HEADER VIRUS 14-OCT-13 4CBF
TITLE NEAR-ATOMIC RESOLUTION CRYO-EM STRUCTURE OF DENGUE SEROTYPE 4 VIRUS
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: ENVELOPE PROTEIN E;
COMPND 3 CHAIN: A, C, E;
COMPND 4 ENGINEERED: YES;
COMPND 5 MOL_ID: 2;
COMPND 6 MOLECULE: M PROTEIN;
COMPND 7 CHAIN: B, D, F;
COMPND 8 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: DENGUE VIRUS 2;
SOURCE 3 ORGANISM_TAXID: 11060;
SOURCE 4 STRAIN: THAILAND/NGS-C/1944 (DENV-2);
SOURCE 5 EXPRESSION_SYSTEM: DROSOPHILA MELANOGASTER;
SOURCE 6 EXPRESSION_SYSTEM_TAXID: 7227;
SOURCE 7 EXPRESSION_SYSTEM_CELL_LINE: C6/36;
SOURCE 8 MOL_ID: 2;
SOURCE 9 SYNTHETIC: YES;
SOURCE 10 ORGANISM_SCIENTIFIC: DENGUE VIRUS 4;
SOURCE 11 ORGANISM_TAXID: 11070
KEYWDS FLAVIVIRUS, VIRUS
EXPDTA ELECTRON MICROSCOPY
MDLTYP CA ATOMS ONLY, CHAIN C, A, E, B, D, F
AUTHOR V.A.KOSTYUCHENKO,P.L.CHEW,T.S.NG,S.M.LOK
REVDAT 4 23-AUG-17 4CBF 1 REMARK
REVDAT 3 22-APR-15 4CBF 1 COMPND SOURCE DBREF SEQRES
REVDAT 3 2 1 ATOM
REVDAT 2 08-JAN-14 4CBF 1 JRNL
REVDAT 1 06-NOV-13 4CBF 0
JRNL AUTH V.A.KOSTYUCHENKO,P.L.CHEW,T.NG,S.LOK
JRNL TITL NEAR-ATOMIC RESOLUTION CRYO-ELECTRON MICROSCOPIC STRUCTURE
JRNL TITL 2 OF DENGUE SEROTYPE 4 VIRUS.
JRNL REF J.VIROL. V. 88 477 2014
JRNL REFN ISSN 0022-538X
JRNL PMID 24155405
JRNL DOI 10.1128/JVI.02641-13
REMARK 2
REMARK 2 RESOLUTION. 4.10 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 SOFTWARE PACKAGES : CNS, COOT, UCSF CHIMERA, EMAN, EMAN,
REMARK 3 MPSA
REMARK 3 RECONSTRUCTION SCHEMA : NULL
REMARK 3
REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT
REMARK 3 PDB ENTRY : 3J27
REMARK 3 REFINEMENT SPACE : REAL
REMARK 3 REFINEMENT PROTOCOL : FLEXIBLE FIT
REMARK 3 REFINEMENT TARGET : REAL SPACE CORRELATION
REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL
REMARK 3
REMARK 3 FITTING PROCEDURE : METHOD--FLEXIBLE REFINEMENT PROTOCOL--CRYO-EM
REMARK 3
REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS
REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : 1.080
REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 4.100
REMARK 3 NUMBER OF PARTICLES : 16602
REMARK 3 CTF CORRECTION METHOD : NULL
REMARK 3
REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: GRAPHITE SHEETS
REMARK 3
REMARK 3 OTHER DETAILS: MPSA SUBMISSION BASED ON EXPERIMENTAL DATA FROM
REMARK 3 EMDB EMD-2485. (DEPOSITION ID: 12027).
REMARK 4
REMARK 4 4CBF COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE.
REMARK 100 THE DEPOSITION ID IS D_1290058717.
REMARK 245
REMARK 245 EXPERIMENTAL DETAILS
REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE
REMARK 245 SPECIMEN TYPE : VITREOUS ICE CRYO EM
REMARK 245
REMARK 245 ELECTRON MICROSCOPE SAMPLE
REMARK 245 SAMPLE TYPE : PARTICLE
REMARK 245 PARTICLE TYPE : POINT
REMARK 245 NAME OF SAMPLE : MATURE DENGUE VIRUS SEROTYPE 4
REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL
REMARK 245 SAMPLE SUPPORT DETAILS : HOLEY CARBON
REMARK 245 SAMPLE VITRIFICATION DETAILS : LIQUID ETHANE
REMARK 245 SAMPLE BUFFER : NULL
REMARK 245 PH : 8.00
REMARK 245 SAMPLE DETAILS : NULL
REMARK 245
REMARK 245 DATA ACQUISITION
REMARK 245 DATE OF EXPERIMENT : 14-APR-12
REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL
REMARK 245 TEMPERATURE (KELVIN) : 100.00
REMARK 245 MICROSCOPE MODEL : FEI TITAN KRIOS
REMARK 245 DETECTOR TYPE : FEI FALCON I (4K X 4K)
REMARK 245 MINIMUM DEFOCUS (NM) : 500.00
REMARK 245 MAXIMUM DEFOCUS (NM) : 3500.00
REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 NOMINAL CS : 2.70
REMARK 245 IMAGING MODE : BRIGHT FIELD
REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 18.00
REMARK 245 ILLUMINATION MODE : FLOOD BEAM
REMARK 245 NOMINAL MAGNIFICATION : 125000
REMARK 245 CALIBRATED MAGNIFICATION : 129629
REMARK 245 SOURCE : FIELD EMISSION GUN
REMARK 245 ACCELERATION VOLTAGE (KV) : 300
REMARK 245 IMAGING DETAILS : NULL
REMARK 247
REMARK 247 ELECTRON MICROSCOPY
REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON
REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE
REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES
REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION
REMARK 247 OF THE STRUCTURE FACTORS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 300 THE ASSEMBLY REPRESENTED IN THIS ENTRY HAS REGULAR
REMARK 300 ICOSAHEDRAL POINT SYMMETRY (SCHOENFLIES SYMBOL = I).
