2683        
REMARK   3    12  3.0512 -  2.9640    0.99     2625   158  0.2483 0.3674        
REMARK   3    13  2.9640 -  2.8860    0.99     2582   149  0.2579 0.2881        
REMARK   3    14  2.8860 -  2.8156    0.98     2597   126  0.2583 0.3408        
REMARK   3    15  2.8156 -  2.7516    0.99     2605   125  0.2637 0.3257        
REMARK   3    16  2.7516 -  2.6930    0.98     2547   137  0.2813 0.4029        
REMARK   3    17  2.6930 -  2.6392    0.97     2564   145  0.2819 0.3231        
REMARK   3    18  2.6392 -  2.5894    0.97     2532   133  0.2828 0.4039        
REMARK   3    19  2.5894 -  2.5431    0.96     2486   131  0.2826 0.3292        
REMARK   3    20  2.5431 -  2.5000    0.95     2544   131  0.2736 0.3198        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : 0.32                                          
REMARK   3   B_SOL              : 36.52                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.370            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.290           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 56.90                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 52.87                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 2.84630                                              
REMARK   3    B22 (A**2) : 2.84630                                              
REMARK   3    B33 (A**2) : -5.69270                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : -0.00000                                             
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.011           9388                                  
REMARK   3   ANGLE     :  1.059          12295                                  
REMARK   3   CHIRALITY :  0.076           1292                                  
REMARK   3   PLANARITY :  0.004           1646                                  
REMARK   3   DIHEDRAL  : 14.369           3308                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3VPJ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 08-MAR-12.                  
REMARK 100 THE RCSB ID CODE IS RCSB095339.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-NOV-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 95                                 
REMARK 200  PH                             : 5.4                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : PHOTON FACTORY                     
REMARK 200  BEAMLINE                       : BL-17A                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : NULL                               
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM                             
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 56166                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 63.610                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY                : 9.200                              
REMARK 200  R MERGE                    (I) : 0.06700                            
REMARK 200  R SYM                      (I) : 0.06700                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 21.6000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.64                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.4                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 8.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.38700                            
REMARK 200  R SYM FOR SHELL            (I) : 0.38700                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 5.600                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 50.92                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.51                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 20% PEG 4000, 20% ISOPROPANOL, 0.1M      
REMARK 280  SODIUM CITRATE, PH 5.4, VAPOR DIFFUSION, HANGING DROP,              
REMARK 280  TEMPERATURE 293K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 32 2 1                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   Y,X,-Z                                                  
REMARK 290       5555   X-Y,-Y,-Z+1/3                                           
REMARK 290       6555   -X,-X+Y,-Z+2/3                                          
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      194.78067            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       97.39033            
REMARK 290   SMTRY1   4 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   5  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       97.39033            
REMARK 290   SMTRY1   6 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000      194.78067            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3, 4                                              
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2030 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13190 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -4.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, E                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1930 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13120 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -2.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, F                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2050 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13310 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -2.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, G                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 4                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2060 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13080 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -3.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D, H                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   -19                                                      
REMARK 465     GLY A   -18                                                      
REMARK 465     SER A   -17                                                      
REMARK 465     SER A   -16                                                      
REMARK 465     HIS A   -15                                                      
REMARK 465     HIS A   -14                                                      
REMARK 465     HIS A   -13                                                      
REMARK 465     HIS A   -12                                                      
REMARK 465     HIS A   -11                                                      
REMARK 465     HIS A   -10                                                      
REMARK 465     SER A    -9                                                      
REMARK 465     SER A    -8                                                      
REMARK 465     GLY A    -7                                                      
REMARK 465     LEU A    -6                                                      
REMARK 465     VAL A    -5                                                      
REMARK 465     PRO A    -4                                                      
REMARK 465     ARG A    -3                                                      
REMARK 465     GLY A    -2                                                      
REMARK 465     SER A    -1                                                      
REMARK 465     HIS A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     ASP A     2                                                      
REMARK 465     SER A   149                                                      
REMARK 465     LEU A   150                                                      
REMARK 465     PRO A   151                                                      
REMARK 465     ARG A   152                                                      
REMARK 465     ALA A   153                                                      
REMARK 465     SER A   154                                                      
