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Database: PDB
Entry: 4XBM
LinkDB: 4XBM
Original site: 4XBM 
HEADER    SIGNALING PROTEIN                       17-DEC-14   4XBM              
TITLE     X-RAY CRYSTAL STRUCTURE OF NOTCH LIGAND DELTA-LIKE 1                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DELTA-LIKE PROTEIN 1;                                      
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: DROSOPHILA DELTA HOMOLOG 1,H-DELTA-1;                       
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: DLL1, UNQ146/PRO172;                                           
SOURCE   6 EXPRESSION_SYSTEM: HOMO SAPIENS;                                     
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 9606;                                       
SOURCE   8 EXPRESSION_SYSTEM_CELL_LINE: 293F                                    
KEYWDS    ECTODOMAIN EGF-LIKE REPEAT LIGAND C2 DOMAIN, SIGNALING PROTEIN        
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    N.J.KERSHAW,A.W.BURGESS,N.L.CHURCH,C.S.LUO,T.E.ADAM                   
REVDAT   4   06-SEP-17 4XBM    1       COMPND SOURCE JRNL   REMARK              
REVDAT   3   27-MAY-15 4XBM    1       KEYWDS REMARK                            
REVDAT   2   20-MAY-15 4XBM    1       JRNL                                     
REVDAT   1   11-MAR-15 4XBM    0                                                
JRNL        AUTH   N.J.KERSHAW,N.L.CHURCH,M.D.GRIFFIN,C.S.LUO,T.E.ADAMS,        
JRNL        AUTH 2 A.W.BURGESS                                                  
JRNL        TITL   NOTCH LIGAND DELTA-LIKE1: X-RAY CRYSTAL STRUCTURE AND        
JRNL        TITL 2 BINDING AFFINITY.                                            
JRNL        REF    BIOCHEM.J.                    V. 468   159 2015              
JRNL        REFN                   ESSN 1470-8728                               
JRNL        PMID   25715738                                                     
JRNL        DOI    10.1042/BJ20150010                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.20 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8.4_1496)                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.20                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 67.30                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.380                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 29260                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.260                           
REMARK   3   R VALUE            (WORKING SET) : 0.258                           
REMARK   3   FREE R VALUE                     : 0.284                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 6.830                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1997                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 67.3000 -  6.8920    0.99     2881   213  0.2615 0.2885        
REMARK   3     2  6.8920 -  5.4712    1.00     2774   202  0.2484 0.2577        
REMARK   3     3  5.4712 -  4.7798    1.00     2736   201  0.2031 0.2272        
REMARK   3     4  4.7798 -  4.3429    1.00     2708   199  0.2085 0.2395        
REMARK   3     5  4.3429 -  4.0316    1.00     2710   198  0.2422 0.2796        
REMARK   3     6  4.0316 -  3.7940    1.00     2725   198  0.2713 0.3004        
REMARK   3     7  3.7940 -  3.6040    1.00     2675   196  0.2890 0.3056        
REMARK   3     8  3.6040 -  3.4471    1.00     2706   199  0.3207 0.3420        
REMARK   3     9  3.4471 -  3.3144    1.00     2673   195  0.3437 0.3954        
REMARK   3    10  3.3144 -  3.2000    1.00     2675   196  0.3667 0.3771        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.550            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 30.470           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.004           5176                                  
REMARK   3   ANGLE     :  0.928           6964                                  
REMARK   3   CHIRALITY :  0.036            699                                  
REMARK   3   PLANARITY :  0.007            929                                  
REMARK   3   DIHEDRAL  : 11.728           1857                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4XBM COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 22-DEC-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000205399.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 30-APR-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : AUSTRALIAN SYNCHROTRON             
REMARK 200  BEAMLINE                       : MX2                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9537                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XSCALE                             
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 29271                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.200                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 67.400                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 7.200                              
REMARK 200  R MERGE                    (I) : 0.43200                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 6.2700                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.20                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.31                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB ENTRY 4CBZ                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 67.39                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.77                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.4M POTASSIUM THIOCYANATE 16% PEG3350   
REMARK 280  0.1M BIS-TRIS PROPANE PH 7.5 5MM CACL2, VAPOR DIFFUSION, HANGING    
REMARK 280  DROP, TEMPERATURE 293K                                              
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       54.07500            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       67.40500            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       59.43500            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       67.40500            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       54.07500            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       59.43500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A    21                                                      
REMARK 465     GLY A    48                                                      
REMARK 465     ALA A    49                                                      
REMARK 465     GLY A    50                                                      
REMARK 465     PRO A    51                                                      
REMARK 465     ASP A    96                                                      
REMARK 465     GLY A    97                                                      
REMARK 465     GLY A    98                                                      
REMARK 465     GLY A    99                                                      
REMARK 465     ALA A   100                                                      
REMARK 465     ASP A   101                                                      
REMARK 465     SER A   102                                                      
REMARK 465     ALA A   103                                                      
REMARK 465     PHE A   104                                                      
REMARK 465     SER A   105                                                      
