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Database: PDB
Entry: 4WOL
LinkDB: 4WOL
Original site: 4WOL 
HEADER    SIGNALING PROTEIN                       16-OCT-14   4WOL              
TITLE     CRYSTAL STRUCTURE OF THE DAP12 TRANSMEMBRANE DOMAIN IN LIPIDIC CUBIC  
TITLE    2 PHASE                                                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TYRO PROTEIN TYROSINE KINASE-BINDING PROTEIN;              
COMPND   3 CHAIN: A, B, C;                                                      
COMPND   4 FRAGMENT: UNP RESIDUES 35-67;                                        
COMPND   5 SYNONYM: DNAX-ACTIVATION PROTEIN 12,KILLER-ACTIVATING RECEPTOR-      
COMPND   6 ASSOCIATED PROTEIN,KAR-ASSOCIATED PROTEIN;                           
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TYROBP, DAP12, KARAP;                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    ALPHA-HELIX TRANSMEMBRANE SIGNALLING, SIGNALING PROTEIN               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.J.CALL,M.E.CALL,K.KNOBLICH                                          
REVDAT   5   01-JAN-20 4WOL    1       REMARK                                   
REVDAT   4   27-SEP-17 4WOL    1       REMARK                                   
REVDAT   3   23-AUG-17 4WOL    1       SOURCE REMARK                            
REVDAT   2   10-JUN-15 4WOL    1       JRNL                                     
REVDAT   1   03-JUN-15 4WOL    0                                                
JRNL        AUTH   K.KNOBLICH,S.PARK,M.LUTFI,L.VAN 'T HAG,C.E.CONN,             
JRNL        AUTH 2 S.A.SEABROOK,J.NEWMAN,P.E.CZABOTAR,W.IM,M.E.CALL,M.J.CALL    
JRNL        TITL   TRANSMEMBRANE COMPLEXES OF DAP12 CRYSTALLIZED IN LIPID       
JRNL        TITL 2 MEMBRANES PROVIDE INSIGHTS INTO CONTROL OF OLIGOMERIZATION   
JRNL        TITL 3 IN IMMUNORECEPTOR ASSEMBLY.                                  
JRNL        REF    CELL REP                      V.  11  1184 2015              
JRNL        REFN                   ESSN 2211-1247                               
JRNL        PMID   25981043                                                     
JRNL        DOI    10.1016/J.CELREP.2015.04.045                                 
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.77 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.9_1692                                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.77                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 33.67                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.330                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 94.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 7407                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.187                           
REMARK   3   R VALUE            (WORKING SET) : 0.183                           
REMARK   3   FREE R VALUE                     : 0.223                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 9.980                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 739                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 33.6754 -  3.0262    0.99     1485   165  0.1536 0.1947        
REMARK   3     2  3.0262 -  2.4022    1.00     1425   159  0.1693 0.1948        
REMARK   3     3  2.4022 -  2.0986    0.93     1279   139  0.2140 0.2808        
REMARK   3     4  2.0986 -  1.9067    0.85     1176   132  0.2358 0.2809        
REMARK   3     5  1.9067 -  1.7701    0.95     1303   144  0.2700 0.2922        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.180            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 25.060           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007            767                                  
REMARK   3   ANGLE     :  0.900           1020                                  
REMARK   3   CHIRALITY :  0.036            137                                  
REMARK   3   PLANARITY :  0.005            116                                  
REMARK   3   DIHEDRAL  : 15.543            286                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 6                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 8 THROUGH 12 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  18.8012  16.9746  74.1459              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7887 T22:   0.3292                                     
REMARK   3      T33:   0.3409 T12:   0.0881                                     
REMARK   3      T13:   0.0482 T23:   0.1679                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.6750 L22:   0.8727                                     
REMARK   3      L33:   5.9069 L12:   1.7911                                     
REMARK   3      L13:  -3.7754 L23:  -1.8145                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0229 S12:  -0.5975 S13:  -0.4615                       
REMARK   3      S21:   0.6136 S22:   0.0802 S23:   0.3953                       
REMARK   3      S31:   0.3558 S32:  -0.1844 S33:  -0.0206                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 13 THROUGH 40 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  15.7472  15.2826  47.8214              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1041 T22:   0.1180                                     
REMARK   3      T33:   0.1688 T12:  -0.0020                                     
REMARK   3      T13:   0.0118 T23:   0.0025                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.4393 L22:   1.7564                                     
REMARK   3      L33:   2.0622 L12:   0.4689                                     
REMARK   3      L13:   0.7566 L23:   0.2437                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0103 S12:   0.0514 S13:  -0.0146                       
REMARK   3      S21:  -0.0425 S22:   0.1625 S23:   0.0022                       
REMARK   3      S31:  -0.1109 S32:   0.0688 S33:  -0.1313                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 8 THROUGH 12 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):   9.9653  14.7514  73.4573              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8917 T22:   0.5477                                     
