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Database: PDB
Entry: 3ZLK
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HEADER    TRANSFERASE                             01-FEB-13   3ZLK              
TITLE     PSEUDOMONAS AERUGINOSA RMLA IN COMPLEX WITH ALLOSTERIC INHIBITOR      
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE;                 
COMPND   3 CHAIN: A, B, C, D;                                                   
COMPND   4 EC: 2.7.7.74;                                                        
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA PAO1;                    
SOURCE   3 ORGANISM_TAXID: 208964;                                              
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   6 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   8 EXPRESSION_SYSTEM_VECTOR: PET23A MODIFIED                            
KEYWDS    TRANSFERASE                                                           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.S.ALPHEY,L.PIRRIE,L.S.TORRIE,M.GARDINER,A.SARKAR,R.BRENK,           
AUTHOR   2 N.J.WESTWOOD,D.GRAY,J.H.NAISMITH                                     
REVDAT   4   07-FEB-18 3ZLK    1       JRNL                                     
REVDAT   3   05-JUL-17 3ZLK    1       REMARK                                   
REVDAT   2   06-MAR-13 3ZLK    1       JRNL                                     
REVDAT   1   20-FEB-13 3ZLK    0                                                
SPRSDE     20-FEB-13 3ZLK      4ASP                                             
JRNL        AUTH   M.S.ALPHEY,L.PIRRIE,L.S.TORRIE,W.A.BOULKEROUA,M.GARDINER,    
JRNL        AUTH 2 A.SARKAR,M.MARINGER,W.OEHLMANN,R.BRENK,M.S.SCHERMAN,         
JRNL        AUTH 3 M.MCNEIL,M.REJZEK,R.A.FIELD,M.SINGH,D.GRAY,N.J.WESTWOOD,     
JRNL        AUTH 4 J.H.NAISMITH                                                 
JRNL        TITL   ALLOSTERIC COMPETITIVE INHIBITORS OF THE GLUCOSE-1-PHOSPHATE 
JRNL        TITL 2 THYMIDYLYLTRANSFERASE (RMLA) FROM PSEUDOMONAS AERUGINOSA.    
JRNL        REF    ACS CHEM. BIOL.               V.   8   387 2013              
JRNL        REFN                   ESSN 1554-8937                               
JRNL        PMID   23138692                                                     
JRNL        DOI    10.1021/CB300426U                                            
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.95 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.6.0017                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.95                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 37.23                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 84.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 80615                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.235                           
REMARK   3   R VALUE            (WORKING SET) : 0.233                           
REMARK   3   FREE R VALUE                     : 0.277                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.030                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 4049                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.95                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.00                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 4421                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 67.21                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.3170                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 236                          
REMARK   3   BIN FREE R VALUE                    : 0.3750                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 9206                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 108                                     
REMARK   3   SOLVENT ATOMS            : 726                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 28.17                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 30.90                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 0.35000                                              
REMARK   3    B22 (A**2) : -0.28300                                             
REMARK   3    B33 (A**2) : -0.09300                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : -0.37400                                             
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.257         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.211         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.145         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 5.056         
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.898                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.863                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  9543 ; 0.008 ; 0.020       
REMARK   3   BOND LENGTHS OTHERS               (A):  6440 ; 0.001 ; 0.020       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES): 12965 ; 1.244 ; 1.992       
REMARK   3   BOND ANGLES OTHERS          (DEGREES): 15696 ; 0.874 ; 3.002       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):  1176 ; 6.109 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   436 ;33.325 ;24.289       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1577 ;14.617 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    56 ;15.744 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):  1406 ; 0.067 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A): 10689 ; 0.005 ; 0.021       
REMARK   3   GENERAL PLANES OTHERS             (A):  1939 ; 0.001 ; 0.020       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  2589 ; 0.220 ; 0.200       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):   118 ; 0.246 ; 0.200       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  4658 ; 0.176 ; 0.200       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   246 ; 0.133 ; 0.200       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  9542 ; 1.653 ; 3.536       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  6440 ; 0.382 ; 3.635       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2): 12962 ; 2.622 ; 5.267       
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  3245 ; 4.460 ;10.860       
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  8069 ; 4.795 ;17.523       
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : BABINET MODEL PLUS MASK                              
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.40                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : NULL                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING   
REMARK   3  POSITIONS.                                                          
REMARK   4                                                                      
REMARK   4 3ZLK COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 01-FEB-13.                  