REMARK 465     ASP A   133                                                      
REMARK 465     GLU A   138                                                      
REMARK 465     ASN A   139                                                      
REMARK 465     CYS A   292                                                      
REMARK 465     THR A   293                                                      
REMARK 465     HIS A   294                                                      
REMARK 465     HIS A   295                                                      
REMARK 465     LYS A   296                                                      
REMARK 465     PRO A   297                                                      
REMARK 465     CYS A   298                                                      
REMARK 465     LYS A   299                                                      
REMARK 465     ASN A   300                                                      
REMARK 465     GLY A   301                                                      
REMARK 465     ALA A   302                                                      
REMARK 465     THR A   303                                                      
REMARK 465     CYS A   304                                                      
REMARK 465     THR A   305                                                      
REMARK 465     ASN A   306                                                      
REMARK 465     THR A   307                                                      
REMARK 465     GLY A   308                                                      
REMARK 465     GLN A   309                                                      
REMARK 465     GLY A   310                                                      
REMARK 465     SER A   311                                                      
REMARK 465     TYR A   312                                                      
REMARK 465     THR A   313                                                      
REMARK 465     CYS A   314                                                      
REMARK 465     SER A   315                                                      
REMARK 465     CYS A   316                                                      
REMARK 465     ARG A   317                                                      
REMARK 465     PRO A   318                                                      
REMARK 465     GLY A   319                                                      
REMARK 465     TYR A   320                                                      
REMARK 465     THR A   321                                                      
REMARK 465     GLY A   322                                                      
REMARK 465     ALA A   323                                                      
REMARK 465     THR A   324                                                      
REMARK 465     CYS A   325                                                      
REMARK 465     GLU A   326                                                      
REMARK 465     LEU A   327                                                      
REMARK 465     GLY A   328                                                      
REMARK 465     ILE A   329                                                      
REMARK 465     ASP A   330                                                      
REMARK 465     GLU A   331                                                      
REMARK 465     CYS A   332                                                      
REMARK 465     ASP A   333                                                      
REMARK 465     PRO A   334                                                      
REMARK 465     SER A   335                                                      
REMARK 465     PRO A   336                                                      
REMARK 465     CYS A   337                                                      
REMARK 465     LYS A   338                                                      
REMARK 465     ASN A   339                                                      
REMARK 465     GLY A   340                                                      
REMARK 465     GLY A   341                                                      
REMARK 465     SER A   342                                                      
REMARK 465     CYS A   343                                                      
REMARK 465     THR A   344                                                      
REMARK 465     ASP A   345                                                      
REMARK 465     LEU A   346                                                      
REMARK 465     GLU A   347                                                      
REMARK 465     ASN A   348                                                      
REMARK 465     SER A   349                                                      
REMARK 465     TYR A   350                                                      
REMARK 465     SER A   351                                                      
REMARK 465     CYS A   352                                                      
REMARK 465     THR A   353                                                      
REMARK 465     CYS A   354                                                      
REMARK 465     PRO A   355                                                      
REMARK 465     PRO A   356                                                      
REMARK 465     GLY A   357                                                      
REMARK 465     PHE A   358                                                      
REMARK 465     TYR A   359                                                      
REMARK 465     GLY A   360                                                      
REMARK 465     LYS A   361                                                      
REMARK 465     ILE A   362                                                      
REMARK 465     CYS A   363                                                      
REMARK 465     GLU A   364                                                      
REMARK 465     LEU A   365                                                      
REMARK 465     SER A   366                                                      
REMARK 465     ALA A   367                                                      
REMARK 465     MET A   368                                                      
REMARK 465     THR A   369                                                      
REMARK 465     CYS A   370                                                      
REMARK 465     ALA A   371                                                      
REMARK 465     ASP A   372                                                      
REMARK 465     GLY A   373                                                      
REMARK 465     PRO A   374                                                      
REMARK 465     CYS A   375                                                      
REMARK 465     PHE A   376                                                      
REMARK 465     ASN A   377                                                      
REMARK 465     GLY A   378                                                      
REMARK 465     GLY A   379                                                      
REMARK 465     ARG A   380                                                      
REMARK 465     CYS A   381                                                      
REMARK 465     SER A   382                                                      
