GenomeNet

Database: PDB
Entry: 5ECJ
LinkDB: 5ECJ
Original site: 5ECJ 
HEADER    GENE REGULATION/TRANSCRIPTION           20-OCT-15   5ECJ              
TITLE     CRYSTAL STRUCTURE OF MONOBODY MB(S4) BOUND TO PRDM14 IN COMPLEX WITH  
TITLE    2 MTGR1                                                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PR DOMAIN ZINC FINGER PROTEIN 14,PROTEIN CBFA2T2;          
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: PR DOMAIN-CONTAINING PROTEIN 14,MTG8-LIKE PROTEIN,MTG8-     
COMPND   5 RELATED PROTEIN 1;                                                   
COMPND   6 EC: 2.1.1.-;                                                         
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MUTATION: YES;                                                       
COMPND   9 MOL_ID: 2;                                                           
COMPND  10 MOLECULE: MONOBODY MB(S4);                                           
COMPND  11 CHAIN: F, E;                                                         
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: MOUSE;                                              
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 GENE: PRDM14, CBFA2T2, CBFA2T2H, MTGR1;                              
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  15 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)                                  
KEYWDS    TRANSFERASE, PROTEIN BINDING, GENE REGULATION-TRANSCRIPTION COMPLEX   
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    A.GUPTA,S.KOIDE                                                       
REVDAT   3   20-SEP-17 5ECJ    1       REMARK                                   
REVDAT   2   04-MAY-16 5ECJ    1       JRNL                                     
REVDAT   1   02-DEC-15 5ECJ    0                                                
JRNL        AUTH   N.NADY,A.GUPTA,Z.MA,T.SWIGUT,A.KOIDE,S.KOIDE,J.WYSOCKA       
JRNL        TITL   ETO FAMILY PROTEIN MTGR1 MEDIATES PRDM14 FUNCTIONS IN STEM   
JRNL        TITL 2 CELL MAINTENANCE AND PRIMORDIAL GERM CELL FORMATION.         
JRNL        REF    ELIFE                         V.   4 10150 2015              
JRNL        REFN                   ESSN 2050-084X                               
JRNL        PMID   26523391                                                     
JRNL        DOI    10.7554/ELIFE.10150                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.05 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.9_1692                                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.05                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 37.76                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 20581                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.194                           
REMARK   3   R VALUE            (WORKING SET) : 0.189                           
REMARK   3   FREE R VALUE                     : 0.250                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 8.380                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1725                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 37.7666 -  6.9694    0.98     1687   157  0.1838 0.2185        
REMARK   3     2  6.9694 -  5.5374    1.00     1621   148  0.2015 0.2553        
REMARK   3     3  5.5374 -  4.8391    1.00     1588   145  0.1575 0.2140        
REMARK   3     4  4.8391 -  4.3974    1.00     1583   145  0.1487 0.2032        
REMARK   3     5  4.3974 -  4.0826    1.00     1561   143  0.1555 0.2575        
REMARK   3     6  4.0826 -  3.8421    1.00     1550   141  0.1851 0.2317        
REMARK   3     7  3.8421 -  3.6499    1.00     1574   143  0.2009 0.2806        
REMARK   3     8  3.6499 -  3.4911    1.00     1544   141  0.2219 0.3365        
REMARK   3     9  3.4911 -  3.3568    1.00     1536   141  0.2494 0.3142        
REMARK   3    10  3.3568 -  3.2410    1.00     1536   139  0.2661 0.4156        
REMARK   3    11  3.2410 -  3.1397    1.00     1529   140  0.2866 0.3832        
REMARK   3    12  3.1397 -  3.0500    1.00     1547   142  0.2834 0.3469        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.430            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.910           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006           5617                                  
REMARK   3   ANGLE     :  1.074           7648                                  
REMARK   3   CHIRALITY :  0.042            856                                  
REMARK   3   PLANARITY :  0.006            991                                  
REMARK   3   DIHEDRAL  : 13.665           1972                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 27                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 2 THROUGH 26 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.3658  25.5703   5.6303              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   2.1324 T22:   0.6093                                     
REMARK   3      T33:   1.1654 T12:   0.1440                                     
REMARK   3      T13:  -0.1446 T23:   0.1000                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.6097 L22:   3.5889                                     
REMARK   3      L33:   5.7687 L12:  -1.7366                                     
REMARK   3      L13:  -0.4512 L23:   4.2404                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2749 S12:   0.2276 S13:  -1.3423                       
REMARK   3      S21:   3.2876 S22:  -0.4621 S23:   1.6119                       
REMARK   3      S31:   0.8622 S32:  -0.3627 S33:   0.5636                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 27 THROUGH 129 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):   5.9322  43.6038  -1.8187              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.9139 T22:   0.9215                                     
REMARK   3      T33:   1.0122 T12:   0.0012                                     
REMARK   3      T13:  -0.2615 T23:  -0.0098                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.2170 L22:   5.3352                                     
REMARK   3      L33:   5.8059 L12:  -0.5140                                     
REMARK   3      L13:   0.8575 L23:   0.0283                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0062 S12:   0.2243 S13:  -0.1775                       
REMARK   3      S21:   0.8862 S22:  -0.1323 S23:  -1.0184                       
REMARK   3      S31:  -0.0758 S32:   1.2983 S33:   0.2328                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 130 THROUGH 225 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.9248  39.7721  -7.7071              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8253 T22:   0.7441                                     
REMARK   3      T33:   0.5964 T12:  -0.0215                                     
REMARK   3      T13:  -0.2256 T23:  -0.0406                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.1326 L22:   6.2130                                     
REMARK   3      L33:   2.9354 L12:  -0.1585                                     
