HEADER TRANSPORT PROTEIN 27-AUG-14 4UXR
TITLE CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE, ATP
TITLE 2 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: TUBULIN ALPHA-1B CHAIN;
COMPND 3 CHAIN: A;
COMPND 4 SYNONYM: ALPHA-TUBULIN UBIQUITOUS;
COMPND 5 MOL_ID: 2;
COMPND 6 MOLECULE: TUBULIN BETA-2B CHAIN;
COMPND 7 CHAIN: B;
COMPND 8 MOL_ID: 3;
COMPND 9 MOLECULE: KINESIN-3 MOTOR DOMAIN;
COMPND 10 CHAIN: C;
COMPND 11 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: BOS TAURUS;
SOURCE 3 ORGANISM_COMMON: CATTLE;
SOURCE 4 ORGANISM_TAXID: 9913;
SOURCE 5 MOL_ID: 2;
SOURCE 6 ORGANISM_SCIENTIFIC: BOS TAURUS;
SOURCE 7 ORGANISM_COMMON: CATTLE;
SOURCE 8 ORGANISM_TAXID: 9913;
SOURCE 9 MOL_ID: 3;
SOURCE 10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 11 ORGANISM_COMMON: HUMAN;
SOURCE 12 ORGANISM_TAXID: 9606;
SOURCE 13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 14 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 15 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 16 EXPRESSION_SYSTEM_PLASMID: PFN18A
KEYWDS TRANSPORT PROTEIN, MICROTUBULE
EXPDTA ELECTRON MICROSCOPY
MDLTYP CA ATOMS ONLY, CHAIN A, B, C
AUTHOR J.ATHERTON,I.FARABELLA,I.M.YU,S.S.ROSENFELD,A.HOUDUSSE,M.TOPF,
AUTHOR 2 C.MOORES
REVDAT 3 23-AUG-17 4UXR 1 REMARK
REVDAT 2 22-OCT-14 4UXR 1 JRNL
REVDAT 1 24-SEP-14 4UXR 0
JRNL AUTH J.ATHERTON,I.FARABELLA,I.YU,S.S.ROSENFELD,A.HOUDUSSE,M.TOPF,
JRNL AUTH 2 C.A.MOORES
JRNL TITL CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE,
JRNL TITL 2 ATP BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS.
JRNL REF ELIFE V. 3 3680 2014
JRNL REFN ESSN 2050-084X
JRNL PMID 25209998
JRNL DOI 10.7554/ELIFE.03680
REMARK 2
REMARK 2 RESOLUTION. 7.00 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 SOFTWARE PACKAGES : FLEX-EM, UCSF CHIMERA, FREALIGN, SPIDER
REMARK 3 RECONSTRUCTION SCHEMA : NULL
REMARK 3
REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT
REMARK 3 PDB ENTRY : 1VFV
REMARK 3 REFINEMENT SPACE : REAL
REMARK 3 REFINEMENT PROTOCOL : FLEXIBLE FIT
REMARK 3 REFINEMENT TARGET : CROSS-CORRELATION COEFFICIENT
REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL
REMARK 3
REMARK 3 FITTING PROCEDURE : METHOD--FLEXIBLE REFINEMENT PROTOCOL--X-RAY
REMARK 3
REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS
REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL
REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 7.000
REMARK 3 NUMBER OF PARTICLES : 156845
REMARK 3 CTF CORRECTION METHOD : NULL
REMARK 3
REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL
REMARK 3
REMARK 3 OTHER DETAILS: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD
REMARK 3 -2767. (DEPOSITION ID: 12600).
REMARK 4
REMARK 4 4UXR COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE.
REMARK 100 THE DEPOSITION ID IS D_1290061615.
