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Database: PDB
Entry: 4MJS
LinkDB: 4MJS
Original site: 4MJS 
HEADER    TRANSFERASE/PROTEIN BINDING             04-SEP-13   4MJS              
TITLE     CRYSTAL STRUCTURE OF A PB1 COMPLEX                                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN KINASE C ZETA TYPE;                                
COMPND   3 CHAIN: A, C, E, G, I, K, M, O, Q, S, U, W;                           
COMPND   4 FRAGMENT: PB1 DOMAIN, UNP RESIDUES 15-101;                           
COMPND   5 SYNONYM: NPKC-ZETA;                                                  
COMPND   6 EC: 2.7.11.13;                                                       
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: SEQUESTOSOME-1;                                            
COMPND  10 CHAIN: B, D, F, H, J, L, N, P, R, T, V, X;                           
COMPND  11 FRAGMENT: PB1 DOMAIN, UNP RESIDUES 3-102;                            
COMPND  12 SYNONYM: EBI3-ASSOCIATED PROTEIN OF 60 KDA, EBIAP, P60,              
COMPND  13 PHOSPHOTYROSINE-INDEPENDENT LIGAND FOR THE LCK SH2 DOMAIN OF 62 KDA, 
COMPND  14 UBIQUITIN-BINDING PROTEIN P62;                                       
COMPND  15 ENGINEERED: YES;                                                     
COMPND  16 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE   3 ORGANISM_COMMON: BROWN RAT,RAT,RATS;                                 
SOURCE   4 ORGANISM_TAXID: 10116;                                               
SOURCE   5 GENE: PKCZ, PRKCZ;                                                   
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PGEX4T2;                                  
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606;                                                
SOURCE  15 GENE: ORCA, OSIL, SQSTM1;                                            
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PACYCDUET                                 
KEYWDS    PB1 DOMAIN, PB1 HETERODIMER AND PROTEIN INTERACTION, TRANSFERASE-     
KEYWDS   2 PROTEIN BINDING COMPLEX                                              
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.REN,Z.X.WANG,J.W.WU                                                 
REVDAT   2   29-OCT-14 4MJS    1       JRNL                                     
REVDAT   1   27-AUG-14 4MJS    0                                                
JRNL        AUTH   J.REN,J.WANG,Z.X.WANG,J.W.WU                                 
JRNL        TITL   STRUCTURAL AND BIOCHEMICAL INSIGHTS INTO THE HOMOTYPIC       
JRNL        TITL 2 PB1-PB1 COMPLEX BETWEEN PKC ZETA AND P62                     
JRNL        REF    SCI CHINA LIFE SCI            V.  57    69 2014              
JRNL        REFN                   ISSN 1674-7305                               
JRNL        PMID   24369353                                                     
JRNL        DOI    10.1007/S11427-013-4592-Z                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.7.3_928)                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 39.53                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9                           
REMARK   3   NUMBER OF REFLECTIONS             : 105316                         
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.240                           
REMARK   3   R VALUE            (WORKING SET) : 0.238                           
REMARK   3   FREE R VALUE                     : 0.287                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 5264                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 39.5385 -  7.7492    0.98     3540   184  0.2323 0.2770        
REMARK   3     2  7.7492 -  6.1583    1.00     3424   189  0.2674 0.2649        
REMARK   3     3  6.1583 -  5.3820    1.00     3405   186  0.2721 0.3029        
REMARK   3     4  5.3820 -  4.8909    1.00     3395   183  0.2086 0.2392        
REMARK   3     5  4.8909 -  4.5409    1.00     3354   187  0.1867 0.2196        
REMARK   3     6  4.5409 -  4.2735    1.00     3367   169  0.1871 0.2451        
REMARK   3     7  4.2735 -  4.0597    1.00     3352   182  0.2050 0.2546        
REMARK   3     8  4.0597 -  3.8832    1.00     3342   173  0.2153 0.2636        
REMARK   3     9  3.8832 -  3.7338    1.00     3354   161  0.2171 0.2817        
REMARK   3    10  3.7338 -  3.6050    1.00     3348   172  0.2360 0.2533        
REMARK   3    11  3.6050 -  3.4924    1.00     3341   189  0.2529 0.3239        
REMARK   3    12  3.4924 -  3.3926    1.00     3288   168  0.2437 0.2868        
REMARK   3    13  3.3926 -  3.3033    1.00     3341   166  0.2489 0.3222        
REMARK   3    14  3.3033 -  3.2228    1.00     3354   164  0.2459 0.2784        
REMARK   3    15  3.2228 -  3.1495    1.00     3296   170  0.2572 0.3235        
REMARK   3    16  3.1495 -  3.0825    1.00     3320   159  0.2578 0.3449        
REMARK   3    17  3.0825 -  3.0209    1.00     3314   187  0.2587 0.3051        
REMARK   3    18  3.0209 -  2.9639    1.00     3308   164  0.2493 0.3287        
REMARK   3    19  2.9639 -  2.9110    1.00     3347   176  0.2609 0.3119        
REMARK   3    20  2.9110 -  2.8617    1.00     3273   187  0.2675 0.3369        
REMARK   3    21  2.8617 -  2.8155    1.00     3289   190  0.2703 0.3427        
REMARK   3    22  2.8155 -  2.7722    1.00     3297   148  0.2635 0.3007        
REMARK   3    23  2.7722 -  2.7315    1.00     3322   192  0.2807 0.3402        
REMARK   3    24  2.7315 -  2.6930    1.00     3319   150  0.2827 0.3658        
REMARK   3    25  2.6930 -  2.6566    1.00     3282   198  0.2721 0.3309        
REMARK   3    26  2.6566 -  2.6221    1.00     3294   181  0.2715 0.3557        
REMARK   3    27  2.6221 -  2.5893    1.00     3262   185  0.2621 0.3307        
REMARK   3    28  2.5893 -  2.5582    1.00     3334   173  0.2607 0.3489        
REMARK   3    29  2.5582 -  2.5284    1.00     3275   166  0.2657 0.3173        
REMARK   3    30  2.5284 -  2.5000    1.00     3315   165  0.2741 0.3813        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.20                                          
REMARK   3   SHRINKAGE RADIUS   : 0.98                                          
REMARK   3   K_SOL              : 0.31                                          
REMARK   3   B_SOL              : 30.28                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.370            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 29.450           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 1.15690                                              
REMARK   3    B22 (A**2) : -1.51230                                             
REMARK   3    B33 (A**2) : 4.06580                                              
REMARK   3    B12 (A**2) : -0.00000                                             
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : -0.00000                                             
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.009          16736                                  
REMARK   3   ANGLE     :  1.200          22511                                  
REMARK   3   CHIRALITY :  0.078           2499                                  
REMARK   3   PLANARITY :  0.005           2882                                  
REMARK   3   DIHEDRAL  : 17.137           6270                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4MJS COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 05-SEP-13.                  
