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Database: PDB
Entry: 4WO1
LinkDB: 4WO1
Original site: 4WO1 
HEADER    SIGNALING PROTEIN                       15-OCT-14   4WO1              
TITLE     CRYSTAL STRUCTURE OF THE DAP12 TRANSMEMBRANE DOMAIN IN LIPID CUBIC    
TITLE    2 PHASE                                                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TYRO PROTEIN TYROSINE KINASE-BINDING PROTEIN;              
COMPND   3 CHAIN: A, B, C, D;                                                   
COMPND   4 FRAGMENT: UNP RESIDUES 35-67;                                        
COMPND   5 SYNONYM: DNAX-ACTIVATION PROTEIN 12,KILLER-ACTIVATING RECEPTOR-      
COMPND   6 ASSOCIATED PROTEIN,KAR-ASSOCIATED PROTEIN;                           
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TYROBP, DAP12, KARAP;                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    ALPHA-HELIX TRANSMEMBRANE SIGNALING, SIGNALING PROTEIN                
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.J.CALL,M.E.CALL,K.KNOBLICH                                          
REVDAT   4   01-JAN-20 4WO1    1       REMARK                                   
REVDAT   3   27-SEP-17 4WO1    1       SOURCE REMARK                            
REVDAT   2   10-JUN-15 4WO1    1       JRNL                                     
REVDAT   1   03-JUN-15 4WO1    0                                                
JRNL        AUTH   K.KNOBLICH,S.PARK,M.LUTFI,L.VAN 'T HAG,C.E.CONN,             
JRNL        AUTH 2 S.A.SEABROOK,J.NEWMAN,P.E.CZABOTAR,W.IM,M.E.CALL,M.J.CALL    
JRNL        TITL   TRANSMEMBRANE COMPLEXES OF DAP12 CRYSTALLIZED IN LIPID       
JRNL        TITL 2 MEMBRANES PROVIDE INSIGHTS INTO CONTROL OF OLIGOMERIZATION   
JRNL        TITL 3 IN IMMUNORECEPTOR ASSEMBLY.                                  
JRNL        REF    CELL REP                      V.  11  1184 2015              
JRNL        REFN                   ESSN 2211-1247                               
JRNL        PMID   25981043                                                     
JRNL        DOI    10.1016/J.CELREP.2015.04.045                                 
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.14 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.9_1692)                     
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.14                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 33.12                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 95.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 6310                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.238                           
REMARK   3   R VALUE            (WORKING SET) : 0.232                           
REMARK   3   FREE R VALUE                     : 0.296                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 9.760                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 616                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 33.1254 -  3.3962    0.94     1462   156  0.2233 0.2947        
REMARK   3     2  3.3962 -  2.6960    0.99     1475   155  0.2398 0.2929        
REMARK   3     3  2.6960 -  2.3553    0.99     1457   165  0.2331 0.2794        
REMARK   3     4  2.3553 -  2.1400    0.90     1300   140  0.2455 0.3264        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.240            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 34.790           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007           1003                                  
REMARK   3   ANGLE     :  0.982           1343                                  
REMARK   3   CHIRALITY :  0.031            186                                  
REMARK   3   PLANARITY :  0.004            153                                  
REMARK   3   DIHEDRAL  : 17.335            358                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 6                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 10 THROUGH 40 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.4712   4.0017 -24.7847              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1836 T22:  -0.0315                                     
REMARK   3      T33:   0.2245 T12:  -0.0052                                     
REMARK   3      T13:   0.0349 T23:   0.0058                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5505 L22:   1.1521                                     
REMARK   3      L33:   5.6678 L12:   0.1928                                     
REMARK   3      L13:  -1.0049 L23:  -0.7563                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1131 S12:   0.2533 S13:  -0.1968                       
REMARK   3      S21:   0.0722 S22:  -0.0522 S23:   0.1870                       
REMARK   3      S31:   0.3628 S32:  -0.1967 S33:   0.1651                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 9 THROUGH 12 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -14.6280   6.5168  -4.1275              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7160 T22:   0.5310                                     
REMARK   3      T33:   0.0035 T12:  -0.1732                                     
REMARK   3      T13:   0.1321 T23:   0.2152                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.2073 L22:   1.0329                                     
REMARK   3      L33:   0.4367 L12:  -0.0377                                     
