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Database: PDB
Entry: 3ZEE
LinkDB: 3ZEE
Original site: 3ZEE 
HEADER    CELL CYCLE                              05-DEC-12   3ZEE              
TITLE     ELECTRON CYRO-MICROSCOPY HELICAL RECONSTRUCTION OF PAR-3 N TERMINAL   
TITLE    2 DOMAIN                                                               
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PARTITIONING DEFECTIVE 3 HOMOLOG;                          
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: N-TERMINAL DUF3534 DOMAIN, RESIDUES 2-82;                  
COMPND   5 SYNONYM: PAR-3, PARD-3, ATYPICAL PKC ISOTYPE-SPECIFIC-INTERACTING    
COMPND   6 PROTEIN, ASIP, ATYPICAL PKC-SPECIFIC-BINDING PROTEIN, ASBP;          
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE   3 ORGANISM_COMMON: NORWAY RAT;                                         
SOURCE   4 ORGANISM_TAXID: 10116;                                               
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   8 EXPRESSION_SYSTEM_VARIANT: CODONPLUS;                                
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_VECTOR: PET32A                                     
KEYWDS    CELL CYCLE                                                            
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    Y.ZHANG,W.WANG,J.CHEN,K.ZHANG,F.GAO,W.GONG,M.ZHANG,F.SUN,W.FENG       
REVDAT   2   30-AUG-17 3ZEE    1       REMARK ATOM                              
REVDAT   1   16-OCT-13 3ZEE    0                                                
JRNL        AUTH   Y.ZHANG,W.WANG,J.CHEN,K.ZHANG,F.GAO,B.GAO,S.ZHANG,M.DONG,    
JRNL        AUTH 2 F.BESENBACHER,W.GONG,M.ZHANG,F.SUN,W.FENG                    
JRNL        TITL   STRUCTURAL INSIGHTS INTO THE INTRINSIC SELF-ASSEMBLY OF      
JRNL        TITL 2 PAR-3 N-TERMINAL DOMAIN.                                     
JRNL        REF    STRUCTURE                     V.  21   997 2013              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   23643951                                                     
JRNL        DOI    10.1016/J.STR.2013.04.004                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    6.10 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : NAMD, VMD, IMAGIC                         
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : 4I6P                                
REMARK   3   REFINEMENT SPACE             : REAL                                
REMARK   3   REFINEMENT PROTOCOL          : OTHER                               
REMARK   3   REFINEMENT TARGET            : ENERGY FUNCTION IN NAMD2            
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : METHOD--CROSS CORRELATION REFINEMENT PROTOCOL-   
REMARK   3  -X-RAY                                                              
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 6.100                          
REMARK   3   NUMBER OF PARTICLES               : 84000                          
REMARK   3   CTF CORRECTION METHOD             : NULL                           
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: THE INITIAL MODEL WAS OBTAINED USING IHRSR. THEN      
REMARK   3  THE FINAL RECONSTRUCTION WERE OBTAINED BY PROJECTION MATCHING IN    
REMARK   3  EMAN. THE RESOLUTION CRITERIA USED WAS GOLDEN CRITERIA FSC 0.5.     
REMARK   3  SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2237.           
REMARK   3  (DEPOSITION ID: 11249).                                             
REMARK   4                                                                      
REMARK   4 3ZEE COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE.                               