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 350 BIOMT1 2 0.309017 -0.951057 0.000000 -0.00002
REMARK 350 BIOMT2 2 0.951057 0.309017 0.000000 0.00001
REMARK 350 BIOMT3 2 0.000000 0.000000 1.000000 0.00000
REMARK 350 BIOMT1 3 -0.809017 -0.587785 0.000000 -0.00004
REMARK 350 BIOMT2 3 0.587785 -0.809017 0.000000 0.00000
REMARK 350 BIOMT3 3 0.000000 0.000000 1.000000 0.00000
REMARK 350 BIOMT1 4 -0.809017 0.587785 0.000000 -0.00003
REMARK 350 BIOMT2 4 -0.587785 -0.809017 0.000000 -0.00002
REMARK 350 BIOMT3 4 0.000000 0.000000 1.000000 0.00000
REMARK 350 BIOMT1 5 0.309017 0.951057 0.000000 0.00000
REMARK 350 BIOMT2 5 -0.951057 0.309017 0.000000 -0.00002
REMARK 350 BIOMT3 5 0.000000 0.000000 1.000000 0.00000
REMARK 350 BIOMT1 6 -0.947214 -0.162460 0.276393 -0.00004
REMARK 350 BIOMT2 6 -0.162460 -0.500000 -0.850651 -0.00001
REMARK 350 BIOMT3 6 0.276393 -0.850651 0.447214 0.00000
REMARK 350 BIOMT1 7 -0.447214 0.850651 0.276393 -0.00002
REMARK 350 BIOMT2 7 -0.525731 0.000000 -0.850651 -0.00002
REMARK 350 BIOMT3 7 -0.723607 -0.525731 0.447214 -0.00002
REMARK 350 BIOMT1 8 0.670820 0.688191 0.276393 0.00000
REMARK 350 BIOMT2 8 -0.162460 0.500000 -0.850651 0.00000
REMARK 350 BIOMT3 8 -0.723607 0.525731 0.447214 -0.00001
REMARK 350 BIOMT1 9 0.861803 -0.425325 0.276393 0.00000
REMARK 350 BIOMT2 9 0.425325 0.309017 -0.850651 0.00000
REMARK 350 BIOMT3 9 0.276393 0.850651 0.447214 0.00001
REMARK 350 BIOMT1 10 -0.138197 -0.951057 0.276393 -0.00003
REMARK 350 BIOMT2 10 0.425325 -0.309017 -0.850651 0.00000
REMARK 350 BIOMT3 10 0.894427 0.000000 0.447214 0.00002
REMARK 350 BIOMT1 11 -0.861803 -0.425326 -0.276393 -0.00004
REMARK 350 BIOMT2 11 -0.425326 0.309017 0.850651 -0.00001
REMARK 350 BIOMT3 11 -0.276393 0.850651 -0.447214 0.00000
REMARK 350 BIOMT1 12 -0.670820 0.688191 -0.276393 -0.00003
REMARK 350 BIOMT2 12 0.162460 0.500000 0.850651 0.00000
REMARK 350 BIOMT3 12 0.723607 0.525731 -0.447214 0.00002
REMARK 350 BIOMT1 13 0.447214 0.850651 -0.276393 0.00000
REMARK 350 BIOMT2 13 0.525731 0.000000 0.850651 0.00000
REMARK 350 BIOMT3 13 0.723607 -0.525731 -0.447214 0.00001
REMARK 350 BIOMT1 14 0.947214 -0.162460 -0.276393 0.00000
REMARK 350 BIOMT2 14 0.162460 -0.500000 0.850651 0.00000
REMARK 350 BIOMT3 14 -0.276393 -0.850651 -0.447214 -0.00001
REMARK 350 BIOMT1 15 0.138197 -0.951056 -0.276393 -0.00002
REMARK 350 BIOMT2 15 -0.425325 -0.309017 0.850651 -0.00002
REMARK 350 BIOMT3 15 -0.894427 0.000000 -0.447214 -0.00002
REMARK 350 BIOMT1 16 0.809017 0.587785 0.000000 0.00000
REMARK 350 BIOMT2 16 0.587785 -0.809017 0.000000 0.00000
REMARK 350 BIOMT3 16 0.000000 0.000000 -1.000000 0.00000
REMARK 350 BIOMT1 17 0.809017 -0.587785 0.000000 0.00000
REMARK 350 BIOMT2 17 -0.587785 -0.809017 0.000000 -0.00002
REMARK 350 BIOMT3 17 0.000000 0.000000 -1.000000 0.00000
REMARK 350 BIOMT1 18 -0.309017 -0.951057 0.000000 -0.00003