REMARK   3      L13:   0.8098 L23:   0.3656                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3654 S12:  -0.9377 S13:  -0.4955                       
REMARK   3      S21:   0.5792 S22:   0.0410 S23:   0.1305                       
REMARK   3      S31:   0.4538 S32:   0.8114 S33:   0.4716                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 13 THROUGH 40 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -17.3928  13.8845 -26.8809              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2570 T22:   0.1345                                     
REMARK   3      T33:   0.2371 T12:   0.0205                                     
REMARK   3      T13:  -0.0960 T23:  -0.0431                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0340 L22:   1.5818                                     
REMARK   3      L33:   5.2986 L12:   0.5615                                     
REMARK   3      L13:  -1.6333 L23:  -2.4118                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1079 S12:   0.1189 S13:  -0.0083                       
REMARK   3      S21:   0.2456 S22:  -0.2525 S23:  -0.2284                       
REMARK   3      S31:  -1.3667 S32:  -0.4998 S33:   0.4255                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 9 THROUGH 14 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -18.8941   2.3421  -4.4645              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4186 T22:   0.2317                                     
REMARK   3      T33:  -0.0866 T12:   0.0698                                     
REMARK   3      T13:  -0.0136 T23:   0.0635                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.7611 L22:   4.0972                                     
REMARK   3      L33:   7.8921 L12:  -1.0299                                     
REMARK   3      L13:  -3.4493 L23:   4.1463                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1641 S12:  -0.7444 S13:   0.2304                       
REMARK   3      S21:   0.5895 S22:   0.1883 S23:  -0.0170                       
REMARK   3      S31:  -0.8843 S32:   0.1443 S33:  -0.3143                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 15 THROUGH 40 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -19.8355   3.6687 -28.3311              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1948 T22:   0.1136                                     
REMARK   3      T33:   0.3497 T12:   0.0013                                     
REMARK   3      T13:  -0.0715 T23:  -0.0555                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0354 L22:   3.0970                                     
REMARK   3      L33:   8.5084 L12:   1.4295                                     
REMARK   3      L13:  -1.6979 L23:  -3.0201                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2248 S12:   0.0344 S13:  -0.0125                       
REMARK   3      S21:  -0.2417 S22:   0.2279 S23:   0.3122                       
REMARK   3      S31:  -0.1374 S32:   0.1298 S33:  -0.3287                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 9 THROUGH 40 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  -5.8901  13.8042 -24.4581              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1710 T22:   0.0995                                     
REMARK   3      T33:   0.2976 T12:   0.0073                                     
REMARK   3      T13:  -0.0502 T23:   0.0400                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.2061 L22:   2.4974                                     
REMARK   3      L33:   8.4781 L12:   0.1760                                     
REMARK   3      L13:   0.5484 L23:  -0.8044                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1255 S12:   0.0620 S13:  -0.1455                       
REMARK   3      S21:  -0.0450 S22:  -0.0927 S23:  -0.2728                       
REMARK   3      S31:   0.2205 S32:   0.3071 S33:   0.0203                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4WO1 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 17-OCT-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000204174.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 24-MAY-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.07                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON             
REMARK 200  BEAMLINE                       : MX2                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9537                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : NULL                               
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 6316                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.140                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 33.120                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.7                               
REMARK 200  DATA REDUNDANCY                : 6.600                              
REMARK 200  R MERGE                    (I) : 0.13740                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 15.4600                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: NULL                                                  
REMARK 200 STARTING MODEL: 4WOL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 41.62                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.11                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M BIS-TRIS PROPANE CHLORIDE PH       
