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Database: PDB
Entry: 4U7I
LinkDB: 4U7I
Original site: 4U7I 
HEADER    PROTEIN TRANSPORT                       30-JUL-14   4U7I              
TITLE     STRUCTURE OF THE COMPLEX OF SPARTIN MIT AND IST1 MIM                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SPARTIN;                                                   
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: MIT DOMAIN (UNP RESIDUES 8-101);                           
COMPND   5 SYNONYM: SPASTIC PARAPLEGIA 20 PROTEIN,TRANS-ACTIVATED BY HEPATITIS C
COMPND   6 VIRUS CORE PROTEIN 1;                                                
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: IST1 HOMOLOG;                                              
COMPND  10 CHAIN: B;                                                            
COMPND  11 FRAGMENT: UNP RESIDUES 341-364;                                      
COMPND  12 SYNONYM: HIST1,PUTATIVE MAPK-ACTIVATING PROTEIN PM28;                
COMPND  13 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: SPG20, KIAA0610, TAHCCP1;                                      
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA (DE3);                             
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_COMMON: HUMAN;                                              
SOURCE  13 ORGANISM_TAXID: 9606;                                                
SOURCE  14 GENE: IST1, KIAA0174;                                                
SOURCE  15 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  16 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  17 EXPRESSION_SYSTEM_STRAIN: ROSETTA (DE3);                             
SOURCE  18 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID                               
KEYWDS    COMPLEX, MIM3, PROTEIN TRANSPORT                                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    E.Z.GUO,Z.XU                                                          
REVDAT   4   27-SEP-17 4U7I    1       SOURCE JRNL   REMARK                     
REVDAT   3   08-APR-15 4U7I    1       JRNL                                     
REVDAT   2   18-FEB-15 4U7I    1       JRNL                                     
REVDAT   1   11-FEB-15 4U7I    0                                                
JRNL        AUTH   E.Z.GUO,Z.XU                                                 
JRNL        TITL   DISTINCT MECHANISMS OF RECOGNIZING ENDOSOMAL SORTING COMPLEX 
JRNL        TITL 2 REQUIRED FOR TRANSPORT III (ESCRT-III) PROTEIN IST1 BY       
JRNL        TITL 3 DIFFERENT MICROTUBULE INTERACTING AND TRAFFICKING (MIT)      
JRNL        TITL 4 DOMAINS.                                                     
JRNL        REF    J.BIOL.CHEM.                  V. 290  8396 2015              
JRNL        REFN                   ESSN 1083-351X                               
JRNL        PMID   25657007                                                     
JRNL        DOI    10.1074/JBC.M114.607903                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.79 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: DEV_1593)                     
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.79                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 29.61                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 12400                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.192                           
REMARK   3   R VALUE            (WORKING SET) : 0.190                           
REMARK   3   FREE R VALUE                     : 0.220                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.820                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 598                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 29.6136 -  2.8467    0.99     3106   136  0.1806 0.2095        
REMARK   3     2  2.8467 -  2.2598    1.00     2936   157  0.2124 0.2286        
REMARK   3     3  2.2598 -  1.9742    1.00     2893   147  0.1919 0.2235        
REMARK   3     4  1.9742 -  1.7940    1.00     2867   158  0.1950 0.2728        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.190            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 23.940           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006            875                                  
REMARK   3   ANGLE     :  0.942           1170                                  
REMARK   3   CHIRALITY :  0.037            127                                  
REMARK   3   PLANARITY :  0.005            152                                  
REMARK   3   DIHEDRAL  : 13.703            337                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 1                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ALL                                                    
REMARK   3    ORIGIN FOR THE GROUP (A): -12.3844   6.1248  -7.1335              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1889 T22:   0.1303                                     
REMARK   3      T33:   0.2132 T12:  -0.0083                                     
REMARK   3      T13:  -0.0090 T23:   0.0173                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.8436 L22:   1.8780                                     
REMARK   3      L33:   5.5086 L12:   0.1574                                     
REMARK   3      L13:  -0.1277 L23:  -1.6184                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0032 S12:  -0.1122 S13:  -0.0740                       
REMARK   3      S21:   0.0177 S22:  -0.0414 S23:   0.0048                       
REMARK   3      S31:  -0.1073 S32:   0.1448 S33:   0.0114                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4U7I COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 04-AUG-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000202938.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 09-AUG-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 21-ID-F                            
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97828                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 225 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 12400                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.790                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 29.610                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.6                               
