GenomeNet

Database: PDB
Entry: 4NEE
LinkDB: 4NEE
Original site: 4NEE 
HEADER    VIRAL PROTEIN/PROTEIN TRANSPORT         29-OCT-13   4NEE              
TITLE     CRYSTAL STRUCTURE OF AP-2 ALPHA/SIMGA2 COMPLEX BOUND TO HIV-1 NEF     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: AP-2 COMPLEX SUBUNIT SIGMA;                                
COMPND   3 CHAIN: D, L, F, I;                                                   
COMPND   4 SYNONYM: ADAPTER-RELATED PROTEIN COMPLEX 2 SUBUNIT SIGMA, ADAPTOR    
COMPND   5 PROTEIN COMPLEX AP-2 SUBUNIT SIGMA, CLATHRIN ASSEMBLY PROTEIN 2 SIGMA
COMPND   6 SMALL CHAIN, CLATHRIN COAT ASSEMBLY PROTEIN AP17, CLATHRIN COAT-     
COMPND   7 ASSOCIATED PROTEIN AP17, PLASMA MEMBRANE ADAPTOR AP-2 17 KDA PROTEIN,
COMPND   8 SIGMA-ADAPTIN 3B, SIGMA2-ADAPTIN;                                    
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MOL_ID: 2;                                                           
COMPND  11 MOLECULE: AP-2 COMPLEX SUBUNIT ALPHA-2;                              
COMPND  12 CHAIN: G, B, J, A;                                                   
COMPND  13 FRAGMENT: RESIDUES 1-396;                                            
COMPND  14 SYNONYM: ADAPTOR-RELATED PROTEIN COMPLEX 2, ALPHA 2 SUBUNIT;         
COMPND  15 ENGINEERED: YES;                                                     
COMPND  16 MOL_ID: 3;                                                           
COMPND  17 MOLECULE: PROTEIN NEF;                                               
COMPND  18 CHAIN: E, H, K, C;                                                   
COMPND  19 FRAGMENT: HIV-1 NEF, RESIDUES 54-203;                                
COMPND  20 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE   3 ORGANISM_COMMON: BROWN RAT,RAT,RATS;                                 
SOURCE   4 ORGANISM_TAXID: 10116;                                               
SOURCE   5 GENE: AP2S1, AP17, CLAPS2;                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE  10 ORGANISM_COMMON: BROWN RAT,RAT,RATS;                                 
SOURCE  11 ORGANISM_TAXID: 10116;                                               
SOURCE  12 GENE: AP17, AP2A2, AP2S1, CLAPS2;                                    
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  15 MOL_ID: 3;                                                           
SOURCE  16 ORGANISM_SCIENTIFIC: HUMAN IMMUNODEFICIENCY VIRUS 1;                 
SOURCE  17 ORGANISM_TAXID: 11676;                                               
SOURCE  18 GENE: NEF;                                                           
SOURCE  19 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  20 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    CLATHRIN ADAPTOR AP-2, HIV-1 NEF, CD4 DOWNREGULATION, VIRAL PROTEIN-  
KEYWDS   2 PROTEIN TRANSPORT COMPLEX                                            
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.H.HURLEY,J.S.BONIFACINO,X.REN,S.Y.PARK                              
REVDAT   2   05-MAR-14 4NEE    1       JRNL                                     
REVDAT   1   29-JAN-14 4NEE    0                                                
JRNL        AUTH   X.REN,S.Y.PARK,J.S.BONIFACINO,J.H.HURLEY                     
JRNL        TITL   HOW HIV-1 NEF HIJACKS THE AP-2 CLATHRIN ADAPTOR TO           
JRNL        TITL 2 DOWNREGULATE CD4.                                            
JRNL        REF    ELIFE                         V.   3 01754 2014              
JRNL        REFN                   ESSN 2050-084X                               
JRNL        PMID   24473078                                                     
JRNL        DOI    10.7554/ELIFE.01754                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.88 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8.3_1479)                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.88                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.88                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.420                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 95.5                           
REMARK   3   NUMBER OF REFLECTIONS             : 80121                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.222                           
REMARK   3   R VALUE            (WORKING SET) : 0.219                           
REMARK   3   FREE R VALUE                     : 0.267                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.010                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 4014                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 48.8868 -  8.8438    0.99     2920   164  0.1590 0.1905        
REMARK   3     2  8.8438 -  7.0263    1.00     2838   146  0.1678 0.1842        
REMARK   3     3  7.0263 -  6.1401    1.00     2799   136  0.1987 0.2297        
REMARK   3     4  6.1401 -  5.5795    1.00     2767   158  0.2027 0.2797        
REMARK   3     5  5.5795 -  5.1801    1.00     2805   120  0.2000 0.2390        
REMARK   3     6  5.1801 -  4.8750    1.00     2735   161  0.1825 0.2433        
REMARK   3     7  4.8750 -  4.6310    1.00     2771   135  0.1684 0.2120        
REMARK   3     8  4.6310 -  4.4296    1.00     2719   142  0.1694 0.1987        
REMARK   3     9  4.4296 -  4.2592    1.00     2745   146  0.1729 0.2363        
REMARK   3    10  4.2592 -  4.1123    1.00     2745   129  0.1733 0.2002        
REMARK   3    11  4.1123 -  3.9837    0.99     2720   141  0.1934 0.2495        
REMARK   3    12  3.9837 -  3.8699    0.98     2664   155  0.2281 0.2757        
REMARK   3    13  3.8699 -  3.7681    0.98     2675   140  0.2142 0.2410        
REMARK   3    14  3.7681 -  3.6762    0.98     2670   135  0.2191 0.3203        
REMARK   3    15  3.6762 -  3.5926    0.97     2650   140  0.2447 0.3269        
REMARK   3    16  3.5926 -  3.5162    0.98     2673   135  0.2356 0.2966        
REMARK   3    17  3.5162 -  3.4459    0.96     2608   145  0.2472 0.2854        
REMARK   3    18  3.4459 -  3.3809    0.96     2637   153  0.2511 0.2982        
REMARK   3    19  3.3809 -  3.3205    0.97     2625   137  0.2582 0.3350        
REMARK   3    20  3.3205 -  3.2642    0.97     2648   123  0.2748 0.3223        
REMARK   3    21  3.2642 -  3.2116    0.96     2584   157  0.2717 0.3148        
REMARK   3    22  3.2116 -  3.1622    0.96     2624   138  0.2876 0.3185        
REMARK   3    23  3.1622 -  3.1157    0.95     2551   135  0.2847 0.3425        
REMARK   3    24  3.1157 -  3.0718    0.94     2565   130  0.2943 0.3315        
REMARK   3    25  3.0718 -  3.0303    0.93     2525   137  0.3045 0.3545        
REMARK   3    26  3.0303 -  2.9910    0.94     2542   147  0.3115 0.3484        
REMARK   3    27  2.9910 -  2.9536    0.91     2466   126  0.3257 0.3583        
REMARK   3    28  2.9536 -  2.9180    0.87     2394   122  0.3382 0.4029        
REMARK   3    29  2.9180 -  2.8841    0.54     1442    81  0.3520 0.3936        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.400            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 25.120           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.015          21985                                  
REMARK   3   ANGLE     :  1.159          29768                                  
REMARK   3   CHIRALITY :  0.069           3364                                  
REMARK   3   PLANARITY :  0.007           3802                                  
REMARK   3   DIHEDRAL  : 14.286           8144                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 57                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: chain 'D' and (resid 1 through 41 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):   2.8538 -14.9206 -18.4448              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2453 T22:   0.3791                                     
REMARK   3      T33:   0.4155 T12:   0.0240                                     
REMARK   3      T13:  -0.0111 T23:  -0.0270                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.0113 L22:   4.0934                                     
REMARK   3      L33:   2.3199 L12:   0.2262                                     
REMARK   3      L13:  -0.8459 L23:   1.5356                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0685 S12:  -0.1907 S13:   0.2221                       
REMARK   3      S21:   0.1415 S22:  -0.3710 S23:   1.1883                       
REMARK   3      S31:   0.3379 S32:  -0.4585 S33:   0.2970                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: chain 'D' and (resid 42 through 48 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  13.9726 -23.0327  -5.2696              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8137 T22:   0.4076                                     
REMARK   3      T33:   0.7338 T12:   0.2433                                     
REMARK   3      T13:   0.1782 T23:   0.0674                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.9964 L22:   0.9624                                     
REMARK   3      L33:   8.0786 L12:  -2.4738                                     
REMARK   3      L13:  -2.6102 L23:  -1.0176                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1777 S12:  -1.3241 S13:   1.5019                       
REMARK   3      S21:   1.8348 S22:   1.1513 S23:  -0.6965                       
REMARK   3      S31:  -1.1194 S32:  -0.3726 S33:  -0.6187                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: chain 'D' and (resid 49 through 62 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   9.4755 -23.3410 -14.7626              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2180 T22:   0.5823                                     
REMARK   3      T33:   0.1248 T12:  -0.0266                                     
REMARK   3      T13:  -0.0985 T23:   0.1667                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.8973 L22:   4.6223                                     
REMARK   3      L33:   7.4576 L12:  -1.7433                                     
REMARK   3      L13:   2.3690 L23:   2.1568                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2885 S12:  -0.7787 S13:  -1.0856                       
REMARK   3      S21:   0.4366 S22:  -0.6585 S23:   0.7010                       
REMARK   3      S31:   1.3823 S32:  -0.5640 S33:  -0.6642                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: chain 'D' and (resid 63 through 142 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  17.5041 -11.4096 -25.8369              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1529 T22:   0.3227                                     
REMARK   3      T33:   0.2484 T12:   0.0290                                     
REMARK   3      T13:  -0.0484 T23:  -0.0031                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.8046 L22:   2.6689                                     
REMARK   3      L33:   2.4813 L12:  -1.3131                                     
REMARK   3      L13:  -0.1860 L23:  -0.0475                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0250 S12:   0.1875 S13:   0.2847                       
REMARK   3      S21:  -0.1267 S22:  -0.1145 S23:  -0.1341                       
REMARK   3      S31:  -0.1471 S32:   0.1140 S33:   0.1567                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: chain 'L' and (resid 1 through 41 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  36.6017   8.1750  29.2029              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5645 T22:   0.5327                                     
REMARK   3      T33:   0.3286 T12:  -0.1173                                     
REMARK   3      T13:   0.0863 T23:  -0.0555                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.6740 L22:   2.8106                                     
REMARK   3      L33:   0.1405 L12:   0.8324                                     
REMARK   3      L13:  -0.4144 L23:  -0.2613                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4187 S12:  -1.1661 S13:   0.3181                       
REMARK   3      S21:   1.3653 S22:  -0.4324 S23:   0.3212                       
REMARK   3      S31:   0.2909 S32:  -0.3591 S33:  -0.1174                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: chain 'L' and (resid 42 through 48 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  24.3501  15.6840  16.6826              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7804 T22:   0.8873                                     
REMARK   3      T33:   0.8332 T12:  -0.1535                                     
REMARK   3      T13:  -0.2584 T23:   0.0411                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.9118 L22:   5.6771                                     
REMARK   3      L33:   4.0119 L12:   0.5568                                     
REMARK   3      L13:  -2.3955 L23:  -3.8279                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5658 S12:  -0.5297 S13:  -1.0112                       
REMARK   3      S21:  -0.6082 S22:  -0.5911 S23:   1.3753                       
REMARK   3      S31:   1.5590 S32:  -2.0137 S33:  -0.1809                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: chain 'L' and (resid 49 through 55 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  30.9292  20.9675  28.4661              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4127 T22:   0.6648                                     
REMARK   3      T33:   0.8217 T12:  -0.0058                                     
REMARK   3      T13:   0.1450 T23:  -0.0480                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.2708 L22:   2.0396                                     
REMARK   3      L33:   2.4696 L12:  -2.6949                                     
REMARK   3      L13:  -0.1220 L23:   0.1801                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1243 S12:  -1.1406 S13:   0.1107                       
REMARK   3      S21:   0.6589 S22:   0.4356 S23:   0.4865                       
REMARK   3      S31:  -0.0680 S32:  -0.7791 S33:  -0.1941                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: chain 'L' and (resid 56 through 127 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  43.3755  10.2320  17.4226              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1724 T22:   0.3243                                     
REMARK   3      T33:   0.2402 T12:  -0.0226                                     
REMARK   3      T13:  -0.0018 T23:  -0.0228                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.2260 L22:   4.8925                                     
REMARK   3      L33:   1.8112 L12:  -1.2171                                     
REMARK   3      L13:   0.9061 L23:  -0.4400                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1620 S12:   0.1624 S13:   0.1578                       
REMARK   3      S21:  -0.0369 S22:  -0.1400 S23:   0.1227                       
REMARK   3      S31:   0.1899 S32:   0.0452 S33:  -0.0852                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: chain 'L' and (resid 128 through 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  58.8782   6.0991  12.3657              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2673 T22:   0.4964                                     
REMARK   3      T33:   0.3023 T12:   0.0941                                     
REMARK   3      T13:   0.0344 T23:   0.0029                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.7162 L22:   9.0867                                     
REMARK   3      L33:   6.3696 L12:  -4.5024                                     
REMARK   3      L13:   2.4680 L23:  -2.0034                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0885 S12:  -0.1569 S13:   0.1867                       
REMARK   3      S21:  -0.3583 S22:   0.0008 S23:  -0.9044                       