REMARK   3      L13:  -1.7299 L23:   0.4718                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0716 S12:   0.3324 S13:  -0.3324                       
REMARK   3      S21:   0.0204 S22:   0.3349 S23:   0.1259                       
REMARK   3      S31:  -0.4251 S32:  -0.0509 S33:  -0.2938                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 226 THROUGH 288 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.1397  20.5093 -11.6280              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0749 T22:   0.9070                                     
REMARK   3      T33:   1.2470 T12:   0.3073                                     
REMARK   3      T13:  -0.2023 T23:  -0.4063                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5514 L22:   6.2303                                     
REMARK   3      L33:   3.3429 L12:   1.6988                                     
REMARK   3      L13:   0.4305 L23:  -0.4030                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1733 S12:   0.5099 S13:  -1.5569                       
REMARK   3      S21:  -0.0433 S22:   0.2310 S23:  -0.5004                       
REMARK   3      S31:   0.8197 S32:   0.3241 S33:  -0.2000                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 1 THROUGH 8 )                     
REMARK   3    ORIGIN FOR THE GROUP (A): -25.5374  13.4454  26.1752              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8536 T22:   1.4956                                     
REMARK   3      T33:   1.0152 T12:  -0.3854                                     
REMARK   3      T13:  -0.8712 T23:  -0.2022                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.5144 L22:   5.0398                                     
REMARK   3      L33:   4.5862 L12:   0.1721                                     
REMARK   3      L13:   0.8364 L23:   3.1664                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6555 S12:  -0.4146 S13:  -0.4058                       
REMARK   3      S21:  -1.4402 S22:   0.8263 S23:   0.4334                       
REMARK   3      S31:   0.3524 S32:  -0.2115 S33:   0.9748                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 9 THROUGH 28 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -29.2546   1.8825  19.9773              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.5185 T22:   1.2961                                     
REMARK   3      T33:   0.8693 T12:  -0.2682                                     
REMARK   3      T13:   0.0210 T23:   0.2323                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.1589 L22:   2.2721                                     
REMARK   3      L33:   3.9015 L12:   2.2781                                     
REMARK   3      L13:   1.0894 L23:  -0.4442                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0276 S12:  -0.8152 S13:  -0.0951                       
REMARK   3      S21:   2.0783 S22:  -0.3760 S23:  -0.4817                       
REMARK   3      S31:   0.3080 S32:   0.7644 S33:   0.5550                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 29 THROUGH 51 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -25.8466   8.4636  10.7317              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0110 T22:   1.1875                                     
REMARK   3      T33:   1.2560 T12:  -0.0997                                     
REMARK   3      T13:   0.0011 T23:   0.1238                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.4895 L22:   2.0584                                     
REMARK   3      L33:   2.0552 L12:  -1.1073                                     
REMARK   3      L13:  -3.2618 L23:   0.6737                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4279 S12:  -0.2589 S13:  -0.2975                       
REMARK   3      S21:  -0.2726 S22:  -0.4582 S23:  -1.4421                       
REMARK   3      S31:  -1.2060 S32:   0.6220 S33:  -0.0734                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 52 THROUGH 77 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -27.0327   5.6837  14.5363              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0147 T22:   1.4697                                     
REMARK   3      T33:   0.7780 T12:  -0.3399                                     
REMARK   3      T13:  -0.0142 T23:   0.1166                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.1942 L22:   2.0618                                     
REMARK   3      L33:   8.8129 L12:   1.0815                                     
REMARK   3      L13:   1.1408 L23:  -0.0226                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5917 S12:  -1.4590 S13:  -0.2882                       
REMARK   3      S21:   0.2377 S22:  -0.4430 S23:  -0.8267                       
REMARK   3      S31:  -1.8134 S32:   0.0649 S33:  -0.1213                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 78 THROUGH 93 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -23.7033  11.3935  18.0420              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7512 T22:   1.5132                                     
REMARK   3      T33:   0.8743 T12:  -0.4823                                     
REMARK   3      T13:  -0.3599 T23:   0.0084                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.4507 L22:   5.3035                                     
REMARK   3      L33:   9.0816 L12:   0.2243                                     
REMARK   3      L13:   0.9132 L23:  -0.8008                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.8204 S12:  -1.3868 S13:  -0.7367                       
REMARK   3      S21:   0.7163 S22:  -0.9831 S23:   0.4233                       
REMARK   3      S31:  -2.1607 S32:  -0.2891 S33:   0.3124                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 2 THROUGH 14 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -31.8716  44.7052 -14.2214              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.6426 T22:   0.6690                                     
REMARK   3      T33:   1.6052 T12:   0.0334                                     
REMARK   3      T13:  -0.2448 T23:  -0.0393                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5712 L22:   7.1373                                     
REMARK   3      L33:   7.3113 L12:  -4.5370                                     
REMARK   3      L13:   6.1732 L23:  -3.8977                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4631 S12:   0.4330 S13:   0.4417                       
REMARK   3      S21:   3.1028 S22:   0.1340 S23:   0.9182                       
REMARK   3      S31:  -1.5363 S32:   1.0044 S33:   0.3789                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 15 THROUGH 53 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -40.0184  39.4963   3.0356              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.5137 T22:   1.3355                                     
REMARK   3      T33:   1.2284 T12:   0.3104                                     
REMARK   3      T13:  -0.2170 T23:  -0.4087                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.8257 L22:   6.8085                                     
REMARK   3      L33:   4.4704 L12:   4.3946                                     
