GenomeNet

Database: PDB
Entry: 4GZN
LinkDB: 4GZN
Original site: 4GZN 
HEADER    TRANSCRIPTION/DNA                       06-SEP-12   4GZN              
TITLE     MOUSE ZFP57 ZINC FINGERS IN COMPLEX WITH METHYLATED DNA               
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DNA (5'-D(*TP*AP*TP*TP*GP*CP*(5CM)P*GP*CP*AP*G)-3');       
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: ZINC FINGER DOMAIN, UNP RESIDUES 137-195;                  
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: DNA (5'-D(*AP*CP*TP*GP*(5CM)P*GP*GP*CP*AP*AP*T)-3');       
COMPND   8 CHAIN: B;                                                            
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MOL_ID: 3;                                                           
COMPND  11 MOLECULE: ZINC FINGER PROTEIN 57;                                    
COMPND  12 CHAIN: C;                                                            
COMPND  13 SYNONYM: ZFP-57;                                                     
COMPND  14 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 OTHER_DETAILS: THE DNA SEQUENCE OCCURS NATURALLY IN MOUSE.;          
SOURCE   4 MOL_ID: 2;                                                           
SOURCE   5 SYNTHETIC: YES;                                                      
SOURCE   6 OTHER_DETAILS: THE DNA SEQUENCE OCCURS NATURALLY IN MOUSE.;          
SOURCE   7 MOL_ID: 3;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   9 ORGANISM_COMMON: MOUSE;                                              
SOURCE  10 ORGANISM_TAXID: 10090;                                               
SOURCE  11 GENE: ZFP57;                                                         
SOURCE  12 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  13 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    ZINC FINGER, TRANSCRIPTION-DNA COMPLEX                                
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Y.LIU,X.ZHANG,X.CHENG                                                 
REVDAT   2   21-NOV-12 4GZN    1       JRNL                                     
REVDAT   1   17-OCT-12 4GZN    0                                                
JRNL        AUTH   Y.LIU,H.TOH,H.SASAKI,X.ZHANG,X.CHENG                         
JRNL        TITL   AN ATOMIC MODEL OF ZFP57 RECOGNITION OF CPG METHYLATION      
JRNL        TITL 2 WITHIN A SPECIFIC DNA SEQUENCE.                              
JRNL        REF    GENES DEV.                    V.  26  2374 2012              
JRNL        REFN                   ISSN 0890-9369                               
JRNL        PMID   23059534                                                     
JRNL        DOI    10.1101/GAD.202200.112                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION.    0.99 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.6.4_486)                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 0.99                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.68                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.020                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 93.2                           
REMARK   3   NUMBER OF REFLECTIONS             : 117899                         
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.132                           
REMARK   3   R VALUE            (WORKING SET) : 0.131                           
REMARK   3   FREE R VALUE                     : 0.140                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.070                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 5979                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 19.6867 -  2.1325    1.00    11997   607  0.1521 0.1527        
REMARK   3     2  2.1325 -  1.6929    1.00    12091   574  0.1270 0.1382        
REMARK   3     3  1.6929 -  1.4790    1.00    11983   664  0.1070 0.1228        
REMARK   3     4  1.4790 -  1.3438    1.00    11948   652  0.1032 0.1155        
REMARK   3     5  1.3438 -  1.2475    1.00    11958   672  0.1047 0.1127        
REMARK   3     6  1.2475 -  1.1740    0.99    11877   655  0.1031 0.1151        
REMARK   3     7  1.1740 -  1.1152    0.99    11909   607  0.1045 0.1207        
REMARK   3     8  1.1152 -  1.0666    0.97    11707   588  0.1279 0.1403        
REMARK   3     9  1.0666 -  1.0256    0.85    10204   565  0.1970 0.1963        
REMARK   3    10  1.0256 -  0.9902    0.52     6246   395  0.3251 0.3175        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 0.30                                          
REMARK   3   SHRINKAGE RADIUS   : 0.00                                          
REMARK   3   K_SOL              : 0.47                                          
REMARK   3   B_SOL              : 65.15                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.070            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 10.960           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 7.20                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 7.84                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 0.44320                                              
REMARK   3    B22 (A**2) : -0.59500                                             
REMARK   3    B33 (A**2) : 0.15190                                              
REMARK   3    B12 (A**2) : -0.00000                                             
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : -0.00000                                             
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.009           1203                                  
REMARK   3   ANGLE     :  1.799           1776                                  
REMARK   3   CHIRALITY :  0.075            200                                  
REMARK   3   PLANARITY :  0.010            137                                  
REMARK   3   DIHEDRAL  : 25.061            601                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4GZN COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 24-SEP-12.                  