REMARK 100 THE DEPOSITION ID IS D_1290054961.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : HELICAL                           
REMARK 245   SPECIMEN TYPE                  : VITREOUS ICE OF CRYO EM           
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : FILAMENT                          
REMARK 245   PARTICLE TYPE                  : HELICAL                           
REMARK 245   NAME OF SAMPLE                 : PAR-3 N-TERMINAL DUF3534 DOMAIN   
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 2.00                              
REMARK 245   SAMPLE SUPPORT DETAILS         : CARBON                            
REMARK 245   SAMPLE VITRIFICATION DETAILS   : LIQUID ETHANE                     
REMARK 245   SAMPLE BUFFER                  : 50 MM TRIS, 100 MM NACL, 1 MM     
REMARK 245                                    DTT AND 1 MM EDTA                 
REMARK 245   PH                             : 8.00                              
REMARK 245   SAMPLE DETAILS                 : SUPPORTING FILM IS GIG HOLELY     
REMARK 245  GRID.                                                               
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : 01-DEC-10                      
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : 95.00                          
REMARK 245   MICROSCOPE MODEL                  : FEI TITAN KRIOS                
REMARK 245   DETECTOR TYPE                     : GATAN ULTRASCAN 4000 (4K X     
REMARK 245                                       4K)                            
REMARK 245   MINIMUM DEFOCUS (NM)              : 1800.00                        
REMARK 245   MAXIMUM DEFOCUS (NM)              : 2500.00                        
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : 2.70                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 20.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : 96000                          
REMARK 245   CALIBRATED MAGNIFICATION          : NULL                           
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 300                            
REMARK 245   IMAGING DETAILS                   : THE MICROSCOPE MODEL IS FEI    
REMARK 245  TITAN KRIOS. 6460 RAW IMAGES WERE COLLECTED AUTOMATICALLY USING     
REMARK 245  THE PACKAGE LEGINON. GOOD MICROGRAPHS WERE SELECTED ONE BY ONE      
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 300 THE ASSEMBLY REPRESENTED IN THIS ENTRY HAS REGULAR                   
REMARK 300 HELICAL SYMMETRY WITH THE FOLLOWING PARAMETERS:                      
REMARK 300 ROTATION PER SUBUNIT (TWIST) = -43.84 DEGREES                        
REMARK 300 RISE PER SUBUNIT (HEIGHT) = 3.53 ANGSTROMS                           
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  0.883275  0.468855  0.000000        0.00000            
REMARK 350   BIOMT2   1 -0.468855  0.883275  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000      -84.76800            
REMARK 350   BIOMT1   2  0.312418  0.949945  0.000000        0.00000            
REMARK 350   BIOMT2   2 -0.949945  0.312418  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000  1.000000      -81.23600            
REMARK 350   BIOMT1   3 -0.432558  0.901606  0.000000        0.00000            
REMARK 350   BIOMT2   3 -0.901606 -0.432558  0.000000        0.00000            
REMARK 350   BIOMT3   3  0.000000  0.000000  1.000000      -77.70400            
REMARK 350   BIOMT1   4 -0.936458  0.350780  0.000000        0.00000            
REMARK 350   BIOMT2   4 -0.350780 -0.936458  0.000000        0.00000            
REMARK 350   BIOMT3   4  0.000000  0.000000  1.000000      -74.17200            
REMARK 350   BIOMT1   5 -0.918446 -0.395545  0.000000        0.00000            
REMARK 350   BIOMT2   5  0.395545 -0.918446  0.000000        0.00000            
REMARK 350   BIOMT3   5  0.000000  0.000000  1.000000      -70.64000            
REMARK 350   BIOMT1   6 -0.388561 -0.921423  0.000000        0.00000            