REMARK   3      L13:  -2.8728 L23:  -5.2493                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0132 S12:  -1.8060 S13:   1.6758                       
REMARK   3      S21:   0.1494 S22:  -0.8153 S23:   0.8054                       
REMARK   3      S31:  -0.6371 S32:   0.8462 S33:   1.2232                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 54 THROUGH 77 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -43.1610  35.9499   5.7651              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8546 T22:   1.4354                                     
REMARK   3      T33:   1.3971 T12:   0.3998                                     
REMARK   3      T13:   0.0285 T23:  -0.4726                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7646 L22:   4.4562                                     
REMARK   3      L33:   2.7242 L12:  -3.0561                                     
REMARK   3      L13:   2.1857 L23:  -1.2299                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2959 S12:  -1.4126 S13:   2.1150                       
REMARK   3      S21:  -0.2481 S22:  -0.3414 S23:   0.0626                       
REMARK   3      S31:  -0.1670 S32:  -2.2736 S33:   0.8608                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 78 THROUGH 150 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -39.1670  22.1505  -2.0152              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1160 T22:   0.8076                                     
REMARK   3      T33:   0.7613 T12:   0.1036                                     
REMARK   3      T13:   0.0231 T23:  -0.2250                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.8712 L22:   5.6818                                     
REMARK   3      L33:   6.9133 L12:   1.3513                                     
REMARK   3      L13:   1.6329 L23:  -0.7419                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2629 S12:  -0.9064 S13:   0.0891                       
REMARK   3      S21:   1.0455 S22:  -0.1032 S23:   0.1954                       
REMARK   3      S31:  -0.3031 S32:  -0.7547 S33:  -0.1843                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 151 THROUGH 181 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -30.4300  24.3128   0.6899              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.2787 T22:   0.6691                                     
REMARK   3      T33:   0.8583 T12:  -0.1799                                     
REMARK   3      T13:  -0.1711 T23:  -0.2580                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.5428 L22:   1.3302                                     
REMARK   3      L33:   2.4820 L12:  -1.0728                                     
REMARK   3      L13:  -2.0794 L23:  -0.1856                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0716 S12:  -0.6913 S13:   0.1340                       
REMARK   3      S21:   1.7243 S22:  -0.0557 S23:  -0.5908                       
REMARK   3      S31:  -0.7201 S32:   0.4779 S33:   0.0049                       
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 182 THROUGH 206 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -32.1733  15.2454 -10.3667              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.9671 T22:   0.6626                                     
REMARK   3      T33:   0.8448 T12:  -0.1157                                     
REMARK   3      T13:  -0.2131 T23:  -0.0607                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.3092 L22:   6.3357                                     
REMARK   3      L33:   6.2051 L12:  -1.8801                                     
REMARK   3      L13:   0.7040 L23:  -2.1033                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2867 S12:   0.1320 S13:  -0.6462                       
REMARK   3      S21:  -0.7625 S22:   0.6923 S23:   0.5383                       
REMARK   3      S31:   1.1151 S32:  -0.0785 S33:  -0.4011                       
REMARK   3   TLS GROUP : 16                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 207 THROUGH 263 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -24.2218  34.2110 -20.1984              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8656 T22:   0.8528                                     
REMARK   3      T33:   0.9671 T12:   0.0329                                     
REMARK   3      T13:  -0.1878 T23:  -0.2118                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.9652 L22:   4.7971                                     
REMARK   3      L33:   5.2653 L12:  -0.5407                                     
REMARK   3      L13:   1.6618 L23:  -3.4470                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1562 S12:   0.8436 S13:   0.5999                       
REMARK   3      S21:   0.3311 S22:   0.0494 S23:  -0.6397                       
REMARK   3      S31:   0.2375 S32:   0.9430 S33:   0.1492                       
REMARK   3   TLS GROUP : 17                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 264 THROUGH 286 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -37.7210  34.6712 -18.0263              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.9299 T22:   1.0112                                     
REMARK   3      T33:   1.0233 T12:   0.2533                                     
REMARK   3      T13:  -0.1786 T23:  -0.0588                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.3815 L22:   4.6447                                     
REMARK   3      L33:   8.3628 L12:  -0.7061                                     
REMARK   3      L13:  -0.8999 L23:   6.4405                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5550 S12:   0.7700 S13:  -0.3712                       
REMARK   3      S21:  -0.3933 S22:   0.1314 S23:   2.0136                       
REMARK   3      S31:   0.1500 S32:  -0.5106 S33:   0.1701                       
REMARK   3   TLS GROUP : 18                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 1 THROUGH 8 )                     
REMARK   3    ORIGIN FOR THE GROUP (A): -15.5237  69.3784   0.6217              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1172 T22:   0.9281                                     
REMARK   3      T33:   1.2121 T12:   0.3174                                     
REMARK   3      T13:   0.0694 T23:  -0.2272                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.4610 L22:   2.2845                                     
REMARK   3      L33:   6.0284 L12:  -1.2159                                     
REMARK   3      L13:  -0.2812 L23:   3.3472                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3696 S12:  -0.4856 S13:   0.0369                       
REMARK   3      S21:   0.4082 S22:  -0.2068 S23:   2.7588                       
REMARK   3      S31:  -1.5278 S32:  -0.3161 S33:   0.4498                       
REMARK   3   TLS GROUP : 19                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 9 THROUGH 14 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -11.5331  73.8233 -18.0498              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8420 T22:   0.9295                                     
