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Database: PDB
Entry: 5BT1
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HEADER    CHAPERONE                               02-JUN-15   5BT1              
TITLE     HISTONE CHAPERONE HIF1 PLAYING WITH HISTONE H2A-H2B DIMER             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: HAT1-INTERACTING FACTOR 1;                                 
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: HISTONE H2A.1;                                             
COMPND   7 CHAIN: C;                                                            
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 3;                                                           
COMPND  10 MOLECULE: HISTONE H2B.1;                                             
COMPND  11 CHAIN: D;                                                            
COMPND  12 SYNONYM: SUPPRESSOR OF TY PROTEIN 12;                                
COMPND  13 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE (STRAIN ATCC 204508 /  
SOURCE   3 S288C);                                                              
SOURCE   4 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE   5 ORGANISM_TAXID: 559292;                                              
SOURCE   6 STRAIN: ATCC 204508 / S288C;                                         
SOURCE   7 GENE: HIF1, YLL022C, L1205;                                          
SOURCE   8 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   9 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE (STRAIN ATCC 204508 /  
SOURCE  12 S288C);                                                              
SOURCE  13 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  14 ORGANISM_TAXID: 559292;                                              
SOURCE  15 STRAIN: ATCC 204508 / S288C;                                         
SOURCE  16 GENE: HTA1, H2A1, SPT11, YDR225W, YD9934.10;                         
SOURCE  17 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  18 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  19 MOL_ID: 3;                                                           
SOURCE  20 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE (STRAIN ATCC 204508 /  
SOURCE  21 S288C);                                                              
SOURCE  22 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  23 ORGANISM_TAXID: 559292;                                              
SOURCE  24 STRAIN: ATCC 204508 / S288C;                                         
SOURCE  25 GENE: HTB1, H2B1, SPT12, YDR224C, YD9934.09C;                        
SOURCE  26 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  27 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    HISTONE CHAPERONE COMPLEX, TPR, NASP HOMOLOG, ASSEMBLY, CHAPERONE     
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    H.LIU,M.ZHANG,Y.GAO,M.TENG,L.NIU                                      
REVDAT   3   18-OCT-17 5BT1    1       REMARK                                   
REVDAT   2   27-SEP-17 5BT1    1       JRNL   REMARK                            
REVDAT   1   26-OCT-16 5BT1    0                                                
JRNL        AUTH   M.ZHANG,H.LIU,Y.GAO,Z.ZHU,Z.CHEN,P.ZHENG,L.XUE,J.LI,M.TENG,  
JRNL        AUTH 2 L.NIU                                                        
JRNL        TITL   STRUCTURAL INSIGHTS INTO THE ASSOCIATION OF HIF1 WITH        
JRNL        TITL 2 HISTONES H2A-H2B DIMER AND H3-H4 TETRAMER                    
JRNL        REF    STRUCTURE                     V.  24  1810 2016              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   27618665                                                     
JRNL        DOI    10.1016/J.STR.2016.08.001                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.62 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.5.0109                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.62                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.38                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.1                           
REMARK   3   NUMBER OF REFLECTIONS             : 21504                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.210                           
REMARK   3   R VALUE            (WORKING SET) : 0.208                           
REMARK   3   FREE R VALUE                     : 0.262                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.100                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1162                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.62                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.69                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 1591                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 97.04                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2820                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 79                           
REMARK   3   BIN FREE R VALUE                    : 0.3700                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 5270                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 47                                      
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 45.25                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 0.49000                                              
REMARK   3    B22 (A**2) : -0.32000                                             
REMARK   3    B33 (A**2) : 0.80000                                              
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 1.66000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 2.644         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.349         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.264         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 27.950        
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.936                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.898                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  5350 ; 0.008 ; 0.022       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  7222 ; 1.049 ; 1.970       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   662 ; 4.681 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   262 ;41.213 ;24.771       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   974 ;19.088 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    37 ;21.287 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   821 ; 0.069 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  4017 ; 0.004 ; 0.021       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  3330 ; 0.533 ; 1.500       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  5323 ; 1.007 ; 2.000       
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  2020 ; 1.225 ; 3.000       
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1899 ; 2.174 ; 4.500       
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A    15        A   346                          
REMARK   3    ORIGIN FOR THE GROUP (A): -12.6734  -4.1717 -63.4318              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1208 T22:   0.1012                                     
REMARK   3      T33:   0.1156 T12:   0.0035                                     
REMARK   3      T13:   0.0281 T23:   0.0018                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4381 L22:   1.8289                                     
REMARK   3      L33:   0.4054 L12:   0.8305                                     
