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Database: PDB
Entry: 3ZDL
LinkDB: 3ZDL
Original site: 3ZDL 
HEADER    CELL ADHESION                           28-NOV-12   3ZDL              
TITLE     VINCULIN HEAD (1-258) IN COMPLEX WITH A RIAM FRAGMENT                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: VINCULIN;                                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: VD1 DOMAIN, RESIDUES 1-259;                                
COMPND   5 SYNONYM: METAVINCULIN;                                               
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B MEMBER  
COMPND   9  1-INTERACTING PROTEIN;                                              
COMPND  10 CHAIN: B;                                                            
COMPND  11 FRAGMENT: N TERMINAL DOMAIN, RESIUDES 1-32;                          
COMPND  12 SYNONYM: APBB1-INTERACTING PROTEIN 1, PROLINE-RICH EVH1 LIGAND 1,    
COMPND  13  PREL-1, PROLINE-RICH PROTEIN 73, RAP1-GTP-INTERACTING ADAPTER       
COMPND  14  MOLECULE, RIAM, RETINOIC ACID-RESPONSIVE PROLINE-RICH PROTEIN 1,    
COMPND  15  RARP-1;                                                             
COMPND  16 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: GALLUS GALLUS;                                  
SOURCE   3 ORGANISM_COMMON: CHICKEN;                                            
SOURCE   4 ORGANISM_TAXID: 9031;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_VECTOR: PET15B;                                    
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 SYNTHETIC: YES;                                                      
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606                                                 
KEYWDS    CELL ADHESION                                                         
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    T.ZACHARCHENKO,P.R.ELLIOTT,B.T.GOULT,N.BATE,D.R.CRITCHELY,            
AUTHOR   2 I.L.BARSUKOV                                                         
REVDAT   5   17-APR-13 3ZDL    1       JRNL                                     
REVDAT   4   10-APR-13 3ZDL    1       REMARK                                   
REVDAT   3   06-MAR-13 3ZDL    1       REMARK                                   
REVDAT   2   20-FEB-13 3ZDL    1       JRNL                                     
REVDAT   1   13-FEB-13 3ZDL    0                                                
JRNL        AUTH   B.T.GOULT,T.ZACHARCHENKO,N.BATE,R.TSANG,F.HEY,A.R.GINGRAS,   
JRNL        AUTH 2 P.R.ELLIOTT,G.C.ROBERTS,C.BALLESTREM,D.R.CRITCHLEY,          
JRNL        AUTH 3 I.L.BARSUKOV                                                 
JRNL        TITL   RIAM AND VINCULIN BINDING TO TALIN ARE MUTUALLY EXCLUSIVE    
JRNL        TITL 2 AND REGULATE ADHESION ASSEMBLY AND TURNOVER.                 
JRNL        REF    J.BIOL.CHEM.                  V. 288  8238 2013              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   23389036                                                     
JRNL        DOI    10.1074/JBC.M112.438119                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.30 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8_1069)                     
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.300                          
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 47.800                         
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.34                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.88                          
REMARK   3   NUMBER OF REFLECTIONS             : 16016                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.1901                          
REMARK   3   R VALUE            (WORKING SET) : 0.1881                          
REMARK   3   FREE R VALUE                     : 0.2298                          
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.0                             
REMARK   3   FREE R VALUE TEST SET COUNT      : 801                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 47.8104 -  4.1786    1.00     2675   134  0.1844 0.2080        
REMARK   3     2  4.1786 -  3.3170    1.00     2552   130  0.1676 0.1895        
REMARK   3     3  3.3170 -  2.8977    1.00     2516   136  0.1831 0.2637        
REMARK   3     4  2.8977 -  2.6328    1.00     2494   148  0.1991 0.2492        
REMARK   3     5  2.6328 -  2.4441    1.00     2487   144  0.2094 0.2582        
REMARK   3     6  2.4441 -  2.3000    1.00     2491   109  0.2423 0.3490        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.29             
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 23.51            
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 34.31                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.008           2230                                  
REMARK   3   ANGLE     :  1.090           3018                                  
REMARK   3   CHIRALITY :  0.064            371                                  
REMARK   3   PLANARITY :  0.005            384                                  
REMARK   3   DIHEDRAL  : 15.857            852                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 2                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 1:256)                              
REMARK   3    ORIGIN FOR THE GROUP (A): -11.0375 -13.6938  23.4492              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1189 T22:   0.1473                                     
REMARK   3      T33:   0.1037 T12:   0.0212                                     
REMARK   3      T13:   0.0176 T23:   0.0381                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7701 L22:   0.6440                                     
REMARK   3      L33:   1.0889 L12:  -0.5335                                     