REMARK   3      S31:   0.4724 S32:   0.7516 S33:   0.0337                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: chain 'F' and (resid 1 through 41 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  73.9617 -55.9119 -33.2853              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3608 T22:   0.4495                                     
REMARK   3      T33:   0.6621 T12:   0.0716                                     
REMARK   3      T13:   0.1285 T23:   0.0694                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.8651 L22:   5.3391                                     
REMARK   3      L33:   1.4461 L12:   1.0930                                     
REMARK   3      L13:   0.7110 L23:  -1.5512                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2634 S12:   0.3301 S13:  -0.2681                       
REMARK   3      S21:   0.1986 S22:   0.2115 S23:  -1.7189                       
REMARK   3      S31:   0.0921 S32:   0.3305 S33:   0.1032                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: chain 'F' and (resid 42 through 48 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  64.7916 -55.3883 -16.6500              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3877 T22:   0.5837                                     
REMARK   3      T33:   1.2215 T12:   0.7573                                     
REMARK   3      T13:   0.2948 T23:   0.2821                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.1432 L22:   3.8380                                     
REMARK   3      L33:   1.6010 L12:  -2.8689                                     
REMARK   3      L13:   1.4746 L23:  -1.8767                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2311 S12:  -0.4631 S13:  -0.0908                       
REMARK   3      S21:  -0.1558 S22:  -0.5289 S23:  -0.2841                       
REMARK   3      S31:   0.3036 S32:   0.2904 S33:   0.4127                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: chain 'F' and (resid 49 through 62 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  67.9196 -50.4866 -25.0372              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4902 T22:   0.4537                                     
REMARK   3      T33:   0.4146 T12:   0.0530                                     
REMARK   3      T13:   0.0307 T23:   0.0973                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7797 L22:   1.0906                                     
REMARK   3      L33:   5.5097 L12:   0.6143                                     
REMARK   3      L13:  -2.6829 L23:  -1.7931                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2954 S12:  -0.6642 S13:   0.3208                       
REMARK   3      S21:   0.0576 S22:  -0.4732 S23:  -0.2408                       
REMARK   3      S31:  -0.3836 S32:   0.7666 S33:   0.1088                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: chain 'F' and (resid 63 through 127 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  60.0372 -55.9392 -37.1760              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4226 T22:   0.2535                                     
REMARK   3      T33:   0.3923 T12:   0.0051                                     
REMARK   3      T13:   0.1192 T23:   0.0940                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.6848 L22:   1.1119                                     
REMARK   3      L33:   2.5840 L12:   0.1289                                     
REMARK   3      L13:  -0.6980 L23:   0.6710                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2387 S12:   0.0464 S13:  -0.0340                       
REMARK   3      S21:  -0.2435 S22:   0.0332 S23:  -0.3762                       
REMARK   3      S31:   0.1862 S32:  -0.2272 S33:   0.0845                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: chain 'F' and (resid 128 through 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  51.4018 -57.3767 -50.1832              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5299 T22:   0.4078                                     
REMARK   3      T33:   0.4990 T12:  -0.0811                                     
REMARK   3      T13:   0.1151 T23:   0.0207                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.7586 L22:   4.4259                                     
REMARK   3      L33:   5.3325 L12:  -2.9711                                     
REMARK   3      L13:  -4.8214 L23:   4.0571                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5443 S12:   0.8693 S13:  -0.3451                       
REMARK   3      S21:  -0.3418 S22:  -0.1633 S23:  -0.5782                       
REMARK   3      S31:   0.8953 S32:  -0.6710 S33:   0.7065                       
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    SELECTION: chain 'I' and (resid 1 through 24 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -11.7061  15.6720  -1.0945              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2832 T22:   0.3830                                     
REMARK   3      T33:   0.2257 T12:  -0.0078                                     
REMARK   3      T13:  -0.0188 T23:   0.0278                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.1719 L22:   6.3778                                     
REMARK   3      L33:   2.1473 L12:  -0.0861                                     
REMARK   3      L13:  -0.3512 L23:   0.7742                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1094 S12:   0.7275 S13:   0.2599                       
REMARK   3      S21:  -0.9861 S22:  -0.0657 S23:  -0.0678                       
REMARK   3      S31:  -0.2601 S32:   0.4600 S33:   0.0971                       
REMARK   3   TLS GROUP : 16                                                     
REMARK   3    SELECTION: chain 'I' and (resid 25 through 41 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.3373  15.9621  -3.7251              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3316 T22:   0.4340                                     
REMARK   3      T33:   0.2571 T12:   0.0189                                     
REMARK   3      T13:   0.0755 T23:  -0.0199                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.3961 L22:   8.7821                                     
REMARK   3      L33:   3.5838 L12:  -2.4799                                     
REMARK   3      L13:   1.6032 L23:  -2.7943                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2149 S12:   0.2732 S13:   0.4177                       
REMARK   3      S21:  -1.4007 S22:  -0.0614 S23:  -0.2889                       
REMARK   3      S31:   0.3306 S32:   0.7418 S33:  -0.2820                       
REMARK   3   TLS GROUP : 17                                                     
REMARK   3    SELECTION: chain 'I' and (resid 42 through 54 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -0.1894  20.5296   9.3890              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4194 T22:   0.6634                                     
REMARK   3      T33:   0.4274 T12:   0.0949                                     
REMARK   3      T13:   0.0794 T23:  -0.0539                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.0752 L22:   5.2789                                     
REMARK   3      L33:   5.3434 L12:   2.7535                                     
REMARK   3      L13:  -1.7341 L23:   1.1179                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1168 S12:   0.9550 S13:   0.4926                       
REMARK   3      S21:  -0.5414 S22:  -0.0523 S23:  -1.3806                       
REMARK   3      S31:   1.1563 S32:   1.1574 S33:  -0.3995                       
REMARK   3   TLS GROUP : 18                                                     
REMARK   3    SELECTION: chain 'I' and (resid 55 through 127 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -16.5577  13.1216   8.4241              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1545 T22:   0.2380                                     
REMARK   3      T33:   0.2178 T12:  -0.0029                                     
REMARK   3      T13:   0.0072 T23:   0.0422                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7909 L22:   1.9885                                     
REMARK   3      L33:   2.3761 L12:   0.2554                                     
REMARK   3      L13:   0.1978 L23:   1.2804                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0278 S12:   0.0088 S13:   0.0619                       
REMARK   3      S21:  -0.0759 S22:   0.0732 S23:  -0.0413                       
REMARK   3      S31:   0.0801 S32:  -0.0598 S33:  -0.0891                       
REMARK   3   TLS GROUP : 19                                                     
REMARK   3    SELECTION: chain 'I' and (resid 128 through 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -32.9473   8.0535   9.2779              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1819 T22:   0.3513                                     
REMARK   3      T33:   0.3248 T12:  -0.1451                                     
REMARK   3      T13:   0.0249 T23:   0.0144                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.9143 L22:   1.2478                                     
REMARK   3      L33:   7.1351 L12:  -1.2203                                     
REMARK   3      L13:   2.2840 L23:  -0.1429                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2237 S12:  -0.2185 S13:  -0.3156                       
REMARK   3      S21:   0.0098 S22:  -0.3673 S23:   0.7386                       
REMARK   3      S31:   1.4301 S32:  -0.5883 S33:   0.1469                       
REMARK   3   TLS GROUP : 20                                                     
REMARK   3    SELECTION: chain 'G' and (resid 9 through 140 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -26.0398   5.9710 -11.8048              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2696 T22:   0.3525                                     
REMARK   3      T33:   0.2589 T12:   0.0037                                     
REMARK   3      T13:  -0.0497 T23:   0.0119                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.0281 L22:   3.8955                                     
REMARK   3      L33:   1.6742 L12:  -2.1256                                     
REMARK   3      L13:  -0.2723 L23:   0.6635                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1387 S12:   0.4878 S13:  -0.0828                       
REMARK   3      S21:  -0.3911 S22:  -0.2375 S23:   0.2282                       
REMARK   3      S31:   0.0543 S32:  -0.1494 S33:   0.0879                       
REMARK   3   TLS GROUP : 21                                                     
REMARK   3    SELECTION: chain 'G' and (resid 141 through 219 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -31.2714  31.2027   2.0219              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2292 T22:   0.3598                                     
REMARK   3      T33:   0.4512 T12:   0.0656                                     
REMARK   3      T13:   0.0086 T23:  -0.0276                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.4986 L22:   1.1325                                     
REMARK   3      L33:   3.7451 L12:   0.4739                                     
REMARK   3      L13:   0.3484 L23:  -0.0252                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0360 S12:   0.1863 S13:   0.3590                       
REMARK   3      S21:  -0.0682 S22:  -0.0777 S23:   0.1870                       
REMARK   3      S31:  -0.3217 S32:  -0.4068 S33:   0.1067                       
REMARK   3   TLS GROUP : 22                                                     
REMARK   3    SELECTION: chain 'G' and (resid 220 through 358 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -16.6301  32.1840  22.9657              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2785 T22:   0.2991                                     
REMARK   3      T33:   0.3228 T12:  -0.0744                                     
REMARK   3      T13:   0.0329 T23:  -0.0676                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.1620 L22:   2.3651                                     
REMARK   3      L33:   2.1474 L12:  -0.5468                                     
REMARK   3      L13:   0.8788 L23:   0.3404                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0432 S12:  -0.3830 S13:   0.4832                       
REMARK   3      S21:   0.3894 S22:   0.0101 S23:   0.0255                       
REMARK   3      S31:  -0.1417 S32:  -0.0586 S33:  -0.0941                       
REMARK   3   TLS GROUP : 23                                                     
REMARK   3    SELECTION: chain 'G' and (resid 359 through 396 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   5.7349  34.7159  21.3868              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2975 T22:   0.4684                                     
REMARK   3      T33:   0.7463 T12:  -0.1076                                     
REMARK   3      T13:  -0.0538 T23:  -0.1074                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.8874 L22:   5.7626                                     
REMARK   3      L33:   4.1985 L12:   0.9998                                     
REMARK   3      L13:   0.0843 L23:   0.7835                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0116 S12:   0.0407 S13:   0.5495                       
REMARK   3      S21:  -0.0673 S22:  -0.0966 S23:  -0.0937                       
REMARK   3      S31:  -0.2211 S32:   0.2874 S33:   0.0644                       
REMARK   3   TLS GROUP : 24                                                     
REMARK   3    SELECTION: chain 'B' and (resid 9 through 87 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):   0.7007   6.9769 -28.5023              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3657 T22:   0.3328                                     
REMARK   3      T33:   0.3631 T12:   0.0827                                     
REMARK   3      T13:  -0.0288 T23:   0.0858                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.3814 L22:   2.8265                                     
REMARK   3      L33:   3.3354 L12:  -1.3336                                     
REMARK   3      L13:   0.5405 L23:   1.2692                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1915 S12:   0.0031 S13:   0.1587                       
REMARK   3      S21:  -0.3397 S22:  -0.1543 S23:   0.4188                       
REMARK   3      S31:  -0.6035 S32:  -0.4851 S33:  -0.0438                       
REMARK   3   TLS GROUP : 25                                                     
REMARK   3    SELECTION: chain 'B' and (resid 88 through 320 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  16.2357 -24.7416 -36.8434              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3201 T22:   0.3893                                     
REMARK   3      T33:   0.2285 T12:   0.0192                                     
REMARK   3      T13:  -0.0151 T23:  -0.0860                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.8897 L22:   4.0158                                     
REMARK   3      L33:   1.6321 L12:  -1.3610                                     
REMARK   3      L13:   0.2552 L23:  -1.0976                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1447 S12:   0.3512 S13:  -0.1851                       
REMARK   3      S21:  -0.5236 S22:  -0.1618 S23:   0.0779                       
REMARK   3      S31:   0.2879 S32:   0.0762 S33:  -0.0007                       
REMARK   3   TLS GROUP : 26                                                     
REMARK   3    SELECTION: chain 'B' and (resid 321 through 396 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  18.7069 -41.7417 -10.0950              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3227 T22:   0.3145                                     