REMARK   3      L13:   0.1576 L23:   0.4070                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0596 S12:  -0.0770 S13:  -0.0051                       
REMARK   3      S21:  -0.0494 S22:  -0.1173 S23:  -0.0400                       
REMARK   3      S31:   0.0105 S32:   0.0248 S33:   0.0577                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B    17        B   345                          
REMARK   3    ORIGIN FOR THE GROUP (A): -42.6583  -1.5173 -19.1718              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1218 T22:   0.0956                                     
REMARK   3      T33:   0.1259 T12:  -0.0384                                     
REMARK   3      T13:   0.0098 T23:  -0.0109                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3497 L22:   1.7246                                     
REMARK   3      L33:   0.7952 L12:   0.0980                                     
REMARK   3      L13:  -0.1198 L23:  -1.1339                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0109 S12:  -0.0066 S13:   0.0138                       
REMARK   3      S21:   0.0464 S22:  -0.0116 S23:   0.0688                       
REMARK   3      S31:  -0.0201 S32:   0.0294 S33:   0.0225                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   C    16        C   100                          
REMARK   3    ORIGIN FOR THE GROUP (A): -16.7169   5.8583 -35.5902              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1171 T22:   0.1472                                     
REMARK   3      T33:   0.1116 T12:  -0.0541                                     
REMARK   3      T13:  -0.0352 T23:   0.1030                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.0705 L22:   3.0256                                     
REMARK   3      L33:   1.3583 L12:  -0.2615                                     
REMARK   3      L13:  -0.7160 L23:  -1.5927                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1240 S12:  -0.1231 S13:  -0.2801                       
REMARK   3      S21:   0.0431 S22:   0.0262 S23:   0.1666                       
REMARK   3      S31:  -0.1058 S32:   0.1336 S33:   0.0978                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   D    37        D   126                          
REMARK   3    ORIGIN FOR THE GROUP (A): -15.0209   9.8439 -38.1260              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0498 T22:   0.1277                                     
REMARK   3      T33:   0.1504 T12:  -0.0455                                     
REMARK   3      T13:  -0.0022 T23:   0.0834                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7865 L22:   2.8296                                     
REMARK   3      L33:   2.0138 L12:   0.2751                                     
REMARK   3      L13:  -0.2411 L23:  -2.2894                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1616 S12:  -0.1248 S13:  -0.1596                       
REMARK   3      S21:   0.0655 S22:  -0.0801 S23:  -0.1094                       
REMARK   3      S31:  -0.0885 S32:   0.1861 S33:   0.2417                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : MASK                                                 
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.40                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING   
REMARK   3  POSITIONS U VALUES : WITH TLS ADDED                                 
REMARK   4                                                                      
REMARK   4 5BT1 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 03-JUN-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000210453.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 14-FEB-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRF                               
REMARK 200  BEAMLINE                       : BL17U                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97915                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 22738                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.620                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.5                               
REMARK 200  DATA REDUNDANCY                : 3.600                              
REMARK 200  R MERGE                    (I) : 0.10500                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 7.4000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.62                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.67                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.7                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.50                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.62600                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 4NQ0                                                 
REMARK 200                                                                      
REMARK 200 REMARK: ROD-SHAPED CRYSTALS                                          
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 22.61                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.59                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M MAGNESIUM FORMATE DIHYDRATE, 15%    
REMARK 280  PEG3350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K            
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       51.33650            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       24.18150            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       51.33650            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       24.18150            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     LYS A     2                                                      
REMARK 465     LEU A     3                                                      
REMARK 465     ARG A     4                                                      
REMARK 465     ALA A     5                                                      
REMARK 465     GLU A     6                                                      
REMARK 465     ASP A     7                                                      
REMARK 465     VAL A     8                                                      
REMARK 465     LEU A     9                                                      
REMARK 465     ALA A    10                                                      
REMARK 465     ASN A    11                                                      
REMARK 465     GLY A    12                                                      
REMARK 465     THR A    13                                                      
REMARK 465     SER A    14                                                      
REMARK 465     GLY A    80                                                      
REMARK 465     ASN A    81                                                      
REMARK 465     LEU A    82                                                      
REMARK 465     PHE A    83                                                      
REMARK 465     GLY A    84                                                      
REMARK 465     ASP A    85                                                      
REMARK 465     ALA A    86                                                      
REMARK 465     LEU A    87                                                      
REMARK 465     LEU A    88                                                      
REMARK 465     ALA A    89                                                      
REMARK 465     GLY A    90                                                      
REMARK 465     ASP A    91                                                      