REMARK   3      T33:   0.2049 T12:  -0.0299                                     
REMARK   3      T13:   0.1164 T23:   0.0646                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.5094 L22:   1.1861                                     
REMARK   3      L33:   1.3785 L12:  -2.2663                                     
REMARK   3      L13:  -0.5302 L23:   0.0175                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0097 S12:  -0.0486 S13:   0.5139                       
REMARK   3      S21:   0.1868 S22:   0.0849 S23:  -0.0742                       
REMARK   3      S31:  -1.4806 S32:   0.6782 S33:  -0.0981                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 13 THROUGH 40 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   5.2183  13.8699  50.6473              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1094 T22:   0.0924                                     
REMARK   3      T33:   0.1586 T12:  -0.0114                                     
REMARK   3      T13:  -0.0050 T23:  -0.0078                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.6745 L22:   1.4941                                     
REMARK   3      L33:   1.3710 L12:  -0.4683                                     
REMARK   3      L13:  -0.2720 L23:  -0.4562                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0023 S12:   0.0838 S13:  -0.0506                       
REMARK   3      S21:  -0.0039 S22:   0.0227 S23:   0.0806                       
REMARK   3      S31:  -0.0255 S32:   0.0802 S33:  -0.0682                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 11 THROUGH 12 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  12.2678  21.5596  71.0064              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6023 T22:   0.7444                                     
REMARK   3      T33:   0.2599 T12:  -0.0076                                     
REMARK   3      T13:  -0.2603 T23:   0.0009                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.2906 L22:   2.0000                                     
REMARK   3      L33:   2.0001 L12:  -1.7888                                     
REMARK   3      L13:   4.0289 L23:   4.3900                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0392 S12:   0.2268 S13:  -0.0872                       
REMARK   3      S21:   0.0068 S22:   0.1398 S23:  -0.3946                       
REMARK   3      S31:   0.5900 S32:   0.8605 S33:  -0.1797                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 13 THROUGH 40 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   9.5569  22.9906  49.4170              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1446 T22:   0.1320                                     
REMARK   3      T33:   0.1506 T12:   0.0140                                     
REMARK   3      T13:  -0.0048 T23:   0.0180                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7908 L22:   0.9924                                     
REMARK   3      L33:   3.8438 L12:   0.1416                                     
REMARK   3      L13:  -1.7868 L23:   0.6198                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1251 S12:  -0.0540 S13:   0.1857                       
REMARK   3      S21:  -0.0462 S22:   0.1723 S23:  -0.0151                       
REMARK   3      S31:  -0.3615 S32:   0.0516 S33:  -0.2439                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4WOL COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 17-OCT-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000204176.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 20-FEB-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON             
REMARK 200  BEAMLINE                       : MX2                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9537                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : NULL                               
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 7502                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.770                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 33.670                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.5                               
REMARK 200  DATA REDUNDANCY                : 13.00                              
REMARK 200  R MERGE                    (I) : 0.13570                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 22.2600                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: NULL                                                  
REMARK 200 STARTING MODEL: 1AFO                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 34.43                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.88                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 12.4% W/V PEG 3350, 0.149 M POTASSIUM    
REMARK 280  THIOCYANATE, LIPIDIC CUBIC PHASE, TEMPERATURE 293K                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       10.38750            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       50.53500            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       17.85450            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       50.53500            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       10.38750            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       17.85450            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2970 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 6660 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -32.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER B     9                                                      
REMARK 465     THR B    10                                                      
REMARK 465     CYS C     8                                                      
REMARK 465     SER C     9                                                      