REMARK   3      T33:   0.4165 T12:   0.0541                                     
REMARK   3      T13:  -0.0178 T23:   0.0553                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.6276 L22:   1.9953                                     
REMARK   3      L33:   4.5763 L12:   0.5660                                     
REMARK   3      L13:  -0.2765 L23:  -0.7663                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0397 S12:  -0.0554 S13:  -0.2614                       
REMARK   3      S21:   0.2330 S22:   0.0857 S23:   0.0732                       
REMARK   3      S31:   0.1541 S32:  -0.0446 S33:  -0.0545                       
REMARK   3   TLS GROUP : 27                                                     
REMARK   3    SELECTION: chain 'J' and (resid 9 through 87 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  50.0767 -11.6358  30.1254              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5175 T22:   0.4595                                     
REMARK   3      T33:   0.3311 T12:   0.0324                                     
REMARK   3      T13:  -0.0324 T23:   0.0170                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.5332 L22:   3.7373                                     
REMARK   3      L33:   4.3088 L12:   1.4060                                     
REMARK   3      L13:  -1.0346 L23:   0.2084                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1290 S12:  -0.4103 S13:  -0.6074                       
REMARK   3      S21:   0.3412 S22:  -0.1615 S23:  -0.1995                       
REMARK   3      S31:   0.8093 S32:  -0.1836 S33:   0.1075                       
REMARK   3   TLS GROUP : 28                                                     
REMARK   3    SELECTION: chain 'J' and (resid 88 through 238 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  59.7650  18.5891  28.9862              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1757 T22:   0.4780                                     
REMARK   3      T33:   0.3205 T12:  -0.0776                                     
REMARK   3      T13:   0.0234 T23:   0.0072                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.4480 L22:   4.1872                                     
REMARK   3      L33:   1.8195 L12:  -0.9269                                     
REMARK   3      L13:   1.1901 L23:  -1.1085                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0448 S12:  -0.2001 S13:   0.2170                       
REMARK   3      S21:   0.4744 S22:  -0.0562 S23:  -0.1872                       
REMARK   3      S31:  -0.1787 S32:   0.2853 S33:   0.1295                       
REMARK   3   TLS GROUP : 29                                                     
REMARK   3    SELECTION: chain 'J' and (resid 239 through 273 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  49.8764  29.7152  13.1959              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2425 T22:   0.3936                                     
REMARK   3      T33:   0.3441 T12:   0.0252                                     
REMARK   3      T13:   0.0487 T23:  -0.0311                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.9454 L22:   7.5749                                     
REMARK   3      L33:   6.7144 L12:  -1.2805                                     
REMARK   3      L13:   0.7484 L23:   2.4898                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1607 S12:   0.0255 S13:   0.4590                       
REMARK   3      S21:  -0.2700 S22:   0.3595 S23:  -0.3990                       
REMARK   3      S31:  -0.0848 S32:   0.2469 S33:  -0.3486                       
REMARK   3   TLS GROUP : 30                                                     
REMARK   3    SELECTION: chain 'J' and (resid 274 through 358 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  37.0596  35.1458  11.9146              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2456 T22:   0.3637                                     
REMARK   3      T33:   0.4699 T12:  -0.0310                                     
REMARK   3      T13:  -0.0138 T23:   0.0557                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.9785 L22:   2.9659                                     
REMARK   3      L33:   2.5723 L12:  -1.5909                                     
REMARK   3      L13:  -0.1748 L23:   0.3266                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0969 S12:   0.3534 S13:   0.1788                       
REMARK   3      S21:  -0.2117 S22:  -0.1636 S23:  -0.0231                       
REMARK   3      S31:  -0.3317 S32:  -0.0411 S33:   0.0278                       
REMARK   3   TLS GROUP : 31                                                     
REMARK   3    SELECTION: chain 'J' and (resid 359 through 396 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  20.8433  35.5972  18.4158              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3664 T22:   0.4264                                     
REMARK   3      T33:   0.5199 T12:   0.0714                                     
REMARK   3      T13:  -0.0248 T23:   0.0289                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.3480 L22:   2.8266                                     
REMARK   3      L33:   4.3453 L12:   1.9256                                     
REMARK   3      L13:  -1.1135 L23:  -0.8655                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1458 S12:   0.5886 S13:  -0.1651                       
REMARK   3      S21:  -0.1172 S22:  -0.0417 S23:  -0.2072                       
REMARK   3      S31:  -0.1944 S32:  -0.6542 S33:   0.0119                       
REMARK   3   TLS GROUP : 32                                                     
REMARK   3    SELECTION: chain 'A' and (resid 9 through 358 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  61.8047 -45.8587 -41.9860              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3215 T22:   0.4023                                     
REMARK   3      T33:   0.4127 T12:   0.0127                                     
REMARK   3      T13:   0.1185 T23:   0.1255                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0307 L22:   1.6791                                     
REMARK   3      L33:   2.0888 L12:  -0.2883                                     
REMARK   3      L13:  -0.4712 L23:   0.3660                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0152 S12:   0.1729 S13:   0.0636                       
REMARK   3      S21:  -0.3399 S22:  -0.0663 S23:  -0.2571                       
REMARK   3      S31:  -0.0372 S32:   0.1655 S33:   0.0611                       
REMARK   3   TLS GROUP : 33                                                     
REMARK   3    SELECTION: chain 'A' and (resid 359 through 396 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  62.9266 -37.4446  -7.3700              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4612 T22:   0.4527                                     
REMARK   3      T33:   0.3999 T12:  -0.0745                                     
REMARK   3      T13:   0.0615 T23:  -0.0313                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.9375 L22:   3.6188                                     
REMARK   3      L33:   5.3917 L12:  -1.9586                                     
REMARK   3      L13:   1.4811 L23:  -0.5570                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2361 S12:  -0.4732 S13:   0.2272                       
REMARK   3      S21:  -0.2204 S22:  -0.3179 S23:   0.0746                       
REMARK   3      S31:   0.7199 S32:  -0.0474 S33:  -0.0460                       
REMARK   3   TLS GROUP : 34                                                     
REMARK   3    SELECTION: chain 'E' and (resid 55 through 80 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  28.7665 -64.1921 -19.1533              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7141 T22:   0.4732                                     
REMARK   3      T33:   0.4387 T12:   0.0553                                     
REMARK   3      T13:  -0.2632 T23:   0.0408                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2202 L22:   0.6752                                     
REMARK   3      L33:   0.5140 L12:  -0.3440                                     
REMARK   3      L13:   0.2864 L23:  -0.4767                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4107 S12:   0.1439 S13:  -0.3452                       
REMARK   3      S21:  -0.5247 S22:  -0.1815 S23:   0.3641                       
REMARK   3      S31:   0.0772 S32:  -0.7647 S33:  -0.3796                       
REMARK   3   TLS GROUP : 35                                                     
REMARK   3    SELECTION: chain 'E' and (resid 81 through 94 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  31.4759 -71.6615 -10.5461              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4082 T22:   0.5506                                     
REMARK   3      T33:   0.3487 T12:   0.0421                                     
REMARK   3      T13:  -0.0423 T23:   0.1873                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.3532 L22:   8.1749                                     
REMARK   3      L33:   4.4346 L12:  -2.1627                                     
REMARK   3      L13:  -2.3932 L23:   3.5260                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3191 S12:  -0.1628 S13:  -0.5225                       
REMARK   3      S21:  -0.2226 S22:  -0.0026 S23:   0.8293                       
REMARK   3      S31:   0.5025 S32:   0.6746 S33:   0.2307                       
REMARK   3   TLS GROUP : 36                                                     
REMARK   3    SELECTION: chain 'E' and (resid 95 through 118 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  38.3815 -65.2959 -20.1886              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4636 T22:   0.2240                                     
REMARK   3      T33:   0.6279 T12:  -0.0193                                     
REMARK   3      T13:  -0.0032 T23:  -0.0191                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.6660 L22:   0.6154                                     
REMARK   3      L33:   2.4215 L12:  -0.8573                                     
REMARK   3      L13:   1.3570 L23:   0.7585                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2742 S12:  -0.1088 S13:  -0.1948                       
REMARK   3      S21:  -0.0813 S22:  -0.3972 S23:   0.2906                       
REMARK   3      S31:   0.3783 S32:  -0.1298 S33:  -0.0399                       
REMARK   3   TLS GROUP : 37                                                     
REMARK   3    SELECTION: chain 'E' and (resid 119 through 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  39.7465 -60.9677  -9.2746              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5210 T22:   0.2308                                     
REMARK   3      T33:   0.3155 T12:   0.0561                                     
REMARK   3      T13:   0.1271 T23:   0.1275                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.1630 L22:   3.3485                                     
REMARK   3      L33:   3.8777 L12:   0.5102                                     
REMARK   3      L13:   2.2267 L23:   0.2717                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1303 S12:  -0.5204 S13:   0.8928                       
REMARK   3      S21:   0.4289 S22:  -0.5198 S23:   0.2187                       
REMARK   3      S31:  -1.1644 S32:  -0.1537 S33:   0.3338                       
REMARK   3   TLS GROUP : 38                                                     
REMARK   3    SELECTION: chain 'E' and (resid 143 through 157 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  55.6938 -70.4949 -14.6333              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3269 T22:   0.4441                                     
REMARK   3      T33:   0.4691 T12:   0.0662                                     
REMARK   3      T13:   0.0158 T23:   0.1499                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4316 L22:   6.0923                                     
REMARK   3      L33:   6.5712 L12:   1.5626                                     
REMARK   3      L13:  -0.0026 L23:  -1.7720                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2029 S12:  -0.3241 S13:  -0.3791                       
REMARK   3      S21:   0.0158 S22:  -0.3069 S23:  -1.5108                       
REMARK   3      S31:   0.9420 S32:   1.0442 S33:   0.5595                       
REMARK   3   TLS GROUP : 39                                                     
REMARK   3    SELECTION: chain 'E' and (resid 158 through 177 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  55.8715 -65.2027 -24.4207              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3997 T22:   0.3312                                     
REMARK   3      T33:   0.5324 T12:   0.0130                                     
REMARK   3      T13:   0.1222 T23:   0.0925                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5367 L22:   1.4604                                     
REMARK   3      L33:   4.6495 L12:   0.1593                                     
REMARK   3      L13:   0.5030 L23:   2.3389                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1334 S12:  -0.1563 S13:  -0.2500                       
REMARK   3      S21:  -0.2598 S22:   0.5345 S23:   0.1755                       
REMARK   3      S31:  -0.0827 S32:   0.7751 S33:   0.1018                       
REMARK   3   TLS GROUP : 40                                                     
REMARK   3    SELECTION: chain 'E' and (resid 178 through 191 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  45.5349 -68.6450  -8.2613              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4226 T22:   0.2816                                     
REMARK   3      T33:   0.4552 T12:   0.0570                                     
REMARK   3      T13:   0.0431 T23:   0.0019                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.4879 L22:   1.5496                                     
REMARK   3      L33:   0.9133 L12:   2.3313                                     
REMARK   3      L13:  -1.1416 L23:  -0.7471                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0933 S12:  -0.2484 S13:  -0.2425                       
REMARK   3      S21:   0.1851 S22:  -0.0324 S23:  -0.6210                       
REMARK   3      S31:   0.2751 S32:   0.4636 S33:   0.1996                       
REMARK   3   TLS GROUP : 41                                                     
REMARK   3    SELECTION: chain 'E' and (resid 192 through 203 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  41.8498 -57.4182   1.3847              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6824 T22:   0.7233                                     
REMARK   3      T33:   0.3021 T12:  -0.1231                                     
REMARK   3      T13:   0.0610 T23:  -0.1007                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9941 L22:   3.2304                                     
REMARK   3      L33:   3.5981 L12:   1.6752                                     
REMARK   3      L13:  -1.3303 L23:   2.4657                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.7034 S12:  -0.9703 S13:   0.7368                       
REMARK   3      S21:   0.4586 S22:  -0.2785 S23:  -0.1197                       
REMARK   3      S31:  -0.7631 S32:   0.1441 S33:   0.0612                       
REMARK   3   TLS GROUP : 42                                                     
