GenomeNet

Database: PDB
Entry: 4AJY
LinkDB: 4AJY
Original site: 4AJY 
HEADER    TRANSCRIPTION                           21-FEB-12   4AJY              
TITLE     VON HIPPEL-LINDAU PROTEIN-ELONGINB-ELONGINC COMPLEX, BOUND TO HIF1-   
TITLE    2 ALPHA PEPTIDE                                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2;           
COMPND   3 CHAIN: B;                                                            
COMPND   4 SYNONYM: ELONGIN 18 KDA SUBUNIT, ELONGIN-B, ELOB, RNA POLYMERASE II  
COMPND   5  TRANSCRIPTION FACTOR SIII SUBUNIT B, SIII P18;                      
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 1;           
COMPND   9 CHAIN: C;                                                            
COMPND  10 FRAGMENT: 17-112;                                                    
COMPND  11 SYNONYM: ELONGIN 15 KDA SUBUNIT, ELONGIN-C, ELOC, RNA POLYMERASE II  
COMPND  12  TRANSCRIPTION FACTOR SIII SUBUNIT C, SIII P15;                      
COMPND  13 ENGINEERED: YES;                                                     
COMPND  14 OTHER_DETAILS: EXTRA MET AT N-TERMINUS DUE TO CLONING;               
COMPND  15 MOL_ID: 3;                                                           
COMPND  16 MOLECULE: HYPOXIA-INDUCIBLE FACTOR 1-ALPHA;                          
COMPND  17 CHAIN: H;                                                            
COMPND  18 FRAGMENT: RESIDUES 559-577;                                          
COMPND  19 SYNONYM: HIF-1-ALPHA, HIF1-ALPHA, ARNT-INTERACTING PROTEIN, BASIC-   
COMPND  20  HELIX-LOOP-HELIX-PAS PROTEIN MOP1, CLASS E BASIC HELIX-LOOP-HELIX   
COMPND  21  PROTEIN 78, BHLHE78, MEMBER OF PAS PROTEIN 1, PAS                   
COMPND  22  DOMAIN-CONTAINING PROTEIN 8;                                        
COMPND  23 MUTATION: YES;                                                       
COMPND  24 MOL_ID: 4;                                                           
COMPND  25 MOLECULE: VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR;                
COMPND  26 CHAIN: V;                                                            
COMPND  27 FRAGMENT: RESIDUES 54-213;                                           
COMPND  28 SYNONYM: PROTEIN G7, PVHL, PVHL54-213;                               
COMPND  29 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_VECTOR: PCDF_DUET;                                 
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_COMMON: HUMAN;                                              
SOURCE  13 ORGANISM_TAXID: 9606;                                                
SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  16 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  17 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  18 EXPRESSION_SYSTEM_VECTOR: PCDF_DUET;                                 
SOURCE  19 MOL_ID: 3;                                                           
SOURCE  20 SYNTHETIC: YES;                                                      
SOURCE  21 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  22 ORGANISM_COMMON: HUMAN SOURCE 19;                                    
SOURCE  23 ORGANISM_TAXID: 9606;                                                
SOURCE  24 MOL_ID: 4;                                                           
SOURCE  25 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  26 ORGANISM_COMMON: HUMAN;                                              
SOURCE  27 ORGANISM_TAXID: 9606;                                                
SOURCE  28 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  29 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  30 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  31 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  32 EXPRESSION_SYSTEM_VECTOR: PET28A                                     
KEYWDS    E3 UBIQUITIN LIGASE, TRANSCRIPTION FACTOR, HYPOXIC SIGNALING,         
KEYWDS   2 TRANSCRIPTION                                                        
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    I.VAN MOLLE,A.THOMANN,D.L.BUCKLEY,E.C.SO,S.LANG,C.M.CREWS,A.CIULLI    
REVDAT   1   14-NOV-12 4AJY    0                                                
JRNL        AUTH   I.VAN MOLLE,A.THOMANN,D.L.BUCKLEY,E.C.SO,S.LANG,C.M.CREWS,   
JRNL        AUTH 2 A.CIULLI                                                     
JRNL        TITL   DISSECTING FRAGMENT-BASED LEAD DISCOVERY AT THE VON HIPPEL-  
JRNL        TITL 2 LINDAU PROTEIN:HYPOXIA INDUCIBLE FACTOR 1ALPHA PROTEIN-      
JRNL        TITL 3 PROTEIN INTERFACE.                                           
JRNL        REF    CHEM.BIOL.                    V.  19  1300 2012              
JRNL        REFN                   ISSN 1879-1301                               
JRNL        PMID   23102223                                                     
JRNL        DOI    10.1016/J.CHEMBIOL.2012.08.015                               