REMARK 465     ASP A    92                                                      
REMARK 465     GLY A    93                                                      
REMARK 465     SER A    94                                                      
REMARK 465     GLY A    95                                                      
REMARK 465     SER A    96                                                      
REMARK 465     GLU A    97                                                      
REMARK 465     SER A    98                                                      
REMARK 465     GLU A    99                                                      
REMARK 465     SER A   100                                                      
REMARK 465     GLU A   101                                                      
REMARK 465     PRO A   102                                                      
REMARK 465     GLU A   103                                                      
REMARK 465     SER A   104                                                      
REMARK 465     ASP A   105                                                      
REMARK 465     VAL A   106                                                      
REMARK 465     SER A   107                                                      
REMARK 465     ASN A   108                                                      
REMARK 465     GLY A   109                                                      
REMARK 465     GLU A   110                                                      
REMARK 465     GLU A   111                                                      
REMARK 465     GLY A   112                                                      
REMARK 465     ASN A   113                                                      
REMARK 465     GLU A   114                                                      
REMARK 465     ASN A   115                                                      
REMARK 465     GLY A   116                                                      
REMARK 465     GLN A   117                                                      
REMARK 465     THR A   118                                                      
REMARK 465     GLU A   119                                                      
REMARK 465     ILE A   120                                                      
REMARK 465     PRO A   121                                                      
REMARK 465     ASN A   122                                                      
REMARK 465     SER A   123                                                      
REMARK 465     ARG A   124                                                      
REMARK 465     MET A   125                                                      
REMARK 465     PHE A   126                                                      
REMARK 465     GLN A   127                                                      
REMARK 465     PHE A   128                                                      
REMARK 465     ASP A   129                                                      
REMARK 465     GLN A   130                                                      
REMARK 465     GLU A   131                                                      
REMARK 465     GLU A   132                                                      
REMARK 465     GLU A   133                                                      
REMARK 465     ASP A   134                                                      
REMARK 465     LEU A   135                                                      
REMARK 465     THR A   136                                                      
REMARK 465     GLY A   137                                                      
REMARK 465     ASP A   138                                                      
REMARK 465     VAL A   139                                                      
REMARK 465     ASP A   140                                                      
REMARK 465     SER A   141                                                      
REMARK 465     GLY A   142                                                      
REMARK 465     ASP A   143                                                      
REMARK 465     SER A   144                                                      
REMARK 465     GLU A   145                                                      
REMARK 465     ASP A   146                                                      
REMARK 465     SER A   147                                                      
REMARK 465     GLY A   148                                                      
REMARK 465     GLU A   149                                                      
REMARK 465     GLY A   150                                                      
REMARK 465     SER A   151                                                      
REMARK 465     GLU A   152                                                      
REMARK 465     GLU A   153                                                      
REMARK 465     GLU A   154                                                      
REMARK 465     GLU A   155                                                      
REMARK 465     GLU A   156                                                      
REMARK 465     ASN A   157                                                      
REMARK 465     VAL A   158                                                      
REMARK 465     GLU A   159                                                      
REMARK 465     LYS A   160                                                      
REMARK 465     GLU A   161                                                      
REMARK 465     GLU A   162                                                      
REMARK 465     GLU A   163                                                      
REMARK 465     ARG A   164                                                      
REMARK 465     LEU A   165                                                      
REMARK 465     ALA A   166                                                      
REMARK 465     LEU A   167                                                      
REMARK 465     GLY A   347                                                      
REMARK 465     SER A   348                                                      
REMARK 465     LYS A   349                                                      
REMARK 465     ARG A   350                                                      
REMARK 465     PRO A   351                                                      
REMARK 465     LEU A   352                                                      
REMARK 465     SER A   353                                                      
REMARK 465     GLN A   354                                                      
REMARK 465     PRO A   355                                                      
REMARK 465     THR A   356                                                      
REMARK 465     THR A   357                                                      
REMARK 465     SER A   358                                                      
REMARK 465     ILE A   359                                                      
REMARK 465     GLY A   360                                                      
REMARK 465     PHE A   361                                                      
REMARK 465     PRO A   362                                                      
REMARK 465     ALA A   363                                                      
REMARK 465     LEU A   364                                                      
REMARK 465     GLU A   365                                                      