REMARK   3    SELECTION: chain 'H' and (resid 56 through 103 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.7146  -3.4290  39.6719              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7974 T22:   0.3896                                     
REMARK   3      T33:   0.4440 T12:  -0.0275                                     
REMARK   3      T13:   0.0078 T23:   0.0130                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.5070 L22:   2.0561                                     
REMARK   3      L33:   1.8348 L12:  -1.1915                                     
REMARK   3      L13:  -1.4822 L23:  -0.8588                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1196 S12:  -0.9160 S13:  -0.2547                       
REMARK   3      S21:   1.0966 S22:   0.3503 S23:   0.4823                       
REMARK   3      S31:   0.2300 S32:   0.3282 S33:  -0.0228                       
REMARK   3   TLS GROUP : 43                                                     
REMARK   3    SELECTION: chain 'H' and (resid 104 through 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -6.7924   4.4831  37.6457              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7591 T22:   0.3322                                     
REMARK   3      T33:   0.5871 T12:   0.0373                                     
REMARK   3      T13:  -0.1509 T23:  -0.0025                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7383 L22:   4.1656                                     
REMARK   3      L33:   5.5285 L12:   3.0942                                     
REMARK   3      L13:  -0.0732 L23:  -1.1714                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2748 S12:  -0.4087 S13:   0.2092                       
REMARK   3      S21:   1.9785 S22:   0.2433 S23:  -0.1749                       
REMARK   3      S31:  -0.6013 S32:  -0.9052 S33:  -0.4273                       
REMARK   3   TLS GROUP : 44                                                     
REMARK   3    SELECTION: chain 'H' and (resid 143 through 203 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   0.9409   4.2079  27.3346              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3707 T22:   0.4380                                     
REMARK   3      T33:   0.4830 T12:  -0.0800                                     
REMARK   3      T13:  -0.1361 T23:   0.0143                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5647 L22:   1.9876                                     
REMARK   3      L33:   4.7800 L12:  -0.8568                                     
REMARK   3      L13:   1.7467 L23:  -1.3248                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0791 S12:  -0.0406 S13:   0.1508                       
REMARK   3      S21:   0.4012 S22:   0.1331 S23:  -0.3593                       
REMARK   3      S31:  -0.1799 S32:   0.6697 S33:  -0.1672                       
REMARK   3   TLS GROUP : 45                                                     
REMARK   3    SELECTION: chain 'K' and (resid 55 through 64 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  38.3609   4.4357 -11.5074              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4163 T22:   0.5810                                     
REMARK   3      T33:   0.7704 T12:  -0.0311                                     
REMARK   3      T13:  -0.1596 T23:   0.1537                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9229 L22:   8.1959                                     
REMARK   3      L33:   7.3944 L12:  -3.3819                                     
REMARK   3      L13:   0.1081 L23:  -2.9572                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0082 S12:  -0.4006 S13:  -0.5324                       
REMARK   3      S21:  -0.8619 S22:   0.9946 S23:   1.2109                       
REMARK   3      S31:   0.7954 S32:  -0.9073 S33:  -0.8638                       
REMARK   3   TLS GROUP : 46                                                     
REMARK   3    SELECTION: chain 'K' and (resid 65 through 80 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  37.9970  17.6730 -20.7243              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6701 T22:   0.7001                                     
REMARK   3      T33:   0.5712 T12:  -0.0627                                     
REMARK   3      T13:  -0.0223 T23:   0.2364                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.8553 L22:   5.9253                                     
REMARK   3      L33:   1.3791 L12:  -2.0293                                     
REMARK   3      L13:   0.2931 L23:   1.3038                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3339 S12:   0.6325 S13:   0.0470                       
REMARK   3      S21:  -1.0446 S22:   0.5737 S23:   1.2599                       
REMARK   3      S31:   0.0892 S32:  -0.4971 S33:  -0.7576                       
REMARK   3   TLS GROUP : 47                                                     
REMARK   3    SELECTION: chain 'K' and (resid 81 through 103 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  26.6188   8.5995 -15.1902              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5280 T22:   0.3576                                     
REMARK   3      T33:   0.5872 T12:   0.0065                                     
REMARK   3      T13:  -0.1602 T23:  -0.0970                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.4255 L22:   4.4917                                     
REMARK   3      L33:   7.5064 L12:  -1.0002                                     
REMARK   3      L13:   1.6014 L23:  -0.5675                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.7774 S12:   1.2886 S13:  -0.4639                       
REMARK   3      S21:  -1.0860 S22:   0.0408 S23:  -0.2573                       
REMARK   3      S31:   0.7010 S32:   0.3108 S33:  -0.7042                       
REMARK   3   TLS GROUP : 48                                                     
REMARK   3    SELECTION: chain 'K' and (resid 104 through 142 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  29.7457  16.2533 -10.3441              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3443 T22:   0.3121                                     
REMARK   3      T33:   0.5362 T12:  -0.0177                                     
REMARK   3      T13:  -0.0069 T23:   0.0537                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.9435 L22:   1.9668                                     
REMARK   3      L33:   7.7648 L12:  -0.7524                                     
REMARK   3      L13:   1.4903 L23:   0.0352                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0838 S12:   0.6268 S13:   0.7825                       
REMARK   3      S21:  -0.4255 S22:  -0.1530 S23:  -0.3184                       
REMARK   3      S31:  -0.3474 S32:   0.5222 S33:   0.0958                       
REMARK   3   TLS GROUP : 49                                                     
REMARK   3    SELECTION: chain 'K' and (resid 143 through 149 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  20.4271   9.3926  -2.6351              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0863 T22:   0.4243                                     
REMARK   3      T33:   0.3537 T12:   0.0851                                     
REMARK   3      T13:  -0.0654 T23:  -0.1460                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.2386 L22:   1.7965                                     
REMARK   3      L33:   4.3469 L12:  -1.1033                                     
REMARK   3      L13:   1.8251 L23:   0.0586                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.8729 S12:   0.1937 S13:  -0.5569                       
REMARK   3      S21:  -0.2726 S22:  -0.0191 S23:  -0.1477                       
REMARK   3      S31:   0.2597 S32:  -0.1823 S33:   0.3873                       
REMARK   3   TLS GROUP : 50                                                     
REMARK   3    SELECTION: chain 'K' and (resid 150 through 156 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  22.5079   0.7035   9.3062              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5867 T22:   0.5768                                     
REMARK   3      T33:   0.3709 T12:  -0.2509                                     
REMARK   3      T13:   0.0585 T23:   0.0394                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.1020 L22:   6.8395                                     
REMARK   3      L33:   4.1088 L12:   3.4551                                     
REMARK   3      L13:   0.4452 L23:  -1.4204                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2276 S12:  -0.5952 S13:  -0.9438                       
REMARK   3      S21:   0.3038 S22:  -0.4325 S23:  -0.2349                       
REMARK   3      S31:   1.2955 S32:  -1.3955 S33:   0.4366                       
REMARK   3   TLS GROUP : 51                                                     
REMARK   3    SELECTION: chain 'K' and (resid 157 through 203 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  23.6218  12.7819  -1.6871              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3703 T22:   0.3286                                     
REMARK   3      T33:   0.5160 T12:  -0.0079                                     
REMARK   3      T13:  -0.0960 T23:  -0.0659                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7469 L22:   0.2884                                     
REMARK   3      L33:   4.7757 L12:   0.1501                                     
REMARK   3      L13:   1.7848 L23:  -0.3464                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3808 S12:  -0.3220 S13:   0.4551                       
REMARK   3      S21:  -0.0776 S22:   0.1789 S23:  -0.1713                       
REMARK   3      S31:  -0.4511 S32:  -0.6022 S33:   0.2311                       
REMARK   3   TLS GROUP : 52                                                     
REMARK   3    SELECTION: chain 'C' and (resid 55 through 80 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  49.7479 -13.4106  -7.6240              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6373 T22:   0.4092                                     
REMARK   3      T33:   0.8280 T12:   0.2319                                     
REMARK   3      T13:   0.0171 T23:  -0.0540                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0621 L22:   0.6672                                     
REMARK   3      L33:   0.5746 L12:  -0.1588                                     
REMARK   3      L13:   0.1704 L23:  -0.6053                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3743 S12:   0.0264 S13:   0.3047                       
REMARK   3      S21:  -0.3817 S22:  -0.4686 S23:  -0.4032                       
REMARK   3      S31:  -0.2922 S32:   0.3795 S33:  -0.0879                       
REMARK   3   TLS GROUP : 53                                                     
REMARK   3    SELECTION: chain 'C' and (resid 81 through 93 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  48.4574 -11.8297   4.1690              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3701 T22:   0.4777                                     
REMARK   3      T33:   0.3835 T12:   0.0745                                     
REMARK   3      T13:  -0.1191 T23:  -0.1373                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4638 L22:   3.8364                                     
REMARK   3      L33:   2.3066 L12:  -0.2744                                     
REMARK   3      L13:   0.0633 L23:  -2.9044                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0361 S12:   0.2083 S13:   0.3858                       
REMARK   3      S21:  -0.3416 S22:  -0.5075 S23:  -0.6919                       
REMARK   3      S31:   0.1624 S32:   0.4091 S33:   0.0709                       
REMARK   3   TLS GROUP : 54                                                     
REMARK   3    SELECTION: chain 'C' and (resid 94 through 128 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  41.1532 -14.6799  -5.9200              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5209 T22:   0.3096                                     
REMARK   3      T33:   0.3410 T12:   0.0201                                     
REMARK   3      T13:  -0.0799 T23:  -0.0644                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.4338 L22:   0.4870                                     
REMARK   3      L33:   2.5180 L12:   0.4538                                     
REMARK   3      L13:  -0.1871 L23:  -0.1569                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0940 S12:   0.1838 S13:  -0.4909                       
REMARK   3      S21:   0.1713 S22:  -0.2353 S23:  -0.5847                       
REMARK   3      S31:  -0.0576 S32:   0.3359 S33:   0.1075                       
REMARK   3   TLS GROUP : 55                                                     
REMARK   3    SELECTION: chain 'C' and (resid 129 through 156 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  30.1946 -15.9483   3.8884              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3505 T22:   0.2920                                     
REMARK   3      T33:   0.2508 T12:  -0.0577                                     
REMARK   3      T13:  -0.1089 T23:  -0.0013                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.9197 L22:   1.3613                                     
REMARK   3      L33:   3.5686 L12:  -1.3813                                     
REMARK   3      L13:   1.5840 L23:  -0.6425                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1393 S12:  -1.0966 S13:   0.0527                       
REMARK   3      S21:   0.1124 S22:   0.1668 S23:  -0.0567                       
REMARK   3      S31:  -0.0217 S32:   0.0017 S33:  -0.2631                       
REMARK   3   TLS GROUP : 56                                                     
REMARK   3    SELECTION: chain 'C' and (resid 157 through 185 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  24.5517 -11.5674  -5.2585              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4341 T22:   0.4059                                     
REMARK   3      T33:   0.3164 T12:  -0.0241                                     
REMARK   3      T13:   0.0348 T23:   0.0173                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.0477 L22:   3.5679                                     
REMARK   3      L33:   0.4442 L12:  -0.0654                                     
REMARK   3      L13:   1.0990 L23:   0.3789                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2268 S12:  -0.4214 S13:   0.0272                       
REMARK   3      S21:  -0.2630 S22:   0.0433 S23:   0.6393                       
REMARK   3      S31:   0.2632 S32:  -0.4328 S33:  -0.2086                       
REMARK   3   TLS GROUP : 57                                                     
REMARK   3    SELECTION: chain 'C' and (resid 186 through 203 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  39.4114 -25.4332  10.1677              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8777 T22:   0.4610                                     
REMARK   3      T33:   0.3968 T12:   0.0121                                     
REMARK   3      T13:  -0.0956 T23:   0.0856                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.4492 L22:   8.4931                                     
REMARK   3      L33:   5.4180 L12:  -2.7217                                     
REMARK   3      L13:  -1.0548 L23:   1.7329                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.7166 S12:  -0.9182 S13:  -0.8056                       
REMARK   3      S21:   1.3255 S22:   0.6939 S23:   0.6310                       
REMARK   3      S31:   1.0593 S32:  -0.8230 S33:  -0.1809                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4NEE COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 02-DEC-13.                  