REMARK 245
REMARK 245 EXPERIMENTAL DETAILS
REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE
REMARK 245 SPECIMEN TYPE : VITREOUS ICE
REMARK 245
REMARK 245 ELECTRON MICROSCOPE SAMPLE
REMARK 245 SAMPLE TYPE : HELICAL ARRAY
REMARK 245 PARTICLE TYPE : POINT
REMARK 245 NAME OF SAMPLE : 13-PROTOFILAMENT MICROTUBULE
REMARK 245 -BOUND HUMAN KINESIN-3 MOTOR
REMARK 245 DOMAIN WITH ADPALFX
REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL
REMARK 245 SAMPLE SUPPORT DETAILS : NULL
REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL
REMARK 245 SAMPLE BUFFER : NULL
REMARK 245 PH : 6.80
REMARK 245 SAMPLE DETAILS : NULL
REMARK 245
REMARK 245 DATA ACQUISITION
REMARK 245 DATE OF EXPERIMENT : 10-DEC-12
REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL
REMARK 245 TEMPERATURE (KELVIN) : 90.00
REMARK 245 MICROSCOPE MODEL : FEI POLARA 300
REMARK 245 DETECTOR TYPE : GATAN ULTRASCAN 4000 (4K X
REMARK 245 4K)
REMARK 245 MINIMUM DEFOCUS (NM) : 400.00
REMARK 245 MAXIMUM DEFOCUS (NM) : 3500.00
REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL
REMARK 245 NOMINAL CS : 2.30
REMARK 245 IMAGING MODE : BRIGHT FIELD
REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 20.00
REMARK 245 ILLUMINATION MODE : FLOOD BEAM
REMARK 245 NOMINAL MAGNIFICATION : NULL
REMARK 245 CALIBRATED MAGNIFICATION : 100000
REMARK 245 SOURCE : FIELD EMISSION GUN
REMARK 245 ACCELERATION VOLTAGE (KV) : 300
REMARK 245 IMAGING DETAILS : NULL
REMARK 247
REMARK 247 ELECTRON MICROSCOPY
REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON
REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE
REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES
REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION
REMARK 247 OF THE STRUCTURE FACTORS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 MET A 0
REMARK 465 GLN A 33A
REMARK 465 MET A 33B
REMARK 465 PRO A 33C
REMARK 465 SER A 33D
REMARK 465 ASP A 33E
REMARK 465 LYS A 33F
REMARK 465 THR A 33G
REMARK 465 ILE A 33H
REMARK 465 GLY A 33I
REMARK 465 GLY A 33J
REMARK 465 GLY A 33K
REMARK 465 ASP A 33L
REMARK 465 ASP A 33M
REMARK 465 SER A 33N
REMARK 465 PHE A 33O
REMARK 465 ASN A 33P
REMARK 465 THR A 33Q
REMARK 465 PHE A 33R
REMARK 465 PHE A 33S
REMARK 465 SER A 33T
REMARK 465 GLU A 33U
REMARK 465 THR A 33V
REMARK 465 GLY A 33W
REMARK 465 ALA A 33X
REMARK 465 GLY A 33Y
REMARK 465 LYS A 33Z
REMARK 465 VAL A 413
REMARK 465 GLU A 414
REMARK 465 GLY A 415
REMARK 465 GLU A 416
REMARK 465 GLY A 417
REMARK 465 GLU A 418
REMARK 465 GLU A 419
REMARK 465 GLU A 420
REMARK 465 GLY A 421
REMARK 465 GLU A 422
REMARK 465 GLU A 423
REMARK 465 TYR A 424
REMARK 465 MET B 0
REMARK 465 ALA B 427
REMARK 465 THR B 428
REMARK 465 ALA B 429
REMARK 465 ASP B 430
REMARK 465 GLU B 431
REMARK 465 GLN B 432
REMARK 465 GLY B 433
REMARK 465 GLU B 434
REMARK 465 PHE B 435
REMARK 465 GLU B 436
REMARK 465 GLU B 437
REMARK 465 GLU B 438
REMARK 465 GLY B 439
REMARK 465 GLU B 440
REMARK 465 GLU B 441