REMARK 350   BIOMT2   6  0.921423 -0.388561  0.000000        0.00000            
REMARK 350   BIOMT3   6  0.000000  0.000000  1.000000      -67.10800            
REMARK 350   BIOMT1   7  0.357879 -0.933768  0.000000        0.00000            
REMARK 350   BIOMT2   7  0.933768  0.357879  0.000000        0.00000            
REMARK 350   BIOMT3   7  0.000000  0.000000  1.000000      -63.57600            
REMARK 350   BIOMT1   8  0.904864 -0.425700  0.000000        0.00000            
REMARK 350   BIOMT2   8  0.425700  0.904864  0.000000        0.00000            
REMARK 350   BIOMT3   8  0.000000  0.000000  1.000000      -60.04400            
REMARK 350   BIOMT1   9  0.947546  0.319621  0.000000        0.00000            
REMARK 350   BIOMT2   9 -0.319621  0.947546  0.000000        0.00000            
REMARK 350   BIOMT3   9  0.000000  0.000000  1.000000      -56.51200            
REMARK 350   BIOMT1  10  0.462136  0.886809  0.000000        0.00000            
REMARK 350   BIOMT2  10 -0.886809  0.462136  0.000000        0.00000            
REMARK 350   BIOMT3  10  0.000000  0.000000  1.000000      -52.98000            
REMARK 350   BIOMT1  11 -0.280834  0.959756  0.000000        0.00000            
REMARK 350   BIOMT2  11 -0.959756 -0.280834  0.000000        0.00000            
REMARK 350   BIOMT3  11  0.000000  0.000000  1.000000      -49.44800            
REMARK 350   BIOMT1  12 -0.867288  0.497807  0.000000        0.00000            
REMARK 350   BIOMT2  12 -0.497807 -0.867288  0.000000        0.00000            
REMARK 350   BIOMT3  12  0.000000  0.000000  1.000000      -45.91600            
REMARK 350   BIOMT1  13 -0.970380 -0.241583  0.000000        0.00000            
REMARK 350   BIOMT2  13  0.241583 -0.970380  0.000000        0.00000            
REMARK 350   BIOMT3  13  0.000000  0.000000  1.000000      -42.38400            
REMARK 350   BIOMT1  14 -0.532655 -0.846333  0.000000        0.00000            
REMARK 350   BIOMT2  14  0.846333 -0.532655  0.000000        0.00000            
REMARK 350   BIOMT3  14  0.000000  0.000000  1.000000      -38.85200            
REMARK 350   BIOMT1  15  0.201933 -0.979399  0.000000        0.00000            
REMARK 350   BIOMT2  15  0.979399  0.201933  0.000000        0.00000            
REMARK 350   BIOMT3  15  0.000000  0.000000  1.000000      -35.32000            
REMARK 350   BIOMT1  16  0.823978 -0.566622  0.000000        0.00000            
REMARK 350   BIOMT2  16  0.566622  0.823978  0.000000        0.00000            
REMARK 350   BIOMT3  16  0.000000  0.000000  1.000000      -31.78800            
REMARK 350   BIOMT1  17  0.986799  0.161948  0.000000        0.00000            
REMARK 350   BIOMT2  17 -0.161948  0.986799  0.000000        0.00000            
REMARK 350   BIOMT3  17  0.000000  0.000000  1.000000      -28.25600            
REMARK 350   BIOMT1  18  0.599652  0.800261  0.000000        0.00000            
REMARK 350   BIOMT2  18 -0.800261  0.599652  0.000000        0.00000            
REMARK 350   BIOMT3  18  0.000000  0.000000  1.000000      -24.72400            
REMARK 350   BIOMT1  19 -0.121696  0.992567  0.000000        0.00000            
REMARK 350   BIOMT2  19 -0.992567 -0.121696  0.000000        0.00000            
REMARK 350   BIOMT3  19  0.000000  0.000000  1.000000      -21.19200            
REMARK 350   BIOMT1  20 -0.775220  0.631691  0.000000        0.00000            
REMARK 350   BIOMT2  20 -0.631691 -0.775220  0.000000        0.00000            
REMARK 350   BIOMT3  20  0.000000  0.000000  1.000000      -17.66000            
REMARK 350   BIOMT1  21 -0.996694 -0.081243  0.000000        0.00000            
REMARK 350   BIOMT2  21  0.081243 -0.996694  0.000000        0.00000            
REMARK 350   BIOMT3  21  0.000000  0.000000  1.000000      -14.12800            
REMARK 350   BIOMT1  22 -0.662685 -0.748898  0.000000        0.00000            
REMARK 350   BIOMT2  22  0.748898 -0.662685  0.000000        0.00000            