REMARK 465     LYS A   366                                                      
REMARK 465     PRO A   367                                                      
REMARK 465     LEU A   368                                                      
REMARK 465     GLY A   369                                                      
REMARK 465     ASP A   370                                                      
REMARK 465     PHE A   371                                                      
REMARK 465     ASN A   372                                                      
REMARK 465     ASP A   373                                                      
REMARK 465     LEU A   374                                                      
REMARK 465     SER A   375                                                      
REMARK 465     GLN A   376                                                      
REMARK 465     LEU A   377                                                      
REMARK 465     VAL A   378                                                      
REMARK 465     LYS A   379                                                      
REMARK 465     LYS A   380                                                      
REMARK 465     LYS A   381                                                      
REMARK 465     PRO A   382                                                      
REMARK 465     ARG A   383                                                      
REMARK 465     ARG A   384                                                      
REMARK 465     HIS A   385                                                      
REMARK 465     LEU A   386                                                      
REMARK 465     GLU A   387                                                      
REMARK 465     HIS A   388                                                      
REMARK 465     HIS A   389                                                      
REMARK 465     HIS A   390                                                      
REMARK 465     HIS A   391                                                      
REMARK 465     HIS A   392                                                      
REMARK 465     HIS A   393                                                      
REMARK 465     MET B     1                                                      
REMARK 465     LYS B     2                                                      
REMARK 465     LEU B     3                                                      
REMARK 465     ARG B     4                                                      
REMARK 465     ALA B     5                                                      
REMARK 465     GLU B     6                                                      
REMARK 465     ASP B     7                                                      
REMARK 465     VAL B     8                                                      
REMARK 465     LEU B     9                                                      
REMARK 465     ALA B    10                                                      
REMARK 465     ASN B    11                                                      
REMARK 465     GLY B    12                                                      
REMARK 465     THR B    13                                                      
REMARK 465     SER B    14                                                      
REMARK 465     ARG B    15                                                      
REMARK 465     HIS B    16                                                      
REMARK 465     GLY B    84                                                      
REMARK 465     ASP B    85                                                      
REMARK 465     ALA B    86                                                      
REMARK 465     LEU B    87                                                      
REMARK 465     LEU B    88                                                      
REMARK 465     ALA B    89                                                      
REMARK 465     GLY B    90                                                      
REMARK 465     ASP B    91                                                      
REMARK 465     ASP B    92                                                      
REMARK 465     GLY B    93                                                      
REMARK 465     SER B    94                                                      
REMARK 465     GLY B    95                                                      
REMARK 465     SER B    96                                                      
REMARK 465     GLU B    97                                                      
REMARK 465     SER B    98                                                      
REMARK 465     GLU B    99                                                      
REMARK 465     SER B   100                                                      
REMARK 465     GLU B   101                                                      
REMARK 465     PRO B   102                                                      
REMARK 465     GLU B   103                                                      
REMARK 465     SER B   104                                                      
REMARK 465     ASP B   105                                                      
REMARK 465     VAL B   106                                                      
REMARK 465     SER B   107                                                      
REMARK 465     ASN B   108                                                      
REMARK 465     GLY B   109                                                      
REMARK 465     GLU B   110                                                      
REMARK 465     GLU B   111                                                      
REMARK 465     GLY B   112                                                      
REMARK 465     ASN B   113                                                      
REMARK 465     GLU B   114                                                      
REMARK 465     ASN B   115                                                      
REMARK 465     GLY B   116                                                      
REMARK 465     GLN B   117                                                      
REMARK 465     THR B   118                                                      
REMARK 465     GLU B   119                                                      
REMARK 465     ILE B   120                                                      
REMARK 465     PRO B   121                                                      
REMARK 465     ASN B   122                                                      
REMARK 465     SER B   123                                                      
REMARK 465     ARG B   124                                                      
REMARK 465     MET B   125                                                      
REMARK 465     PHE B   126                                                      
REMARK 465     GLN B   127                                                      
REMARK 465     PHE B   128                                                      
REMARK 465     ASP B   129                                                      
REMARK 465     GLN B   130                                                      
REMARK 465     GLU B   131                                                      
REMARK 465     GLU B   132                                                      
REMARK 465     GLU B   133                                                      
REMARK 465     ASP B   134                                                      
REMARK 465     LEU B   135                                                      