REMARK   3      T33:   0.7434 T12:  -0.0468                                     
REMARK   3      T13:  -0.1390 T23:   0.1416                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.0207 L22:   2.6196                                     
REMARK   3      L33:   2.7115 L12:   1.1951                                     
REMARK   3      L13:  -1.3178 L23:  -2.6623                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2410 S12:   0.8336 S13:  -0.2054                       
REMARK   3      S21:  -1.1055 S22:   0.1816 S23:   1.8442                       
REMARK   3      S31:   0.1311 S32:   0.1911 S33:   0.5622                       
REMARK   3   TLS GROUP : 20                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 15 THROUGH 21 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -9.5567  70.3406 -18.5881              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.9757 T22:   0.6030                                     
REMARK   3      T33:   0.6740 T12:  -0.2034                                     
REMARK   3      T13:  -0.3208 T23:   0.0389                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.2886 L22:   8.7167                                     
REMARK   3      L33:   2.4738 L12:  -2.2621                                     
REMARK   3      L13:  -2.8228 L23:  -2.9106                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1987 S12:   1.0521 S13:   0.1547                       
REMARK   3      S21:  -1.1796 S22:   0.2292 S23:   0.4662                       
REMARK   3      S31:   0.7490 S32:  -0.1528 S33:   0.0414                       
REMARK   3   TLS GROUP : 21                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 22 THROUGH 38 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -9.8718  65.0216  -4.4378              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3097 T22:   0.6884                                     
REMARK   3      T33:   0.6066 T12:   0.1984                                     
REMARK   3      T13:  -0.0345 T23:   0.0132                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.6769 L22:   6.1769                                     
REMARK   3      L33:   6.5738 L12:   4.7958                                     
REMARK   3      L13:   6.6948 L23:   5.8711                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6064 S12:  -0.5047 S13:   1.0555                       
REMARK   3      S21:   1.2428 S22:  -0.0519 S23:   0.4369                       
REMARK   3      S31:  -0.5149 S32:   0.4918 S33:  -0.0154                       
REMARK   3   TLS GROUP : 22                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 39 THROUGH 45 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -19.8547  55.6292 -18.6959              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7491 T22:   1.5039                                     
REMARK   3      T33:   1.1782 T12:  -0.0450                                     
REMARK   3      T13:  -0.3803 T23:  -0.2237                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.7432 L22:   1.6552                                     
REMARK   3      L33:   7.3120 L12:  -0.4075                                     
REMARK   3      L13:   5.2244 L23:  -0.3135                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3206 S12:   2.2022 S13:  -1.4078                       
REMARK   3      S21:   0.3691 S22:  -0.2244 S23:   1.0142                       
REMARK   3      S31:   0.3760 S32:  -0.6547 S33:   0.0986                       
REMARK   3   TLS GROUP : 23                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 46 THROUGH 51 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -6.4847  60.3471  -9.7029              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3519 T22:   1.0152                                     
REMARK   3      T33:   0.9026 T12:   0.2877                                     
REMARK   3      T13:  -0.3597 T23:   0.0335                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5697 L22:   2.5725                                     
REMARK   3      L33:   2.6580 L12:  -2.4045                                     
REMARK   3      L13:  -0.6757 L23:  -1.5300                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2361 S12:  -0.7395 S13:  -0.3217                       
REMARK   3      S21:  -1.5966 S22:  -0.3127 S23:  -1.1769                       
REMARK   3      S31:   1.4067 S32:   0.5597 S33:   0.7812                       
REMARK   3   TLS GROUP : 24                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 52 THROUGH 66 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.6247  66.7594 -16.2074              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3063 T22:   0.5448                                     
REMARK   3      T33:   1.0282 T12:  -0.0632                                     
REMARK   3      T13:  -0.3584 T23:   0.1226                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.3493 L22:   8.2696                                     
REMARK   3      L33:   4.4095 L12:   1.5659                                     
REMARK   3      L13:  -0.6277 L23:   1.4635                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.9106 S12:   0.8863 S13:  -0.0188                       
REMARK   3      S21:   0.8021 S22:  -0.1445 S23:  -0.4266                       
REMARK   3      S31:  -0.5321 S32:   0.2010 S33:   0.4390                       
REMARK   3   TLS GROUP : 25                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 67 THROUGH 77 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -13.2425  62.1492  -5.8095              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8258 T22:   0.5898                                     
REMARK   3      T33:   0.7157 T12:   0.0738                                     
REMARK   3      T13:  -0.1147 T23:   0.1334                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.7407 L22:   5.2742                                     
REMARK   3      L33:   3.0377 L12:   1.0479                                     
REMARK   3      L13:  -2.0607 L23:   0.2734                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3222 S12:   0.4911 S13:   0.3639                       
REMARK   3      S21:   1.6626 S22:   0.0607 S23:   0.3866                       
REMARK   3      S31:   1.5362 S32:   0.1733 S33:   0.0522                       
REMARK   3   TLS GROUP : 26                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 78 THROUGH 86 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -11.8125  58.4795   5.9786              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.6695 T22:   0.7701                                     
REMARK   3      T33:   0.7351 T12:   0.1846                                     
REMARK   3      T13:  -0.0463 T23:  -0.1447                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.1232 L22:   7.3956                                     
REMARK   3      L33:   3.0547 L12:   2.6736                                     
REMARK   3      L13:  -1.7040 L23:   2.8204                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6969 S12:  -2.4168 S13:   0.2588                       