REMARK 350   BIOMT3  22  0.000000  0.000000  1.000000      -10.59600            
REMARK 350   BIOMT1  23  0.040655 -0.999173  0.000000        0.00000            
REMARK 350   BIOMT2  23  0.999173  0.040655  0.000000        0.00000            
REMARK 350   BIOMT3  23  0.000000  0.000000  1.000000       -7.06400            
REMARK 350   BIOMT1  24  0.721337 -0.692584  0.000000        0.00000            
REMARK 350   BIOMT2  24  0.692584  0.721337  0.000000        0.00000            
REMARK 350   BIOMT3  24  0.000000  0.000000  1.000000       -3.53200            
REMARK 350   BIOMT1  25  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2  25  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3  25  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1  26  0.721337  0.692584  0.000000        0.00000            
REMARK 350   BIOMT2  26 -0.692584  0.721337  0.000000        0.00000            
REMARK 350   BIOMT3  26  0.000000  0.000000  1.000000        3.53200            
REMARK 350   BIOMT1  27  0.040655  0.999173  0.000000        0.00000            
REMARK 350   BIOMT2  27 -0.999173  0.040655  0.000000        0.00000            
REMARK 350   BIOMT3  27  0.000000  0.000000  1.000000        7.06400            
REMARK 350   BIOMT1  28 -0.662685  0.748898  0.000000        0.00000            
REMARK 350   BIOMT2  28 -0.748898 -0.662685  0.000000        0.00000            
REMARK 350   BIOMT3  28  0.000000  0.000000  1.000000       10.59600            
REMARK 350   BIOMT1  29 -0.996694  0.081243  0.000000        0.00000            
REMARK 350   BIOMT2  29 -0.081243 -0.996694  0.000000        0.00000            
REMARK 350   BIOMT3  29  0.000000  0.000000  1.000000       14.12800            
REMARK 350   BIOMT1  30 -0.775220 -0.631691  0.000000        0.00000            
REMARK 350   BIOMT2  30  0.631691 -0.775220  0.000000        0.00000            
REMARK 350   BIOMT3  30  0.000000  0.000000  1.000000       17.66000            
REMARK 350   BIOMT1  31 -0.121696 -0.992567  0.000000        0.00000            
REMARK 350   BIOMT2  31  0.992567 -0.121696  0.000000        0.00000            
REMARK 350   BIOMT3  31  0.000000  0.000000  1.000000       21.19200            
REMARK 350   BIOMT1  32  0.599652 -0.800261  0.000000        0.00000            
REMARK 350   BIOMT2  32  0.800261  0.599652  0.000000        0.00000            
REMARK 350   BIOMT3  32  0.000000  0.000000  1.000000       24.72400            
REMARK 350   BIOMT1  33  0.986799 -0.161948  0.000000        0.00000            
REMARK 350   BIOMT2  33  0.161948  0.986799  0.000000        0.00000            
REMARK 350   BIOMT3  33  0.000000  0.000000  1.000000       28.25600            
REMARK 350   BIOMT1  34  0.823978  0.566622  0.000000        0.00000            
REMARK 350   BIOMT2  34 -0.566622  0.823978  0.000000        0.00000            
REMARK 350   BIOMT3  34  0.000000  0.000000  1.000000       31.78800            
REMARK 350   BIOMT1  35  0.201933  0.979399  0.000000        0.00000            
REMARK 350   BIOMT2  35 -0.979399  0.201933  0.000000        0.00000            
REMARK 350   BIOMT3  35  0.000000  0.000000  1.000000       35.32000            
REMARK 350   BIOMT1  36 -0.532655  0.846333  0.000000        0.00000            
REMARK 350   BIOMT2  36 -0.846333 -0.532655  0.000000        0.00000            
REMARK 350   BIOMT3  36  0.000000  0.000000  1.000000       38.85200            
REMARK 350   BIOMT1  37 -0.970380  0.241583  0.000000        0.00000            
REMARK 350   BIOMT2  37 -0.241583 -0.970380  0.000000        0.00000            
REMARK 350   BIOMT3  37  0.000000  0.000000  1.000000       42.38400            
REMARK 350   BIOMT1  38 -0.867288 -0.497807  0.000000        0.00000            
REMARK 350   BIOMT2  38  0.497807 -0.867288  0.000000        0.00000            
REMARK 350   BIOMT3  38  0.000000  0.000000  1.000000       45.91600            
REMARK 350   BIOMT1  39 -0.280834 -0.959756  0.000000        0.00000            