REMARK 100 THE DEPOSITION ID IS D_1290055194.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 28-JUL-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6                                  
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : ROTATING ANODE                     
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-007 HF             
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : MIRRORS                            
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU SATURN 944                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XSCALE                             
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 81490                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.950                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 37.230                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 85.5                               
REMARK 200  DATA REDUNDANCY                : 2.500                              
REMARK 200  R MERGE                    (I) : 0.04000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 14.7000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.95                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.00                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 69.0                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.30                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.47000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: MOLREP                                                
REMARK 200 STARTING MODEL: PDB ENTRY 4ARW                                       
REMARK 200                                                                      
REMARK 200 REMARK: NONE                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 52.30                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.58                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 4% PEG6000, 0.1M MES PH6, 0.05M MGCL2,   
REMARK 280  0.1M NABR, 1% B-ME                                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       32.15000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       77.50000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       32.15000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       77.50000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 13810 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 44730 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -99.8 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000       64.30000            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 13620 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 42690 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -105.4 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000       59.53741            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000      134.36562            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A2092  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH C2076  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH C2079  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH D2055  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH D2107  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     HIS A    -4                                                      
REMARK 465     GLY A    -3                                                      
REMARK 465     SER A    -2                                                      
REMARK 465     MET A    -1                                                      
REMARK 465     ALA A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     HIS B    -9                                                      
REMARK 465     HIS B    -8                                                      
REMARK 465     HIS B    -7                                                      
REMARK 465     HIS B    -6                                                      
REMARK 465     HIS B    -5                                                      
REMARK 465     HIS B    -4                                                      
REMARK 465     GLY B    -3                                                      
REMARK 465     SER B    -2                                                      
REMARK 465     MET B    -1                                                      
REMARK 465     ALA B     0                                                      
REMARK 465     HIS C    -9                                                      