REMARK   3      S21:   2.4080 S22:  -0.2664 S23:   0.2211                       
REMARK   3      S31:  -0.0518 S32:   0.2171 S33:  -0.0946                       
REMARK   3   TLS GROUP : 27                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 87 THROUGH 93 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -18.8830  69.0336 -16.1950              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5816 T22:   0.2758                                     
REMARK   3      T33:   1.3851 T12:   0.1238                                     
REMARK   3      T13:  -0.2381 T23:   0.5021                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.0041 L22:   7.5313                                     
REMARK   3      L33:   0.5880 L12:  -2.4918                                     
REMARK   3      L13:  -0.2512 L23:   2.0324                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.7417 S12:  -1.2065 S13:  -0.2225                       
REMARK   3      S21:   1.8697 S22:   0.0754 S23:   0.0562                       
REMARK   3      S31:   0.4666 S32:  -0.3781 S33:  -0.2739                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5ECJ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 21-OCT-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000211536.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 13-JUL-14                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : NULL                               
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 19-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97918                            
REMARK 200  MONOCHROMATOR                  : SI (111)                           
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-3000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-3000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 20631                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.050                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 37.764                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 20.00                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 30.0000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.05                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.16                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SAD                          
REMARK 200 SOFTWARE USED: PHENIX 1.9_1692                                       
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 62.09                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.88                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 17% PEG3350, 8% TACSIMATE, PH 8.0,       
REMARK 280  VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+3/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+3/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       90.41500            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       53.40600            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       53.40600            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000      135.62250            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       53.40600            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       53.40600            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       45.20750            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       53.40600            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       53.40600            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000      135.62250            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       53.40600            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       53.40600            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       45.20750            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       90.41500            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, E                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    -1                                                      
REMARK 465     SER A     0                                                      
REMARK 465     GLY A     1                                                      
REMARK 465     LEU A    34                                                      
REMARK 465     VAL A    35                                                      
REMARK 465     PRO A    36                                                      
REMARK 465     THR A    37                                                      
REMARK 465     GLU A    38                                                      
REMARK 465     SER A    39                                                      
REMARK 465     SER A    40                                                      
REMARK 465     GLY A    41                                                      
REMARK 465     SER A    42                                                      
REMARK 465     ASN A    43                                                      
REMARK 465     HIS A    44                                                      
REMARK 465     LEU A    45                                                      
REMARK 465     HIS A    46                                                      
REMARK 465     LYS A    47                                                      
REMARK 465     THR A    48                                                      
REMARK 465     LEU A    49                                                      
REMARK 465     ASP A    50                                                      
REMARK 465     LYS A    51                                                      
REMARK 465     ASP A    52                                                      
REMARK 465     GLU A   190                                                      
REMARK 465     GLY A   191                                                      
REMARK 465     SER A   192                                                      
REMARK 465     SER A   193                                                      
REMARK 465     GLY A   194                                                      
REMARK 465     LYS A   289                                                      
REMARK 465     GLN A   290                                                      
REMARK 465     THR A   291                                                      
REMARK 465     PRO A   292                                                      
REMARK 465     SER A   293                                                      
REMARK 465     GLN A   294                                                      
REMARK 465     TYR A   295                                                      
REMARK 465     LEU A   296                                                      
REMARK 465     ALA A   297                                                      
REMARK 465     GLN A   298                                                      
REMARK 465     HIS A   299                                                      
REMARK 465     GLU A   300                                                      
REMARK 465     LYS A   301                                                      