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.73 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.6.0117                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.73                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 42.50                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NONE                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.69                          
REMARK   3   NUMBER OF REFLECTIONS             : 43989                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.19241                         
REMARK   3   R VALUE            (WORKING SET) : 0.19032                         
REMARK   3   FREE R VALUE                     : 0.23241                         
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.0                             
REMARK   3   FREE R VALUE TEST SET COUNT      : 2316                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.733                        
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.778                        
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 2803                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 97.19                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.257                        
REMARK   3   BIN FREE R VALUE SET COUNT          : 142                          
REMARK   3   BIN FREE R VALUE                    : 0.334                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2812                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 6                                       
REMARK   3   SOLVENT ATOMS            : 242                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 24.567                         
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 0.00                                                 
REMARK   3    B22 (A**2) : 0.00                                                 
REMARK   3    B33 (A**2) : 0.00                                                 
REMARK   3    B12 (A**2) : 0.00                                                 
REMARK   3    B13 (A**2) : 0.00                                                 
REMARK   3    B23 (A**2) : 0.00                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.104         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.108         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.068         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 2.097         
REMARK   3                                                                      
REMARK   3  CORRELATION COEFFICIENTS.                                           
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.953                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.930                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  2884 ; 0.022 ; 0.020       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  3924 ; 2.178 ; 1.988       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   355 ; 6.301 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   127 ;36.635 ;23.465       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   473 ;13.952 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    22 ;21.341 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   450 ; 0.168 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  2184 ; 0.014 ; 0.022       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ANISOTROPIC THERMAL FACTOR RESTRAINTS.   COUNT   RMS    WEIGHT      
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : MASK                                                 
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.20                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING   
REMARK   3  POSITIONS. HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT. U      
REMARK   3  VALUES REFINED INDIVIDUALLY                                         
REMARK   4                                                                      
REMARK   4 4AJY COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 21-FEB-12.                  
REMARK 100 THE PDBE ID CODE IS EBI-51389.                                       
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 22-APR-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SOLEIL                             
REMARK 200  BEAMLINE                       : PROXIMA 1                          
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.98011                            
REMARK 200  MONOCHROMATOR                  : CHANNEL CUT CRYOGENICALLY COOLED   
REMARK 200                                   MONOCHROMATOR CRYSTAL              
REMARK 200  OPTICS                         : KIRKPATRICK-BAEZ PAIR OF           
REMARK 200                                   BI-MORPH MIRRORS                   
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD(QUANTUM 315R)                  
REMARK 200  DETECTOR MANUFACTURER          : ADSC                               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS                                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 46307                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.73                               