REMARK 100 THE RCSB ID CODE IS RCSB083097.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 10-JUL-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 200                                
REMARK 200  PH                             : 9.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 22-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000                              
REMARK 200  MONOCHROMATOR                  : SI (111)                           
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 300 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 80188                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.884                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.7                               
REMARK 200  DATA REDUNDANCY                : 5.200                              
REMARK 200  R MERGE                    (I) : 0.18400                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.88                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.00                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 80.2                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 3REA                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 57.76                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.91                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 70MM CHES PH 9.5, 140MM NACL, 7%         
REMARK 280  PEG8000, 21% GLYCEROL, 0.2MM INOSITOL HEXAKISPHOSPHATE (IP6),       
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 288K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       54.77900            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      100.10200            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       84.01700            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000      100.10200            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       54.77900            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       84.01700            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3, 4                                              
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7140 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 29580 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -21.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D, B, C                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7250 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 29890 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -22.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: L, J, K                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7110 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 29270 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -22.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F, A, E                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 4                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7000 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 29640 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -22.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: I, G, H                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY G    -1                                                      
REMARK 465     ALA G     0                                                      
REMARK 465     MET G     1                                                      
REMARK 465     PRO G     2                                                      
REMARK 465     ALA G     3                                                      
REMARK 465     VAL G     4                                                      
REMARK 465     SER G     5                                                      
REMARK 465     LYS G     6                                                      
REMARK 465     GLY G     7                                                      
REMARK 465     GLU G     8                                                      
REMARK 465     GLY B    -1                                                      
REMARK 465     ALA B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     PRO B     2                                                      
REMARK 465     ALA B     3                                                      
REMARK 465     VAL B     4                                                      
REMARK 465     SER B     5                                                      
REMARK 465     LYS B     6                                                      
REMARK 465     GLY B     7                                                      
REMARK 465     GLU B     8                                                      
REMARK 465     GLY J    -1                                                      
REMARK 465     ALA J     0                                                      
REMARK 465     MET J     1                                                      
REMARK 465     PRO J     2                                                      
REMARK 465     ALA J     3                                                      
REMARK 465     VAL J     4                                                      
REMARK 465     SER J     5                                                      
REMARK 465     LYS J     6                                                      
REMARK 465     GLY J     7                                                      
REMARK 465     GLU J     8                                                      
REMARK 465     GLY A    -1                                                      
REMARK 465     ALA A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     PRO A     2                                                      
REMARK 465     ALA A     3                                                      
REMARK 465     VAL A     4                                                      
REMARK 465     SER A     5                                                      
REMARK 465     LYS A     6                                                      
REMARK 465     GLY A     7                                                      
REMARK 465     GLU A     8                                                      
REMARK 465     ASP A    47                                                      
REMARK 465     LYS A    48                                                      
REMARK 465     ALA A    49                                                      
REMARK 465     GLY E    48                                                      
REMARK 465     UNK E    49                                                      
REMARK 465     UNK E    50                                                      
REMARK 465     UNK E    51                                                      
REMARK 465     UNK E    52                                                      
REMARK 465     UNK E    53                                                      
REMARK 465     UNK E    54                                                      
REMARK 465     GLU E    63                                                      
REMARK 465     GLU E    64                                                      
REMARK 465     GLU E    65                                                      
REMARK 465     VAL E    66                                                      
REMARK 465     GLY E    67                                                      
REMARK 465     PHE E    68                                                      
REMARK 465     PRO E    69                                                      
REMARK 465     VAL E    70                                                      
REMARK 465     THR E    71                                                      
REMARK 465     GLY H    54                                                      
REMARK 465     UNK H    55                                                      
REMARK 465     GLU H    67A                                                     
REMARK 465     GLU H    67B                                                     
REMARK 465     GLU H    67C                                                     
REMARK 465     VAL H    67D                                                     
REMARK 465     GLY H    67E                                                     
REMARK 465     PHE H    67F                                                     
REMARK 465     PRO H    67G                                                     
REMARK 465     VAL H    67H                                                     
REMARK 465     THR H    67I                                                     
REMARK 465     PRO H    67J                                                     
REMARK 465     GLN H    67K                                                     
REMARK 465     GLY K    54                                                      
REMARK 465     GLU K    67A                                                     
REMARK 465     GLU K    67B                                                     
REMARK 465     GLU K    67C                                                     
REMARK 465     VAL K    67D                                                     
REMARK 465     GLY K    67E                                                     
REMARK 465     PHE K    67F                                                     
REMARK 465     PRO K    67G                                                     
REMARK 465     VAL K    67H                                                     
REMARK 465     THR K    67I                                                     
REMARK 465     PRO K    67J                                                     
REMARK 465     GLY C    48                                                      
REMARK 465     UNK C    49                                                      
REMARK 465     UNK C    50                                                      
REMARK 465     UNK C    51                                                      
REMARK 465     UNK C    52                                                      
REMARK 465     UNK C    53                                                      
REMARK 465     UNK C    54                                                      
REMARK 465     GLU C    63                                                      
REMARK 465     GLU C    64                                                      
REMARK 465     GLU C    65                                                      
REMARK 465     VAL C    66                                                      
REMARK 465     GLY C    67                                                      
REMARK 465     PHE C    68                                                      
REMARK 465     PRO C    69                                                      
REMARK 465     VAL C    70                                                      
REMARK 465     THR C    71                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OH   TYR A    95     OD2  ASP A   119              1.95            
REMARK 500   OG   SER A   209     NZ   LYS A   260              2.04            
REMARK 500   OH   TYR J    95     OD1  ASP J   119              2.04            
REMARK 500   OD2  ASP B   382     OG   SER B   384              2.07            
REMARK 500   NZ   LYS F    56     OD1  ASN F    77              2.09            
REMARK 500   O    ILE J   236     OG   SER J   239              2.10            
REMARK 500   O    ARG A   235     OG   SER A   239              2.12            
REMARK 500   O    THR A   208     OG1  THR A   212              2.13            
REMARK 500   O    TYR A    95     OG   SER A    99              2.13            
REMARK 500   O    HIS F    46     NH1  ARG E   178              2.13            
REMARK 500   NZ   LYS L    18     OD2  ASP L   115              2.15            
REMARK 500   OH   TYR B    95     OD2  ASP B   119              2.16            
REMARK 500   NZ   LYS I    56     OD1  ASN I    77              2.16            
REMARK 500   NH2  ARG E   134     OE2  GLU E   179              2.17            
REMARK 500   OG   SER J   209     NZ   LYS J   260              2.18            
REMARK 500   OD2  ASP G   382     OG   SER G   384              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ALA D  63     -126.07     54.67                                   
REMARK 500    HIS D  96       97.83     57.66                                   
REMARK 500    ARG D 124      -73.89   -105.91                                   
REMARK 500    ARG L  53       -9.91     81.59                                   
REMARK 500    ALA L  63     -126.41     54.25                                   
REMARK 500    HIS L  96      102.39     56.62                                   
REMARK 500    ARG L 124      -76.90   -104.15                                   
REMARK 500    ALA F  44     -113.10     63.36                                   
REMARK 500    ARG F  53      -49.17     72.59                                   
REMARK 500    ALA F  63     -126.62     54.00                                   
REMARK 500    HIS F  96      100.60     59.03                                   
REMARK 500    ARG F 124      -75.82   -103.24                                   
REMARK 500    ARG I  53     -116.45     40.66                                   
REMARK 500    ALA I  63     -124.26     54.19                                   