REMARK 100 THE RCSB ID CODE IS RCSB081998.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 22-DEC-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 8.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRF                               
REMARK 200  BEAMLINE                       : BL17U                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97892                            
REMARK 200  MONOCHROMATOR                  : DOUBLE CRYSTAL                     
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 105401                             
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 40.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 5.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 7.300                              
REMARK 200  R MERGE                    (I) : 0.08600                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 22.6000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.59                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.50                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.51900                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 4.100                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1WMH                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 57.30                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.88                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M TRIS-HCL, 8% PEG8000, 0.4M MGCL2,   
REMARK 280  PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 294K             
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       42.85750            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      129.77950            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       67.84050            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000      129.77950            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       42.85750            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       67.84050            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12                   
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E, F                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 4                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: G, H                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 5                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: I, J                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 6                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: K, L                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 7                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: M, N                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 8                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: O, P                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 9                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: Q, R                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 10                                                      
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: S, T                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 11                                                      
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: U, V                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 12                                                      
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: W, X                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    11                                                      
REMARK 465     PRO A    33                                                      
REMARK 465     GLU A   101                                                      
REMARK 465     GLY B    -1                                                      
REMARK 465     PRO B     0                                                      
REMARK 465     HIS B     1                                                      
REMARK 465     MET B     2                                                      
REMARK 465     SER B     3                                                      
REMARK 465     GLY B    12                                                      
REMARK 465     LYS B    13                                                      
REMARK 465     GLU B    14                                                      
REMARK 465     CYS B    27                                                      
REMARK 465     SER B    28                                                      
REMARK 465     PRO B    29                                                      
REMARK 465     GLU B    30                                                      
REMARK 465     PRO B    31                                                      
REMARK 465     GLU B    32                                                      
REMARK 465     ALA B    33                                                      
REMARK 465     GLU B    34                                                      
REMARK 465     ALA B    35                                                      
REMARK 465     GLU B    36                                                      
REMARK 465     ALA B    37                                                      
REMARK 465     ALA B    38                                                      
REMARK 465     ALA B    39                                                      
REMARK 465     GLY B    40                                                      
REMARK 465     GLY C    11                                                      
REMARK 465     PRO C    33                                                      
REMARK 465     GLU C   101                                                      
REMARK 465     GLY D    -1                                                      
REMARK 465     PRO D     0                                                      
REMARK 465     HIS D     1                                                      
REMARK 465     MET D     2                                                      
REMARK 465     GLY D    12                                                      
REMARK 465     LYS D    13                                                      
REMARK 465     GLU D    14                                                      
REMARK 465     CYS D    27                                                      
REMARK 465     SER D    28                                                      
REMARK 465     PRO D    29                                                      
REMARK 465     GLU D    30                                                      
REMARK 465     PRO D    31                                                      
REMARK 465     GLU D    32                                                      
REMARK 465     ALA D    33                                                      
REMARK 465     GLU D    34                                                      
REMARK 465     ALA D    35                                                      
REMARK 465     GLU D    36                                                      
REMARK 465     ALA D    37                                                      
REMARK 465     ALA D    38                                                      
REMARK 465     ALA D    39                                                      
REMARK 465     GLY D    40                                                      
REMARK 465     GLY E    11                                                      
REMARK 465     PRO E    33                                                      
REMARK 465     GLU E   101                                                      
REMARK 465     GLY F    -1                                                      
REMARK 465     PRO F     0                                                      
REMARK 465     HIS F     1                                                      
REMARK 465     MET F     2                                                      
REMARK 465     GLY F    12                                                      
REMARK 465     LYS F    13                                                      
REMARK 465     GLU F    14                                                      
REMARK 465     CYS F    27                                                      
REMARK 465     SER F    28                                                      
REMARK 465     PRO F    29                                                      
REMARK 465     GLU F    30                                                      
REMARK 465     PRO F    31                                                      
REMARK 465     GLU F    32                                                      
REMARK 465     ALA F    33                                                      
REMARK 465     GLU F    34                                                      
REMARK 465     ALA F    35                                                      
