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Database: PDB
Entry: 4UF2
LinkDB: 4UF2
Original site: 4UF2 
HEADER    VIRAL PROTEIN                           23-DEC-14   4UF2              
TITLE     DEERPOX VIRUS DPV022 IN COMPLEX WITH BAX BH3                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ANTIAPOPTOTIC MEMBRANE PROTEIN;                            
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: BCL-2, UNP RESIDUES 1-155;                                 
COMPND   5 SYNONYM: DPV022;                                                     
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: APOPTOSIS REGULATOR BAX;                                   
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: BH3, UNP RESIDUES 50-77;                                   
COMPND  11 SYNONYM: BCL-2-LIKE PROTEIN 4, BCL2-L-4, BAX                         
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MULE DEER POXVIRUS;                             
SOURCE   3 ORGANISM_TAXID: 304399;                                              
SOURCE   4 STRAIN: W-848-83;                                                    
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PDUET;                                    
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 SYNTHETIC: YES;                                                      
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606                                                 
KEYWDS    VIRAL PROTEIN, DEERPOX VIRUS, APOPTOSIS, BCL-2, BAX BH3               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    D.R.BURTON,M.KVANSAKUL                                                
REVDAT   3   19-AUG-15 4UF2    1       JRNL                                     
REVDAT   2   12-AUG-15 4UF2    1       JRNL                                     
REVDAT   1   05-AUG-15 4UF2    0                                                
JRNL        AUTH   D.R.BURTON,S.CARIA,B.MARSHALL,M.BARRY,M.KVANSAKUL            
JRNL        TITL   STRUCTURAL BASIS OF DEERPOX VIRUS-MEDIATED INHIBITION OF     
JRNL        TITL 2 APOPTOSIS.                                                   
JRNL        REF    ACTA CRYSTALLOGR.,SECT.D      V.  71  1593 2015              
JRNL        REFN                   ISSN 0907-4449                               
JRNL        PMID   26249341                                                     
JRNL        DOI    10.1107/S1399004715009402                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE)                               
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.000                          
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 46.240                         
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.35                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.93                          
REMARK   3   NUMBER OF REFLECTIONS             : 4265                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.2167                          
REMARK   3   R VALUE            (WORKING SET) : 0.2142                          
REMARK   3   FREE R VALUE                     : 0.2743                          
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.6                             
REMARK   3   FREE R VALUE TEST SET COUNT      : 194                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE  CCWORK
REMARK   3     1 46.2460 -  3.0005    1.00     4071   194  0.2142 0.2743   0.952
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.03             
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.61            
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 76.19                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 81.6                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.002           1272                                  
REMARK   3   ANGLE     :  0.403           1704                                  
REMARK   3   CHIRALITY :  0.017            199                                  
REMARK   3   PLANARITY :  0.001            213                                  
REMARK   3   DIHEDRAL  :  9.348            497                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4UF2 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 23-DEC-14.                  
REMARK 100 THE PDBE ID CODE IS EBI-62633.                                       
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 09-OCT-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON             
REMARK 200  BEAMLINE                       : MX2                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.935370                           
REMARK 200  MONOCHROMATOR                  : DOUBLE CRYSTAL                     
REMARK 200                                   MONOCHROMATOR (SI111)              
REMARK 200  OPTICS                         : SILICON MIRRORS (ADAPTIVE          
REMARK 200                                   AND U-BENT)                        
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD (QUANTUM 315R)                 
REMARK 200  DETECTOR MANUFACTURER          : ADSC                               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS                            
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 4297                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.00                               
REMARK 200  RESOLUTION RANGE LOW       (A) : 92.48                              
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.0                                
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 6.9                                
REMARK 200  R MERGE                    (I) : 0.14                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 10.20                              
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.00                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.18                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.2                                
REMARK 200  R MERGE FOR SHELL          (I) : 1.10                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.70                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: DPV022_BIM                                           
REMARK 200                                                                      
REMARK 200 REMARK: RESIDUES FROM 1 AND 2 AND FROM 138 TO 155 ARE                
REMARK 200  DISORDERED IN DPV022 AND IN BAX BH3 THE RESIDUES FROM 51            
REMARK 200  TO 56 AND 76 TO 78 ARE MISSING.                                     