REMARK 465 ASP B 442
REMARK 465 GLU B 443
REMARK 465 ALA B 444
REMARK 465 SER C -5
REMARK 465 ASP C -4
REMARK 465 ASN C -3
REMARK 465 ALA C -2
REMARK 465 ILE C -1
REMARK 465 ALA C 0
REMARK 465 LYS C 294
REMARK 465 ASN C 295
REMARK 465 LYS C 296
REMARK 465 LYS C 297
REMARK 465 LYS C 298
REMARK 465 LYS C 299
REMARK 465 LYS C 300
REMARK 465 THR C 301
REMARK 465 ASN C 361
REMARK 465 HIS C 362
REMARK 465 HIS C 363
REMARK 465 HIS C 364
REMARK 465 HIS C 365
REMARK 465 HIS C 366
REMARK 465 HIS C 367
REMARK 465 HIS C 368
REMARK 465 HIS C 369
REMARK 620
REMARK 620 METAL COORDINATION
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):
REMARK 620
REMARK 620 COORDINATION ANGLES FOR: M RES CSSEQI METAL
REMARK 620 ALF C1363 AL
REMARK 620 N RES CSSEQI ATOM
REMARK 620 1 ADP C1361 O3B
REMARK 620 2 ALF C1363 F1 96.0
REMARK 620 3 ALF C1363 F2 96.7 166.7
REMARK 620 4 ALF C1363 F3 99.1 87.8 86.6
REMARK 620 5 ALF C1363 F4 97.5 91.5 90.5 163.4
REMARK 620 N 1 2 3 4
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN A 501
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG A 502
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GTP A 1413
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GDP B 1427
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE TA1 B 1428
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ADP C 1361
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG C 1362
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ALF C 1363
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4UXO RELATED DB: PDB
REMARK 900 CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE , ATP
REMARK 900 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
REMARK 900 RELATED ID: 4UXP RELATED DB: PDB
REMARK 900 CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE , ATP
REMARK 900 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
REMARK 900 RELATED ID: 4UXS RELATED DB: PDB
REMARK 900 CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE , ATP
REMARK 900 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
REMARK 900 RELATED ID: 4UXT RELATED DB: PDB
REMARK 900 CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE , ATP
REMARK 900 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
REMARK 900 RELATED ID: 4UXY RELATED DB: PDB
REMARK 900 CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE , ATP
REMARK 900 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
REMARK 900 RELATED ID: 4UY0 RELATED DB: PDB
REMARK 900 CONSERVED MECHANISMS OF MICROTUBULE-STIMULATED ADP RELEASE , ATP
REMARK 900 BINDING, AND FORCE GENERATION IN TRANSPORT KINESINS
REMARK 900 RELATED ID: EMD-2767 RELATED DB: EMDB
DBREF 4UXR A 0 424 UNP P81947 TBA1B_BOVIN 1 451
DBREF 4UXR B 0 444 UNP Q6B856 TBB2B_BOVIN 1 445
DBREF 4UXR C -5 361 UNP Q12756 KIF1A_HUMAN 1 361
SEQADV 4UXR SER A 109 UNP P81947 LEU 136 CONFLICT
SEQADV 4UXR GLY A 205 UNP P81947 GLY 232 CONFLICT
SEQADV 4UXR GLY A 238 UNP P81947 ILE 265 CONFLICT