REMARK 465     GLU F    36                                                      
REMARK 465     ALA F    37                                                      
REMARK 465     ALA F    38                                                      
REMARK 465     ALA F    39                                                      
REMARK 465     GLY F    40                                                      
REMARK 465     GLY G    11                                                      
REMARK 465     PRO G    33                                                      
REMARK 465     GLU G   101                                                      
REMARK 465     GLY H    -1                                                      
REMARK 465     PRO H     0                                                      
REMARK 465     HIS H     1                                                      
REMARK 465     MET H     2                                                      
REMARK 465     LYS H    13                                                      
REMARK 465     GLU H    14                                                      
REMARK 465     CYS H    27                                                      
REMARK 465     SER H    28                                                      
REMARK 465     PRO H    29                                                      
REMARK 465     GLU H    30                                                      
REMARK 465     PRO H    31                                                      
REMARK 465     GLU H    32                                                      
REMARK 465     ALA H    33                                                      
REMARK 465     GLU H    34                                                      
REMARK 465     ALA H    35                                                      
REMARK 465     GLU H    36                                                      
REMARK 465     ALA H    37                                                      
REMARK 465     ALA H    38                                                      
REMARK 465     ALA H    39                                                      
REMARK 465     GLY H    40                                                      
REMARK 465     GLY I    11                                                      
REMARK 465     PRO I    33                                                      
REMARK 465     GLU I   101                                                      
REMARK 465     GLY J    -1                                                      
REMARK 465     PRO J     0                                                      
REMARK 465     HIS J     1                                                      
REMARK 465     MET J     2                                                      
REMARK 465     SER J    28                                                      
REMARK 465     PRO J    29                                                      
REMARK 465     GLU J    30                                                      
REMARK 465     PRO J    31                                                      
REMARK 465     GLU J    32                                                      
REMARK 465     ALA J    33                                                      
REMARK 465     GLU J    34                                                      
REMARK 465     ALA J    35                                                      
REMARK 465     GLU J    36                                                      
REMARK 465     ALA J    37                                                      
REMARK 465     ALA J    38                                                      
REMARK 465     ALA J    39                                                      
REMARK 465     GLY J    40                                                      
REMARK 465     GLY K    11                                                      
REMARK 465     PRO K    33                                                      
REMARK 465     GLU K   101                                                      
REMARK 465     GLY L    -1                                                      
REMARK 465     PRO L     0                                                      
REMARK 465     HIS L     1                                                      
REMARK 465     MET L     2                                                      
REMARK 465     CYS L    27                                                      
REMARK 465     SER L    28                                                      
REMARK 465     PRO L    29                                                      
REMARK 465     GLU L    30                                                      
REMARK 465     PRO L    31                                                      
REMARK 465     GLU L    32                                                      
REMARK 465     ALA L    33                                                      
REMARK 465     GLU L    34                                                      
REMARK 465     ALA L    35                                                      
REMARK 465     GLU L    36                                                      
REMARK 465     ALA L    37                                                      
REMARK 465     ALA L    38                                                      
REMARK 465     ALA L    39                                                      
REMARK 465     GLY L    40                                                      
REMARK 465     GLY M    11                                                      
REMARK 465     PRO M    33                                                      
REMARK 465     GLU M   101                                                      
REMARK 465     GLY N    -1                                                      
REMARK 465     PRO N     0                                                      
REMARK 465     HIS N     1                                                      
REMARK 465     MET N     2                                                      
REMARK 465     GLY N    12                                                      
REMARK 465     LYS N    13                                                      
REMARK 465     GLU N    14                                                      
REMARK 465     CYS N    27                                                      
REMARK 465     SER N    28                                                      
REMARK 465     PRO N    29                                                      
REMARK 465     GLU N    30                                                      
REMARK 465     PRO N    31                                                      
REMARK 465     GLU N    32                                                      
REMARK 465     ALA N    33                                                      
REMARK 465     GLU N    34                                                      
REMARK 465     ALA N    35                                                      
REMARK 465     GLU N    36                                                      
REMARK 465     ALA N    37                                                      
REMARK 465     ALA N    38                                                      
REMARK 465     ALA N    39                                                      
REMARK 465     GLY N    40                                                      
REMARK 465     GLY O    11                                                      
REMARK 465     PRO O    33                                                      
REMARK 465     GLU O   101                                                      
REMARK 465     GLY P    -1                                                      
REMARK 465     PRO P     0                                                      
REMARK 465     HIS P     1                                                      
REMARK 465     MET P     2                                                      
REMARK 465     SER P     3                                                      
REMARK 465     GLY P    12                                                      
REMARK 465     LYS P    13                                                      
REMARK 465     GLU P    14                                                      
REMARK 465     CYS P    27                                                      
REMARK 465     SER P    28                                                      
REMARK 465     PRO P    29                                                      
REMARK 465     GLU P    30                                                      
REMARK 465     PRO P    31                                                      
REMARK 465     GLU P    32                                                      
REMARK 465     ALA P    33                                                      
REMARK 465     GLU P    34                                                      
REMARK 465     ALA P    35                                                      