REMARK 465     THR B   136                                                      
REMARK 465     GLY B   137                                                      
REMARK 465     ASP B   138                                                      
REMARK 465     VAL B   139                                                      
REMARK 465     ASP B   140                                                      
REMARK 465     SER B   141                                                      
REMARK 465     GLY B   142                                                      
REMARK 465     ASP B   143                                                      
REMARK 465     SER B   144                                                      
REMARK 465     GLU B   145                                                      
REMARK 465     ASP B   146                                                      
REMARK 465     SER B   147                                                      
REMARK 465     GLY B   148                                                      
REMARK 465     GLU B   149                                                      
REMARK 465     GLY B   150                                                      
REMARK 465     SER B   151                                                      
REMARK 465     GLU B   152                                                      
REMARK 465     GLU B   153                                                      
REMARK 465     GLU B   154                                                      
REMARK 465     GLU B   155                                                      
REMARK 465     GLU B   156                                                      
REMARK 465     ASN B   157                                                      
REMARK 465     VAL B   158                                                      
REMARK 465     GLU B   159                                                      
REMARK 465     LYS B   160                                                      
REMARK 465     GLU B   161                                                      
REMARK 465     GLU B   162                                                      
REMARK 465     GLU B   163                                                      
REMARK 465     HIS B   346                                                      
REMARK 465     GLY B   347                                                      
REMARK 465     SER B   348                                                      
REMARK 465     LYS B   349                                                      
REMARK 465     ARG B   350                                                      
REMARK 465     PRO B   351                                                      
REMARK 465     LEU B   352                                                      
REMARK 465     SER B   353                                                      
REMARK 465     GLN B   354                                                      
REMARK 465     PRO B   355                                                      
REMARK 465     THR B   356                                                      
REMARK 465     THR B   357                                                      
REMARK 465     SER B   358                                                      
REMARK 465     ILE B   359                                                      
REMARK 465     GLY B   360                                                      
REMARK 465     PHE B   361                                                      
REMARK 465     PRO B   362                                                      
REMARK 465     ALA B   363                                                      
REMARK 465     LEU B   364                                                      
REMARK 465     GLU B   365                                                      
REMARK 465     LYS B   366                                                      
REMARK 465     PRO B   367                                                      
REMARK 465     LEU B   368                                                      
REMARK 465     GLY B   369                                                      
REMARK 465     ASP B   370                                                      
REMARK 465     PHE B   371                                                      
REMARK 465     ASN B   372                                                      
REMARK 465     ASP B   373                                                      
REMARK 465     LEU B   374                                                      
REMARK 465     SER B   375                                                      
REMARK 465     GLN B   376                                                      
REMARK 465     LEU B   377                                                      
REMARK 465     VAL B   378                                                      
REMARK 465     LYS B   379                                                      
REMARK 465     LYS B   380                                                      
REMARK 465     LYS B   381                                                      
REMARK 465     PRO B   382                                                      
REMARK 465     ARG B   383                                                      
REMARK 465     ARG B   384                                                      
REMARK 465     HIS B   385                                                      
REMARK 465     LEU B   386                                                      
REMARK 465     GLU B   387                                                      
REMARK 465     HIS B   388                                                      
REMARK 465     HIS B   389                                                      
REMARK 465     HIS B   390                                                      
REMARK 465     HIS B   391                                                      
REMARK 465     HIS B   392                                                      
REMARK 465     HIS B   393                                                      
REMARK 465     MET C   -11                                                      
REMARK 465     GLY C   -10                                                      
REMARK 465     HIS C    -9                                                      
REMARK 465     HIS C    -8                                                      
REMARK 465     HIS C    -7                                                      
REMARK 465     HIS C    -6                                                      
REMARK 465     HIS C    -5                                                      
REMARK 465     HIS C    -4                                                      
REMARK 465     GLY C    -3                                                      
REMARK 465     SER C    -2                                                      
REMARK 465     HIS C    -1                                                      
REMARK 465     MET C     0                                                      
REMARK 465     SER C     1                                                      
REMARK 465     GLY C     2                                                      
REMARK 465     GLY C     3                                                      
REMARK 465     LYS C     4                                                      
REMARK 465     GLY C     5                                                      
REMARK 465     GLY C     6                                                      
REMARK 465     LYS C     7                                                      