REMARK 350 BIOMT2 18 -0.951057 0.309017 0.000000 -0.00002
REMARK 350 BIOMT3 18 0.000000 0.000000 -1.000000 0.00000
REMARK 350 BIOMT1 19 -1.000000 0.000000 0.000000 -0.00004
REMARK 350 BIOMT2 19 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 19 0.000000 0.000000 -1.000000 0.00000
REMARK 350 BIOMT1 20 -0.309017 0.951056 0.000000 -0.00002
REMARK 350 BIOMT2 20 0.951056 0.309017 0.000000 0.00001
REMARK 350 BIOMT3 20 0.000000 0.000000 -1.000000 0.00000
REMARK 350 BIOMT1 21 -0.138197 -0.425325 0.894427 -0.00002
REMARK 350 BIOMT2 21 0.951057 -0.309017 0.000000 0.00000
REMARK 350 BIOMT3 21 0.276393 0.850651 0.447214 0.00001
REMARK 350 BIOMT1 22 -0.447214 0.000000 0.894427 -0.00003
REMARK 350 BIOMT2 22 0.000000 -1.000000 0.000000 -0.00001
REMARK 350 BIOMT3 22 0.894427 0.000000 0.447214 0.00002
REMARK 350 BIOMT1 23 -0.138197 0.425325 0.894427 -0.00002
REMARK 350 BIOMT2 23 -0.951057 -0.309017 0.000000 -0.00003
REMARK 350 BIOMT3 23 0.276393 -0.850651 0.447214 0.00000
REMARK 350 BIOMT1 24 0.361803 0.262866 0.894427 -0.00001
REMARK 350 BIOMT2 24 -0.587785 0.809017 0.000000 -0.00001
REMARK 350 BIOMT3 24 -0.723607 -0.525731 0.447214 -0.00002
REMARK 350 BIOMT1 25 0.361803 -0.262866 0.894427 -0.00001
REMARK 350 BIOMT2 25 0.587785 0.809017 0.000000 0.00000
REMARK 350 BIOMT3 25 -0.723607 0.525731 0.447214 -0.00001
REMARK 350 BIOMT1 26 0.447214 -0.525731 0.723607 -0.00001
REMARK 350 BIOMT2 26 -0.850651 0.000000 0.525731 -0.00002
REMARK 350 BIOMT3 26 -0.276393 -0.850651 -0.447214 -0.00001
REMARK 350 BIOMT1 27 -0.361803 -0.587785 0.723607 -0.00003
REMARK 350 BIOMT2 27 -0.262866 0.809017 0.525731 0.00000
REMARK 350 BIOMT3 27 -0.894427 0.000000 -0.447214 -0.00002
REMARK 350 BIOMT1 28 -0.670820 0.162460 0.723607 -0.00003
REMARK 350 BIOMT2 28 0.688191 0.500000 0.525731 0.00000
REMARK 350 BIOMT3 28 -0.276393 0.850651 -0.447214 0.00000
REMARK 350 BIOMT1 29 -0.052787 0.688191 0.723607 -0.00002
REMARK 350 BIOMT2 29 0.688191 -0.500000 0.525731 0.00000
REMARK 350 BIOMT3 29 0.723607 0.525731 -0.447214 0.00002
REMARK 350 BIOMT1 30 0.638197 0.262866 0.723607 0.00000
REMARK 350 BIOMT2 30 -0.262866 -0.809017 0.525731 -0.00002
REMARK 350 BIOMT3 30 0.723607 -0.525731 -0.447214 0.00001
REMARK 350 BIOMT1 31 0.052786 0.688191 -0.723607 -0.00001
REMARK 350 BIOMT2 31 -0.688191 -0.500000 -0.525731 -0.00002
REMARK 350 BIOMT3 31 -0.723607 0.525731 0.447214 -0.00001
REMARK 350 BIOMT1 32 0.670820 0.162460 -0.723607 0.00000
REMARK 350 BIOMT2 32 -0.688191 0.500000 -0.525731 -0.00002
REMARK 350 BIOMT3 32 0.276393 0.850651 0.447214 0.00001
REMARK 350 BIOMT1 33 0.361803 -0.587785 -0.723607 -0.00002
REMARK 350 BIOMT2 33 0.262866 0.809017 -0.525731 0.00000
REMARK 350 BIOMT3 33 0.894427 0.000000 0.447214 0.00002
REMARK 350 BIOMT1 34 -0.447214 -0.525731 -0.723607 -0.00003
REMARK 350 BIOMT2 34 0.850651 0.000000 -0.525731 0.00000