REMARK 465     MET B   -19                                                      
REMARK 465     GLY B   -18                                                      
REMARK 465     SER B   -17                                                      
REMARK 465     SER B   -16                                                      
REMARK 465     HIS B   -15                                                      
REMARK 465     HIS B   -14                                                      
REMARK 465     HIS B   -13                                                      
REMARK 465     HIS B   -12                                                      
REMARK 465     HIS B   -11                                                      
REMARK 465     HIS B   -10                                                      
REMARK 465     SER B    -9                                                      
REMARK 465     SER B    -8                                                      
REMARK 465     GLY B    -7                                                      
REMARK 465     LEU B    -6                                                      
REMARK 465     VAL B    -5                                                      
REMARK 465     PRO B    -4                                                      
REMARK 465     ARG B    -3                                                      
REMARK 465     GLY B    -2                                                      
REMARK 465     SER B    -1                                                      
REMARK 465     HIS B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     ASP B     2                                                      
REMARK 465     SER B     3                                                      
REMARK 465     SER B   149                                                      
REMARK 465     LEU B   150                                                      
REMARK 465     PRO B   151                                                      
REMARK 465     ARG B   152                                                      
REMARK 465     ALA B   153                                                      
REMARK 465     SER B   154                                                      
REMARK 465     MET E   -19                                                      
REMARK 465     GLY E   -18                                                      
REMARK 465     SER E   -17                                                      
REMARK 465     SER E   -16                                                      
REMARK 465     HIS E   -15                                                      
REMARK 465     HIS E   -14                                                      
REMARK 465     HIS E   -13                                                      
REMARK 465     HIS E   -12                                                      
REMARK 465     HIS E   -11                                                      
REMARK 465     HIS E   -10                                                      
REMARK 465     SER E    -9                                                      
REMARK 465     SER E    -8                                                      
REMARK 465     GLY E    -7                                                      
REMARK 465     LEU E    -6                                                      
REMARK 465     VAL E    -5                                                      
REMARK 465     PRO E    -4                                                      
REMARK 465     ARG E    -3                                                      
REMARK 465     GLY E    -2                                                      
REMARK 465     SER E    -1                                                      
REMARK 465     HIS E     0                                                      
REMARK 465     MET E     1                                                      
REMARK 465     LYS E     2                                                      
REMARK 465     LEU E     3                                                      
REMARK 465     LEU E     4                                                      
REMARK 465     ALA E     5                                                      
REMARK 465     GLY E     6                                                      
REMARK 465     SER E     7                                                      
REMARK 465     PHE E     8                                                      
REMARK 465     ALA E     9                                                      
REMARK 465     ALA E    10                                                      
REMARK 465     LEU E    11                                                      
REMARK 465     PHE E    12                                                      
REMARK 465     LEU E    13                                                      
REMARK 465     SER E    14                                                      
REMARK 465     LEU E    15                                                      
REMARK 465     SER E    16                                                      
REMARK 465     ALA E    17                                                      
REMARK 465     GLN E    18                                                      
REMARK 465     ALA E    19                                                      
REMARK 465     ALA E    20                                                      
REMARK 465     THR E   169                                                      
REMARK 465     ALA E   170                                                      
REMARK 465     LYS E   171                                                      
REMARK 465     LYS E   172                                                      
REMARK 465     MET F   -19                                                      
REMARK 465     GLY F   -18                                                      
REMARK 465     SER F   -17                                                      
REMARK 465     SER F   -16                                                      
REMARK 465     HIS F   -15                                                      
REMARK 465     HIS F   -14                                                      
REMARK 465     HIS F   -13                                                      
REMARK 465     HIS F   -12                                                      
REMARK 465     HIS F   -11                                                      
REMARK 465     HIS F   -10                                                      
REMARK 465     SER F    -9                                                      
REMARK 465     SER F    -8                                                      
REMARK 465     GLY F    -7                                                      
REMARK 465     LEU F    -6                                                      
REMARK 465     VAL F    -5                                                      
REMARK 465     PRO F    -4                                                      
REMARK 465     ARG F    -3                                                      
REMARK 465     GLY F    -2                                                      
REMARK 465     SER F    -1                                                      
REMARK 465     HIS F     0                                                      
REMARK 465     MET F     1                                                      
REMARK 465     LYS F     2                                                      
REMARK 465     LEU F     3                                                      
REMARK 465     LEU F     4                                                      
REMARK 465     ALA F     5                                                      
REMARK 465     GLY F     6                                                      
REMARK 465     SER F     7                                                      
