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Database: PDB
Entry: 4RG7
LinkDB: 4RG7
Original site: 4RG7 
HEADER    PROTEIN BINDING                         29-SEP-14   4RG7              
TITLE     CRYSTAL STRUCTURE OF APC3                                             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CELL DIVISION CYCLE PROTEIN 27 HOMOLOG;                    
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 1-180, 447-824;                               
COMPND   5 SYNONYM: ANAPHASE-PROMOTING COMPLEX SUBUNIT 3, APC3, CDC27 HOMOLOG,  
COMPND   6 CDC27HS, H-NUC;                                                      
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: CDC27, ANAPC3, D0S1430E, D17S978E;                             
SOURCE   6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA;                            
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 7108                                        
KEYWDS    SYMMETRIC HOMODIMER, TPR FPLDING, PROTEIN ASSEMBLY, PROTEIN BINDING   
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.YAMAGUCHI,S.YU,D.J.MILLER,B.A.SCHULMAN                              
REVDAT   4   02-AUG-17 4RG7    1       SOURCE REMARK                            
REVDAT   3   15-APR-15 4RG7    1       JRNL                                     
REVDAT   2   14-JAN-15 4RG7    1       JRNL                                     
REVDAT   1   24-DEC-14 4RG7    0                                                
JRNL        AUTH   M.YAMAGUCHI,S.YU,R.QIAO,F.WEISSMANN,D.J.MILLER,              
JRNL        AUTH 2 R.VANDERLINDEN,N.G.BROWN,J.J.FRYE,J.M.PETERS,B.A.SCHULMAN    
JRNL        TITL   STRUCTURE OF AN APC3-APC16 COMPLEX: INSIGHTS INTO ASSEMBLY   
JRNL        TITL 2 OF THE ANAPHASE-PROMOTING COMPLEX/CYCLOSOME.                 
JRNL        REF    J.MOL.BIOL.                   V. 427  1748 2015              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   25490258                                                     
JRNL        DOI    10.1016/J.JMB.2014.11.020                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    4.25 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8.4_1496)                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 4.25                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.67                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 15211                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.256                           
REMARK   3   R VALUE            (WORKING SET) : 0.255                           
REMARK   3   FREE R VALUE                     : 0.282                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.030                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 765                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 49.6690 -  7.2620    0.99     2923   134  0.1986 0.2136        
REMARK   3     2  7.2620 -  5.7669    1.00     2900   167  0.3008 0.3497        
REMARK   3     3  5.7669 -  5.0387    0.99     2874   149  0.3262 0.3367        
REMARK   3     4  5.0387 -  4.5784    1.00     2866   166  0.2992 0.3022        
REMARK   3     5  4.5784 -  4.2500    1.00     2883   149  0.3003 0.3415        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.680            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 33.310           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           6192                                  
REMARK   3   ANGLE     :  1.243           8529                                  
REMARK   3   CHIRALITY :  0.046           1005                                  
REMARK   3   PLANARITY :  0.005           1137                                  
REMARK   3   DIHEDRAL  : 13.409           1748                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 14                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 5:92 )                             
REMARK   3    ORIGIN FOR THE GROUP (A):  18.9730  18.1707 -18.3317              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.9909 T22:   1.4355                                     
REMARK   3      T33:   1.0277 T12:  -0.2181                                     
REMARK   3      T13:  -0.0981 T23:  -0.1437                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5986 L22:  10.1092                                     
REMARK   3      L33:   9.3187 L12:   0.6198                                     
REMARK   3      L13:  -0.2872 L23:   1.5676                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0357 S12:  -0.6877 S13:   1.2584                       
REMARK   3      S21:   0.1160 S22:   0.1915 S23:  -0.5612                       
REMARK   3      S31:   0.8194 S32:   1.1704 S33:  -0.3087                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 93:171 )                           
REMARK   3    ORIGIN FOR THE GROUP (A):   9.0705  -3.1276 -21.8179              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3106 T22:   1.2124                                     
REMARK   3      T33:   0.9199 T12:   0.0463                                     
REMARK   3      T13:   0.0037 T23:  -0.1617                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.1435 L22:   6.3800                                     
REMARK   3      L33:   7.5584 L12:  -1.2008                                     
REMARK   3      L13:  -1.0494 L23:  -1.0809                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0358 S12:   0.1597 S13:  -0.3243                       
REMARK   3      S21:  -0.9223 S22:  -0.0206 S23:  -0.3485                       
REMARK   3      S31:   0.2653 S32:   1.1120 S33:  -0.4042                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 464:504 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.0629  -9.6692  -4.4089              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3372 T22:   1.2754                                     
REMARK   3      T33:   1.2459 T12:   0.1467                                     
REMARK   3      T13:   0.2964 T23:  -0.0024                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.2941 L22:   7.1969                                     
REMARK   3      L33:   7.2185 L12:   1.4481                                     
REMARK   3      L13:  -2.6398 L23:   0.9975                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3784 S12:   0.3301 S13:   0.5967                       
REMARK   3      S21:  -0.8846 S22:  -0.6728 S23:  -0.9814                       
REMARK   3      S31:  -0.3904 S32:  -0.8378 S33:   0.2568                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 505:572 )                          
REMARK   3    ORIGIN FOR THE GROUP (A): -14.5655   5.2026  -3.1976              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0531 T22:   1.0281                                     
REMARK   3      T33:   0.9272 T12:   0.0466                                     
REMARK   3      T13:   0.0537 T23:  -0.1232                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.5531 L22:   4.4447                                     
REMARK   3      L33:   3.5675 L12:  -2.4516                                     
REMARK   3      L13:   3.6878 L23:  -4.1727                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0994 S12:  -0.6726 S13:   0.2779                       
REMARK   3      S21:   0.3700 S22:   0.1046 S23:   0.5595                       
REMARK   3      S31:   0.0832 S32:  -0.5166 S33:  -0.1094                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 573:687 )                          
REMARK   3    ORIGIN FOR THE GROUP (A): -20.6249   5.5922 -20.5426              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3035 T22:   1.1574                                     
REMARK   3      T33:   0.9295 T12:  -0.3538                                     
REMARK   3      T13:  -0.0687 T23:   0.0726                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.4924 L22:   2.6326                                     
REMARK   3      L33:   5.7668 L12:  -0.2521                                     
REMARK   3      L13:   3.2021 L23:   1.2426                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2839 S12:   0.7647 S13:   0.1003                       
REMARK   3      S21:   0.2387 S22:  -0.1806 S23:   0.3972                       
REMARK   3      S31:   1.2795 S32:  -1.0140 S33:  -0.0728                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 688:738 )                          
REMARK   3    ORIGIN FOR THE GROUP (A): -35.0659 -10.9288 -22.2270              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.4389 T22:   2.2784                                     
REMARK   3      T33:   2.8525 T12:  -0.8484                                     
REMARK   3      T13:  -0.6374 T23:   0.0087                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.6519 L22:   7.2905                                     
REMARK   3      L33:   7.3085 L12:  -5.8080                                     
REMARK   3      L13:   6.0934 L23:  -7.4498                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   3.5432 S12:  -1.8345 S13:  -0.6062                       
REMARK   3      S21:  -1.6216 S22:  -0.9139 S23:   4.6395                       
REMARK   3      S31:   3.2393 S32:  -3.8501 S33:  -1.9069                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 739:774 )                          
REMARK   3    ORIGIN FOR THE GROUP (A): -27.8214 -20.8828 -12.8248              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7300 T22:   1.5138                                     
REMARK   3      T33:   1.3607 T12:  -0.2239                                     
REMARK   3      T13:  -0.2751 T23:   0.0995                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.7553 L22:   7.2999                                     
REMARK   3      L33:   9.2696 L12:  -1.9091                                     