REMARK 465     HIS C    -8                                                      
REMARK 465     HIS C    -7                                                      
REMARK 465     HIS C    -6                                                      
REMARK 465     HIS C    -5                                                      
REMARK 465     HIS C    -4                                                      
REMARK 465     GLY C    -3                                                      
REMARK 465     SER C    -2                                                      
REMARK 465     MET C    -1                                                      
REMARK 465     ALA C     0                                                      
REMARK 465     MET C     1                                                      
REMARK 465     HIS D    -9                                                      
REMARK 465     HIS D    -8                                                      
REMARK 465     HIS D    -7                                                      
REMARK 465     HIS D    -6                                                      
REMARK 465     HIS D    -5                                                      
REMARK 465     HIS D    -4                                                      
REMARK 465     GLY D    -3                                                      
REMARK 465     SER D    -2                                                      
REMARK 465     MET D    -1                                                      
REMARK 465     ALA D     0                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    SER A  12       34.26   -157.32                                   
REMARK 500    TYR A  31      -95.83     59.64                                   
REMARK 500    ARG A 128       97.30    -68.22                                   
REMARK 500    TYR B  31     -100.15     54.10                                   
REMARK 500    TYR C  31      -86.83     59.39                                   
REMARK 500    ALA D   9       67.92   -103.10                                   
REMARK 500    TYR D  31      -90.69     57.59                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH A2028        DISTANCE =  5.92 ANGSTROMS                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE Y46 A 400                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE Y46 B 400                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE Y46 C 400                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE Y46 D 400                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CL A 1294                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CL C 1294                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CL B 1294                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CL D 1294                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 3ZLL   RELATED DB: PDB                                   
REMARK 900 PSEUDOMONAS AERUGINOSA RMLA IN COMPLEX WITH ALLOSTERIC INHIBITOR     
DBREF  3ZLK A    1   293  UNP    G3XCK4   G3XCK4_PSEAI     1    293             
DBREF  3ZLK B    1   293  UNP    G3XCK4   G3XCK4_PSEAI     1    293             
DBREF  3ZLK C    1   293  UNP    G3XCK4   G3XCK4_PSEAI     1    293             
DBREF  3ZLK D    1   293  UNP    G3XCK4   G3XCK4_PSEAI     1    293             
SEQADV 3ZLK HIS A   -9  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS A   -8  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS A   -7  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS A   -6  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS A   -5  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS A   -4  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK GLY A   -3  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK SER A   -2  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK MET A   -1  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK ALA A    0  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS B   -9  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS B   -8  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS B   -7  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS B   -6  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS B   -5  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS B   -4  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK GLY B   -3  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK SER B   -2  