REMARK 465     PHE F     8                                                      
REMARK 465     ALA F     9                                                      
REMARK 465     ALA F    10                                                      
REMARK 465     LEU F    11                                                      
REMARK 465     PHE F    12                                                      
REMARK 465     LEU F    13                                                      
REMARK 465     SER F    14                                                      
REMARK 465     LEU F    15                                                      
REMARK 465     SER F    16                                                      
REMARK 465     ALA F    17                                                      
REMARK 465     GLN F    18                                                      
REMARK 465     ALA F    19                                                      
REMARK 465     ALA F    20                                                      
REMARK 465     GLN F   168                                                      
REMARK 465     THR F   169                                                      
REMARK 465     ALA F   170                                                      
REMARK 465     LYS F   171                                                      
REMARK 465     LYS F   172                                                      
REMARK 465     MET C   -19                                                      
REMARK 465     GLY C   -18                                                      
REMARK 465     SER C   -17                                                      
REMARK 465     SER C   -16                                                      
REMARK 465     HIS C   -15                                                      
REMARK 465     HIS C   -14                                                      
REMARK 465     HIS C   -13                                                      
REMARK 465     HIS C   -12                                                      
REMARK 465     HIS C   -11                                                      
REMARK 465     HIS C   -10                                                      
REMARK 465     SER C    -9                                                      
REMARK 465     SER C    -8                                                      
REMARK 465     GLY C    -7                                                      
REMARK 465     LEU C    -6                                                      
REMARK 465     VAL C    -5                                                      
REMARK 465     PRO C    -4                                                      
REMARK 465     ARG C    -3                                                      
REMARK 465     GLY C    -2                                                      
REMARK 465     SER C    -1                                                      
REMARK 465     HIS C     0                                                      
REMARK 465     MET C     1                                                      
REMARK 465     ASP C     2                                                      
REMARK 465     SER C     3                                                      
REMARK 465     SER C   149                                                      
REMARK 465     LEU C   150                                                      
REMARK 465     PRO C   151                                                      
REMARK 465     ARG C   152                                                      
REMARK 465     ALA C   153                                                      
REMARK 465     SER C   154                                                      
REMARK 465     MET D   -19                                                      
REMARK 465     GLY D   -18                                                      
REMARK 465     SER D   -17                                                      
REMARK 465     SER D   -16                                                      
REMARK 465     HIS D   -15                                                      
REMARK 465     HIS D   -14                                                      
REMARK 465     HIS D   -13                                                      
REMARK 465     HIS D   -12                                                      
REMARK 465     HIS D   -11                                                      
REMARK 465     HIS D   -10                                                      
REMARK 465     SER D    -9                                                      
REMARK 465     SER D    -8                                                      
REMARK 465     GLY D    -7                                                      
REMARK 465     LEU D    -6                                                      
REMARK 465     VAL D    -5                                                      
REMARK 465     PRO D    -4                                                      
REMARK 465     ARG D    -3                                                      
REMARK 465     GLY D    -2                                                      
REMARK 465     SER D    -1                                                      
REMARK 465     HIS D     0                                                      
REMARK 465     MET D     1                                                      
REMARK 465     ASP D     2                                                      
REMARK 465     SER D   149                                                      
REMARK 465     LEU D   150                                                      
REMARK 465     PRO D   151                                                      
REMARK 465     ARG D   152                                                      
REMARK 465     ALA D   153                                                      
REMARK 465     SER D   154                                                      
REMARK 465     MET G   -19                                                      
REMARK 465     GLY G   -18                                                      
REMARK 465     SER G   -17                                                      
REMARK 465     SER G   -16                                                      
REMARK 465     HIS G   -15                                                      
REMARK 465     HIS G   -14                                                      
REMARK 465     HIS G   -13                                                      
REMARK 465     HIS G   -12                                                      
REMARK 465     HIS G   -11                                                      
REMARK 465     HIS G   -10                                                      
REMARK 465     SER G    -9                                                      
REMARK 465     SER G    -8                                                      
REMARK 465     GLY G    -7                                                      
REMARK 465     LEU G    -6                                                      
REMARK 465     VAL G    -5                                                      
REMARK 465     PRO G    -4                                                      
REMARK 465     ARG G    -3                                                      
REMARK 465     GLY G    -2                                                      
REMARK 465     SER G    -1                                                      
REMARK 465     HIS G     0                                                      
REMARK 465     MET G     1                                                      
REMARK 465     LYS G     2                                                      
REMARK 465     LEU G     3                                                      
REMARK 465     LEU G     4                                                      
REMARK 465     ALA G     5                                                      