REMARK   3      L13:  -0.1702 L23:  -1.5345                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.3248 S12:  -0.2394 S13:   0.0804                       
REMARK   3      S21:  -0.4277 S22:   1.0114 S23:  -0.1912                       
REMARK   3      S31:   2.2878 S32:  -1.4215 S33:  -0.2133                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: ( CHAIN B AND RESID 6:81 )                             
REMARK   3    ORIGIN FOR THE GROUP (A):   8.5709   9.0826   0.1078              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1778 T22:   0.8597                                     
REMARK   3      T33:   1.0710 T12:   0.0674                                     
REMARK   3      T13:  -0.1766 T23:  -0.2282                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.4056 L22:   6.0153                                     
REMARK   3      L33:   6.5182 L12:  -0.8572                                     
REMARK   3      L13:  -3.3744 L23:   3.7138                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1036 S12:  -0.3126 S13:  -0.2250                       
REMARK   3      S21:  -0.5642 S22:  -0.1815 S23:   0.7407                       
REMARK   3      S31:  -0.7560 S32:   0.9144 S33:   0.3303                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: ( CHAIN B AND RESID 82:144 )                           
REMARK   3    ORIGIN FOR THE GROUP (A):  14.4369  28.8637   8.7961              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.3415 T22:   2.1223                                     
REMARK   3      T33:   1.3391 T12:  -0.8033                                     
REMARK   3      T13:   0.0023 T23:  -0.1820                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.8574 L22:   8.1682                                     
REMARK   3      L33:   4.4352 L12:   0.3575                                     
REMARK   3      L13:  -2.5757 L23:  -1.8479                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4444 S12:  -1.7529 S13:   1.5518                       
REMARK   3      S21:   1.1711 S22:   0.7863 S23:   0.2340                       
REMARK   3      S31:  -3.5112 S32:   2.9773 S33:  -0.4411                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: ( CHAIN B AND RESID 145:171 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):  24.8576  36.3088  -2.0344              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.5078 T22:   2.3266                                     
REMARK   3      T33:   1.2224 T12:  -0.4791                                     
REMARK   3      T13:   0.4336 T23:  -0.2566                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2833 L22:   2.6876                                     
REMARK   3      L33:   0.1217 L12:   0.8517                                     
REMARK   3      L13:  -0.4025 L23:  -0.7093                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1569 S12:   0.4349 S13:   0.7452                       
REMARK   3      S21:   0.9812 S22:  -0.6343 S23:  -0.6402                       
REMARK   3      S31:  -0.6503 S32:   0.4930 S33:   0.0419                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: ( CHAIN B AND RESID 465:534 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):  25.1566  41.0441 -17.4064              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7478 T22:   1.6781                                     
REMARK   3      T33:   1.4379 T12:  -0.6427                                     
REMARK   3      T13:  -0.0876 T23:  -0.0035                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.0465 L22:   4.7382                                     
REMARK   3      L33:   5.8406 L12:   0.7469                                     
REMARK   3      L13:  -0.8393 L23:  -0.6246                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0516 S12:   0.3744 S13:   0.9174                       
REMARK   3      S21:  -0.4565 S22:  -0.0592 S23:  -1.0112                       
REMARK   3      S31:  -1.1371 S32:   0.6589 S33:  -0.1098                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: ( CHAIN B AND RESID 535:686 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):   4.1035  46.7599 -17.0870              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.2136 T22:   1.4950                                     
REMARK   3      T33:   2.0810 T12:  -0.2831                                     
REMARK   3      T13:  -0.1952 T23:   0.2643                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9420 L22:   4.8074                                     
REMARK   3      L33:   5.6153 L12:  -2.0932                                     
REMARK   3      L13:  -0.5492 L23:   1.7924                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1477 S12:   0.1963 S13:   0.5746                       
REMARK   3      S21:  -1.0261 S22:   0.5408 S23:   1.7499                       
REMARK   3      S31:  -0.7949 S32:  -0.0361 S33:  -0.2773                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: ( CHAIN B AND RESID 687:724 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):   7.4688  69.5805 -13.8028              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.7614 T22:   1.6104                                     
REMARK   3      T33:   4.2326 T12:  -0.0823                                     
REMARK   3      T13:   0.0893 T23:   0.6045                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5064 L22:   2.6995                                     
REMARK   3      L33:   1.9789 L12:   0.0808                                     
REMARK   3      L13:  -3.5217 L23:  -4.8125                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   2.8246 S12:  -0.1376 S13:   2.9020                       
REMARK   3      S21:  -0.3736 S22:   1.3672 S23:   1.6579                       
REMARK   3      S31:  -4.9013 S32:  -2.3138 S33:  -3.4347                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: ( CHAIN B AND RESID 725:774 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):  21.6328  69.0848 -15.7528              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.6031 T22:   2.0423                                     
REMARK   3      T33:   3.0644 T12:  -0.5313                                     
REMARK   3      T13:   0.6656 T23:  -0.5473                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.6830 L22:   6.0750                                     
REMARK   3      L33:   9.0501 L12:   0.9102                                     
REMARK   3      L13:  -4.8218 L23:   0.1443                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   1.8980 S12:  -2.1850 S13:   2.7435                       
REMARK   3      S21:   1.0129 S22:   0.4252 S23:   1.2146                       
REMARK   3      S31:  -3.8705 S32:   2.4613 S33:  -1.2649                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4RG7 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 02-OCT-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000087306.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 14-FEB-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 24-ID-C                            
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97920                            
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : PSI PILATUS 6M                     
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 15458                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 4.250                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 5.800                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.11900                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 4.25                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 4.40                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : 0.82600                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 71.63                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.34                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M MES, PH 6.0, 0.2M MAGNESIUM         
REMARK 280  CHLORIDE, 8% PEG6000, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE    
REMARK 280  293K                                                                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 65                             
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   -X,-Y,Z+1/2                                             
REMARK 290       5555   Y,-X+Y,Z+1/6                                            
REMARK 290       6555   X-Y,X,Z+5/6                                             
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      182.49267            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       91.24633            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      136.86950            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       45.62317            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000      228.11583            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 4420 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 41940 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -24.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    -1                                                      
REMARK 465     SER A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     THR A     2                                                      
REMARK 465     VAL A     3                                                      
REMARK 465     LEU A     4                                                      
REMARK 465     SER A   172                                                      
REMARK 465     LEU A   173                                                      
REMARK 465     GLN A   174                                                      
REMARK 465     ASN A   175                                                      
REMARK 465     PHE A   176                                                      