REMARK 280  6.07, 19.7 %W/V PEG 3350, 0.27 M CALCIUM CHLORIDE, LIPIDIC CUBIC    
REMARK 280  PHASE, TEMPERATURE 293K                                             
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       25.39850            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       21.84250            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       25.39850            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       21.84250            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1440 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 4770 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -12.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1340 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 5230 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -18.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, C                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     CYS A     8                                                      
REMARK 465     CYS B     8                                                      
REMARK 465     CYS C     8                                                      
REMARK 465     CYS D     8                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     SER A   9    N    CB   OG                                        
REMARK 470     ARG A  39    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER B   9    OG                                                  
REMARK 470     ARG B  39    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER C   9    OG                                                  
REMARK 470     ARG C  39    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER D   9    N    CB   OG                                        
REMARK 470     ARG D  39    CG   CD   NE   CZ   NH1  NH2                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O23  OLC B   101     O    HOH B   201              2.13            
REMARK 500   O    HOH C   206     O    HOH C   211              2.17            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    THR A  10      -91.58   -113.36                                   
REMARK 500    THR B  10     -118.73     54.80                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 610                                                                      
REMARK 610 MISSING HETEROATOM                                                   
REMARK 610 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 610 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 610 I=INSERTION CODE):                                                   
REMARK 610   M RES C SSEQI                                                      
REMARK 610     OLC A  102                                                       
REMARK 610     OLC D  101                                                       
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              CA A 101  CA                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 ASP A  23   OD1                                                    
REMARK 620 2 ASP A  23   OD2  50.1                                              
REMARK 620 3 ASP B  23   OD2  87.7  93.8                                        
REMARK 620 4 HOH A 203   O    51.3 101.0  76.7                                  
REMARK 620 5 HOH A 205   O    86.2  76.4 170.2 105.1                            
REMARK 620 6 HOH B 204   O   144.9 164.1  92.6  94.7  96.8                      
REMARK 620 7 HOH D 205   O   132.6  84.0  84.2 160.5  94.4  82.2                
REMARK 620 N                    1     2     3     4     5     6                 
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              CA A 101  CA                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 ASP A  23   OD1                                                    
REMARK 620 2 ASP B  23   OD1  99.3                                              
REMARK 620 3 ASP B  23   OD2  82.8  47.6                                        
REMARK 620 4 HOH A 203   O    91.6 122.1  78.3                                  
REMARK 620 5 HOH A 204   O    70.6 158.4 143.7  78.2                            
REMARK 620 6 HOH C 205   O   165.5  69.9  95.8 102.2 116.0                      
REMARK 620 7 HOH C 204   O    72.9  77.7 114.9 157.3  81.0  94.9                
REMARK 620 N                    1     2     3     4     5     6                 
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CA A 101                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC A 102                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC B 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC B 102                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC C 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC D 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC D 102                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 2L34   RELATED DB: PDB                                   
REMARK 900 2L34 WAS SOLVED BY NMR                                               
REMARK 900 RELATED ID: 2L35   RELATED DB: PDB                                   
REMARK 900 2L35 WAS SOLVED BY NMR                                               