REMARK 465     ASP A   383                                                      
REMARK 465     SER A   384                                                      
REMARK 465     PRO A   385                                                      
REMARK 465     ASP A   386                                                      
REMARK 465     GLY A   387                                                      
REMARK 465     GLY A   388                                                      
REMARK 465     TYR A   389                                                      
REMARK 465     SER A   390                                                      
REMARK 465     CYS A   391                                                      
REMARK 465     ARG A   392                                                      
REMARK 465     CYS A   393                                                      
REMARK 465     PRO A   394                                                      
REMARK 465     VAL A   395                                                      
REMARK 465     GLY A   396                                                      
REMARK 465     TYR A   397                                                      
REMARK 465     SER A   398                                                      
REMARK 465     GLY A   399                                                      
REMARK 465     PHE A   400                                                      
REMARK 465     ASN A   401                                                      
REMARK 465     CYS A   402                                                      
REMARK 465     GLU A   403                                                      
REMARK 465     LYS A   404                                                      
REMARK 465     LYS A   405                                                      
REMARK 465     ILE A   406                                                      
REMARK 465     ASP A   407                                                      
REMARK 465     TYR A   408                                                      
REMARK 465     CYS A   409                                                      
REMARK 465     SER A   410                                                      
REMARK 465     SER A   411                                                      
REMARK 465     SER A   412                                                      
REMARK 465     PRO A   413                                                      
REMARK 465     CYS A   414                                                      
REMARK 465     SER A   415                                                      
REMARK 465     ASN A   416                                                      
REMARK 465     GLY A   417                                                      
REMARK 465     ALA A   418                                                      
REMARK 465     LYS A   419                                                      
REMARK 465     CYS A   420                                                      
REMARK 465     VAL A   421                                                      
REMARK 465     ASP A   422                                                      
REMARK 465     LEU A   423                                                      
REMARK 465     GLY A   424                                                      
REMARK 465     ASP A   425                                                      
REMARK 465     ALA A   426                                                      
REMARK 465     TYR A   427                                                      
REMARK 465     LEU A   428                                                      
REMARK 465     CYS A   429                                                      
REMARK 465     ARG A   430                                                      
REMARK 465     CYS A   431                                                      
REMARK 465     GLN A   432                                                      
REMARK 465     ALA A   433                                                      
REMARK 465     GLY A   434                                                      
REMARK 465     PHE A   435                                                      
REMARK 465     SER A   436                                                      
REMARK 465     GLY A   437                                                      
REMARK 465     ARG A   438                                                      
REMARK 465     HIS A   439                                                      
REMARK 465     CYS A   440                                                      
REMARK 465     ASP A   441                                                      
REMARK 465     ASP A   442                                                      
REMARK 465     ASN A   443                                                      
REMARK 465     VAL A   444                                                      
REMARK 465     ASP A   445                                                      
REMARK 465     ASP A   446                                                      
REMARK 465     CYS A   447                                                      
REMARK 465     ALA A   448                                                      
REMARK 465     SER A   449                                                      
REMARK 465     SER A   450                                                      
REMARK 465     PRO A   451                                                      
REMARK 465     CYS A   452                                                      
REMARK 465     ALA A   453                                                      
REMARK 465     ASN A   454                                                      
REMARK 465     GLY A   455                                                      
REMARK 465     GLY A   456                                                      
REMARK 465     THR A   457                                                      
REMARK 465     CYS A   458                                                      
REMARK 465     ARG A   459                                                      
REMARK 465     ASP A   460                                                      
REMARK 465     GLY A   461                                                      
REMARK 465     VAL A   462                                                      
REMARK 465     ASN A   463                                                      
REMARK 465     ASP A   464                                                      
REMARK 465     PHE A   465                                                      
REMARK 465     SER A   466                                                      
REMARK 465     CYS A   467                                                      
REMARK 465     THR A   468                                                      
REMARK 465     CYS A   469                                                      
REMARK 465     PRO A   470                                                      
REMARK 465     PRO A   471                                                      
REMARK 465     GLY A   472                                                      
REMARK 465     TYR A   473                                                      
REMARK 465     THR A   474                                                      
REMARK 465     GLY A   475                                                      
REMARK 465     ARG A   476                                                      