REMARK 465     SER A   177                                                      
REMARK 465     ASN A   178                                                      
REMARK 465     CYS A   179                                                      
REMARK 465     LEU A   180                                                      
REMARK 465     PRO A   453                                                      
REMARK 465     GLN A   454                                                      
REMARK 465     ILE A   455                                                      
REMARK 465     GLN A   456                                                      
REMARK 465     ALA A   457                                                      
REMARK 465     PHE A   458                                                      
REMARK 465     ASN A   459                                                      
REMARK 465     LEU A   460                                                      
REMARK 465     GLN A   461                                                      
REMARK 465     LYS A   689                                                      
REMARK 465     LYS A   690                                                      
REMARK 465     SER A   691                                                      
REMARK 465     GLU A   692                                                      
REMARK 465     LYS A   693                                                      
REMARK 465     ALA A   694                                                      
REMARK 465     LEU A   695                                                      
REMARK 465     ASP A   696                                                      
REMARK 465     LYS A   775                                                      
REMARK 465     GLY A   776                                                      
REMARK 465     ALA A   777                                                      
REMARK 465     ASN A   778                                                      
REMARK 465     ASN A   779                                                      
REMARK 465     GLN A   780                                                      
REMARK 465     ILE A   781                                                      
REMARK 465     LYS A   782                                                      
REMARK 465     GLU A   783                                                      
REMARK 465     ALA A   784                                                      
REMARK 465     ILE A   785                                                      
REMARK 465     ASP A   786                                                      
REMARK 465     LYS A   787                                                      
REMARK 465     ARG A   788                                                      
REMARK 465     TYR A   789                                                      
REMARK 465     LEU A   790                                                      
REMARK 465     PRO A   791                                                      
REMARK 465     ASP A   792                                                      
REMARK 465     ASP A   793                                                      
REMARK 465     GLU A   794                                                      
REMARK 465     GLU A   795                                                      
REMARK 465     PRO A   796                                                      
REMARK 465     ILE A   797                                                      
REMARK 465     THR A   798                                                      
REMARK 465     GLN A   799                                                      
REMARK 465     GLU A   800                                                      
REMARK 465     GLU A   801                                                      
REMARK 465     GLN A   802                                                      
REMARK 465     ILE A   803                                                      
REMARK 465     MET A   804                                                      
REMARK 465     GLY A   805                                                      
REMARK 465     THR A   806                                                      
REMARK 465     ASP A   807                                                      
REMARK 465     GLU A   808                                                      
REMARK 465     SER A   809                                                      
REMARK 465     GLN A   810                                                      
REMARK 465     GLU A   811                                                      
REMARK 465     SER A   812                                                      
REMARK 465     SER A   813                                                      
REMARK 465     MET A   814                                                      
REMARK 465     THR A   815                                                      
REMARK 465     ASP A   816                                                      
REMARK 465     ALA A   817                                                      
REMARK 465     ASP A   818                                                      
REMARK 465     ASP A   819                                                      
REMARK 465     THR A   820                                                      
REMARK 465     GLN A   821                                                      
REMARK 465     LEU A   822                                                      
REMARK 465     HIS A   823                                                      
REMARK 465     ALA A   824                                                      
REMARK 465     ALA A   825                                                      
REMARK 465     GLU A   826                                                      
REMARK 465     SER A   827                                                      
REMARK 465     ASP A   828                                                      
REMARK 465     GLU A   829                                                      
REMARK 465     PHE A   830                                                      
REMARK 465     GLY B    -1                                                      
REMARK 465     SER B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     THR B     2                                                      
REMARK 465     VAL B     3                                                      
REMARK 465     LEU B     4                                                      
REMARK 465     GLN B     5                                                      
REMARK 465     SER B   172                                                      
REMARK 465     LEU B   173                                                      
REMARK 465     GLN B   174                                                      
REMARK 465     ASN B   175                                                      
REMARK 465     PHE B   176                                                      
REMARK 465     SER B   177                                                      
REMARK 465     ASN B   178                                                      
REMARK 465     CYS B   179                                                      
REMARK 465     LEU B   180                                                      
REMARK 465     PRO B   453                                                      
REMARK 465     GLN B   454                                                      
REMARK 465     ILE B   455                                                      
REMARK 465     GLN B   456                                                      
REMARK 465     ALA B   457                                                      
REMARK 465     PHE B   458                                                      
REMARK 465     ASN B   459                                                      
REMARK 465     LEU B   460                                                      
REMARK 465     GLN B   461                                                      
REMARK 465     LYS B   462                                                      
REMARK 465     ALA B   463                                                      
REMARK 465     ALA B   464                                                      
REMARK 465     LEU B   688                                                      
REMARK 465     LYS B   689                                                      
REMARK 465     LYS B   690                                                      
REMARK 465     SER B   691                                                      
REMARK 465     GLU B   692                                                      
REMARK 465     LYS B   693                                                      
REMARK 465     ALA B   694                                                      
REMARK 465     LEU B   695                                                      
REMARK 465     ASP B   696                                                      
REMARK 465     LYS B   775                                                      
REMARK 465     GLY B   776                                                      
REMARK 465     ALA B   777                                                      
REMARK 465     ASN B   778                                                      
REMARK 465     ASN B   779                                                      
REMARK 465     GLN B   780                                                      
REMARK 465     ILE B   781                                                      
REMARK 465     LYS B   782                                                      
REMARK 465     GLU B   783                                                      
REMARK 465     ALA B   784                                                      
REMARK 465     ILE B   785                                                      
REMARK 465     ASP B   786                                                      
REMARK 465     LYS B   787                                                      