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK MET B   -1  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK ALA B    0  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS C   -9  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS C   -8  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS C   -7  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS C   -6  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS C   -5  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS C   -4  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK GLY C   -3  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK SER C   -2  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK MET C   -1  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK ALA C    0  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS D   -9  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS D   -8  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS D   -7  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS D   -6  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS D   -5  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK HIS D   -4  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK GLY D   -3  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK SER D   -2  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK MET D   -1  UNP  G3XCK4              EXPRESSION TAG                 
SEQADV 3ZLK ALA D    0  UNP  G3XCK4              EXPRESSION TAG                 
SEQRES   1 A  303  HIS HIS HIS HIS HIS HIS GLY SER MET ALA MET LYS ARG          
SEQRES   2 A  303  LYS GLY ILE ILE LEU ALA GLY GLY SER GLY THR ARG LEU          
SEQRES   3 A  303  HIS PRO ALA THR LEU ALA ILE SER LYS GLN LEU LEU PRO          
SEQRES   4 A  303  VAL TYR ASP LYS PRO MET ILE TYR TYR PRO LEU SER THR          
SEQRES   5 A  303  LEU MET LEU ALA GLY ILE ARG GLU ILE LEU ILE ILE SER          
SEQRES   6 A  303  THR PRO GLN ASP THR PRO ARG PHE GLN GLN LEU LEU GLY          
SEQRES   7 A  303  ASP GLY SER ASN TRP GLY LEU ASP LEU GLN TYR ALA VAL          
SEQRES   8 A  303  GLN PRO SER PRO ASP GLY LEU ALA GLN ALA PHE LEU ILE          
SEQRES   9 A  303  GLY GLU SER PHE ILE GLY ASN ASP LEU SER ALA LEU VAL          
SEQRES  10 A  303  LEU GLY ASP ASN LEU TYR TYR GLY HIS ASP PHE HIS GLU          
SEQRES  11 A  303  LEU LEU GLY SER ALA SER GLN ARG GLN THR GLY ALA SER          
SEQRES  12 A  303  VAL PHE ALA TYR HIS VAL LEU ASP PRO GLU ARG TYR GLY          
SEQRES  13 A  303  VAL VAL GLU PHE ASP GLN GLY GLY LYS ALA ILE SER LEU          
SEQRES  14 A  303  GLU GLU LYS PRO LEU GLU PRO LYS SER ASN TYR ALA VAL          
SEQRES  15 A  303  THR GLY LEU TYR PHE TYR ASP GLN GLN VAL VAL ASP ILE          
SEQRES  16 A  303  ALA ARG ASP LEU LYS PRO SER PRO ARG GLY GLU LEU GLU          
SEQRES  17 A  303  ILE THR ASP VAL ASN ARG ALA TYR LEU GLU ARG GLY GLN          
SEQRES  18 A  303  LEU SER VAL GLU ILE MET GLY ARG GLY TYR ALA TRP LEU          
SEQRES  19 A  303  ASP THR GLY THR HIS ASP SER LEU LEU GLU ALA GLY GLN          
SEQRES  20 A  303  PHE ILE ALA THR LEU GLU ASN ARG GLN GLY LEU LYS VAL          
SEQRES  21 A  303  ALA CYS PRO GLU GLU ILE ALA TYR ARG GLN LYS TRP ILE          
SEQRES  22 A  303  ASP ALA ALA GLN LEU GLU LYS LEU ALA ALA PRO LEU ALA          
SEQRES  23 A  303  LYS ASN GLY TYR GLY GLN TYR LEU LYS ARG LEU LEU THR          
SEQRES  24 A  303  GLU THR VAL TYR                                              
SEQRES   1 B  303  HIS HIS HIS HIS HIS HIS GLY SER MET ALA MET LYS ARG          
SEQRES   2 B  303  LYS GLY ILE ILE LEU ALA GLY GLY SER GLY THR ARG LEU          
SEQRES   3 B  303  HIS PRO ALA THR LEU ALA ILE SER LYS GLN LEU LEU PRO          
SEQRES   4 B  303  VAL TYR ASP LYS PRO MET ILE TYR TYR PRO LEU SER THR          
SEQRES   5 B  303  LEU MET LEU ALA GLY ILE ARG GLU ILE LEU ILE ILE SER          
SEQRES   6 B  303  THR PRO GLN ASP THR PRO ARG PHE GLN GLN LEU LEU GLY          
SEQRES   7 B  303  ASP GLY SER ASN TRP GLY LEU ASP LEU GLN TYR ALA VAL          
SEQRES   8 B  303  GLN PRO SER PRO ASP GLY LEU ALA GLN ALA PHE LEU ILE          
SEQRES   9 B  303  GLY GLU SER PHE ILE GLY ASN ASP LEU SER ALA LEU VAL          
SEQRES  10 B  303  LEU GLY ASP ASN LEU TYR TYR GLY HIS ASP PHE HIS GLU          
SEQRES  11 B  303  LEU LEU GLY SER ALA SER GLN ARG GLN THR GLY ALA SER          
SEQRES  12 B  303  VAL PHE ALA TYR HIS VAL LEU ASP PRO GLU ARG TYR GLY          
SEQRES  13 B  303  VAL VAL GLU PHE ASP GLN GLY GLY LYS ALA ILE SER LEU          
SEQRES  14 B  303  GLU GLU LYS PRO LEU GLU PRO LYS SER ASN TYR ALA VAL          