REMARK 465     GLY G     6                                                      
REMARK 465     SER G     7                                                      
REMARK 465     PHE G     8                                                      
REMARK 465     ALA G     9                                                      
REMARK 465     ALA G    10                                                      
REMARK 465     LEU G    11                                                      
REMARK 465     PHE G    12                                                      
REMARK 465     LEU G    13                                                      
REMARK 465     SER G    14                                                      
REMARK 465     LEU G    15                                                      
REMARK 465     SER G    16                                                      
REMARK 465     ALA G    17                                                      
REMARK 465     GLN G    18                                                      
REMARK 465     ALA G    19                                                      
REMARK 465     ALA G    20                                                      
REMARK 465     THR G   169                                                      
REMARK 465     ALA G   170                                                      
REMARK 465     LYS G   171                                                      
REMARK 465     LYS G   172                                                      
REMARK 465     MET H   -19                                                      
REMARK 465     GLY H   -18                                                      
REMARK 465     SER H   -17                                                      
REMARK 465     SER H   -16                                                      
REMARK 465     HIS H   -15                                                      
REMARK 465     HIS H   -14                                                      
REMARK 465     HIS H   -13                                                      
REMARK 465     HIS H   -12                                                      
REMARK 465     HIS H   -11                                                      
REMARK 465     HIS H   -10                                                      
REMARK 465     SER H    -9                                                      
REMARK 465     SER H    -8                                                      
REMARK 465     GLY H    -7                                                      
REMARK 465     LEU H    -6                                                      
REMARK 465     VAL H    -5                                                      
REMARK 465     PRO H    -4                                                      
REMARK 465     ARG H    -3                                                      
REMARK 465     GLY H    -2                                                      
REMARK 465     SER H    -1                                                      
REMARK 465     HIS H     0                                                      
REMARK 465     MET H     1                                                      
REMARK 465     LYS H     2                                                      
REMARK 465     LEU H     3                                                      
REMARK 465     LEU H     4                                                      
REMARK 465     ALA H     5                                                      
REMARK 465     GLY H     6                                                      
REMARK 465     SER H     7                                                      
REMARK 465     PHE H     8                                                      
REMARK 465     ALA H     9                                                      
REMARK 465     ALA H    10                                                      
REMARK 465     LEU H    11                                                      
REMARK 465     PHE H    12                                                      
REMARK 465     LEU H    13                                                      
REMARK 465     SER H    14                                                      
REMARK 465     LEU H    15                                                      
REMARK 465     SER H    16                                                      
REMARK 465     ALA H    17                                                      
REMARK 465     GLN H    18                                                      
REMARK 465     ALA H    19                                                      
REMARK 465     ALA H    20                                                      
REMARK 465     ASP H    21                                                      
REMARK 465     THR H   169                                                      
REMARK 465     ALA H   170                                                      
REMARK 465     LYS H   171                                                      
REMARK 465     LYS H   172                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ASP G  21    CG   OD1  OD2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    THR A  88      -63.08   -128.34                                   
REMARK 500    GLN A 103       19.72     57.00                                   
REMARK 500    LYS A 104      -59.46   -132.38                                   
REMARK 500    GLN B  59      -70.26    -79.14                                   
REMARK 500    THR B  88      -60.70    -95.23                                   
REMARK 500    LYS B 104      -61.93   -132.58                                   
REMARK 500    VAL B 129      -61.78   -101.67                                   
REMARK 500    ASN E  37       19.86     58.23                                   
REMARK 500    LYS E 146       73.31     56.72                                   
REMARK 500    ASP F  43     -169.76   -126.63                                   
REMARK 500    HIS F  45      -60.58   -105.18                                   
REMARK 500    THR C  88      -61.75   -127.46                                   
REMARK 500    GLN C 103       -4.10     69.42                                   
REMARK 500    GLN D  59      -72.48    -81.01                                   
REMARK 500    THR D  88      -62.43   -101.11                                   
REMARK 500    LYS D 104      -54.72   -128.37                                   
REMARK 500    ASN G  37       -0.67     70.72                                   
REMARK 500    ASN H  37       -8.42     68.95                                   
REMARK 500    CYS H 121       -0.79     68.53                                   
REMARK 500    GLN H 145      -75.42   -117.19                                   
REMARK 500    ALA H 156      -70.45    -76.35                                   
REMARK 500    CYS H 167      -78.31   -110.79                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 3VPI   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF TYPE VI EFFECTOR TSE1 FROM PSEUDOMONAS          
REMARK 900 AERUGINOSA                                                           
DBREF  3VPJ A    1   154  UNP    Q9I2Q1   Q9I2Q1_PSEAE     1    154             
DBREF  3VPJ B    1   154  UNP    Q9I2Q1   Q9I2Q1_PSEAE     1    154             
DBREF  3VPJ E    1   172  UNP    Q9I2Q0   Q9I2Q0_PSEAE     1    172             
DBREF  3VPJ F    1   172  UNP    Q9I2Q0   Q9I2Q0_PSEAE     1    172             
DBREF  3VPJ C    1   154  UNP    Q9I2Q1   Q9I2Q1_PSEAE     1    154             
DBREF  3VPJ D    1   154  UNP    Q9I2Q1   Q9I2Q1_PSEAE     1    154             
DBREF  3VPJ G    1   172  UNP    Q9I2Q0   Q9I2Q0_PSEAE     1    172             
DBREF  3VPJ H    1   172  UNP    Q9I2Q0   Q9I2Q0_PSEAE     1    172             