REMARK 465     ARG B   788                                                      
REMARK 465     TYR B   789                                                      
REMARK 465     LEU B   790                                                      
REMARK 465     PRO B   791                                                      
REMARK 465     ASP B   792                                                      
REMARK 465     ASP B   793                                                      
REMARK 465     GLU B   794                                                      
REMARK 465     GLU B   795                                                      
REMARK 465     PRO B   796                                                      
REMARK 465     ILE B   797                                                      
REMARK 465     THR B   798                                                      
REMARK 465     GLN B   799                                                      
REMARK 465     GLU B   800                                                      
REMARK 465     GLU B   801                                                      
REMARK 465     GLN B   802                                                      
REMARK 465     ILE B   803                                                      
REMARK 465     MET B   804                                                      
REMARK 465     GLY B   805                                                      
REMARK 465     THR B   806                                                      
REMARK 465     ASP B   807                                                      
REMARK 465     GLU B   808                                                      
REMARK 465     SER B   809                                                      
REMARK 465     GLN B   810                                                      
REMARK 465     GLU B   811                                                      
REMARK 465     SER B   812                                                      
REMARK 465     SER B   813                                                      
REMARK 465     MET B   814                                                      
REMARK 465     THR B   815                                                      
REMARK 465     ASP B   816                                                      
REMARK 465     ALA B   817                                                      
REMARK 465     ASP B   818                                                      
REMARK 465     ASP B   819                                                      
REMARK 465     THR B   820                                                      
REMARK 465     GLN B   821                                                      
REMARK 465     LEU B   822                                                      
REMARK 465     HIS B   823                                                      
REMARK 465     ALA B   824                                                      
REMARK 465     ALA B   825                                                      
REMARK 465     GLU B   826                                                      
REMARK 465     SER B   827                                                      
REMARK 465     ASP B   828                                                      
REMARK 465     GLU B   829                                                      
REMARK 465     PHE B   830                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLN A   5    CG   CD   OE1  NE2                                  
REMARK 470     GLU A   6    CG   CD   OE1  OE2                                  
REMARK 470     VAL A   8    CG1  CG2                                            
REMARK 470     GLN A   9    CG   CD   OE1  NE2                                  
REMARK 470     GLN A  14    CG   CD   OE1  NE2                                  
REMARK 470     ASP A  23    CG   OD1  OD2                                       
REMARK 470     GLU A  38    CG   CD   OE1  OE2                                  
REMARK 470     GLU A  39    CG   CD   OE1  OE2                                  
REMARK 470     LEU A  41    CG   CD1  CD2                                       
REMARK 470     LEU A  43    CG   CD1  CD2                                       
REMARK 470     LYS A  53    CG   CD   CE   NZ                                   
REMARK 470     LYS A  56    CG   CD   CE   NZ                                   
REMARK 470     LEU A  60    CG   CD1  CD2                                       
REMARK 470     LEU A  61    CG   CD1  CD2                                       
REMARK 470     LYS A  62    CG   CD   CE   NZ                                   
REMARK 470     THR A  67    OG1  CG2                                            
REMARK 470     LYS A  72    CG   CD   CE   NZ                                   
REMARK 470     LEU A  75    CG   CD1  CD2                                       
REMARK 470     LYS A  84    CG   CD   CE   NZ                                   
REMARK 470     LEU A  85    CG   CD1  CD2                                       
REMARK 470     VAL A  96    CG1  CG2                                            
REMARK 470     PHE A  97    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ASN A  98    CG   OD1  ND2                                       
REMARK 470     LYS A  99    CG   CD   CE   NZ                                   
REMARK 470     GLN A 100    CG   CD   OE1  NE2                                  
REMARK 470     LYS A 101    CG   CD   CE   NZ                                   
REMARK 470     HIS A 103    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LYS A 127    CG   CD   CE   NZ                                   
REMARK 470     ARG A 130    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LEU A 131    CG   CD1  CD2                                       
REMARK 470     LYS A 133    CG   CD   CE   NZ                                   
REMARK 470     GLU A 136    CG   CD   OE1  OE2                                  
REMARK 470     GLN A 139    CG   CD   OE1  NE2                                  
REMARK 470     LYS A 140    CG   CD   CE   NZ                                   
REMARK 470     LEU A 142    CG   CD1  CD2                                       
REMARK 470     LEU A 144    CG   CD1  CD2                                       
REMARK 470     LEU A 148    CG   CD1  CD2                                       
REMARK 470     GLU A 160    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 161    CG   CD   CE   NZ                                   
REMARK 470     PRO A 162    CG   CD                                             
REMARK 470     ASP A 163    CG   OD1  OD2                                       
REMARK 470     GLN A 166    CG   CD   OE1  NE2                                  
REMARK 470     THR A 167    OG1  CG2                                            
REMARK 470     PHE A 168    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LYS A 169    CG   CD   CE   NZ                                   
REMARK 470     PHE A 170    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     THR A 171    OG1  CG2                                            
REMARK 470     LYS A 462    CG   CD   CE   NZ                                   
REMARK 470     GLU A 466    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 468    CG   CD1  CD2                                       
REMARK 470     MET A 469    CG   SD   CE                                        
REMARK 470     LEU A 471    CG   CD1  CD2                                       
REMARK 470     LEU A 472    CG   CD1  CD2                                       
REMARK 470     GLU A 474    CG   CD   OE1  OE2                                  
REMARK 470     MET A 475    CG   SD   CE                                        
REMARK 470     LYS A 477    CG   CD   CE   NZ                                   
REMARK 470     LEU A 482    CG   CD1  CD2                                       
REMARK 470     LYS A 488    CG   CD   CE   NZ                                   
REMARK 470     GLU A 489    CG   CD   OE1  OE2                                  
REMARK 470     ASN A 492    CG   OD1  ND2                                       
REMARK 470     LEU A 494    CG   CD1  CD2                                       
REMARK 470     HIS A 496    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU A 497    CG   CD1  CD2                                       
REMARK 470     ARG A 513    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 520    CG   CD   OE1  OE2                                  
REMARK 470     MET A 522    CG   SD   CE                                        
REMARK 470     GLN A 523    CG   CD   OE1  NE2                                  
REMARK 470     GLU A 525    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 526    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASN A 536    CG   OD1  ND2                                       
REMARK 470     GLU A 540    CG   CD   OE1  OE2                                  
REMARK 470     MET A 542    CG   SD   CE                                        
REMARK 470     LYS A 554    CG   CD   CE   NZ                                   
REMARK 470     LEU A 561    CG   CD1  CD2                                       
REMARK 470     LYS A 563    CG   CD   CE   NZ                                   
REMARK 470     MET A 568    CG   SD   CE                                        
REMARK 470     LYS A 570    CG   CD   CE   NZ                                   
REMARK 470     ASN A 571    CG   OD1  ND2                                       
REMARK 470     LEU A 585    CG   CD1  CD2                                       
REMARK 470     GLN A 586    CG   CD   OE1  NE2                                  
REMARK 470     ARG A 587    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 588    CG   CD   OE1  OE2                                  
REMARK 470     HIS A 589    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ILE A 591    CG1  CG2  CD1                                       
REMARK 470     ILE A 593    CG1  CG2  CD1                                       