REMARK 465     ASN A   477                                                      
REMARK 465     CYS A   478                                                      
REMARK 465     SER A   479                                                      
REMARK 465     ALA A   480                                                      
REMARK 465     PRO A   481                                                      
REMARK 465     VAL A   482                                                      
REMARK 465     SER A   483                                                      
REMARK 465     ARG A   484                                                      
REMARK 465     CYS A   485                                                      
REMARK 465     GLU A   486                                                      
REMARK 465     HIS A   487                                                      
REMARK 465     ALA A   488                                                      
REMARK 465     PRO A   489                                                      
REMARK 465     CYS A   490                                                      
REMARK 465     HIS A   491                                                      
REMARK 465     ASN A   492                                                      
REMARK 465     GLY A   493                                                      
REMARK 465     ALA A   494                                                      
REMARK 465     THR A   495                                                      
REMARK 465     CYS A   496                                                      
REMARK 465     HIS A   497                                                      
REMARK 465     GLN A   498                                                      
REMARK 465     ARG A   499                                                      
REMARK 465     GLY A   500                                                      
REMARK 465     HIS A   501                                                      
REMARK 465     GLY A   502                                                      
REMARK 465     TYR A   503                                                      
REMARK 465     VAL A   504                                                      
REMARK 465     CYS A   505                                                      
REMARK 465     GLU A   506                                                      
REMARK 465     CYS A   507                                                      
REMARK 465     ALA A   508                                                      
REMARK 465     ARG A   509                                                      
REMARK 465     SER A   510                                                      
REMARK 465     TYR A   511                                                      
REMARK 465     GLY A   512                                                      
REMARK 465     GLY A   513                                                      
REMARK 465     PRO A   514                                                      
REMARK 465     ASN A   515                                                      
REMARK 465     CYS A   516                                                      
REMARK 465     GLN A   517                                                      
REMARK 465     PHE A   518                                                      
REMARK 465     LEU A   519                                                      
REMARK 465     LEU A   520                                                      
REMARK 465     PRO A   521                                                      
REMARK 465     GLU A   522                                                      
REMARK 465     LEU A   523                                                      
REMARK 465     PRO A   524                                                      
REMARK 465     PRO A   525                                                      
REMARK 465     GLY A   526                                                      
REMARK 465     PRO A   527                                                      
REMARK 465     ALA A   528                                                      
REMARK 465     VAL A   529                                                      
REMARK 465     VAL A   530                                                      
REMARK 465     ASP A   531                                                      
REMARK 465     LEU A   532                                                      
REMARK 465     THR A   533                                                      
REMARK 465     GLU A   534                                                      
REMARK 465     LYS A   535                                                      
REMARK 465     LEU A   536                                                      
REMARK 465     GLU A   537                                                      
REMARK 465     GLY A   538                                                      
REMARK 465     GLN A   539                                                      
REMARK 465     GLY A   540                                                      
REMARK 465     GLY A   541                                                      
REMARK 465     PRO A   542                                                      
REMARK 465     PHE A   543                                                      
REMARK 465     PRO A   544                                                      
REMARK 465     TRP A   545                                                      
REMARK 465     GLU A   546                                                      
REMARK 465     ASN A   547                                                      
REMARK 465     LEU A   548                                                      
REMARK 465     TYR A   549                                                      
REMARK 465     PHE A   550                                                      
REMARK 465     GLN A   551                                                      
REMARK 465     SER B    21                                                      
REMARK 465     GLY B    99                                                      
REMARK 465     ALA B   100                                                      
REMARK 465     ASP B   101                                                      
REMARK 465     SER B   102                                                      
REMARK 465     ALA B   103                                                      
REMARK 465     PHE B   104                                                      
REMARK 465     SER B   105                                                      
REMARK 465     ASP B   134                                                      
REMARK 465     LEU B   135                                                      
REMARK 465     ALA B   136                                                      
REMARK 465     THR B   137                                                      
REMARK 465     GLU B   138                                                      
REMARK 465     ASN B   139                                                      
REMARK 465     GLN B   432                                                      
REMARK 465     ALA B   433                                                      
REMARK 465     GLY B   434                                                      
REMARK 465     PHE B   435                                                      
REMARK 465     SER B   436                                                      
REMARK 465     GLY B   437                                                      
