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Database: PDB
Entry: 4MHV
LinkDB: 4MHV
Original site: 4MHV 
HEADER    TRANSCRIPTION                           30-AUG-13   4MHV              
TITLE     CRYSTAL STRUCTURE OF THE PNT DOMAIN OF HUMAN ETS2                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN C-ETS-2;                                           
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: PNT DOMAIN (UNP RESIDUES 76-170);                          
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ETS2;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    PNT (POINTED) DOMAIN, TRANSCRIPTION, TRANSCRIPTION FACTOR             
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.A.NEWMAN,C.D.O.COOPER,T.KROJER,L.SHRESTHA,N.BURGESS-BROWN,          
AUTHOR   2 C.H.ARROWSMITH,C.BOUNTRA,A.EDWARDS,O.GILEADI                         
REVDAT   2   29-JAN-14 4MHV    1       DBREF  SEQADV REMARK HELIX               
REVDAT   2 2                   1       LINK   SITE                              
REVDAT   1   25-SEP-13 4MHV    0                                                
JRNL        AUTH   J.A.NEWMAN,C.D.O.COOPER,T.KROJER,L.SHRESTHA,N.BURGESS-BROWN, 
JRNL        AUTH 2 C.H.ARROWSMITH,C.BOUNTRA,A.EDWARDS,O.GILEADI                 
JRNL        TITL   CRYSTAL STRUCTURE OF THE PNT DOMAIN OF HUMAN ETS2            
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.45 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : BUSTER 2.11.4                                        
REMARK   3   AUTHORS     : BRICOGNE,BLANC,BRANDL,FLENSBURG,KELLER,              
REMARK   3               : PACIOREK,ROVERSI,SHARFF,SMART,VONRHEIN,              
REMARK   3               : WOMACK,MATTHEWS,TEN EYCK,TRONRUD                     
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.45                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.95                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.2                           
REMARK   3   NUMBER OF REFLECTIONS             : 12589                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD           : THROUGHOUT                     
REMARK   3   FREE R VALUE TEST SET SELECTION   : RANDOM                         
REMARK   3   R VALUE     (WORKING + TEST SET)  : 0.231                          
REMARK   3   R VALUE            (WORKING SET)  : 0.230                          
REMARK   3   FREE R VALUE                      : 0.236                          
REMARK   3   FREE R VALUE TEST SET SIZE   (%)  : 4.890                          
REMARK   3   FREE R VALUE TEST SET COUNT       : 615                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE   : NULL                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED               : 6                        
REMARK   3   BIN RESOLUTION RANGE HIGH   (ANGSTROMS) : 2.45                     
REMARK   3   BIN RESOLUTION RANGE LOW    (ANGSTROMS) : 2.68                     
REMARK   3   BIN COMPLETENESS (WORKING+TEST)     (%) : 98.21                    
REMARK   3   REFLECTIONS IN BIN (WORKING + TEST SET) : 2749                     
REMARK   3   BIN R VALUE        (WORKING + TEST SET) : 0.2471                   
REMARK   3   REFLECTIONS IN BIN        (WORKING SET) : 2613                     
REMARK   3   BIN R VALUE               (WORKING SET) : 0.2467                   
REMARK   3   BIN FREE R VALUE                        : 0.2569                   
REMARK   3   BIN FREE R VALUE TEST SET SIZE      (%) : 4.95                     
REMARK   3   BIN FREE R VALUE TEST SET COUNT         : 136                      
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE     : NULL                     
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1520                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 27                                      
REMARK   3   SOLVENT ATOMS            : 3                                       
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 57.02                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 93.55                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -18.78320                                            
REMARK   3    B22 (A**2) : -18.78320                                            
REMARK   3    B33 (A**2) : 37.56640                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT                    (A) : 0.609               
REMARK   3   DPI (BLOW EQ-10) BASED ON R VALUE        (A) : NULL                
REMARK   3   DPI (BLOW EQ-9) BASED ON FREE R VALUE    (A) : NULL                
REMARK   3   DPI (CRUICKSHANK) BASED ON R VALUE       (A) : 0.280               
REMARK   3   DPI (CRUICKSHANK) BASED ON FREE R VALUE  (A) : NULL                
REMARK   3                                                                      
REMARK   3   REFERENCES: BLOW, D. (2002) ACTA CRYST D58, 792-797                
REMARK   3               CRUICKSHANK, D.W.J. (1999) ACTA CRYST D55, 583-601     
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.895                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.923                         
REMARK   3                                                                      
REMARK   3   NUMBER OF GEOMETRIC FUNCTION TERMS DEFINED : 15                    
REMARK   3   TERM                          COUNT    WEIGHT   FUNCTION.          