REMARK 100 THE RCSB ID CODE IS RCSB074833.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 12-JAN-12                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 4.6                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 22-BM                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000                              
REMARK 200  MONOCHROMATOR                  : SI 111 CHANNEL                     
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 225 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 802194                             
REMARK 200  RESOLUTION RANGE HIGH      (A) : 0.990                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 48.600                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 94.4                               
REMARK 200  DATA REDUNDANCY                : 12.900                             
REMARK 200  R MERGE                    (I) : 0.13300                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 16.1000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 0.99                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.03                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 58.9                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.40400                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.800                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SAD                          
REMARK 200 SOFTWARE USED: PHENIX (PHENIX.AUTOSOL)                               
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 42.12                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.13                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 25% MPD, 15% PEG8000, 100MM CACL2 AND    
REMARK 280  100 MM ACETATE PH 4.6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE   
REMARK 280  289K                                                                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       48.05950            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       48.05950            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       20.10900            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       30.32050            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       20.10900            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       30.32050            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       48.05950            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       20.10900            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       30.32050            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       48.05950            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       20.10900            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       30.32050            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 5250 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 6700 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -35.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, B, A                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ALA C   195                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     SER C 136    CB   OG                                             
REMARK 470     GLU C 137    CG   CD   OE1  OE2                                  
REMARK 470     LYS C 193    CG   CD   CE   NZ                                   
REMARK 470     PRO C 194    O    CB   CG   CD                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500     DT A   3   O3'    DT A   3   C3'    -0.047                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DT A   3   O5' -  C5' -  C4' ANGL. DEV. =  -8.2 DEGREES          
REMARK 500     DC A   9   O4' -  C1' -  N1  ANGL. DEV. =  -5.0 DEGREES          
REMARK 500     DA A  10   O4' -  C1' -  N9  ANGL. DEV. =  -4.4 DEGREES          
REMARK 500     DT B   3   O4' -  C1' -  N1  ANGL. DEV. =   2.1 DEGREES          
REMARK 500     DG B   7   O4' -  C1' -  N9  ANGL. DEV. =  -4.7 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH C 377        DISTANCE =  6.50 ANGSTROMS                       
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              ZN C 204  ZN                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HIS C 162   NE2                                                    
REMARK 620 2 HIS C 158   NE2  94.4                                              
REMARK 620 3 CYS C 145   SG  121.8 104.6                                        
REMARK 620 4 CYS C 142   SG  107.5 105.4 118.8                                  
REMARK 620 N                    1     2     3                                   
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              ZN C 203  ZN                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HIS C 190   NE2                                                    
REMARK 620 2 HIS C 186   NE2 107.9                                              
REMARK 620 3 CYS C 173   SG  112.5 106.7                                        
REMARK 620 4 CYS C 170   SG  108.9 105.9 114.5                                  
REMARK 620 N                    1     2     3                                   
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              CA B 101  CA                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH B 208   O                                                      
REMARK 620 2 HOH A 103   O   165.0                                              
REMARK 620 3 HOH B 206   O    96.7  87.8                                        
REMARK 620 4 HOH B 209   O    86.9  80.8  73.3                                  
REMARK 620 5 HOH B 204   O    81.9  87.3 151.4  78.1                            
REMARK 620 6 HOH A 101   O   111.0  76.5 127.9 147.6  78.0                      
REMARK 620 7 HOH B 203   O    81.7 113.2  71.6 141.3 135.6  70.0                
REMARK 620 N                    1     2     3     4     5     6                 