REMARK 465     ALA C     8                                                      
REMARK 465     GLY C     9                                                      
REMARK 465     SER C    10                                                      
REMARK 465     ALA C    11                                                      
REMARK 465     ALA C    12                                                      
REMARK 465     LYS C    13                                                      
REMARK 465     ALA C    14                                                      
REMARK 465     SER C    15                                                      
REMARK 465     VAL C   101                                                      
REMARK 465     THR C   102                                                      
REMARK 465     ILE C   103                                                      
REMARK 465     ALA C   104                                                      
REMARK 465     GLN C   105                                                      
REMARK 465     GLY C   106                                                      
REMARK 465     GLY C   107                                                      
REMARK 465     VAL C   108                                                      
REMARK 465     LEU C   109                                                      
REMARK 465     PRO C   110                                                      
REMARK 465     ASN C   111                                                      
REMARK 465     ILE C   112                                                      
REMARK 465     HIS C   113                                                      
REMARK 465     GLN C   114                                                      
REMARK 465     ASN C   115                                                      
REMARK 465     LEU C   116                                                      
REMARK 465     LEU C   117                                                      
REMARK 465     PRO C   118                                                      
REMARK 465     LYS C   119                                                      
REMARK 465     LYS C   120                                                      
REMARK 465     SER C   121                                                      
REMARK 465     ALA C   122                                                      
REMARK 465     LYS C   123                                                      
REMARK 465     ALA C   124                                                      
REMARK 465     THR C   125                                                      
REMARK 465     LYS C   126                                                      
REMARK 465     ALA C   127                                                      
REMARK 465     SER C   128                                                      
REMARK 465     GLN C   129                                                      
REMARK 465     GLU C   130                                                      
REMARK 465     LEU C   131                                                      
REMARK 465     MET D   -11                                                      
REMARK 465     GLY D   -10                                                      
REMARK 465     HIS D    -9                                                      
REMARK 465     HIS D    -8                                                      
REMARK 465     HIS D    -7                                                      
REMARK 465     HIS D    -6                                                      
REMARK 465     HIS D    -5                                                      
REMARK 465     HIS D    -4                                                      
REMARK 465     GLY D    -3                                                      
REMARK 465     SER D    -2                                                      
REMARK 465     HIS D    -1                                                      
REMARK 465     MET D     0                                                      
REMARK 465     SER D     1                                                      
REMARK 465     ALA D     2                                                      
REMARK 465     LYS D     3                                                      
REMARK 465     ALA D     4                                                      
REMARK 465     GLU D     5                                                      
REMARK 465     LYS D     6                                                      
REMARK 465     LYS D     7                                                      
REMARK 465     PRO D     8                                                      
REMARK 465     ALA D     9                                                      
REMARK 465     SER D    10                                                      
REMARK 465     LYS D    11                                                      
REMARK 465     ALA D    12                                                      
REMARK 465     PRO D    13                                                      
REMARK 465     ALA D    14                                                      
REMARK 465     GLU D    15                                                      
REMARK 465     LYS D    16                                                      
REMARK 465     LYS D    17                                                      
REMARK 465     PRO D    18                                                      
REMARK 465     ALA D    19                                                      
REMARK 465     ALA D    20                                                      
REMARK 465     LYS D    21                                                      
REMARK 465     LYS D    22                                                      
REMARK 465     THR D    23                                                      
REMARK 465     SER D    24                                                      
REMARK 465     THR D    25                                                      
REMARK 465     SER D    26                                                      
REMARK 465     THR D    27                                                      
REMARK 465     ASP D    28                                                      
REMARK 465     GLY D    29                                                      
REMARK 465     LYS D    30                                                      
REMARK 465     LYS D    31                                                      
REMARK 465     ARG D    32                                                      
REMARK 465     SER D    33                                                      
REMARK 465     LYS D    34                                                      
REMARK 465     ALA D    35                                                      
REMARK 465     ARG D    36                                                      
REMARK 465     SER D   127                                                      
REMARK 465     THR D   128                                                      
REMARK 465     GLN D   129                                                      
REMARK 465     ALA D   130                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A  15    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     HIS A  16    CG   ND1  CD2  CE1  NE2                             
REMARK 470     HIS A 168    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ASP A 181    CG   OD1  OD2                                       