REMARK 470     LYS A 594    CG   CD   CE   NZ                                   
REMARK 470     GLN A 597    CG   CD   OE1  NE2                                  
REMARK 470     ARG A 598    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLN A 601    CG   CD   OE1  NE2                                  
REMARK 470     ASN A 605    CG   OD1  ND2                                       
REMARK 470     LEU A 613    CG   CD1  CD2                                       
REMARK 470     GLU A 616    CG   CD   OE1  OE2                                  
REMARK 470     PHE A 617    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU A 619    CG   CD1  CD2                                       
REMARK 470     THR A 620    OG1  CG2                                            
REMARK 470     GLU A 621    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 622    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 623    CG   CD1  CD2                                       
REMARK 470     ASP A 624    CG   OD1  OD2                                       
REMARK 470     LYS A 625    CG   CD   CE   NZ                                   
REMARK 470     LEU A 627    CG   CD1  CD2                                       
REMARK 470     ARG A 631    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG A 639    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LEU A 647    CG   CD1  CD2                                       
REMARK 470     MET A 649    CG   SD   CE                                        
REMARK 470     ILE A 650    CG1  CG2  CD1                                       
REMARK 470     LYS A 653    CG   CD   CE   NZ                                   
REMARK 470     GLN A 654    CG   CD   OE1  NE2                                  
REMARK 470     GLU A 655    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 656    CG   CD   CE   NZ                                   
REMARK 470     PHE A 657    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU A 659    CG   CD1  CD2                                       
REMARK 470     GLU A 661    CG   CD   OE1  OE2                                  
REMARK 470     MET A 662    CG   SD   CE                                        
REMARK 470     GLN A 665    CG   CD   OE1  NE2                                  
REMARK 470     LYS A 666    CG   CD   CE   NZ                                   
REMARK 470     LEU A 668    CG   CD1  CD2                                       
REMARK 470     ILE A 670    CG1  CG2  CD1                                       
REMARK 470     ASN A 671    CG   OD1  ND2                                       
REMARK 470     GLN A 673    CG   CD   OE1  NE2                                  
REMARK 470     LEU A 677    CG   CD1  CD2                                       
REMARK 470     ILE A 681    CG1  CG2  CD1                                       
REMARK 470     VAL A 684    CG1  CG2                                            
REMARK 470     HIS A 686    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU A 688    CG   CD1  CD2                                       
REMARK 470     THR A 697    OG1  CG2                                            
REMARK 470     LEU A 698    CG   CD1  CD2                                       
REMARK 470     ASN A 699    CG   OD1  ND2                                       
REMARK 470     LYS A 700    CG   CD   CE   NZ                                   
REMARK 470     ILE A 702    CG1  CG2  CD1                                       
REMARK 470     VAL A 703    CG1  CG2                                            
REMARK 470     ILE A 704    CG1  CG2  CD1                                       
REMARK 470     ASP A 705    CG   OD1  OD2                                       
REMARK 470     PRO A 706    CG   CD                                             
REMARK 470     LYS A 707    CG   CD   CE   NZ                                   
REMARK 470     ASN A 708    CG   OD1  ND2                                       
REMARK 470     LYS A 712    CG   CD   CE   NZ                                   
REMARK 470     ARG A 715    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER A 717    OG                                                  
REMARK 470     VAL A 718    CG1  CG2                                            
REMARK 470     LEU A 719    CG   CD1  CD2                                       
REMARK 470     ASN A 722    CG   OD1  ND2                                       
REMARK 470     GLU A 723    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 724    CG   CD   CE   NZ                                   
REMARK 470     LYS A 726    CG   CD   CE   NZ                                   
REMARK 470     SER A 727    OG                                                  
REMARK 470     GLN A 730    CG   CD   OE1  NE2                                  
REMARK 470     GLU A 731    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 732    CG   CD1  CD2                                       
REMARK 470     GLU A 733    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 734    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 735    CG   CD1  CD2                                       
REMARK 470     LYS A 736    CG   CD   CE   NZ                                   
REMARK 470     GLN A 737    CG   CD   OE1  NE2                                  
REMARK 470     ILE A 738    CG1  CG2  CD1                                       
REMARK 470     VAL A 739    CG1  CG2                                            
REMARK 470     PRO A 740    CG   CD                                             
REMARK 470     LYS A 741    CG   CD   CE   NZ                                   
REMARK 470     GLU A 742    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 744    CG   CD1  CD2                                       
REMARK 470     VAL A 745    CG1  CG2                                            
REMARK 470     TYR A 746    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     PHE A 747    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU A 748    CG   CD1  CD2                                       
REMARK 470     ILE A 749    CG1  CG2  CD1                                       
REMARK 470     LYS A 751    CG   CD   CE   NZ                                   
REMARK 470     VAL A 752    CG1  CG2                                            
REMARK 470     TYR A 753    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LYS A 754    CG   CD   CE   NZ                                   
REMARK 470     LYS A 755    CG   CD   CE   NZ                                   
REMARK 470     LEU A 756    CG   CD1  CD2                                       
REMARK 470     GLN A 758    CG   CD   OE1  NE2                                  
REMARK 470     THR A 759    OG1  CG2                                            
REMARK 470     HIS A 760    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU A 761    CG   CD1  CD2                                       
REMARK 470     LEU A 763    CG   CD1  CD2                                       
REMARK 470     MET A 764    CG   SD   CE                                        
REMARK 470     ASN A 765    CG   OD1  ND2                                       
REMARK 470     PHE A 766    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     TRP A 768    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP A 768    CZ3  CH2                                            
REMARK 470     MET A 770    SD   CE                                             
REMARK 470     ASP A 771    CG   OD1  OD2                                       
REMARK 470     LEU A 772    CG   CD1  CD2                                       
REMARK 470     ASP A 773    CG   OD1  OD2                                       
REMARK 470     PRO A 774    CG   CD                                             
REMARK 470     GLN B   9    CG   CD   OE1  NE2                                  
REMARK 470     ARG B  22    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B  53    CG   CD   CE   NZ                                   
REMARK 470     LYS B  56    CG   CD   CE   NZ                                   
REMARK 470     ARG B  59    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B  62    CG   CD   CE   NZ                                   
REMARK 470     LYS B  72    CG   CD   CE   NZ                                   
REMARK 470     LEU B  75    CG   CD1  CD2                                       
REMARK 470     LYS B  77    CG   CD   CE   NZ                                   
REMARK 470     VAL B  80    CG1  CG2                                            
REMARK 470     LEU B  82    CG   CD1  CD2                                       
REMARK 470     LYS B  84    CG   CD   CE   NZ                                   
REMARK 470     LEU B  85    CG   CD1  CD2                                       
REMARK 470     GLU B  89    CG   CD   OE1  OE2                                  
REMARK 470     GLN B  90    CG   CD   OE1  NE2                                  
REMARK 470     LEU B  92    CG   CD1  CD2                                       
REMARK 470     PHE B  97    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ASN B  98    CG   OD1  ND2                                       
REMARK 470     LYS B  99    CG   CD   CE   NZ                                   
REMARK 470     LYS B 101    CG   CD   CE   NZ                                   
REMARK 470     ASP B 104    CG   OD1  OD2                                       
REMARK 470     VAL B 107    CG1  CG2                                            
REMARK 470     THR B 108    OG1  CG2                                            
REMARK 470     ASP B 112    CG   OD1  OD2                                       
REMARK 470     LYS B 127    CG   CD   CE   NZ                                   
REMARK 470     ASP B 129    CG   OD1  OD2                                       
REMARK 470     ARG B 130    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LEU B 131    CG   CD1  CD2                                       
REMARK 470     LYS B 133    CG   CD   CE   NZ                                   