REMARK 200  RESOLUTION RANGE LOW       (A) : 45.00                              
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 3.0                                
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY                : 7.4                                
REMARK 200  R MERGE                    (I) : 0.06                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 22.19                              
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.73                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.84                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.8                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.1                                
REMARK 200  R MERGE FOR SHELL          (I) : 0.54                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 3.09                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1LM8                                       
REMARK 200                                                                      
REMARK 200 REMARK: NONE                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 50.36                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.5                      
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M K PHOSPHATE PH 6.6 0.2 M           
REMARK 280  (NH4)2SO4 20% PEG MME 5000 5 MM DTT                                 
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+3/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+3/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      122.01900            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       29.61600            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       29.61600            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000      183.02850            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       29.61600            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       29.61600            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       61.00950            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       29.61600            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       29.61600            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000      183.02850            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       29.61600            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       29.61600            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       61.00950            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000      122.01900            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 6580 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 17050 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -48.9 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: V, B, C, H                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLN B   106                                                      
REMARK 465     ASP B   107                                                      
REMARK 465     SER B   108                                                      
REMARK 465     GLY B   109                                                      
REMARK 465     SER B   110                                                      
REMARK 465     SER B   111                                                      
REMARK 465     ALA B   112                                                      
REMARK 465     ASN B   113                                                      
REMARK 465     GLU B   114                                                      
REMARK 465     GLN B   115                                                      
REMARK 465     ALA B   116                                                      
REMARK 465     VAL B   117                                                      
REMARK 465     GLN B   118                                                      
REMARK 465     MET C    16                                                      
REMARK 465     GLY C    50                                                      
REMARK 465     GLN C    51                                                      
REMARK 465     PHE C    52                                                      
REMARK 465     ALA C    53                                                      
REMARK 465     GLU C    54                                                      
REMARK 465     ASN C    55                                                      
REMARK 465     GLU C    56                                                      
REMARK 465     THR C    57                                                      
REMARK 465     ASP H   559                                                      
REMARK 465     ARG H   575                                                      
REMARK 465     SER H   576                                                      
REMARK 465     PHE H   577                                                      