REMARK 350 BIOMT3 34 0.276393 -0.850651 0.447214 0.00000
REMARK 350 BIOMT1 35 -0.638197 0.262866 -0.723607 -0.00003
REMARK 350 BIOMT2 35 0.262866 -0.809017 -0.525731 0.00000
REMARK 350 BIOMT3 35 -0.723607 -0.525731 0.447214 -0.00002
REMARK 350 BIOMT1 36 -0.361803 0.262866 -0.894427 -0.00002
REMARK 350 BIOMT2 36 0.587785 0.809017 0.000000 0.00000
REMARK 350 BIOMT3 36 0.723607 -0.525731 -0.447214 0.00001
REMARK 350 BIOMT1 37 0.138197 0.425325 -0.894427 -0.00001
REMARK 350 BIOMT2 37 0.951057 -0.309017 0.000000 0.00000
REMARK 350 BIOMT3 37 -0.276393 -0.850651 -0.447214 -0.00001
REMARK 350 BIOMT1 38 0.447214 0.000000 -0.894427 -0.00001
REMARK 350 BIOMT2 38 0.000000 -1.000000 0.000000 -0.00001
REMARK 350 BIOMT3 38 -0.894427 0.000000 -0.447214 -0.00002
REMARK 350 BIOMT1 39 0.138197 -0.425325 -0.894427 -0.00002
REMARK 350 BIOMT2 39 -0.951056 -0.309017 0.000000 -0.00003
REMARK 350 BIOMT3 39 -0.276393 0.850651 -0.447214 0.00000
REMARK 350 BIOMT1 40 -0.361803 -0.262866 -0.894427 -0.00003
REMARK 350 BIOMT2 40 -0.587785 0.809017 0.000000 -0.00001
REMARK 350 BIOMT3 40 0.723607 0.525731 -0.447214 0.00002
REMARK 350 BIOMT1 41 -0.138197 0.951057 0.276393 -0.00002
REMARK 350 BIOMT2 41 -0.425325 -0.309017 0.850651 -0.00002
REMARK 350 BIOMT3 41 0.894427 0.000000 0.447214 0.00002
REMARK 350 BIOMT1 42 0.861803 0.425325 0.276393 0.00000
REMARK 350 BIOMT2 42 -0.425325 0.309017 0.850651 -0.00001
REMARK 350 BIOMT3 42 0.276393 -0.850651 0.447214 0.00000
REMARK 350 BIOMT1 43 0.670820 -0.688191 0.276393 -0.00001
REMARK 350 BIOMT2 43 0.162460 0.500000 0.850651 0.00000
REMARK 350 BIOMT3 43 -0.723607 -0.525731 0.447214 -0.00002
REMARK 350 BIOMT1 44 -0.447214 -0.850651 0.276393 -0.00003
REMARK 350 BIOMT2 44 0.525731 0.000000 0.850651 0.00000
REMARK 350 BIOMT3 44 -0.723607 0.525731 0.447214 -0.00001
REMARK 350 BIOMT1 45 -0.947214 0.162460 0.276393 -0.00004
REMARK 350 BIOMT2 45 0.162460 -0.500000 0.850651 0.00000
REMARK 350 BIOMT3 45 0.276393 0.850651 0.447214 0.00001
REMARK 350 BIOMT1 46 0.052786 -0.688191 -0.723607 -0.00002
REMARK 350 BIOMT2 46 0.688191 -0.500000 0.525731 0.00000
REMARK 350 BIOMT3 46 -0.723607 -0.525731 0.447214 -0.00002
REMARK 350 BIOMT1 47 -0.638197 -0.262866 -0.723607 -0.00003
REMARK 350 BIOMT2 47 -0.262866 -0.809017 0.525731 -0.00002
REMARK 350 BIOMT3 47 -0.723607 0.525731 0.447214 -0.00001
REMARK 350 BIOMT1 48 -0.447214 0.525731 -0.723607 -0.00002
REMARK 350 BIOMT2 48 -0.850651 0.000000 0.525731 -0.00002
REMARK 350 BIOMT3 48 0.276393 0.850651 0.447214 0.00001
REMARK 350 BIOMT1 49 0.361803 0.587785 -0.723607 0.00000
REMARK 350 BIOMT2 49 -0.262866 0.809017 0.525731 0.00000
REMARK 350 BIOMT3 49 0.894427 0.000000 0.447214 0.00002
REMARK 350 BIOMT1 50 0.670820 -0.162460 -0.723607 0.00000
REMARK 350 BIOMT2 50 0.688191 0.500000 0.525731 0.00000
REMARK 350 BIOMT3 50 0.276393 -0.850651 0.447214 0.00000