REMARK 465     LEU A   302                                                      
REMARK 465     LEU A   303                                                      
REMARK 465     LEU A   304                                                      
REMARK 465     ASN A   305                                                      
REMARK 465     THR A   306                                                      
REMARK 465     SER A   307                                                      
REMARK 465     GLY F    -1                                                      
REMARK 465     SER F     0                                                      
REMARK 465     GLY B    -1                                                      
REMARK 465     SER B     0                                                      
REMARK 465     GLY B     1                                                      
REMARK 465     GLY B    32                                                      
REMARK 465     ILE B    33                                                      
REMARK 465     LEU B    34                                                      
REMARK 465     VAL B    35                                                      
REMARK 465     PRO B    36                                                      
REMARK 465     THR B    37                                                      
REMARK 465     GLU B    38                                                      
REMARK 465     SER B    39                                                      
REMARK 465     SER B    40                                                      
REMARK 465     GLY B    41                                                      
REMARK 465     SER B    42                                                      
REMARK 465     ASN B    43                                                      
REMARK 465     HIS B    44                                                      
REMARK 465     LEU B    45                                                      
REMARK 465     HIS B    46                                                      
REMARK 465     LYS B    47                                                      
REMARK 465     THR B    48                                                      
REMARK 465     LEU B    49                                                      
REMARK 465     ASP B    50                                                      
REMARK 465     GLY B   191                                                      
REMARK 465     SER B   192                                                      
REMARK 465     SER B   193                                                      
REMARK 465     ALA B   287                                                      
REMARK 465     ALA B   288                                                      
REMARK 465     LYS B   289                                                      
REMARK 465     GLN B   290                                                      
REMARK 465     THR B   291                                                      
REMARK 465     PRO B   292                                                      
REMARK 465     SER B   293                                                      
REMARK 465     GLN B   294                                                      
REMARK 465     TYR B   295                                                      
REMARK 465     LEU B   296                                                      
REMARK 465     ALA B   297                                                      
REMARK 465     GLN B   298                                                      
REMARK 465     HIS B   299                                                      
REMARK 465     GLU B   300                                                      
REMARK 465     LYS B   301                                                      
REMARK 465     LEU B   302                                                      
REMARK 465     LEU B   303                                                      
REMARK 465     LEU B   304                                                      
REMARK 465     ASN B   305                                                      
REMARK 465     THR B   306                                                      
REMARK 465     SER B   307                                                      
REMARK 465     GLY E    -1                                                      
REMARK 465     SER E     0                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LEU A  26    CG   CD1  CD2                                       
REMARK 470     HIS A  28    CE1  NE2                                            
REMARK 470     ILE A  30    CG1  CG2  CD1                                       
REMARK 470     ILE A  33    CG1  CG2  CD1                                       
REMARK 470     LYS A 101    CG   CD   CE   NZ                                   
REMARK 470     HIS A 103    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ARG A 104    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 114    CG   CD   OE1  OE2                                  
REMARK 470     SER A 195    OG                                                  
REMARK 470     SER A 198    OG                                                  
REMARK 470     LEU A 199    CG   CD1  CD2                                       
REMARK 470     GLU A 228    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 271    CG   CD   CE   NZ                                   
REMARK 470     LYS F   7    CG   CD   CE   NZ                                   
REMARK 470     LEU F   8    CG   CD1  CD2                                       
REMARK 470     GLU F   9    CG   CD   OE1  OE2                                  
REMARK 470     TYR F  78    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ARG F  79    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE B  17    CG1  CG2  CD1                                       
REMARK 470     SER B  19    OG                                                  
REMARK 470     LEU B  26    CG   CD1  CD2                                       
REMARK 470     HIS B  28    CE1  NE2                                            
REMARK 470     SER B  31    OG                                                  
REMARK 470     GLU B  58    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 101    CG   CD   CE   NZ                                   
REMARK 470     LYS B 125    CG   CD   CE   NZ                                   
REMARK 470     HIS B 150    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ARG B 164    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B 253    CG   CD   CE   NZ                                   
REMARK 470     GLU B 256    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 271    CG   CD   CE   NZ                                   
REMARK 470     ARG B 286    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS E   7    CG   CD   CE   NZ                                   
REMARK 470     ARG E  79    CG   CD   NE   CZ   NH1  NH2                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    ALA B   205     NZ   LYS B   210              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU E  18   CA  -  CB  -  CG  ANGL. DEV. =  14.7 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    GLU A  21     -154.97    -82.07                                   
