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Database: PDB
Entry: 4QVF
LinkDB: 4QVF
Original site: 4QVF 
HEADER    APOPTOSIS                               15-JUL-14   4QVF              
TITLE     CRYSTAL STRUCTURE OF BCL-XL IN COMPLEX WITH BIM BH3 DOMAIN            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: BCL-2-LIKE PROTEIN 1;                                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 1-209;                                        
COMPND   5 SYNONYM: BCL2-L-1, APOPTOSIS REGULATOR BCL-X;                        
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: PEPTIDE FROM BCL-2-LIKE PROTEIN 11;                        
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: UNP RESIDUES 141-166;                                      
COMPND  11 SYNONYM: BCL2-L-11, BCL2-INTERACTING MEDIATOR OF CELL DEATH;         
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: BCL2L1, BCL2L, BCLX;                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PETSUMO;                                  
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 SYNTHETIC: YES;                                                      
SOURCE  13 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  14 ORGANISM_COMMON: HUMAN;                                              
SOURCE  15 ORGANISM_TAXID: 9606;                                                
SOURCE  16 OTHER_DETAILS: THIS BIM BH3 SEQUENCE OCCURS NATURALLY IN HUMANS      
KEYWDS    PROTEIN-PEPTIDE COMPLEX, BCL-2 LIKE, HETERODIMER, APOPTOSIS, ANTI-    
KEYWDS   2 APOPTOTIC, BH3 BINDING, BIM BH3                                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    R.SREEKANTH,H.S.YOON                                                  
REVDAT   1   10-JUN-15 4QVF    0                                                
JRNL        AUTH   S.RAJAN,M.CHOI,K.BAEK,H.S.YOON                               
JRNL        TITL   BH3 INDUCED CONFORMATIONAL CHANGES IN BCL-XL REVEALED BY     
JRNL        TITL 2 CRYSTAL STRUCTURE AND COMPARATIVE ANALYSIS                   
JRNL        REF    PROTEINS                                   2015              
JRNL        REFN                   ESSN 1097-0134                               
JRNL        PMID   25907960                                                     
JRNL        DOI    10.1002/PROT.24816                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.53 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8.4_1496)                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.53                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 31.85                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 89.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 22740                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.167                           
REMARK   3   R VALUE            (WORKING SET) : 0.164                           
REMARK   3   FREE R VALUE                     : 0.230                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.040                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 918                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 31.8543 -  2.9282    0.97     3427   158  0.1693 0.2223        
REMARK   3     2  2.9282 -  2.3244    0.98     3468   105  0.1655 0.2282        
REMARK   3     3  2.3244 -  2.0307    0.97     3388   138  0.1574 0.2260        
REMARK   3     4  2.0307 -  1.8450    0.96     3360   151  0.1683 0.2500        
REMARK   3     5  1.8450 -  1.7128    0.97     3339   161  0.1622 0.2516        
REMARK   3     6  1.7128 -  1.6118    0.91     3167   133  0.1534 0.2325        
REMARK   3     7  1.6118 -  1.5311    0.48     1673    72  0.1440 0.2015        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.150            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 23.700           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 13.12                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006           1364                                  
REMARK   3   ANGLE     :  0.878           1847                                  
REMARK   3   CHIRALITY :  0.037            191                                  
REMARK   3   PLANARITY :  0.004            238                                  
REMARK   3   DIHEDRAL  : 13.238            481                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4QVF COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 06-AUG-14.                  
REMARK 100 THE RCSB ID CODE IS RCSB086562.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 26-JUL-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 4.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : NSRRC                              
REMARK 200  BEAMLINE                       : BL13B1                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0000                             
REMARK 200  MONOCHROMATOR                  : GRAPHITE                           
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MOSFLM                             
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 24769                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.530                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 31.850                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.5                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : 0.07000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 11.9000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.53                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.61                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 96.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.58000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.500                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 4EHR                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 34.71                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.88                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 17.5% PEG 8000, 0.2M NACL, 0.1M          
REMARK 280  PHOSPHATE CITRATE PH 4.0, VAPOR DIFFUSION, HANGING DROP,            
REMARK 280  TEMPERATURE 291K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       53.77000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       23.97500            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       53.77000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       23.97500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2080 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 7700 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -14.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A 415  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     GLU A    31                                                      
REMARK 465     GLU A    32                                                      
REMARK 465     ASN A    33                                                      
REMARK 465     ARG A    34                                                      
REMARK 465     THR A    35                                                      
REMARK 465     GLU A    36                                                      
REMARK 465     ALA A    37                                                      
REMARK 465     PRO A    38                                                      
REMARK 465     GLU A    39                                                      