REMARK 465     ARG B   438                                                      
REMARK 465     ASN B   443                                                      
REMARK 465     VAL B   444                                                      
REMARK 465     ASP B   445                                                      
REMARK 465     ASP B   446                                                      
REMARK 465     CYS B   447                                                      
REMARK 465     ALA B   448                                                      
REMARK 465     SER B   449                                                      
REMARK 465     SER B   450                                                      
REMARK 465     PRO B   451                                                      
REMARK 465     CYS B   452                                                      
REMARK 465     ALA B   453                                                      
REMARK 465     ASN B   454                                                      
REMARK 465     GLY B   455                                                      
REMARK 465     GLY B   456                                                      
REMARK 465     THR B   457                                                      
REMARK 465     CYS B   458                                                      
REMARK 465     ARG B   459                                                      
REMARK 465     ASP B   460                                                      
REMARK 465     GLY B   461                                                      
REMARK 465     VAL B   462                                                      
REMARK 465     ASN B   463                                                      
REMARK 465     ASP B   464                                                      
REMARK 465     PHE B   465                                                      
REMARK 465     SER B   466                                                      
REMARK 465     CYS B   467                                                      
REMARK 465     THR B   468                                                      
REMARK 465     CYS B   469                                                      
REMARK 465     PRO B   470                                                      
REMARK 465     PRO B   471                                                      
REMARK 465     GLY B   472                                                      
REMARK 465     TYR B   473                                                      
REMARK 465     THR B   474                                                      
REMARK 465     GLY B   475                                                      
REMARK 465     ARG B   476                                                      
REMARK 465     ASN B   477                                                      
REMARK 465     CYS B   478                                                      
REMARK 465     SER B   479                                                      
REMARK 465     ALA B   480                                                      
REMARK 465     PRO B   481                                                      
REMARK 465     VAL B   482                                                      
REMARK 465     SER B   483                                                      
REMARK 465     ARG B   484                                                      
REMARK 465     CYS B   485                                                      
REMARK 465     GLU B   486                                                      
REMARK 465     HIS B   487                                                      
REMARK 465     ALA B   488                                                      
REMARK 465     PRO B   489                                                      
REMARK 465     CYS B   490                                                      
REMARK 465     HIS B   491                                                      
REMARK 465     ASN B   492                                                      
REMARK 465     GLY B   493                                                      
REMARK 465     ALA B   494                                                      
REMARK 465     THR B   495                                                      
REMARK 465     CYS B   496                                                      
REMARK 465     HIS B   497                                                      
REMARK 465     GLN B   498                                                      
REMARK 465     ARG B   499                                                      
REMARK 465     GLY B   500                                                      
REMARK 465     HIS B   501                                                      
REMARK 465     GLY B   502                                                      
REMARK 465     TYR B   503                                                      
REMARK 465     VAL B   504                                                      
REMARK 465     CYS B   505                                                      
REMARK 465     GLU B   506                                                      
REMARK 465     CYS B   507                                                      
REMARK 465     ALA B   508                                                      
REMARK 465     ARG B   509                                                      
REMARK 465     SER B   510                                                      
REMARK 465     TYR B   511                                                      
REMARK 465     GLY B   512                                                      
REMARK 465     GLY B   513                                                      
REMARK 465     PRO B   514                                                      
REMARK 465     ASN B   515                                                      
REMARK 465     CYS B   516                                                      
REMARK 465     GLN B   517                                                      
REMARK 465     PHE B   518                                                      
REMARK 465     LEU B   519                                                      
REMARK 465     LEU B   520                                                      
REMARK 465     PRO B   521                                                      
REMARK 465     GLU B   522                                                      
REMARK 465     LEU B   523                                                      
REMARK 465     PRO B   524                                                      
REMARK 465     PRO B   525                                                      
REMARK 465     GLY B   526                                                      
REMARK 465     PRO B   527                                                      
REMARK 465     ALA B   528                                                      
REMARK 465     VAL B   529                                                      
REMARK 465     VAL B   530                                                      
REMARK 465     ASP B   531                                                      
REMARK 465     LEU B   532                                                      
REMARK 465     THR B   533                                                      
REMARK 465     GLU B   534                                                      
REMARK 465     LYS B   535                                                      