REMARK   3      L13:   1.1664 L23:  -0.6836                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0898 S12:   0.1576 S13:  -0.0243                       
REMARK   3      S21:  -0.1971 S22:  -0.0721 S23:  -0.0355                       
REMARK   3      S31:   0.0506 S32:   0.0742 S33:   0.0112                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: (CHAIN B AND RESID 7:32)                               
REMARK   3    ORIGIN FOR THE GROUP (A): -11.5667 -27.7400  43.7533              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3363 T22:   0.2054                                     
REMARK   3      T33:   0.7093 T12:  -0.0166                                     
REMARK   3      T13:  -0.1659 T23:   0.0275                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0156 L22:   2.2228                                     
REMARK   3      L33:   3.5701 L12:   0.3822                                     
REMARK   3      L13:  -0.4105 L23:  -0.8601                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0662 S12:  -0.0446 S13:  -0.0838                       
REMARK   3      S21:   0.0424 S22:   0.2203 S23:   0.0795                       
REMARK   3      S31:   0.2479 S32:  -0.4066 S33:   0.0889                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3ZDL COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 14-DEC-12.                  
REMARK 100 THE PDBE ID CODE IS EBI-54919.                                       
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 07-OCT-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : DIAMOND                            
REMARK 200  BEAMLINE                       : I24                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9778                             
REMARK 200  MONOCHROMATOR                  : ACCEL FIXED EXIT DOUBLE            
REMARK 200                                   CRYSTAL                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL (PILATUS 6M)                 
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS                            
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : IMOSFLM                            
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 16055                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.30                               
REMARK 200  RESOLUTION RANGE LOW       (A) : 56.49                              
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.9                                
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 5.6                                
REMARK 200  R MERGE                    (I) : 0.11                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 7.80                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.42                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.5                                
REMARK 200  R MERGE FOR SHELL          (I) : 0.87                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 3.00                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1XWJ                                       
REMARK 200                                                                      
REMARK 200 REMARK: NONE                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 55.96                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.79                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 1.2M AMMONIUM SULPHATE,                  
REMARK 280  0.05M TRI-SODIUM CITRATE, 3% ISOPROPANOL                            
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       25.86500            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       35.01500            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       25.86500            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       35.01500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2280 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 14660 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -27.8 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   -21                                                      
REMARK 465     GLY A   -20                                                      
REMARK 465     SER A   -19                                                      
REMARK 465     SER A   -18                                                      
REMARK 465     HIS A   -17                                                      
REMARK 465     HIS A   -16                                                      
REMARK 465     HIS A   -15                                                      
REMARK 465     HIS A   -14                                                      
REMARK 465     HIS A   -13                                                      
REMARK 465     HIS A   -12                                                      
REMARK 465     SER A   -11                                                      
REMARK 465     SER A   -10                                                      
REMARK 465     GLY A    -9                                                      
REMARK 465     LEU A    -8                                                      
REMARK 465     VAL A    -7                                                      
REMARK 465     PRO A    -6                                                      
REMARK 465     ARG A    -5                                                      
REMARK 465     GLY A    -4                                                      
REMARK 465     SER A    -3                                                      
REMARK 465     HIS A    -2                                                      
REMARK 465     MET A    -1                                                      