REMARK   3    BOND LENGTHS              : 1585   ; 2.000  ; HARMONIC            
REMARK   3    BOND ANGLES               : 2141   ; 2.000  ; HARMONIC            
REMARK   3    TORSION ANGLES            : 546    ; 2.000  ; SINUSOIDAL          
REMARK   3    TRIGONAL CARBON PLANES    : 48     ; 2.000  ; HARMONIC            
REMARK   3    GENERAL PLANES            : 223    ; 5.000  ; HARMONIC            
REMARK   3    ISOTROPIC THERMAL FACTORS : 1585   ; 20.000 ; HARMONIC            
REMARK   3    BAD NON-BONDED CONTACTS   : 1      ; 5.000  ; SEMIHARMONIC        
REMARK   3    IMPROPER TORSIONS         : NULL   ; NULL   ; NULL                
REMARK   3    PSEUDOROTATION ANGLES     : NULL   ; NULL   ; NULL                
REMARK   3    CHIRAL IMPROPER TORSION   : 189    ; 5.000  ; SEMIHARMONIC        
REMARK   3    SUM OF OCCUPANCIES        : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY DISTANCES         : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY ANGLES            : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY TORSION           : NULL   ; NULL   ; NULL                
REMARK   3    IDEAL-DIST CONTACT TERM   : 1828   ; 4.000  ; SEMIHARMONIC        
REMARK   3                                                                      
REMARK   3   RMS DEVIATIONS FROM IDEAL VALUES.                                  
REMARK   3    BOND LENGTHS                       (A) : 0.010                    
REMARK   3    BOND ANGLES                  (DEGREES) : 1.20                     
REMARK   3    PEPTIDE OMEGA TORSION ANGLES (DEGREES) : 3.06                     
REMARK   3    OTHER TORSION ANGLES         (DEGREES) : 20.64                    
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 2                                          
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION:   A   77    A  170                                     
REMARK   3    ORIGIN FOR THE GROUP (A):   32.1889   30.9066   31.4518           
REMARK   3    T TENSOR                                                          
REMARK   3     T11:    0.0285 T22:    0.0570                                    
REMARK   3     T33:   -0.3258 T12:    0.4065                                    
REMARK   3     T13:   -0.0855 T23:   -0.1357                                    
REMARK   3    L TENSOR                                                          
REMARK   3     L11:    7.9631 L22:    2.7859                                    
REMARK   3     L33:   10.1475 L12:   -1.1089                                    
REMARK   3     L13:    4.2751 L23:   -0.7230                                    
REMARK   3    S TENSOR                                                          
REMARK   3     S11:    0.5694 S12:    0.3124 S13:   -0.4596                     
REMARK   3     S21:   -0.4720 S22:   -0.1050 S23:   -0.0733                     
REMARK   3     S31:    0.2566 S32:    1.3031 S33:   -0.4644                     
REMARK   3                                                                      
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION:   B   75    B  170                                     
REMARK   3    ORIGIN FOR THE GROUP (A):   17.1979   35.8835   12.3247           
REMARK   3    T TENSOR                                                          
REMARK   3     T11:    0.0614 T22:   -0.1690                                    
REMARK   3     T33:   -0.3023 T12:    0.2235                                    
REMARK   3     T13:   -0.1220 T23:   -0.0376                                    
REMARK   3    L TENSOR                                                          
REMARK   3     L11:    3.5256 L22:    3.3673                                    
REMARK   3     L33:   10.2803 L12:   -1.7563                                    
REMARK   3     L13:    1.7014 L23:   -0.6683                                    
REMARK   3    S TENSOR                                                          
REMARK   3     S11:    0.2827 S12:    0.6468 S13:   -0.3676                     
REMARK   3     S21:   -0.3454 S22:    0.0160 S23:    0.1888                     
REMARK   3     S31:    0.6457 S32:    1.0585 S33:   -0.2987                     
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4MHV COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 03-SEP-13.                  