REMARK 465     GLY A    40                                                      
REMARK 465     THR A    41                                                      
REMARK 465     GLU A    42                                                      
REMARK 465     SER A    43                                                      
REMARK 465     GLU A    44                                                      
REMARK 465     ASN A   197                                                      
REMARK 465     ASN A   198                                                      
REMARK 465     ALA A   199                                                      
REMARK 465     ALA A   200                                                      
REMARK 465     ALA A   201                                                      
REMARK 465     GLU A   202                                                      
REMARK 465     SER A   203                                                      
REMARK 465     ARG A   204                                                      
REMARK 465     LYS A   205                                                      
REMARK 465     GLY A   206                                                      
REMARK 465     GLN A   207                                                      
REMARK 465     GLU A   208                                                      
REMARK 465     ARG A   209                                                      
REMARK 465     ASP B    51                                                      
REMARK 465     MET B    52                                                      
REMARK 465     ARG B    53                                                      
REMARK 465     ARG B    75                                                      
REMARK 465     ARG B    76                                                      
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH A 342        DISTANCE =  6.20 ANGSTROMS                       
REMARK 525    HOH A 411        DISTANCE =  5.38 ANGSTROMS                       
REMARK 525    HOH A 419        DISTANCE =  7.02 ANGSTROMS                       
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1MAZ   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4QVE   RELATED DB: PDB                                   
DBREF  4QVF A    1   209  UNP    Q07817   B2CL1_HUMAN      1    209             
DBREF  4QVF B   51    76  UNP    O43521   B2L11_HUMAN    141    166             
SEQADV 4QVF     A       UNP  Q07817    MET    45 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    GLU    46 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    THR    47 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    PRO    48 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    SER    49 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ALA    50 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ILE    51 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ASN    52 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    GLY    53 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ASN    54 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    PRO    55 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    SER    56 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    TRP    57 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    HIS    58 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    LEU    59 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ALA    60 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ASP    61 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    SER    62 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    PRO    63 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ALA    64 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    VAL    65 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ASN    66 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    GLY    67 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ALA    68 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    THR    69 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    GLY    70 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    HIS    71 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    SER    72 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    SER    73 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    SER    74 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    LEU    75 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ASP    76 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ALA    77 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ARG    78 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    GLU    79 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    VAL    80 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ILE    81 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    PRO    82 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    MET    83 DELETION                       
SEQADV 4QVF     A       UNP  Q07817    ALA    84 DELETION                       
SEQRES   1 A  169  MET SER GLN SER ASN ARG GLU LEU VAL VAL ASP PHE LEU          
SEQRES   2 A  169  SER TYR LYS LEU SER GLN LYS GLY TYR SER TRP SER GLN          
SEQRES   3 A  169  PHE SER ASP VAL GLU GLU ASN ARG THR GLU ALA PRO GLU          
SEQRES   4 A  169  GLY THR GLU SER GLU ALA VAL LYS GLN ALA LEU ARG GLU          
SEQRES   5 A  169  ALA GLY ASP GLU PHE GLU LEU ARG TYR ARG ARG ALA PHE          
SEQRES   6 A  169  SER ASP LEU THR SER GLN LEU HIS ILE THR PRO GLY THR          
SEQRES   7 A  169  ALA TYR GLN SER PHE GLU GLN VAL VAL ASN GLU LEU PHE          
SEQRES   8 A  169  ARG ASP GLY VAL ASN TRP GLY ARG ILE VAL ALA PHE PHE          
SEQRES   9 A  169  SER PHE GLY GLY ALA LEU CYS VAL GLU SER VAL ASP LYS          
SEQRES  10 A  169  GLU MET GLN VAL LEU VAL SER ARG ILE ALA ALA TRP MET          
SEQRES  11 A  169  ALA THR TYR LEU ASN ASP HIS LEU GLU PRO TRP ILE GLN          
SEQRES  12 A  169  GLU ASN GLY GLY TRP ASP THR PHE VAL GLU LEU TYR GLY          
SEQRES  13 A  169  ASN ASN ALA ALA ALA GLU SER ARG LYS GLY GLN GLU ARG          
SEQRES   1 B   26  ASP MET ARG PRO GLU ILE TRP ILE ALA GLN GLU LEU ARG          
SEQRES   2 B   26  ARG ILE GLY ASP GLU PHE ASN ALA TYR TYR ALA ARG ARG          
FORMUL   3  HOH   *173(H2 O)                                                    
HELIX    1   1 SER A    4  GLN A   19  1                                  16    
HELIX    2   2 SER A   23  PHE A   27  5                                   5    
HELIX    3   3 VAL A   86  PHE A  105  1                                  20    
HELIX    4   4 ASP A  107  LEU A  112  1                                   6    
HELIX    5   5 THR A  118  PHE A  131  1                                  14    
HELIX    6   6 ASN A  136  LYS A  157  1                                  22    
HELIX    7   7 VAL A  161  LEU A  178  1                                  18    
HELIX    8   8 LEU A  178  ASN A  185  1                                   8    
HELIX    9   9 GLY A  187  GLY A  196  1                                  10    
HELIX   10  10 GLU B   55  ALA B   74  1                                  20    
CRYST1  107.540   47.950   34.180  90.00 105.03  90.00 C 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009299  0.000000  0.002497        0.00000                         
SCALE2      0.000000  0.020855  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.030293        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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