REMARK 350 BIOMT1 51 -0.361803 0.587785 0.723607 -0.00002
REMARK 350 BIOMT2 51 0.262866 0.809017 -0.525731 0.00000
REMARK 350 BIOMT3 51 -0.894427 0.000000 -0.447214 -0.00002
REMARK 350 BIOMT1 52 0.447214 0.525731 0.723607 0.00000
REMARK 350 BIOMT2 52 0.850651 0.000000 -0.525731 0.00000
REMARK 350 BIOMT3 52 -0.276393 0.850651 -0.447214 0.00000
REMARK 350 BIOMT1 53 0.638197 -0.262866 0.723607 0.00000
REMARK 350 BIOMT2 53 0.262866 -0.809017 -0.525731 0.00000
REMARK 350 BIOMT3 53 0.723607 0.525731 -0.447214 0.00002
REMARK 350 BIOMT1 54 -0.052786 -0.688191 0.723607 -0.00002
REMARK 350 BIOMT2 54 -0.688191 -0.500000 -0.525731 -0.00002
REMARK 350 BIOMT3 54 0.723607 -0.525731 -0.447214 0.00001
REMARK 350 BIOMT1 55 -0.670820 -0.162460 0.723607 -0.00003
REMARK 350 BIOMT2 55 -0.688191 0.500000 -0.525731 -0.00002
REMARK 350 BIOMT3 55 -0.276393 -0.850651 -0.447214 -0.00001
REMARK 350 BIOMT1 56 0.447214 -0.850651 -0.276393 -0.00002
REMARK 350 BIOMT2 56 -0.525731 0.000000 -0.850651 -0.00002
REMARK 350 BIOMT3 56 0.723607 0.525731 -0.447214 0.00002
REMARK 350 BIOMT1 57 -0.670820 -0.688191 -0.276393 -0.00004
REMARK 350 BIOMT2 57 -0.162460 0.500000 -0.850651 0.00000
REMARK 350 BIOMT3 57 0.723607 -0.525731 -0.447214 0.00001
REMARK 350 BIOMT1 58 -0.861803 0.425325 -0.276393 -0.00003
REMARK 350 BIOMT2 58 0.425325 0.309017 -0.850651 0.00000
REMARK 350 BIOMT3 58 -0.276393 -0.850651 -0.447214 -0.00001
REMARK 350 BIOMT1 59 0.138197 0.951057 -0.276393 -0.00001
REMARK 350 BIOMT2 59 0.425325 -0.309017 -0.850651 0.00000
REMARK 350 BIOMT3 59 -0.894427 0.000000 -0.447214 -0.00002
REMARK 350 BIOMT1 60 0.947214 0.162460 -0.276393 0.00000
REMARK 350 BIOMT2 60 -0.162460 -0.500000 -0.850651 -0.00001
REMARK 350 BIOMT3 60 -0.276393 0.850651 -0.447214 0.00000
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: EMD-2485 RELATED DB: EMDB
REMARK 900 NEAR-ATOMIC RESOLUTION CRYO-EM STRUCTURE OF DENGUE SEROTYPE 4 VIRUS
REMARK 900 RELATED ID: 4CAU RELATED DB: PDB
REMARK 900 THREE-DIMENSIONAL STRUCTURE OF DENGUE VIRUS SEROTYPE 1 COMPLEXED
REMARK 900 WITH 2 HMAB 14C10 FAB
DBREF 4CBF A 1 324 UNP E0WXI2 E0WXI2_9FLAV 280 774
DBREF 4CBF B 1 59 UNP A4GV96 A4GV96_9FLAV 92 165
DBREF 4CBF C 1 324 UNP E0WXI2 E0WXI2_9FLAV 280 774
DBREF 4CBF D 1 59 UNP A4GV96 A4GV96_9FLAV 92 165
DBREF 4CBF E 1 324 UNP E0WXI2 E0WXI2_9FLAV 280 774
DBREF 4CBF F 1 59 UNP A4GV96 A4GV96_9FLAV 92 165
SEQRES 1 A 495 MET ARG CYS VAL GLY VAL GLY ASN ARG ASP PHE VAL GLU
SEQRES 2 A 495 GLY VAL SER GLY GLY ALA TRP VAL ASP LEU VAL LEU GLU
SEQRES 3 A 495 HIS GLY GLY CYS VAL THR THR MET ALA GLN GLY LYS PRO
SEQRES 4 A 495 THR LEU ASP PHE GLU LEU THR LYS THR THR ALA LYS GLU
SEQRES 5 A 495 VAL ALA LEU LEU ARG THR TYR CYS ILE GLU ALA SER ILE
SEQRES 6 A 495 SER ASN ILE THR THR ALA THR ARG CYS PRO THR GLN GLY
SEQRES 7 A 495 GLU PRO TYR LEU LYS GLU GLU GLN ASP GLN GLN TYR ILE