REMARK 465     GLY V    51                                                      
REMARK 465     SER V    52                                                      
REMARK 465     HIS V    53                                                      
REMARK 465     MET V    54                                                      
REMARK 465     GLU V    55                                                      
REMARK 465     ALA V    56                                                      
REMARK 465     GLY V    57                                                      
REMARK 465     ARG V    58                                                      
REMARK 465     PRO V    59                                                      
REMARK 465     HIS V   208                                                      
REMARK 465     GLN V   209                                                      
REMARK 465     ARG V   210                                                      
REMARK 465     MET V   211                                                      
REMARK 465     GLY V   212                                                      
REMARK 465     ASP V   213                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG B  80    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP B  82    CG   OD1  OD2                                       
REMARK 470     ASP B  83    CG   OD1  OD2                                       
REMARK 470     ARG C  63    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU H 560    CG   CD   OE1  OE2                                  
REMARK 470     ARG V  69    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLN V  73    CG   CD   OE1  NE2                                  
REMARK 470     GLU V  94    CG   CD   OE1  OE2                                  
REMARK 470     VAL V 142    CG1  CG2                                            
REMARK 470     ASP V 143    CG   OD1  OD2                                       
REMARK 470     GLN V 145    CG   CD   OE1  NE2                                  
REMARK 470     ILE V 147    CG1  CG2  CD1                                       
REMARK 470     LYS V 171    CG   CD   CE   NZ                                   
REMARK 470     ARG V 182    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU V 199    CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    GLU C 102   CD    GLU C 102   OE1     0.073                       
REMARK 500    TRP V 117   CD2   TRP V 117   CE2     0.074                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    ARG B  29   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500    PRO C  49   C   -  N   -  CA  ANGL. DEV. =   9.2 DEGREES          
REMARK 500    ARG V 113   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500    ARG V 120   NE  -  CZ  -  NH1 ANGL. DEV. =   3.0 DEGREES          
REMARK 500    ARG V 120   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES          
REMARK 500    ARG V 205   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.2 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    HIS B  10     -120.36     56.08                                   
REMARK 500    ASP B  47     -122.01     53.79                                   
REMARK 500    ALA B  71       66.39   -151.54                                   
REMARK 500    ASP B  83       12.42   -151.46                                   
REMARK 500    ARG V  69       17.76     55.18                                   
REMARK 500    ARG V  79       46.56    -99.86                                   
REMARK 500    SER V 111     -157.48   -123.12                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL V1208                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1D7G   RELATED DB: PDB                                   
REMARK 900  A MODEL FOR THE COMPLEX BETWEEN THE HYPOXIA-INDUCIBLE               
REMARK 900   FACTOR-1 (HIF-1) AND ITS CONSENSUS DEOXYRIBONUCLEIC                
REMARK 900  ACID SEQUENCE                                                       
REMARK 900 RELATED ID: 1H2K   RELATED DB: PDB                                   
REMARK 900  FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH HIF-1                 
REMARK 900   ALPHA FRAGMENT PEPTIDE                                             
REMARK 900 RELATED ID: 1H2L   RELATED DB: PDB                                   
REMARK 900  FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH HIF-1                 
REMARK 900   ALPHA FRAGMENT PEPTIDE                                             
REMARK 900 RELATED ID: 1H2M   RELATED DB: PDB                                   
REMARK 900  FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH HIF-1                 
REMARK 900   ALPHA FRAGMENT PEPTIDE                                             
REMARK 900 RELATED ID: 1L3E   RELATED DB: PDB                                   
REMARK 900  NMR STRUCTURES OF THE HIF-1ALPHA CTAD/P300 CH1                      
REMARK 900  COMPLEX                                                             