REMARK 470     GLU B 136    CG   CD   OE1  OE2                                  
REMARK 470     GLN B 139    CG   CD   OE1  NE2                                  
REMARK 470     LYS B 140    CG   CD   CE   NZ                                   
REMARK 470     SER B 141    OG                                                  
REMARK 470     LEU B 142    CG   CD1  CD2                                       
REMARK 470     LEU B 144    CG   CD1  CD2                                       
REMARK 470     PHE B 147    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU B 148    CG   CD1  CD2                                       
REMARK 470     ILE B 158    CG1  CG2  CD1                                       
REMARK 470     GLU B 160    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 161    CG   CD   CE   NZ                                   
REMARK 470     PRO B 164    CG   CD                                             
REMARK 470     ASP B 165    CG   OD1  OD2                                       
REMARK 470     GLN B 166    CG   CD   OE1  NE2                                  
REMARK 470     LYS B 169    CG   CD   CE   NZ                                   
REMARK 470     PHE B 170    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     THR B 171    OG1  CG2                                            
REMARK 470     GLU B 466    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 468    CG   CD1  CD2                                       
REMARK 470     MET B 469    SD   CE                                             
REMARK 470     LEU B 471    CG   CD1  CD2                                       
REMARK 470     ARG B 473    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 474    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 477    CG   CD   CE   NZ                                   
REMARK 470     LEU B 482    CG   CD1  CD2                                       
REMARK 470     CYS B 487    SG                                                  
REMARK 470     LYS B 488    CG   CD   CE   NZ                                   
REMARK 470     GLU B 489    CG   CD   OE1  OE2                                  
REMARK 470     ILE B 491    CG1  CG2  CD1                                       
REMARK 470     ILE B 493    CG1  CG2  CD1                                       
REMARK 470     LEU B 494    CG   CD1  CD2                                       
REMARK 470     HIS B 496    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU B 497    CG   CD1  CD2                                       
REMARK 470     PRO B 498    CG   CD                                             
REMARK 470     ASN B 503    CG   OD1  ND2                                       
REMARK 470     LEU B 508    CG   CD1  CD2                                       
REMARK 470     ARG B 513    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     PHE B 516    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     GLU B 517    CG   CD   OE1  OE2                                  
REMARK 470     SER B 519    OG                                                  
REMARK 470     GLU B 520    CG   CD   OE1  OE2                                  
REMARK 470     TYR B 521    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     MET B 522    CG   SD   CE                                        
REMARK 470     GLU B 525    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 526    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE B 527    CG1  CG2  CD1                                       
REMARK 470     GLU B 530    CG   CD   OE1  OE2                                  
REMARK 470     VAL B 531    CG1  CG2                                            
REMARK 470     ARG B 532    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 533    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 540    CG   CD   OE1  OE2                                  
REMARK 470     MET B 542    CG   SD   CE                                        
REMARK 470     LEU B 549    CG   CD1  CD2                                       
REMARK 470     HIS B 551    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU B 552    CG   CD1  CD2                                       
REMARK 470     GLN B 553    CG   CD   OE1  NE2                                  
REMARK 470     LYS B 554    CG   CD   CE   NZ                                   
REMARK 470     ASP B 555    CG   OD1  OD2                                       
REMARK 470     LEU B 558    CG   CD1  CD2                                       
REMARK 470     SER B 559    OG                                                  
REMARK 470     LEU B 561    CG   CD1  CD2                                       
REMARK 470     LYS B 563    CG   CD   CE   NZ                                   
REMARK 470     LEU B 565    CG   CD1  CD2                                       
REMARK 470     ASP B 567    CG   OD1  OD2                                       
REMARK 470     MET B 568    CG   SD   CE                                        
REMARK 470     ASP B 569    CG   OD1  OD2                                       
REMARK 470     LYS B 570    CG   CD   CE   NZ                                   
REMARK 470     PRO B 573    CG   CD                                             
REMARK 470     CYS B 577    SG                                                  
REMARK 470     ASN B 581    CG   OD1  ND2                                       
REMARK 470     PHE B 583    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     GLN B 586    CG   CD   OE1  NE2                                  
REMARK 470     ARG B 587    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 588    CG   CD   OE1  OE2                                  
REMARK 470     HIS B 589    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ILE B 591    CG1  CG2  CD1                                       
REMARK 470     LYS B 594    CG   CD   CE   NZ                                   
REMARK 470     PHE B 595    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     PHE B 596    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ARG B 598    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE B 600    CG1  CG2  CD1                                       
REMARK 470     GLN B 601    CG   CD   OE1  NE2                                  
REMARK 470     VAL B 602    CG1  CG2                                            
REMARK 470     ASP B 603    CG   OD1  OD2                                       
REMARK 470     PRO B 604    CG   CD                                             
REMARK 470     ASN B 605    CG   OD1  ND2                                       
REMARK 470     TYR B 606    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LEU B 613    CG   CD1  CD2                                       
REMARK 470     HIS B 615    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU B 616    CG   CD   OE1  OE2                                  
REMARK 470     PHE B 617    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU B 619    CG   CD1  CD2                                       
REMARK 470     GLU B 621    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 622    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 623    CG   CD1  CD2                                       
REMARK 470     ASP B 624    CG   OD1  OD2                                       
REMARK 470     ARG B 631    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 635    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     VAL B 636    CG1  CG2                                            
REMARK 470     ASN B 637    CG   OD1  ND2                                       
REMARK 470     PRO B 638    CG   CD                                             
REMARK 470     ARG B 639    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     HIS B 640    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ASN B 642    CG   OD1  ND2                                       
REMARK 470     TYR B 645    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LEU B 647    CG   CD1  CD2                                       
REMARK 470     MET B 649    CG   SD   CE                                        
REMARK 470     ILE B 650    CG1  CG2  CD1                                       
REMARK 470     TYR B 651    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     TYR B 652    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LYS B 653    CG   CD   CE   NZ                                   
REMARK 470     GLN B 654    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 655    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 656    CG   CD   CE   NZ                                   
REMARK 470     PHE B 657    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU B 659    CG   CD1  CD2                                       
REMARK 470     GLU B 661    CG   CD   OE1  OE2                                  
REMARK 470     MET B 662    CG   SD   CE                                        
REMARK 470     HIS B 663    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LYS B 666    CG   CD   CE   NZ                                   
REMARK 470     ILE B 670    CG1  CG2  CD1                                       
REMARK 470     GLN B 673    CG   CD   OE1  NE2                                  
REMARK 470     LEU B 677    CG   CD1  CD2                                       
REMARK 470     LEU B 678    CG   CD1  CD2                                       
REMARK 470     ILE B 681    CG1  CG2  CD1                                       
REMARK 470     VAL B 683    CG1  CG2                                            
REMARK 470     VAL B 684    CG1  CG2                                            
REMARK 470     GLN B 685    CG   CD   OE1  NE2                                  
REMARK 470     HIS B 686    CG   ND1  CD2  CE1  NE2                             
REMARK 470     THR B 697    OG1  CG2                                            
REMARK 470     LEU B 698    CG   CD1  CD2                                       
REMARK 470     ASN B 699    CG   OD1  ND2                                       