SEQRES 8 A 495 CYS ARG ARG ASP VAL VAL ASP ARG GLY TRP GLY ASN GLY
SEQRES 9 A 495 CYS GLY LEU PHE GLY LYS GLY GLY VAL VAL THR CYS ALA
SEQRES 10 A 495 LYS PHE LEU CYS SER GLY LYS ILE THR GLY ASN LEU VAL
SEQRES 11 A 495 GLN ILE GLU ASN LEU GLU TYR THR VAL ALA VAL THR VAL
SEQRES 12 A 495 HIS ASN GLY ASP THR HIS ALA VAL GLY ASN ASP THR SER
SEQRES 13 A 495 SER HIS GLY VAL THR ALA THR ILE THR PRO ARG SER PRO
SEQRES 14 A 495 SER VAL GLU VAL LYS LEU PRO ASP TYR GLY GLU LEU THR
SEQRES 15 A 495 LEU ASP CYS GLU PRO ARG SER GLY ILE ASP PHE ASN GLU
SEQRES 16 A 495 MET ILE LEU MET LYS MET LYS LYS LYS THR TRP LEU VAL
SEQRES 17 A 495 HIS LYS GLN TRP PHE LEU ASP LEU PRO LEU PRO TRP THR
SEQRES 18 A 495 ALA GLY ALA ASP THR SER GLU VAL HIS TRP ASN HIS LYS
SEQRES 19 A 495 GLU ARG MET VAL THR PHE LYS VAL PRO HIS ALA LYS ARG
SEQRES 20 A 495 GLN ASP VAL THR VAL LEU GLY SER GLN GLU GLY ALA MET
SEQRES 21 A 495 HIS SER ALA LEU ALA GLY ALA THR GLU VAL ASP SER GLY
SEQRES 22 A 495 ASP GLY ASN HIS MET PHE ALA GLY HIS LEU LYS CYS LYS
SEQRES 23 A 495 VAL ARG MET GLU LYS LEU ARG ILE LYS GLY MET SER TYR
SEQRES 24 A 495 THR MET CYS SER GLY LYS PHE SER ILE ASP LYS GLU MET
SEQRES 25 A 495 ALA GLU THR GLN HIS GLY THR THR VAL VAL LYS VAL LYS
SEQRES 26 A 495 TYR GLU GLY ALA GLY ALA PRO CYS LYS VAL PRO ILE GLU
SEQRES 27 A 495 ILE ARG ASP VAL ASN LYS GLU LYS VAL VAL GLY ARG ILE
SEQRES 28 A 495 ILE SER SER THR PRO PHE ALA GLU ASN THR ASN SER VAL
SEQRES 29 A 495 THR ASN ILE GLU LEU GLU PRO PRO PHE GLY ASP SER TYR
SEQRES 30 A 495 ILE VAL ILE GLY VAL GLY GLU SER ALA LEU THR LEU HIS
SEQRES 31 A 495 TRP PHE ARG LYS GLY SER SER ILE GLY LYS MET PHE GLU
SEQRES 32 A 495 SER THR TYR ARG GLY ALA LYS ARG MET ALA ILE LEU GLY
SEQRES 33 A 495 GLU THR ALA TRP ASP PHE GLY SER VAL GLY GLY LEU PHE
SEQRES 34 A 495 THR SER LEU GLY LYS ALA VAL HIS GLN VAL PHE GLY SER
SEQRES 35 A 495 VAL TYR THR THR MET PHE GLY GLY VAL SER TRP MET VAL
SEQRES 36 A 495 ARG ILE LEU ILE GLY PHE LEU VAL LEU TRP ILE GLY THR
SEQRES 37 A 495 ASN SER ARG ASN THR SER MET ALA MET THR CYS ILE ALA
SEQRES 38 A 495 VAL GLY GLY ILE THR LEU PHE LEU GLY PHE THR VAL GLN
SEQRES 39 A 495 ALA
SEQRES 1 B 74 SER VAL ALA LEU THR PRO HIS SER GLY MET GLY LEU GLU
SEQRES 2 B 74 THR ARG ALA GLU THR TRP MET SER SER GLU GLY ALA TRP
SEQRES 3 B 74 LYS HIS ALA GLN ARG VAL GLU SER TRP ILE LEU ARG ASN
SEQRES 4 B 74 PRO GLY PHE ALA LEU LEU ALA GLY PHE MET ALA TYR MET
SEQRES 5 B 74 ILE GLY GLN THR GLY ILE GLN ARG THR VAL PHE PHE VAL
SEQRES 6 B 74 LEU MET MET LEU VAL ALA PRO SER TYR
SEQRES 1 C 495 MET ARG CYS VAL GLY VAL GLY ASN ARG ASP PHE VAL GLU
SEQRES 2 C 495 GLY VAL SER GLY GLY ALA TRP VAL ASP LEU VAL LEU GLU
SEQRES 3 C 495 HIS GLY GLY CYS VAL THR THR MET ALA GLN GLY LYS PRO
SEQRES 4 C 495 THR LEU ASP PHE GLU LEU THR LYS THR THR ALA LYS GLU
SEQRES 5 C 495 VAL ALA LEU LEU ARG THR TYR CYS ILE GLU ALA SER ILE