SEQRES  15 B  303  THR GLY LEU TYR PHE TYR ASP GLN GLN VAL VAL ASP ILE          
SEQRES  16 B  303  ALA ARG ASP LEU LYS PRO SER PRO ARG GLY GLU LEU GLU          
SEQRES  17 B  303  ILE THR ASP VAL ASN ARG ALA TYR LEU GLU ARG GLY GLN          
SEQRES  18 B  303  LEU SER VAL GLU ILE MET GLY ARG GLY TYR ALA TRP LEU          
SEQRES  19 B  303  ASP THR GLY THR HIS ASP SER LEU LEU GLU ALA GLY GLN          
SEQRES  20 B  303  PHE ILE ALA THR LEU GLU ASN ARG GLN GLY LEU LYS VAL          
SEQRES  21 B  303  ALA CYS PRO GLU GLU ILE ALA TYR ARG GLN LYS TRP ILE          
SEQRES  22 B  303  ASP ALA ALA GLN LEU GLU LYS LEU ALA ALA PRO LEU ALA          
SEQRES  23 B  303  LYS ASN GLY TYR GLY GLN TYR LEU LYS ARG LEU LEU THR          
SEQRES  24 B  303  GLU THR VAL TYR                                              
SEQRES   1 C  303  HIS HIS HIS HIS HIS HIS GLY SER MET ALA MET LYS ARG          
SEQRES   2 C  303  LYS GLY ILE ILE LEU ALA GLY GLY SER GLY THR ARG LEU          
SEQRES   3 C  303  HIS PRO ALA THR LEU ALA ILE SER LYS GLN LEU LEU PRO          
SEQRES   4 C  303  VAL TYR ASP LYS PRO MET ILE TYR TYR PRO LEU SER THR          
SEQRES   5 C  303  LEU MET LEU ALA GLY ILE ARG GLU ILE LEU ILE ILE SER          
SEQRES   6 C  303  THR PRO GLN ASP THR PRO ARG PHE GLN GLN LEU LEU GLY          
SEQRES   7 C  303  ASP GLY SER ASN TRP GLY LEU ASP LEU GLN TYR ALA VAL          
SEQRES   8 C  303  GLN PRO SER PRO ASP GLY LEU ALA GLN ALA PHE LEU ILE          
SEQRES   9 C  303  GLY GLU SER PHE ILE GLY ASN ASP LEU SER ALA LEU VAL          
SEQRES  10 C  303  LEU GLY ASP ASN LEU TYR TYR GLY HIS ASP PHE HIS GLU          
SEQRES  11 C  303  LEU LEU GLY SER ALA SER GLN ARG GLN THR GLY ALA SER          
SEQRES  12 C  303  VAL PHE ALA TYR HIS VAL LEU ASP PRO GLU ARG TYR GLY          
SEQRES  13 C  303  VAL VAL GLU PHE ASP GLN GLY GLY LYS ALA ILE SER LEU          
SEQRES  14 C  303  GLU GLU LYS PRO LEU GLU PRO LYS SER ASN TYR ALA VAL          
SEQRES  15 C  303  THR GLY LEU TYR PHE TYR ASP GLN GLN VAL VAL ASP ILE          
SEQRES  16 C  303  ALA ARG ASP LEU LYS PRO SER PRO ARG GLY GLU LEU GLU          
SEQRES  17 C  303  ILE THR ASP VAL ASN ARG ALA TYR LEU GLU ARG GLY GLN          
SEQRES  18 C  303  LEU SER VAL GLU ILE MET GLY ARG GLY TYR ALA TRP LEU          
SEQRES  19 C  303  ASP THR GLY THR HIS ASP SER LEU LEU GLU ALA GLY GLN          
SEQRES  20 C  303  PHE ILE ALA THR LEU GLU ASN ARG GLN GLY LEU LYS VAL          
SEQRES  21 C  303  ALA CYS PRO GLU GLU ILE ALA TYR ARG GLN LYS TRP ILE          
SEQRES  22 C  303  ASP ALA ALA GLN LEU GLU LYS LEU ALA ALA PRO LEU ALA          
SEQRES  23 C  303  LYS ASN GLY TYR GLY GLN TYR LEU LYS ARG LEU LEU THR          
SEQRES  24 C  303  GLU THR VAL TYR                                              
SEQRES   1 D  303  HIS HIS HIS HIS HIS HIS GLY SER MET ALA MET LYS ARG          
SEQRES   2 D  303  LYS GLY ILE ILE LEU ALA GLY GLY SER GLY THR ARG LEU          
SEQRES   3 D  303  HIS PRO ALA THR LEU ALA ILE SER LYS GLN LEU LEU PRO          
SEQRES   4 D  303  VAL TYR ASP LYS PRO MET ILE TYR TYR PRO LEU SER THR          
SEQRES   5 D  303  LEU MET LEU ALA GLY ILE ARG GLU ILE LEU ILE ILE SER          
SEQRES   6 D  303  THR PRO GLN ASP THR PRO ARG PHE GLN GLN LEU LEU GLY          
SEQRES   7 D  303  ASP GLY SER ASN TRP GLY LEU ASP LEU GLN TYR ALA VAL          
SEQRES   8 D  303  GLN PRO SER PRO ASP GLY LEU ALA GLN ALA PHE LEU ILE          
SEQRES   9 D  303  GLY GLU SER PHE ILE GLY ASN ASP LEU SER ALA LEU VAL          
SEQRES  10 D  303  LEU GLY ASP ASN LEU TYR TYR GLY HIS ASP PHE HIS GLU          
SEQRES  11 D  303  LEU LEU GLY SER ALA SER GLN ARG GLN THR GLY ALA SER          
SEQRES  12 D  303  VAL PHE ALA TYR HIS VAL LEU ASP PRO GLU ARG TYR GLY          
SEQRES  13 D  303  VAL VAL GLU PHE ASP GLN GLY GLY LYS ALA ILE SER LEU          
SEQRES  14 D  303  GLU GLU LYS PRO LEU GLU PRO LYS SER ASN TYR ALA VAL          
SEQRES  15 D  303  THR GLY LEU TYR PHE TYR ASP GLN GLN VAL VAL ASP ILE          
SEQRES  16 D  303  ALA ARG ASP LEU LYS PRO SER PRO ARG GLY GLU LEU GLU          
SEQRES  17 D  303  ILE THR ASP VAL ASN ARG ALA TYR LEU GLU ARG GLY GLN          
SEQRES  18 D  303  LEU SER VAL GLU ILE MET GLY ARG GLY TYR ALA TRP LEU          
SEQRES  19 D  303  ASP THR GLY THR HIS ASP SER LEU LEU GLU ALA GLY GLN          
SEQRES  20 D  303  PHE ILE ALA THR LEU GLU ASN ARG GLN GLY LEU LYS VAL          
SEQRES  21 D  303  ALA CYS PRO GLU GLU ILE ALA TYR ARG GLN LYS TRP ILE          
SEQRES  22 D  303  ASP ALA ALA GLN LEU GLU LYS LEU ALA ALA PRO LEU ALA          
SEQRES  23 D  303  LYS ASN GLY TYR GLY GLN TYR LEU LYS ARG LEU LEU THR          