SEQADV 3VPJ MET A  -19  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY A  -18  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER A  -17  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER A  -16  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A  -15  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A  -14  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A  -13  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A  -12  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A  -11  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A  -10  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER A   -9  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER A   -8  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY A   -7  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ LEU A   -6  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ VAL A   -5  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ PRO A   -4  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ ARG A   -3  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY A   -2  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER A   -1  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS A    0  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ MET B  -19  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY B  -18  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER B  -17  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER B  -16  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B  -15  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B  -14  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B  -13  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B  -12  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B  -11  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B  -10  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER B   -9  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER B   -8  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY B   -7  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ LEU B   -6  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ VAL B   -5  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ PRO B   -4  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ ARG B   -3  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY B   -2  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER B   -1  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS B    0  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ MET E  -19  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY E  -18  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER E  -17  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER E  -16  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E  -15  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E  -14  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E  -13  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E  -12  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E  -11  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E  -10  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER E   -9  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER E   -8  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY E   -7  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ LEU E   -6  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ VAL E   -5  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ PRO E   -4  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ ARG E   -3  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY E   -2  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER E   -1  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS E    0  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ MET F  -19  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY F  -18  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER F  -17  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER F  -16  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F  -15  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F  -14  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F  -13  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F  -12  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F  -11  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F  -10  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER F   -9  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER F   -8  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY F   -7  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ LEU F   -6  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ VAL F   -5  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ PRO F   -4  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ ARG F   -3  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY F   -2  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER F   -1  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS F    0  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ MET C  -19  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY C  -18  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER C  -17  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER C  -16  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C  -15  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C  -14  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C  -13  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C  -12  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C  -11  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C  -10  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER C   -9  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER C   -8  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY C   -7  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ LEU C   -6  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ VAL C   -5  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ PRO C   -4  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ ARG C   -3  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY C   -2  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER C   -1  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS C    0  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ MET D  -19  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY D  -18  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER D  -17  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER D  -16  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D  -15  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D  -14  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D  -13  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D  -12  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D  -11  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D  -10  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER D   -9  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER D   -8  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY D   -7  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ LEU D   -6  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ VAL D   -5  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ PRO D   -4  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ ARG D   -3  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ GLY D   -2  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ SER D   -1  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ HIS D    0  UNP  Q9I2Q1              EXPRESSION TAG                 