SEQRES 6 C 495 SER ASN ILE THR THR ALA THR ARG CYS PRO THR GLN GLY
SEQRES 7 C 495 GLU PRO TYR LEU LYS GLU GLU GLN ASP GLN GLN TYR ILE
SEQRES 8 C 495 CYS ARG ARG ASP VAL VAL ASP ARG GLY TRP GLY ASN GLY
SEQRES 9 C 495 CYS GLY LEU PHE GLY LYS GLY GLY VAL VAL THR CYS ALA
SEQRES 10 C 495 LYS PHE LEU CYS SER GLY LYS ILE THR GLY ASN LEU VAL
SEQRES 11 C 495 GLN ILE GLU ASN LEU GLU TYR THR VAL ALA VAL THR VAL
SEQRES 12 C 495 HIS ASN GLY ASP THR HIS ALA VAL GLY ASN ASP THR SER
SEQRES 13 C 495 SER HIS GLY VAL THR ALA THR ILE THR PRO ARG SER PRO
SEQRES 14 C 495 SER VAL GLU VAL LYS LEU PRO ASP TYR GLY GLU LEU THR
SEQRES 15 C 495 LEU ASP CYS GLU PRO ARG SER GLY ILE ASP PHE ASN GLU
SEQRES 16 C 495 MET ILE LEU MET LYS MET LYS LYS LYS THR TRP LEU VAL
SEQRES 17 C 495 HIS LYS GLN TRP PHE LEU ASP LEU PRO LEU PRO TRP THR
SEQRES 18 C 495 ALA GLY ALA ASP THR SER GLU VAL HIS TRP ASN HIS LYS
SEQRES 19 C 495 GLU ARG MET VAL THR PHE LYS VAL PRO HIS ALA LYS ARG
SEQRES 20 C 495 GLN ASP VAL THR VAL LEU GLY SER GLN GLU GLY ALA MET
SEQRES 21 C 495 HIS SER ALA LEU ALA GLY ALA THR GLU VAL ASP SER GLY
SEQRES 22 C 495 ASP GLY ASN HIS MET PHE ALA GLY HIS LEU LYS CYS LYS
SEQRES 23 C 495 VAL ARG MET GLU LYS LEU ARG ILE LYS GLY MET SER TYR
SEQRES 24 C 495 THR MET CYS SER GLY LYS PHE SER ILE ASP LYS GLU MET
SEQRES 25 C 495 ALA GLU THR GLN HIS GLY THR THR VAL VAL LYS VAL LYS
SEQRES 26 C 495 TYR GLU GLY ALA GLY ALA PRO CYS LYS VAL PRO ILE GLU
SEQRES 27 C 495 ILE ARG ASP VAL ASN LYS GLU LYS VAL VAL GLY ARG ILE
SEQRES 28 C 495 ILE SER SER THR PRO PHE ALA GLU ASN THR ASN SER VAL
SEQRES 29 C 495 THR ASN ILE GLU LEU GLU PRO PRO PHE GLY ASP SER TYR
SEQRES 30 C 495 ILE VAL ILE GLY VAL GLY GLU SER ALA LEU THR LEU HIS
SEQRES 31 C 495 TRP PHE ARG LYS GLY SER SER ILE GLY LYS MET PHE GLU
SEQRES 32 C 495 SER THR TYR ARG GLY ALA LYS ARG MET ALA ILE LEU GLY
SEQRES 33 C 495 GLU THR ALA TRP ASP PHE GLY SER VAL GLY GLY LEU PHE
SEQRES 34 C 495 THR SER LEU GLY LYS ALA VAL HIS GLN VAL PHE GLY SER
SEQRES 35 C 495 VAL TYR THR THR MET PHE GLY GLY VAL SER TRP MET VAL
SEQRES 36 C 495 ARG ILE LEU ILE GLY PHE LEU VAL LEU TRP ILE GLY THR
SEQRES 37 C 495 ASN SER ARG ASN THR SER MET ALA MET THR CYS ILE ALA
SEQRES 38 C 495 VAL GLY GLY ILE THR LEU PHE LEU GLY PHE THR VAL GLN
SEQRES 39 C 495 ALA
SEQRES 1 D 74 SER VAL ALA LEU THR PRO HIS SER GLY MET GLY LEU GLU
SEQRES 2 D 74 THR ARG ALA GLU THR TRP MET SER SER GLU GLY ALA TRP
SEQRES 3 D 74 LYS HIS ALA GLN ARG VAL GLU SER TRP ILE LEU ARG ASN
SEQRES 4 D 74 PRO GLY PHE ALA LEU LEU ALA GLY PHE MET ALA TYR MET
SEQRES 5 D 74 ILE GLY GLN THR GLY ILE GLN ARG THR VAL PHE PHE VAL
SEQRES 6 D 74 LEU MET MET LEU VAL ALA PRO SER TYR
SEQRES 1 E 495 MET ARG CYS VAL GLY VAL GLY ASN ARG ASP PHE VAL GLU
SEQRES 2 E 495 GLY VAL SER GLY GLY ALA TRP VAL ASP LEU VAL LEU GLU
SEQRES 3 E 495 HIS GLY GLY CYS VAL THR THR MET ALA GLN GLY LYS PRO