REMARK 500    HIS I  96      104.70     60.79                                   
REMARK 500    ARG I 124      -76.21   -107.13                                   
REMARK 500    ASP G 159       -5.72    -40.86                                   
REMARK 500    LEU G 244      -35.76    -35.83                                   
REMARK 500    ARG G 388       33.83    -82.13                                   
REMARK 500    ALA G 389      -36.55   -137.97                                   
REMARK 500    LYS B  45       30.37    -82.86                                   
REMARK 500    ASP B 159       -8.30     73.21                                   
REMARK 500    SER J 239      126.27    -29.84                                   
REMARK 500    ALA J 240     -160.40    -77.21                                   
REMARK 500    ASP J 321       18.97     58.96                                   
REMARK 500    PRO A 125     -164.58    -67.17                                   
REMARK 500    THR A 126      -50.07     60.28                                   
REMARK 500    UNK E  56      -97.53     47.80                                   
REMARK 500    LEU E  97      -35.54     82.50                                   
REMARK 500    UNK H  66      -80.91   -145.49                                   
REMARK 500    UNK K  65       47.47    -84.52                                   
REMARK 500    LEU C  97      -49.92     88.37                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 ASP F   43     ALA F   44                   31.91                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999 THE N-TERMINAL SEQUENCE OF THE PROTEIN is GAMGSACAWLEAQE. DUE TO     
REMARK 999 THE PRESENCE OF A FLEXIBLE LOOP THE REGISTRATION OF THE MODEL        
REMARK 999 SEQUENCE IS UNKNOWN.                                                 
DBREF  4NEE D    1   142  UNP    P62744   AP2S1_RAT        1    142             
DBREF  4NEE L    1   142  UNP    P62744   AP2S1_RAT        1    142             
DBREF  4NEE F    1   142  UNP    P62744   AP2S1_RAT        1    142             
DBREF  4NEE I    1   142  UNP    P62744   AP2S1_RAT        1    142             
DBREF  4NEE G    1   396  UNP    Q66HM2   Q66HM2_RAT       1    396             
DBREF  4NEE B    1   396  UNP    Q66HM2   Q66HM2_RAT       1    396             
DBREF  4NEE J    1   396  UNP    Q66HM2   Q66HM2_RAT       1    396             
DBREF  4NEE A    1   396  UNP    Q66HM2   Q66HM2_RAT       1    396             
DBREF  4NEE E   63   203  UNP    Q90VU7   Q90VU7_9HIV1    63    203             
DBREF  4NEE H   67A  203  UNP    Q90VU7   Q90VU7_9HIV1    63    203             
DBREF  4NEE K   67A  203  UNP    Q90VU7   Q90VU7_9HIV1    63    203             
DBREF  4NEE C   63   203  UNP    Q90VU7   Q90VU7_9HIV1    63    203             
SEQADV 4NEE GLY G   -1  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE ALA G    0  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE GLY B   -1  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE ALA B    0  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE GLY J   -1  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE ALA J    0  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE GLY A   -1  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE ALA A    0  UNP  Q66HM2              CLONING ARTIFACT               
SEQADV 4NEE GLY E   48  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   49  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   50  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   51  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   52  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   53  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   54  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   55  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   56  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   57  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   58  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   59  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   60  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK E   61  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE GLY H   54  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   55  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   56  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   57  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   58  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   59  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   60  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   61  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   62  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   63  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   64  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   65  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   66  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK H   67  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE GLY K   54  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   55  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   56  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   57  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   58  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   59  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   60  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   61  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   62  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   63  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   64  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   65  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   66  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK K   67  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE GLY C   48  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   49  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   50  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   51  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   52  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   53  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   54  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   55  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   56  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   57  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   58  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   59  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   60  UNP  Q90VU7              EXPRESSION TAG                 
SEQADV 4NEE UNK C   61  UNP  Q90VU7              EXPRESSION TAG                 
SEQRES   1 D  142  MET ILE ARG PHE ILE LEU ILE GLN ASN ARG ALA GLY LYS          
SEQRES   2 D  142  THR ARG LEU ALA LYS TRP TYR MET GLN PHE ASP ASP ASP          
SEQRES   3 D  142  GLU LYS GLN LYS LEU ILE GLU GLU VAL HIS ALA VAL VAL          
SEQRES   4 D  142  THR VAL ARG ASP ALA LYS HIS THR ASN PHE VAL GLU PHE          
SEQRES   5 D  142  ARG ASN PHE LYS ILE ILE TYR ARG ARG TYR ALA GLY LEU          
SEQRES   6 D  142  TYR PHE CYS ILE CYS VAL ASP VAL ASN ASP ASN ASN LEU          
SEQRES   7 D  142  ALA TYR LEU GLU ALA ILE HIS ASN PHE VAL GLU VAL LEU          
SEQRES   8 D  142  ASN GLU TYR PHE HIS ASN VAL CYS GLU LEU ASP LEU VAL          
SEQRES   9 D  142  PHE ASN PHE TYR LYS VAL TYR THR VAL VAL ASP GLU MET          
SEQRES  10 D  142  PHE LEU ALA GLY GLU ILE ARG GLU THR SER GLN THR LYS          
SEQRES  11 D  142  VAL LEU LYS GLN LEU LEU MET LEU GLN SER LEU GLU              
SEQRES   1 L  142  MET ILE ARG PHE ILE LEU ILE GLN ASN ARG ALA GLY LYS          
SEQRES   2 L  142  THR ARG LEU ALA LYS TRP TYR MET GLN PHE ASP ASP ASP          
SEQRES   3 L  142  GLU LYS GLN LYS LEU ILE GLU GLU VAL HIS ALA VAL VAL          
SEQRES   4 L  142  THR VAL ARG ASP ALA LYS HIS THR ASN PHE VAL GLU PHE          
SEQRES   5 L  142  ARG ASN PHE LYS ILE ILE TYR ARG ARG TYR ALA GLY LEU          
SEQRES   6 L  142  TYR PHE CYS ILE CYS VAL ASP VAL ASN ASP ASN ASN LEU          
SEQRES   7 L  142  ALA TYR LEU GLU ALA ILE HIS ASN PHE VAL GLU VAL LEU          
SEQRES   8 L  142  ASN GLU TYR PHE HIS ASN VAL CYS GLU LEU ASP LEU VAL          
SEQRES   9 L  142  PHE ASN PHE TYR LYS VAL TYR THR VAL VAL ASP GLU MET          
SEQRES  10 L  142  PHE LEU ALA GLY GLU ILE ARG GLU THR SER GLN THR LYS          
SEQRES  11 L  142  VAL LEU LYS GLN LEU LEU MET LEU GLN SER LEU GLU              
SEQRES   1 F  142  MET ILE ARG PHE ILE LEU ILE GLN ASN ARG ALA GLY LYS          
SEQRES   2 F  142  THR ARG LEU ALA LYS TRP TYR MET GLN PHE ASP ASP ASP          
SEQRES   3 F  142  GLU LYS GLN LYS LEU ILE GLU GLU VAL HIS ALA VAL VAL          
SEQRES   4 F  142  THR VAL ARG ASP ALA LYS HIS THR ASN PHE VAL GLU PHE          
SEQRES   5 F  142  ARG ASN PHE LYS ILE ILE TYR ARG ARG TYR ALA GLY LEU          
SEQRES   6 F  142  TYR PHE CYS ILE CYS VAL ASP VAL ASN ASP ASN ASN LEU          
SEQRES   7 F  142  ALA TYR LEU GLU ALA ILE HIS ASN PHE VAL GLU VAL LEU          
SEQRES   8 F  142  ASN GLU TYR PHE HIS ASN VAL CYS GLU LEU ASP LEU VAL          
SEQRES   9 F  142  PHE ASN PHE TYR LYS VAL TYR THR VAL VAL ASP GLU MET          
SEQRES  10 F  142  PHE LEU ALA GLY GLU ILE ARG GLU THR SER GLN THR LYS          
SEQRES  11 F  142  VAL LEU LYS GLN LEU LEU MET LEU GLN SER LEU GLU              
SEQRES   1 I  142  MET ILE ARG PHE ILE LEU ILE GLN ASN ARG ALA GLY LYS          
SEQRES   2 I  142  THR ARG LEU ALA LYS TRP TYR MET GLN PHE ASP ASP ASP          
SEQRES   3 I  142  GLU LYS GLN LYS LEU ILE GLU GLU VAL HIS ALA VAL VAL          
SEQRES   4 I  142  THR VAL ARG ASP ALA LYS HIS THR ASN PHE VAL GLU PHE          
SEQRES   5 I  142  ARG ASN PHE LYS ILE ILE TYR ARG ARG TYR ALA GLY LEU          
SEQRES   6 I  142  TYR PHE CYS ILE CYS VAL ASP VAL ASN ASP ASN ASN LEU          
SEQRES   7 I  142  ALA TYR LEU GLU ALA ILE HIS ASN PHE VAL GLU VAL LEU          
SEQRES   8 I  142  ASN GLU TYR PHE HIS ASN VAL CYS GLU LEU ASP LEU VAL          
SEQRES   9 I  142  PHE ASN PHE TYR LYS VAL TYR THR VAL VAL ASP GLU MET          
SEQRES  10 I  142  PHE LEU ALA GLY GLU ILE ARG GLU THR SER GLN THR LYS          
SEQRES  11 I  142  VAL LEU LYS GLN LEU LEU MET LEU GLN SER LEU GLU              
SEQRES   1 G  398  GLY ALA MET PRO ALA VAL SER LYS GLY GLU GLY MET ARG          
SEQRES   2 G  398  GLY LEU ALA VAL PHE ILE SER ASP ILE ARG ASN CYS LYS          
SEQRES   3 G  398  SER LYS GLU ALA GLU ILE LYS ARG ILE ASN LYS GLU LEU          
SEQRES   4 G  398  ALA ASN ILE ARG SER LYS PHE LYS GLY ASP LYS ALA LEU          
SEQRES   5 G  398  ASP GLY TYR SER LYS LYS LYS TYR VAL CYS LYS LEU LEU          
SEQRES   6 G  398  PHE ILE PHE LEU LEU GLY HIS ASP ILE ASP PHE GLY HIS          
SEQRES   7 G  398  MET GLU ALA VAL ASN LEU LEU SER SER ASN ARG TYR THR          
SEQRES   8 G  398  GLU LYS GLN ILE GLY TYR LEU PHE ILE SER VAL LEU VAL          
SEQRES   9 G  398  ASN SER ASN SER GLU LEU ILE ARG LEU ILE ASN ASN ALA          
SEQRES  10 G  398  ILE LYS ASN ASP LEU ALA SER ARG ASN PRO THR PHE MET          
SEQRES  11 G  398  GLY LEU ALA LEU HIS CYS ILE ALA ASN VAL GLY SER ARG          
SEQRES  12 G  398  GLU MET ALA GLU ALA PHE ALA GLY GLU ILE PRO LYS ILE          
SEQRES  13 G  398  LEU VAL ALA GLY ASP THR MET ASP SER VAL LYS GLN SER          
SEQRES  14 G  398  ALA ALA LEU CYS LEU LEU ARG LEU TYR ARG THR SER PRO          
SEQRES  15 G  398  ASP LEU VAL PRO MET GLY ASP TRP THR SER ARG VAL VAL          
SEQRES  16 G  398  HIS LEU LEU ASN ASP GLN HIS LEU GLY VAL VAL THR ALA          
SEQRES  17 G  398  ALA THR SER LEU ILE THR THR LEU ALA GLN LYS ASN PRO          
SEQRES  18 G  398  GLU GLU PHE LYS THR SER VAL SER LEU ALA VAL SER ARG          
SEQRES  19 G  398  LEU SER ARG ILE VAL THR SER ALA SER THR ASP LEU GLN          
SEQRES  20 G  398  ASP TYR THR TYR TYR PHE VAL PRO ALA PRO TRP LEU SER          
SEQRES  21 G  398  VAL LYS LEU LEU ARG LEU LEU GLN CYS TYR PRO PRO PRO          
SEQRES  22 G  398  GLU ASP PRO ALA VAL ARG GLY ARG LEU THR GLU CYS LEU          
SEQRES  23 G  398  GLU THR ILE LEU ASN LYS ALA GLN GLU PRO PRO LYS SER          
SEQRES  24 G  398  LYS LYS VAL GLN HIS SER ASN ALA LYS ASN ALA VAL LEU          
SEQRES  25 G  398  PHE GLU ALA ILE SER LEU ILE ILE HIS HIS ASP SER GLU          
SEQRES  26 G  398  PRO ASN LEU LEU VAL ARG ALA CYS ASN GLN LEU GLY GLN          
SEQRES  27 G  398  PHE LEU GLN HIS ARG GLU THR ASN LEU ARG TYR LEU ALA          
SEQRES  28 G  398  LEU GLU SER MET CYS THR LEU ALA SER SER GLU PHE SER          
SEQRES  29 G  398  HIS GLU ALA VAL LYS THR HIS ILE GLU THR VAL ILE ASN          
SEQRES  30 G  398  ALA LEU LYS THR GLU ARG ASP VAL SER VAL ARG GLN ARG          
SEQRES  31 G  398  ALA VAL ASP LEU LEU TYR ALA MET                              
SEQRES   1 B  398  GLY ALA MET PRO ALA VAL SER LYS GLY GLU GLY MET ARG          
SEQRES   2 B  398  GLY LEU ALA VAL PHE ILE SER ASP ILE ARG ASN CYS LYS          
SEQRES   3 B  398  SER LYS GLU ALA GLU ILE LYS ARG ILE ASN LYS GLU LEU          
SEQRES   4 B  398  ALA ASN ILE ARG SER LYS PHE LYS GLY ASP LYS ALA LEU          
SEQRES   5 B  398  ASP GLY TYR SER LYS LYS LYS TYR VAL CYS LYS LEU LEU          
SEQRES   6 B  398  PHE ILE PHE LEU LEU GLY HIS ASP ILE ASP PHE GLY HIS          
SEQRES   7 B  398  MET GLU ALA VAL ASN LEU LEU SER SER ASN ARG TYR THR          