REMARK 465     LEU B   536                                                      
REMARK 465     GLU B   537                                                      
REMARK 465     GLY B   538                                                      
REMARK 465     GLN B   539                                                      
REMARK 465     GLY B   540                                                      
REMARK 465     GLY B   541                                                      
REMARK 465     PRO B   542                                                      
REMARK 465     PHE B   543                                                      
REMARK 465     PRO B   544                                                      
REMARK 465     TRP B   545                                                      
REMARK 465     GLU B   546                                                      
REMARK 465     ASN B   547                                                      
REMARK 465     LEU B   548                                                      
REMARK 465     TYR B   549                                                      
REMARK 465     PHE B   550                                                      
REMARK 465     GLN B   551                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A  46    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     SER A 131    OG                                                  
REMARK 470     ASP A 134    CG   OD1  OD2                                       
REMARK 470     THR A 137    OG1  CG2                                            
REMARK 470     GLU A 141    CG   CD   OE1  OE2                                  
REMARK 470     ASN A 290    CG   OD1  ND2                                       
REMARK 470     TYR A 291    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ASN B 106    CG   OD1  ND2                                       
REMARK 470     ASP B 133    CG   OD1  OD2                                       
REMARK 470     GLU B 141    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 207    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     HIS B 439    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ASP B 441    CG   OD1  OD2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    GLU A   141     CB   LEU A   143              1.89            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    PRO B 385   C   -  N   -  CD  ANGL. DEV. = -17.1 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    GLU A  31      126.23      5.51                                   
REMARK 500    VAL A  33       79.62     38.80                                   
REMARK 500    ASN A  34       99.06    -68.11                                   
REMARK 500    CYS A  45       74.25     57.13                                   
REMARK 500    PRO A  75       66.14   -102.47                                   
REMARK 500    PRO A  76       57.74    -97.14                                   
REMARK 500    ALA A  82      138.82   -170.89                                   
REMARK 500    ASP A  90     -115.15     52.75                                   
REMARK 500    LEU A 135     -153.15    -79.93                                   
REMARK 500    ALA A 136       98.23     55.50                                   
REMARK 500    GLU A 141      -27.48   -142.47                                   
REMARK 500    ARG A 142      -77.56     -8.69                                   
REMARK 500    LEU A 143      178.17     53.73                                   
REMARK 500    ILE A 144      -56.95   -150.99                                   
REMARK 500    LEU A 264      -82.06    -90.88                                   
REMARK 500    LEU A 289       72.36   -109.37                                   
REMARK 500    ASN A 290      -63.26   -146.13                                   
REMARK 500    GLU B  31      141.07      8.85                                   
REMARK 500    PHE B  32      -26.49   -140.83                                   
REMARK 500    VAL B  33      102.40     28.69                                   
REMARK 500    ASN B  41       90.47    -63.10                                   
REMARK 500    PRO B  75       60.30   -104.52                                   
REMARK 500    CYS B  77      107.37    -59.97                                   
REMARK 500    ASP B  90     -113.55     55.53                                   
REMARK 500    THR B 129      -96.19   -103.26                                   
REMARK 500    ARG B 142       68.71   -114.29                                   
REMARK 500    LEU B 143      177.45     43.32                                   
REMARK 500    ILE B 144      -52.54   -162.52                                   
REMARK 500    CYS B 243       96.63   -162.37                                   
REMARK 500    LEU B 264      -82.01    -92.35                                   
REMARK 500    HIS B 294      -63.75    -96.18                                   
REMARK 500    CYS B 325       78.94     54.24                                   
REMARK 500    GLU B 326      -72.72    -97.99                                   
REMARK 500    LEU B 327     -165.85   -118.71                                   
REMARK 500    ILE B 329     -143.24    -78.52                                   
REMARK 500    GLU B 331        3.70    -69.00                                   
REMARK 500    ASP B 386      120.67     -1.66                                   
REMARK 500    ASN B 416     -161.31   -117.71                                   
REMARK 500    ASP B 425        5.96    145.84                                   
REMARK 500    CYS B 440       97.33     51.75                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 ARG A  142     LEU A  143                  142.45                    
REMARK 500 ARG B  142     LEU B  143                  141.93                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Mono-Saccharide FUC B 1500        
REMARK 800  bound to THR B 303                                                  
DBREF  4XBM A   21   545  UNP    O00548   DLL1_HUMAN      21    545             
DBREF  4XBM B   21   545  UNP    O00548   DLL1_HUMAN      21    545             
SEQADV 4XBM GLN A  498  UNP  O00548    GLU   498 CONFLICT                       
SEQADV 4XBM GLY A  502  UNP  O00548    ARG   502 CONFLICT                       
SEQADV 4XBM SER A  510  UNP  O00548    GLY   510 CONFLICT                       
SEQADV 4XBM GLU A  546  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM ASN A  547  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM LEU A  548  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM TYR A  549  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM PHE A  550  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM GLN A  551  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM GLN B  498  UNP  O00548    GLU   498 CONFLICT                       
SEQADV 4XBM GLY B  502  UNP  O00548    ARG   502 CONFLICT                       
SEQADV 4XBM SER B  510  UNP  O00548    GLY   510 CONFLICT                       
SEQADV 4XBM GLU B  546  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM ASN B  547  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM LEU B  548  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM TYR B  549  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM PHE B  550  UNP  O00548              EXPRESSION TAG                 
SEQADV 4XBM GLN B  551  UNP  O00548              EXPRESSION TAG                 