SEQRES 4 E 495 THR LEU ASP PHE GLU LEU THR LYS THR THR ALA LYS GLU
SEQRES 5 E 495 VAL ALA LEU LEU ARG THR TYR CYS ILE GLU ALA SER ILE
SEQRES 6 E 495 SER ASN ILE THR THR ALA THR ARG CYS PRO THR GLN GLY
SEQRES 7 E 495 GLU PRO TYR LEU LYS GLU GLU GLN ASP GLN GLN TYR ILE
SEQRES 8 E 495 CYS ARG ARG ASP VAL VAL ASP ARG GLY TRP GLY ASN GLY
SEQRES 9 E 495 CYS GLY LEU PHE GLY LYS GLY GLY VAL VAL THR CYS ALA
SEQRES 10 E 495 LYS PHE LEU CYS SER GLY LYS ILE THR GLY ASN LEU VAL
SEQRES 11 E 495 GLN ILE GLU ASN LEU GLU TYR THR VAL ALA VAL THR VAL
SEQRES 12 E 495 HIS ASN GLY ASP THR HIS ALA VAL GLY ASN ASP THR SER
SEQRES 13 E 495 SER HIS GLY VAL THR ALA THR ILE THR PRO ARG SER PRO
SEQRES 14 E 495 SER VAL GLU VAL LYS LEU PRO ASP TYR GLY GLU LEU THR
SEQRES 15 E 495 LEU ASP CYS GLU PRO ARG SER GLY ILE ASP PHE ASN GLU
SEQRES 16 E 495 MET ILE LEU MET LYS MET LYS LYS LYS THR TRP LEU VAL
SEQRES 17 E 495 HIS LYS GLN TRP PHE LEU ASP LEU PRO LEU PRO TRP THR
SEQRES 18 E 495 ALA GLY ALA ASP THR SER GLU VAL HIS TRP ASN HIS LYS
SEQRES 19 E 495 GLU ARG MET VAL THR PHE LYS VAL PRO HIS ALA LYS ARG
SEQRES 20 E 495 GLN ASP VAL THR VAL LEU GLY SER GLN GLU GLY ALA MET
SEQRES 21 E 495 HIS SER ALA LEU ALA GLY ALA THR GLU VAL ASP SER GLY
SEQRES 22 E 495 ASP GLY ASN HIS MET PHE ALA GLY HIS LEU LYS CYS LYS
SEQRES 23 E 495 VAL ARG MET GLU LYS LEU ARG ILE LYS GLY MET SER TYR
SEQRES 24 E 495 THR MET CYS SER GLY LYS PHE SER ILE ASP LYS GLU MET
SEQRES 25 E 495 ALA GLU THR GLN HIS GLY THR THR VAL VAL LYS VAL LYS
SEQRES 26 E 495 TYR GLU GLY ALA GLY ALA PRO CYS LYS VAL PRO ILE GLU
SEQRES 27 E 495 ILE ARG ASP VAL ASN LYS GLU LYS VAL VAL GLY ARG ILE
SEQRES 28 E 495 ILE SER SER THR PRO PHE ALA GLU ASN THR ASN SER VAL
SEQRES 29 E 495 THR ASN ILE GLU LEU GLU PRO PRO PHE GLY ASP SER TYR
SEQRES 30 E 495 ILE VAL ILE GLY VAL GLY GLU SER ALA LEU THR LEU HIS
SEQRES 31 E 495 TRP PHE ARG LYS GLY SER SER ILE GLY LYS MET PHE GLU
SEQRES 32 E 495 SER THR TYR ARG GLY ALA LYS ARG MET ALA ILE LEU GLY
SEQRES 33 E 495 GLU THR ALA TRP ASP PHE GLY SER VAL GLY GLY LEU PHE
SEQRES 34 E 495 THR SER LEU GLY LYS ALA VAL HIS GLN VAL PHE GLY SER
SEQRES 35 E 495 VAL TYR THR THR MET PHE GLY GLY VAL SER TRP MET VAL
SEQRES 36 E 495 ARG ILE LEU ILE GLY PHE LEU VAL LEU TRP ILE GLY THR
SEQRES 37 E 495 ASN SER ARG ASN THR SER MET ALA MET THR CYS ILE ALA
SEQRES 38 E 495 VAL GLY GLY ILE THR LEU PHE LEU GLY PHE THR VAL GLN
SEQRES 39 E 495 ALA
SEQRES 1 F 74 SER VAL ALA LEU THR PRO HIS SER GLY MET GLY LEU GLU
SEQRES 2 F 74 THR ARG ALA GLU THR TRP MET SER SER GLU GLY ALA TRP
SEQRES 3 F 74 LYS HIS ALA GLN ARG VAL GLU SER TRP ILE LEU ARG ASN
SEQRES 4 F 74 PRO GLY PHE ALA LEU LEU ALA GLY PHE MET ALA TYR MET
SEQRES 5 F 74 ILE GLY GLN THR GLY ILE GLN ARG THR VAL PHE PHE VAL
SEQRES 6 F 74 LEU MET MET LEU VAL ALA PRO SER TYR
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