SEQRES  24 D  303  GLU THR VAL TYR                                              
HET    Y46  A 400      26                                                       
HET     CL  A1294       1                                                       
HET    Y46  B 400      26                                                       
HET     CL  B1294       1                                                       
HET    Y46  C 400      26                                                       
HET     CL  C1294       1                                                       
HET    Y46  D 400      26                                                       
HET     CL  D1294       1                                                       
HETNAM     Y46 N-(6-AMINO-1-BENZYL-2,4-DIOXO-1,2,3,4-                           
HETNAM   2 Y46  TETRAHYDROPYRIMIDIN-5-YL)BENZENESULFONAMIDE                     
HETNAM      CL CHLORIDE ION                                                     
FORMUL   5  Y46    4(C17 H16 N4 O4 S)                                           
FORMUL   6   CL    4(CL 1-)                                                     
FORMUL  13  HOH   *726(H2 O)                                                    
HELIX    1   1 SER A   24  LEU A   27  5                                   4    
HELIX    2   2 ILE A   36  ALA A   46  1                                  11    
HELIX    3   3 ASP A   59  GLY A   68  1                                  10    
HELIX    4   4 GLY A   70  GLY A   74  5                                   5    
HELIX    5   5 GLY A   87  ALA A   89  5                                   3    
HELIX    6   6 GLN A   90  GLY A   95  1                                   6    
HELIX    7   7 GLY A   95  GLY A  100  1                                   6    
HELIX    8   8 ASP A  117  ARG A  128  1                                  12    
HELIX    9   9 ASP A  141  ARG A  144  5                                   4    
HELIX   10  10 GLN A  181  ASP A  188  1                                   8    
HELIX   11  11 GLU A  198  ARG A  209  1                                  12    
HELIX   12  12 THR A  228  GLY A  247  1                                  20    
HELIX   13  13 CYS A  252  GLN A  260  1                                   9    
HELIX   14  14 ASP A  264  ALA A  273  1                                  10    
HELIX   15  15 PRO A  274  ALA A  276  5                                   3    
HELIX   16  16 ASN A  278  LEU A  288  1                                  11    
HELIX   17  17 SER B   24  LEU B   27  5                                   4    
HELIX   18  18 ILE B   36  ALA B   46  1                                  11    
HELIX   19  19 ASP B   59  GLY B   68  1                                  10    
HELIX   20  20 GLY B   70  GLY B   74  5                                   5    
HELIX   21  21 ALA B   89  GLY B   95  1                                   7    
HELIX   22  22 GLY B   95  GLY B  100  1                                   6    
HELIX   23  23 ASP B  117  ARG B  128  1                                  12    
HELIX   24  24 ASP B  141  ARG B  144  5                                   4    
HELIX   25  25 GLN B  181  ARG B  187  1                                   7    
HELIX   26  26 GLU B  198  ARG B  209  1                                  12    
HELIX   27  27 THR B  228  GLY B  247  1                                  20    
HELIX   28  28 CYS B  252  GLN B  260  1                                   9    
HELIX   29  29 ASP B  264  ALA B  273  1                                  10    
HELIX   30  30 ASN B  278  LEU B  287  1                                  10    
HELIX   31  31 PRO C   18  ILE C   23  1                                   6    
HELIX   32  32 SER C   24  LEU C   27  5                                   4    
HELIX   33  33 ILE C   36  ALA C   46  1                                  11    
HELIX   34  34 ASP C   59  GLY C   68  1                                  10    
HELIX   35  35 GLY C   70  GLY C   74  5                                   5    
HELIX   36  36 GLY C   87  ALA C   89  5                                   3    
HELIX   37  37 GLN C   90  GLY C   95  1                                   6    
HELIX   38  38 GLY C   95  GLY C  100  1                                   6    
HELIX   39  39 ASP C  117  ARG C  128  1                                  12    
HELIX   40  40 ASP C  141  ARG C  144  5                                   4    
HELIX   41  41 GLN C  181  ASP C  188  1                                   8    