SEQRES   1 A  531  SER SER GLY VAL PHE GLU LEU LYS LEU GLN GLU PHE VAL          
SEQRES   2 A  531  ASN LYS LYS GLY LEU LEU GLY ASN ARG ASN CYS CYS ARG          
SEQRES   3 A  531  GLY GLY ALA GLY PRO PRO PRO CYS ALA CYS ARG THR PHE          
SEQRES   4 A  531  PHE ARG VAL CYS LEU LYS HIS TYR GLN ALA SER VAL SER          
SEQRES   5 A  531  PRO GLU PRO PRO CYS THR TYR GLY SER ALA VAL THR PRO          
SEQRES   6 A  531  VAL LEU GLY VAL ASP SER PHE SER LEU PRO ASP GLY GLY          
SEQRES   7 A  531  GLY ALA ASP SER ALA PHE SER ASN PRO ILE ARG PHE PRO          
SEQRES   8 A  531  PHE GLY PHE THR TRP PRO GLY THR PHE SER LEU ILE ILE          
SEQRES   9 A  531  GLU ALA LEU HIS THR ASP SER PRO ASP ASP LEU ALA THR          
SEQRES  10 A  531  GLU ASN PRO GLU ARG LEU ILE SER ARG LEU ALA THR GLN          
SEQRES  11 A  531  ARG HIS LEU THR VAL GLY GLU GLU TRP SER GLN ASP LEU          
SEQRES  12 A  531  HIS SER SER GLY ARG THR ASP LEU LYS TYR SER TYR ARG          
SEQRES  13 A  531  PHE VAL CYS ASP GLU HIS TYR TYR GLY GLU GLY CYS SER          
SEQRES  14 A  531  VAL PHE CYS ARG PRO ARG ASP ASP ALA PHE GLY HIS PHE          
SEQRES  15 A  531  THR CYS GLY GLU ARG GLY GLU LYS VAL CYS ASN PRO GLY          
SEQRES  16 A  531  TRP LYS GLY PRO TYR CYS THR GLU PRO ILE CYS LEU PRO          
SEQRES  17 A  531  GLY CYS ASP GLU GLN HIS GLY PHE CYS ASP LYS PRO GLY          
SEQRES  18 A  531  GLU CYS LYS CYS ARG VAL GLY TRP GLN GLY ARG TYR CYS          
SEQRES  19 A  531  ASP GLU CYS ILE ARG TYR PRO GLY CYS LEU HIS GLY THR          
SEQRES  20 A  531  CYS GLN GLN PRO TRP GLN CYS ASN CYS GLN GLU GLY TRP          
SEQRES  21 A  531  GLY GLY LEU PHE CYS ASN GLN ASP LEU ASN TYR CYS THR          
SEQRES  22 A  531  HIS HIS LYS PRO CYS LYS ASN GLY ALA THR CYS THR ASN          
SEQRES  23 A  531  THR GLY GLN GLY SER TYR THR CYS SER CYS ARG PRO GLY          
SEQRES  24 A  531  TYR THR GLY ALA THR CYS GLU LEU GLY ILE ASP GLU CYS          
SEQRES  25 A  531  ASP PRO SER PRO CYS LYS ASN GLY GLY SER CYS THR ASP          
SEQRES  26 A  531  LEU GLU ASN SER TYR SER CYS THR CYS PRO PRO GLY PHE          
SEQRES  27 A  531  TYR GLY LYS ILE CYS GLU LEU SER ALA MET THR CYS ALA          
SEQRES  28 A  531  ASP GLY PRO CYS PHE ASN GLY GLY ARG CYS SER ASP SER          
SEQRES  29 A  531  PRO ASP GLY GLY TYR SER CYS ARG CYS PRO VAL GLY TYR          
SEQRES  30 A  531  SER GLY PHE ASN CYS GLU LYS LYS ILE ASP TYR CYS SER          
SEQRES  31 A  531  SER SER PRO CYS SER ASN GLY ALA LYS CYS VAL ASP LEU          
SEQRES  32 A  531  GLY ASP ALA TYR LEU CYS ARG CYS GLN ALA GLY PHE SER          
SEQRES  33 A  531  GLY ARG HIS CYS ASP ASP ASN VAL ASP ASP CYS ALA SER          
SEQRES  34 A  531  SER PRO CYS ALA ASN GLY GLY THR CYS ARG ASP GLY VAL          
SEQRES  35 A  531  ASN ASP PHE SER CYS THR CYS PRO PRO GLY TYR THR GLY          
SEQRES  36 A  531  ARG ASN CYS SER ALA PRO VAL SER ARG CYS GLU HIS ALA          
SEQRES  37 A  531  PRO CYS HIS ASN GLY ALA THR CYS HIS GLN ARG GLY HIS          
SEQRES  38 A  531  GLY TYR VAL CYS GLU CYS ALA ARG SER TYR GLY GLY PRO          
SEQRES  39 A  531  ASN CYS GLN PHE LEU LEU PRO GLU LEU PRO PRO GLY PRO          
SEQRES  40 A  531  ALA VAL VAL ASP LEU THR GLU LYS LEU GLU GLY GLN GLY          
SEQRES  41 A  531  GLY PRO PHE PRO TRP GLU ASN LEU TYR PHE GLN                  
SEQRES   1 B  531  SER SER GLY VAL PHE GLU LEU LYS LEU GLN GLU PHE VAL          
SEQRES   2 B  531  ASN LYS LYS GLY LEU LEU GLY ASN ARG ASN CYS CYS ARG          
SEQRES   3 B  531  GLY GLY ALA GLY PRO PRO PRO CYS ALA CYS ARG THR PHE          
SEQRES   4 B  531  PHE ARG VAL CYS LEU LYS HIS TYR GLN ALA SER VAL SER          
SEQRES   5 B  531  PRO GLU PRO PRO CYS THR TYR GLY SER ALA VAL THR PRO          
SEQRES   6 B  531  VAL LEU GLY VAL ASP SER PHE SER LEU PRO ASP GLY GLY          
SEQRES   7 B  531  GLY ALA ASP SER ALA PHE SER ASN PRO ILE ARG PHE PRO          
SEQRES   8 B  531  PHE GLY PHE THR TRP PRO GLY THR PHE SER LEU ILE ILE          
SEQRES   9 B  531  GLU ALA LEU HIS THR ASP SER PRO ASP ASP LEU ALA THR          
SEQRES  10 B  531  GLU ASN PRO GLU ARG LEU ILE SER ARG LEU ALA THR GLN          
SEQRES  11 B  531  ARG HIS LEU THR VAL GLY GLU GLU TRP SER GLN ASP LEU          
SEQRES  12 B  531  HIS SER SER GLY ARG THR ASP LEU LYS TYR SER TYR ARG          
SEQRES  13 B  531  PHE VAL CYS ASP GLU HIS TYR TYR GLY GLU GLY CYS SER          
SEQRES  14 B  531  VAL PHE CYS ARG PRO ARG ASP ASP ALA PHE GLY HIS PHE          
SEQRES  15 B  531  THR CYS GLY GLU ARG GLY GLU LYS VAL CYS ASN PRO GLY          
SEQRES  16 B  531  TRP LYS GLY PRO TYR CYS THR GLU PRO ILE CYS LEU PRO          
SEQRES  17 B  531  GLY CYS ASP GLU GLN HIS GLY PHE CYS ASP LYS PRO GLY          
SEQRES  18 B  531  GLU CYS LYS CYS ARG VAL GLY TRP GLN GLY ARG TYR CYS          
SEQRES  19 B  531  ASP GLU CYS ILE ARG TYR PRO GLY CYS LEU HIS GLY THR          
SEQRES  20 B  531  CYS GLN GLN PRO TRP GLN CYS ASN CYS GLN GLU GLY TRP          
SEQRES  21 B  531  GLY GLY LEU PHE CYS ASN GLN ASP LEU ASN TYR CYS THR          
SEQRES  22 B  531  HIS HIS LYS PRO CYS LYS ASN GLY ALA THR CYS THR ASN          
SEQRES  23 B  531  THR GLY GLN GLY SER TYR THR CYS SER CYS ARG PRO GLY          
SEQRES  24 B  531  TYR THR GLY ALA THR CYS GLU LEU GLY ILE ASP GLU CYS          
SEQRES  25 B  531  ASP PRO SER PRO CYS LYS ASN GLY GLY SER CYS THR ASP          
SEQRES  26 B  531  LEU GLU ASN SER TYR SER CYS THR CYS PRO PRO GLY PHE          
SEQRES  27 B  531  TYR GLY LYS ILE CYS GLU LEU SER ALA MET THR CYS ALA          
SEQRES  28 B  531  ASP GLY PRO CYS PHE ASN GLY GLY ARG CYS SER ASP SER          
SEQRES  29 B  531  PRO ASP GLY GLY TYR SER CYS ARG CYS PRO VAL GLY TYR          
SEQRES  30 B  531  SER GLY PHE ASN CYS GLU LYS LYS ILE ASP TYR CYS SER          
SEQRES  31 B  531  SER SER PRO CYS SER ASN GLY ALA LYS CYS VAL ASP LEU          
SEQRES  32 B  531  GLY ASP ALA TYR LEU CYS ARG CYS GLN ALA GLY PHE SER          
SEQRES  33 B  531  GLY ARG HIS CYS ASP ASP ASN VAL ASP ASP CYS ALA SER          
SEQRES  34 B  531  SER PRO CYS ALA ASN GLY GLY THR CYS ARG ASP GLY VAL          
SEQRES  35 B  531  ASN ASP PHE SER CYS THR CYS PRO PRO GLY TYR THR GLY          
SEQRES  36 B  531  ARG ASN CYS SER ALA PRO VAL SER ARG CYS GLU HIS ALA          
SEQRES  37 B  531  PRO CYS HIS ASN GLY ALA THR CYS HIS GLN ARG GLY HIS          
SEQRES  38 B  531  GLY TYR VAL CYS GLU CYS ALA ARG SER TYR GLY GLY PRO          
SEQRES  39 B  531  ASN CYS GLN PHE LEU LEU PRO GLU LEU PRO PRO GLY PRO          
SEQRES  40 B  531  ALA VAL VAL ASP LEU THR GLU LYS LEU GLU GLY GLN GLY          
SEQRES  41 B  531  GLY PRO PHE PRO TRP GLU ASN LEU TYR PHE GLN                  
HET    FUC  B1500      10                                                       
HETNAM     FUC ALPHA-L-FUCOSE                                                   
FORMUL   3  FUC    C6 H12 O5                                                    
HELIX    1 AA1 CYS B   45  ALA B   49  5                                   5    
HELIX    2 AA2 TYR B  291  LYS B  296  1                                   6    
HELIX    3 AA3 ASP B  407  SER B  412  5                                   6    
SHEET    1 AA1 4 ILE A 108  PHE A 112  0                                        
SHEET    2 AA1 4 GLY A  23  GLN A  30 -1  N  GLY A  23   O  PHE A 112           
SHEET    3 AA1 4 THR A 169  CYS A 179 -1  O  LYS A 172   N  GLN A  30           
SHEET    4 AA1 4 GLY A 156  SER A 166 -1  N  ASP A 162   O  TYR A 173           
SHEET    1 AA2 3 GLY A  80  VAL A  83  0                                        
SHEET    2 AA2 3 ARG A  57  HIS A  66 -1  N  LEU A  64   O  GLY A  80           