SEQADV 3VPJ MET G  -19  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY G  -18  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER G  -17  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER G  -16  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G  -15  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G  -14  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G  -13  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G  -12  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G  -11  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G  -10  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER G   -9  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER G   -8  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY G   -7  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ LEU G   -6  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ VAL G   -5  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ PRO G   -4  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ ARG G   -3  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY G   -2  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER G   -1  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS G    0  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ MET H  -19  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY H  -18  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER H  -17  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER H  -16  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H  -15  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H  -14  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H  -13  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H  -12  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H  -11  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H  -10  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER H   -9  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER H   -8  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY H   -7  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ LEU H   -6  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ VAL H   -5  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ PRO H   -4  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ ARG H   -3  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ GLY H   -2  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ SER H   -1  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQADV 3VPJ HIS H    0  UNP  Q9I2Q0              EXPRESSION TAG                 
SEQRES   1 A  174  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 A  174  LEU VAL PRO ARG GLY SER HIS MET ASP SER LEU ASP GLN          
SEQRES   3 A  174  CYS ILE VAL ASN ALA CYS LYS ASN SER TRP ASP LYS SER          
SEQRES   4 A  174  TYR LEU ALA GLY THR PRO ASN LYS ASP ASN CYS SER GLY          
SEQRES   5 A  174  PHE VAL GLN SER VAL ALA ALA GLU LEU GLY VAL PRO MET          
SEQRES   6 A  174  PRO ARG GLY ASN ALA ASN ALA MET VAL ASP GLY LEU GLU          
SEQRES   7 A  174  GLN SER TRP THR LYS LEU ALA SER GLY ALA GLU ALA ALA          
SEQRES   8 A  174  GLN LYS ALA ALA GLN GLY PHE LEU VAL ILE ALA GLY LEU          
SEQRES   9 A  174  LYS GLY ARG THR TYR GLY HIS VAL ALA VAL VAL ILE SER          
SEQRES  10 A  174  GLY PRO LEU TYR ARG GLN LYS TYR PRO MET CYS TRP CYS          
SEQRES  11 A  174  GLY SER ILE ALA GLY ALA VAL GLY GLN SER GLN GLY LEU          
SEQRES  12 A  174  LYS SER VAL GLY GLN VAL TRP ASN ARG THR ASP ARG ASP          
SEQRES  13 A  174  ARG LEU ASN TYR TYR VAL TYR SER LEU ALA SER CYS SER          
SEQRES  14 A  174  LEU PRO ARG ALA SER                                          
SEQRES   1 B  174  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 B  174  LEU VAL PRO ARG GLY SER HIS MET ASP SER LEU ASP GLN          
SEQRES   3 B  174  CYS ILE VAL ASN ALA CYS LYS ASN SER TRP ASP LYS SER          
SEQRES   4 B  174  TYR LEU ALA GLY THR PRO ASN LYS ASP ASN CYS SER GLY          
SEQRES   5 B  174  PHE VAL GLN SER VAL ALA ALA GLU LEU GLY VAL PRO MET          
SEQRES   6 B  174  PRO ARG GLY ASN ALA ASN ALA MET VAL ASP GLY LEU GLU          
SEQRES   7 B  174  GLN SER TRP THR LYS LEU ALA SER GLY ALA GLU ALA ALA          
SEQRES   8 B  174  GLN LYS ALA ALA GLN GLY PHE LEU VAL ILE ALA GLY LEU          
SEQRES   9 B  174  LYS GLY ARG THR TYR GLY HIS VAL ALA VAL VAL ILE SER          
SEQRES  10 B  174  GLY PRO LEU TYR ARG GLN LYS TYR PRO MET CYS TRP CYS          
SEQRES  11 B  174  GLY SER ILE ALA GLY ALA VAL GLY GLN SER GLN GLY LEU          
SEQRES  12 B  174  LYS SER VAL GLY GLN VAL TRP ASN ARG THR ASP ARG ASP          
SEQRES  13 B  174  ARG LEU ASN TYR TYR VAL TYR SER LEU ALA SER CYS SER          
SEQRES  14 B  174  LEU PRO ARG ALA SER                                          
SEQRES   1 E  192  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 E  192  LEU VAL PRO ARG GLY SER HIS MET LYS LEU LEU ALA GLY          
SEQRES   3 E  192  SER PHE ALA ALA LEU PHE LEU SER LEU SER ALA GLN ALA          
SEQRES   4 E  192  ALA ASP CYS THR PHE THR GLN LEU GLU ILE VAL PRO GLN          
SEQRES   5 E  192  PHE GLY SER PRO ASN MET PHE GLY GLY GLU ASP GLU HIS          
SEQRES   6 E  192  VAL ARG VAL MET PHE SER ASN GLU ASP PRO ASN ASP ASP          
SEQRES   7 E  192  ASN PRO ASP ALA PHE PRO GLU PRO PRO VAL TYR LEU ALA          
SEQRES   8 E  192  ASP ARG ASP SER GLY ASN ASP CYS ARG ILE GLU ASP GLY          
SEQRES   9 E  192  GLY ILE TRP SER ARG GLY GLY VAL PHE LEU SER GLN ASP          
SEQRES  10 E  192  GLY ARG ARG VAL LEU MET HIS GLU PHE SER GLY SER SER          
SEQRES  11 E  192  ALA GLU LEU VAL SER TYR ASP SER ALA THR CYS LYS VAL          
SEQRES  12 E  192  VAL HIS ARG GLU ASP ILE SER GLY GLN ARG TRP ALA VAL          
SEQRES  13 E  192  ASP LYS ASP GLY LEU ARG LEU GLY GLN LYS CYS SER GLY          
SEQRES  14 E  192  GLU SER VAL ASP SER CYS ALA LYS ILE VAL LYS ARG SER          
SEQRES  15 E  192  LEU ALA PRO PHE CYS GLN THR ALA LYS LYS                      
SEQRES   1 F  192  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 F  192  LEU VAL PRO ARG GLY SER HIS MET LYS LEU LEU ALA GLY          
SEQRES   3 F  192  SER PHE ALA ALA LEU PHE LEU SER LEU SER ALA GLN ALA          
SEQRES   4 F  192  ALA ASP CYS THR PHE THR GLN LEU GLU ILE VAL PRO GLN          
SEQRES   5 F  192  PHE GLY SER PRO ASN MET PHE GLY GLY GLU ASP GLU HIS          
SEQRES   6 F  192  VAL ARG VAL MET PHE SER ASN GLU ASP PRO ASN ASP ASP          
SEQRES   7 F  192  ASN PRO ASP ALA PHE PRO GLU PRO PRO VAL TYR LEU ALA          
SEQRES   8 F  192  ASP ARG ASP SER GLY ASN ASP CYS ARG ILE GLU ASP GLY          
SEQRES   9 F  192  GLY ILE TRP SER ARG GLY GLY VAL PHE LEU SER GLN ASP          
SEQRES  10 F  192  GLY ARG ARG VAL LEU MET HIS GLU PHE SER GLY SER SER          
SEQRES  11 F  192  ALA GLU LEU VAL SER TYR ASP SER ALA THR CYS LYS VAL          
SEQRES  12 F  192  VAL HIS ARG GLU ASP ILE SER GLY GLN ARG TRP ALA VAL          
SEQRES  13 F  192  ASP LYS ASP GLY LEU ARG LEU GLY GLN LYS CYS SER GLY          
SEQRES  14 F  192  GLU SER VAL ASP SER CYS ALA LYS ILE VAL LYS ARG SER          
SEQRES  15 F  192  LEU ALA PRO PHE CYS GLN THR ALA LYS LYS                      
SEQRES   1 C  174  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 C  174  LEU VAL PRO ARG GLY SER HIS MET ASP SER LEU ASP GLN          
SEQRES   3 C  174  CYS ILE VAL ASN ALA CYS LYS ASN SER TRP ASP LYS SER          
SEQRES   4 C  174  TYR LEU ALA GLY THR PRO ASN LYS ASP ASN CYS SER GLY          
SEQRES   5 C  174  PHE VAL GLN SER VAL ALA ALA GLU LEU GLY VAL PRO MET          
SEQRES   6 C  174  PRO ARG GLY ASN ALA ASN ALA MET VAL ASP GLY LEU GLU          
SEQRES   7 C  174  GLN SER TRP THR LYS LEU ALA SER GLY ALA GLU ALA ALA          
SEQRES   8 C  174  GLN LYS ALA ALA GLN GLY PHE LEU VAL ILE ALA GLY LEU          
SEQRES   9 C  174  LYS GLY ARG THR TYR GLY HIS VAL ALA VAL VAL ILE SER          
SEQRES  10 C  174  GLY PRO LEU TYR ARG GLN LYS TYR PRO MET CYS TRP CYS          
SEQRES  11 C  174  GLY SER ILE ALA GLY ALA VAL GLY GLN SER GLN GLY LEU          
SEQRES  12 C  174  LYS SER VAL GLY GLN VAL TRP ASN ARG THR ASP ARG ASP          
SEQRES  13 C  174  ARG LEU ASN TYR TYR VAL TYR SER LEU ALA SER CYS SER          
SEQRES  14 C  174  LEU PRO ARG ALA SER                                          
SEQRES   1 D  174  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 D  174  LEU VAL PRO ARG GLY SER HIS MET ASP SER LEU ASP GLN          
SEQRES   3 D  174  CYS ILE VAL ASN ALA CYS LYS ASN SER TRP ASP LYS SER          
SEQRES   4 D  174  TYR LEU ALA GLY THR PRO ASN LYS ASP ASN CYS SER GLY          