REMARK 465     THR C    10                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     CYS B   8    N    CA   C    O    CB                              
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                               K A 101   K                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 ASP A  23   OD1                                                    
REMARK 620 2 ASP A  23   OD2  45.0                                              
REMARK 620 3 THR A  27   OG1  80.3  85.4                                        
REMARK 620 4 ASP B  23   OD1  94.0  65.2 141.5                                  
REMARK 620 5 THR B  27   OG1 144.1  99.3  96.6  66.3                            
REMARK 620 6 ASP C  23   OD2  66.5  94.6 130.6  78.6 131.7                      
REMARK 620 7 THR C  27   OG1 113.1 158.0  92.6 124.1 102.7  70.2                
REMARK 620 N                    1     2     3     4     5     6                 
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue K A 101                   
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC B 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC C 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC C 102                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue OLC C 103                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4WO1   RELATED DB: PDB                                   
REMARK 900 4WO1 IS TETRAMERIC FORM                                              
REMARK 900 RELATED ID: 2L34   RELATED DB: PDB                                   
REMARK 900 2L34 WAS SOLVED BY NMR                                               
REMARK 900 RELATED ID: 2L35   RELATED DB: PDB                                   
REMARK 900 2L35 WAS SOLVED BY NMR                                               
DBREF  4WOL A    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
DBREF  4WOL B    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
DBREF  4WOL C    8    40  UNP    O43914   TYOBP_HUMAN     35     67             
SEQADV 4WOL VAL A   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQADV 4WOL VAL B   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQADV 4WOL VAL C   21  UNP  O43914    MET    48 ENGINEERED MUTATION            
SEQRES   1 A   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 A   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 A   33  VAL TYR PHE LEU GLY ARG LEU                                  
SEQRES   1 B   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 B   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 B   33  VAL TYR PHE LEU GLY ARG LEU                                  
SEQRES   1 C   33  CYS SER THR VAL SER PRO GLY VAL LEU ALA GLY ILE VAL          
SEQRES   2 C   33  VAL GLY ASP LEU VAL LEU THR VAL LEU ILE ALA LEU ALA          
SEQRES   3 C   33  VAL TYR PHE LEU GLY ARG LEU                                  
HET      K  A 101       1                                                       
HET    OLC  B 101      25                                                       
HET    OLC  C 101      25                                                       
HET    OLC  C 102      25                                                       
HET    OLC  C 103      25                                                       
HETNAM       K POTASSIUM ION                                                    
HETNAM     OLC (2R)-2,3-DIHYDROXYPROPYL (9Z)-OCTADEC-9-ENOATE                   
HETSYN     OLC 1-OLEOYL-R-GLYCEROL                                              
FORMUL   4    K    K 1+                                                         
FORMUL   5  OLC    4(C21 H40 O4)                                                
FORMUL   9  HOH   *36(H2 O)                                                     
HELIX    1 AA1 SER A   12  ARG A   39  1                                  28    
HELIX    2 AA2 SER B   12  GLY B   38  1                                  27    
HELIX    3 AA3 SER C   12  ARG C   39  1                                  28    
SSBOND   1 CYS A    8    CYS B    8                          1555   1555  2.03  
LINK         OD1 ASP A  23                 K     K A 101     1555   1555  2.86  
LINK         OD2 ASP A  23                 K     K A 101     1555   1555  2.83  
LINK         OG1 THR A  27                 K     K A 101     1555   1555  2.88  
LINK         OD1 ASP B  23                 K     K A 101     1555   1555  3.02  
LINK         OG1 THR B  27                 K     K A 101     1555   1555  2.75  
LINK         OD2 ASP C  23                 K     K A 101     1555   1555  2.69  
LINK         OG1 THR C  27                 K     K A 101     1555   1555  2.83  
SITE     1 AC1  6 ASP A  23  THR A  27  ASP B  23  THR B  27                    
SITE     2 AC1  6 ASP C  23  THR C  27                                          
SITE     1 AC2 12 GLY A  22  ASP A  23  VAL A  34  GLY A  38                    
SITE     2 AC2 12 PRO B  13  LEU B  16  ALA B  17  ASP B  23                    
SITE     3 AC2 12 LEU B  29  ARG B  39  OLC C 101  HOH C 201                    
SITE     1 AC3 11 VAL A  11  LEU A  16  GLY A  18  LEU A  37                    
SITE     2 AC3 11 ILE B  19  ASP B  23  LEU B  26  OLC B 101                    
SITE     3 AC3 11 ASP C  23  HOH C 201  HOH C 204                               
SITE     1 AC4 10 VAL A  20  VAL A  25  ALA B  33  VAL C  25                    
SITE     2 AC4 10 VAL C  28  LEU C  29  PHE C  36  ARG C  39                    
SITE     3 AC4 10 OLC C 103  HOH C 211                                          
SITE     1 AC5 11 LEU B  37  GLY B  38  HOH B 201  GLY C  14                    
SITE     2 AC5 11 VAL C  15  GLY C  18  GLY C  22  LEU C  26                    
SITE     3 AC5 11 OLC C 102  HOH C 202  HOH C 205                               
CRYST1   20.775   35.709  101.070  90.00  90.00  90.00 P 21 21 21   12          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.048135  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.028004  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.009894        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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