SEQRES   8 B  398  GLU LYS GLN ILE GLY TYR LEU PHE ILE SER VAL LEU VAL          
SEQRES   9 B  398  ASN SER ASN SER GLU LEU ILE ARG LEU ILE ASN ASN ALA          
SEQRES  10 B  398  ILE LYS ASN ASP LEU ALA SER ARG ASN PRO THR PHE MET          
SEQRES  11 B  398  GLY LEU ALA LEU HIS CYS ILE ALA ASN VAL GLY SER ARG          
SEQRES  12 B  398  GLU MET ALA GLU ALA PHE ALA GLY GLU ILE PRO LYS ILE          
SEQRES  13 B  398  LEU VAL ALA GLY ASP THR MET ASP SER VAL LYS GLN SER          
SEQRES  14 B  398  ALA ALA LEU CYS LEU LEU ARG LEU TYR ARG THR SER PRO          
SEQRES  15 B  398  ASP LEU VAL PRO MET GLY ASP TRP THR SER ARG VAL VAL          
SEQRES  16 B  398  HIS LEU LEU ASN ASP GLN HIS LEU GLY VAL VAL THR ALA          
SEQRES  17 B  398  ALA THR SER LEU ILE THR THR LEU ALA GLN LYS ASN PRO          
SEQRES  18 B  398  GLU GLU PHE LYS THR SER VAL SER LEU ALA VAL SER ARG          
SEQRES  19 B  398  LEU SER ARG ILE VAL THR SER ALA SER THR ASP LEU GLN          
SEQRES  20 B  398  ASP TYR THR TYR TYR PHE VAL PRO ALA PRO TRP LEU SER          
SEQRES  21 B  398  VAL LYS LEU LEU ARG LEU LEU GLN CYS TYR PRO PRO PRO          
SEQRES  22 B  398  GLU ASP PRO ALA VAL ARG GLY ARG LEU THR GLU CYS LEU          
SEQRES  23 B  398  GLU THR ILE LEU ASN LYS ALA GLN GLU PRO PRO LYS SER          
SEQRES  24 B  398  LYS LYS VAL GLN HIS SER ASN ALA LYS ASN ALA VAL LEU          
SEQRES  25 B  398  PHE GLU ALA ILE SER LEU ILE ILE HIS HIS ASP SER GLU          
SEQRES  26 B  398  PRO ASN LEU LEU VAL ARG ALA CYS ASN GLN LEU GLY GLN          
SEQRES  27 B  398  PHE LEU GLN HIS ARG GLU THR ASN LEU ARG TYR LEU ALA          
SEQRES  28 B  398  LEU GLU SER MET CYS THR LEU ALA SER SER GLU PHE SER          
SEQRES  29 B  398  HIS GLU ALA VAL LYS THR HIS ILE GLU THR VAL ILE ASN          
SEQRES  30 B  398  ALA LEU LYS THR GLU ARG ASP VAL SER VAL ARG GLN ARG          
SEQRES  31 B  398  ALA VAL ASP LEU LEU TYR ALA MET                              
SEQRES   1 J  398  GLY ALA MET PRO ALA VAL SER LYS GLY GLU GLY MET ARG          
SEQRES   2 J  398  GLY LEU ALA VAL PHE ILE SER ASP ILE ARG ASN CYS LYS          
SEQRES   3 J  398  SER LYS GLU ALA GLU ILE LYS ARG ILE ASN LYS GLU LEU          
SEQRES   4 J  398  ALA ASN ILE ARG SER LYS PHE LYS GLY ASP LYS ALA LEU          
SEQRES   5 J  398  ASP GLY TYR SER LYS LYS LYS TYR VAL CYS LYS LEU LEU          
SEQRES   6 J  398  PHE ILE PHE LEU LEU GLY HIS ASP ILE ASP PHE GLY HIS          
SEQRES   7 J  398  MET GLU ALA VAL ASN LEU LEU SER SER ASN ARG TYR THR          
SEQRES   8 J  398  GLU LYS GLN ILE GLY TYR LEU PHE ILE SER VAL LEU VAL          
SEQRES   9 J  398  ASN SER ASN SER GLU LEU ILE ARG LEU ILE ASN ASN ALA          
SEQRES  10 J  398  ILE LYS ASN ASP LEU ALA SER ARG ASN PRO THR PHE MET          
SEQRES  11 J  398  GLY LEU ALA LEU HIS CYS ILE ALA ASN VAL GLY SER ARG          
SEQRES  12 J  398  GLU MET ALA GLU ALA PHE ALA GLY GLU ILE PRO LYS ILE          
SEQRES  13 J  398  LEU VAL ALA GLY ASP THR MET ASP SER VAL LYS GLN SER          
SEQRES  14 J  398  ALA ALA LEU CYS LEU LEU ARG LEU TYR ARG THR SER PRO          
SEQRES  15 J  398  ASP LEU VAL PRO MET GLY ASP TRP THR SER ARG VAL VAL          
SEQRES  16 J  398  HIS LEU LEU ASN ASP GLN HIS LEU GLY VAL VAL THR ALA          
SEQRES  17 J  398  ALA THR SER LEU ILE THR THR LEU ALA GLN LYS ASN PRO          
SEQRES  18 J  398  GLU GLU PHE LYS THR SER VAL SER LEU ALA VAL SER ARG          
SEQRES  19 J  398  LEU SER ARG ILE VAL THR SER ALA SER THR ASP LEU GLN          
SEQRES  20 J  398  ASP TYR THR TYR TYR PHE VAL PRO ALA PRO TRP LEU SER          
SEQRES  21 J  398  VAL LYS LEU LEU ARG LEU LEU GLN CYS TYR PRO PRO PRO          
SEQRES  22 J  398  GLU ASP PRO ALA VAL ARG GLY ARG LEU THR GLU CYS LEU          
SEQRES  23 J  398  GLU THR ILE LEU ASN LYS ALA GLN GLU PRO PRO LYS SER          
SEQRES  24 J  398  LYS LYS VAL GLN HIS SER ASN ALA LYS ASN ALA VAL LEU          
SEQRES  25 J  398  PHE GLU ALA ILE SER LEU ILE ILE HIS HIS ASP SER GLU          
SEQRES  26 J  398  PRO ASN LEU LEU VAL ARG ALA CYS ASN GLN LEU GLY GLN          
SEQRES  27 J  398  PHE LEU GLN HIS ARG GLU THR ASN LEU ARG TYR LEU ALA          
SEQRES  28 J  398  LEU GLU SER MET CYS THR LEU ALA SER SER GLU PHE SER          
SEQRES  29 J  398  HIS GLU ALA VAL LYS THR HIS ILE GLU THR VAL ILE ASN          
SEQRES  30 J  398  ALA LEU LYS THR GLU ARG ASP VAL SER VAL ARG GLN ARG          
SEQRES  31 J  398  ALA VAL ASP LEU LEU TYR ALA MET                              
SEQRES   1 A  398  GLY ALA MET PRO ALA VAL SER LYS GLY GLU GLY MET ARG          
SEQRES   2 A  398  GLY LEU ALA VAL PHE ILE SER ASP ILE ARG ASN CYS LYS          
SEQRES   3 A  398  SER LYS GLU ALA GLU ILE LYS ARG ILE ASN LYS GLU LEU          
SEQRES   4 A  398  ALA ASN ILE ARG SER LYS PHE LYS GLY ASP LYS ALA LEU          
SEQRES   5 A  398  ASP GLY TYR SER LYS LYS LYS TYR VAL CYS LYS LEU LEU          
SEQRES   6 A  398  PHE ILE PHE LEU LEU GLY HIS ASP ILE ASP PHE GLY HIS          
SEQRES   7 A  398  MET GLU ALA VAL ASN LEU LEU SER SER ASN ARG TYR THR          
SEQRES   8 A  398  GLU LYS GLN ILE GLY TYR LEU PHE ILE SER VAL LEU VAL          
SEQRES   9 A  398  ASN SER ASN SER GLU LEU ILE ARG LEU ILE ASN ASN ALA          
SEQRES  10 A  398  ILE LYS ASN ASP LEU ALA SER ARG ASN PRO THR PHE MET          
SEQRES  11 A  398  GLY LEU ALA LEU HIS CYS ILE ALA ASN VAL GLY SER ARG          
SEQRES  12 A  398  GLU MET ALA GLU ALA PHE ALA GLY GLU ILE PRO LYS ILE          
SEQRES  13 A  398  LEU VAL ALA GLY ASP THR MET ASP SER VAL LYS GLN SER          
SEQRES  14 A  398  ALA ALA LEU CYS LEU LEU ARG LEU TYR ARG THR SER PRO          
SEQRES  15 A  398  ASP LEU VAL PRO MET GLY ASP TRP THR SER ARG VAL VAL          
SEQRES  16 A  398  HIS LEU LEU ASN ASP GLN HIS LEU GLY VAL VAL THR ALA          
SEQRES  17 A  398  ALA THR SER LEU ILE THR THR LEU ALA GLN LYS ASN PRO          
SEQRES  18 A  398  GLU GLU PHE LYS THR SER VAL SER LEU ALA VAL SER ARG          
SEQRES  19 A  398  LEU SER ARG ILE VAL THR SER ALA SER THR ASP LEU GLN          
SEQRES  20 A  398  ASP TYR THR TYR TYR PHE VAL PRO ALA PRO TRP LEU SER          
SEQRES  21 A  398  VAL LYS LEU LEU ARG LEU LEU GLN CYS TYR PRO PRO PRO          
SEQRES  22 A  398  GLU ASP PRO ALA VAL ARG GLY ARG LEU THR GLU CYS LEU          
SEQRES  23 A  398  GLU THR ILE LEU ASN LYS ALA GLN GLU PRO PRO LYS SER          
SEQRES  24 A  398  LYS LYS VAL GLN HIS SER ASN ALA LYS ASN ALA VAL LEU          
SEQRES  25 A  398  PHE GLU ALA ILE SER LEU ILE ILE HIS HIS ASP SER GLU          
SEQRES  26 A  398  PRO ASN LEU LEU VAL ARG ALA CYS ASN GLN LEU GLY GLN          
SEQRES  27 A  398  PHE LEU GLN HIS ARG GLU THR ASN LEU ARG TYR LEU ALA          
SEQRES  28 A  398  LEU GLU SER MET CYS THR LEU ALA SER SER GLU PHE SER          
SEQRES  29 A  398  HIS GLU ALA VAL LYS THR HIS ILE GLU THR VAL ILE ASN          
SEQRES  30 A  398  ALA LEU LYS THR GLU ARG ASP VAL SER VAL ARG GLN ARG          
SEQRES  31 A  398  ALA VAL ASP LEU LEU TYR ALA MET                              
SEQRES   1 E  155  GLY UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 E  155  UNK GLU GLU GLU VAL GLY PHE PRO VAL THR PRO GLN VAL          
SEQRES   3 E  155  PRO LEU ARG PRO MET THR TYR LYS ALA ALA VAL ASP LEU          
SEQRES   4 E  155  SER HIS PHE LEU LYS GLU LYS GLY GLY LEU GLU GLY LEU          
SEQRES   5 E  155  ILE HIS SER GLN ARG ARG GLN ASP ILE LEU ASP LEU TRP          
SEQRES   6 E  155  ILE TYR HIS THR GLN GLY TYR PHE PRO ASP TRP GLN ASN          
SEQRES   7 E  155  TYR THR PRO GLY PRO GLY VAL ARG TYR PRO LEU THR PHE          
SEQRES   8 E  155  GLY TRP CYS TYR LYS LEU VAL PRO VAL GLU PRO ASP LYS          
SEQRES   9 E  155  VAL GLU GLU ALA ASN LYS GLY GLU ASN THR SER LEU LEU          
SEQRES  10 E  155  HIS PRO VAL SER LEU HIS GLY MET ASP ASP PRO GLU ARG          
SEQRES  11 E  155  GLU VAL LEU GLU TRP ARG PHE ASP SER ARG LEU ALA PHE          
SEQRES  12 E  155  HIS HIS VAL ALA ARG GLU LEU HIS PRO GLU TYR PHE              
SEQRES   1 H  155  GLY UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 H  155  UNK GLU GLU GLU VAL GLY PHE PRO VAL THR PRO GLN VAL          
SEQRES   3 H  155  PRO LEU ARG PRO MET THR TYR LYS ALA ALA VAL ASP LEU          
SEQRES   4 H  155  SER HIS PHE LEU LYS GLU LYS GLY GLY LEU GLU GLY LEU          
SEQRES   5 H  155  ILE HIS SER GLN ARG ARG GLN ASP ILE LEU ASP LEU TRP          
SEQRES   6 H  155  ILE TYR HIS THR GLN GLY TYR PHE PRO ASP TRP GLN ASN          
SEQRES   7 H  155  TYR THR PRO GLY PRO GLY VAL ARG TYR PRO LEU THR PHE          
SEQRES   8 H  155  GLY TRP CYS TYR LYS LEU VAL PRO VAL GLU PRO ASP LYS          
SEQRES   9 H  155  VAL GLU GLU ALA ASN LYS GLY GLU ASN THR SER LEU LEU          
SEQRES  10 H  155  HIS PRO VAL SER LEU HIS GLY MET ASP ASP PRO GLU ARG          
SEQRES  11 H  155  GLU VAL LEU GLU TRP ARG PHE ASP SER ARG LEU ALA PHE          
SEQRES  12 H  155  HIS HIS VAL ALA ARG GLU LEU HIS PRO GLU TYR PHE              
SEQRES   1 K  155  GLY UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 K  155  UNK GLU GLU GLU VAL GLY PHE PRO VAL THR PRO GLN VAL          
SEQRES   3 K  155  PRO LEU ARG PRO MET THR TYR LYS ALA ALA VAL ASP LEU          
SEQRES   4 K  155  SER HIS PHE LEU LYS GLU LYS GLY GLY LEU GLU GLY LEU          
SEQRES   5 K  155  ILE HIS SER GLN ARG ARG GLN ASP ILE LEU ASP LEU TRP          
SEQRES   6 K  155  ILE TYR HIS THR GLN GLY TYR PHE PRO ASP TRP GLN ASN          
SEQRES   7 K  155  TYR THR PRO GLY PRO GLY VAL ARG TYR PRO LEU THR PHE          
SEQRES   8 K  155  GLY TRP CYS TYR LYS LEU VAL PRO VAL GLU PRO ASP LYS          
SEQRES   9 K  155  VAL GLU GLU ALA ASN LYS GLY GLU ASN THR SER LEU LEU          
SEQRES  10 K  155  HIS PRO VAL SER LEU HIS GLY MET ASP ASP PRO GLU ARG          
SEQRES  11 K  155  GLU VAL LEU GLU TRP ARG PHE ASP SER ARG LEU ALA PHE          
SEQRES  12 K  155  HIS HIS VAL ALA ARG GLU LEU HIS PRO GLU TYR PHE              
SEQRES   1 C  155  GLY UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 C  155  UNK GLU GLU GLU VAL GLY PHE PRO VAL THR PRO GLN VAL          
SEQRES   3 C  155  PRO LEU ARG PRO MET THR TYR LYS ALA ALA VAL ASP LEU          
SEQRES   4 C  155  SER HIS PHE LEU LYS GLU LYS GLY GLY LEU GLU GLY LEU          
SEQRES   5 C  155  ILE HIS SER GLN ARG ARG GLN ASP ILE LEU ASP LEU TRP          
SEQRES   6 C  155  ILE TYR HIS THR GLN GLY TYR PHE PRO ASP TRP GLN ASN          
SEQRES   7 C  155  TYR THR PRO GLY PRO GLY VAL ARG TYR PRO LEU THR PHE          
SEQRES   8 C  155  GLY TRP CYS TYR LYS LEU VAL PRO VAL GLU PRO ASP LYS          
SEQRES   9 C  155  VAL GLU GLU ALA ASN LYS GLY GLU ASN THR SER LEU LEU          
SEQRES  10 C  155  HIS PRO VAL SER LEU HIS GLY MET ASP ASP PRO GLU ARG          
SEQRES  11 C  155  GLU VAL LEU GLU TRP ARG PHE ASP SER ARG LEU ALA PHE          
SEQRES  12 C  155  HIS HIS VAL ALA ARG GLU LEU HIS PRO GLU TYR PHE              
HELIX    1   1 ASP D   24  ARG D   42  1                                  19    
HELIX    2   2 ASN D   76  HIS D   96  1                                  21    
HELIX    3   3 CYS D   99  ASN D  106  1                                   8    
HELIX    4   4 ASN D  106  MET D  117  1                                  12    
HELIX    5   5 SER D  127  LEU D  141  1                                  15    
HELIX    6   6 ASP L   24  ARG L   42  1                                  19    
HELIX    7   7 ASN L   76  HIS L   96  1                                  21    
HELIX    8   8 CYS L   99  ASN L  106  1                                   8    
HELIX    9   9 ASN L  106  MET L  117  1                                  12    
HELIX   10  10 SER L  127  LEU L  141  1                                  15    
HELIX   11  11 ASP F   24  ARG F   42  1                                  19    
HELIX   12  12 ASN F   76  HIS F   96  1                                  21    
HELIX   13  13 CYS F   99  ASN F  106  1                                   8    
HELIX   14  14 ASN F  106  MET F  117  1                                  12    
HELIX   15  15 SER F  127  LEU F  141  1                                  15    
HELIX   16  16 ASP I   24  ARG I   42  1                                  19    
HELIX   17  17 ASN I   76  HIS I   96  1                                  21    
HELIX   18  18 CYS I   99  ASN I  106  1                                   8    
HELIX   19  19 ASN I  106  MET I  117  1                                  12    
HELIX   20  20 SER I  127  LEU I  141  1                                  15    
HELIX   21  21 MET G   10  CYS G   23  1                                  14    
HELIX   22  22 SER G   25  GLY G   46  1                                  22    
HELIX   23  23 ASP G   51  LEU G   68  1                                  18    
HELIX   24  24 GLY G   75  SER G   84  1                                  10    
HELIX   25  25 ARG G   87  LEU G  101  1                                  15    
HELIX   26  26 ASN G  105  SER G  122  1                                  18    
HELIX   27  27 ASN G  124  GLY G  139  1                                  16    
HELIX   28  28 SER G  140  ALA G  148  1                                   9    
HELIX   29  29 GLY G  149  ALA G  157  1                                   9    
HELIX   30  30 MET G  161  SER G  179  1                                  19    
HELIX   31  31 ASP G  187  HIS G  194  1                                   8    
HELIX   32  32 LEU G  195  ASP G  198  5                                   4    
HELIX   33  33 HIS G  200  ASN G  218  1                                  19    
HELIX   34  34 PRO G  219  PHE G  222  5                                   4    