REMARK 500    ALA A  29       79.06    -67.65                                   
REMARK 500    ILE A  30     -169.40    -68.27                                   
REMARK 500    ASP A  79      -95.30   -115.64                                   
REMARK 500    HIS A 103       17.01     58.68                                   
REMARK 500    SER A 119      -69.05    -93.58                                   
REMARK 500    GLN A 151     -122.94     47.26                                   
REMARK 500    ASN A 165       -3.10     74.59                                   
REMARK 500    ARG A 187      132.31    -37.84                                   
REMARK 500    SER A 243       14.58     57.66                                   
REMARK 500    ALA A 287     -105.61     57.26                                   
REMARK 500    GLU B  21     -157.28    -82.40                                   
REMARK 500    ASP B  79      -95.06   -114.44                                   
REMARK 500    SER B 119      -70.56    -87.92                                   
REMARK 500    GLN B 151     -125.19     51.93                                   
REMARK 500    ASN B 165       -1.53     75.28                                   
REMARK 500    THR B 189     -160.74    -72.01                                   
REMARK 500    SER B 243       14.35     58.20                                   
REMARK 500    PRO E  15       -7.36    -52.27                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  5ECJ A    1   190  UNP    E9Q3T6   PRD14_MOUSE    184    373             
DBREF  5ECJ A  199   307  UNP    O70374   MTG8R_MOUSE     98    206             
DBREF  5ECJ F   -1    93  PDB    5ECJ     5ECJ            -1     93             
DBREF  5ECJ B    1   190  UNP    E9Q3T6   PRD14_MOUSE    184    373             
DBREF  5ECJ B  199   307  UNP    O70374   MTG8R_MOUSE     98    206             
DBREF  5ECJ E   -1    93  PDB    5ECJ     5ECJ            -1     93             
SEQADV 5ECJ GLY A   -1  UNP  E9Q3T6              EXPRESSION TAG                 
SEQADV 5ECJ SER A    0  UNP  E9Q3T6              EXPRESSION TAG                 
SEQADV 5ECJ GLY A  191  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER A  192  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER A  193  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ GLY A  194  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER A  195  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER A  196  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ GLY A  197  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER A  198  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ LYS A  301  UNP  O70374    HIS   200 ENGINEERED MUTATION            
SEQADV 5ECJ GLY B   -1  UNP  E9Q3T6              EXPRESSION TAG                 
SEQADV 5ECJ SER B    0  UNP  E9Q3T6              EXPRESSION TAG                 
SEQADV 5ECJ GLY B  191  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER B  192  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER B  193  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ GLY B  194  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER B  195  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER B  196  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ GLY B  197  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ SER B  198  UNP  E9Q3T6              LINKER                         
SEQADV 5ECJ LYS B  301  UNP  O70374    HIS   200 ENGINEERED MUTATION            
SEQRES   1 A  309  GLY SER GLY PHE ASN PHE THR GLU GLU GLU LEU SER PHE          
SEQRES   2 A  309  VAL LEU TYR GLY ALA ILE ALA SER PRO GLU HIS PRO THR          
SEQRES   3 A  309  ASP LEU GLN HIS ALA ILE SER GLY ILE LEU VAL PRO THR          
SEQRES   4 A  309  GLU SER SER GLY SER ASN HIS LEU HIS LYS THR LEU ASP          
SEQRES   5 A  309  LYS ASP SER LEU GLN LEU PRO GLU GLY LEU CYS LEU MET          
SEQRES   6 A  309  GLN THR SER PHE GLY ASP VAL PRO HIS PHE GLY VAL PHE          
SEQRES   7 A  309  CYS SER ASP PHE ILE ALA LYS GLY VAL ARG PHE GLY PRO          
SEQRES   8 A  309  PHE ARG GLY ARG VAL VAL ASN ALA SER GLU VAL LYS ALA          
SEQRES   9 A  309  HIS ARG ASP ASN SER ARG MET TRP GLU ILE PHE GLU ASP          
SEQRES  10 A  309  GLY HIS LEU SER HIS PHE ILE ASP GLY LYS GLY SER GLY          
SEQRES  11 A  309  ASN TRP MET SER TYR VAL ASN CYS ALA ARG PHE PRO LYS          
SEQRES  12 A  309  GLU GLN ASN LEU LEU ALA VAL GLN HIS GLN GLY GLN ILE          
SEQRES  13 A  309  PHE TYR GLU SER CYS ARG ASP ILE GLN ARG ASN GLN GLU          
SEQRES  14 A  309  LEU LEU VAL TRP TYR GLY ASN GLY TYR GLU LYS PHE LEU          
SEQRES  15 A  309  GLY VAL PRO MET ASN LEU ARG VAL THR GLU GLY SER SER          
SEQRES  16 A  309  GLY SER SER GLY SER LEU PRO ALA THR CYS GLY ALA ARG          
SEQRES  17 A  309  GLN LEU SER LYS LEU LYS ARG PHE LEU THR THR LEU GLN          
SEQRES  18 A  309  GLN PHE GLY ASN ASP ILE SER PRO GLU ILE GLY GLU LYS          
SEQRES  19 A  309  VAL ARG THR LEU VAL LEU ALA LEU VAL ASN SER THR VAL          
SEQRES  20 A  309  THR ILE GLU GLU PHE HIS CYS LYS LEU GLN GLU ALA THR          
SEQRES  21 A  309  ASN PHE PRO LEU ARG PRO PHE VAL ILE PRO PHE LEU LYS          
SEQRES  22 A  309  ALA ASN LEU PRO LEU LEU GLN ARG GLU LEU LEU HIS CYS          
SEQRES  23 A  309  ALA ARG ALA ALA LYS GLN THR PRO SER GLN TYR LEU ALA          
SEQRES  24 A  309  GLN HIS GLU LYS LEU LEU LEU ASN THR SER                      
SEQRES   1 F   95  GLY SER VAL SER SER VAL PRO THR LYS LEU GLU VAL VAL          
SEQRES   2 F   95  ALA ALA THR PRO THR SER LEU LEU ILE SER TRP ASP ALA          
SEQRES   3 F   95  PRO ALA VAL THR VAL ASP LEU TYR PHE ILE THR TYR GLY          
SEQRES   4 F   95  GLU THR GLY GLY ASN SER PRO VAL GLN LYS PHE THR VAL          
SEQRES   5 F   95  PRO GLY SER LYS SER THR ALA THR ILE SER GLY LEU LYS          
SEQRES   6 F   95  PRO GLY VAL ASP TYR THR ILE THR VAL TYR ALA GLN TYR          
SEQRES   7 F   95  TYR TYR ARG GLY TRP TYR VAL GLY SER PRO ILE SER ILE          
SEQRES   8 F   95  ASN TYR ARG THR                                              
SEQRES   1 B  309  GLY SER GLY PHE ASN PHE THR GLU GLU GLU LEU SER PHE          
SEQRES   2 B  309  VAL LEU TYR GLY ALA ILE ALA SER PRO GLU HIS PRO THR          
SEQRES   3 B  309  ASP LEU GLN HIS ALA ILE SER GLY ILE LEU VAL PRO THR          
SEQRES   4 B  309  GLU SER SER GLY SER ASN HIS LEU HIS LYS THR LEU ASP          
SEQRES   5 B  309  LYS ASP SER LEU GLN LEU PRO GLU GLY LEU CYS LEU MET          
SEQRES   6 B  309  GLN THR SER PHE GLY ASP VAL PRO HIS PHE GLY VAL PHE          
SEQRES   7 B  309  CYS SER ASP PHE ILE ALA LYS GLY VAL ARG PHE GLY PRO          
SEQRES   8 B  309  PHE ARG GLY ARG VAL VAL ASN ALA SER GLU VAL LYS ALA          
SEQRES   9 B  309  HIS ARG ASP ASN SER ARG MET TRP GLU ILE PHE GLU ASP          
SEQRES  10 B  309  GLY HIS LEU SER HIS PHE ILE ASP GLY LYS GLY SER GLY          
SEQRES  11 B  309  ASN TRP MET SER TYR VAL ASN CYS ALA ARG PHE PRO LYS          
SEQRES  12 B  309  GLU GLN ASN LEU LEU ALA VAL GLN HIS GLN GLY GLN ILE          
SEQRES  13 B  309  PHE TYR GLU SER CYS ARG ASP ILE GLN ARG ASN GLN GLU          
SEQRES  14 B  309  LEU LEU VAL TRP TYR GLY ASN GLY TYR GLU LYS PHE LEU          