REMARK 465     GLU P    36                                                      
REMARK 465     ALA P    37                                                      
REMARK 465     ALA P    38                                                      
REMARK 465     ALA P    39                                                      
REMARK 465     GLY P    40                                                      
REMARK 465     PRO P    41                                                      
REMARK 465     GLY Q    11                                                      
REMARK 465     PRO Q    33                                                      
REMARK 465     GLU Q   101                                                      
REMARK 465     GLY R    -1                                                      
REMARK 465     PRO R     0                                                      
REMARK 465     HIS R     1                                                      
REMARK 465     MET R     2                                                      
REMARK 465     LYS R    13                                                      
REMARK 465     GLU R    14                                                      
REMARK 465     CYS R    27                                                      
REMARK 465     SER R    28                                                      
REMARK 465     PRO R    29                                                      
REMARK 465     GLU R    30                                                      
REMARK 465     PRO R    31                                                      
REMARK 465     GLU R    32                                                      
REMARK 465     ALA R    33                                                      
REMARK 465     GLU R    34                                                      
REMARK 465     ALA R    35                                                      
REMARK 465     GLU R    36                                                      
REMARK 465     ALA R    37                                                      
REMARK 465     ALA R    38                                                      
REMARK 465     ALA R    39                                                      
REMARK 465     GLY R    40                                                      
REMARK 465     GLY S    11                                                      
REMARK 465     PRO S    33                                                      
REMARK 465     GLU S   101                                                      
REMARK 465     GLY T    -1                                                      
REMARK 465     PRO T     0                                                      
REMARK 465     HIS T     1                                                      
REMARK 465     MET T     2                                                      
REMARK 465     GLY T    12                                                      
REMARK 465     LYS T    13                                                      
REMARK 465     GLU T    14                                                      
REMARK 465     CYS T    27                                                      
REMARK 465     SER T    28                                                      
REMARK 465     PRO T    29                                                      
REMARK 465     GLU T    30                                                      
REMARK 465     PRO T    31                                                      
REMARK 465     GLU T    32                                                      
REMARK 465     ALA T    33                                                      
REMARK 465     GLU T    34                                                      
REMARK 465     ALA T    35                                                      
REMARK 465     GLU T    36                                                      
REMARK 465     ALA T    37                                                      
REMARK 465     ALA T    38                                                      
REMARK 465     ALA T    39                                                      
REMARK 465     GLY T    40                                                      
REMARK 465     GLY U    11                                                      
REMARK 465     PRO U    33                                                      
REMARK 465     GLU U   101                                                      
REMARK 465     GLY V    -1                                                      
REMARK 465     PRO V     0                                                      
REMARK 465     HIS V     1                                                      
REMARK 465     MET V     2                                                      
REMARK 465     SER V     3                                                      
REMARK 465     GLY V    12                                                      
REMARK 465     LYS V    13                                                      
REMARK 465     GLU V    14                                                      
REMARK 465     CYS V    26                                                      
REMARK 465     CYS V    27                                                      
REMARK 465     SER V    28                                                      
REMARK 465     PRO V    29                                                      
REMARK 465     GLU V    30                                                      
REMARK 465     PRO V    31                                                      
REMARK 465     GLU V    32                                                      
REMARK 465     ALA V    33                                                      
REMARK 465     GLU V    34                                                      
REMARK 465     ALA V    35                                                      
REMARK 465     GLU V    36                                                      
REMARK 465     ALA V    37                                                      
REMARK 465     ALA V    38                                                      
REMARK 465     ALA V    39                                                      
REMARK 465     GLY V    40                                                      
REMARK 465     LYS V   102                                                      
REMARK 465     GLY W    11                                                      
REMARK 465     PRO W    33                                                      
REMARK 465     GLU W   101                                                      
REMARK 465     GLY X    -1                                                      
REMARK 465     PRO X     0                                                      
REMARK 465     HIS X     1                                                      
REMARK 465     SER X    28                                                      
REMARK 465     PRO X    29                                                      
REMARK 465     GLU X    30                                                      
REMARK 465     PRO X    31                                                      
REMARK 465     GLU X    32                                                      
REMARK 465     ALA X    33                                                      
REMARK 465     GLU X    34                                                      
REMARK 465     ALA X    35                                                      
REMARK 465     GLU X    36                                                      
REMARK 465     ALA X    37                                                      
REMARK 465     ALA X    38                                                      
REMARK 465     ALA X    39                                                      
REMARK 465     GLY X    40                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ALA B  16     -179.78    -64.22                                   
REMARK 500    PHE B  25      126.35   -170.63                                   
REMARK 500    SER B  78        3.65   -153.93                                   
REMARK 500    ASP B  93        2.63     90.38                                   
REMARK 500    SER D  78       10.58   -142.37                                   
REMARK 500    LYS D  91      -64.84    -98.66                                   
REMARK 500    ASP D  93        7.02    -59.97                                   
REMARK 500    SER H  78       -0.27   -148.58                                   
REMARK 500    GLU J  14       98.53    -69.08                                   
REMARK 500    SER J  78       19.36   -147.78                                   
REMARK 500    TYR J  89      -62.87    -96.93                                   
REMARK 500    ASP J  93        6.57     81.22                                   
REMARK 500    ARG K  87       16.00     58.54                                   
REMARK 500    LYS L  13     -136.77     47.32                                   
REMARK 500    SER L  78       -1.15   -142.23                                   