SEQRES   5 D  174  PHE VAL GLN SER VAL ALA ALA GLU LEU GLY VAL PRO MET          
SEQRES   6 D  174  PRO ARG GLY ASN ALA ASN ALA MET VAL ASP GLY LEU GLU          
SEQRES   7 D  174  GLN SER TRP THR LYS LEU ALA SER GLY ALA GLU ALA ALA          
SEQRES   8 D  174  GLN LYS ALA ALA GLN GLY PHE LEU VAL ILE ALA GLY LEU          
SEQRES   9 D  174  LYS GLY ARG THR TYR GLY HIS VAL ALA VAL VAL ILE SER          
SEQRES  10 D  174  GLY PRO LEU TYR ARG GLN LYS TYR PRO MET CYS TRP CYS          
SEQRES  11 D  174  GLY SER ILE ALA GLY ALA VAL GLY GLN SER GLN GLY LEU          
SEQRES  12 D  174  LYS SER VAL GLY GLN VAL TRP ASN ARG THR ASP ARG ASP          
SEQRES  13 D  174  ARG LEU ASN TYR TYR VAL TYR SER LEU ALA SER CYS SER          
SEQRES  14 D  174  LEU PRO ARG ALA SER                                          
SEQRES   1 G  192  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 G  192  LEU VAL PRO ARG GLY SER HIS MET LYS LEU LEU ALA GLY          
SEQRES   3 G  192  SER PHE ALA ALA LEU PHE LEU SER LEU SER ALA GLN ALA          
SEQRES   4 G  192  ALA ASP CYS THR PHE THR GLN LEU GLU ILE VAL PRO GLN          
SEQRES   5 G  192  PHE GLY SER PRO ASN MET PHE GLY GLY GLU ASP GLU HIS          
SEQRES   6 G  192  VAL ARG VAL MET PHE SER ASN GLU ASP PRO ASN ASP ASP          
SEQRES   7 G  192  ASN PRO ASP ALA PHE PRO GLU PRO PRO VAL TYR LEU ALA          
SEQRES   8 G  192  ASP ARG ASP SER GLY ASN ASP CYS ARG ILE GLU ASP GLY          
SEQRES   9 G  192  GLY ILE TRP SER ARG GLY GLY VAL PHE LEU SER GLN ASP          
SEQRES  10 G  192  GLY ARG ARG VAL LEU MET HIS GLU PHE SER GLY SER SER          
SEQRES  11 G  192  ALA GLU LEU VAL SER TYR ASP SER ALA THR CYS LYS VAL          
SEQRES  12 G  192  VAL HIS ARG GLU ASP ILE SER GLY GLN ARG TRP ALA VAL          
SEQRES  13 G  192  ASP LYS ASP GLY LEU ARG LEU GLY GLN LYS CYS SER GLY          
SEQRES  14 G  192  GLU SER VAL ASP SER CYS ALA LYS ILE VAL LYS ARG SER          
SEQRES  15 G  192  LEU ALA PRO PHE CYS GLN THR ALA LYS LYS                      
SEQRES   1 H  192  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 H  192  LEU VAL PRO ARG GLY SER HIS MET LYS LEU LEU ALA GLY          
SEQRES   3 H  192  SER PHE ALA ALA LEU PHE LEU SER LEU SER ALA GLN ALA          
SEQRES   4 H  192  ALA ASP CYS THR PHE THR GLN LEU GLU ILE VAL PRO GLN          
SEQRES   5 H  192  PHE GLY SER PRO ASN MET PHE GLY GLY GLU ASP GLU HIS          
SEQRES   6 H  192  VAL ARG VAL MET PHE SER ASN GLU ASP PRO ASN ASP ASP          
SEQRES   7 H  192  ASN PRO ASP ALA PHE PRO GLU PRO PRO VAL TYR LEU ALA          
SEQRES   8 H  192  ASP ARG ASP SER GLY ASN ASP CYS ARG ILE GLU ASP GLY          
SEQRES   9 H  192  GLY ILE TRP SER ARG GLY GLY VAL PHE LEU SER GLN ASP          
SEQRES  10 H  192  GLY ARG ARG VAL LEU MET HIS GLU PHE SER GLY SER SER          
SEQRES  11 H  192  ALA GLU LEU VAL SER TYR ASP SER ALA THR CYS LYS VAL          
SEQRES  12 H  192  VAL HIS ARG GLU ASP ILE SER GLY GLN ARG TRP ALA VAL          
SEQRES  13 H  192  ASP LYS ASP GLY LEU ARG LEU GLY GLN LYS CYS SER GLY          
SEQRES  14 H  192  GLU SER VAL ASP SER CYS ALA LYS ILE VAL LYS ARG SER          
SEQRES  15 H  192  LEU ALA PRO PHE CYS GLN THR ALA LYS LYS                      
FORMUL   9  HOH   *341(H2 O)                                                    
HELIX    1   1 ASP A    5  TRP A   16  1                                  12    
HELIX    2   2 PRO A   25  ASP A   28  5                                   4    
HELIX    3   3 ASN A   29  LEU A   41  1                                  13    
HELIX    4   4 ASN A   49  TRP A   61  1                                  13    
HELIX    5   5 SER A   66  GLN A   76  1                                  11    
HELIX    6   6 LEU A  100  LYS A  104  5                                   5    
HELIX    7   7 GLY A  115  GLN A  119  5                                   5    
HELIX    8   8 VAL A  126  TRP A  130  1                                   5    
HELIX    9   9 THR A  133  ARG A  137  5                                   5    
HELIX   10  10 ASP B    5  TRP B   16  1                                  12    
HELIX   11  11 PRO B   25  ASP B   28  5                                   4    
HELIX   12  12 ASN B   29  LEU B   41  1                                  13    
HELIX   13  13 ASN B   49  TRP B   61  1                                  13    
HELIX   14  14 SER B   66  GLN B   76  1                                  11    
HELIX   15  15 LEU B  100  LYS B  104  5                                   5    
HELIX   16  16 GLY B  115  GLN B  119  5                                   5    
HELIX   17  17 THR B  133  ARG B  137  5                                   5    
HELIX   18  18 SER E  151  ASP E  153  5                                   3    
HELIX   19  19 LEU E  163  CYS E  167  5                                   5    
HELIX   20  20 SER F  151  ASP F  153  5                                   3    
HELIX   21  21 LEU F  163  CYS F  167  5                                   5    
HELIX   22  22 ASP C    5  LYS C   18  1                                  14    
HELIX   23  23 PRO C   25  ASP C   28  5                                   4    
HELIX   24  24 ASN C   29  LEU C   41  1                                  13    
HELIX   25  25 ASN C   49  TRP C   61  1                                  13    
HELIX   26  26 SER C   66  GLN C   76  1                                  11    
HELIX   27  27 ARG C  102  LYS C  104  5                                   3    
HELIX   28  28 GLY C  115  GLN C  119  5                                   5    
HELIX   29  29 VAL C  126  TRP C  130  1                                   5    
HELIX   30  30 ASP D    5  TRP D   16  1                                  12    
HELIX   31  31 PRO D   25  ASP D   28  5                                   4    
HELIX   32  32 ASN D   29  GLY D   42  1                                  14    
HELIX   33  33 ASN D   49  SER D   60  1                                  12    
HELIX   34  34 SER D   66  GLN D   76  1                                  11    
HELIX   35  35 LEU D  100  LYS D  104  5                                   5    
HELIX   36  36 GLY D  115  GLN D  119  5                                   5    
HELIX   37  37 GLY D  127  VAL D  129  5                                   3    
HELIX   38  38 THR D  133  ARG D  137  5                                   5    
HELIX   39  39 SER G  151  ASP G  153  5                                   3    
HELIX   40  40 LEU G  163  GLN G  168  1                                   6    
SHEET    1   A 6 THR A  62  LEU A  64  0                                        
SHEET    2   A 6 ASN A 139  VAL A 142 -1  O  TYR A 140   N  LEU A  64           
SHEET    3   A 6 VAL A  80  LEU A  84 -1  N  ILE A  81   O  TYR A 141           
SHEET    4   A 6 HIS A  91  VAL A  95 -1  O  VAL A  95   N  VAL A  80           
SHEET    5   A 6 MET A 107  CYS A 110 -1  O  TRP A 109   N  VAL A  94           
SHEET    6   A 6 SER A 120  SER A 125 -1  O  SER A 120   N  CYS A 110           
SHEET    1   B 6 THR B  62  LEU B  64  0                                        
SHEET    2   B 6 ASN B 139  VAL B 142 -1  O  VAL B 142   N  THR B  62           
SHEET    3   B 6 VAL B  80  LEU B  84 -1  N  ILE B  81   O  TYR B 141           
SHEET    4   B 6 HIS B  91  VAL B  95 -1  O  VAL B  95   N  VAL B  80           
SHEET    5   B 6 MET B 107  CYS B 110 -1  O  TRP B 109   N  VAL B  94           
SHEET    6   B 6 SER B 120  SER B 125 -1  O  SER B 120   N  CYS B 110           
SHEET    1   C 3 PHE E  24  GLN E  26  0                                        
SHEET    2   C 3 TRP E  87  SER E  95 -1  O  LEU E  94   N  THR E  25           
SHEET    3   C 3 ALA E  62  PHE E  63 -1  N  PHE E  63   O  TRP E  87           
SHEET    1   D 5 PHE E  24  GLN E  26  0                                        
SHEET    2   D 5 TRP E  87  SER E  95 -1  O  LEU E  94   N  THR E  25           
SHEET    3   D 5 ARG E 100  SER E 107 -1  O  HIS E 104   N  SER E  88           
SHEET    4   D 5 SER E 110  ASP E 117 -1  O  VAL E 114   N  MET E 103           