SEQRES  15 B  309  GLY VAL PRO MET ASN LEU ARG VAL THR GLU GLY SER SER          
SEQRES  16 B  309  GLY SER SER GLY SER LEU PRO ALA THR CYS GLY ALA ARG          
SEQRES  17 B  309  GLN LEU SER LYS LEU LYS ARG PHE LEU THR THR LEU GLN          
SEQRES  18 B  309  GLN PHE GLY ASN ASP ILE SER PRO GLU ILE GLY GLU LYS          
SEQRES  19 B  309  VAL ARG THR LEU VAL LEU ALA LEU VAL ASN SER THR VAL          
SEQRES  20 B  309  THR ILE GLU GLU PHE HIS CYS LYS LEU GLN GLU ALA THR          
SEQRES  21 B  309  ASN PHE PRO LEU ARG PRO PHE VAL ILE PRO PHE LEU LYS          
SEQRES  22 B  309  ALA ASN LEU PRO LEU LEU GLN ARG GLU LEU LEU HIS CYS          
SEQRES  23 B  309  ALA ARG ALA ALA LYS GLN THR PRO SER GLN TYR LEU ALA          
SEQRES  24 B  309  GLN HIS GLU LYS LEU LEU LEU ASN THR SER                      
SEQRES   1 E   95  GLY SER VAL SER SER VAL PRO THR LYS LEU GLU VAL VAL          
SEQRES   2 E   95  ALA ALA THR PRO THR SER LEU LEU ILE SER TRP ASP ALA          
SEQRES   3 E   95  PRO ALA VAL THR VAL ASP LEU TYR PHE ILE THR TYR GLY          
SEQRES   4 E   95  GLU THR GLY GLY ASN SER PRO VAL GLN LYS PHE THR VAL          
SEQRES   5 E   95  PRO GLY SER LYS SER THR ALA THR ILE SER GLY LEU LYS          
SEQRES   6 E   95  PRO GLY VAL ASP TYR THR ILE THR VAL TYR ALA GLN TYR          
SEQRES   7 E   95  TYR TYR ARG GLY TRP TYR VAL GLY SER PRO ILE SER ILE          
SEQRES   8 E   95  ASN TYR ARG THR                                              
HELIX    1 AA1 THR A    5  GLY A   15  1                                  11    
HELIX    2 AA2 ASN A  129  VAL A  134  5                                   6    
HELIX    3 AA3 PHE A  139  GLN A  143  5                                   5    
HELIX    4 AA4 ARG A  206  SER A  226  1                                  21    
HELIX    5 AA5 SER A  226  ASN A  242  1                                  17    
HELIX    6 AA6 THR A  246  ASN A  259  1                                  14    
HELIX    7 AA7 PHE A  265  HIS A  283  1                                  19    
HELIX    8 AA8 THR B    5  GLY B   15  1                                  11    
HELIX    9 AA9 LYS B   51  GLN B   55  5                                   5    
HELIX   10 AB1 ASN B  129  VAL B  134  5                                   6    
HELIX   11 AB2 PHE B  139  GLN B  143  5                                   5    
HELIX   12 AB3 ARG B  206  SER B  226  1                                  21    
HELIX   13 AB4 SER B  226  ASN B  242  1                                  17    
HELIX   14 AB5 THR B  246  ASN B  259  1                                  14    
HELIX   15 AB6 PHE B  265  HIS B  283  1                                  19    
SHEET    1 AA1 2 ALA A  16  ALA A  18  0                                        
SHEET    2 AA1 2 THR A  24  LEU A  26 -1  O  ASP A  25   N  ILE A  17           
SHEET    1 AA2 2 LEU A  60  PHE A  67  0                                        
SHEET    2 AA2 2 VAL A  70  CYS A  77 -1  O  GLY A  74   N  MET A  63           
SHEET    1 AA3 5 ARG A  86  PHE A  87  0                                        
SHEET    2 AA3 5 GLN A 153  SER A 158 -1  O  TYR A 156   N  PHE A  87           
SHEET    3 AA3 5 LEU A 145  HIS A 150 -1  N  VAL A 148   O  PHE A 155           
SHEET    4 AA3 5 LEU A 169  TYR A 172  1  O  TRP A 171   N  ALA A 147           
SHEET    5 AA3 5 ASN A 135  CYS A 136  1  N  ASN A 135   O  VAL A 170           
SHEET    1 AA4 3 ARG A  93  ASN A  96  0                                        
SHEET    2 AA4 3 HIS A 117  ASP A 123 -1  O  PHE A 121   N  VAL A  95           
SHEET    3 AA4 3 MET A 109  GLU A 114 -1  N  ILE A 112   O  SER A 119           
SHEET    1 AA5 2 GLU A 177  PHE A 179  0                                        
SHEET    2 AA5 2 VAL A 182  ASN A 185 -1  O  MET A 184   N  GLU A 177           
SHEET    1 AA6 3 THR F   6  THR F  14  0                                        
SHEET    2 AA6 3 SER F  17  ASP F  23 -1  O  SER F  17   N  THR F  14           
SHEET    3 AA6 3 THR F  56  SER F  60 -1  O  ILE F  59   N  LEU F  18           
SHEET    1 AA7 4 GLN F  46  PRO F  51  0                                        
SHEET    2 AA7 4 VAL F  29  GLU F  38 -1  N  TYR F  32   O  VAL F  50           
SHEET    3 AA7 4 ASP F  67  TYR F  77 -1  O  TYR F  73   N  PHE F  33           
SHEET    4 AA7 4 TRP F  81  VAL F  83 -1  O  TYR F  82   N  TYR F  76           
SHEET    1 AA8 4 GLN F  46  PRO F  51  0                                        
SHEET    2 AA8 4 VAL F  29  GLU F  38 -1  N  TYR F  32   O  VAL F  50           
SHEET    3 AA8 4 ASP F  67  TYR F  77 -1  O  TYR F  73   N  PHE F  33           
SHEET    4 AA8 4 ILE F  87  ARG F  92 -1  O  TYR F  91   N  TYR F  68           
SHEET    1 AA9 2 ILE B  17  ALA B  18  0                                        
SHEET    2 AA9 2 THR B  24  ASP B  25 -1  O  ASP B  25   N  ILE B  17           
SHEET    1 AB1 2 LEU B  60  PHE B  67  0                                        
SHEET    2 AB1 2 VAL B  70  CYS B  77 -1  O  GLY B  74   N  MET B  63           
SHEET    1 AB2 5 ARG B  86  PHE B  87  0                                        
SHEET    2 AB2 5 GLN B 153  SER B 158 -1  O  TYR B 156   N  PHE B  87           
SHEET    3 AB2 5 LEU B 145  HIS B 150 -1  N  VAL B 148   O  PHE B 155           
SHEET    4 AB2 5 LEU B 169  TYR B 172  1  O  TRP B 171   N  ALA B 147           
SHEET    5 AB2 5 ASN B 135  CYS B 136  1  N  ASN B 135   O  VAL B 170           
SHEET    1 AB3 3 ARG B  93  ASN B  96  0                                        
SHEET    2 AB3 3 HIS B 117  ASP B 123 -1  O  PHE B 121   N  VAL B  95           
SHEET    3 AB3 3 MET B 109  GLU B 114 -1  N  GLU B 114   O  HIS B 117           
SHEET    1 AB4 3 TYR B 176  PHE B 179  0                                        
SHEET    2 AB4 3 VAL B 182  LEU B 186 -1  O  MET B 184   N  GLU B 177           
SHEET    3 AB4 3 GLY B 197  SER B 198 -1  O  GLY B 197   N  LEU B 186           
SHEET    1 AB5 3 THR E   6  THR E  14  0                                        
SHEET    2 AB5 3 SER E  17  ASP E  23 -1  O  SER E  21   N  GLU E   9           
SHEET    3 AB5 3 THR E  56  ILE E  59 -1  O  ALA E  57   N  ILE E  20           
SHEET    1 AB6 4 GLN E  46  PRO E  51  0                                        
SHEET    2 AB6 4 VAL E  29  GLU E  38 -1  N  ILE E  34   O  PHE E  48           
SHEET    3 AB6 4 ASP E  67  TYR E  77 -1  O  GLN E  75   N  LEU E  31           
SHEET    4 AB6 4 TRP E  81  VAL E  83 -1  O  TYR E  82   N  TYR E  76           
SHEET    1 AB7 4 GLN E  46  PRO E  51  0                                        
SHEET    2 AB7 4 VAL E  29  GLU E  38 -1  N  ILE E  34   O  PHE E  48           
SHEET    3 AB7 4 ASP E  67  TYR E  77 -1  O  GLN E  75   N  LEU E  31           
SHEET    4 AB7 4 ILE E  87  ARG E  92 -1  O  ILE E  87   N  VAL E  72           
CISPEP   1 GLY A   88    PRO A   89          0         6.49                     
CISPEP   2 GLY B   88    PRO B   89          0         4.62                     
CRYST1  106.812  106.812  180.830  90.00  90.00  90.00 P 43 21 2    16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009362  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.009362  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.005530        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system