REMARK 350   BIOMT2  39  0.959756 -0.280834  0.000000        0.00000            
REMARK 350   BIOMT3  39  0.000000  0.000000  1.000000       49.44800            
REMARK 350   BIOMT1  40  0.462136 -0.886809  0.000000        0.00000            
REMARK 350   BIOMT2  40  0.886809  0.462136  0.000000        0.00000            
REMARK 350   BIOMT3  40  0.000000  0.000000  1.000000       52.98000            
REMARK 350   BIOMT1  41  0.947546 -0.319621  0.000000        0.00000            
REMARK 350   BIOMT2  41  0.319621  0.947546  0.000000        0.00000            
REMARK 350   BIOMT3  41  0.000000  0.000000  1.000000       56.51200            
REMARK 350   BIOMT1  42  0.904864  0.425700  0.000000        0.00000            
REMARK 350   BIOMT2  42 -0.425700  0.904864  0.000000        0.00000            
REMARK 350   BIOMT3  42  0.000000  0.000000  1.000000       60.04400            
REMARK 350   BIOMT1  43  0.357879  0.933768  0.000000        0.00000            
REMARK 350   BIOMT2  43 -0.933768  0.357879  0.000000        0.00000            
REMARK 350   BIOMT3  43  0.000000  0.000000  1.000000       63.57600            
REMARK 350   BIOMT1  44 -0.388561  0.921423  0.000000        0.00000            
REMARK 350   BIOMT2  44 -0.921423 -0.388561  0.000000        0.00000            
REMARK 350   BIOMT3  44  0.000000  0.000000  1.000000       67.10800            
REMARK 350   BIOMT1  45 -0.918446  0.395545  0.000000        0.00000            
REMARK 350   BIOMT2  45 -0.395545 -0.918446  0.000000        0.00000            
REMARK 350   BIOMT3  45  0.000000  0.000000  1.000000       70.64000            
REMARK 350   BIOMT1  46 -0.936458 -0.350780  0.000000        0.00000            
REMARK 350   BIOMT2  46  0.350780 -0.936458  0.000000        0.00000            
REMARK 350   BIOMT3  46  0.000000  0.000000  1.000000       74.17200            
REMARK 350   BIOMT1  47 -0.432558 -0.901606  0.000000        0.00000            
REMARK 350   BIOMT2  47  0.901606 -0.432558  0.000000        0.00000            
REMARK 350   BIOMT3  47  0.000000  0.000000  1.000000       77.70400            
REMARK 350   BIOMT1  48  0.312418 -0.949945  0.000000        0.00000            
REMARK 350   BIOMT2  48  0.949945  0.312418  0.000000        0.00000            
REMARK 350   BIOMT3  48  0.000000  0.000000  1.000000       81.23600            
REMARK 350   BIOMT1  49  0.883275 -0.468855  0.000000        0.00000            
REMARK 350   BIOMT2  49  0.468855  0.883275  0.000000        0.00000            
REMARK 350   BIOMT3  49  0.000000  0.000000  1.000000       84.76800            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    LEU A  59   CA    LEU A  59   C      -0.160                       
REMARK 500    ARG A  74   CD    ARG A  74   NE      0.105                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    VAL A   5   CA  -  CB  -  CG2 ANGL. DEV. = -12.4 DEGREES          
REMARK 500    ARG A  11   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.5 DEGREES          
REMARK 500    VAL A  13   CA  -  CB  -  CG1 ANGL. DEV. =   9.7 DEGREES          
REMARK 500    PHE A  24   CB  -  CG  -  CD1 ANGL. DEV. =  -5.7 DEGREES          
REMARK 500    ARG A  33   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500    TYR A  34   CB  -  CG  -  CD2 ANGL. DEV. =  -8.4 DEGREES          
REMARK 500    TYR A  34   CB  -  CG  -  CD1 ANGL. DEV. =   5.1 DEGREES          
REMARK 500    ARG A  35   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES          
REMARK 500    TYR A  44   CG  -  CD2 -  CE2 ANGL. DEV. =  -5.4 DEGREES          
REMARK 500    ARG A  50   NE  -  CZ  -  NH2 ANGL. DEV. =   3.2 DEGREES          
REMARK 500    ASP A  55   CB  -  CG  -  OD1 ANGL. DEV. =   6.5 DEGREES          
REMARK 500    VAL A  78   C   -  N   -  CA  ANGL. DEV. =  16.8 DEGREES          
REMARK 500    ASP A  80   CB  -  CA  -  C   ANGL. DEV. =  12.1 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    MET A  21      156.27    176.89                                   