REMARK 500    SER N  78       -2.19   -152.04                                   
REMARK 500    LYS N  91       51.15   -118.98                                   
REMARK 500    ALA P  16     -164.05   -102.07                                   
REMARK 500    TYR P  67     -156.36   -128.70                                   
REMARK 500    ALA P  69     -162.31   -103.95                                   
REMARK 500    ARG P  71       36.89    -78.65                                   
REMARK 500    MET P  87       11.33    -69.02                                   
REMARK 500    ASP P  93       -1.79     79.20                                   
REMARK 500    SER R  78       23.32   -153.80                                   
REMARK 500    ASP R  92       -9.94    -59.62                                   
REMARK 500    SER S  63       -5.85    -59.51                                   
REMARK 500    ARG S  87       69.06     38.94                                   
REMARK 500    SER U  71       27.78   -140.34                                   
REMARK 500    ARG U  87       71.88     45.25                                   
REMARK 500    SER V  78      -43.94   -149.90                                   
REMARK 500    SER V  88       30.13    -81.08                                   
REMARK 500    TYR V  89       16.71   -148.16                                   
REMARK 500    ASP V  93       44.15    -84.80                                   
REMARK 500    GLU X  14       66.43     61.85                                   
REMARK 500    GLU X  70      138.29    -37.98                                   
REMARK 500    SER X  78       20.37   -151.82                                   
REMARK 500    ILE X  94      115.37   -166.87                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO H 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO M 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO O 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE EDO X 201                 
DBREF  4MJS A   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS B    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS C   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS D    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS E   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS F    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS G   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS H    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS I   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS J    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS K   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS L    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS M   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS N    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS O   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS P    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS Q   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS R    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS S   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS T    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS U   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS V    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
DBREF  4MJS W   15   101  UNP    P09217   KPCZ_RAT        15    101             
DBREF  4MJS X    3   102  UNP    Q13501   SQSTM_HUMAN      3    102             
SEQADV 4MJS GLY A   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO A   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS A   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET A   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY B   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO B    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS B    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET B    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA B   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG B   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY C   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO C   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS C   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET C   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY D   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO D    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS D    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET D    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA D   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG D   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY E   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO E   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS E   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET E   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY F   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO F    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS F    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET F    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA F   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG F   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY G   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO G   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS G   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET G   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY H   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO H    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS H    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET H    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA H   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG H   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY I   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO I   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS I   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET I   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY J   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO J    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS J    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET J    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA J   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG J   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY K   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO K   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS K   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET K   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY L   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO L    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS L    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET L    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA L   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG L   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY M   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO M   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS M   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET M   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY N   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO N    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS N    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET N    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA N   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG N   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY O   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO O   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS O   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET O   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY P   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO P    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS P    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET P    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA P   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG P   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY Q   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO Q   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS Q   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET Q   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY R   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO R    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS R    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET R    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA R   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG R   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY S   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO S   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS S   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET S   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY T   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO T    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS T    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET T    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA T   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG T   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY U   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO U   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS U   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET U   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY V   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO V    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS V    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET V    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA V   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG V   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQADV 4MJS GLY W   11  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS PRO W   12  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS HIS W   13  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS MET W   14  UNP  P09217              EXPRESSION TAG                 