REMARK 470     GLU A 204    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 254    CZ   NH1  NH2                                       
REMARK 470     LYS A 270    CG   CD   CE   NZ                                   
REMARK 470     LYS A 299    CG   CD   CE   NZ                                   
REMARK 470     GLU A 342    CG   CD   OE1  OE2                                  
REMARK 470     GLN A 344    CG   CD   OE1  NE2                                  
REMARK 470     GLN A 345    CG   CD   OE1  NE2                                  
REMARK 470     HIS A 346    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LYS B  17    CG   CD   CE   NZ                                   
REMARK 470     VAL B  18    CG1  CG2                                            
REMARK 470     LYS B  54    CD   CE   NZ                                        
REMARK 470     SER B  79    OG                                                  
REMARK 470     LYS B 270    CG   CD   CE   NZ                                   
REMARK 470     GLN B 274    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 324    CD   OE1  OE2                                       
REMARK 470     GLN B 345    CG   CD   OE1  NE2                                  
REMARK 470     GLU C  65    CD   OE1  OE2                                       
REMARK 470     LYS C  75    CG   CD   CE   NZ                                   
REMARK 470     ARG C  82    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS D  88    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A  17       90.70   -165.83                                   
REMARK 500    LYS A  36       17.58     59.84                                   
REMARK 500    ASN A 200     -137.61     59.46                                   
REMARK 500    ASP A 322       40.81   -108.07                                   
REMARK 500    ASN A 343       35.92    -77.81                                   
REMARK 500    GLN A 345       -8.01     66.58                                   
REMARK 500    ASN B 200     -128.02     50.07                                   
REMARK 500    MET B 251       71.54   -100.09                                   
REMARK 500    THR D  51      -63.07    -96.03                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4NQ0   RELATED DB: PDB                                   
REMARK 900 FREE HIF1 WITHOUT HISTONE BINDING                                    
DBREF  5BT1 A    1   385  UNP    Q12373   HIF1_YEAST       1    385             
DBREF  5BT1 B    1   385  UNP    Q12373   HIF1_YEAST       1    385             
DBREF  5BT1 C    0   131  UNP    P04911   H2A1_YEAST       1    132             
DBREF  5BT1 D    0   130  UNP    P02293   H2B1_YEAST       1    131             
SEQADV 5BT1 LEU A  386  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 GLU A  387  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS A  388  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS A  389  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS A  390  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS A  391  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS A  392  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS A  393  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 LEU B  386  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 GLU B  387  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS B  388  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS B  389  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS B  390  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS B  391  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS B  392  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 HIS B  393  UNP  Q12373              EXPRESSION TAG                 
SEQADV 5BT1 MET C  -11  UNP  P04911              INITIATING METHIONINE          
SEQADV 5BT1 GLY C  -10  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -9  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -8  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -7  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -6  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -5  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -4  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 GLY C   -3  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 SER C   -2  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 HIS C   -1  UNP  P04911              EXPRESSION TAG                 
SEQADV 5BT1 MET D  -11  UNP  P02293              INITIATING METHIONINE          
SEQADV 5BT1 GLY D  -10  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -9  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -8  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -7  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -6  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -5  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -4  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 GLY D   -3  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 SER D   -2  UNP  P02293              EXPRESSION TAG                 
SEQADV 5BT1 HIS D   -1  UNP  P02293              EXPRESSION TAG                 
SEQRES   1 A  393  MET LYS LEU ARG ALA GLU ASP VAL LEU ALA ASN GLY THR          
SEQRES   2 A  393  SER ARG HIS LYS VAL GLN ILE ASP MET GLU ARG GLN VAL          
SEQRES   3 A  393  GLN ILE ALA LYS ASP LEU LEU ALA GLN LYS LYS PHE LEU          
SEQRES   4 A  393  GLU ALA ALA LYS ARG CYS GLN GLN THR LEU ASP SER LEU          
SEQRES   5 A  393  PRO LYS ASP GLY LEU LEU PRO ASP PRO GLU LEU PHE THR          
SEQRES   6 A  393  ILE PHE ALA GLN ALA VAL TYR ASN MET GLU VAL GLN ASN          
SEQRES   7 A  393  SER GLY ASN LEU PHE GLY ASP ALA LEU LEU ALA GLY ASP          
SEQRES   8 A  393  ASP GLY SER GLY SER GLU SER GLU SER GLU PRO GLU SER          
SEQRES   9 A  393  ASP VAL SER ASN GLY GLU GLU GLY ASN GLU ASN GLY GLN          
SEQRES  10 A  393  THR GLU ILE PRO ASN SER ARG MET PHE GLN PHE ASP GLN          
SEQRES  11 A  393  GLU GLU GLU ASP LEU THR GLY ASP VAL ASP SER GLY ASP          
SEQRES  12 A  393  SER GLU ASP SER GLY GLU GLY SER GLU GLU GLU GLU GLU          
SEQRES  13 A  393  ASN VAL GLU LYS GLU GLU GLU ARG LEU ALA LEU HIS GLU          
SEQRES  14 A  393  LEU ALA ASN PHE SER PRO ALA ASN GLU HIS ASP ASP GLU          
SEQRES  15 A  393  ILE GLU ASP VAL SER GLN LEU ARG LYS SER GLY PHE HIS          
SEQRES  16 A  393  ILE TYR PHE GLU ASN ASP LEU TYR GLU ASN ALA LEU ASP          
SEQRES  17 A  393  LEU LEU ALA GLN ALA LEU MET LEU LEU GLY ARG PRO THR          
SEQRES  18 A  393  ALA ASP GLY GLN SER LEU THR GLU ASN SER ARG LEU ARG          
SEQRES  19 A  393  ILE GLY ASP VAL TYR ILE LEU MET GLY ASP ILE GLU ARG          
SEQRES  20 A  393  GLU ALA GLU MET PHE SER ARG ALA ILE HIS HIS TYR LEU          
SEQRES  21 A  393  LYS ALA LEU GLY TYR TYR LYS THR LEU LYS PRO ALA GLU          
SEQRES  22 A  393  GLN VAL THR GLU LYS VAL ILE GLN ALA GLU PHE LEU VAL          
SEQRES  23 A  393  CYS ASP ALA LEU ARG TRP VAL ASP GLN VAL PRO ALA LYS          
SEQRES  24 A  393  ASP LYS LEU LYS ARG PHE LYS HIS ALA LYS ALA LEU LEU          
SEQRES  25 A  393  GLU LYS HIS MET THR THR ARG PRO LYS ASP SER GLU LEU          
SEQRES  26 A  393  GLN GLN ALA ARG LEU ALA GLN ILE GLN ASP ASP ILE ASP          
SEQRES  27 A  393  GLU VAL GLN GLU ASN GLN GLN HIS GLY SER LYS ARG PRO          
SEQRES  28 A  393  LEU SER GLN PRO THR THR SER ILE GLY PHE PRO ALA LEU          
SEQRES  29 A  393  GLU LYS PRO LEU GLY ASP PHE ASN ASP LEU SER GLN LEU          
SEQRES  30 A  393  VAL LYS LYS LYS PRO ARG ARG HIS LEU GLU HIS HIS HIS          
SEQRES  31 A  393  HIS HIS HIS                                                  