SHEET    5   D 5 VAL E 123  ASP E 128 -1  O  VAL E 124   N  SER E 115           
SHEET    1   E 5 VAL E  30  PRO E  31  0                                        
SHEET    2   E 5 MET E  38  GLU E  42 -1  O  GLY E  40   N  VAL E  30           
SHEET    3   E 5 VAL E  46  SER E  51 -1  O  PHE E  50   N  PHE E  39           
SHEET    4   E 5 VAL E  68  ASP E  72 -1  O  TYR E  69   N  MET E  49           
SHEET    5   E 5 ASP E  78  ILE E  81 -1  O  CYS E  79   N  LEU E  70           
SHEET    1   F 3 ARG E 133  ASP E 137  0                                        
SHEET    2   F 3 GLY E 140  CYS E 147 -1  O  ARG E 142   N  ALA E 135           
SHEET    3   F 3 CYS E 155  ARG E 161 -1  O  VAL E 159   N  LEU E 143           
SHEET    1   G 3 PHE F  24  GLN F  26  0                                        
SHEET    2   G 3 TRP F  87  SER F  95 -1  O  LEU F  94   N  THR F  25           
SHEET    3   G 3 ALA F  62  PHE F  63 -1  N  PHE F  63   O  TRP F  87           
SHEET    1   H 5 PHE F  24  GLN F  26  0                                        
SHEET    2   H 5 TRP F  87  SER F  95 -1  O  LEU F  94   N  THR F  25           
SHEET    3   H 5 ARG F 100  SER F 107 -1  O  LEU F 102   N  PHE F  93           
SHEET    4   H 5 SER F 110  ASP F 117 -1  O  TYR F 116   N  VAL F 101           
SHEET    5   H 5 VAL F 123  ASP F 128 -1  O  GLU F 127   N  LEU F 113           
SHEET    1   I 5 VAL F  30  PRO F  31  0                                        
SHEET    2   I 5 MET F  38  GLU F  42 -1  O  GLY F  40   N  VAL F  30           
SHEET    3   I 5 VAL F  46  SER F  51 -1  O  PHE F  50   N  PHE F  39           
SHEET    4   I 5 VAL F  68  ASP F  72 -1  O  TYR F  69   N  MET F  49           
SHEET    5   I 5 ASN F  77  ILE F  81 -1  O  ILE F  81   N  VAL F  68           
SHEET    1   J 3 ARG F 133  VAL F 136  0                                        
SHEET    2   J 3 LEU F 141  CYS F 147 -1  O  ARG F 142   N  ALA F 135           
SHEET    3   J 3 CYS F 155  ARG F 161 -1  O  ARG F 161   N  LEU F 141           
SHEET    1   K 6 THR C  62  LEU C  64  0                                        
SHEET    2   K 6 ASN C 139  VAL C 142 -1  O  VAL C 142   N  THR C  62           
SHEET    3   K 6 VAL C  80  LEU C  84 -1  N  GLY C  83   O  ASN C 139           
SHEET    4   K 6 HIS C  91  TYR C 101 -1  O  VAL C  95   N  VAL C  80           
SHEET    5   K 6 TYR C 105  CYS C 110 -1  O  TYR C 105   N  TYR C 101           
SHEET    6   K 6 SER C 120  SER C 125 -1  O  LYS C 124   N  CYS C 108           
SHEET    1   L 6 THR D  62  LEU D  64  0                                        
SHEET    2   L 6 ASN D 139  VAL D 142 -1  O  VAL D 142   N  THR D  62           
SHEET    3   L 6 VAL D  80  LEU D  84 -1  N  ILE D  81   O  TYR D 141           
SHEET    4   L 6 HIS D  91  VAL D  95 -1  O  VAL D  95   N  VAL D  80           
SHEET    5   L 6 MET D 107  CYS D 110 -1  O  TRP D 109   N  VAL D  94           
SHEET    6   L 6 SER D 120  SER D 125 -1  O  SER D 120   N  CYS D 110           
SHEET    1   M 3 PHE G  24  GLN G  26  0                                        
SHEET    2   M 3 TRP G  87  SER G  95 -1  O  LEU G  94   N  THR G  25           
SHEET    3   M 3 ALA G  62  PHE G  63 -1  N  PHE G  63   O  TRP G  87           
SHEET    1   N 5 PHE G  24  GLN G  26  0                                        
SHEET    2   N 5 TRP G  87  SER G  95 -1  O  LEU G  94   N  THR G  25           
SHEET    3   N 5 ARG G 100  SER G 107 -1  O  LEU G 102   N  PHE G  93           
SHEET    4   N 5 SER G 110  ASP G 117 -1  O  TYR G 116   N  VAL G 101           
SHEET    5   N 5 VAL G 123  ASP G 128 -1  O  VAL G 124   N  SER G 115           
SHEET    1   O 5 VAL G  30  PRO G  31  0                                        
SHEET    2   O 5 MET G  38  GLU G  42 -1  O  GLY G  40   N  VAL G  30           
SHEET    3   O 5 VAL G  46  SER G  51 -1  O  PHE G  50   N  PHE G  39           
SHEET    4   O 5 VAL G  68  ASP G  72 -1  O  TYR G  69   N  MET G  49           
SHEET    5   O 5 ASP G  78  ILE G  81 -1  O  CYS G  79   N  LEU G  70           
SHEET    1   P 3 ARG G 133  ASP G 137  0                                        
SHEET    2   P 3 GLY G 140  CYS G 147 -1  O  ARG G 142   N  ALA G 135           
SHEET    3   P 3 CYS G 155  ARG G 161 -1  O  VAL G 159   N  LEU G 143           
SHEET    1   Q 3 PHE H  24  GLN H  26  0                                        
SHEET    2   Q 3 TRP H  87  SER H  95 -1  O  LEU H  94   N  THR H  25           
SHEET    3   Q 3 ALA H  62  PHE H  63 -1  N  PHE H  63   O  TRP H  87           
SHEET    1   R 5 PHE H  24  GLN H  26  0                                        
SHEET    2   R 5 TRP H  87  SER H  95 -1  O  LEU H  94   N  THR H  25           
SHEET    3   R 5 ARG H 100  SER H 107 -1  O  LEU H 102   N  PHE H  93           
SHEET    4   R 5 SER H 110  ASP H 117 -1  O  GLU H 112   N  GLU H 105           
SHEET    5   R 5 VAL H 123  ASP H 128 -1  O  HIS H 125   N  SER H 115           
SHEET    1   S 5 VAL H  30  PRO H  31  0                                        
SHEET    2   S 5 MET H  38  GLU H  42 -1  O  GLY H  40   N  VAL H  30           
SHEET    3   S 5 VAL H  46  SER H  51 -1  O  PHE H  50   N  PHE H  39           
SHEET    4   S 5 VAL H  68  ASP H  72 -1  O  TYR H  69   N  MET H  49           
SHEET    5   S 5 ASN H  77  ILE H  81 -1  O  ASN H  77   N  ASP H  72           
SHEET    1   T 3 ARG H 133  VAL H 136  0                                        
SHEET    2   T 3 LEU H 141  GLY H 144 -1  O  ARG H 142   N  ALA H 135           
SHEET    3   T 3 ILE H 158  ARG H 161 -1  O  VAL H 159   N  LEU H 143           
SSBOND   1 CYS A    7    CYS A  148                          1555   1555  2.03  
SSBOND   2 CYS B    7    CYS B  148                          1555   1555  2.03  
SSBOND   3 CYS E   22    CYS E  167                          1555   1555  2.04  
SSBOND   4 CYS E   79    CYS E  121                          1555   1555  2.05  
SSBOND   5 CYS E  147    CYS E  155                          1555   1555  2.03  
SSBOND   6 CYS F   22    CYS F  167                          1555   1555  2.04  
SSBOND   7 CYS F   79    CYS F  121                          1555   1555  2.05  
SSBOND   8 CYS F  147    CYS F  155                          1555   1555  2.04  
SSBOND   9 CYS C    7    CYS C  148                          1555   1555  2.03  
SSBOND  10 CYS D    7    CYS D  148                          1555   1555  2.04  
SSBOND  11 CYS G   22    CYS G  167                          1555   1555  2.04  
SSBOND  12 CYS G   79    CYS G  121                          1555   1555  2.05  
SSBOND  13 CYS G  147    CYS G  155                          1555   1555  2.03  
SSBOND  14 CYS H   22    CYS H  167                          1555   1555  2.04  
SSBOND  15 CYS H   79    CYS H  121                          1555   1555  2.04  
SSBOND  16 CYS H  147    CYS H  155                          1555   1555  2.03  
CISPEP   1 PRO E   66    PRO E   67          0         1.88                     
CISPEP   2 GLY E   85    ILE E   86          0       -10.04                     
CISPEP   3 PRO F   66    PRO F   67          0        -3.06                     
CISPEP   4 ASP F   83    GLY F   84          0         1.16                     
CISPEP   5 GLY F   85    ILE F   86          0        -6.38                     
CISPEP   6 PRO G   66    PRO G   67          0         0.01                     
CISPEP   7 GLY G   85    ILE G   86          0       -10.80                     
CISPEP   8 PRO H   66    PRO H   67          0        -2.05                     
CISPEP   9 GLY H   85    ILE H   86          0        -5.78                     
CRYST1   97.000   97.000  292.171  90.00  90.00 120.00 P 32 2 1     24          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.010309  0.005952  0.000000        0.00000                         
SCALE2      0.000000  0.011904  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.003423        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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