HELIX   35  35 LYS G  223  THR G  238  1                                  16    
HELIX   36  36 THR G  242  ASP G  246  5                                   5    
HELIX   37  37 ALA G  254  GLN G  266  1                                  13    
HELIX   38  38 ASP G  273  GLU G  293  1                                  21    
HELIX   39  39 LYS G  299  ASP G  321  1                                  23    
HELIX   40  40 GLU G  323  GLN G  339  1                                  17    
HELIX   41  41 GLU G  342  SER G  359  1                                  18    
HELIX   42  42 SER G  362  LYS G  367  1                                   6    
HELIX   43  43 HIS G  369  GLU G  380  1                                  12    
HELIX   44  44 ASP G  382  MET G  396  1                                  15    
HELIX   45  45 MET B   10  CYS B   23  1                                  14    
HELIX   46  46 SER B   25  LYS B   45  1                                  21    
HELIX   47  47 ASP B   51  LEU B   68  1                                  18    
HELIX   48  48 GLY B   75  SER B   85  1                                  11    
HELIX   49  49 ARG B   87  LEU B  101  1                                  15    
HELIX   50  50 ASN B  105  SER B  122  1                                  18    
HELIX   51  51 ASN B  124  GLY B  139  1                                  16    
HELIX   52  52 SER B  140  ALA B  148  1                                   9    
HELIX   53  53 GLY B  149  ALA B  157  1                                   9    
HELIX   54  54 MET B  161  SER B  179  1                                  19    
HELIX   55  55 PRO B  180  VAL B  183  5                                   4    
HELIX   56  56 ASP B  187  LEU B  195  1                                   9    
HELIX   57  57 LEU B  196  ASP B  198  5                                   3    
HELIX   58  58 HIS B  200  ASN B  218  1                                  19    
HELIX   59  59 PRO B  219  PHE B  222  5                                   4    
HELIX   60  60 LYS B  223  THR B  238  1                                  16    
HELIX   61  61 LEU B  244  ASP B  246  5                                   3    
HELIX   62  62 ALA B  254  GLN B  266  1                                  13    
HELIX   63  63 ASP B  273  GLU B  293  1                                  21    
HELIX   64  64 LYS B  299  ASP B  321  1                                  23    
HELIX   65  65 GLU B  323  GLN B  339  1                                  17    
HELIX   66  66 GLU B  342  ALA B  357  1                                  16    
HELIX   67  67 SER B  362  LYS B  367  1                                   6    
HELIX   68  68 HIS B  369  GLU B  380  1                                  12    
HELIX   69  69 ASP B  382  MET B  396  1                                  15    
HELIX   70  70 MET J   10  ASN J   22  1                                  13    
HELIX   71  71 SER J   25  GLY J   46  1                                  22    
HELIX   72  72 ASP J   51  GLY J   69  1                                  19    
HELIX   73  73 GLY J   75  SER J   84  1                                  10    
HELIX   74  74 ARG J   87  LEU J  101  1                                  15    
HELIX   75  75 ASN J  105  SER J  122  1                                  18    
HELIX   76  76 ASN J  124  GLY J  139  1                                  16    
HELIX   77  77 SER J  140  ALA J  148  1                                   9    
HELIX   78  78 GLY J  149  ALA J  157  1                                   9    
HELIX   79  79 MET J  161  SER J  179  1                                  19    
HELIX   80  80 PRO J  180  VAL J  183  5                                   4    
HELIX   81  81 ASP J  187  HIS J  194  1                                   8    
HELIX   82  82 LEU J  195  ASP J  198  5                                   4    
HELIX   83  83 HIS J  200  ASN J  218  1                                  19    
HELIX   84  84 PRO J  219  PHE J  222  5                                   4    
HELIX   85  85 LYS J  223  SER J  239  1                                  17    
HELIX   86  86 LEU J  244  ASP J  246  5                                   3    
HELIX   87  87 ALA J  254  GLN J  266  1                                  13    
HELIX   88  88 ASP J  273  GLU J  293  1                                  21    
HELIX   89  89 LYS J  299  ASP J  321  1                                  23    
HELIX   90  90 GLU J  323  GLN J  339  1                                  17    
HELIX   91  91 GLU J  342  ALA J  357  1                                  16    
HELIX   92  92 SER J  362  LYS J  367  1                                   6    
HELIX   93  93 HIS J  369  GLU J  380  1                                  12    
HELIX   94  94 ASP J  382  MET J  396  1                                  15    
HELIX   95  95 MET A   10  ASN A   22  1                                  13    
HELIX   96  96 SER A   25  LYS A   45  1                                  21    
HELIX   97  97 ASP A   51  LEU A   68  1                                  18    
HELIX   98  98 GLY A   75  SER A   85  1                                  11    
HELIX   99  99 ARG A   87  LEU A  101  1                                  15    
HELIX  100 100 ASN A  105  SER A  122  1                                  18    
HELIX  101 101 THR A  126  GLY A  139  1                                  14    
HELIX  102 102 SER A  140  ALA A  148  1                                   9    
HELIX  103 103 GLY A  149  ALA A  157  1                                   9    
HELIX  104 104 MET A  161  SER A  179  1                                  19    
HELIX  105 105 ASP A  187  LEU A  196  1                                  10    
HELIX  106 106 HIS A  200  ASN A  218  1                                  19    
HELIX  107 107 PRO A  219  PHE A  222  5                                   4    
HELIX  108 108 LYS A  223  SER A  239  1                                  17    
HELIX  109 109 ALA A  254  GLN A  266  1                                  13    
HELIX  110 110 ASP A  273  GLU A  293  1                                  21    
HELIX  111 111 LYS A  299  ASP A  321  1                                  23    
HELIX  112 112 GLU A  323  GLN A  339  1                                  17    
HELIX  113 113 GLU A  342  ALA A  357  1                                  16    
HELIX  114 114 SER A  362  THR A  368  1                                   7    
HELIX  115 115 HIS A  369  GLU A  380  1                                  12    
HELIX  116 116 ASP A  382  MET A  396  1                                  15    
HELIX  117 117 UNK E   56  UNK E   61  1                                   6    
HELIX  118 118 THR E   80  GLY E   95  1                                  16    
HELIX  119 119 SER E  103  GLY E  119  1                                  17    
HELIX  120 120 GLU E  149  LYS E  158  1                                  10    
HELIX  121 121 HIS E  166  HIS E  171  5                                   6    
HELIX  122 122 SER E  187  HIS E  192  1                                   6    
HELIX  123 123 HIS E  193  HIS E  199  1                                   7    
HELIX  124 124 PRO E  200  PHE E  203  5                                   4    
HELIX  125 125 UNK H   57  UNK H   65  1                                   9    
HELIX  126 126 THR H   80  LYS H   94  1                                  15    
HELIX  127 127 SER H  103  GLY H  119  1                                  17    
HELIX  128 128 GLU H  149  ASN H  157  1                                   9    
HELIX  129 129 HIS H  166  HIS H  171  5                                   6    
HELIX  130 130 SER H  187  HIS H  192  1                                   6    
HELIX  131 131 HIS H  193  HIS H  199  1                                   7    
HELIX  132 132 PRO H  200  PHE H  203  5                                   4    
HELIX  133 133 UNK K   56  UNK K   65  1                                  10    
HELIX  134 134 THR K   80  LYS K   94  1                                  15    
HELIX  135 135 SER K  103  GLY K  119  1                                  17    
HELIX  136 136 GLU K  149  ASN K  157  1                                   9    
HELIX  137 137 HIS K  166  HIS K  171  5                                   6    
HELIX  138 138 SER K  187  PHE K  191  5                                   5    
HELIX  139 139 HIS K  193  HIS K  199  1                                   7    
HELIX  140 140 PRO K  200  PHE K  203  5                                   4    
HELIX  141 141 UNK C   56  UNK C   61  1                                   6    
HELIX  142 142 THR C   80  LYS C   94  1                                  15    
HELIX  143 143 SER C  103  GLY C  119  1                                  17    
HELIX  144 144 GLU C  149  ASN C  157  1                                   9    
HELIX  145 145 SER C  187  HIS C  192  1                                   6    
HELIX  146 146 HIS C  193  HIS C  199  1                                   7    
HELIX  147 147 PRO C  200  PHE C  203  5                                   4    
SHEET    1   A 5 THR D  14  TRP D  19  0                                        
SHEET    2   A 5 ILE D   2  ASN D   9 -1  N  ILE D   7   O  LEU D  16           
SHEET    3   A 5 LEU D  65  ASP D  72 -1  O  TYR D  66   N  GLN D   8           
SHEET    4   A 5 PHE D  55  TYR D  62 -1  N  LYS D  56   O  VAL D  71           
SHEET    5   A 5 PHE D  49  GLU D  51 -1  N  VAL D  50   O  ILE D  57           
SHEET    1   B 2 PHE D 118  LEU D 119  0                                        
SHEET    2   B 2 GLU D 122  ILE D 123 -1  O  GLU D 122   N  LEU D 119           
SHEET    1   C 5 THR L  14  TRP L  19  0                                        
SHEET    2   C 5 ILE L   2  ASN L   9 -1  N  ILE L   7   O  LEU L  16           
SHEET    3   C 5 LEU L  65  VAL L  71 -1  O  CYS L  68   N  LEU L   6           
SHEET    4   C 5 LYS L  56  TYR L  62 -1  N  ILE L  58   O  ILE L  69           
SHEET    5   C 5 PHE L  49  GLU L  51 -1  N  VAL L  50   O  ILE L  57           
SHEET    1   D 2 PHE L 118  LEU L 119  0                                        
SHEET    2   D 2 GLU L 122  ILE L 123 -1  O  GLU L 122   N  LEU L 119           
SHEET    1   E 5 THR F  14  TRP F  19  0                                        
SHEET    2   E 5 ILE F   2  ASN F   9 -1  N  ILE F   7   O  ARG F  15           
SHEET    3   E 5 LEU F  65  ASP F  72 -1  O  TYR F  66   N  GLN F   8           
SHEET    4   E 5 PHE F  55  TYR F  62 -1  N  ILE F  58   O  ILE F  69           
SHEET    5   E 5 PHE F  49  GLU F  51 -1  N  VAL F  50   O  ILE F  57           
SHEET    1   F 2 PHE F 118  LEU F 119  0                                        
SHEET    2   F 2 GLU F 122  ILE F 123 -1  O  GLU F 122   N  LEU F 119           
SHEET    1   G 5 THR I  14  TRP I  19  0                                        
SHEET    2   G 5 ILE I   2  ASN I   9 -1  N  ILE I   7   O  LEU I  16           
SHEET    3   G 5 LEU I  65  ASP I  72 -1  O  TYR I  66   N  GLN I   8           
SHEET    4   G 5 PHE I  55  TYR I  62 -1  N  ARG I  60   O  PHE I  67           
SHEET    5   G 5 PHE I  49  PHE I  52 -1  N  VAL I  50   O  ILE I  57           
SHEET    1   H 2 PHE I 118  LEU I 119  0                                        
SHEET    2   H 2 GLU I 122  ILE I 123 -1  O  GLU I 122   N  LEU I 119           
SHEET    1   I 2 THR G 248  TYR G 249  0                                        
SHEET    2   I 2 VAL G 252  PRO G 253 -1  O  VAL G 252   N  TYR G 249           
SHEET    1   J 2 THR B 248  TYR B 249  0                                        
SHEET    2   J 2 VAL B 252  PRO B 253 -1  O  VAL B 252   N  TYR B 249           
SHEET    1   K 2 THR J 248  TYR J 249  0                                        
SHEET    2   K 2 VAL J 252  PRO J 253 -1  O  VAL J 252   N  TYR J 249           
SHEET    1   L 2 THR A 248  TYR A 249  0                                        
SHEET    2   L 2 VAL A 252  PRO A 253 -1  O  VAL A 252   N  TYR A 249           
SHEET    1   M 2 TYR E 143  PRO E 147  0                                        
SHEET    2   M 2 LEU E 181  PHE E 185 -1  O  ARG E 184   N  LYS E 144           
SHEET    1   N 2 TYR H 143  PRO H 147  0                                        
SHEET    2   N 2 LEU H 181  PHE H 185 -1  O  GLU H 182   N  VAL H 146           
SHEET    1   O 2 TYR K 143  PRO K 147  0                                        
SHEET    2   O 2 LEU K 181  PHE K 185 -1  O  GLU K 182   N  VAL K 146           
SHEET    1   P 2 TYR C 143  PRO C 147  0                                        
SHEET    2   P 2 LEU C 181  PHE C 185 -1  O  GLU C 182   N  VAL C 146           
CISPEP   1 GLY E  130    PRO E  131          0         0.46                     
CISPEP   2 UNK H   65    UNK H   66          0         6.67                     
CISPEP   3 GLY H  130    PRO H  131          0        -0.10                     
CISPEP   4 GLY K   95    GLY K   96          0         2.11                     
CISPEP   5 GLY K  130    PRO K  131          0         0.83                     
CISPEP   6 GLY C  130    PRO C  131          0        -0.92                     
CRYST1  109.558  168.034  200.204  90.00  90.00  90.00 P 21 21 21   16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009128  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.005951  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.004995        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system