REMARK 900 RELATED ID: 1L8C   RELATED DB: PDB                                   
REMARK 900  STRUCTURAL BASIS FOR HIF-1ALPHA/CBP RECOGNITION IN                  
REMARK 900  THECELLULAR HYPOXIC RESPONSE                                        
REMARK 900 RELATED ID: 1LM8   RELATED DB: PDB                                   
REMARK 900  STRUCTURE OF A HIF-1A-PVHL-ELONGINB-ELONGINC COMPLEX                
REMARK 900 RELATED ID: 1LQB   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF A HYDROXYLATED HIF-1 ALPHA                     
REMARK 900  PEPTIDEBOUND TO THE PVHL/ELONGIN-C/ELONGIN-B COMPLEX                
REMARK 900 RELATED ID: 1VCB   RELATED DB: PDB                                   
REMARK 900  THE VHL-ELONGINC-ELONGINB STRUCTURE                                 
REMARK 900 RELATED ID: 2C9W   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF SOCS-2 IN COMPLEX WITH ELONGIN-                
REMARK 900  B AND ELONGIN-C AT 1.9A RESOLUTION                                  
REMARK 900 RELATED ID: 2IZV   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF SOCS-4 IN COMPLEX WITH ELONGIN-                
REMARK 900  B AND ELONGIN-C AT 2.55A RESOLUTION                                 
REMARK 900 RELATED ID: 2XAI   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF ANKYRIN REPEAT AND SOCS BOX-                   
REMARK 900  CONTAINING PROTEIN 9 (ASB9) IN COMPLEX WITH ELONGINB                
REMARK 900  AND ELONGINC                                                        
REMARK 900 RELATED ID: 3ZRC   RELATED DB: PDB                                   
REMARK 900  PVHL54-213-ELOB-ELOC COMPLEX (4R)-4-HYDROXY-1-[(3-                  
REMARK 900  METHYLISOXAZOL-5-YL)ACETYL]-N-[4-(1,3-OXAZOL-5-YL                   
REMARK 900  )BENZYL]-L-PROLINAMIDE BOUND                                        
REMARK 900 RELATED ID: 3ZRF   RELATED DB: PDB                                   
REMARK 900  PVHL54-213-ELOB-ELOC COMPLEX_APO                                    
REMARK 900 RELATED ID: 3ZTC   RELATED DB: PDB                                   
REMARK 900  PVHL54-213-ELOB-ELOC COMPLEX _ (2S,4R)-N-((1,1'-                    
REMARK 900  BIPHENYL)-4-YLMETHYL)-4-HYDROXY-1-(2-(3-METHYLISOXAZOL              
REMARK 900  -5-YL)ACETYL)PYRROLIDINE-2-CARBOXAMIDE                              
REMARK 900 RELATED ID: 3ZTD   RELATED DB: PDB                                   
REMARK 900  PVHL54-213-ELOB-ELOC COMPLEX _ METHYL 4-(((2S,4R)-                  
REMARK 900  4-HYDROXY-1-(2-(3-METHYLISOXAZOL-5-YL)ACETYL)                       
REMARK 900  PYRROLIDINE-2-CARBOXAMIDO)METHYL)BENZOATE                           
REMARK 900 RELATED ID: 3ZUN   RELATED DB: PDB                                   
REMARK 900  PVHL54-213-ELOB-ELOC COMPLEX_(2S,4R)-4-HYDROXY-1-(                  
REMARK 900  2-(3-METHYLISOXAZOL-5-YL)ACETYL)-N-(4-NITROBENZYL)                  
REMARK 900  PYRROLIDINE-2-CARBOXAMIDE BOUND                                     
DBREF  4AJY B    1   118  UNP    Q15370   ELOB_HUMAN       1    118             
DBREF  4AJY C   17   112  UNP    Q15369   ELOC_HUMAN       1     96             
DBREF  4AJY H  559   577  UNP    Q16665   HIF1A_HUMAN    559    577             
DBREF  4AJY V   54   213  UNP    P40337   VHL_HUMAN      214    373             
SEQADV 4AJY MET C   16  UNP  Q15369              EXPRESSION TAG                 
SEQADV 4AJY ASP H  559  UNP  Q16665    LEU   559 ENGINEERED MUTATION            
SEQADV 4AJY ALA H  561  UNP  Q16665    MET   561 ENGINEERED MUTATION            
SEQADV 4AJY GLY V   51  UNP  P40337              EXPRESSION TAG                 
SEQADV 4AJY SER V   52  UNP  P40337              EXPRESSION TAG                 
SEQADV 4AJY HIS V   53  UNP  P40337              EXPRESSION TAG                 
SEQRES   1 B  118  MET ASP VAL PHE LEU MET ILE ARG ARG HIS LYS THR THR          
SEQRES   2 B  118  ILE PHE THR ASP ALA LYS GLU SER SER THR VAL PHE GLU          
SEQRES   3 B  118  LEU LYS ARG ILE VAL GLU GLY ILE LEU LYS ARG PRO PRO          
SEQRES   4 B  118  ASP GLU GLN ARG LEU TYR LYS ASP ASP GLN LEU LEU ASP          
SEQRES   5 B  118  ASP GLY LYS THR LEU GLY GLU CYS GLY PHE THR SER GLN          
SEQRES   6 B  118  THR ALA ARG PRO GLN ALA PRO ALA THR VAL GLY LEU ALA          
SEQRES   7 B  118  PHE ARG ALA ASP ASP THR PHE GLU ALA LEU CYS ILE GLU          
SEQRES   8 B  118  PRO PHE SER SER PRO PRO GLU LEU PRO ASP VAL MET LYS          
SEQRES   9 B  118  PRO GLN ASP SER GLY SER SER ALA ASN GLU GLN ALA VAL          
SEQRES  10 B  118  GLN                                                          
SEQRES   1 C   97  MET MET TYR VAL LYS LEU ILE SER SER ASP GLY HIS GLU          
SEQRES   2 C   97  PHE ILE VAL LYS ARG GLU HIS ALA LEU THR SER GLY THR          
SEQRES   3 C   97  ILE LYS ALA MET LEU SER GLY PRO GLY GLN PHE ALA GLU          
SEQRES   4 C   97  ASN GLU THR ASN GLU VAL ASN PHE ARG GLU ILE PRO SER          
SEQRES   5 C   97  HIS VAL LEU SER LYS VAL CYS MET TYR PHE THR TYR LYS          
SEQRES   6 C   97  VAL ARG TYR THR ASN SER SER THR GLU ILE PRO GLU PHE          
SEQRES   7 C   97  PRO ILE ALA PRO GLU ILE ALA LEU GLU LEU LEU MET ALA          