HELIX   42  42 GLU C  198  ARG C  209  1                                  12    
HELIX   43  43 THR C  228  GLY C  247  1                                  20    
HELIX   44  44 CYS C  252  GLN C  260  1                                   9    
HELIX   45  45 ASP C  264  ALA C  273  1                                  10    
HELIX   46  46 PRO C  274  ALA C  276  5                                   3    
HELIX   47  47 ASN C  278  GLU C  290  1                                  13    
HELIX   48  48 SER D   24  LEU D   27  5                                   4    
HELIX   49  49 ILE D   36  ALA D   46  1                                  11    
HELIX   50  50 ASP D   59  GLY D   68  1                                  10    
HELIX   51  51 GLY D   70  GLY D   74  5                                   5    
HELIX   52  52 ALA D   89  GLY D   95  1                                   7    
HELIX   53  53 GLY D   95  GLY D  100  1                                   6    
HELIX   54  54 ASP D  117  ARG D  128  1                                  12    
HELIX   55  55 ASP D  141  ARG D  144  5                                   4    
HELIX   56  56 GLN D  181  ASP D  188  1                                   8    
HELIX   57  57 GLU D  198  ARG D  209  1                                  12    
HELIX   58  58 THR D  228  GLY D  247  1                                  20    
HELIX   59  59 CYS D  252  GLN D  260  1                                   9    
HELIX   60  60 ASP D  264  ALA D  273  1                                  10    
HELIX   61  61 PRO D  274  ALA D  276  5                                   3    
HELIX   62  62 ASN D  278  LEU D  288  1                                  11    
SHEET    1  AA 5 ASP A  76  VAL A  81  0                                        
SHEET    2  AA 5 GLU A  50  SER A  55  1  O  ILE A  51   N  GLN A  78           
SHEET    3  AA 5 LYS A   4  ALA A   9  1  O  GLY A   5   N  LEU A  52           
SHEET    4  AA 5 SER A 104  LEU A 108  1  O  ALA A 105   N  ILE A   6           
SHEET    5  AA 5 LEU A 175  TYR A 178 -1  O  TYR A 176   N  LEU A 106           
SHEET    1  AB 2 PRO A  29  VAL A  30  0                                        
SHEET    2  AB 2 LYS A  33  PRO A  34 -1  O  LYS A  33   N  VAL A  30           
SHEET    1  AC 2 LEU A 112  TYR A 114  0                                        
SHEET    2  AC 2 ALA A 222  LEU A 224 -1  O  ALA A 222   N  TYR A 114           
SHEET    1  AD 3 TYR A 170  VAL A 172  0                                        
SHEET    2  AD 3 ALA A 132  HIS A 138 -1  O  TYR A 137   N  ALA A 171           
SHEET    3  AD 3 LEU A 212  ILE A 216  1  O  SER A 213   N  VAL A 134           
SHEET    1  AE 2 GLY A 146  PHE A 150  0                                        
SHEET    2  AE 2 ALA A 156  GLU A 161 -1  N  ILE A 157   O  GLU A 149           
SHEET    1  BA 7 ASP B  76  VAL B  81  0                                        
SHEET    2  BA 7 GLU B  50  SER B  55  1  O  ILE B  51   N  GLN B  78           
SHEET    3  BA 7 ARG B   3  ALA B   9  1  O  GLY B   5   N  LEU B  52           
SHEET    4  BA 7 LEU B 103  LEU B 108  1  O  LEU B 103   N  LYS B   4           
SHEET    5  BA 7 TYR B 170  TYR B 178 -1  O  GLY B 174   N  LEU B 108           
SHEET    6  BA 7 ALA B 132  HIS B 138 -1  O  SER B 133   N  PHE B 177           
SHEET    7  BA 7 LEU B 212  ILE B 216  1  O  SER B 213   N  VAL B 134           
SHEET    1  BB 2 PRO B  29  VAL B  30  0                                        
SHEET    2  BB 2 LYS B  33  PRO B  34 -1  O  LYS B  33   N  VAL B  30           
SHEET    1  BC 2 LEU B 112  TYR B 114  0                                        
SHEET    2  BC 2 ALA B 222  LEU B 224 -1  O  ALA B 222   N  TYR B 114           
SHEET    1  BD 2 GLY B 146  PHE B 150  0                                        
SHEET    2  BD 2 ALA B 156  GLU B 161 -1  N  ILE B 157   O  GLU B 149           
SHEET    1  CA 5 ASP C  76  VAL C  81  0                                        
SHEET    2  CA 5 GLU C  50  SER C  55  1  O  ILE C  51   N  GLN C  78           
SHEET    3  CA 5 ARG C   3  LEU C   8  1  O  GLY C   5   N  LEU C  52           
SHEET    4  CA 5 LEU C 103  LEU C 108  1  O  LEU C 103   N  LYS C   4           
SHEET    5  CA 5 LEU C 175  TYR C 178 -1  O  TYR C 176   N  LEU C 106           
SHEET    1  CB 2 PRO C  29  VAL C  30  0                                        
SHEET    2  CB 2 LYS C  33  PRO C  34 -1  O  LYS C  33   N  VAL C  30           
SHEET    1  CC 2 LEU C 112  TYR C 114  0                                        
SHEET    2  CC 2 ALA C 222  LEU C 224 -1  O  ALA C 222   N  TYR C 114           