SEQADV 4UXR THR A 313 UNP P81947 SER 340 CONFLICT
SEQADV 4UXR GLU A 331 UNP P81947 GLN 358 CONFLICT
SEQADV 4UXR VAL B 169 UNP Q6B856 MET 169 CONFLICT
SEQADV 4UXR VAL B 315 UNP Q6B856 ILE 315 CONFLICT
SEQADV 4UXR SER C -5 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR ASP C -4 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR ASN C -3 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR ALA C -2 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR ILE C -1 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR ALA C 0 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 362 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 363 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 364 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 365 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 366 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 367 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 368 UNP Q12756 EXPRESSION TAG
SEQADV 4UXR HIS C 369 UNP Q12756 EXPRESSION TAG
SEQRES 1 A 451 MET ARG GLU CYS ILE SER ILE HIS VAL GLY GLN ALA GLY
SEQRES 2 A 451 VAL GLN ILE GLY ASN ALA CYS TRP GLU LEU TYR CYS LEU
SEQRES 3 A 451 GLU HIS GLY ILE GLN PRO ASP GLY GLN MET PRO SER ASP
SEQRES 4 A 451 LYS THR ILE GLY GLY GLY ASP ASP SER PHE ASN THR PHE
SEQRES 5 A 451 PHE SER GLU THR GLY ALA GLY LYS HIS VAL PRO ARG ALA
SEQRES 6 A 451 VAL PHE VAL ASP LEU GLU PRO THR VAL ILE ASP GLU VAL
SEQRES 7 A 451 ARG THR GLY THR TYR ARG GLN LEU PHE HIS PRO GLU GLN
SEQRES 8 A 451 LEU ILE THR GLY LYS GLU ASP ALA ALA ASN ASN TYR ALA
SEQRES 9 A 451 ARG GLY HIS TYR THR ILE GLY LYS GLU ILE ILE ASP LEU
SEQRES 10 A 451 VAL LEU ASP ARG ILE ARG LYS LEU ALA ASP GLN CYS THR
SEQRES 11 A 451 GLY LEU GLN GLY PHE SER VAL PHE HIS SER PHE GLY GLY
SEQRES 12 A 451 GLY THR GLY SER GLY PHE THR SER LEU LEU MET GLU ARG
SEQRES 13 A 451 LEU SER VAL ASP TYR GLY LYS LYS SER LYS LEU GLU PHE
SEQRES 14 A 451 SER ILE TYR PRO ALA PRO GLN VAL SER THR ALA VAL VAL
SEQRES 15 A 451 GLU PRO TYR ASN SER ILE LEU THR THR HIS THR THR LEU
SEQRES 16 A 451 GLU HIS SER ASP CYS ALA PHE MET VAL ASP ASN GLU ALA
SEQRES 17 A 451 ILE TYR ASP ILE CYS ARG ARG ASN LEU ASP ILE GLU ARG
SEQRES 18 A 451 PRO THR TYR THR ASN LEU ASN ARG LEU ILE GLY GLN ILE
SEQRES 19 A 451 VAL SER SER ILE THR ALA SER LEU ARG PHE ASP GLY ALA
SEQRES 20 A 451 LEU ASN VAL ASP LEU THR GLU PHE GLN THR ASN LEU VAL
SEQRES 21 A 451 PRO TYR PRO ARG GLY HIS PHE PRO LEU ALA THR TYR ALA
SEQRES 22 A 451 PRO VAL ILE SER ALA GLU LYS ALA TYR HIS GLU GLN LEU
SEQRES 23 A 451 SER VAL ALA GLU ILE THR ASN ALA CYS PHE GLU PRO ALA
SEQRES 24 A 451 ASN GLN MET VAL LYS CYS ASP PRO ARG HIS GLY LYS TYR
SEQRES 25 A 451 MET ALA CYS CYS LEU LEU TYR ARG GLY ASP VAL VAL PRO
SEQRES 26 A 451 LYS ASP VAL ASN ALA ALA ILE ALA THR ILE LYS THR LYS
SEQRES 27 A 451 ARG THR ILE GLN PHE VAL ASP TRP CYS PRO THR GLY PHE
SEQRES 28 A 451 LYS VAL GLY ILE ASN TYR GLU PRO PRO THR VAL VAL PRO
SEQRES 29 A 451 GLY GLY ASP LEU ALA LYS VAL GLN ARG ALA VAL CYS MET