REMARK 100 THE RCSB ID CODE IS RCSB081929.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 06-DEC-12                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : DIAMOND                            
REMARK 200  BEAMLINE                       : I04                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9795                             
REMARK 200  MONOCHROMATOR                  : SI 111 CHANNEL                     
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS                                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 12894                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.450                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 47.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 14.100                             
REMARK 200  R MERGE                    (I) : 0.10200                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 17.8000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.45                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.55                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 14.70                              
REMARK 200  R MERGE FOR SHELL          (I) : 0.01500                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.300                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MIR                          
REMARK 200 SOFTWARE USED: SHARP                                                 
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 64.56                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.47                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M CACADYLATE, PH 6.5, 14 % PEG       
REMARK 280  10K, 20 % GLYCEROL, 0.16 M CALCIUM ACETATE, VAPOR DIFFUSION,        
REMARK 280  HANGING DROP, TEMPERATURE 277K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 61 2 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+1/3                                            
REMARK 290       3555   -X+Y,-X,Z+2/3                                           
REMARK 290       4555   -X,-Y,Z+1/2                                             
REMARK 290       5555   Y,-X+Y,Z+5/6                                            
REMARK 290       6555   X-Y,X,Z+1/6                                             
REMARK 290       7555   Y,X,-Z+1/3                                              
REMARK 290       8555   X-Y,-Y,-Z                                               
REMARK 290       9555   -X,-X+Y,-Z+2/3                                          
REMARK 290      10555   -Y,-X,-Z+5/6                                            
REMARK 290      11555   -X+Y,Y,-Z+1/2                                           
REMARK 290      12555   X,X-Y,-Z+1/6                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       87.90333            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000      175.80667            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      131.85500            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000      219.75833            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       43.95167            
REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       87.90333            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000      175.80667            
REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000      219.75833            
REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000      131.85500            
REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000       43.95167            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A    74                                                      
REMARK 465     MET A    75                                                      
REMARK 465     LYS A    76                                                      
REMARK 465     SER B    74                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     VAL A  78    CG1  CG2                                            
REMARK 470     MET A  79    CG   SD   CE                                        
REMARK 470     GLN A  81    CG   CD   OE1  NE2                                  
REMARK 470     LEU A  83    CG   CD1  CD2                                       
REMARK 470     LYS A  84    CG   CD   CE   NZ                                   
REMARK 470     LYS A  91    CE   NZ                                             
REMARK 470     LYS A  92    CG   CD   CE   NZ                                   
REMARK 470     LYS A 101    CE   NZ                                             
REMARK 470     ARG A 131    CD   NE   CZ   NH1  NH2                             
REMARK 470     GLU A 145    CG   CD   OE1  OE2                                  
REMARK 470     MET B  75    CG   SD   CE                                        
REMARK 470     LYS B  92    CG   CD   CE   NZ                                   
REMARK 470     LYS B 101    CD   CE   NZ                                        
REMARK 470     GLU B 145    CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A 126       82.38   -154.13                                   
REMARK 500    PHE A 154      -43.36     68.08                                   
REMARK 500    LYS B  76       53.40   -101.33                                   
REMARK 500    PHE B 154      -45.73     76.59                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              CA A 201  CA                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH A 303   O                                                      
REMARK 620 2 HOH A 302   O   145.4                                              
REMARK 620 3 ILE A 167   O    73.6  78.8                                        
REMARK 620 4 ASN A 170   O   103.4 103.4 104.0                                  
REMARK 620 N                    1     2     3                                   