SEQRES   8 C   97  ALA ASN PHE LEU ASP CYS                                      
SEQRES   1 H   19  ASP GLU ALA LEU ALA HYP TYR ILE PRO MET ASP ASP ASP          
SEQRES   2 H   19  PHE GLN LEU ARG SER PHE                                      
SEQRES   1 V  163  GLY SER HIS MET GLU ALA GLY ARG PRO ARG PRO VAL LEU          
SEQRES   2 V  163  ARG SER VAL ASN SER ARG GLU PRO SER GLN VAL ILE PHE          
SEQRES   3 V  163  CYS ASN ARG SER PRO ARG VAL VAL LEU PRO VAL TRP LEU          
SEQRES   4 V  163  ASN PHE ASP GLY GLU PRO GLN PRO TYR PRO THR LEU PRO          
SEQRES   5 V  163  PRO GLY THR GLY ARG ARG ILE HIS SER TYR ARG GLY HIS          
SEQRES   6 V  163  LEU TRP LEU PHE ARG ASP ALA GLY THR HIS ASP GLY LEU          
SEQRES   7 V  163  LEU VAL ASN GLN THR GLU LEU PHE VAL PRO SER LEU ASN          
SEQRES   8 V  163  VAL ASP GLY GLN PRO ILE PHE ALA ASN ILE THR LEU PRO          
SEQRES   9 V  163  VAL TYR THR LEU LYS GLU ARG CYS LEU GLN VAL VAL ARG          
SEQRES  10 V  163  SER LEU VAL LYS PRO GLU ASN TYR ARG ARG LEU ASP ILE          
SEQRES  11 V  163  VAL ARG SER LEU TYR GLU ASP LEU GLU ASP HIS PRO ASN          
SEQRES  12 V  163  VAL GLN LYS ASP LEU GLU ARG LEU THR GLN GLU ARG ILE          
SEQRES  13 V  163  ALA HIS GLN ARG MET GLY ASP                                  
MODRES 4AJY HYP H  564  PRO  4-HYDROXYPROLINE                                   
HET    GOL  V1208       6                                                       
HET    HYP  H 564       8                                                       
HETNAM     GOL GLYCEROL                                                         
HETNAM     HYP 4-HYDROXYPROLINE                                                 
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
HETSYN     HYP HYDROXYPROLINE                                                   
FORMUL   5  GOL    C3 H8 O3                                                     
FORMUL   6  HYP    C5 H9 N O3                                                   
FORMUL   7  HOH   *242(H2 O)                                                    
HELIX    1   1 THR B   23  LYS B   36  1                                  14    
HELIX    2   2 PRO B   38  ASP B   40  5                                   3    
HELIX    3   3 THR B   63  ALA B   67  5                                   5    
HELIX    4   4 PRO B  100  LYS B  104  5                                   5    
HELIX    5   5 ARG C   33  LEU C   37  1                                   5    
HELIX    6   6 SER C   39  SER C   47  1                                   9    
HELIX    7   7 PRO C   66  THR C   84  1                                  19    
HELIX    8   8 ALA C   96  GLU C   98  5                                   3    
HELIX    9   9 ILE C   99  ASP C  111  1                                  13    
HELIX   10  10 THR V  157  VAL V  170  1                                  14    
HELIX   11  11 LYS V  171  LEU V  178  5                                   8    
HELIX   12  12 ARG V  182  ASP V  190  1                                   9    
HELIX   13  13 ASN V  193  ALA V  207  1                                  15    
SHEET    1  BA 8 GLN B  49  LEU B  50  0                                        
SHEET    2  BA 8 GLN B  42  LYS B  46 -1  O  LYS B  46   N  GLN B  49           
SHEET    3  BA 8 ALA B  73  PHE B  79 -1  O  GLY B  76   N  TYR B  45           
SHEET    4  BA 8 ASP B   2  ARG B   9  1  O  PHE B   4   N  ALA B  73           
SHEET    5  BA 8 THR B  12  LYS B  19 -1  O  THR B  12   N  ARG B   9           
SHEET    6  BA 8 GLU C  28  LYS C  32  1  O  GLU C  28   N  THR B  13           
SHEET    7  BA 8 TYR C  18  ILE C  22 -1  O  VAL C  19   N  VAL C  31           
SHEET    8  BA 8 GLU C  59  ASN C  61  1  O  VAL C  60   N  ILE C  22           
SHEET    1  HA 5 PHE H 572  GLN H 573  0                                        
SHEET    2  HA 5 THR V 105  TYR V 112 -1  O  GLY V 106   N  PHE H 572           
SHEET    3  HA 5 PRO V  71  ASN V  78 -1  O  SER V  72   N  SER V 111           
SHEET    4  HA 5 ILE V 147  THR V 152  1  O  ILE V 147   N  ILE V  75           
SHEET    5  HA 5 LEU V 129  VAL V 130 -1  O  LEU V 129   N  THR V 152           
SHEET    1  VA 3 PRO V  95  PRO V  97  0                                        
SHEET    2  VA 3 VAL V  84  LEU V  89 -1  O  TRP V  88   N  GLN V  96           
SHEET    3  VA 3 LEU V 116  ASP V 121 -1  O  LEU V 116   N  LEU V  89           
LINK         N   HYP H 564                 C   ALA H 563     1555   1555  1.51  
LINK         C   HYP H 564                 N   TYR H 565     1555   1555  1.45  
SITE     1 AC1  5 GLU C  92  ARG V  82  ARG V 161  HOH V2027                    
SITE     2 AC1  5 HOH V2046                                                     
CRYST1   59.232   59.232  244.038  90.00  90.00  90.00 P 43 21 2     8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016883  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.016883  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.004098        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system