SHEET    1  CD 3 TYR C 170  VAL C 172  0                                        
SHEET    2  CD 3 ALA C 132  HIS C 138 -1  O  TYR C 137   N  ALA C 171           
SHEET    3  CD 3 LEU C 212  ILE C 216  1  O  SER C 213   N  VAL C 134           
SHEET    1  CE 2 GLY C 146  PHE C 150  0                                        
SHEET    2  CE 2 ALA C 156  GLU C 161 -1  N  ILE C 157   O  GLU C 149           
SHEET    1  DA 7 ASP D  76  VAL D  81  0                                        
SHEET    2  DA 7 GLU D  50  SER D  55  1  O  ILE D  51   N  GLN D  78           
SHEET    3  DA 7 ARG D   3  ALA D   9  1  O  GLY D   5   N  LEU D  52           
SHEET    4  DA 7 LEU D 103  LEU D 108  1  O  LEU D 103   N  LYS D   4           
SHEET    5  DA 7 TYR D 170  TYR D 178 -1  O  GLY D 174   N  LEU D 108           
SHEET    6  DA 7 ALA D 132  HIS D 138 -1  O  SER D 133   N  PHE D 177           
SHEET    7  DA 7 LEU D 212  ILE D 216  1  O  SER D 213   N  VAL D 134           
SHEET    1  DB 2 PRO D  29  VAL D  30  0                                        
SHEET    2  DB 2 LYS D  33  PRO D  34 -1  O  LYS D  33   N  VAL D  30           
SHEET    1  DC 2 ASN D 111  TYR D 114  0                                        
SHEET    2  DC 2 ALA D 222  ASP D 225 -1  O  ALA D 222   N  TYR D 114           
SHEET    1  DD 2 GLY D 146  PHE D 150  0                                        
SHEET    2  DD 2 ALA D 156  GLU D 161 -1  N  ILE D 157   O  GLU D 149           
CISPEP   1 HIS A   17    PRO A   18          0        15.10                     
CISPEP   2 HIS B   17    PRO B   18          0         6.77                     
CISPEP   3 HIS C   17    PRO C   18          0         6.25                     
CISPEP   4 HIS D   17    PRO D   18          0         6.80                     
SITE     1 AC1 16 SER A  41  LEU A  45  TYR A 113  TYR A 114                    
SITE     2 AC1 16 GLY A 115  PHE A 118  ARG A 219  LYS A 249                    
SITE     3 AC1 16 VAL A 250  ALA A 251  GLU A 255  ILE A 256                    
SITE     4 AC1 16 HOH A2091  HOH A2094  HOH A2180  HOH A2208                    
SITE     1 AC2 15 SER B  41  LEU B  45  TYR B 113  TYR B 114                    
SITE     2 AC2 15 GLY B 115  PHE B 118  ARG B 219  LYS B 249                    
SITE     3 AC2 15 VAL B 250  ALA B 251  GLU B 255  HOH B2080                    
SITE     4 AC2 15 HOH B2082  HOH B2166  HOH B2189                               
SITE     1 AC3 16 SER C  41  LEU C  45  TYR C 113  TYR C 114                    
SITE     2 AC3 16 GLY C 115  PHE C 118  ARG C 219  LYS C 249                    
SITE     3 AC3 16 VAL C 250  ALA C 251  GLU C 255  HOH C2028                    
SITE     4 AC3 16 HOH C2075  HOH C2159  HOH C2189  HOH C2190                    
SITE     1 AC4 15 SER D  41  LEU D  45  TYR D 113  TYR D 114                    
SITE     2 AC4 15 GLY D 115  PHE D 118  ARG D 219  LYS D 249                    
SITE     3 AC4 15 VAL D 250  ALA D 251  GLU D 255  HOH D2054                    
SITE     4 AC4 15 HOH D2057  HOH D2112  HOH D2139                               
SITE     1 AC5  3 HIS A 116  ARG A 219  GLY A 220                               
SITE     1 AC6  4 HIS C 116  ARG C 219  GLY C 220  HOH C2078                    
SITE     1 AC7  4 HIS B 116  GLY B 218  ARG B 219  GLY B 220                    
SITE     1 AC8  2 HIS D 116  GLY D 220                                          
CRYST1   64.300  155.000  134.450  90.00  92.03  90.00 C 1 2 1      16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.015552  0.000000  0.000551        0.00000                         
SCALE2      0.000000  0.006452  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.007442        0.00000                         
MTRIX1   1 -0.937500  0.011010 -0.347900       62.63000    1                    
MTRIX2   1 -0.006215 -0.999900 -0.014910      -36.30000    1                    
MTRIX3   1 -0.348000 -0.011820  0.937400       10.75000    1                    
MTRIX1   2  0.998900 -0.005255 -0.047350        5.53500    1                    
MTRIX2   2 -0.004625 -0.999900  0.013390      -17.53000    1                    
MTRIX3   2 -0.047420 -0.013160 -0.998800       68.24000    1                    
MTRIX1   3 -0.953400  0.008598  0.301600       40.13000    1                    
MTRIX2   3  0.011610  0.999900  0.008208      -20.66000    1                    
MTRIX3   3 -0.301500  0.011330 -0.953400       72.68000    1                    
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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