REMARK 500    VAL A  38       -3.24   -148.23                                   
REMARK 500    LYS A  40     -112.69    -89.41                                   
REMARK 500    ASN A  43      116.90    179.22                                   
REMARK 500    TYR A  44     -164.72    175.14                                   
REMARK 500    ILE A  46       49.43   -145.30                                   
REMARK 500    ASP A  62      -37.49   -146.38                                   
REMARK 500    VAL A  68      -76.01   -100.87                                   
REMARK 500    ASP A  70     -157.07    -94.38                                   
REMARK 500    GLU A  81        7.25   -161.67                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 PHE A   79     ASP A   80                  149.42                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500    ARG A  20         0.13    SIDE CHAIN                              
REMARK 500    PHE A  24         0.09    SIDE CHAIN                              
REMARK 500    TYR A  34         0.10    SIDE CHAIN                              
REMARK 500    TYR A  44         0.16    SIDE CHAIN                              
REMARK 500    ARG A  50         0.08    SIDE CHAIN                              
REMARK 500    ARG A  74         0.08    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY                                       
REMARK 500                                                                      
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY                       
REMARK 500 TORSION ANGLE, C(I) - CA(I) - N(I+1) - O(I), GREATER                 
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;                     
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;                            
REMARK 500 I=INSERTION CODE).                                                   
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        ANGLE                                           
REMARK 500    PHE A   1        -10.75                                           
REMARK 500    PHE A  79        -10.34                                           
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-2237   RELATED DB: EMDB                              
REMARK 900 ELECTRON CYRO-MICROSCOPY HELICAL RECONSTRUCTION OF PAR-3 N-TERMINAL  
REMARK 900 DOMAIN                                                               
DBREF  3ZEE A    2    82  UNP    Q9Z340   PARD3_RAT        2     82             
SEQADV 3ZEE SER A   -1  UNP  Q9Z340              EXPRESSION TAG                 
SEQADV 3ZEE GLU A    0  UNP  Q9Z340              EXPRESSION TAG                 
SEQADV 3ZEE PHE A    1  UNP  Q9Z340              EXPRESSION TAG                 
SEQRES   1 A   84  SER GLU PHE LYS VAL THR VAL CYS PHE GLY ARG THR ARG          
SEQRES   2 A   84  VAL VAL VAL PRO CYS GLY ASP GLY ARG MET LYS VAL PHE          
SEQRES   3 A   84  SER LEU ILE GLN GLN ALA VAL THR ARG TYR ARG LYS ALA          
SEQRES   4 A   84  VAL ALA LYS ASP PRO ASN TYR TRP ILE GLN VAL HIS ARG          
SEQRES   5 A   84  LEU GLU HIS GLY ASP GLY GLY ILE LEU ASP LEU ASP ASP          
SEQRES   6 A   84  ILE LEU CYS ASP VAL ALA ASP ASP LYS ASP ARG LEU VAL          
SEQRES   7 A   84  ALA VAL PHE ASP GLU GLN                                      
HELIX    1   1 VAL A   23  ALA A   39  1                                  17    
HELIX    2   2 ILE A   64  ALA A   69  1                                   6    
SHEET    1  AA 2 LYS A   2  VAL A   5  0                                        
SHEET    2  AA 2 VAL A  12  PRO A  15 -1  O  VAL A  12   N  VAL A   5           
SHEET    1  AB 2 GLN A  47  GLU A  52  0                                        
SHEET    2  AB 2 VAL A  76  ASP A  80 -1  O  VAL A  76   N  GLU A  52           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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