REMARK 470     LYS B 700    CG   CD   CE   NZ                                   
REMARK 470     ILE B 702    CG1  CG2  CD1                                       
REMARK 470     VAL B 703    CG1  CG2                                            
REMARK 470     ILE B 704    CG1  CG2  CD1                                       
REMARK 470     ASP B 705    CG   OD1  OD2                                       
REMARK 470     PRO B 706    CG   CD                                             
REMARK 470     LYS B 707    CG   CD   CE   NZ                                   
REMARK 470     ASN B 708    CG   OD1  ND2                                       
REMARK 470     LEU B 710    CG   CD1  CD2                                       
REMARK 470     LYS B 712    CG   CD   CE   NZ                                   
REMARK 470     PHE B 713    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     HIS B 714    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ARG B 715    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     VAL B 718    CG1  CG2                                            
REMARK 470     LEU B 719    CG   CD1  CD2                                       
REMARK 470     ASN B 722    CG   OD1  ND2                                       
REMARK 470     GLU B 723    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 724    CG   CD   CE   NZ                                   
REMARK 470     LYS B 726    CG   CD   CE   NZ                                   
REMARK 470     LEU B 729    CG   CD1  CD2                                       
REMARK 470     GLN B 730    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 731    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 732    CG   CD1  CD2                                       
REMARK 470     GLU B 733    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 734    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 735    CG   CD1  CD2                                       
REMARK 470     LYS B 736    CG   CD   CE   NZ                                   
REMARK 470     GLN B 737    CG   CD   OE1  NE2                                  
REMARK 470     ILE B 738    CG1  CG2  CD1                                       
REMARK 470     VAL B 739    CG1  CG2                                            
REMARK 470     PRO B 740    CG   CD                                             
REMARK 470     LYS B 741    CG   CD   CE   NZ                                   
REMARK 470     GLU B 742    CG   CD   OE1  OE2                                  
REMARK 470     SER B 743    OG                                                  
REMARK 470     LEU B 744    CG   CD1  CD2                                       
REMARK 470     VAL B 745    CG1  CG2                                            
REMARK 470     TYR B 746    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     PHE B 747    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU B 748    CG   CD1  CD2                                       
REMARK 470     ILE B 749    CG1  CG2  CD1                                       
REMARK 470     LYS B 751    CG   CD   CE   NZ                                   
REMARK 470     VAL B 752    CG1  CG2                                            
REMARK 470     TYR B 753    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LYS B 754    CG   CD   CE   NZ                                   
REMARK 470     LYS B 755    CG   CD   CE   NZ                                   
REMARK 470     LEU B 756    CG   CD1  CD2                                       
REMARK 470     GLN B 758    CG   CD   OE1  NE2                                  
REMARK 470     THR B 759    OG1  CG2                                            
REMARK 470     HIS B 760    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU B 761    CG   CD1  CD2                                       
REMARK 470     LEU B 763    CG   CD1  CD2                                       
REMARK 470     MET B 764    CG   SD   CE                                        
REMARK 470     ASN B 765    CG   OD1  ND2                                       
REMARK 470     PHE B 766    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     SER B 767    OG                                                  
REMARK 470     TRP B 768    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP B 768    CZ3  CH2                                            
REMARK 470     MET B 770    CG   SD   CE                                        
REMARK 470     ASP B 771    CG   OD1  OD2                                       
REMARK 470     LEU B 772    CG   CD1  CD2                                       
REMARK 470     ASP B 773    CG   OD1  OD2                                       
REMARK 470     PRO B 774    CG   CD                                             
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OH   TYR A    32     NE2  HIS A    64              2.06            
REMARK 500   NE1  TRP A   550     OG   SER A   584              2.09            
REMARK 500   NE2  HIS A   501     OE1  GLU B    34              2.16            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    PRO A 709   C   -  N   -  CA  ANGL. DEV. =   9.6 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A  84       58.02    -99.14                                   
REMARK 500    PHE A  97      -78.96    -78.18                                   
REMARK 500    ASP A 129       36.37     70.14                                   
REMARK 500    TYR A 485       49.11    -78.74                                   
REMARK 500    SER A 519       24.70     82.89                                   
REMARK 500    ASN A 536       -8.30     69.44                                   
REMARK 500    ASN A 571       45.02   -103.64                                   
REMARK 500    GLN A 586       -3.75    -58.96                                   
REMARK 500    ASP A 603       96.12   -161.39                                   
REMARK 500    GLU A 622       47.70   -104.53                                   
REMARK 500    GLN A 673       30.38     76.01                                   
REMARK 500    LYS A 724       76.11   -104.53                                   
REMARK 500    GLN A 758       84.91    -65.28                                   
REMARK 500    LYS B  84       63.31   -118.57                                   
REMARK 500    ASN B  98      148.31    -38.23                                   
REMARK 500    ARG B 130       78.57   -119.79                                   
REMARK 500    GLN B 553       73.61   -101.81                                   
REMARK 500    LYS B 554       62.32   -113.43                                   
REMARK 500    GLN B 586        0.73    -67.35                                   
REMARK 500    ASN B 605       40.74   -103.31                                   
REMARK 500    GLU B 622       71.08   -111.92                                   
REMARK 500    ARG B 639       31.74    -90.23                                   
REMARK 500    ASN B 708       74.43   -115.32                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4RG6   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4RG9   RELATED DB: PDB                                   
DBREF  4RG7 A    1   180  UNP    P30260   CDC27_HUMAN      1    180             
DBREF  4RG7 A  453   830  UNP    P30260   CDC27_HUMAN    447    824             
DBREF  4RG7 B    1   180  UNP    P30260   CDC27_HUMAN      1    180             
DBREF  4RG7 B  453   830  UNP    P30260   CDC27_HUMAN    447    824             
SEQADV 4RG7 GLY A   -1  UNP  P30260              EXPRESSION TAG                 
SEQADV 4RG7 SER A    0  UNP  P30260              EXPRESSION TAG                 
SEQADV 4RG7 GLY B   -1  UNP  P30260              EXPRESSION TAG                 
SEQADV 4RG7 SER B    0  UNP  P30260              EXPRESSION TAG                 
SEQRES   1 A  560  GLY SER MET THR VAL LEU GLN GLU PRO VAL GLN ALA ALA          
SEQRES   2 A  560  ILE TRP GLN ALA LEU ASN HIS TYR ALA TYR ARG ASP ALA          
SEQRES   3 A  560  VAL PHE LEU ALA GLU ARG LEU TYR ALA GLU VAL HIS SER          
SEQRES   4 A  560  GLU GLU ALA LEU PHE LEU LEU ALA THR CYS TYR TYR ARG          
SEQRES   5 A  560  SER GLY LYS ALA TYR LYS ALA TYR ARG LEU LEU LYS GLY          
SEQRES   6 A  560  HIS SER CYS THR THR PRO GLN CYS LYS TYR LEU LEU ALA          
SEQRES   7 A  560  LYS CYS CYS VAL ASP LEU SER LYS LEU ALA GLU GLY GLU          
SEQRES   8 A  560  GLN ILE LEU SER GLY GLY VAL PHE ASN LYS GLN LYS SER          
SEQRES   9 A  560  HIS ASP ASP ILE VAL THR GLU PHE GLY ASP SER ALA CYS          
SEQRES  10 A  560  PHE THR LEU SER LEU LEU GLY HIS VAL TYR CYS LYS THR          
SEQRES  11 A  560  ASP ARG LEU ALA LYS GLY SER GLU CYS TYR GLN LYS SER          
SEQRES  12 A  560  LEU SER LEU ASN PRO PHE LEU TRP SER PRO PHE GLU SER          
SEQRES  13 A  560  LEU CYS GLU ILE GLY GLU LYS PRO ASP PRO ASP GLN THR          
SEQRES  14 A  560  PHE LYS PHE THR SER LEU GLN ASN PHE SER ASN CYS LEU          
SEQRES  15 A  560  PRO GLN ILE GLN ALA PHE ASN LEU GLN LYS ALA ALA ALA          
SEQRES  16 A  560  GLU GLY LEU MET SER LEU LEU ARG GLU MET GLY LYS GLY          
SEQRES  17 A  560  TYR LEU ALA LEU CYS SER TYR ASN CYS LYS GLU ALA ILE          
SEQRES  18 A  560  ASN ILE LEU SER HIS LEU PRO SER HIS HIS TYR ASN THR          
SEQRES  19 A  560  GLY TRP VAL LEU CYS GLN ILE GLY ARG ALA TYR PHE GLU          
SEQRES  20 A  560  LEU SER GLU TYR MET GLN ALA GLU ARG ILE PHE SER GLU          
SEQRES  21 A  560  VAL ARG ARG ILE GLU ASN TYR ARG VAL GLU GLY MET GLU          
SEQRES  22 A  560  ILE TYR SER THR THR LEU TRP HIS LEU GLN LYS ASP VAL          
SEQRES  23 A  560  ALA LEU SER VAL LEU SER LYS ASP LEU THR ASP MET ASP          
SEQRES  24 A  560  LYS ASN SER PRO GLU ALA TRP CYS ALA ALA GLY ASN CYS          
SEQRES  25 A  560  PHE SER LEU GLN ARG GLU HIS ASP ILE ALA ILE LYS PHE          
SEQRES  26 A  560  PHE GLN ARG ALA ILE GLN VAL ASP PRO ASN TYR ALA TYR          
SEQRES  27 A  560  ALA TYR THR LEU LEU GLY HIS GLU PHE VAL LEU THR GLU          
SEQRES  28 A  560  GLU LEU ASP LYS ALA LEU ALA CYS PHE ARG ASN ALA ILE          