SEQRES 30 A 451 LEU SER ASN THR THR ALA ILE ALA GLU ALA TRP ALA ARG
SEQRES 31 A 451 LEU ASP HIS LYS PHE ASP LEU MET TYR ALA LYS ARG ALA
SEQRES 32 A 451 PHE VAL HIS TRP TYR VAL GLY GLU GLY MET GLU GLU GLY
SEQRES 33 A 451 GLU PHE SER GLU ALA ARG GLU ASP MET ALA ALA LEU GLU
SEQRES 34 A 451 LYS ASP TYR GLU GLU VAL GLY VAL ASP SER VAL GLU GLY
SEQRES 35 A 451 GLU GLY GLU GLU GLU GLY GLU GLU TYR
SEQRES 1 B 445 MET ARG GLU ILE VAL HIS ILE GLN ALA GLY GLN CYS GLY
SEQRES 2 B 445 ASN GLN ILE GLY ALA LYS PHE TRP GLU VAL ILE SER ASP
SEQRES 3 B 445 GLU HIS GLY ILE ASP PRO THR GLY SER TYR HIS GLY ASP
SEQRES 4 B 445 SER ASP LEU GLN LEU GLU ARG ILE ASN VAL TYR TYR ASN
SEQRES 5 B 445 GLU ALA ALA GLY ASN LYS TYR VAL PRO ARG ALA ILE LEU
SEQRES 6 B 445 VAL ASP LEU GLU PRO GLY THR MET ASP SER VAL ARG SER
SEQRES 7 B 445 GLY PRO PHE GLY GLN ILE PHE ARG PRO ASP ASN PHE VAL
SEQRES 8 B 445 PHE GLY GLN SER GLY ALA GLY ASN ASN TRP ALA LYS GLY
SEQRES 9 B 445 HIS TYR THR GLU GLY ALA GLU LEU VAL ASP SER VAL LEU
SEQRES 10 B 445 ASP VAL VAL ARG LYS GLU SER GLU SER CYS ASP CYS LEU
SEQRES 11 B 445 GLN GLY PHE GLN LEU THR HIS SER LEU GLY GLY GLY THR
SEQRES 12 B 445 GLY SER GLY MET GLY THR LEU LEU ILE SER LYS ILE ARG
SEQRES 13 B 445 GLU GLU TYR PRO ASP ARG ILE MET ASN THR PHE SER VAL
SEQRES 14 B 445 VAL PRO SER PRO LYS VAL SER ASP THR VAL VAL GLU PRO
SEQRES 15 B 445 TYR ASN ALA THR LEU SER VAL HIS GLN LEU VAL GLU ASN
SEQRES 16 B 445 THR ASP GLU THR TYR CYS ILE ASP ASN GLU ALA LEU TYR
SEQRES 17 B 445 ASP ILE CYS PHE ARG THR LEU LYS LEU THR THR PRO THR
SEQRES 18 B 445 TYR GLY ASP LEU ASN HIS LEU VAL SER ALA THR MET SER
SEQRES 19 B 445 GLY VAL THR THR CYS LEU ARG PHE PRO GLY GLN LEU ASN
SEQRES 20 B 445 ALA ASP LEU ARG LYS LEU ALA VAL ASN MET VAL PRO PHE
SEQRES 21 B 445 PRO ARG LEU HIS PHE PHE MET PRO GLY PHE ALA PRO LEU
SEQRES 22 B 445 THR SER ARG GLY SER GLN GLN TYR ARG ALA LEU THR VAL
SEQRES 23 B 445 PRO GLU LEU THR GLN GLN MET PHE ASP ALA LYS ASN MET
SEQRES 24 B 445 MET ALA ALA CYS ASP PRO ARG HIS GLY ARG TYR LEU THR
SEQRES 25 B 445 VAL ALA ALA VAL PHE ARG GLY ARG MET SER MET LYS GLU
SEQRES 26 B 445 VAL ASP GLU GLN MET LEU ASN VAL GLN ASN LYS ASN SER
SEQRES 27 B 445 SER TYR PHE VAL GLU TRP ILE PRO ASN ASN VAL LYS THR
SEQRES 28 B 445 ALA VAL CYS ASP ILE PRO PRO ARG GLY LEU LYS MET SER
SEQRES 29 B 445 ALA THR PHE ILE GLY ASN SER THR ALA ILE GLN GLU LEU
SEQRES 30 B 445 PHE LYS ARG ILE SER GLU GLN PHE THR ALA MET PHE ARG
SEQRES 31 B 445 ARG LYS ALA PHE LEU HIS TRP TYR THR GLY GLU GLY MET
SEQRES 32 B 445 ASP GLU MET GLU PHE THR GLU ALA GLU SER ASN MET ASN
SEQRES 33 B 445 ASP LEU VAL SER GLU TYR GLN GLN TYR GLN ASP ALA THR
SEQRES 34 B 445 ALA ASP GLU GLN GLY GLU PHE GLU GLU GLU GLY GLU GLU
SEQRES 35 B 445 ASP GLU ALA
SEQRES 1 C 375 SER ASP ASN ALA ILE ALA MET ALA GLY ALA SER VAL LYS
SEQRES 2 C 375 VAL ALA VAL ARG VAL ARG PRO PHE ASN SER ARG GLU MET
SEQRES 3 C 375 SER ARG ASP SER LYS CYS ILE ILE GLN MET SER GLY SER