REMARK 200  DATA REDUNDANCY                : 8.100                              
REMARK 200  R MERGE                    (I) : 0.08100                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 43.0000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 8.40                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.58400                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 4.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 2DL1                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 45.10                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.24                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 2.9 M NA-MALONATE, PH 7.3, VAPOR         
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 277K                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+3/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+3/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       57.81000            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       23.12850            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       23.12850            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       86.71500            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       23.12850            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       23.12850            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       28.90500            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       23.12850            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       23.12850            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       86.71500            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       23.12850            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       23.12850            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       28.90500            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       57.81000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1480 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 6390 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -9.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A 246  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A     7                                                      
REMARK 465     GLY A     8                                                      
REMARK 465     SER B   312                                                      
REMARK 465     THR B   313                                                      
REMARK 465     SER B   314                                                      
REMARK 465     ALA B   315                                                      
REMARK 465     SER B   316                                                      
REMARK 465     GLU B   317                                                      
REMARK 465     LYS B   334                                                      
REMARK 465     THR B   335                                                      
REMARK 465     TRP B   336                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU A   9    CG   CD   OE1  OE2                                  
REMARK 470     GLU A  12    CD   OE1  OE2                                       
REMARK 470     LYS A  14    CE   NZ                                             
REMARK 470     ARG A  17    CZ   NH1  NH2                                       
REMARK 470     LYS A  21    NZ                                                  
REMARK 470     LYS A  22    NZ                                                  
REMARK 470     LYS A  39    NZ                                                  
REMARK 470     LYS A  47    NZ                                                  
REMARK 470     SER A  64    OG                                                  
REMARK 470     GLU A  65    CG   CD   OE1  OE2                                  
REMARK 470     LYS A  97    NZ                                                  
REMARK 470     LYS B 333    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HOH A   226     O    HOH A   233              2.09            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    SER A  61       15.11   -142.08                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4U7E   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4U7Y   RELATED DB: PDB                                   
DBREF  4U7I A    8   101  UNP    Q8N0X7   SPG20_HUMAN      8    101             
DBREF  4U7I B  312   335  UNP    P53990   IST1_HUMAN     341    364             
SEQADV 4U7I SER A    7  UNP  Q8N0X7              EXPRESSION TAG                 
SEQADV 4U7I TRP B  336  UNP  P53990              EXPRESSION TAG                 
SEQRES   1 A   95  SER GLY GLU PRO ALA GLU ILE LYS ILE ILE ARG GLU ALA          
SEQRES   2 A   95  TYR LYS LYS ALA PHE LEU PHE VAL ASN LYS GLY LEU ASN          
SEQRES   3 A   95  THR ASP GLU LEU GLY GLN LYS GLU GLU ALA LYS ASN TYR          
SEQRES   4 A   95  TYR LYS GLN GLY ILE GLY HIS LEU LEU ARG GLY ILE SER          
SEQRES   5 A   95  ILE SER SER LYS GLU SER GLU HIS THR GLY PRO GLY TRP          
SEQRES   6 A   95  GLU SER ALA ARG GLN MET GLN GLN LYS MET LYS GLU THR          
SEQRES   7 A   95  LEU GLN ASN VAL ARG THR ARG LEU GLU ILE LEU GLU LYS          
SEQRES   8 A   95  GLY LEU ALA THR                                              
SEQRES   1 B   25  SER THR SER ALA SER GLU ASP ILE ASP PHE ASP ASP LEU          
SEQRES   2 B   25  SER ARG ARG PHE GLU GLU LEU LYS LYS LYS THR TRP              
FORMUL   3  HOH   *100(H2 O)                                                    
HELIX    1 AA1 PRO A   10  LEU A   36  1                                  27    
HELIX    2 AA2 GLN A   38  SER A   58  1                                  21    
HELIX    3 AA3 GLY A   68  LYS A   97  1                                  30    
HELIX    4 AA4 ASP B  320  LYS B  333  1                                  14    
CRYST1   46.257   46.257  115.620  90.00  90.00  90.00 P 43 21 2     8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.021618  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.021618  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.008649        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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