SEQRES  29 A  560  ARG VAL ASN PRO ARG HIS TYR ASN ALA TRP TYR GLY LEU          
SEQRES  30 A  560  GLY MET ILE TYR TYR LYS GLN GLU LYS PHE SER LEU ALA          
SEQRES  31 A  560  GLU MET HIS PHE GLN LYS ALA LEU ASP ILE ASN PRO GLN          
SEQRES  32 A  560  SER SER VAL LEU LEU CYS HIS ILE GLY VAL VAL GLN HIS          
SEQRES  33 A  560  ALA LEU LYS LYS SER GLU LYS ALA LEU ASP THR LEU ASN          
SEQRES  34 A  560  LYS ALA ILE VAL ILE ASP PRO LYS ASN PRO LEU CYS LYS          
SEQRES  35 A  560  PHE HIS ARG ALA SER VAL LEU PHE ALA ASN GLU LYS TYR          
SEQRES  36 A  560  LYS SER ALA LEU GLN GLU LEU GLU GLU LEU LYS GLN ILE          
SEQRES  37 A  560  VAL PRO LYS GLU SER LEU VAL TYR PHE LEU ILE GLY LYS          
SEQRES  38 A  560  VAL TYR LYS LYS LEU GLY GLN THR HIS LEU ALA LEU MET          
SEQRES  39 A  560  ASN PHE SER TRP ALA MET ASP LEU ASP PRO LYS GLY ALA          
SEQRES  40 A  560  ASN ASN GLN ILE LYS GLU ALA ILE ASP LYS ARG TYR LEU          
SEQRES  41 A  560  PRO ASP ASP GLU GLU PRO ILE THR GLN GLU GLU GLN ILE          
SEQRES  42 A  560  MET GLY THR ASP GLU SER GLN GLU SER SER MET THR ASP          
SEQRES  43 A  560  ALA ASP ASP THR GLN LEU HIS ALA ALA GLU SER ASP GLU          
SEQRES  44 A  560  PHE                                                          
SEQRES   1 B  560  GLY SER MET THR VAL LEU GLN GLU PRO VAL GLN ALA ALA          
SEQRES   2 B  560  ILE TRP GLN ALA LEU ASN HIS TYR ALA TYR ARG ASP ALA          
SEQRES   3 B  560  VAL PHE LEU ALA GLU ARG LEU TYR ALA GLU VAL HIS SER          
SEQRES   4 B  560  GLU GLU ALA LEU PHE LEU LEU ALA THR CYS TYR TYR ARG          
SEQRES   5 B  560  SER GLY LYS ALA TYR LYS ALA TYR ARG LEU LEU LYS GLY          
SEQRES   6 B  560  HIS SER CYS THR THR PRO GLN CYS LYS TYR LEU LEU ALA          
SEQRES   7 B  560  LYS CYS CYS VAL ASP LEU SER LYS LEU ALA GLU GLY GLU          
SEQRES   8 B  560  GLN ILE LEU SER GLY GLY VAL PHE ASN LYS GLN LYS SER          
SEQRES   9 B  560  HIS ASP ASP ILE VAL THR GLU PHE GLY ASP SER ALA CYS          
SEQRES  10 B  560  PHE THR LEU SER LEU LEU GLY HIS VAL TYR CYS LYS THR          
SEQRES  11 B  560  ASP ARG LEU ALA LYS GLY SER GLU CYS TYR GLN LYS SER          
SEQRES  12 B  560  LEU SER LEU ASN PRO PHE LEU TRP SER PRO PHE GLU SER          
SEQRES  13 B  560  LEU CYS GLU ILE GLY GLU LYS PRO ASP PRO ASP GLN THR          
SEQRES  14 B  560  PHE LYS PHE THR SER LEU GLN ASN PHE SER ASN CYS LEU          
SEQRES  15 B  560  PRO GLN ILE GLN ALA PHE ASN LEU GLN LYS ALA ALA ALA          
SEQRES  16 B  560  GLU GLY LEU MET SER LEU LEU ARG GLU MET GLY LYS GLY          
SEQRES  17 B  560  TYR LEU ALA LEU CYS SER TYR ASN CYS LYS GLU ALA ILE          
SEQRES  18 B  560  ASN ILE LEU SER HIS LEU PRO SER HIS HIS TYR ASN THR          
SEQRES  19 B  560  GLY TRP VAL LEU CYS GLN ILE GLY ARG ALA TYR PHE GLU          
SEQRES  20 B  560  LEU SER GLU TYR MET GLN ALA GLU ARG ILE PHE SER GLU          
SEQRES  21 B  560  VAL ARG ARG ILE GLU ASN TYR ARG VAL GLU GLY MET GLU          
SEQRES  22 B  560  ILE TYR SER THR THR LEU TRP HIS LEU GLN LYS ASP VAL          
SEQRES  23 B  560  ALA LEU SER VAL LEU SER LYS ASP LEU THR ASP MET ASP          
SEQRES  24 B  560  LYS ASN SER PRO GLU ALA TRP CYS ALA ALA GLY ASN CYS          
SEQRES  25 B  560  PHE SER LEU GLN ARG GLU HIS ASP ILE ALA ILE LYS PHE          
SEQRES  26 B  560  PHE GLN ARG ALA ILE GLN VAL ASP PRO ASN TYR ALA TYR          
SEQRES  27 B  560  ALA TYR THR LEU LEU GLY HIS GLU PHE VAL LEU THR GLU          
SEQRES  28 B  560  GLU LEU ASP LYS ALA LEU ALA CYS PHE ARG ASN ALA ILE          
SEQRES  29 B  560  ARG VAL ASN PRO ARG HIS TYR ASN ALA TRP TYR GLY LEU          
SEQRES  30 B  560  GLY MET ILE TYR TYR LYS GLN GLU LYS PHE SER LEU ALA          
SEQRES  31 B  560  GLU MET HIS PHE GLN LYS ALA LEU ASP ILE ASN PRO GLN          
SEQRES  32 B  560  SER SER VAL LEU LEU CYS HIS ILE GLY VAL VAL GLN HIS          
SEQRES  33 B  560  ALA LEU LYS LYS SER GLU LYS ALA LEU ASP THR LEU ASN          
SEQRES  34 B  560  LYS ALA ILE VAL ILE ASP PRO LYS ASN PRO LEU CYS LYS          
SEQRES  35 B  560  PHE HIS ARG ALA SER VAL LEU PHE ALA ASN GLU LYS TYR          
SEQRES  36 B  560  LYS SER ALA LEU GLN GLU LEU GLU GLU LEU LYS GLN ILE          
SEQRES  37 B  560  VAL PRO LYS GLU SER LEU VAL TYR PHE LEU ILE GLY LYS          
SEQRES  38 B  560  VAL TYR LYS LYS LEU GLY GLN THR HIS LEU ALA LEU MET          
SEQRES  39 B  560  ASN PHE SER TRP ALA MET ASP LEU ASP PRO LYS GLY ALA          
SEQRES  40 B  560  ASN ASN GLN ILE LYS GLU ALA ILE ASP LYS ARG TYR LEU          
SEQRES  41 B  560  PRO ASP ASP GLU GLU PRO ILE THR GLN GLU GLU GLN ILE          
SEQRES  42 B  560  MET GLY THR ASP GLU SER GLN GLU SER SER MET THR ASP          
SEQRES  43 B  560  ALA ASP ASP THR GLN LEU HIS ALA ALA GLU SER ASP GLU          
SEQRES  44 B  560  PHE                                                          
HELIX    1   1 GLN A    5  HIS A   18  1                                  14    
HELIX    2   2 ALA A   20  VAL A   35  1                                  16    
HELIX    3   3 SER A   37  SER A   51  1                                  15    
HELIX    4   4 LYS A   53  HIS A   64  1                                  12    
HELIX    5   5 THR A   68  LEU A   82  1                                  15    
HELIX    6   6 LYS A   84  SER A   93  1                                  10    
HELIX    7   7 SER A  102  GLY A  111  1                                  10    
HELIX    8   8 SER A  113  THR A  128  1                                  16    
HELIX    9   9 ARG A  130  ASN A  145  1                                  16    
HELIX   10  10 LEU A  148  GLY A  159  1                                  12    
HELIX   11  11 ASP A  163  PHE A  168  1                                   6    
HELIX   12  12 ALA A  463  SER A  484  1                                  22    
HELIX   13  13 ASN A  486  LEU A  497  1                                  12    
HELIX   14  14 SER A  499  THR A  504  1                                   6    
HELIX   15  15 THR A  504  SER A  519  1                                  16    
HELIX   16  16 GLU A  520  GLU A  535  1                                  16    
HELIX   17  17 GLY A  541  GLN A  553  1                                  13    
HELIX   18  18 LYS A  554  ASP A  569  1                                  16    
HELIX   19  19 SER A  572  GLN A  586  1                                  15    
HELIX   20  20 GLU A  588  ASP A  603  1                                  16    
HELIX   21  21 TYR A  606  THR A  620  1                                  15    
HELIX   22  22 GLU A  622  ASN A  637  1                                  16    
HELIX   23  23 HIS A  640  GLN A  654  1                                  15    
HELIX   24  24 LYS A  656  ASN A  671  1                                  16    
HELIX   25  25 SER A  674  LEU A  688  1                                  15    
HELIX   26  26 LEU A  698  ASP A  705  1                                   8    
HELIX   27  27 ASN A  708  ASN A  722  1                                  15    
HELIX   28  28 LYS A  724  VAL A  739  1                                  16    
HELIX   29  29 GLU A  742  LYS A  755  1                                  14    
HELIX   30  30 GLN A  758  LEU A  772  1                                  15    
HELIX   31  31 PRO B    7  HIS B   18  1                                  12    
HELIX   32  32 ALA B   20  VAL B   35  1                                  16    
HELIX   33  33 SER B   37  SER B   51  1                                  15    
HELIX   34  34 LYS B   53  GLY B   63  1                                  11    
HELIX   35  35 THR B   68  LEU B   82  1                                  15    
HELIX   36  36 LYS B   84  SER B   93  1                                  10    
HELIX   37  37 SER B  102  GLY B  111  1                                  10    
HELIX   38  38 SER B  113  THR B  128  1                                  16    
HELIX   39  39 ARG B  130  ASN B  145  1                                  16    
HELIX   40  40 TRP B  149  ILE B  158  1                                  10    
HELIX   41  41 ASP B  163  PHE B  168  1                                   6    
HELIX   42  42 GLU B  466  SER B  484  1                                  19    
HELIX   43  43 ASN B  486  SER B  495  1                                  10    
HELIX   44  44 PRO B  498  ASN B  503  1                                   6    
HELIX   45  45 THR B  504  LEU B  518  1                                  15    
HELIX   46  46 GLU B  520  GLU B  535  1                                  16    
HELIX   47  47 MET B  542  LEU B  552  1                                  11    
HELIX   48  48 LYS B  554  ASP B  567  1                                  14    
HELIX   49  49 SER B  572  GLN B  586  1                                  15    
HELIX   50  50 GLU B  588  ASP B  603  1                                  16    
HELIX   51  51 TYR B  606  THR B  620  1                                  15    
HELIX   52  52 GLU B  622  ASN B  637  1                                  16    
HELIX   53  53 HIS B  640  GLN B  654  1                                  15    
HELIX   54  54 LYS B  656  ASN B  671  1                                  16    
HELIX   55  55 GLN B  673  ALA B  687  1                                  15    
HELIX   56  56 ASN B  699  ASN B  708  1                                  10    
HELIX   57  57 ASN B  708  ASN B  722  1                                  15    
HELIX   58  58 LYS B  724  VAL B  739  1                                  16    
HELIX   59  59 GLU B  742  LYS B  755  1                                  14    
HELIX   60  60 GLN B  758  ASP B  773  1                                  16    
CRYST1  118.408  118.408  273.739  90.00  90.00 120.00 P 65         12          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.008445  0.004876  0.000000        0.00000                         
SCALE2      0.000000  0.009752  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.003653        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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