SEQADV 4MJS GLY X   -1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS PRO X    0  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS HIS X    1  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS MET X    2  UNP  Q13501              EXPRESSION TAG                 
SEQADV 4MJS ALA X   69  UNP  Q13501    ASP    69 ENGINEERED MUTATION            
SEQADV 4MJS ARG X   71  UNP  Q13501    ASP    71 ENGINEERED MUTATION            
SEQRES   1 A   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 A   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 A   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 A   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 A   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 A   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 A   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 B  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 B  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 B  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 B  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 B  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 B  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 B  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 B  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 C   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 C   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 C   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 C   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 C   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 C   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 C   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 D  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 D  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 D  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 D  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 D  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 D  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 D  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 D  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 E   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 E   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 E   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 E   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 E   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 E   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 E   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 F  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 F  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 F  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 F  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 F  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 F  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 F  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 F  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 G   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 G   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 G   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 G   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 G   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 G   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 G   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 H  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 H  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 H  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 H  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 H  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 H  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 H  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 H  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 I   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 I   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 I   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 I   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 I   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 I   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 I   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 J  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 J  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 J  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 J  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 J  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 J  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 J  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 J  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 K   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 K   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 K   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 K   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 K   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 K   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 K   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 L  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 L  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 L  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 L  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 L  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 L  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 L  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 L  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 M   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 M   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 M   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 M   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 M   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 M   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 M   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 N  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 N  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 N  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 N  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 N  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 N  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 N  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 N  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 O   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 O   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 O   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 O   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 O   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 O   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 O   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 P  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 P  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 P  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 P  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 P  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 P  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 P  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 P  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 Q   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 Q   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 Q   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 Q   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 Q   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 Q   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 Q   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 R  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 R  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 R  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 R  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 R  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 R  