SEQRES   1 B  393  MET LYS LEU ARG ALA GLU ASP VAL LEU ALA ASN GLY THR          
SEQRES   2 B  393  SER ARG HIS LYS VAL GLN ILE ASP MET GLU ARG GLN VAL          
SEQRES   3 B  393  GLN ILE ALA LYS ASP LEU LEU ALA GLN LYS LYS PHE LEU          
SEQRES   4 B  393  GLU ALA ALA LYS ARG CYS GLN GLN THR LEU ASP SER LEU          
SEQRES   5 B  393  PRO LYS ASP GLY LEU LEU PRO ASP PRO GLU LEU PHE THR          
SEQRES   6 B  393  ILE PHE ALA GLN ALA VAL TYR ASN MET GLU VAL GLN ASN          
SEQRES   7 B  393  SER GLY ASN LEU PHE GLY ASP ALA LEU LEU ALA GLY ASP          
SEQRES   8 B  393  ASP GLY SER GLY SER GLU SER GLU SER GLU PRO GLU SER          
SEQRES   9 B  393  ASP VAL SER ASN GLY GLU GLU GLY ASN GLU ASN GLY GLN          
SEQRES  10 B  393  THR GLU ILE PRO ASN SER ARG MET PHE GLN PHE ASP GLN          
SEQRES  11 B  393  GLU GLU GLU ASP LEU THR GLY ASP VAL ASP SER GLY ASP          
SEQRES  12 B  393  SER GLU ASP SER GLY GLU GLY SER GLU GLU GLU GLU GLU          
SEQRES  13 B  393  ASN VAL GLU LYS GLU GLU GLU ARG LEU ALA LEU HIS GLU          
SEQRES  14 B  393  LEU ALA ASN PHE SER PRO ALA ASN GLU HIS ASP ASP GLU          
SEQRES  15 B  393  ILE GLU ASP VAL SER GLN LEU ARG LYS SER GLY PHE HIS          
SEQRES  16 B  393  ILE TYR PHE GLU ASN ASP LEU TYR GLU ASN ALA LEU ASP          
SEQRES  17 B  393  LEU LEU ALA GLN ALA LEU MET LEU LEU GLY ARG PRO THR          
SEQRES  18 B  393  ALA ASP GLY GLN SER LEU THR GLU ASN SER ARG LEU ARG          
SEQRES  19 B  393  ILE GLY ASP VAL TYR ILE LEU MET GLY ASP ILE GLU ARG          
SEQRES  20 B  393  GLU ALA GLU MET PHE SER ARG ALA ILE HIS HIS TYR LEU          
SEQRES  21 B  393  LYS ALA LEU GLY TYR TYR LYS THR LEU LYS PRO ALA GLU          
SEQRES  22 B  393  GLN VAL THR GLU LYS VAL ILE GLN ALA GLU PHE LEU VAL          
SEQRES  23 B  393  CYS ASP ALA LEU ARG TRP VAL ASP GLN VAL PRO ALA LYS          
SEQRES  24 B  393  ASP LYS LEU LYS ARG PHE LYS HIS ALA LYS ALA LEU LEU          
SEQRES  25 B  393  GLU LYS HIS MET THR THR ARG PRO LYS ASP SER GLU LEU          
SEQRES  26 B  393  GLN GLN ALA ARG LEU ALA GLN ILE GLN ASP ASP ILE ASP          
SEQRES  27 B  393  GLU VAL GLN GLU ASN GLN GLN HIS GLY SER LYS ARG PRO          
SEQRES  28 B  393  LEU SER GLN PRO THR THR SER ILE GLY PHE PRO ALA LEU          
SEQRES  29 B  393  GLU LYS PRO LEU GLY ASP PHE ASN ASP LEU SER GLN LEU          
SEQRES  30 B  393  VAL LYS LYS LYS PRO ARG ARG HIS LEU GLU HIS HIS HIS          
SEQRES  31 B  393  HIS HIS HIS                                                  
SEQRES   1 C  143  MET GLY HIS HIS HIS HIS HIS HIS GLY SER HIS MET SER          
SEQRES   2 C  143  GLY GLY LYS GLY GLY LYS ALA GLY SER ALA ALA LYS ALA          
SEQRES   3 C  143  SER GLN SER ARG SER ALA LYS ALA GLY LEU THR PHE PRO          
SEQRES   4 C  143  VAL GLY ARG VAL HIS ARG LEU LEU ARG ARG GLY ASN TYR          
SEQRES   5 C  143  ALA GLN ARG ILE GLY SER GLY ALA PRO VAL TYR LEU THR          
SEQRES   6 C  143  ALA VAL LEU GLU TYR LEU ALA ALA GLU ILE LEU GLU LEU          
SEQRES   7 C  143  ALA GLY ASN ALA ALA ARG ASP ASN LYS LYS THR ARG ILE          
SEQRES   8 C  143  ILE PRO ARG HIS LEU GLN LEU ALA ILE ARG ASN ASP ASP          
SEQRES   9 C  143  GLU LEU ASN LYS LEU LEU GLY ASN VAL THR ILE ALA GLN          
SEQRES  10 C  143  GLY GLY VAL LEU PRO ASN ILE HIS GLN ASN LEU LEU PRO          
SEQRES  11 C  143  LYS LYS SER ALA LYS ALA THR LYS ALA SER GLN GLU LEU          
SEQRES   1 D  142  MET GLY HIS HIS HIS HIS HIS HIS GLY SER HIS MET SER          
SEQRES   2 D  142  ALA LYS ALA GLU LYS LYS PRO ALA SER LYS ALA PRO ALA          
SEQRES   3 D  142  GLU LYS LYS PRO ALA ALA LYS LYS THR SER THR SER THR          
SEQRES   4 D  142  ASP GLY LYS LYS ARG SER LYS ALA ARG LYS GLU THR TYR          
SEQRES   5 D  142  SER SER TYR ILE TYR LYS VAL LEU LYS GLN THR HIS PRO          
SEQRES   6 D  142  ASP THR GLY ILE SER GLN LYS SER MET SER ILE LEU ASN          
SEQRES   7 D  142  SER PHE VAL ASN ASP ILE PHE GLU ARG ILE ALA THR GLU          
SEQRES   8 D  142  ALA SER LYS LEU ALA ALA TYR ASN LYS LYS SER THR ILE          
SEQRES   9 D  142  SER ALA ARG GLU ILE GLN THR ALA VAL ARG LEU ILE LEU          
SEQRES  10 D  142  PRO GLY GLU LEU ALA LYS HIS ALA VAL SER GLU GLY THR          
SEQRES  11 D  142  ARG ALA VAL THR LYS TYR SER SER SER THR GLN ALA              
FORMUL   5  HOH   *47(H2 O)                                                     
HELIX    1 AA1 ASP A   21  GLN A   35  1                                  15    
HELIX    2 AA2 LYS A   37  LEU A   52  1                                  16    
HELIX    3 AA3 PRO A   61  ASN A   78  1                                  18    
HELIX    4 AA4 ASP A  185  LEU A  189  5                                   5    
HELIX    5 AA5 GLY A  193  GLU A  199  5                                   7    
HELIX    6 AA6 ASP A  201  GLY A  218  1                                  18    
HELIX    7 AA7 THR A  228  GLU A  250  1                                  23    
HELIX    8 AA8 MET A  251  LYS A  267  1                                  17    
HELIX    9 AA9 ALA A  272  ARG A  291  1                                  20    
HELIX   10 AB1 PRO A  297  THR A  317  1                                  21    
HELIX   11 AB2 ASP A  322  ASN A  343  1                                  22    
HELIX   12 AB3 ASP B   21  GLN B   35  1                                  15    
HELIX   13 AB4 LYS B   37  LEU B   52  1                                  16    
HELIX   14 AB5 PRO B   61  GLY B   80  1                                  20    
HELIX   15 AB6 ALA B  166  ASN B  172  1                                   7    
HELIX   16 AB7 ASP B  185  LEU B  189  5                                   5    
HELIX   17 AB8 GLY B  193  GLU B  199  5                                   7    
HELIX   18 AB9 ASP B  201  GLY B  218  1                                  18    
HELIX   19 AC1 THR B  228  ALA B  249  1                                  22    
HELIX   20 AC2 MET B  251  LYS B  267  1                                  17    
HELIX   21 AC3 ALA B  272  LEU B  290  1                                  19    
HELIX   22 AC4 ARG B  291  VAL B  293  5                                   3    
HELIX   23 AC5 PRO B  297  THR B  317  1                                  21    
HELIX   24 AC6 ASP B  322  ASN B  343  1                                  22    
HELIX   25 AC7 SER C   17  GLY C   23  1                                   7    
HELIX   26 AC8 PRO C   27  ARG C   37  1                                  11    
HELIX   27 AC9 SER C   46  ASN C   74  1                                  29    
HELIX   28 AD1 ILE C   80  ASN C   90  1                                  11    
HELIX   29 AD2 ASP C   91  GLY C   99  1                                   9    
HELIX   30 AD3 TYR D   40  HIS D   52  1                                  13    
HELIX   31 AD4 SER D   58  ASN D   87  1                                  30    
HELIX   32 AD5 SER D   93  LEU D  105  1                                  13    
HELIX   33 AD6 PRO D  106  SER D  126  1                                  21    
SHEET    1 AA1 2 ARG C  43  ILE C  44  0                                        
SHEET    2 AA1 2 THR D  91  ILE D  92  1  O  ILE D  92   N  ARG C  43           
SHEET    1 AA2 2 ARG C  78  ILE C  79  0                                        
SHEET    2 AA2 2 GLY D  56  ILE D  57  1  O  GLY D  56   N  ILE C  79           
CRYST1  102.673   48.363  160.706  90.00 107.05  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009740  0.000000  0.002986        0.00000                         
SCALE2      0.000000  0.020677  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.006508        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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