SHEET    3 AA2 3 GLY A  88  VAL A  89 -1  O  GLY A  88   N  THR A  58           
SHEET    1 AA3 4 GLY A  80  VAL A  83  0                                        
SHEET    2 AA3 4 ARG A  57  HIS A  66 -1  N  LEU A  64   O  GLY A  80           
SHEET    3 AA3 4 THR A 119  HIS A 128 -1  O  LEU A 127   N  PHE A  59           
SHEET    4 AA3 4 ILE A 144  HIS A 152 -1  O  ARG A 151   N  PHE A 120           
SHEET    1 AA4 2 TYR A 183  TYR A 184  0                                        
SHEET    2 AA4 2 VAL A 190  PHE A 191 -1  O  VAL A 190   N  TYR A 184           
SHEET    1 AA5 3 ARG A 195  ASP A 197  0                                        
SHEET    2 AA5 3 GLY A 200  CYS A 204 -1  O  GLY A 200   N  ASP A 197           
SHEET    3 AA5 3 LYS A 210  CYS A 212 -1  O  VAL A 211   N  THR A 203           
SHEET    1 AA6 2 TRP A 216  LYS A 217  0                                        
SHEET    2 AA6 2 GLU A 223  PRO A 224 -1  O  GLU A 223   N  LYS A 217           
SHEET    1 AA7 2 GLY A 235  PHE A 236  0                                        
SHEET    2 AA7 2 LYS A 244  CYS A 245 -1  O  LYS A 244   N  PHE A 236           
SHEET    1 AA8 2 TRP A 249  GLN A 250  0                                        
SHEET    2 AA8 2 GLU A 256  CYS A 257 -1  O  GLU A 256   N  GLN A 250           
SHEET    1 AA9 2 GLY A 266  THR A 267  0                                        
SHEET    2 AA9 2 ASN A 275  CYS A 276 -1  O  ASN A 275   N  THR A 267           
SHEET    1 AB1 2 TRP A 280  GLY A 281  0                                        
SHEET    2 AB1 2 GLN A 287  ASP A 288 -1  O  GLN A 287   N  GLY A 281           
SHEET    1 AB2 4 ILE B 108  PHE B 112  0                                        
SHEET    2 AB2 4 GLY B  23  GLN B  30 -1  N  GLY B  23   O  PHE B 112           
SHEET    3 AB2 4 ASP B 170  CYS B 179 -1  O  VAL B 178   N  VAL B  24           
SHEET    4 AB2 4 GLY B 156  SER B 165 -1  N  ASP B 162   O  TYR B 173           
SHEET    1 AB3 4 GLY B  80  VAL B  89  0                                        
SHEET    2 AB3 4 ARG B  57  LYS B  65 -1  N  LEU B  64   O  GLY B  80           
SHEET    3 AB3 4 THR B 119  HIS B 128 -1  O  LEU B 127   N  PHE B  59           
SHEET    4 AB3 4 ILE B 144  HIS B 152 -1  O  ARG B 151   N  PHE B 120           
SHEET    1 AB4 2 TYR B 183  TYR B 184  0                                        
SHEET    2 AB4 2 VAL B 190  PHE B 191 -1  O  VAL B 190   N  TYR B 184           
SHEET    1 AB5 3 ARG B 195  ASP B 197  0                                        
SHEET    2 AB5 3 GLY B 200  CYS B 204 -1  O  GLY B 200   N  ASP B 197           
SHEET    3 AB5 3 LYS B 210  CYS B 212 -1  O  VAL B 211   N  THR B 203           
SHEET    1 AB6 2 TRP B 216  LYS B 217  0                                        
SHEET    2 AB6 2 GLU B 223  PRO B 224 -1  O  GLU B 223   N  LYS B 217           
SHEET    1 AB7 2 GLY B 235  PHE B 236  0                                        
SHEET    2 AB7 2 LYS B 244  CYS B 245 -1  O  LYS B 244   N  PHE B 236           
SHEET    1 AB8 2 TRP B 249  GLN B 250  0                                        
SHEET    2 AB8 2 GLU B 256  CYS B 257 -1  O  GLU B 256   N  GLN B 250           
SHEET    1 AB9 2 GLY B 266  THR B 267  0                                        
SHEET    2 AB9 2 ASN B 275  CYS B 276 -1  O  ASN B 275   N  THR B 267           
SHEET    1 AC1 2 TRP B 280  GLY B 281  0                                        
SHEET    2 AC1 2 GLN B 287  ASP B 288 -1  O  GLN B 287   N  GLY B 281           
SHEET    1 AC2 2 THR B 303  ASN B 306  0                                        
SHEET    2 AC2 2 TYR B 312  SER B 315 -1  O  SER B 315   N  THR B 303           
SHEET    1 AC3 2 SER B 342  LEU B 346  0                                        
SHEET    2 AC3 2 SER B 349  THR B 353 -1  O  SER B 351   N  THR B 344           
SHEET    1 AC4 2 PHE B 358  TYR B 359  0                                        
SHEET    2 AC4 2 LEU B 365  SER B 366 -1  O  LEU B 365   N  TYR B 359           
SHEET    1 AC5 2 ARG B 380  ASP B 383  0                                        
SHEET    2 AC5 2 TYR B 389  ARG B 392 -1  O  ARG B 392   N  ARG B 380           
SHEET    1 AC6 2 LYS B 419  ASP B 422  0                                        
SHEET    2 AC6 2 TYR B 427  ARG B 430 -1  O  LEU B 428   N  VAL B 421           
SSBOND   1 CYS A   44    CYS A   56                          1555   1555  2.03  
SSBOND   2 CYS A   45    CYS A   54                          1555   1555  2.03  
SSBOND   3 CYS A   63    CYS A   77                          1555   1555  2.03  
SSBOND   4 CYS A  179    CYS A  188                          1555   1555  2.03  
SSBOND   5 CYS A  192    CYS A  204                          1555   1555  2.04  
SSBOND   6 CYS A  212    CYS A  221                          1555   1555  2.03  
SSBOND   7 CYS A  226    CYS A  237                          1555   1555  2.03  
SSBOND   8 CYS A  230    CYS A  243                          1555   1555  2.04  
SSBOND   9 CYS A  245    CYS A  254                          1555   1555  2.03  
SSBOND  10 CYS A  257    CYS A  268                          1555   1555  2.04  
SSBOND  11 CYS A  263    CYS A  274                          1555   1555  2.04  
SSBOND  12 CYS A  276    CYS A  285                          1555   1555  2.03  
SSBOND  13 CYS B   44    CYS B   56                          1555   1555  2.03  
SSBOND  14 CYS B   45    CYS B   54                          1555   1555  2.04  
SSBOND  15 CYS B   63    CYS B   77                          1555   1555  2.04  
SSBOND  16 CYS B  179    CYS B  188                          1555   1555  2.03  
SSBOND  17 CYS B  192    CYS B  204                          1555   1555  2.04  
SSBOND  18 CYS B  212    CYS B  221                          1555   1555  2.04  
SSBOND  19 CYS B  226    CYS B  237                          1555   1555  2.04  
SSBOND  20 CYS B  230    CYS B  243                          1555   1555  2.04  
SSBOND  21 CYS B  245    CYS B  254                          1555   1555  2.04  
SSBOND  22 CYS B  257    CYS B  268                          1555   1555  2.04  
SSBOND  23 CYS B  263    CYS B  274                          1555   1555  2.04  
SSBOND  24 CYS B  276    CYS B  285                          1555   1555  2.03  
SSBOND  25 CYS B  292    CYS B  304                          1555   1555  2.00  
SSBOND  26 CYS B  298    CYS B  314                          1555   1555  2.03  
SSBOND  27 CYS B  316    CYS B  325                          1555   1555  2.03  
SSBOND  28 CYS B  337    CYS B  352                          1555   1555  2.04  
SSBOND  29 CYS B  354    CYS B  363                          1555   1555  2.03  
SSBOND  30 CYS B  370    CYS B  381                          1555   1555  2.04  
SSBOND  31 CYS B  375    CYS B  391                          1555   1555  2.03  
SSBOND  32 CYS B  393    CYS B  402                          1555   1555  2.04  
SSBOND  33 CYS B  409    CYS B  420                          1555   1555  2.04  
SSBOND  34 CYS B  414    CYS B  429                          1555   1555  2.04  
SSBOND  35 CYS B  431    CYS B  440                          1555   1555  2.03  
LINK         OG1 THR B 303                 C1  FUC B1500     1555   1555  1.44  
SITE     1 AC1  2 GLY B 301  THR B 303                                          
CRYST1  108.150  118.870  134.810  90.00  90.00  90.00 P 21 21 21    8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009246  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.008413  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.007418        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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