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CA B 101                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD C 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD C 202                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN C 203                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN C 204                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ACT C 205                 
DBREF  4GZN C  137   195  UNP    Q8C6P8   ZFP57_MOUSE    137    195             
DBREF  4GZN A    1    11  PDB    4GZN     4GZN             1     11             
DBREF  4GZN B    1    11  PDB    4GZN     4GZN             1     11             
SEQADV 4GZN SER C  136  UNP  Q8C6P8              EXPRESSION TAG                 
SEQRES   1 A   11   DT  DA  DT  DT  DG  DC 5CM  DG  DC  DA  DG                  
SEQRES   1 B   11   DA  DC  DT  DG 5CM  DG  DG  DC  DA  DA  DT                  
SEQRES   1 C   60  SER GLU ARG PRO PHE PHE CYS ASN PHE CYS GLY LYS THR          
SEQRES   2 C   60  TYR ARG ASP ALA SER GLY LEU SER ARG HIS ARG ARG ALA          
SEQRES   3 C   60  HIS LEU GLY TYR ARG PRO ARG SER CYS PRO GLU CYS GLY          
SEQRES   4 C   60  LYS CYS PHE ARG ASP GLN SER GLU VAL ASN ARG HIS LEU          
SEQRES   5 C   60  LYS VAL HIS GLN ASN LYS PRO ALA                              
MODRES 4GZN 5CM A    7   DC                                                     
MODRES 4GZN 5CM B    5   DC                                                     
HET    5CM  A   7      31                                                       
HET    5CM  B   5      20                                                       
HET     CA  B 101       1                                                       
HET    MPD  C 201       8                                                       
HET    MPD  C 202       8                                                       
HET     ZN  C 203       1                                                       
HET     ZN  C 204       1                                                       
HET    ACT  C 205       4                                                       
HETNAM     5CM 5-METHYL-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE                      
HETNAM      CA CALCIUM ION                                                      
HETNAM     MPD (4S)-2-METHYL-2,4-PENTANEDIOL                                    
HETNAM      ZN ZINC ION                                                         
HETNAM     ACT ACETATE ION                                                      
FORMUL   1  5CM    2(C10 H16 N3 O7 P)                                           
FORMUL   4   CA    CA 2+                                                        
FORMUL   5  MPD    2(C6 H14 O2)                                                 
FORMUL   7   ZN    2(ZN 2+)                                                     
FORMUL   9  ACT    C2 H3 O2 1-                                                  
FORMUL  10  HOH   *257(H2 O)                                                    
HELIX    1   1 ASP C  151  GLY C  164  1                                  14    
HELIX    2   2 ASP C  179  LEU C  187  1                                   9    
HELIX    3   3 LYS C  188  GLN C  191  5                                   4    
SHEET    1   A 2 PHE C 140  PHE C 141  0                                        
SHEET    2   A 2 THR C 148  TYR C 149 -1  O  TYR C 149   N  PHE C 140           
SHEET    1   B 2 ARG C 168  SER C 169  0                                        
SHEET    2   B 2 CYS C 176  PHE C 177 -1  O  PHE C 177   N  ARG C 168           
LINK         O3'A DC A   6                 P  A5CM A   7     1555   1555  1.60  
LINK         O3'B DC A   6                 P  B5CM A   7     1555   1555  1.61  
LINK         O3'A5CM A   7                 P  A DG A   8     1555   1555  1.61  
LINK         O3'B5CM A   7                 P  B DG A   8     1555   1555  1.61  
LINK         O3'  DG B   4                 P   5CM B   5     1555   1555  1.61  
LINK         O3' 5CM B   5                 P    DG B   6     1555   1555  1.61  
LINK         NE2 HIS C 162                ZN    ZN C 204     1555   1555  2.02  
LINK         NE2 HIS C 190                ZN    ZN C 203     1555   1555  2.04  
LINK         NE2 HIS C 186                ZN    ZN C 203     1555   1555  2.06  
LINK         NE2 HIS C 158                ZN    ZN C 204     1555   1555  2.06  
LINK         SG  CYS C 145                ZN    ZN C 204     1555   1555  2.26  
LINK         SG  CYS C 173                ZN    ZN C 203     1555   1555  2.30  
LINK         SG  CYS C 170                ZN    ZN C 203     1555   1555  2.31  
LINK         SG  CYS C 142                ZN    ZN C 204     1555   1555  2.32  
LINK        CA    CA B 101                 O   HOH B 208     1555   1555  2.37  
LINK        CA    CA B 101                 O   HOH A 103     1555   1555  2.40  
LINK        CA    CA B 101                 O   HOH B 206     1555   1555  2.41  
LINK        CA    CA B 101                 O   HOH B 209     1555   1555  2.43  
LINK        CA    CA B 101                 O   HOH B 204     1555   1555  2.43  
LINK        CA    CA B 101                 O   HOH A 101     1555   1555  2.44  
LINK        CA    CA B 101                 O   HOH B 203     1555   1555  2.46  
SITE     1 AC1  7 HOH A 101  HOH A 103  HOH B 203  HOH B 204                    
SITE     2 AC1  7 HOH B 206  HOH B 208  HOH B 209                               
SITE     1 AC2  7 HOH A 168  PHE C 141  ASN C 184  HOH C 313                    
SITE     2 AC2  7 HOH C 328  HOH C 329  HOH C 377                               
SITE     1 AC3  7 5CM B   5  HOH B 265  HIS C 158  ALA C 161                    
SITE     2 AC3  7 HIS C 162  GLY C 164  HOH C 333                               
SITE     1 AC4  4 CYS C 170  CYS C 173  HIS C 186  HIS C 190                    
SITE     1 AC5  4 CYS C 142  CYS C 145  HIS C 158  HIS C 162                    
SITE     1 AC6  7 HOH A 111  LEU C 163  TYR C 165  HIS C 190                    
SITE     2 AC6  7 HOH C 337  HOH C 352  HOH C 392                               
CRYST1   40.218   60.641   96.119  90.00  90.00  90.00 C 2 2 21      8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.024864  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.016490  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010404        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system