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 81.6                                      
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.99                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 20% PEG 6000, 0.2M MES PH 6.0,           
REMARK 280  0,2M AMMONIUM SULPHATE.                                             
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 41 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+1/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+3/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+1/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+3/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       22.76450            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       46.24050            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       46.24050            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       11.38225            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       46.24050            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       46.24050            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       34.14675            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       46.24050            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       46.24050            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       11.38225            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       46.24050            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       46.24050            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       34.14675            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       22.76450            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 8610 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15390 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -62.7 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       22.76450            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   -12                                                      
REMARK 465     GLY A   -11                                                      
REMARK 465     SER A   -10                                                      
REMARK 465     SER A    -9                                                      
REMARK 465     HIS A    -8                                                      
REMARK 465     HIS A    -7                                                      
REMARK 465     HIS A    -6                                                      
REMARK 465     HIS A    -5                                                      
REMARK 465     HIS A    -4                                                      
REMARK 465     HIS A    -3                                                      
REMARK 465     SER A    -2                                                      
REMARK 465     GLN A    -1                                                      
REMARK 465     ASP A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     GLU A     2                                                      
REMARK 465     ASP A   139                                                      
REMARK 465     ASP A   140                                                      
REMARK 465     ASP A   141                                                      
REMARK 465     ASP A   142                                                      
REMARK 465     ILE A   143                                                      
REMARK 465     VAL A   144                                                      
REMARK 465     ASP A   145                                                      
REMARK 465     ASP A   146                                                      
REMARK 465     TYR A   147                                                      
REMARK 465     VAL A   148                                                      
REMARK 465     LEU A   149                                                      
REMARK 465     ILE A   150                                                      
REMARK 465     THR A   151                                                      
REMARK 465     ASN A   152                                                      
REMARK 465     TYR A   153                                                      
REMARK 465     LEU A   154                                                      
REMARK 465     LYS A   155                                                      
REMARK 465     VAL B     2                                                      
REMARK 465     PRO B     3                                                      
REMARK 465     GLN B     4                                                      
REMARK 465     ASP B     5                                                      
REMARK 465     ALA B     6                                                      
REMARK 465     SER B     7                                                      
REMARK 465     GLU B    27                                                      
REMARK 465     LEU B    28                                                      
REMARK 465     GLN B    29                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ALA A   4       89.40    -67.23                                   
REMARK 500    LEU A  30       38.81    -95.97                                   
REMARK 500    ASN A  65      -78.81    -76.18                                   
REMARK 500    SER A  72      -32.53     63.04                                   
REMARK 500    SER A 118     -173.14    -68.18                                   
REMARK 500    ASN A 119     -128.53     59.40                                   
REMARK 500    LYS B   9       80.18     54.86                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4UF1   RELATED DB: PDB                                   
REMARK 900  DEERPOX VIRUS DPV022 IN COMPLEX WITH BAK BH3                        
REMARK 900 RELATED ID: 4UF3   RELATED DB: PDB                                   
REMARK 900  DEERPOX VIRUS DPV022 IN COMPLEX WITH BIM BH3                        
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999 STRUCTURE FROM CONSTRUCT RESIDUES 1-155                              
DBREF  4UF2 A    1   155  UNP    Q08FF8   Q08FF8_DPV84     1    155             
DBREF  4UF2 B    2    29  UNP    Q07812   BAX_HUMAN       50     77             
SEQADV 4UF2 MET A  -12  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 GLY A  -11  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 SER A  -10  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 SER A   -9  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 HIS A   -8  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 HIS A   -7  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 HIS A   -6  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 HIS A   -5  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 HIS A   -4  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 HIS A   -3  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 SER A   -2  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 GLN A   -1  UNP  Q08FF8              EXPRESSION TAG                 
SEQADV 4UF2 ASP A    0  UNP  Q08FF8              EXPRESSION TAG                 
SEQRES   1 A  168  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER GLN ASP          
SEQRES   2 A  168  MET GLU ALA ALA ILE GLU PHE ASP GLU ILE VAL LYS LYS          
SEQRES   3 A  168  LEU LEU ASN ILE TYR ILE ASN ASP ILE CYS THR THR GLY          
SEQRES   4 A  168  GLU LYS ARG LEU LEU ASN ASN TYR GLU LYS SER ILE LEU          
SEQRES   5 A  168  ASP ARG ILE TYR LYS SER CYS GLU TYR ILE LYS LYS ASN          
SEQRES   6 A  168  TYR GLU LEU ASP PHE ASN SER MET TYR ASN GLN ILE ASN          
SEQRES   7 A  168  ILE ASN ASP ILE THR THR SER ASP ILE LYS SER LYS ILE          
SEQRES   8 A  168  ILE GLU ALA LEU LEU ILE ASP SER ARG PRO SER VAL LYS          
SEQRES   9 A  168  LEU ALA THR LEU SER PHE ILE SER LEU ILE ALA GLU LYS          
SEQRES  10 A  168  TRP GLY GLU LYS ASN ARG ALA LYS ILE MET GLU ILE LEU          
SEQRES  11 A  168  SER ASN GLU ILE VAL GLU LYS ILE SER ASN ASN GLY LYS          
SEQRES  12 A  168  ASP PHE ILE ASP PHE ILE ASP ARG ASP ASP ASP ASP ILE          
SEQRES  13 A  168  VAL ASP ASP TYR VAL LEU ILE THR ASN TYR LEU LYS              
SEQRES   1 B   28  VAL PRO GLN ASP ALA SER THR LYS LYS LEU SER GLU CYS          
SEQRES   2 B   28  LEU LYS ARG ILE GLY ASP GLU LEU ASP SER ASN MET GLU          
SEQRES   3 B   28  LEU GLN                                                      
HELIX    1   1 PHE A    7  LEU A   30  1                                  24    
HELIX    2   2 ASN A   32  TYR A   53  1                                  22    
HELIX    3   3 TYR A   53  ASN A   65  1                                  13    
HELIX    4   4 SER A   72  ASP A   85  1                                  14    
HELIX    5   5 ARG A   87  TRP A  105  1                                  19    
HELIX    6   6 ARG A  110  SER A  118  1                                   9    
HELIX    7   7 ASN A  119  ASN A  127  1                                   9    
HELIX    8   8 ASN A  128  ASP A  137  1                                  10    
HELIX    9   9 LYS B   10  SER B   24  1                                  15    
CRYST1   92.481   92.481   45.529  90.00  90.00  90.00 P 41 21 2     8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.010813  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.010813  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.021964        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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