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 R  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 R  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 S   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 S   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 S   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 S   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 S   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 S   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 S   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 T  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 T  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 T  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 T  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 T  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 T  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 T  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 T  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 U   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 U   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 U   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 U   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 U   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 U   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 U   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 V  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 V  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 V  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 V  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 V  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 V  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 V  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 V  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
SEQRES   1 W   91  GLY PRO HIS MET ARG VAL ARG LEU LYS ALA HIS TYR GLY          
SEQRES   2 W   91  GLY ASP ILE LEU ILE THR SER VAL ASP PRO THR THR THR          
SEQRES   3 W   91  PHE GLN ASP LEU CYS GLU GLU VAL ARG ASP MET CYS GLY          
SEQRES   4 W   91  LEU HIS GLN GLN HIS PRO LEU THR LEU LYS TRP VAL ASP          
SEQRES   5 W   91  SER GLU GLY ASP PRO CYS THR VAL SER SER GLN MET GLU          
SEQRES   6 W   91  LEU GLU GLU ALA PHE ARG LEU ALA CYS GLN GLY ARG ASP          
SEQRES   7 W   91  GLU VAL LEU ILE ILE HIS VAL PHE PRO SER ILE PRO GLU          
SEQRES   1 X  104  GLY PRO HIS MET SER LEU THR VAL LYS ALA TYR LEU LEU          
SEQRES   2 X  104  GLY LYS GLU ASP ALA ALA ARG GLU ILE ARG ARG PHE SER          
SEQRES   3 X  104  PHE CYS CYS SER PRO GLU PRO GLU ALA GLU ALA GLU ALA          
SEQRES   4 X  104  ALA ALA GLY PRO GLY PRO CYS GLU ARG LEU LEU SER ARG          
SEQRES   5 X  104  VAL ALA ALA LEU PHE PRO ALA LEU ARG PRO GLY GLY PHE          
SEQRES   6 X  104  GLN ALA HIS TYR ARG ALA GLU ARG GLY ASP LEU VAL ALA          
SEQRES   7 X  104  PHE SER SER ASP GLU GLU LEU THR MET ALA MET SER TYR          
SEQRES   8 X  104  VAL LYS ASP ASP ILE PHE ARG ILE TYR ILE LYS GLU LYS          
HET    EDO  H 201       4                                                       
HET    EDO  M 201       4                                                       
HET    EDO  O 201       4                                                       
HET    EDO  X 201       4                                                       
HETNAM     EDO 1,2-ETHANEDIOL                                                   
HETSYN     EDO ETHYLENE GLYCOL                                                  
FORMUL  25  EDO    4(C2 H6 O2)                                                  
FORMUL  29  HOH   *397(H2 O)                                                    
HELIX    1   1 THR A   36  CYS A   48  1                                  13    
HELIX    2   2 SER A   72  GLY A   86  1                                  15    
HELIX    3   3 GLY B   42  PHE B   55  1                                  14    
HELIX    4   4 SER B   79  TYR B   89  1                                  11    
HELIX    5   5 THR C   36  GLY C   49  1                                  14    
HELIX    6   6 SER C   72  GLY C   86  1                                  15    
HELIX    7   7 GLY D   42  PHE D   55  1                                  14    
HELIX    8   8 SER D   79  SER D   88  1                                  10    
HELIX    9   9 THR E   36  CYS E   48  1                                  13    
HELIX   10  10 SER E   72  GLY E   86  1                                  15    
HELIX   11  11 GLY F   42  PHE F   55  1                                  14    
HELIX   12  12 SER F   79  MET F   87  1                                   9    
HELIX   13  13 SER F   88  VAL F   90  5                                   3    
HELIX   14  14 THR G   36  CYS G   48  1                                  13    
HELIX   15  15 SER G   72  GLY G   86  1                                  15    
HELIX   16  16 GLY H   42  PHE H   55  1                                  14    
HELIX   17  17 SER H   79  TYR H   89  1                                  11    
HELIX   18  18 THR I   36  GLY I   49  1                                  14    
HELIX   19  19 SER I   72  GLY I   86  1                                  15    
HELIX   20  20 GLY J   42  PHE J   55  1                                  14    
HELIX   21  21 SER J   79  VAL J   90  1                                  12    
HELIX   22  22 THR K   36  GLY K   49  1                                  14    
HELIX   23  23 SER K   72  GLY K   86  1                                  15    
HELIX   24  24 GLY L   42  PHE L   55  1                                  14    
HELIX   25  25 SER L   79  TYR L   89  1                                  11    
HELIX   26  26 THR M   36  GLY M   49  1                                  14    
HELIX   27  27 SER M   72  GLY M   86  1                                  15    
HELIX   28  28 GLY N   42  PHE N   55  1                                  14    
HELIX   29  29 SER N   79  TYR N   89  1                                  11    
HELIX   30  30 THR O   36  GLY O   49  1                                  14    
HELIX   31  31 SER O   72  GLY O   86  1                                  15    
HELIX   32  32 PRO P   43  PHE P   55  1                                  13    
HELIX   33  33 GLU P   81  MET P   87  1                                   7    
HELIX   34  34 THR Q   36  GLY Q   49  1                                  14    
HELIX   35  35 SER Q   72  GLY Q   86  1                                  15    
HELIX   36  36 GLY R   42  PHE R   55  1                                  14    
HELIX   37  37 SER R   79  SER R   88  1                                  10    
HELIX   38  38 THR S   36  GLY S   49  1                                  14    
HELIX   39  39 SER S   72  GLN S   85  1                                  14    
HELIX   40  40 GLY T   42  PHE T   55  1                                  14    
HELIX   41  41 SER T   79  SER T   88  1                                  10    
HELIX   42  42 THR U   36  CYS U   48  1                                  13    
HELIX   43  43 SER U   72  GLY U   86  1                                  15    
HELIX   44  44 GLY V   42  PHE V   55  1                                  14    
HELIX   45  45 SER V   79  MET V   87  1                                   9    
HELIX   46  46 THR W   36  GLY W   49  1                                  14    
HELIX   47  47 SER W   72  GLY W   86  1                                  15    
HELIX   48  48 GLY X   42  PHE X   55  1                                  14    
HELIX   49  49 SER X   79  TYR X   89  1                                  11    
SHEET    1   A 5 ASP A  25  VAL A  31  0                                        
SHEET    2   A 5 ARG A  15  TYR A  22 -1  N  LEU A  18   O  THR A  29           
SHEET    3   A 5 VAL A  90  PRO A  97  1  O  VAL A  95   N  HIS A  21           
SHEET    4   A 5 LEU A  56  VAL A  61 -1  N  LYS A  59   O  HIS A  94           
SHEET    5   A 5 PRO A  67  VAL A  70 -1  O  VAL A  70   N  LEU A  58           
SHEET    1   B 4 GLU B  19  SER B  24  0                                        
SHEET    2   B 4 THR B   5  LEU B  10 -1  N  LEU B  10   O  GLU B  19           
SHEET    3   B 4 PHE B  95  GLU B 101  1  O  ILE B  97   N  TYR B   9           
SHEET    4   B 4 PHE B  63  GLN B  64 -1  N  GLN B  64   O  LYS B 100           
SHEET    1   C 2 HIS B  66  ARG B  68  0                                        
SHEET    2   C 2 LEU B  74  ALA B  76 -1  O  VAL B  75   N  TYR B  67           
SHEET    1   D 5 ASP C  25  VAL C  31  0                                        
SHEET    2   D 5 ARG C  15  TYR C  22 -1  N  LEU C  18   O  THR C  29           
SHEET    3   D 5 VAL C  90  PRO C  97  1  O  VAL C  95   N  HIS C  21           
SHEET    4   D 5 LEU C  56  VAL C  61 -1  N  THR C  57   O  PHE C  96           
SHEET    5   D 5 PRO C  67  VAL C  70 -1  O  CYS C  68   N  TRP C  60           
SHEET    1   E 5 GLU D  19  PHE D  25  0                                        
SHEET    2   E 5 LEU D   4  LEU D  10 -1  N  LEU D   4   O  PHE D  25           