REMARK 900 RELATED ID: 4WOL   RELATED DB: PDB                                   
DBREF  4WO1 A    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
DBREF  4WO1 B    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
DBREF  4WO1 C    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
DBREF  4WO1 D    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
SEQADV 4WO1 VAL A   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQADV 4WO1 VAL B   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQADV 4WO1 VAL C   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQADV 4WO1 VAL D   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQRES   1 A   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 A   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 A   33  VAL TYR PHE LEU GLY ARG LEU                                  
SEQRES   1 B   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 B   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 B   33  VAL TYR PHE LEU GLY ARG LEU                                  
SEQRES   1 C   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 C   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 C   33  VAL TYR PHE LEU GLY ARG LEU                                  
SEQRES   1 D   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 D   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 D   33  VAL TYR PHE LEU GLY ARG LEU                                  
HET     CA  A 101       2                                                       
HET    OLC  A 102      10                                                       
HET    OLC  B 101      25                                                       
HET    OLC  B 102      25                                                       
HET    OLC  C 101      25                                                       
HET    OLC  D 101      11                                                       
HET    OLC  D 102      25                                                       
HETNAM      CA CALCIUM ION                                                      
HETNAM     OLC (2R)-2,3-DIHYDROXYPROPYL (9Z)-OCTADEC-9-ENOATE                   
HETSYN     OLC 1-OLEOYL-R-GLYCEROL                                              
FORMUL   5   CA    CA 2+                                                        
FORMUL   6  OLC    6(C21 H40 O4)                                                
FORMUL  12  HOH   *31(H2 O)                                                     
HELIX    1 AA1 SER A   12  LEU A   40  1                                  29    
HELIX    2 AA2 SER B   12  ARG B   39  1                                  28    
HELIX    3 AA3 SER C   12  ARG C   39  1                                  28    
HELIX    4 AA4 SER D   12  LEU D   40  1                                  29    
LINK         OD1 ASP A  23                CA  A CA A 101     1555   1555  2.70  
LINK         OD1 ASP A  23                CA  B CA A 101     1555   1555  2.34  
LINK         OD2 ASP A  23                CA  A CA A 101     1555   1555  2.48  
LINK         OD1 ASP B  23                CA  B CA A 101     1555   1555  2.64  
LINK         OD2 ASP B  23                CA  A CA A 101     1555   1555  2.20  
LINK         OD2 ASP B  23                CA  B CA A 101     1555   1555  2.79  
LINK        CA  A CA A 101                 O  AHOH A 203     1555   1555  2.44  
LINK        CA  A CA A 101                 O   HOH A 205     1555   1555  2.33  
LINK        CA  A CA A 101                 O   HOH B 204     1555   1555  2.98  
LINK        CA  A CA A 101                 O   HOH D 205     1555   1555  2.29  
LINK        CA  B CA A 101                 O  BHOH A 203     1555   1555  2.35  
LINK        CA  B CA A 101                 O   HOH A 204     1555   1555  2.77  
LINK        CA  B CA A 101                 O   HOH C 205     1555   1555  2.38  
LINK        CA  B CA A 101                 O   HOH C 204     1555   1555  2.39  
SITE     1 AC1  9 ASP A  23  HOH A 203  HOH A 204  HOH A 205                    
SITE     2 AC1  9 ASP B  23  HOH B 204  HOH C 204  HOH C 205                    
SITE     3 AC1  9 HOH D 205                                                     
SITE     1 AC2  4 VAL A  34  VAL C  34  TYR C  35  OLC D 101                    
SITE     1 AC3  8 GLY A  14  GLY A  18  GLY A  22  LEU B  29                    
SITE     2 AC3  8 PHE B  36  LEU B  40  HOH B 201  VAL C  21                    
SITE     1 AC4  6 LEU A  29  PHE A  36  GLY B  14  GLY B  18                    
SITE     2 AC4  6 VAL B  21  GLY B  22                                          
SITE     1 AC5  7 ALA B  17  GLY C  18  ILE C  19  GLY C  22                    
SITE     2 AC5  7 LEU D  29  LEU D  32  PHE D  36                               
SITE     1 AC6  5 OLC A 102  VAL B  34  ALA D  31  VAL D  34                    
SITE     2 AC6  5 TYR D  35                                                     
SITE     1 AC7  8 VAL A  20  LEU C  29  LEU C  37  VAL D  15                    
SITE     2 AC7  8 GLY D  18  ILE D  19  GLY D  22  LEU D  26                    
CRYST1   50.797   43.685   50.605  90.00  90.00  90.00 P 21 21 2    16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.019686  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.022891  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.019761        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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