REMARK 465     ALA A   256                                                      
REMARK 465     TRP A   257                                                      
REMARK 465     ALA A   258                                                      
REMARK 465     MET B     1                                                      
REMARK 465     GLY B     2                                                      
REMARK 465     GLU B     3                                                      
REMARK 465     SER B     4                                                      
REMARK 465     SER B     5                                                      
REMARK 465     GLU B     6                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   O    TYR B    32     O    HOH A  2027     4446     2.16            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999 C TERMINAL TYROSINE NON NATIVE                                       
DBREF  3ZDL A    0   258  UNP    P12003   VINC_CHICK       1    259             
DBREF  3ZDL B    1    32  UNP    Q7Z5R6   AB1IP_HUMAN      1     32             
SEQADV 3ZDL MET A  -21  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL GLY A  -20  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL SER A  -19  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL SER A  -18  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A  -17  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A  -16  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A  -15  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A  -14  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A  -13  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A  -12  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL SER A  -11  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL SER A  -10  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL GLY A   -9  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL LEU A   -8  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL VAL A   -7  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL PRO A   -6  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL ARG A   -5  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL GLY A   -4  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL SER A   -3  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL HIS A   -2  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL MET A   -1  UNP  P12003              EXPRESSION TAG                 
SEQADV 3ZDL TYR B   32  UNP  Q8IYL7    PRO    32 ENGINEERED MUTATION            
SEQRES   1 A  280  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 A  280  LEU VAL PRO ARG GLY SER HIS MET MET PRO VAL PHE HIS          
SEQRES   3 A  280  THR ARG THR ILE GLU SER ILE LEU GLU PRO VAL ALA GLN          
SEQRES   4 A  280  GLN ILE SER HIS LEU VAL ILE MET HIS GLU GLU GLY GLU          
SEQRES   5 A  280  VAL ASP GLY LYS ALA ILE PRO ASP LEU THR ALA PRO VAL          
SEQRES   6 A  280  SER ALA VAL GLN ALA ALA VAL SER ASN LEU VAL ARG VAL          
SEQRES   7 A  280  GLY LYS GLU THR VAL GLN THR THR GLU ASP GLN ILE LEU          
SEQRES   8 A  280  LYS ARG ASP MET PRO PRO ALA PHE ILE LYS VAL GLU ASN          
SEQRES   9 A  280  ALA CYS THR LYS LEU VAL ARG ALA ALA GLN MET LEU GLN          
SEQRES  10 A  280  ALA ASP PRO TYR SER VAL PRO ALA ARG ASP TYR LEU ILE          
SEQRES  11 A  280  ASP GLY SER ARG GLY ILE LEU SER GLY THR SER ASP LEU          
SEQRES  12 A  280  LEU LEU THR PHE ASP GLU ALA GLU VAL ARG LYS ILE ILE          
SEQRES  13 A  280  ARG VAL CYS LYS GLY ILE LEU GLU TYR LEU THR VAL ALA          
SEQRES  14 A  280  GLU VAL VAL GLU THR MET GLU ASP LEU VAL THR TYR THR          
SEQRES  15 A  280  LYS ASN LEU GLY PRO GLY MET THR LYS MET ALA LYS MET          
SEQRES  16 A  280  ILE ASP GLU ARG GLN GLN GLU LEU THR HIS GLN GLU HIS          
SEQRES  17 A  280  ARG VAL MET LEU VAL ASN SER MET ASN THR VAL LYS GLU          
SEQRES  18 A  280  LEU LEU PRO VAL LEU ILE SER ALA MET LYS ILE PHE VAL          
SEQRES  19 A  280  THR THR LYS ASN THR LYS SER GLN GLY ILE GLU GLU ALA          
SEQRES  20 A  280  LEU LYS ASN ARG ASN PHE THR VAL GLU LYS MET SER ALA          
SEQRES  21 A  280  GLU ILE ASN GLU ILE ILE ARG VAL LEU GLN LEU THR SER          
SEQRES  22 A  280  TRP ASP GLU ASP ALA TRP ALA                                  
SEQRES   1 B   32  MET GLY GLU SER SER GLU ASP ILE ASP GLN MET PHE SER          
SEQRES   2 B   32  THR LEU LEU GLY GLU MET ASP LEU LEU THR GLN SER LEU          
SEQRES   3 B   32  GLY VAL ASP THR LEU TYR                                      
FORMUL   3  HOH   *57(H2 O)                                                     
HELIX    1   1 THR A    5  GLU A   27  1                                  23    
HELIX    2   2 GLU A   28  GLY A   33  1                                   6    
HELIX    3   3 LEU A   39  THR A   64  1                                  26    
HELIX    4   4 ASP A   66  ASP A   97  1                                  32    
HELIX    5   5 SER A  100  ALA A  147  1                                  48    
HELIX    6   6 GLU A  148  VAL A  150  5                                   3    
HELIX    7   7 THR A  152  LEU A  181  1                                  30    
HELIX    8   8 HIS A  183  THR A  217  1                                  35    
HELIX    9   9 GLY A  221  LEU A  247  1                                  27    
HELIX   10  10 SER A  251  ASP A  255  5                                   5    
HELIX   11  11 ASP B    7  LEU B   26  1                                  20    
CRYST1   51.730   70.030   95.600  90.00  90.00  90.00 P 21 21 2     4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.019331  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.014280  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010460        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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