SHEET    3   E 5 ILE D  94  GLU D 101  1  O  ILE D  99   N  TYR D   9           
SHEET    4   E 5 PHE D  63  ARG D  68 -1  N  GLN D  64   O  LYS D 100           
SHEET    5   E 5 LEU D  74  ALA D  76 -1  O  VAL D  75   N  TYR D  67           
SHEET    1   F 5 ASP E  25  VAL E  31  0                                        
SHEET    2   F 5 VAL E  16  TYR E  22 -1  N  LEU E  18   O  THR E  29           
SHEET    3   F 5 LEU E  91  PRO E  97  1  O  ILE E  93   N  LYS E  19           
SHEET    4   F 5 LEU E  56  VAL E  61 -1  N  LYS E  59   O  HIS E  94           
SHEET    5   F 5 PRO E  67  THR E  69 -1  O  CYS E  68   N  TRP E  60           
SHEET    1   G 5 GLU F  19  PHE F  25  0                                        
SHEET    2   G 5 LEU F   4  LEU F  10 -1  N  LEU F   4   O  PHE F  25           
SHEET    3   G 5 PHE F  95  GLU F 101  1  O  ILE F  99   N  TYR F   9           
SHEET    4   G 5 PHE F  63  ARG F  68 -1  N  GLN F  64   O  LYS F 100           
SHEET    5   G 5 LEU F  74  ALA F  76 -1  O  VAL F  75   N  TYR F  67           
SHEET    1   H 5 ASP G  25  VAL G  31  0                                        
SHEET    2   H 5 VAL G  16  TYR G  22 -1  N  LEU G  18   O  THR G  29           
SHEET    3   H 5 LEU G  91  PRO G  97  1  O  VAL G  95   N  HIS G  21           
SHEET    4   H 5 LEU G  56  VAL G  61 -1  N  THR G  57   O  PHE G  96           
SHEET    5   H 5 PRO G  67  VAL G  70 -1  O  CYS G  68   N  TRP G  60           
SHEET    1   I 5 ALA H  17  PHE H  25  0                                        
SHEET    2   I 5 LEU H   4  LEU H  11 -1  N  LEU H  10   O  ARG H  18           
SHEET    3   I 5 PHE H  95  GLU H 101  1  O  PHE H  95   N  THR H   5           
SHEET    4   I 5 PHE H  63  ARG H  68 -1  N  GLN H  64   O  LYS H 100           
SHEET    5   I 5 LEU H  74  PHE H  77 -1  O  VAL H  75   N  TYR H  67           
SHEET    1   J 5 ASP I  25  VAL I  31  0                                        
SHEET    2   J 5 ARG I  15  TYR I  22 -1  N  ALA I  20   O  LEU I  27           
SHEET    3   J 5 VAL I  90  PRO I  97  1  O  ILE I  93   N  LYS I  19           
SHEET    4   J 5 LEU I  56  VAL I  61 -1  N  THR I  57   O  PHE I  96           
SHEET    5   J 5 PRO I  67  VAL I  70 -1  O  CYS I  68   N  TRP I  60           
SHEET    1   K 5 ALA J  16  PHE J  25  0                                        
SHEET    2   K 5 LEU J   4  LYS J  13 -1  N  LEU J   4   O  PHE J  25           
SHEET    3   K 5 PHE J  95  GLU J 101  1  O  PHE J  95   N  THR J   5           
SHEET    4   K 5 PHE J  63  ARG J  68 -1  N  GLN J  64   O  LYS J 100           
SHEET    5   K 5 LEU J  74  PHE J  77 -1  O  VAL J  75   N  TYR J  67           
SHEET    1   L 5 ASP K  25  VAL K  31  0                                        
SHEET    2   L 5 ARG K  15  TYR K  22 -1  N  ALA K  20   O  LEU K  27           
SHEET    3   L 5 VAL K  90  PRO K  97  1  O  LEU K  91   N  ARG K  15           
SHEET    4   L 5 LEU K  56  VAL K  61 -1  N  THR K  57   O  PHE K  96           
SHEET    5   L 5 PRO K  67  VAL K  70 -1  O  CYS K  68   N  TRP K  60           
SHEET    1   M 5 ALA L  16  PHE L  25  0                                        
SHEET    2   M 5 LEU L   4  GLY L  12 -1  N  GLY L  12   O  ALA L  16           
SHEET    3   M 5 ILE L  94  GLU L 101  1  O  ILE L  99   N  TYR L   9           
SHEET    4   M 5 PHE L  63  ARG L  68 -1  N  HIS L  66   O  TYR L  98           
SHEET    5   M 5 LEU L  74  PHE L  77 -1  O  PHE L  77   N  ALA L  65           
SHEET    1   N 5 ASP M  25  VAL M  31  0                                        
SHEET    2   N 5 ARG M  15  TYR M  22 -1  N  ALA M  20   O  LEU M  27           
SHEET    3   N 5 VAL M  90  PRO M  97  1  O  ILE M  93   N  ARG M  17           
SHEET    4   N 5 LEU M  56  VAL M  61 -1  N  THR M  57   O  PHE M  96           
SHEET    5   N 5 PRO M  67  THR M  69 -1  O  CYS M  68   N  TRP M  60           
SHEET    1   O 5 GLU N  19  PHE N  25  0                                        
SHEET    2   O 5 LEU N   4  LEU N  10 -1  N  LEU N   4   O  PHE N  25           
SHEET    3   O 5 PHE N  95  GLU N 101  1  O  PHE N  95   N  THR N   5           
SHEET    4   O 5 PHE N  63  ARG N  68 -1  N  GLN N  64   O  LYS N 100           
SHEET    5   O 5 LEU N  74  ALA N  76 -1  O  VAL N  75   N  TYR N  67           
SHEET    1   P 5 ASP O  25  VAL O  31  0                                        
SHEET    2   P 5 VAL O  16  TYR O  22 -1  N  VAL O  16   O  VAL O  31           
SHEET    3   P 5 LEU O  91  PRO O  97  1  O  ILE O  93   N  ARG O  17           
SHEET    4   P 5 LEU O  56  VAL O  61 -1  N  LYS O  59   O  HIS O  94           
SHEET    5   P 5 PRO O  67  THR O  69 -1  O  CYS O  68   N  TRP O  60           
SHEET    1   Q 5 GLU P  19  SER P  24  0                                        
SHEET    2   Q 5 THR P   5  LEU P  10 -1  N  LEU P  10   O  GLU P  19           
SHEET    3   Q 5 PHE P  95  GLU P 101  1  O  ILE P  97   N  TYR P   9           
SHEET    4   Q 5 PHE P  63  HIS P  66 -1  N  HIS P  66   O  TYR P  98           
SHEET    5   Q 5 ALA P  76  PHE P  77 -1  O  PHE P  77   N  ALA P  65           
SHEET    1   R 5 ASP Q  25  VAL Q  31  0                                        
SHEET    2   R 5 ARG Q  15  TYR Q  22 -1  N  VAL Q  16   O  VAL Q  31           
SHEET    3   R 5 VAL Q  90  PRO Q  97  1  O  LEU Q  91   N  ARG Q  15           
SHEET    4   R 5 LEU Q  56  VAL Q  61 -1  N  LYS Q  59   O  HIS Q  94           
SHEET    5   R 5 PRO Q  67  THR Q  69 -1  O  CYS Q  68   N  TRP Q  60           
SHEET    1   S 5 ALA R  17  PHE R  25  0                                        
SHEET    2   S 5 LEU R   4  LEU R  11 -1  N  LEU R  10   O  ARG R  18           
SHEET    3   S 5 ILE R  94  GLU R 101  1  O  ILE R  99   N  TYR R   9           
SHEET    4   S 5 PHE R  63  ARG R  68 -1  N  GLN R  64   O  LYS R 100           
SHEET    5   S 5 LEU R  74  ALA R  76 -1  O  VAL R  75   N  TYR R  67           
SHEET    1   T 5 ASP S  25  VAL S  31  0                                        
SHEET    2   T 5 ARG S  15  TYR S  22 -1  N  VAL S  16   O  VAL S  31           
SHEET    3   T 5 VAL S  90  PRO S  97  1  O  LEU S  91   N  ARG S  15           
SHEET    4   T 5 LEU S  56  VAL S  61 -1  N  LYS S  59   O  HIS S  94           
SHEET    5   T 5 PRO S  67  VAL S  70 -1  O  CYS S  68   N  TRP S  60           
SHEET    1   U 5 GLU T  19  PHE T  25  0                                        
SHEET    2   U 5 LEU T   4  LEU T  10 -1  N  LEU T  10   O  GLU T  19           
SHEET    3   U 5 PHE T  95  GLU T 101  1  O  ILE T  99   N  TYR T   9           
SHEET    4   U 5 PHE T  63  ARG T  68 -1  N  GLN T  64   O  LYS T 100           
SHEET    5   U 5 LEU T  74  ALA T  76 -1  O  VAL T  75   N  TYR T  67           
SHEET    1   V 5 ASP U  25  VAL U  31  0                                        
SHEET    2   V 5 ARG U  15  TYR U  22 -1  N  VAL U  16   O  VAL U  31           
SHEET    3   V 5 VAL U  90  PRO U  97  1  O  VAL U  95   N  LYS U  19           
SHEET    4   V 5 LEU U  56  VAL U  61 -1  N  LYS U  59   O  HIS U  94           
SHEET    5   V 5 PRO U  67  VAL U  70 -1  O  CYS U  68   N  TRP U  60           
SHEET    1   W 5 GLU V  19  SER V  24  0                                        
SHEET    2   W 5 THR V   5  LEU V  10 -1  N  ALA V   8   O  ARG V  21           
SHEET    3   W 5 PHE V  95  LYS V 100  1  O  ILE V  97   N  TYR V   9           
SHEET    4   W 5 GLN V  64  ALA V  69 -1  N  GLN V  64   O  LYS V 100           
SHEET    5   W 5 ASP V  73  ALA V  76 -1  O  ASP V  73   N  ALA V  69           
SHEET    1   X 5 ASP W  25  VAL W  31  0                                        
SHEET    2   X 5 ARG W  15  TYR W  22 -1  N  LEU W  18   O  THR W  29           
SHEET    3   X 5 VAL W  90  PRO W  97  1  O  VAL W  95   N  HIS W  21           
SHEET    4   X 5 LEU W  56  VAL W  61 -1  N  LYS W  59   O  HIS W  94           
SHEET    5   X 5 PRO W  67  THR W  69 -1  O  CYS W  68   N  TRP W  60           
SHEET    1   Y 5 ALA X  16  CYS X  26  0                                        
SHEET    2   Y 5 SER X   3  GLY X  12 -1  N  LEU X   4   O  PHE X  25           
SHEET    3   Y 5 PHE X  95  GLU X 101  1  O  ILE X  99   N  TYR X   9           
SHEET    4   Y 5 PHE X  63  ALA X  69 -1  N  GLN X  64   O  LYS X 100           
SHEET    5   Y 5 ASP X  73  ALA X  76 -1  O  ASP X  73   N  ALA X  69           
SITE     1 AC1  6 TYR H  67  ARG H  68  TYR H  89  ILE H  94                    
SITE     2 AC1  6 ARG H  96  HOH H 311                                          
SITE     1 AC2  5 TRP M  60  VAL M  61  VAL M  90  LEU M  91                    
SITE     2 AC2  5 ILE M  92                                                     
SITE     1 AC3  3 PHE O  37  GLN O  38  GLN O  73                               
SITE     1 AC4  4 TYR X  67  ARG X  68  VAL X  90  ARG X  96                    
CRYST1   85.715  135.681  259.559  90.00  90.00  90.00 P 21 21 21   48          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.011667  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.007370  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.003853        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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