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CA A 201                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 202                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ACT A 203                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B 202                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ACT B 203                 
DBREF  4MHV A   76   170  UNP    P15036   ETS2_HUMAN      76    170             
DBREF  4MHV B   76   170  UNP    P15036   ETS2_HUMAN      76    170             
SEQADV 4MHV SER A   74  UNP  P15036              EXPRESSION TAG                 
SEQADV 4MHV MET A   75  UNP  P15036              EXPRESSION TAG                 
SEQADV 4MHV SER B   74  UNP  P15036              EXPRESSION TAG                 
SEQADV 4MHV MET B   75  UNP  P15036              EXPRESSION TAG                 
SEQRES   1 A   97  SER MET LYS ALA VAL MET SER GLN ALA LEU LYS ALA THR          
SEQRES   2 A   97  PHE SER GLY PHE LYS LYS GLU GLN ARG ARG LEU GLY ILE          
SEQRES   3 A   97  PRO LYS ASN PRO TRP LEU TRP SER GLU GLN GLN VAL CYS          
SEQRES   4 A   97  GLN TRP LEU LEU TRP ALA THR ASN GLU PHE SER LEU VAL          
SEQRES   5 A   97  ASN VAL ASN LEU GLN ARG PHE GLY MET ASN GLY GLN MET          
SEQRES   6 A   97  LEU CYS ASN LEU GLY LYS GLU ARG PHE LEU GLU LEU ALA          
SEQRES   7 A   97  PRO ASP PHE VAL GLY ASP ILE LEU TRP GLU HIS LEU GLU          
SEQRES   8 A   97  GLN MET ILE LYS GLU ASN                                      
SEQRES   1 B   97  SER MET LYS ALA VAL MET SER GLN ALA LEU LYS ALA THR          
SEQRES   2 B   97  PHE SER GLY PHE LYS LYS GLU GLN ARG ARG LEU GLY ILE          
SEQRES   3 B   97  PRO LYS ASN PRO TRP LEU TRP SER GLU GLN GLN VAL CYS          
SEQRES   4 B   97  GLN TRP LEU LEU TRP ALA THR ASN GLU PHE SER LEU VAL          
SEQRES   5 B   97  ASN VAL ASN LEU GLN ARG PHE GLY MET ASN GLY GLN MET          
SEQRES   6 B   97  LEU CYS ASN LEU GLY LYS GLU ARG PHE LEU GLU LEU ALA          
SEQRES   7 B   97  PRO ASP PHE VAL GLY ASP ILE LEU TRP GLU HIS LEU GLU          
SEQRES   8 B   97  GLN MET ILE LYS GLU ASN                                      
HET     CA  A 201       1                                                       
HET    GOL  A 202       6                                                       
HET    ACT  A 203       4                                                       
HET    GOL  B 201       6                                                       
HET    GOL  B 202       6                                                       
HET    ACT  B 203       4                                                       
HETNAM      CA CALCIUM ION                                                      
HETNAM     GOL GLYCEROL                                                         
HETNAM     ACT ACETATE ION                                                      
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
FORMUL   3   CA    CA 2+                                                        
FORMUL   4  GOL    3(C3 H8 O3)                                                  
FORMUL   5  ACT    2(C2 H3 O2 1-)                                               
FORMUL   9  HOH   *3(H2 O)                                                      
HELIX    1   1 ALA A   77  ALA A   85  1                                   9    
HELIX    2   2 THR A   86  SER A   88  5                                   3    
HELIX    3   3 GLY A   89  LEU A   97  1                                   9    
HELIX    4   4 ASN A  102  TRP A  106  5                                   5    
HELIX    5   5 SER A  107  PHE A  122  1                                  16    
HELIX    6   6 ASN A  128  GLY A  133  5                                   6    
HELIX    7   7 ASN A  135  LEU A  142  1                                   8    
HELIX    8   8 GLY A  143  ALA A  151  1                                   9    
HELIX    9   9 PHE A  154  ASN A  170  1                                  17    
HELIX   10  10 ALA B   77  THR B   86  1                                  10    
HELIX   11  11 GLY B   89  LEU B   97  1                                   9    
HELIX   12  12 ASN B  102  TRP B  106  5                                   5    
HELIX   13  13 SER B  107  PHE B  122  1                                  16    
HELIX   14  14 ASN B  128  PHE B  132  5                                   5    
HELIX   15  15 ASN B  135  LEU B  142  1                                   8    
HELIX   16  16 LEU B  142  ALA B  151  1                                  10    
HELIX   17  17 PHE B  154  GLU B  169  1                                  16    
LINK        CA    CA A 201                 O   HOH A 303     1555   1555  2.12  
LINK        CA    CA A 201                 O   HOH A 302     1555   1555  2.41  
LINK         O   ILE A 167                CA    CA A 201     1555   1555  2.45  
LINK         O   ASN A 170                CA    CA A 201     1555   1555  2.60  
SITE     1 AC1  6 ILE A 167  ASN A 170  HOH A 302  HOH A 303                    
SITE     2 AC1  6 ILE B 167  ASN B 170                                          
SITE     1 AC2  4 SER A 123  ASP A 153  PHE A 154  ASP A 157                    
SITE     1 AC3  2 VAL A 127  VAL B 127                                          
SITE     1 AC4  3 GLU A 149  GLU B 108  GLY B 133                               
SITE     1 AC5  5 ARG A  96  GLU B  93  TRP B 117  ASN B 120                    
SITE     2 AC5  5 ACT B 203                                                     
SITE     1 AC6  5 GLU A  93  ARG A  96  TRP A 117  ARG B  96                    
SITE     2 AC6  5 GOL B 202                                                     
CRYST1   64.440   64.440  263.710  90.00  90.00 120.00 P 61 2 2     24          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.015518  0.008960  0.000000        0.00000                         
SCALE2      0.000000  0.017919  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.003792        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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