SEQRES 4 C 375 THR THR THR ILE VAL ASN PRO LYS GLN PRO LYS GLU THR
SEQRES 5 C 375 PRO LYS SER PHE SER PHE ASP TYR SER TYR TRP SER HIS
SEQRES 6 C 375 THR SER PRO GLU ASP ILE ASN TYR ALA SER GLN LYS GLN
SEQRES 7 C 375 VAL TYR ARG ASP ILE GLY GLU GLU MET LEU GLN HIS ALA
SEQRES 8 C 375 PHE GLU GLY TYR ASN VAL CYS ILE PHE ALA TYR GLY GLN
SEQRES 9 C 375 THR GLY ALA GLY LYS SER TYR THR MET MET GLY LYS GLN
SEQRES 10 C 375 GLU LYS ASP GLN GLN GLY ILE ILE PRO GLN LEU CYS GLU
SEQRES 11 C 375 ASP LEU PHE SER ARG ILE ASN ASP THR THR ASN ASP ASN
SEQRES 12 C 375 MET SER TYR SER VAL GLU VAL SER TYR MET GLU ILE TYR
SEQRES 13 C 375 CYS GLU ARG VAL ARG ASP LEU LEU ASN PRO LYS ASN LYS
SEQRES 14 C 375 GLY ASN LEU ARG VAL ARG GLU HIS PRO LEU LEU GLY PRO
SEQRES 15 C 375 TYR VAL GLU ASP LEU SER LYS LEU ALA VAL THR SER TYR
SEQRES 16 C 375 ASN ASP ILE GLN ASP LEU MET ASP SER GLY ASN LYS ALA
SEQRES 17 C 375 ARG THR VAL ALA ALA THR ASN MET ASN GLU THR SER SER
SEQRES 18 C 375 ARG SER HIS ALA VAL PHE ASN ILE ILE PHE THR GLN LYS
SEQRES 19 C 375 ARG HIS ASP ALA GLU THR ASN ILE THR THR GLU LYS VAL
SEQRES 20 C 375 SER LYS ILE SER LEU VAL ASP LEU ALA GLY SER GLU ARG
SEQRES 21 C 375 ALA ASP SER THR GLY ALA LYS GLY THR ARG LEU LYS GLU
SEQRES 22 C 375 GLY ALA ASN ILE ASN LYS SER LEU THR THR LEU GLY LYS
SEQRES 23 C 375 VAL ILE SER ALA LEU ALA GLU MET ASP SER GLY PRO ASN
SEQRES 24 C 375 LYS ASN LYS LYS LYS LYS LYS THR ASP PHE ILE PRO TYR
SEQRES 25 C 375 ARG ASP SER VAL LEU THR TRP LEU LEU ARG GLU ASN LEU
SEQRES 26 C 375 GLY GLY ASN SER ARG THR ALA MET VAL ALA ALA LEU SER
SEQRES 27 C 375 PRO ALA ASP ILE ASN TYR ASP GLU THR LEU SER THR LEU
SEQRES 28 C 375 ARG TYR ALA ASP ARG ALA LYS GLN ILE ARG CYS ASN ALA
SEQRES 29 C 375 VAL ILE ASN HIS HIS HIS HIS HIS HIS HIS HIS
HET ZN A 501 1
HET MG A 502 1
HET GTP A1413 32
HET GDP B1427 28
HET TA1 B1428 62
HET ADP C1361 27
HET MG C1362 1
HET ALF C1363 5
HETNAM ZN ZINC ION
HETNAM MG MAGNESIUM ION
HETNAM GTP GUANOSINE-5'-TRIPHOSPHATE
HETNAM GDP GUANOSINE-5'-DIPHOSPHATE
HETNAM TA1 TAXOL
HETNAM ADP ADENOSINE-5'-DIPHOSPHATE
HETNAM ALF TETRAFLUOROALUMINATE ION
FORMUL 4 ZN ZN 2+
FORMUL 5 MG 2(MG 2+)
FORMUL 6 GTP C10 H16 N5 O14 P3
FORMUL 7 GDP C10 H15 N5 O11 P2
FORMUL 8 TA1 C47 H51 N O14
FORMUL 9 ADP C10 H15 N5 O10 P2
FORMUL 11 ALF AL F4 1-
LINK MG MG A 502 O1G GTP A1413 1555 1555 2.45
LINK O3B ADP C1361 AL ALF C1363 1555 1555 1.98
LINK MG MG C1362 O2B ADP C1361 1555 1555 2.15
SITE 1 AC1 1 HIS A 256
SITE 1 AC2 1 GTP A1413
SITE 1 AC3 9 GLY A 9 GLN A 10 ALA A 11 ALA A 72
SITE 2 AC3 9 ASN A 74 GLY A 116 GLY A 117 THR A 118
SITE 3 AC3 9 MG A 502
SITE 1 AC4 4 GLY B 9 CYS B 11 THR B 142 GLY B 143
SITE 1 AC5 5 VAL B 22 LEU B 272 ARG B 275 PRO B 357
SITE 2 AC5 5 ARG B 358
SITE 1 AC6 4 GLY C 102 SER C 104 MG C1362 ALF C1363
SITE 1 AC7 2 ADP C1361 ALF C1363
SITE 1 AC8 5 SER C 214 ALA C 250 GLY C 251 ADP C1361
SITE 2 AC8 5 MG C1362
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
(ATOM LINES ARE NOT SHOWN.)
END